| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | 1 | 1,985,101 | 0 | T | G | 40.3% | 113.9 / 14.1 | 63 | T275P (ACC→CCC) | unknown | 16S rRNA (guanine(1207)‑N(2))‑methyltransferase (EC 2.1.1.172) |
| Reads supporting (aligned to +/- strand): ref base T (8/29); new base G (25/0); total (34/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.21e-10 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TTAAATAATAAGTAGAATTCTAACTAAAAGGCTTCGCTAAGCCAGCGGAATTTCGTGCACACTTAACTCATCTGCGCACCGAATATACCTTAAACTTGTTATTTCTCGCCAGGACGTCATGACTGCCGAAATGTTGATTCAGCCAGTCGGCGTAAGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGT‑GTAAGCGGTGTCAATGCCGTCATGAAAAGGC‑GGA‑TT‑GGAAATGATCAGATCGAATTTGCCGTCAAT‑ATGGGAAAACACGTCAC‑TGGCAACCACCGTGCCTTGCAACCGATTTTCCTGTAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATC‑GGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCG > 1/1984852‑1985340 | ttAAATAATAAGTAGAATTCTAACTAAAAGGCTTCGCTAAGCCAGCGGAATTTCGTGCACACTTAACTCATCTGCGCACCGAATATACCTTAAACTTGTTATTTCTCGCCAGGACGTCATGACTGCCGAAATGTTGATTCAGCCAGTCGGCGTAAGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGT‑GTAAGCGgtg < 1:362149/251‑1 (MQ=255) gTAGAATTCTAACTAAAAGGCTTCGCTAAGCCAGCGGAATTTCGTGCACACTTAACTCATCTGCGCACCGAATATACCTTAAACTTGTTATTTCTCGCCAGGACGTCATGACTGCCGAAATGTTGATTCAGCCAGTCGGCGTAAGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGT‑GTAAGCGGGGTTAATGCCgg > 1:177612/1‑249 (MQ=255) gCTTCGCTAAGCCAGCGGAATTTCGTGCACACTTAACTCATCTGCGCACCGAATATACCTTAAACTTGTTATTTCTCGCCAGGACGTCATGACTGCCGAAATGTTGATTCAGCCAGTCGGCGTAAGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGT‑GTAAGCGGGGGTAAAGGCGTCCTGAAAAAGC‑GGA‑TT‑ggga > 1:620353/1‑249 (MQ=255) cTAAGCCAGCGGAATTTCGTGCACACTTAACTCATCTGCGCACCGAATATACCTTAAACTTGTTATTTCTCGCCAGGACGTCATGACTGCCGAAATGTTGATTCAGCCAGTCGGCGTAAGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGT‑GTAAGCGGTGTCAATGCCGTCATGAAAAGGC‑GGA‑TT‑GGAAATGATc < 1:595143/251‑1 (MQ=255) cAGCGGAATTTCGTGCACACTTAACTCATCTGCGCACCGAATATACCTTAAACTTGTTATTTCTCGCCAGGACGTCATGACTGCCGAAATGTTGATTCAGCCAGTCGGCGTAAGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGT‑GTAAGCGGGGTCAATGCCGCCATGAAAAAGC‑ggggtgggaa > 1:95336/1‑232 (MQ=255) gCGGAATTTCGTGCACACTTAACTCATCTGCGCACCGAATATACCTTAAACTTGTTATTTCTCGCCAGGACGTCATGACTGCCGAAATGTTGATTCAGCCAGTCGGCGTAAGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGT‑GTAAGCGGGGTCAATGCCGGCCTGAAAAAGC‑GGG‑TT‑GGGAATGATCAGAACGaa > 1:302061/1‑251 (MQ=255) gTGCACACTTAACTCATCTGCGCACCGAATATACCTTAAACTTGTTATTTCTCGCCAGGACGTCATGACTGCCGAAATGTTGATTCAGCCAGTCGGCGTAAGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGG‑CTAAGAGGCGGAAATGCCGGCATGCAAAGGG‑GGT‑TT‑TGAAATGAttagcgcggagttgccgtag > 1:153103/1‑232 (MQ=255) cacTTAACTCATCTGCGCACCGAATATACCTTAAACTTGTTATTTCTCGCCAGGACGTCATGACTGCCGAAATGTTGATTCAGCCAGTCGGCGTAAGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGT‑GTAAGCGGTGTCAATGCCGTCATGAAAAGGC‑GGA‑TT‑GGAAATGATCAGATc < 1:204442/233‑1 (MQ=255) cacTTAACTCATCTGCGCACCGAATATACCTTAAACTTGTTATTTCTCGCCAGGACGTCATGACTGCCGAAATGTTGATTCAGCCAGTCGGCGTAAGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGT‑GTAAGCGGGGTCAAAGCCGTCATGAAAAAGG‑GGG‑TT‑GGGAATGATCAGATcctgtctcttttaaacaat > 1:419512/1‑233 (MQ=255) cacTTAACTCATCTGCGCACCGAATATACCTTAAACTTGTTATTTCTCGCCAGGACGTCATGACTGCCGAAATGTTGATTCAGCCAGTCGGCGTAAGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGG‑GTAAGCGGGGTCAATGCCGTCATGAAAAGGG‑GGA‑TT‑GGAAAAGATCAGAACGAATTTGCCGTCAAA‑ATg > 1:499585/1‑251 (MQ=255) acTTAACTCATCTGCGCACCGAATATACCTTAAACTTGTTATTTCTCGCCAGGACGTCATGACTGCCGAAATGTTGATTCAGCCAGTCGGCGTAAGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGT‑GTAAGCGGGGTTAAAGCCGTCATGAAAAAGC‑GGA‑TT‑GGAAATGATCAGATCGAATTTGTCGTCAat‑attg > 1:447515/1‑249 (MQ=255) cTTAACTCATCTGCGCACCGAATATACCTTAAACTTGTTATTTCTCGCCAGGACGTCATGACTGCCGAAATGTTGATTCAGCCAGTCGGCGTAAGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGT‑GTAAGCGGTGTCAATGCCGTCATGAAAAGGC‑GGA‑TT‑GGaaa < 1:83623/221‑1 (MQ=255) cTTAACTCATCTGCGCACCGAATATACCTTAAACTTGTTATTTCTCGCCAGGACGTCATGACTGCCGAAATGTTGATTCAGCCAGTCGGCGTAAGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGT‑GTAAGCGGTGTCAATGCCGTCATGAAAAGGC‑GGA‑TT‑GGAAATGATCAGATCGAATTTGCCGTCAAT‑ATggg < 1:469600/251‑1 (MQ=255) cTTAACTCATCTGCGCACCGAATATACCTTAAACTTGTTATTTCTCGCCAGGACGTCATGACTGCCGAAATGTTGATTCAGCCAGTCGGCGTAAGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGT‑GTAAGCGGTGTCAATGCCGGCCTGAAAAAGC‑GGG‑TT‑GGGAAAGATCAGATCGAATTTTCCGTCAat‑attgg > 1:41337/1‑248 (MQ=255) cTTAACTCATCTGCGCACCGAATATACCTTAAACTTGTTATTTCTCGCCAGGACGTCATGACTGCCGAAATGTTGATTCAGCCAGTCGGCGTAAGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGT‑GTAAGCGGGGTCAATGCCGTCATGAAAAAGC‑GGA‑TT‑GGGAATGATTAGATCGAATTTTCCGTCAat‑attgg > 1:205387/1‑248 (MQ=255) aCTCATCTGCGCACCGAATATACCTTAAACTTGTTATTTCTCGCCAGGACGTCATGACTGCCGAAATGTTGATTCAGCCAGTCGGCGTAAGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGT‑GTAAGGGGGGTCAATGCCGTCCTGAAAAAGC‑GGG‑GT‑GGAAAAGATCAGATTGAATTTGCCGTCAAT‑ATTGGaaaa > 1:403236/1‑251 (MQ=255) aCTCATCTGCGCACCGAATATACCTTAAACTTGTTATTTCTCGCCAGGACGTCATGACTGCCGAAATGTTGATTCAGCCAGTCGGCGTAAGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGT‑GTAAGCGGGGTCAATGGCGTCATGAAAAGGCGGGA‑TG‑GGAAA‑GATTAGATCGAATTTGCCGTCAATAATGGGaaa > 1:487768/1‑251 (MQ=255) cATCTGCGCACCGAATATACCTTAAACTTGTTATTTCTCGCCAGGACGTCATGACTGCCGAAATGTTGATTCAGCCAGTCGGCGTAAGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGT‑GTAAGCGGTGTCAATGCCGTCATGAAAAGGC‑GGA‑TT‑GGAAATGATCAGATCGAATTTGCCGTCAAT‑ATGGGAAAacac < 1:629992/251‑1 (MQ=255) cATCTGCGCACCGAATATACCTTAAACTTGTTATTTCTCGCCAGGACGTCATGACTGCCGAAATGTTGATTCAGCCAGTCGGCGTAAGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGT‑GTAAGCGGGGTCCATTCCGTCATGAAAAAGC‑GGG‑TT‑GGGAATGATTAGATCGAATTTTCCGTCCAT‑ATGGGGAAaca > 1:451378/1‑250 (MQ=255) cATCTGCGCACCGAATATACCTTAAACTTGTTATTTCTCGCCAGGACGTCATGACTGCCGAAATGTTGATTCAGCCAGTCGGCGTAAGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGT‑GTAAGCGGGGTCAAAGCCGTCATGAAAAGGC‑GGG‑TT‑GGAAATGATCAGATAGAATTTTCCGTCCAT‑ATGGGaaaaaaa > 1:579333/1‑248 (MQ=255) tCTGCGCACCGAATATACCTTAAACTTGTTATTTCTCGCCAGGACGTCATGACTGCCGAAATGTTGATTCAGCCAGTCGGCGTAAGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGT‑GTAAGCGGTGTCCATGCCGTCCTGAAAAGGC‑GGA‑TT‑GGAAATTATCAGATCGAATTTTCCGTCAAA‑ATTGGAAAACACGt > 1:562230/1‑251 (MQ=255) gAATATACCTTAAACTTGTTATTTCTCGCCAGGACGTCATGACTGCCGAAATGTTGATTCAGCCAGTCGGCGTAAGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGT‑GTAAGCGGTGTCAACTCCGTCATGAAAAAGC‑GGG‑TT‑GGAAATGATCAGATCGAATTTTCCGTCAAT‑ATTGGGAAACACGTCCC‑TGGCaa > 1:344922/1‑250 (MQ=255) aaTATACCTTAAACTTGTTATTTCTCGCCAGGACGTCATGACTGCCGAAATGTTGATTCAGCCAGTCGGCGTAAGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGT‑GTAAGCGGTGTCAATGCCGTCATGAAAAGGC‑GGA‑TT‑GGAAATGATCAGATCGAATTTGCCGTCAAT‑ATGGGAAAACACGTCAC‑TGGCAacc < 1:359669/251‑1 (MQ=255) atatACCTTAAACTTGTTATTTCTCGCCAGGACGTCATGACTGCCGAAATGTTGATTCAGCCAGTCGGCGTAAGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGT‑GTAAGCGGTGTCAATGCCGCCATGAAAAAGC‑GGA‑TT‑GGGAATGGTCAGATCGAATTTGCCGTCAAT‑ATTGGaaaaaaaaacacttgcaacca > 1:578189/1‑234 (MQ=255) aCCTTAAACTTGTTATTTCTCGCCAGGACGTCATGACTGCCGAAATGTTGATTCAGCCAGTCGGCGTAAGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGT‑GTAAGCGGTGTCAATGCCGTCATGaaa < 1:335116/182‑1 (MQ=255) ccTTAAACTTGTTATTTCTCGCCAGGACGTCATGACTGCCGAAATGTTGATTCAGCCAGTCGGCGTAAGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGT‑GTAAGCGGGGTCAATGCCGTCATGAAAAGGC‑GGG‑TT‑GGAAATGATCAGATCGAATTTGCCGTCCAT‑ATGGGGAAACACGTCAC‑TGGCAACCACCgcg > 1:176572/1‑249 (MQ=255) tttCTCGCCAGGACGTCATGACTGCCGAAATGTTGATTCAGCCAGTCGGCGTAAGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGT‑GGAAAGGGGGGCAATGCCGTCCTGAAAAGGC‑GGG‑GT‑GGGAATGAACAGATCGGATTTGCCGTCAAT‑ATGGGGAAAAACGTCAC‑TGGCAAACACCGTGCCCTGCAACCCAtt > 1:550977/1‑251 (MQ=255) cGCCAGGACGTCATGACTGCCGAAATGTTGATTCAGCCAGTCGGCGTAAGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGT‑GTAAGCGGGGTCAATGCCGTCATGAAAAGGC‑GGG‑TT‑GGGAATGATCAGAACGAATTTGCCGTCAAT‑ATGGGAAAAAACGTCAC‑TGGCAACCACCCCGCCTTGCAACCGAttttt > 1:149173/1‑248 (MQ=255) ggACGTCATGACTGCCGAAATGTTGATTCAGCCAGTCGGCGTAAGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGT‑GTAAGCGGGGTCAATGCCGTCATGAAAAAGC‑GGG‑TT‑GGGAATGATCAGATCGGATTTTCCGGCCAT‑ATGGGGAAAAACGTCAC‑CGGCAACCACCGCGCCCTGCCACCGATTTTTCTGTaaa > 1:341956/1‑251 (MQ=255) gACGTCATGACTGCCGAAATGTTGATTCAGCCAGTCGGCGTAAGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGT‑GTAAGCGGTGTCAATGCCGTCATGAAAAGGC‑GGA‑TT‑GGAAATGATCAGATCGAATTTGCCGTCAAT‑ATGGGAAAACACGTCAC‑TGGCAACCACCGTGCCTTGCAACCGATTTTCCTGTaa < 1:567952/249‑1 (MQ=255) cGTCATGACTGCCGAAATGTTGATTCAGCCAGTCGGCGTAAGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGT‑GTAAGCGGGGGCAATGACGGCATGAAAAGGC‑GGA‑TT‑GGGGATGATCAGATCGAAAGTGTCGTCAAA‑ATTGGAAAACAAGTCAC‑TGGCAAACACCGGGCCTTGCAACCGATTTTTCTGTAAAGTg > 1:604373/1‑251 (MQ=255) aaTGTTGATTCAGCCAGTCGGCGTAAGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGT‑GTAAGCGGTGTCAATGCCGTCATGAAAAGGC‑GGA‑TT‑GGAAATGATCAGATCGAATTTGCCGTCAAT‑ATGGGAAAACACGTCAC‑TGGCAACCACCGTGCCTTGCAACCGATTTTCCTGTAAAGTGCGCTTTGCGGAAGcc < 1:153488/251‑1 (MQ=255) aTGTTGATTCAGCCAGTCGGCGTAAGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGT‑GTAAGCGGTGTCAATGCCGTCATGAAAAGGC‑GGA‑TT‑GGAAATGATCAGATCGAATTTGCCGTCAAT‑ATGGGAAAACACGTCAC‑TGGCAaccac < 1:324452/204‑1 (MQ=255) tGATTCAGCCAGTCGGCGTAAGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGT‑GTAAGCGGTGTCAATGCCGTCATGAAAAGGC‑GGA‑TT‑GGAAATGATCAGATCGAATTTGCCGTCAAT‑ATGGGAAAACACGTCAC‑TGGCAACCACCGTGCCTTGCAACCGATTTTCCTGTAAAGTGCGCTTTGCGGAAGCCAACGc < 1:643489/251‑1 (MQ=255) aTTCAGCCAGTCGGCGTAAGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGT‑GTAAGCGGTGTCAATGCCGTCATGAAAAGGC‑GGA‑TT‑GGAAATGATCAGATCGAATTTGCCGTCAAT‑ATGGGAAAACACGTCAC‑TGGCAACCACCGTGCCTTGCAACCGATTTTCCTGTAAAGTGCGCTTTGCGGAAGCCAACGCCa < 1:254664/251‑1 (MQ=255) gCCAGTCGGCGTAAGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGT‑GTAAGCGGTGTCAATGCCGTCATGAAAAGGC‑GGA‑TT‑GGAAATGATCAGATCGAATTTGCCGTCAAT‑ATggg < 1:538800/171‑1 (MQ=255) ccAGTCGGCGTAAGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGT‑GTAAGCGGTGTCAATGCCGTCATGAAAAGGC‑GGA‑TT‑GGAAATGATCAGATCGAATTTGCCGTCAAT‑ATGGGAAAACACGTCAC‑TGGCAACCACCGTGCCTTGCAACCGATTTTCCTGTAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGc < 1:216724/249‑1 (MQ=255) ccAGTCGGCGTAAGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGT‑GTAAGCGGTGTCAATGCCGTCATGAAAAGGC‑GGA‑TT‑GGAAATGATCAGATCGAATTTGCCGTCAAT‑ATGGGAAAACACGTCAC‑TGGCAACCACCGTGCCTTGCAACCGATTTTCCTGTAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCg < 1:634544/250‑1 (MQ=255) tCGGCGTAAGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGT‑GTAAGCGGTGTCAATGCCGTCATGAAAAGGC‑GGA‑TT‑GGAAATGATCAGATCGAATTTGCCGTCAAT‑ATGGGAAAACACGTCAC‑TGGCAACCACCGTGCCTTGCAACCGATTTTCCTGTAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAat < 1:6991/251‑1 (MQ=255) tCGGCGTAAGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGT‑GTAAGCGGTGTCAATGCCGTCATGAAAAGGC‑GGA‑TT‑GGAAATGATCAGATCGAATTTGCCGTCAAT‑ATGGGAAAACACGTCAC‑TGGCAACCACCGTGCCTTGCAACCGATTTTCCTGTAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAat < 1:493430/251‑1 (MQ=255) gCGTAAGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGT‑GTAAGCGGGGTCAATGCCGTCATGAAAAAGC‑GGG‑TT‑GGAAATGATCAGATCGAATTTTCCGTCAAT‑ATGGGAAAAAACGTCAC‑TGGCAACCACCCTGCCTTTCAACCGATTTTCCTTTAAAGTGCGCTTTTCGGGAGCCAACGCCAAGGCGTGAatat > 1:52530/1‑250 (MQ=255) gAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGT‑GTAAACGGGGTCAATGCCGTCCTGAAAAAGC‑GGG‑TT‑TGAAAAGATAAGATCGAATTTTTCGTCCAT‑ATTGGGAAACACGGCAC‑TGGGAAACACCCAGCCTTGCAAACGATTTTTCTGTAAAAGGGGCGTTTCGGGAGCAAAAGCCAAAGCGGGGATATCCGTCATggtt > 1:287077/1‑248 (MQ=255) aaaCGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGT‑GTAAGCGGTGTCAATGCCGTCATGAAAAGGC‑GGA‑TT‑GGAAATGATCAGATCGAATTTGCCGTCAAT‑ATGGGAAAACACGTCAC‑TGGCAACCACCGTGCCTTGCAACCGATTTTCCTGTAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGAt < 1:160373/249‑1 (MQ=255) gCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGT‑GTAAGCGGGGTCAATGCCGTCCTGAAAAAGC‑GGA‑TT‑GGGAATGATCAGATCGAATTTTCCGTCAAT‑ATGGGAAAAAACGTCAC‑CGGCAACAACCCTGCCTTTCAACCGATTTTTCTGTAAAGTGCGCTTTGCGGAAGCCAACGCCAAAGCGTGGATATCCGTCATGATTAAAC‑ggggggt > 1:43963/1‑247 (MQ=255) cGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGT‑GTAAGCGGTGTCAATGCCGTCATGAAAAGGC‑GGA‑TT‑GGAAATGATCAGATCGAATTTGCCGTCAAT‑ATGGGAAAACACGTCAC‑TGGCAACCACCGTGCCTTGCAACCGATTTTCCTGTAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATC‑GGGTGTTCggg < 1:198368/251‑1 (MQ=255) cGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGT‑GTAAGCGGTGTCAATGCCGTCATGAAAAGGC‑GGA‑TT‑GGAAATGATCAGATCGAATTTGCCGTCAAT‑ATGGGAAAACACGTCAC‑TGGCAACCACCGTGCCTTGCAACCGATTTTCCTGTAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATC‑GGGTGTTCggg < 1:637680/251‑1 (MQ=255) gATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTCGCCTGTTTTATCAGTTCGTTAACGGCGGT‑GTAAGCGGGGTCAATGCCGTCATGAAAAAGG‑GGG‑TT‑GGGAAAGATCAGATCGAAATTTCCGTCAAT‑ATGGGGAAAAACGTCAC‑TGGCAACCACCGTGCCTTTCAACCGATTTTTCTGTAAAGGGCGCTTTTCGGGAGCCAACGCCAAGGCGTGAATATCCGTCATGAtt > 1:294497/1‑235 (MQ=255) aTGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGT‑GTAAGCGGTGTCAATGCCGTCATGAAAAGGC‑GGA‑TT‑GGAAATGATCAGATCGAATTTGCCGTCAAT‑ATGGGAAAACACGTCAC‑TGGCAACCACCGTGCCTTGCAACCGATTTTCCTGTAAAGTGCGCTTTGCGGAAGcc < 1:242559/204‑1 (MQ=255) aTGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGT‑GTAAGCGGTGTCAATGCCGCCATGAAAAGGC‑GGA‑TT‑GGGAAAGATCAGATCGAATTTTCCGTCAAT‑ATGGGAAAACACGTCAC‑TGGCAACCACCCTGCCTTGCAACCCATTTTTCTGTAAAGTGCGCTTTGCGGGAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATC‑GGGTGTTCggggt > 1:76843/1‑249 (MQ=255) gcAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGT‑GTAAGCGGGGTCAATGCCGGCATGAAAAAGC‑GGG‑TT‑GGAAAAGATCAGATTGAATTTGCCGTCAAA‑ATGGGAAAAAACGTCCC‑TTGCAAACACCGGGGCTTGCAACCGATTTTTCTTTAAAATGCGCTTTTCGGGAGGCAACGCCAAGGGGGGAATATTCGTCCTGATTAATT‑GGGGGGTCGGGGtgtgg > 1:588087/1‑250 (MQ=255) gCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGGTAA‑CGGTGTCAATGCCGTCATGAAAAGGC‑GGATTT‑GGAAA‑GATTAGATCGAATTTTCCGTCCAT‑AAGGGGAAAAACGTCAC‑TGGCAACCACCCTGCCTTGCCACCGATTTTTCTGTAAAATGCGCTTTTCGGGAGCCAACCCCAAAGCGGGAAAATCCGTCATGATTAATC‑GGGGGGTCGGGTtggg > 1:478732/1‑245 (MQ=255) tCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGT‑GTAAGCGGTGTCAATGCCGTCATGAAAAGGC‑GGA‑TT‑GGAAATGATCAGATCGAATTTGCCGTCAAT‑ATGGGAAAACACGTCAC‑TGGCAACCACCGTGCCTTGCAACCGATTTTCCTGTAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATC‑GGGTGTTCGGGTTGTGCTGTTg < 1:541599/251‑1 (MQ=255) cccGGTTTCAAATACCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGT‑GTAAGCGGTGTCAATGCCGTCATGAAAAGGC‑GGA‑TT‑GGAAATGATCAGATCGAATTTGCCGTCAAT‑ATGGGAAAACACGTCAC‑TGGCAACCACCGTGCCTTGCAACCGATTTTCCTGTAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATC‑GGGTGTTCGGGTTGTGCTGTTGAatat < 1:213320/250‑1 (MQ=255) gTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGT‑GTAAGCGGTGTCAATGCCGTCATGAAAAAGC‑GGA‑TT‑GGAAAAGATCAGATCGAATTTTCCGGCAAT‑ATGGGAAAAAACGTCAC‑TGGCAACCCCCGGGCCCTTCAACCGATTTTTCTGTAAAACGCGCTTTGCGGAAGCCAACGCCAAAGCGGGAAAATCCGGCATGATTAATCGGGGGGTTC‑GGTTGGGGTGGTGAATATAAcat > 1:44034/1‑249 (MQ=255) tttCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGT‑GTAAGCGGTGTCAATGCCGTCATGAAAAGGC‑GGA‑TT‑GGAAATGATCAGATCGAATTTGCCGTCAAT‑ATGGGAAAACACGTCAC‑TGGCAACCACCGTGCCTTGCAACCGATTTTCCTGTAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATC‑GGGTGTTCGGGTTGTGCTGTTGAATATAACTGc < 1:258680/251‑1 (MQ=255) ccATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGT‑GTAAGCGGTGTCAATGCCGTCATGAAAAGGC‑GGA‑TT‑GGAAATGATCAGATCGAATTTGCCGTCAAT‑ATGGGAAAACACGTCAC‑TGGCAACCACCGTGCCTTGCAACCGATTTTCCTGTAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATC‑GGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCAcc < 1:644109/250‑1 (MQ=255) ccTGTTTTATCAGTTCGTTAACGGCGGT‑GTAAGCGGTGTCAATGCCGTCATGAAAAGGC‑GGA‑TT‑GGAAATGATCAGATCGAATTTGCCGTCAAT‑ATGGGAAAACACGTCAC‑TGGCAACCACCGTGCCTTGCAACCGATTTTCCTGTAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATC‑GGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGcgccg < 1:201159/251‑1 (MQ=255) gTTTTATCAGTTCGTTAACGGCGGT‑GTAAGCGGGGTCAATGGCGTCATGAAAAAGC‑GGG‑TT‑GGAAAAGATTAAATCGAATTTGTCGGCAAT‑ATGGGGAAAAACGGCAA‑TGGCAAACCCCCTGCCCTGCAACCGATTTTTCTGTAAAATGCGCTTTTCGGGAGGCAAAGCCAAAGCGGGAATATCCGTCATGAGTAATC‑GGGGGTTCGGGGTGTGCTGTTGGATATAAATGCCAATAAACCCTGcgccgcc > 1:380096/1‑249 (MQ=255) gTTTTATCAGTTCGTTAACGGCGGT‑GTAAGCGGGGTCAAAGCCGTCATGAAAAAGC‑GGA‑TT‑GGGAAAGATCAGATCGAATTTGTCGTCAAT‑ATTGGGAAACACGGCCCTTGGC‑ACCCCCGCGCCTTGCCACCGATTTTCCTTTAAAATGCGCTTTTCGGGAGCCCACGCCAAGGCGTGAAAATCCGTCATGATTAATC‑GGGGGTTCCGGGTGTGCTGTTGTATATAACTGCCAATCACCCCCGcgccgccg > 1:452989/1‑249 (MQ=255) gTTTTATCAGTTCGTTAACGGCGGT‑GTAAGAGGGGTCCATGCCGTCATGGAAAGGC‑GGG‑TT‑GGGAATGATCAGATCGAATTTGCCGGCAAA‑ATGGGGAAACACGTCCC‑TGGGAAACACCGTGCCCTGCAACCGATTTTTCTTTAAAATGCGCTTTGCGGGAGCCAAAGCCCAGGCGGGGATATACGTCATGATTAATT‑GGGTGGTCGGGGTGTGGTGGTGAATATAACTGCCAATCAccccccgtctcttt > 1:278607/1‑241 (MQ=255) ttAACGGCGGT‑GTAAGCGGTGTCAATGCCGTCATGAAAAGGC‑GGA‑TT‑GGAAATGATCAGATCGAATTTGCCGTCAAT‑ATGGGAAAACACGTCAC‑TGGCAACCACCGTGCCTTGCAACCGATTTTCCTGTAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATC‑GGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAacac < 1:598440/251‑1 (MQ=255) ttAACGGCGGT‑GTAAGCGGTGTCAATGCCGTCATGAAAAGGC‑GGA‑TT‑GGAAATGATCAGATCGAATTTGCCGTCAAT‑ATGGGAAAACACGTCAC‑TGGCAACCACCGTGCCTTGCAACCGATTTTCCTGTAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATC‑GGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAacac < 1:422533/251‑1 (MQ=255) tAACGGCGGT‑GTAAGCGGTGTCAATGCCGTCATGAAAAGGC‑AGA‑TTGGGAAATGATCAGATCGAATTTGCCGTCAAT‑ATGGGAAAACACGTCAC‑TGGCAACCACCGTGCCTTGCAACCGATTTTCCTGTAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATC‑GGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAacac < 1:420280/251‑1 (MQ=255) gt‑gtAAGCGGTGTCAATGCCGTCATGAAAAGGC‑GGA‑TT‑GGAAATGATCAGATCGAATTTGCCGTCAAT‑ATGGGAAAACACGTCAC‑TGGCAACCACCGTGCCTTGCAACCGATTTTCCTGTAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATC‑GGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCcg > 1:488631/1‑250 (MQ=255) | TTAAATAATAAGTAGAATTCTAACTAAAAGGCTTCGCTAAGCCAGCGGAATTTCGTGCACACTTAACTCATCTGCGCACCGAATATACCTTAAACTTGTTATTTCTCGCCAGGACGTCATGACTGCCGAAATGTTGATTCAGCCAGTCGGCGTAAGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGT‑GTAAGCGGTGTCAATGCCGTCATGAAAAGGC‑GGA‑TT‑GGAAATGATCAGATCGAATTTGCCGTCAAT‑ATGGGAAAACACGTCAC‑TGGCAACCACCGTGCCTTGCAACCGATTTTCCTGTAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATC‑GGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCG > 1/1984852‑1985340 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |