| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | 1 | 246,776 | 0 | A | C | 42.1% | 91.0 / 17.0 | 58 | H30P (CAC→CCC) | unknown | tRNA‑dihydrouridine(20/20a) synthase (EC 1.3.1.91) |
| Reads supporting (aligned to +/- strand): ref base A (31/2); new base C (5/19); total (37/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.07e-08 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCTGCCAGCCCGTGCTGATTTGGCTATTGATTTGCTGCCTGATTTCCTCCATTATTTCACGCCAATTTAATCCGAAGCCGTTCTATCATTTTGCGGCGGGGCGCTTCCGCCATCGGATTC‑AAGAAGAAATGCAACAAGACATGAAGGACAACTCGTAAACATGCCTCAAAACCCACCGCAC‑TTTTATCGCGGACGCTTCGCCGTGGCACCCATGTTGGACTGGACGACGCGCCATTGCC‑GTTATTTCCACCGCCAATTTTCCCGCCATGCCTTGCTTTACACGGAAATGGTGACGACACAAGCCATCATCCACGCCAAATATGATCATCTCGATTTTGACCCGGCGGAACAGCCTGTGGCGTTACAACTGGGCGGCAGCGACCCTGCCCAACTCAAACATTGCGCCCAATTGGCGGAACAACGGGGCTATCAGGAAATTAACCTGAACGTGGGCTGCCCGTCCGATCGCGTGCAAAAC > 1/246528‑247005 | gCTGCCAGCCCGTGCTGATTTGGCTATTGATTTGCTGCCTGATTTCCTCCATTATTTCACGCCAATTTAATCCGAAGCCGTTCTATCATTTTGCGGCGGGGCGCTTCCGCCATCGGATTC‑AAGAAGAAATGCAACAAGACATGAAGGACAACTCGTAAACATGCCTCAAAACCCACCGCAC‑TTTTATCGCGGACGCTTCGCCGTGGCACCCATGTTGGACTGGACGACGCGCCATTGCC‑GTTATTTCCAc < 1:27502/250‑1 (MQ=255) ttGCTGCTTGTTTCCCCCCATTTTTTCACCCAAATTTAACCCGAGGCCGTTCTTTCATTTTGCGGCGGGGCCCTCCCGCCATCGGATTC‑AAAAAGAAATGCAACAAAACATGAAGGACAACTCAAAAACTTGCCTCAAACCCCCCCGCTT‑TTTTATCGGGGACGCTTCGCCGTGGCCCCCATGTTGGACTGGACGCCGCTCCTTTGCC‑GTTATTTCCCCCGCCAATTTTCCCGCCATGCCTTGCTTTacac < 1:443675/251‑1 (MQ=255) gcCTGATTTCCTCCATTATTTCACGCCAATTTAATCCGAAGCCGTTCTATCATTTTGCGGCGGGGCGCTTCCGCCCTCGGCTTC‑TAGAAGAAATGCAACAAAAAATGAAGGACAAACCGTAAACATGCCTCAAAACCCACCCCAC‑CTTTTTCTAGGACGCTTCCCCGTGGCACCCACGTTTGTCTGGGCGAACCGCCCTTGCC‑GCTATTTCCCACGCCCATTTTTCCCCCACGCCCTGCTTTTCACGGaaa > 1:195712/1‑251 (MQ=255) cTGATTTCCTCCATTATTTCACGCCAATTTAATCCGAAGCCGTTCTATCATTTTGCGGCGGGGCGCTTCCGCCATCGGATTT‑AAGAAGAAATGCAACAAGACATGAAGGACAACTCGTAAACATGCCTCAAAACCCACCGCAC‑TTTTATCGCGGACGCTTCGCCCTGGCACCCATGTTGGACTGGACGACGCGCCATTGCC‑GTTATTTCCACCGCCAATTTTCCCGCCATGCCTTGCTTTACACGGAAATg > 1:465186/1‑251 (MQ=255) gATTTCCTCCATTATTTCACGCCAATTTAATCCGAAGCCGTTCTATCATTTTGCGGCGGGGCGCCTCCCCCCCCGGGTTA‑AAGAAAAAAAACAACCACACATAAAAGACAACAAGCCAACACGCCCCAAAACCCACCCCCA‑TTTTATCTCGGGCGCGTCGCCCTGGCGCCCATGTTGGACTGGACGGCGCCCCCTGTCC‑GGTATTTCCCCCGCCAACTTTTCCGCCATTCCCTGCTTTacacccgtctctt > 1:281520/1‑242 (MQ=255) ccATTATTTCACGCCAATTTAATCCGAAGCCGTTCTATCATTTTGCGGCGGGGGGCTTCCCCCCTCGGGTTT‑AAGAAGAAAAGCAACAAGACATGAAGGACAACTCGTAAAAATGCCTCCAAAACCACCCCAC‑TTTTATCGCGGGCGCTTCGCCGCGGCACCCCTGTTGGACTGGACGACGCGCCATTGCC‑GTTATTTCCACCCCCCATTTTTCCCCCCTGCCCTGCTTTTCACCGGAATGGGGACGaca > 1:426070/1‑250 (MQ=255) attTCACGCCAATTTAATCCGAAGCCGTTCTATCATTTTGCGGCGGGGCGCTTCCGCCATCGGGTTC‑AAGAAGAAATGCAACAAGACATGAAGGACAAATCGTAAACAAGCCTCAAAAACCACCGCCC‑TTTTTTTGCGGACGCTTCGCCGTGGCACCCACGTTGGACTGGGCGAAGCGCCCTTGCC‑GTTATTTCCCCCGCCCATTTTTCCGCCATGCCTTGCTTTACACGGAAATGGTGACGACACaaac > 1:62355/1‑248 (MQ=255) attTCACGCCAATTTAATCCGAAGCCGTTCTATCATTTTGCGGCGGGGCGCCTCCGCCATCGGAATC‑AAGAAGAAATTCAACAAAACAAGAAAGACAACTCCTAAACATGCCTCAAAAACCACCCCAC‑CTTTATTGCGGACGCCTCGCCGTGGCACCCCTGCTGGACCGGACGACGCGCCATTGCCGGTTTTTTCC‑CCCCCAAATTTCCCCCCACCCCCTGCTTTTAACGGAAATGGTGGCGACACaaac > 1:603992/1‑248 (MQ=255) cGCCAATTTAATCCGAAGCCGTTCTATCATTTTGCGGCGGGGCGCCTCCCCCCTCGGATTC‑AAGAAGAAAAGCAACAAAACAAGAAGGACAAATCGTAAAAAAGCCTCAAAAACCACCGCCC‑TTTTTTCGCGGACGCTTCGCCCTGGCACCCCTGTTGGGCTGGACGACGCGCCCTTGCC‑GTTATTTCCACCCCCCATTTTTCCGCCCCTCCCTGCTTTTCACGGGAAAGGGGACGACACAAGccctcct > 1:131937/1‑245 (MQ=255) cccattttAACCCGAAGCCGTTTTATCATTTGGCGGGGGGGCGCTTCCGCCATGGGATTC‑AAGAAAAAATGCAACAAGACATGAAGGAAAACTCGTAAACATGCCTCAAACCCCCCCGCAT‑TTTTACCGCGGACGCTTCGCCGTGGCCCCCATGTTGGACGGGACGCCGCGCCTTTGCC‑GTTATTCCCCCCGCCAATTTTCCCGCCATGCCTTGCTTTACACGGAAATGGTGACGACACAAGCCATCATcc < 1:316669/246‑1 (MQ=255) ttttACTCCGAAGCCTTTTTATCTTTTTGGGGGGGGGCGCTCCCGCCAT‑GGAATCAAAGAAAAAATGCAACAAGAAATGAAGGACACCTCGTAAACATGCCTCAAACCCCCCCGCTT‑TTTTACCGCGGCCGCTTCGCGGTGGCCCCCATGTGGGACGGGACGACGCCCCTTTGCC‑GTTTTTCCCCCCGCAAATTTCCCCGCCATGCCTCGCTTTACACGGAAATGGTGACGACACAAGCCATCATCCACGc < 1:590392/250‑1 (MQ=255) tttAACCCGAAGCCTTTCTATCTTTTGGCGGGGGGGCGCTTCCGCCATGGAATCC‑AAGAAGAAATGCAACAAGACATGAAGGACACCTCTTAAACATGCCTCAAACCCCCCCGCCC‑TTTTACCGCGGCCGCTTCGCCGTGGCACCCTTTTTGGACTGGACGCCGCGCCATTGCC‑TTTATTCCCCCCGCCAATTTTCCCGCCATGCCTTGCTTTACACGGAAATGGTGACGACACAAGCCATCATCCACGcc < 1:391856/251‑1 (MQ=255) ccccaaaGCCGTTCAATCTTTTTGCGGCGGGGCTCTTCCGCCATCGGATTA‑AAAAAAAAATGCAACAAGAAATAAAGGACACCTCGTAAACAT‑CCCAAAAACCCCCCCCCCTTTTTCTCGCGGACGCTGCGCCGGGGCCCCCATGTTGGACTGGACGCCGCGCTATTGCC‑GTTTTTTCCCCCGCCAATTTTCCCGCCATGCCTTGCTTTACACGGAAATGGTGACGACACAAGCCATCATCCACGCCAAat < 1:643281/246‑1 (MQ=255) aaGCCGTTCTATCATTTTGCGGCGGGGCGCTTCCGCCCTCGGGTTC‑AAGAAGAAATGCAACAAAACATGAAGGAAAACTCGTAAACATGCCTCAAAAACCACCCCCC‑TTTTTTCGCGGACGCTTCGCCGGGGCACCCACGTTGGACTGGACGACGCGCCCCTGCC‑GGTATTTCCCCCGCCAATTTTCCCGCCATGCCTTGCTTTTCACGGAAATGGTGGCGACACAAGCCACCATccccccccaaaatgat > 1:298691/1‑237 (MQ=255) ccGTTCTATCATTTGGCGGCGGGGCGCTCCCGCCATCGAATCA‑AAAAAGAAATGCAACAAGACATGAAGGACAACTCGTAACCATGCCTCAAACCCCCCCGCAC‑TTTTATCGCGGCCGCTTCGCCGTGGCCCCCATGTTGGACTGGACGCCGCGCCTTGGCC‑GTTATTTCCACCGCCAATTTTCCCGCCATGCCTTGCTTTACACGGAAATGGTGACGACACAAGCCATCATCCACGCCAAATATGatcat < 1:384844/251‑1 (MQ=255) tttCTATCATTTGGGGGCGGGGCGCTCCCGCCATCGGATCA‑AAAAAAAAATGCAACAAGACAGGAAGGACAACTCGTAAACATGCCTCAAACCCCCCCGCAT‑TTTTATCGCGGCCGCTCCGCCGTGGCCCCCATGTTGGACGGGACGCGGCGCCATTGCC‑TTTATTTCCCCCGCAAATTTTCCCGCCATGCCTTGCTTTACACGGAAATGGTGACGACACAAGCCATCATCCACGCCAAATATGat < 1:453222/245‑1 (MQ=255) gTTCTATCATTTTGCGGCGGGGCGCTTCCGCCCTCGGATTC‑AAGAAGAAATGCAACAAGAAAAGAAAGACAACTCGTAAAAAAGCCTCAAAAACCACCGCAC‑TTTTTTCGCGGGCGCCTCGCCGGGGCACCCCTGTTGGACTGGGCGACGCGCCATTGCC‑GTTATTTTCCCCGCCCATTTTTCCCCCCTGCCTTGCTTTTCACGGAAATGGGGACGACACAAACCATCATCCACGCCAAAAATGatcat > 1:178179/1‑249 (MQ=255) gTTCTATCATTTTGCGGCGGGGCGCTTCCGCCATCGGATTC‑AAGAAAAAAAGCAACAAAACAAGAAGGGCAACTCGTAAACAAGCCTCAAAAACCCCCCCCC‑TTTTATTTCGGACGCTTCGCCCTGGGACCCCTGTTGGGCTGGACGACGCGCCCTTGCC‑GGTATTTTCACCCCCCATTTTCCCCCCATGCCTTGCTTTACCCGGGAAAGGTGGCGACACAAACCACCATccccccccaaaaagatcatc > 1:197959/1‑232 (MQ=255) tcttttggggggggggCGCTCCCCCCATGGAATTC‑AAAAAAAAATGAAACAAGACAGAAAGGACAACTCGAAAACATGCCTAAAACCCCCCCGCAC‑TTTTATCGCGGCCGCTTCGCGGTGGCCCCCATTTTGGACGGGACGCCGCGCCTTTGCC‑GTTATTCCCCCCGCAAATTTTCCCGCCATGCCTTGCTTTACACGGAAATGGTGACGACACAAGCCATCATCCACGCCAAATATGATCATCTCGAttt < 1:388265/239‑1 (MQ=255) cgcTCCCGCCATGGGATTA‑AAAAAAAAATGCAACAAGACATGAAGGACAACTCGTAAACATGCCTCAAACCCCCCCGCAT‑TTTTACCGCGGACGCTCCGCCGTGGCCCCCATGTTGGACTGGCCGACGCGCCATTGCC‑GTTATTCCCCCCGCCATTTTTCCCGCCATGCCTTGCTTTACACGGAAATGGTGACGACACAAGCCATCATCCACGCCAAATATGATCATCTCGATTTTGACCCGGCGGAACAg < 1:641356/251‑1 (MQ=255) ctcccgccgTCGGATTA‑AAGAAAAAATGCAACAAGAAATGAGGGACACCCCGTAAACAGGCCTCAAACCCCCCCGCCC‑TTTTACCGCGGACGCTCCGCCGTGGCCCCCTTGTTGGACTGGACGCCGCCCCATTGCC‑TTTATTTCCCCCCCCAATTTTCCCGCCATGCCTTGCTTTACACGGAAATGGTGACGACACAAGCCATCATCCACGCCAAATATGATCATCTCGATTTTGACCCGGCGGAACAGcc < 1:185367/248‑1 (MQ=255) ataaaaaaaaaaaTGCAACAAGAAATGAAGGACACCCCGTAAACATGCCAAAAACCCCCCCCCCC‑TTTTACCGCGGCCGCTCCGCCGTGGCCCCCATGTTGGCCTGGACGCCGCGCCTTTGCC‑GTTATTCCCCCCGCCAATTTTCCCGCCATGCCTTGCTTTACACGGAAATGGTGACGACACAAGCCATCATCCACGCCAAATATGATCATCTCGATTTTGACCCGGCGGAACAGCCTGTGGCGTTACaa < 1:343630/241‑1 (MQ=255) tccaaaaagaaATACAACAAGACAAAAAGGACACCCCGAAAACATGCCTCAAACCCCCCCCCCT‑TTTTATCGCGGCCCCTCCGCCGTGGCCCCCTTTTTGGACTGGCCCCCGCGCCTTTCCC‑GTTATTCCCCCCGCCAATTTTCCCGCCATGCCTTGCTTTACACGGAAATGGTGACGACACAAGCCATCATCCACGCCAAATATGATCATCTCGATTTTGACCCGGCGGAACAGCCTGTGGCGTTACAAc < 1:604442/245‑1 (MQ=255) tC‑AAGAAGAAATGCAACAAGACATGAAGGACAACTCGTAAACATGCCTCAAAACCCACCGCAC‑TTTTATCGCGGACGCTTCGCCGTGGCACCCATGTTGGACTGGACGACGCGCCATTGCC‑GTTATTTCCACCGCCAATTTTCCCGCCATGCCTTGCTTTACACGGAAATGGTGACGACACAAGCCATCATCCACGCCAAATATGat > 1:399650/1‑207 (MQ=255) aaTGCAACAAGACATGAAGGACAACTCGTAAACATGCCTCAAAACCCACCGCAC‑TTTTATCGCGGACGCTTCGCCGTGGCACCCATGTTGGACTGGACGACGCGCCATTGCC‑GTTATTTCCACCGCCAATTTTCCCGCCATGCCTTGCTTTACACGGAAATGGTGACGACACAAGCCATCATCCACGCCAAATATGATCATCTCGATTTTGACCCGGCGGAACAGCCTGTGGCGTTACAACTGGGCGGCAg > 1:477542/1‑251 (MQ=255) caacaaGACATGAAGGACAACTCGTAAACATGCCTCAAAACCCACCGCAC‑TTTTATCGCGGACGCTTCGCCGTGGCACCCATGTTGGACTGGACGACGCGCCATTGCC‑GTTATTTCCACCGCCAATTTTCCCGCCATGCCTTGCTTTACACGGAAATGGTGACGACACAAGcc > 1:157422/1‑173 (MQ=255) gACATGAAGGACAACTCGTAAACATGCCTCAAAACCCACCGCAC‑TTTTATCGCGGACGCTTCGCCGTGGCACCCATGTTGGACTGGACGACGCGCCATTGCC‑GTTATTTCCACCGCCAATTTTCCCGCCATGCCTTGCTTTACACGGAAATGGTGACGACACAAGCCATCATCCACGCCAAATATGat > 1:38042/1‑188 (MQ=255) aCATGAAGAAAAACTCGTAAACATCCCTCAAACCCCCCCGCAT‑TTTTACCGCGGACCCTCCGCCGTGGCCCCCATGTTGGACGGGACGCCGCGCCATTGCC‑GTTATTCCCCCCGCCAATTTTCCCGCCATGCCTTGCTTTACACGGAAATGGTGACGACACAAGCCATCATCCACGCCAAATATGATCATCTCGATTTTGACCCGGCGGAACAGCCTGTGGCGTTACAACTGGGCGGCAGCGACCCTGccc < 1:455958/251‑1 (MQ=255) aTGAAGGACAACTCGTAAACATGCCTCAAAACCCACCGCAC‑TTTTATCGCGGACGCTTCGCCGTGGCACCCATGTTGGACTGGACGACGCGCCATTGCC‑GTTATTTCCACCGCCAATTTTcc > 1:150772/1‑122 (MQ=255) aTGAAGGACAACTCGTAAACATGCCTCAAAACCCACCGCAC‑TTTTATCGCGGACGCTTCGCCGTGGCACCCATGTTGGACTGGACGACGCGCCATTGCC‑GTTATTTCCACCGCCAATTTTCCCGCCATGCCTTGCTTTACACGGAAATGGTGACGACACAAGCCATCATCCACGCCAAATATGATCATCTCGATTTTGACCCGGCGGAACAGCCTGTGGCGTTACAACTGGGCGGCAGCGAcc > 1:481749/1‑243 (MQ=255) aTGAAGGACAACTCGTAAACATGCCTCAAAACCCACCGCAC‑TTTTATCGCGGACGCTTCGCCGTGGCACCCATGTTGGACTGGACGACGCGCCATTGCC‑GTTATTTCCACCGCCAATTTTCCCGCCATGCCTTGCTTTACACGGAAATGGTGACGACACAAGCCATCATCCACGCCAAATATGATCATCTCGATTTTGACCCGGCGGAACAGCCTGTGGCGTTACAACTGGGCGGCAGCGACCCTGCCCaa > 1:537473/1‑251 (MQ=255) gAAGGACAACTCGTAAACATGCCTCAAAACCCACCGCAC‑TTTTATCGCGGACGCTTCGCCGTGGCACCCATGTTGGACTGGACGACGCGCCATTGCC‑GTTATTTCCACCGCCAATTTTCCCGCCATGCCTTGCTTTACACGGAAATGGTGACGACACAAGCCATCATCCACGCCAAATATGATCATCTCg > 1:570211/1‑190 (MQ=255) aaGGACAACTCGTAAACATGCCTCAAAACCCACCACAC‑TTTTATCGCGGACGCTTCGCCGTGGCACCCATGTTGGACTGGACGACGCGCCATTGCC‑GTTATTTCCACCGCCAATTTTCCCGCCATGCCTTGCTTTACACGGAAATGGTGACGACACAAGCCATCATCCACGCCAAATATGATCATCTCGATTTTGACCCGGCGGAACAGCCTGTGGCGTTACAACTGGGCGGCAGCGACCCTGCCCAACt > 1:59362/1‑250 (MQ=255) aaaccTCGAAAACATGCCTCAAACCCCCCCGCCT‑TTTTATCGCGGACGCTCCGCCGTGGCCCCCATGTTGGACGGGACGCCGCCCCATTGCC‑GTTATTCCCCCCGCCAATTTTCCCGCCATGCCTTGCTTTACACGGAAATGGTGACGACACAAGCCATCATCCACGCCAAATATGATCATCTCGATTTTGACCCGGCGGAACAGCCTGTGGCGTTACAACTGGGCGGCAGCGACCCTGCCCAACTCAAAc < 1:609979/247‑1 (MQ=255) cTCGTAAACATGCCTCAAAACCCACCGCAC‑TTTTATCGCGGACGCTTCGCCGTGGCACCCATGTTGGACTGGACGACGCGCCATTGCC‑GTTATTTCCACCGCCAATTTTCCCGCCATGCCTTGCTTTACACGGAAATGGTGACGACACAAGCCATCATCCACGCCAAATATGATCATCTCGATTTTGACCCGGCGGAACAGCCTGTGGCGTTACAACTGGGCGGCAGCGACCCTGCCCAACTCAAACAt > 1:314222/1‑249 (MQ=255) cTCGTAAACATGCCTCAAAACCCACCGCAC‑TTTTATCGCGGACGCTTCGCCGTGGCACCCATGTTGGACTGGACGACGCGCCATTGCC‑GTTATTTCCACCGCCAATTTTCCCGCCATGCCTTGCTTTACACGGAAATGGTGACGACACAAGCCATCATCCACGCCAAATATGATCATCTCGATTTTGACCCGGCGGAACAGCCTGTGGCGTTACAACTGGGCGGCAGCGACCCTGCCCAACTCAAACAt > 1:481350/1‑249 (MQ=255) cTCGTAAACATGCCTCAAAACCCACCGCAC‑TTTTATCGCGGACGCTTCGCCGTGGCACCCATGTTGGACTGGACGACGCGCCATTGCC‑GTTATTTCCACCGCCAATTTTCCCGCCATGCCTTGCTTTACACGGAAATGGTGACGACACAAGCCATCATCCACGCCAAATATGATCATCTCGATTTTGACCCGGCGGAACAGCCTGTGGCGTTACAACTGGGCGGCAGCGACCCTGCCCAACTCAAACATTg > 1:33754/1‑251 (MQ=255) cTCGTAAACATGCCTCAAAACCCACCGCAC‑TTTTATCGCGGACGCTTCGCCGTGGCACCCATGTTGGACTGGACGACGCGCCATTGCC‑GTTATTTCCACCGCCAATTTTCCCGCCATGCCTTGCTTTACACGGAAATGGTGACGACACAAGCCATCATCCACGCCAAATATGATCATCTCGATTTTGACCCGGCGGAACAGCCTGTGGCGTTACAACTGGGCGGCAGCGACCCTGCCCAACTCAAACATTg > 1:242891/1‑251 (MQ=255) cTCGTAAACATGCCTCAAAACCCACCGCAC‑TTTTATCGCGGACGCTTCGCCGTGGCACCCATGTTGGACTGGACGACGCGCCATTGCC‑GTTATTTCCACCGCCAATTTTCCCGCCATGCCTTGCTTTACACGGAAATGGTGACGACACAAGCCATCATCCACGCCAAATATGATCATCTCGATTTTGACCCGGCGGAACAGCCTGTGGCGTTACAACTGGGCGGCAGCGACCCTGCCCAACTCAAACATTg > 1:349859/1‑251 (MQ=255) cTCGTAAACATGCCTCAAAACCCACCGCAC‑TTTTATCGCGGACGCTTCGCCGTGGCACCCATGTTGGACTGGACGACGCGCCATTGCC‑GTTATTTCCACCGCCAATTTTCCCGCCATGCCTTGCTTTACACGGAAATGGTGACGACACAAGCCATCATCCACGCCAAATATGATCATCTCGATTTTGACCCGGCGGAACAGCCTGTGGCGTTACAACTGGGCGGCAGCGACCCTGCCCAACTCAAACATTg > 1:508943/1‑251 (MQ=255) cGTAAACATGCCTCAAAACCCACCGCAC‑TTTTATCGCGGACGCTTCGCCGTGGCACCCATGTTGGACTGGACGACGCGCCATTGCC‑GTTATTTCCACCGCCAATTTTCCCGCCATGCCTTGCtt > 1:44914/1‑124 (MQ=255) gCCTCAAAACCCACCGCAC‑TTTTATCGCGGACGCTTCGCCGTGGCACCCATGTTGGACTGGACGACGCGCCATTGCC‑GTTATTTCCACCGCCAATTTTCCCGCCATGCCTTGCTTTACACGGAAATGGTGACGACACAAGCCATCATCCACGCCAAATATGATCATCTCGATTTTGACCCGGCGGAACAGCCTGTGGCGTTACAACTGGGCGGCAGCGACCCTGCCCAACTCAAACATTGCGCCCAATTgg > 1:599857/1‑251 (MQ=255) ccGCAC‑TTTTATCGCGGACGCTTCGCCGTGGCACCCATGTTGGACTGGACGACGCGCCATTGCC‑GTTATTTCCACCGCCAATTTTCCCGCCATGCCTTGCTTTACACGGAAATGGTGACGACACAAGCCATCATCCACGCCAAATATGat > 1:202935/1‑150 (MQ=255) cAC‑TTTTATCGCGGACGCTTCGCCGTGGCACCCATGTTGGACTGGACGACGCGCCATTGCC‑GTTATTTCCACCGCCAATTTTCCCGCCATGCCTTGCTTTACACGGAAATGGTGACGACACAAGCCATCATCCACGCCAAATATGATCATCTCGATTTTGACCCGGCGGAACAGCCTGTGGCGTTACAACTGGGCGGCAGCGACCCTGCCCAACTCAAACATTGCGCCCAATTGGCGGAACAACGGGGCTa > 1:201595/1‑251 (MQ=255) ccggggccGCTGCGCCGTGCCCCCCATGTTGGACGGGCCGCCGCCCCTTTGCC‑TTTATTCCCCCCGCCAATTTCCCCGCCATGCCTTGCTTTACACGGAAATGGTGACGACACAAGCCATCATCCACGCCAAATATGATCATCTCGATTTTGACCCGGCGGAACAGCCTGTGGCGTTACAACTGGGCGGCAGCGACCCTGCCCAACTCAAACATTGCGCCCAATTGGCGGAACAACGGGGCTATCAGGaaa < 1:63893/244‑1 (MQ=255) cctcTTCCCCGTGCCCCCCATGTGGGCCGGGACAACGCGCCTTTGCT‑TTTTTTTCCCCCGCCAATTTCCCCGCCATGCCTTGCTTTACACGGAAATGGTGACGACACAAGCCATCATCCACGCCAAATATGATCATCTCGATTTTGACCCGGCGGAACAGCCTGTGGCGTTACAACTGGGCGGCAGCGACCCTGCCCAACTCAAACATTGCGCCCAATTGGCGGAACAACGGGGCTATCAGGAAATTAAcc < 1:201366/248‑1 (MQ=255) aCGCTGCGCCGTGGCCCCCATGTTGGACGGGCCGCCGCGCCATTGCC‑GTTATTCCCCCCCCCAATTTTCCCGCCATGCCTTGCTTTACACGGAAATGGTGACGACACAAGCCATCATCCACGCCAAATATGATCATCTCGATTTTGACCCGGCGGAACAGCCTGTGGCGTTACAACTGGGCGGCAGCGACCCTGCCCAACTCAAACATTGCGCCCAATTGGCGGAACAACGGGGCTATCAGGAAATTAAcc < 1:611718/251‑1 (MQ=255) tCGCCGTGGCACCCATGTTGGACTGGACGACGCGCCATTGCC‑GTTATTTCCACCGCCAATTTTCCCGCCATGCCTTGCTTTACACGGAAATGGTGACGACACAAGCCATCATCCACGCCAAATATGATCATCTCGATTTTGACCCGGCGGAACAGCCTGTGGCGTTACAACTGGGCGGCAGCGACCCTGCCCAACTCAAACATTGCGCCCAATTGGCGGAACAACGGGGCTATCAGGAAATTAACCTGaa > 1:445430/1‑250 (MQ=255) ccGTGGCACCCATGTTGGACTGGACGACGCGCCATTGCC‑GTTATTTCCACCGCCAATTTTCCCGCCATGCCTTGCTTTACACGGAAATGGTGACGACACAAGCCATCATCCACGCCAAATATGATCATCTCTATTTTGACCCGGCGGAACAGCCTGTGGCGTTACAACTGGGCGGCAGCGACACTGCCCAACTCAAACATTGCGCCCAATTGGCGGAACAACGGGGCTATCAGGAAATTAACCTGAACGTg > 1:339397/1‑251 (MQ=255) ccGTGGCACCCATGTTGGACTGGACGACGCGCCATTGCC‑GTTATTTCCACCGCCAATTTTCCCGCCATGCCTTGCTTTACACGGAAATGGTGACGACACAAGCCATCATCCACGCCAAATATGATCATCTCGATTTTGACCCGGCGGAACAGCCTGTGGCGTTACAACTGGGCGGCAGCGACCCTGCCCAACTCAAACATTGCGCCCAATTGGCGGAACAACGGGGCTATCAGGAAATTAACCTGAACGTg > 1:573556/1‑251 (MQ=255) cATGTTGGACTGGACGACGCGCCATTGCC‑GTTATTTCCACCGCCAATTTTCCCGCCATGCCTTGCTTTACACGGAAATGGTGACGACACAAGCCATCATCCACGCCAAATATGATCATCTCGATTTTGACCCGGCGGAACAGCCTGTGGCGTTACAACTGGGCGGCAGCGACCCTGCCCAACTCAAACATTGCGCCCAATTGGCGGAACAACGGGGCTATCAGGAAATTAACCTGAACGTGGGCTGCCCGt > 1:392792/1‑251 (MQ=255) aTGTTGGACTGGACGACGCGCCATTGCC‑GTTATTTCCACCGCCAATTTTCCCGCCATGCCTTGCTTTACACGGAAATGGTGACGACACAAGCCATCATCCACGCCAAATATGATCATCTCGATTTTGACCCGGCGGAACAGCCTGTGGCGTTACAACTGGGCGGCAGCGACCCTGCCCAACTCAAACATTGCGCCCAATTGGCGGAACAACGGGGCTATCAGGAAATTAACCTGAACGTGGGCTGCCCGt > 1:335623/1‑250 (MQ=255) aTGTTGGACTGGACGACGCGCCATTGCC‑GTTATTTCCACCGCCAATTTTCCCGCCATGCCTTGCTTTACACGGAAATGGTGACGACACAAGCCATCATCCACGCCAAATATGATCATCTCGATTTTGACCCGGCGGAACAGCCTGTGGCGTTACAACTGGGCGGCAGCGACCCTGCCCAACTCAAACATTGCGCCCAATTGGCGGAACAACGGGGCTATCAGGAAATTAACCTGAACGTGGGCTGCCCGt > 1:503249/1‑250 (MQ=255) aTGTTGGACTGGACGACGCGCCATTGCC‑GTTATTTCCACCGCCAATTTTCCCGCCATGCCTTGCTTTACACGGAAATGGCGACGACACAAGCCATCATCCACGCCACATATGATCATCTCGATTTTGACCCGGCGGAACAGCCTGTGGCGTTACAACTGGGCGGCAGCGATCCTGCCCAACTCAAACATTGCGCACAATTGGCGGTACCACGGGGCTATCAGGTAATTAACCTGAACGTGGGCTGCCCgc > 1:545465/1‑249 (MQ=255) tgtgggactggacGCCGCGCCATTCCC‑GTTATTCCCCCCGCCATTTTTCCCGCCATGCCTTGCTTTACACGGAAATGGTGACGACACAAGCCATCATCCACGCCAAATATGATCATCTCGATTTTGACCCGGCGGAACAGCCTGTGGCGTTACAACTGGGCGGCAGCGACCCTGCCCAACTCAAACATTGCGCCCAATTGGCGGAACAACGGGGCTATCAGGAAATTAACCTGAACGTGGGCTGCCCGTcc < 1:106720/247‑1 (MQ=255) ggactggacGACGCGCCATTGCC‑GTTATTTCCACCGCCAATTTTCCCGCCATGCCTTGCCTTACACGGAAATGGTGACGACACAAGCCATCATCCACGCCAAATATGATCATCTCGATTTTGACCCGGCGGAACAGCCTGTGGCGTTACAACTGGGCGGCAGCGACCCTGCCCAACTCAAACATTGCGCCCAATTGGCGGAACAACGGGGCTATCAGGAAATTAACCTGAACGTGGGCCGCCCGTCCGAt > 1:430979/1‑250 (MQ=255) actggccgcccccccATTCCC‑TTTATTCCCCCCGCCAATTTTCCCGCCATGCCTTGCTTTACACGGAAATGGTGACGACACAAGCCATCATCCACGCCAAATATGATCATCTCGATTTTGACCCGGCGGAACAGCCTGTGGCGTTACAACTGGGCGGCAGCGACCCTGCCCAACTCAAACATTGCGCCCAATTGGCGGAACAACGGGGCTATCAGGAAATTAACCTGAACGTGGGCTGCCCGTCCGATcg < 1:582549/237‑1 (MQ=255) cgcgcctttccggTTATTTCCCCCGCCATTTTTCCCGCCATGCCTTGCTTTACACGGAAATGGTGACGACACAAGCCATCATCCACGCCAAATATGATCATCTCGATTTTGACCCGGCGGAACAGCCTGTGGCGTTACAACTGGGCGGCAGCGACCCTGCCCAACTCAAACATTGCGCCCAATTGGCGGAACAACGGGGCTATCAGGAAATTAACCTGAACGTGGGCTGCCCGTCCGATCGCGTGCAAAAc < 1:1837/239‑1 (MQ=255) | GCTGCCAGCCCGTGCTGATTTGGCTATTGATTTGCTGCCTGATTTCCTCCATTATTTCACGCCAATTTAATCCGAAGCCGTTCTATCATTTTGCGGCGGGGCGCTTCCGCCATCGGATTC‑AAGAAGAAATGCAACAAGACATGAAGGACAACTCGTAAACATGCCTCAAAACCCACCGCAC‑TTTTATCGCGGACGCTTCGCCGTGGCACCCATGTTGGACTGGACGACGCGCCATTGCC‑GTTATTTCCACCGCCAATTTTCCCGCCATGCCTTGCTTTACACGGAAATGGTGACGACACAAGCCATCATCCACGCCAAATATGATCATCTCGATTTTGACCCGGCGGAACAGCCTGTGGCGTTACAACTGGGCGGCAGCGACCCTGCCCAACTCAAACATTGCGCCCAATTGGCGGAACAACGGGGCTATCAGGAAATTAACCTGAACGTGGGCTGCCCGTCCGATCGCGTGCAAAAC > 1/246528‑247005 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |