Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*11,073,7610CA36.7% 94.2 / 12.5 49L191I (CTT→ATT) unknown4‑hydroxy‑2‑oxoglutarate aldolase (EC 4.1.3.16) @ 2‑dehydro‑3‑deoxyphosphogluconate aldolase (EC 4.1.2.14)
Reads supporting (aligned to +/- strand):  ref base C (10/21);  new base A (18/0);  total (28/21)
Fisher's exact test for biased strand distribution p-value = 1.82e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

TGCCCATCACACCGGGGGTAAACAATCCGATGGCAATTGAAGCCGCGTTGGACGTCGGTATTGAAACCGTGAAATTCTTCCCGGCGGAAGCCTCTGGTGGCGTGAAAATGATTAAAGCCTTGTTAGGCCCTTACGCACAGTTGCAAATTATGCCGACCGGCGGTATCGGTATGCAGAATATTAAAGACTATCTGGCAATTCCCAATGTGGTGGCATGTGGC‑GGTTC‑TTGGTTTGT‑AGAAAAGAAAC‑TTATCAATG‑AAAAAAGCTGGCA‑GGAAATCGGT‑C‑GGTTAA‑CCAAAGAGGTGGC‑GGAATGGATCAACACTTAATCATGAATTAATCAATAAACTCAACCGCACTTCAACATCAATGTTAAAGTGCGGTGGTTTTTCGTTGCGTTTTTATAACGCGCTATTATTTTGCTGCTTTATAAGGCTGGCGATAGAATTTCAAATAATAGTCGTTAACCATTTTATCTAAATCCACATCGCGAAAAAGC  >  1/1073516‑1074002
                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                        
tGCCCATCACACCGGGGGTAAACAATCCGATGGCAATTGAAGCCGCGTTGGACGTCGGTATTGAAACCGTGAAATTCTTCCCGGCGGAAGCCTCTGGTGGCGTGAAAATGATTAAAGCCTTGTTAGGCCCTTACGCACAGTTGCAAATTATGCCGACCGGCGGTATCGGTATGCAGAATATTAAAGACTATCTGGCAATTCCCAATGTGGTGGCATGTGGC‑GGTTC‑TTGGTTTGT‑AGAAAAGAAAC‑TTATc                                                                                                                                                                                                                                                    <  1:203508/251‑1 (MQ=255)
   ccATCACACCGGGGGTAAACAATCCGATGGCAATTGAAGCCGCGTTGGACGTCGGTATTGAAACCGTGAAATTCTTCCCGGCGGAAGCCTCTGGTGGCGTGAAAATGATTAAAGCCTTGTTAGGCCCTTACGCACAGTTGCAAATTATGCCGACCGGCGGTATCGGTATGCAGAATATTAAAGACTATCTGGCAATTCCCAATGTGGTGGCATGTGGC‑GGGTT‑TTTGTTTGT‑TGAAAAGAAAA‑TTAttaaa                                                                                                                                                                                                                                                 >  1:510582/1‑247 (MQ=255)
              ggggTAAACAATCCGATGGCAATTGAAGCCGCGTTGGACGTCGGTATTGAAACCGTGAAATTCTTCCCGGCGGAAGCCTCTGGTGGCGTGAAAATGATTAAAGCCTTGTTAGGCCCTTACGCACAGTTGCAAATTATGCCGACCGGCGGTATCGGTATGCAGAATATTAAAGACTATCTGGCAATTCCCAATGTGGTGGCATGTGGCGGGTTT‑TTGGTTTTT‑GGAAAA‑AAAA‑TTATCAATG‑aaaaaaaatg                                                                                                                                                                                                                                     >  1:279943/1‑247 (MQ=255)
              ggggTAAACAATCCGATGGCAATTGAAGCCGCGTTGGACGTCGGTATTGAAACCGTGAAATTCTTCCCGGCGGAAGCCTCTGGTGGCGTGAAAATGATTAAAGCCTTGTTAGGCCCTTACGCACAGTTGCAAATTATGCCGACCGGCGGTATCGGTATGCAGAATATTAAAGACTATCTGGCAATTCCCAATGTGGTGGCATGTGGCGGGTTT‑TTGG‑TTGT‑AGAAAAAAAAA‑TTATTAATG‑aaaaaaaatg                                                                                                                                                                                                                                     >  1:646735/1‑247 (MQ=255)
                             atgcctgaagcgattgcgTTGGACGTCGGTATTGAAACCGTGAAATTCTTCCCGGCGGAAGCCTCTGGTGGCGTGAAAATGATTAAAGCCTTGTTAGGCCCTTACGCACAGTTGCAAATTATGCCGACCGGCGGTATCGGTATGCAGAATATTAAAGACTATCTGGCAATTCCCAATGTGGTGGCATGTGGC‑GGTCC‑TTTGTTTTT‑AGAAAAAAAAA‑TTTTTAAAG‑AAAAAAACTGGCA‑gggaaacgggcg                                                                                                                                                                                                                     >  1:407863/16‑241 (MQ=255)
                                 cAATTGAAGCCGCGTTGGACGTCGGTATTGAAACCGTGAAATTCTTCCCGGCGGAAGCCTCTGGTGGCGTGAAAATGATTAAAGCCTTGTTAGGCCCTTACGCACAGTTGCAAATTATGCCGACCGGCGGTATCGGTATGCAGAATATTAAAGACTATCTGGCAATTCCCAATGTGGTGGCATGTGGC‑GGTTC‑TTTGTTTTT‑TGAAAAGAAAA‑TTATTAATG‑AAAAAAGCTGGCA‑GGGAAtcggg‑c‑gggta                                                                                                                                                                                                               >  1:195565/1‑248 (MQ=255)
                                                         gTATTGAAACCGTGAAATTCTTCCCGGCGGAAGCCTCTGGTGGCGTGAAAATGATTAAAGCCTTGTTAGGCCCTTACGCACAGTTGCAAATTATGCCGACCGGCGGTATCGGTATGCAGAATATTAAAGACTATCTGGCAATTCCCAATGTGGTGGCATGTGGC‑GGTTC‑TTGGTTTGT‑AGAAAAGAAAC‑TTATCAATG‑AAAAAAGCTGGCA‑GGAAATCGGT‑C‑GGTTAA‑CCAAAGAGGTGGC‑GGAATGGAt                                                                                                                                                                                      <  1:20132/250‑1 (MQ=255)
                                                         gTATTGAAACCGTGAAATTCTTCCCGGCGGAAGCCTCTGGTGGCGTGAAAATGATTAAAGCCTTGTTAGGCCCTTACGCACAGTTGCAAATTATGCCGACCGGCGGTATCGGTATGCAGAATATTAAAGACTATCTGGCAATTCCCAATGTGGTGGCATGTGGC‑GGGTT‑TTTGTTTGT‑TGGAAAGAAAA‑TTATTAATG‑AAAAAAAATGGCA‑GGGAAACGGG‑CGGGTTAA‑‑CAAAAAGGGGGG‑GGAATGGAtt                                                                                                                                                                                     >  1:164993/1‑250 (MQ=255)
                                                         gTATTGAAACCGTGAAATTCTTCCCGGCGGAAGCCTCTGGTGGCGTGAAAATGATTAAAGCCTTGTTAGGCCCTTACGCACAGTTGCAAATTATGCCGACCGGCGGTATCGGTATGCAGAATATTAAAGACTATCTGGCAATTCCCAATGTGGTGGCATGTGGC‑GGGTCTTTGGTTT‑T‑TGAAAAAAAAC‑ATTTTAATG‑AAAAAAAATGGGA‑GGAAAACGGG‑C‑GGTTAA‑CCAAAAAGGGGGC‑gggaaggata                                                                                                                                                                                     >  1:598936/1‑243 (MQ=255)
                                                         gTATTGAAACCGTGAAATTCTTCCCGGCGGAAGCCTCTGGTGGCGTGAAAATGATTAAAGCCTTGTTAGGCCCTTACGCACAGTTGCAAATTATGCCGACCGGCGGTATCGGTATGCAGAATATTAAAGACTATCTGGCAATTCCCAATGTGGTGGCATGTGGC‑GGGTC‑TTTGTTTGT‑TGAAAAAAAAA‑CTATTAATG‑AAAAAAAATGGGA‑GGGAAAAGGT‑C‑GGGTAA‑CCAAAAAAGGGGG‑GGAATGGAtt                                                                                                                                                                                     >  1:368602/1‑250 (MQ=255)
                                                            ttGAAACCGTGAAATTCTTCCCGGCGGAAGCCTCTGGTGGCGTGAAAATGATTAAAGCCTTGTTAGGCCCTTACGCACAGTTGCAAATTATGCCGACCGGCGGTATCGGTATGCAGAATATTAAAGACTATCTGGCAATTCCCAATGTGGTGGCATGTGGC‑GGTTC‑TTGGTTTGT‑AGAAAAGAAAC‑TTATCAATG‑AAAAAAGCTGGCA‑GGAAATCGGT‑C‑GGTTAA‑CCAAAGAGGTGGC‑GGAATGGATCAac                                                                                                                                                                                  <  1:114377/251‑1 (MQ=255)
                                                                   cGTGAAATTCTTCCCGGCGGAAGCCTCTGGTTGCGTGAAAATGATTAAAGCCTTGTTAGGCCCTTACGCACAGTTGCAAATTATGCCGACCGGCGGTATCGGTATGCAGAATATTAAAGACTATCTGGCAATTCCCAATGTGGTGGCATGTGGC‑GGTTC‑TTGGTTTGT‑ATAAAAGAAAC‑TTATCAATG‑AAAATAGCTGGCA‑GGAAATCGGT‑C‑GGTGAA‑CCAAAGATGTGGC‑GGAATGGATCAACACTTAAt                                                                                                                                                                           <  1:591004/251‑1 (MQ=255)
                                                                          ttcttcCCGGCGGAAGCCTCTGGTGGCGTGAAAATGATTAAAGCCTTGTTAGGCCCTTACGCACAGTTGCAAATTATGCCGACCGGCGGTATCGGTATGCAGAATATTAAAGACTATCTGGCAATTCCCAATGTGGTGGCATGTGGC‑GGTTC‑TTGGTTTGT‑AGAAAAGAAAC‑TTATCAATG‑AAAAAAGCTGGCA‑GGAAATCGGT‑C‑GGTTAA‑CCAAAGAGGTGGC‑GGAATGGATCAACACTTAATCATGAAt                                                                                                                                                                    <  1:108412/251‑1 (MQ=255)
                                                                                                                    gCCTTGTTAGGCCCTTACGCACAGTTGCAAATTATGCCGACCGGCGGTATCGGTATGCAGAATATTAAAGACTATCTGGCAATTCCCAATGTGGTGGCATGTGGC‑GGGTCTTTGGTTT‑T‑AGAAAAAAAAA‑TTATCAATG‑AAAAAAACTGGCA‑GGGAAACGGT‑CGGGTAAA‑CCAAA‑AGGGGGG‑GGAAAGGATCAACACTTAAACATGAATTAATc                                                                                                                                                               >  1:65934/1‑214 (MQ=255)
                                                                                                                        tGTTAGGCCCTTACGCACAGTTGCAAATTATGCCGACCGGCGGTATCGGTATGCAGAATATTAAAGACTATCTGGCAATTCCCAATGTGGTGGCATGTGGCGGGTTT‑TTGGTTT‑T‑TGAAAAGAAACTTTTTCAA‑G‑AAAAAAAGTGGGA‑GGGAAACGGG‑C‑GGGTAACCCAAAAAGGGGGG‑GGAA‑GGATCAACACATAAACATGAATTTATTAATAAAAACAACCGCACCTCaaacacaaagtttaaatgcgg                                                                                                                      >  1:169712/1‑232 (MQ=255)
                                                                                                                        tGTTAGGCCCTTACGCACAGTTGCAAATTATGCCGACCGGCGGTATCGGTATGCAGAATATTAAAGACTATCTGGCAATTCCCAATGTGGTGGCATGTGGC‑GGTTC‑TTGGTTTGT‑AGAAAAGAAAC‑TTATCAATG‑AAAAAAGCTGGCA‑GGAAATCGGT‑C‑GGTTAA‑CCAAAGAGGTGGC‑GGAATGGATCAACACTTAATCATGAATTAATCAATAAACTCAACCGCACTTc                                                                                                                                           <  1:108005/230‑1 (MQ=255)
                                                                                                                                    aCGCACAGTTGCAAATTATGCCGACCGGCGGTATCGGTATGCAGAATATTAAAGACTATCTGGCAATTCCCCATGTGGTGGCATGTGGC‑GGTTC‑TTTGTTTTT‑AGAAAAGAAAA‑TTTTTAATGAAAAAAAGC‑GGCAGGGAAATGGGC‑C‑GTTAAA‑CCAAAAAGG‑GGCGGGAAT‑GATCAACCCATAATACTGAAGTTATTACAAAAATCAACCTCCCTTCTACATCAATGTTAAAATGCGGGGGgttttttgt                                                                                                          >  1:47886/1‑247 (MQ=255)
                                                                                                                                    aCGCACAGTTGCAAATTATGCCGACCGGCGGTATCGGTATGCAGAATATTAAAGACTATCTGGCAATTCCCAATGTGGTGGCATGTGGC‑GGTTC‑TTTGTTTTT‑TGAAAAAAAAA‑ATTTTAATG‑AAAAAAAAAGGGA‑GGGAAACGGG‑C‑GGGTAA‑ACAAAAAGGGGGG‑GGAAAGGATCAACACCTAAATATTAAATAATCAAAAAAATCAACCGCACCTCAAAAACAAAGTTAAAATGCGGGGGgtttttg                                                                                                            >  1:602693/1‑247 (MQ=255)
                                                                                                                                    aCGCACAGTTGCAAATTATGCCGACCGGCGGTATCGGTATGCAGAATATTAAAGACTATCTGGCAATTCCCAATGTGGTGGCATGTGGC‑GGGTC‑TTGGGTTTT‑GGAAAAAAAAA‑TTTTTAATT‑AAAAAAGCCGGCG‑GGAAAAAGGG‑C‑GGGTAA‑ACAAAAAGGGGGC‑GGGATGGAACAAAACATAATCATGAaataata                                                                                                                                                               >  1:44606/1‑197 (MQ=255)
                                                                                                                                         caGTTGCAAATTATGCCGACCGGCGGTATCGGTATGCAGAATATTAAAGACTATCTGGCAATTCCCAATGTGGTGGCATGTGGC‑GGTTA‑TTGGTTTGT‑AGAAAAGAAAC‑TTATCAATG‑AAAAAAGCTGGCA‑GGAAAtcggt                                                                                                                                                                                                                       <  1:120827/141‑1 (MQ=255)
                                                                                                                                         caGTTGCAAATTATGCCGACCGGCGGTATCGGTATGCAGAATATTAAAGACTATCTGGCAATTCCCAATGTGGTGGCATGTGGC‑GGGTC‑TTTGTTTTT‑AGAAAAAAAAA‑TTTTTAATG‑AAAAAAAATGGGAGGGAAAT‑GGG‑C‑GGGTAA‑CCAAAAAGGGGGG‑GGGAAGGGTCAAAAACTAATTATGAAATAAACAAAAAAATCAACCGCACTTCAACATCAATGTTAAAATGCGGGGGGTTTTTTTTGCGtt                                                                                                     >  1:302682/1‑251 (MQ=255)
                                                                                                                                           gTTGCAAATTATGCCGACCGGCGGTATCGGTATGCAGAATATTAAAGACTATCTGGCAATTCCCAATGTGGTGGCATGTGGC‑GGTTC‑TTGGTTTGT‑AGAAAAGAAAC‑TTATCAATG‑AAAAAAGCTGGCA‑GGAAATCGGT‑C‑GGTTAA‑CCAAAGAGGTGGC‑GGAAt                                                                                                                                                                                          <  1:486303/164‑1 (MQ=255)
                                                                                                                                           gTTGCAAATTATGCCGACCGGCGGTATCGGTATGCAGAATATTAAAGACTATCTGGCAATTCCCAATGTGGTGGCATGTGGC‑GGCTCTTTGGTTT‑T‑AGAAAAGAAAC‑TTTTCAAAG‑AAAAAAGCTGGCA‑GGAAAACGGG‑CGGGTAAA‑CCAAAG‑GGGGGC‑GGAATGGGTCAACAACTAATCATGAAATAATCAAAAAAATCAACCCCACTTCAACATCAATGTTAAAATGCGGGGGgtttttttttcgtttt                                                                                                   >  1:79593/1‑240 (MQ=255)
                                                                                                                                                aaaTTATGCCGACCGGCGGTATCGGTATGCAGAATATTAAAGACTATCTGGCAATTCCCAATGTGGTGGCATGTGGC‑GGGTC‑TTTGTTTGT‑AGAAAAAAAAA‑TTATCAATG‑AAAAAAACCGGCC‑GGAAAACGGT‑C‑GGGTAA‑ACAAAGAGGGGGC‑GGAAAAGATCAAAACCTAATCATTAATTTAACAATAAAATCAACCGCACTTCAACATCAAAGTTAAAATGCGGGGGTTTTTTGTTTGGTTTTtatta                                                                                              >  1:363886/1‑249 (MQ=255)
                                                                                                                                                          gACCGGCGGTATCGGTATGCAGAATATTAAAGACTATCTGGCAATTCCCAATGTGGTGGCATGTGGC‑GGTTT‑TTTGTTTGT‑AGAAAAAAAAA‑TTTTCAATG‑AAAAAAACTGGGA‑GGGAAACGGT‑CGGGTTAA‑‑CAAAAAGGGGGG‑GGAAAGGGTCAAAAATTTAACATGAAATAAATAATAAAAACAAACGCACCTCAACAACAATGTTTAAGTGCGGGGGGTTTTTTTTTCGGTTTTTTAAAGCGCtattt                                                                                    >  1:354390/1‑250 (MQ=255)
                                                                                                                                                                    atcggtatGCAGAATATTAAAGACTATCTGGCAATTCCCAATGTGGTGGCATGTGGC‑GGTTC‑TTGGTTTGT‑AGAAAAGAAAA‑TTATCAATG‑AAAAAAGCTGGCA‑GGAAATCGGT‑C‑GGTTAA‑ACAAAGAGGTGGC‑GGAATGGATCAACACTTAATCATGAATTAATCAATAAACTCAACCGCACTTCAACATCAATGTTAAAGTGCGGGGGGTTTTCGTTGCGTTTTTATAACGCGCTATTATTTttctg                                                                            >  1:12482/1‑249 (MQ=255)
                                                                                                                                                                       ggtatGCAGAATATTAAAGACTATCTGGCAATTCCCAATGTGGTGGCATGTGGC‑GGTTC‑TTGGTTTGT‑AGAAAAGAAAC‑TTATCAATG‑AAAAAAGCTGGCA‑GGAAATCGGG‑C‑GGTTAA‑CCAAAGAGGGGGC‑GGAATGGATCAACACTTAATCATGAATTAATCAATAAACTCAACCGCACTTCAACATCAATGTTAAAGTGCGGGGGTTTTTCGTTGCGTTTTTATAACGCGCTATTATTTTTCTGCtttt                                                                       >  1:245742/1‑250 (MQ=255)
                                                                                                                                                                        gtatGCAGAATATTAAAGACTATCTGGCAATTCCCAATGTGGTGGCATGTGGC‑GGTTC‑TTGGTTTGT‑AGAAAAGAAAC‑TTATCAATG‑AAAAAAGCTGGCA‑GGAAATCGGT‑C‑GGTTAA‑CCAAAGAGGTGGC‑GGa                                                                                                                                                                                            <  1:472132/133‑1 (MQ=255)
                                                                                                                                                                            gCAGAATATTAAAGACTATCTGGCAATTCCCAATGTGGTGGCATGTGGC‑GGTTT‑TTGGTTTGT‑AGAAAAGAAAC‑TTATCAATG‑AAAAAAGCTGGCA‑GGAAATCGGT‑C‑GGTTAA‑ACAAAAAGGGGGG‑GGAATGGAACAAAACTTAATCATGAATTAATCAATAAACTCAACCGCACTTCAAAATCAATGTTAAAGAGCGGGGGGTTTTTGTTGCGTTTTTTTAAAGCGCTATTATTTTTCTGCTTTTTAAgg                                                                  >  1:506825/1‑251 (MQ=255)
                                                                                                                                                                                aaTATTAAAGACTATCTGGCAATTCCCAATGTGGTGGCATGTGGC‑GGTTC‑TTGGTTTGT‑AGAAAAGAAAC‑TTATCAATG‑AAAAAAGCTGGCA‑GGAAATCGGT‑C‑GGTTAA‑CCAAAGAGGTGGC‑GGAATGGATCAACACTTAATCATGAATTAATCAATAAACTCAACCGCACTTCAACATCAATGTTAAAGTGCGGTGGTTTTTCGTTGCGTTTTTATAACGCGCTATTATTTTGCTGCTTTATAAGGc                                                                 <  1:301002/248‑1 (MQ=255)
                                                                                                                                                                                aaTATTAAAGACTATCTGGCAATTCCCAATGTGGTGGCATGTGGC‑GGTTC‑TTGGTTTGT‑AGAAAAGAAAC‑TTATCAATG‑AAAAAAGCTGGCA‑GGAAATCGGT‑C‑GGTTAA‑CCAAAGAGGTGGC‑GGAATGGATCAACACTTAATCATGAATTAATCAATAAACTCAACCGCACTTCAACATCAATGTTAAAGTGCGGTGGTTTTTCGTTGCGTTTTTATAACGCGCTATTATTTTGCTGCTTTATAAGGc                                                                 <  1:582456/248‑1 (MQ=255)
                                                                                                                                                                                 atatTAAAGACTATCTGGCAATTCCCAATGTGGTGGCATGTGGC‑GGTTC‑TTGGTTTGT‑AGAAAAGAAAC‑TTATCAATG‑AAAAAAGCTGGCA‑GGAAATCGGT‑C‑GGTTAA‑CCAAAGAGGTGGC‑GGAATGGATCAACACTTAATCATGAATTAATCAATAAACTCAACCGCACTTCAACATCAATGTTAAAGTGCGGTGGTTTTTCGTTGCGTTTTTATAACGCGCTATTATTTTGCTGCTTTATaa                                                                    <  1:397472/244‑1 (MQ=255)
                                                                                                                                                                                 atatTAAAGACTATCTGGCAATTCCCAATGTGGTGGCATGTGGC‑GGTTA‑TTTGTTTGTAAGAAAA‑AAAC‑TTATCAATG‑AAAAAAGCTGGCC‑GGAAAACCGT‑G‑GGTTAA‑ACAAAGAGGGGGC‑GGAAGGGGTAAAAACTTAATCATGAATTAATCAATAAACTCAACCGCACTTCAAAATCAAAGTTAAAAGGCGGTGGTTTTTCCTTTCGTTTTTATAACGCGCTATTATTTTGCTACTTTATaaaggtggg                                                             >  1:471587/1‑244 (MQ=255)
                                                                                                                                                                                 atatTAAAGACTATCTGGCAATTCCCAATGTGGAGGCATGTGGC‑GGTTA‑TTTGTTTTT‑AGAAAAAAAAA‑TTTTTAATG‑GAAAAAACTGGCA‑GGAAATAGGTAG‑GGTTAA‑‑CAAAGTGGAGGC‑GGGATGGGTCAAACCTTAATCATAAATTAATCAATAAACTCAACCGCACTTCAAAATCAATGATAAAGTTCGGGGGTTTTTTGTTGGGTTTTTATAAACCGCTATTATTTTGCTTCTTTATAATGCTgg                                                              >  1:637915/1‑250 (MQ=255)
                                                                                                                                                                                   atTAAAGACTATCTGGCAATTCCCAATGTGGTGGCATGTGGC‑GGTTC‑TTTGTTTTT‑AGAAAAGAAAC‑TTATCAATG‑AAAAAAGCTGGCC‑GGAAATCGGG‑C‑GGGTAA‑CCAAAAAGGAGGC‑GGAATGGGTCAACACTTAATCATGAATTAATCAATAAACCCAACCGCACTTCAACAACAATGTTAAAGTGCGGTGGTTTTTCGTTTCGTTTTTATAAAGCGCTATTATTTTGCTGCTTTATAAAGCTGGCGa                                                           >  1:143776/1‑251 (MQ=255)
                                                                                                                                                                                   atTAAAGACTATCTGGCAATTCCCAATGTGGTGGCATGTGGC‑GGTTC‑TTTGTTTGT‑AGAAAAAAAAA‑TTATCAATG‑AAAAAAGCTGGCA‑GGAAATAAGT‑C‑GGGTAA‑ACAAAAAGGGGGT‑GGCATGGATTAACAATTAATCATGAAATAAACAATAAACTCAACCGCACTTCAACAACAAAGTTAAAGTGGGGGGGGTTTTCTTTGCGTTTTTATAACGCGCCATTTTTTTGCTGCTTTATAAAGCTTGCgg                                                           >  1:641534/1‑250 (MQ=255)
                                                                                                                                                                                   atTAAAGACTATCTGGCAATTCCCAATGTGGTGGCATGTGGC‑GGTTC‑TTGGGTTGT‑AGAAAAGAAAA‑TTATCAATG‑AAAAAAGCTGGCA‑GGAAATCGGT‑C‑GGTTAA‑CCAAAAAGGGGGC‑GGGATGGATCAACACTTAATCATGAATTAATCAATAAACTCAACCGCACTTCAACATCAATGTTTAAGTGCGGGGGTTTTTCGTTGCGTTTTTTTAAAGCGCTATTATTTTGCTGCTTTTTAAGGCTGGCgg                                                           >  1:126083/1‑250 (MQ=255)
                                                                                                                                                                                                 ggCAATTCCCAATGTGGTGGCATGTGGC‑GGTTC‑TTGGTTTGT‑AGAAAAGAAAC‑TTATCAATG‑AAAAAAGCTGGCA‑GGAAAACGGT‑C‑GGTTAA‑CCAAAGAGGGGGC‑GGAATGGATCAACACTTAATCATGAATTAATCAATAAACTCAACCGCACTTCAACATCAATg                                                                                                                                 >  1:405636/1‑167 (MQ=255)
                                                                                                                                                                                                     aTTCCCAATGTGGTGGCATGTGGC‑GGTTC‑TTGGTTTGT‑AGAAAAGAAAC‑TTATCAATG‑AAAAAAGCTGGCA‑GGAAATCGGT‑C‑GGTTAA‑CCAAAGAGGTGGC‑GGAATGGATCAACACTTAATCATGAATTAATCAATAAACTCAACCGCACTTCAACATCAATGTTAAAGTGCGGTGGTTTTTCGTTGCGTTTTTATAACGCGCTATTATTTTGCTGCTTTATAAGGCTGGCGATAGAATTTCAAATAATAg                                         <  1:438999/251‑1 (MQ=255)
                                                                                                                                                                                                      ttCCCAATGTGGTGGCATGTGGC‑GGTTC‑TTGGTTTGT‑AGAAAAAAAAC‑TTATCAATG‑AAAAAAGCTGGCA‑GGGAATAGGT‑C‑GGTTAA‑CCAAAGAGGGGGC‑GGAATGGATCAACACTTAATCATGAATTAATCAATAAAATCAACCGCACTTCAACATCAATGTTAAAATGCGGTGGTTTTTCGTTGCGTTTTTATAACGCGCTATTATTTTTCTGCTTTATAAGGCTGGCGATAGAATTTCAAATAATAGt                                        >  1:66195/1‑251 (MQ=255)
                                                                                                                                                                                                                       tgtgGC‑GGTTC‑TTGGTTTGT‑AGAAAAGAAAC‑TTATCAATG‑AAAAAAGCTGGCA‑GGAAATCGGT‑C‑GGTTAA‑CCAAAGAGGTGGC‑GGAATGGATCAACACTTAATCATGAATTAATCAATAAACTCAACCGCACTTCAACATCAATGTTAAAGTGCGGTGGTTTTTCGTTGCGTTTTTATAACGCGCTATTATTTTGCTGCTTTATAAGGCTGGCGATAGAATTTCAAATAATAGTCGTTAACCATTTTATCt                       <  1:322718/251‑1 (MQ=255)
                                                                                                                                                                                                                               gTTC‑TTGGTTTGT‑AGAAAAGAAAC‑TTATCAATG‑AAAAAAGCTGGCA‑GGAAATCGGT‑C‑GGTTAA‑CCAAAGAGGTGGC‑GGAATGTATCAACACTTAATCATGAATTAATCAATAAACTCAACCGCACTTCAACATCAATGTTAAAGTGCGGTGGTTTTTCGTTGCGTTTTTATAACGCGCTATTATTTTGCTGCTTTATAAGGCTGGCGATAGAAt                                                     <  1:338467/214‑1 (MQ=255)
                                                                                                                                                                                                                                  c‑TTGGTTTGT‑AGAAAAGAAAC‑TTATCAATG‑AAAAAAGCTGGCA‑GGAAATCGGT‑C‑GGTTAA‑CCAAAGAGGTGGC‑GGAATGGATCAACACTTAATCATGAATTAATCAATAAACTCAACCGCACTTCAACATCAATGTTAAAGTGCGGTGGTTTTTCGTTGCGTTTTTATAACGCGCTATTATTTTGCTGCTTTATAAGGCTGGCGATAGAATTTCAAATAATAGTCGTTAACCATTTTATCTAAATCCACAt             <  1:575313/251‑1 (MQ=255)
                                                                                                                                                                                                                                      ggTTTGT‑AGAAAAGAAAC‑TTATCAATG‑AAAAAAGCTGGCA‑GGAAATCGGT‑C‑GGTTAA‑CCAAAGAGGTGGC‑GGAATGGATCAACACTTAATCATGAATTAATCAATAAACTCAACCGCACTTCAACATCAATGTTAAAGTGCGGTGGTTTTTCGTTGCGTTTTTATAACGCGCTATTATTTTGCTGCTTTATAAGGCTGGCGATAGAATTTCAAATAATAGTCGTTAACCATTTTATCTAAATCCACATcg           >  1:206490/1‑250 (MQ=255)
                                                                                                                                                                                                                                                aaaagaaaC‑TTATCAATG‑AAAAAAGCTGGCA‑GGAAATCGGT‑C‑GGTTAA‑CCAAAGAGGTGGC‑GGAATGGATCAACACTTAATCATGAATTAATCAATAAACTCAACCGCACTTCAACATCAATGTTAAAGTGCGGTGGTTTTTCGTTGCGTTTTTATAACGCGCTATTATTTTGCTGCTTTATAAGGCTGGCGATAGAATTTCAAATAATAGTCGTTAACCATTTTATCTAAATCCACATCGCGAAAAAgc  <  1:452545/250‑1 (MQ=255)
                                                                                                                                                                                                                                                aaaagaaaC‑TTATCAATG‑AAAAAAGCTGGCA‑GGAAATCGGT‑C‑GGTTAA‑CCAAAGAGGTGGC‑GGAATGGATCAACACTTAATCATGAATTAATCAATAAACTCAACCGCACTTCAACATCAATGTTAAAGTGCGGTGGTTTTTCGTTGCGTTTTTATAACGCGCTATTATTTTGCTGCTTTATAAGGCTGGCGATAGAATTTCAAATAATAGTCGTTAACCATTTTATCTAAATCCACATCGCGAAAAAgc  <  1:201848/250‑1 (MQ=255)
                                                                                                                                                                                                                                                aaaagaaaC‑TTATCAATG‑AAAAAAGCTGGCA‑GGAAATCGGT‑C‑GGTTAA‑CCAAAGAGGTGGC‑GGAATGGATCAACACTTAATCATGAATTAATCAATAAACTCAACCGCACTTCAACATCAATGTTAAAGTGCGGTGGTTTTTCGTTGCGTTTTTATAACGCGCTATTATTTTGCTGCTTTATAAGGCTGGCGATAGAATTTCAAATAATAGTCGTTAACCATTTTATCTAAATCCACATCGCGAAAAAgc  <  1:489115/250‑1 (MQ=255)
                                                                                                                                                                                                                                                aaaagaaaC‑TTATCAATG‑AAAAAAGCTGGCA‑GGAAATCGGT‑C‑GGTTAA‑CCAAAGAGGTGGC‑GGAATGGATCAACACTTAATCATGAATTAATCAATAAACTCAACCGCACTTCAACATCAATGTTAAAGTGCGGTGGTTTTTCGTTGCGTTTTTATAACGCGCTATTATTTTGCTGCTTTATAAGGCTGGCGATAGAATTTCAAATAATAGTCGTTAACCATTTTATCTAAATCCACATCGCGAAAAAgc  <  1:487032/250‑1 (MQ=255)
                                                                                                                                                                                                                                                     aaaC‑TTATCAATG‑AAAAAAGCTGGCA‑GGAAATCGGT‑C‑GGTTAA‑CCAAAGAGGTGGC‑GGAATGGATCAACACTTAATCATGAATTAATCAATAAACTCAACCGCACTTCAACATCAATGTTAAAGTGCGGTGGTTTTTCGTTGCGTTTTTATAACGCGCTATTATTTTGCTGCTTTATAAGGCTGGCGATAGAATTTCAAATAATAGTCGTTAACCATTTTATCTAAATCCACATCGCGaaaa     <  1:574724/242‑1 (MQ=255)
                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                        
TGCCCATCACACCGGGGGTAAACAATCCGATGGCAATTGAAGCCGCGTTGGACGTCGGTATTGAAACCGTGAAATTCTTCCCGGCGGAAGCCTCTGGTGGCGTGAAAATGATTAAAGCCTTGTTAGGCCCTTACGCACAGTTGCAAATTATGCCGACCGGCGGTATCGGTATGCAGAATATTAAAGACTATCTGGCAATTCCCAATGTGGTGGCATGTGGC‑GGTTC‑TTGGTTTGT‑AGAAAAGAAAC‑TTATCAATG‑AAAAAAGCTGGCA‑GGAAATCGGT‑C‑GGTTAA‑CCAAAGAGGTGGC‑GGAATGGATCAACACTTAATCATGAATTAATCAATAAACTCAACCGCACTTCAACATCAATGTTAAAGTGCGGTGGTTTTTCGTTGCGTTTTTATAACGCGCTATTATTTTGCTGCTTTATAAGGCTGGCGATAGAATTTCAAATAATAGTCGTTAACCATTTTATCTAAATCCACATCGCGAAAAAGC  >  1/1073516‑1074002

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑