| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | 1 | 1,910,121 | 0 | T | G | 44.6% | 124.3 / 11.8 | 65 | D381A (GAC→GCC) | unknown | Replication‑associated recombination protein RarA |
| Reads supporting (aligned to +/- strand): ref base T (14/22); new base G (29/0); total (43/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.25e-08 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GTGCAAGCGCAGCGCAGTAAAAGTAGGTCGATGCCGAAACAAACCTATTTATATCGCTTCTTACCACTTTCCTGATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGC‑GGGAA‑GTAGTTTTCGCCGGCAGCGTA‑GGC‑GTTAGGTTCATTGTGGGCGTAGCGGTATTCGGCGCCGAAGCCGAGGTCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGACGTCGAAATCGGC‑GGATTC‑GGTT‑G‑CCAATTTTTTG‑GCGGCTTTGAAAGCGAGATAAACGGCGTTGCTTTTCGGTGCGACGGCTAAATACACAATGGCTTGCGCAATAGCGCGTTCGCCTTCGGCAGCGCCGACGCGGGTGAAACAATCCCAGGCGGCAAGTGCCACCTGCATGGCGCGCGGGTCAGCG > 1/1909878‑1910351 | gTGCAAGCGCAGCGCAGTAAAAGTAGGTCGATGCCGAAACAAACCTATTTATATCGCTTCTTACCACTTTCCTGATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGC‑GGGAA‑GCAGTTTTTGCCGGCCGCGAC‑GGC‑GGTAGGTTCCTTGTGGGGGGAGCGGGATTTGGCGCCGAAGCCGAGGGCTTTCAt > 1:565392/1‑251 (MQ=255) gcgcagcgcaGTAAAAGTAGGTCGATGCCGAAACAAACCTATTTATATCGCTTCTTACCACTTTCCTGATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGC‑GGGAA‑GTAGTTTTTGCCGGCGGCGGA‑GGC‑GTTTGGGTTTTTGTGGGGGGTGGGGGATTCGGCGCCGGAGCCGCGGGCTTTTATGacg > 1:24725/1‑247 (MQ=255) cagTAAAAGTAGGTCGATGCCGAAACAAACCTATTTATATCGCTTCTTACCACTTTCCTGATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGC‑GGGGA‑GTAGTTTTTGCCGGGAGCGTA‑GGG‑GGTAGGGTCTTTGTGGGGGTTGCGGGATTTGGCGCCGGAGCCGGGGGCTTTCATGAAGCTGGTCgggg > 1:630821/1‑251 (MQ=255) cagTAAAAGTAGGTCGATGCCGAAACAAACCTATTTATATCGCTTCTTACCACTTTCCTGATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGC‑GGGAA‑GGAATTTTTGCCGGCAGCGTT‑GGCGGTTGGGTTC‑TTGTGGGGGGAGCGGGGTTTGGCGCCGAAGGCCAGGGCTTTTAAGTAGGTGGTCgggg > 1:5927/1‑251 (MQ=255) aaGTAGGTCGATGCCGAAACAAACCTATTTATATCGCTTCTTACCACTTTCCTGATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGC‑GGGAA‑GTAGGTTTCGCCGGCCGCGGA‑GGG‑GTTAGGGTCCTTGTGGGGGTAGCGGGATTTGGGGCCGCAGCCGAGGGCTTTCATGAAGCGGGGCGGGGCGTTgg > 1:183583/1‑250 (MQ=255) ggTCGATGCCGAAACAAACCTATTTATATCGCTTCTTACCACTTTCCTGATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGC‑GGGAA‑GGAGGTTTTGCCGGCAGCGGA‑GGG‑GTTAGGGTCATTGTGGGGGGAGCGGGATTTGGCGCCGAAGCCGAGGGCTTTTATTAAGCTGGGCGGGGCGGTGCggggg > 1:279301/1‑248 (MQ=255) aaacaaacCTATTTATATCGCTTCTTACCACTTTCCTGATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGC‑GGGAA‑GTAGTTTTCGCCGGCAGCGTA‑GGC‑GTTAGGTTCATTGTGGGCGTAGCGGTATTCGGCGCCGAAGCCGAGGTCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGa < 1:71662/250‑1 (MQ=255) aacCTATTTATATCGCTTCTTACCACTTTCCTGATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGC‑GGGAA‑GTAGTTTTCGCCGGCAGCGTA‑GGC‑GTTAGGTTCATTGTGGGCGTAGCGGTATTCGGCGCCGAAGCCGAGGTCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAggg < 1:626917/244‑1 (MQ=255) aTTTATATCGCTTCTTACCACTTTCCTGATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGC‑GGGAA‑GTAGGTTTTGCCGGCAGCGTAGGGG‑GTTGGGTT‑ATTGTGGGGGTAGCGGGATTCGGCGCCGAAGCCGAGGGCTTTCTTGAAGCTGGTCGGGGCGTTGCGGGGATGTTCAGGGGCGTCGGAATcg > 1:503962/1‑251 (MQ=255) gCTTCTTACCACTTTCCTGATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGC‑GGGAA‑GTAGTTTTCGCCGGCAGCGGA‑GGC‑GTTAGGTTCATTGTGGGGGTAGCGGGATTTGGCGCCGAAGCCGAGGGCTTTTATGAAGCTGGTCGGGGCGTTGCGGGGATGTTCAGGGACGTCGAAATCGGC‑GGGTTC‑g > 1:471977/1‑251 (MQ=255) tcttACCACTTTCCTGATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGC‑GGGAA‑GTAGTTTTTGCCGGCAGCGTA‑GGC‑GTTTGGGTTATTTTGGGGGGAGGGGGATTTGGCGCCGGAGCCGAGGGCTTTCCTGAAGCTGGGCGGGGGGGTGCGGGGATGTTTAGGGGCGGCGAAATcggc‑ggggttgggg > 1:463377/1‑243 (MQ=255) tcttACCACTTTCCTGATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGC‑GGGAA‑GTAGGTTTTGCCGGCAGCGGA‑GGC‑GGTAGGGTCATTGTGGGCGTAGCGGGATTCGGCGCCGAAGCCGAGGGCTTTCATGAAGCTGGGCGGGGCGGTGCGGAGAGGTTCAGGGACGTCGAAATcggc‑gggtttgggt > 1:312269/1‑243 (MQ=255) tattaacACTTTCCTGATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGC‑GGGAA‑GTAGTTTTTGCCGGCAGCGTA‑GGG‑GTTTGGGTTATTGTGGGGGTGGCGGGATTTGGGGCCGCAGCCGAGGGCTTTCATGAAGCTGGTCGGGGGGTTGCGGGGATGTTCAGGGGCGTCGAAATCGGC‑GGGTTT‑GGtt > 1:42473/7‑251 (MQ=255) ttCCTGATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGGGAA‑G‑AGGTTTCGCCGGGAGGGTA‑GGC‑GTTTGGTTCATTTTGGGCGGCGCGGGTTTTGGGGCCGAAACCGGGGGCTTTTCTGAAGCTGGGCGGGGGGGTGGGGGGATGTTCTGGGGCGTCGAAATcggc‑ggggttcgttggcgattttt > 1:624596/1‑233 (MQ=255) ttCCTGATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGC‑GGGGA‑GGAGGTTTTGCCGGGCGCGGA‑GGC‑GTTTGGGTCATTGTTGGGGGAGGGGGATTTGGGGCCGGAGCCGAGGGCTTTCATGAAGCTGGGCGGGGGGGTGCGGGGGTGTTTAGGGGCGTCGAAATcggc‑ggggttgggtttgtcttttt > 1:36701/1‑233 (MQ=255) ttCCTGATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGC‑GGGAA‑GTAGTTTTTGCCGGCCGCGGA‑GGC‑GGTTGGGTCATTGTGGGGGTAGCGGGATTCGGCGCCGAAGCCGAGGGCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGGCGGCGAAATCGGC‑GGGTTCGGGTT‑G‑‑CAAttttt > 1:428845/1‑251 (MQ=255) ttCCTGATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGC‑GGGAA‑GGAGTTTTCGCCGGCAGCGTA‑GGC‑GTTTGGGTCATTGTGGGCGGAGCGGGATTCGGCGCCGAAGCCGCGGGCTTTCATGAAGCTGGGCGGGGCGGTGCGGGGAGGTTCAGGGGCGGCGAAATCGGC‑GGGTTT‑GGTT‑G‑GCCAttttt > 1:430749/1‑251 (MQ=255) gATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGC‑GGGGA‑GGAGTTTTCGCCGGCCGCGGA‑GGC‑GGTTGGTTCATTGTGGGCGGAGGGGGATTTGGCGCCGAAGCCGAGGGCTTTTATGAAGCCGGGCGGGGGGGTGCGGGGGTGGTCAGGGGCGTCGAAAACCGCGGGGTTC‑GGTG‑G‑CCATTTTTTTg‑gg > 1:415920/1‑250 (MQ=255) gATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGC‑GGGAA‑GTAGTTTTCGCCCGCAGCGGA‑GGG‑GGTAGGTTCATTTTGGGCGGAGCGGTATTTGGCGCCGAAGCCGAGGGCTTTTATGAAGCTGGTCGGGGCGTTGCGGGGATGTTCCGGGGCGTCGAAAAcggc‑ggggttgggtggccatttttttgcg > 1:557344/1‑228 (MQ=255) gATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGC‑GGGAA‑GAAGGTTTTTCCCGCAGCGGA‑GGC‑GTTTGGTTCCTTGTGGGCGGAGCGGGTTTCGGCGCCCAAGCCGAGGGCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGAAGTTCAGGGGCGTCGAAATCGGC‑GGGTTC‑ggggggccatttttttgcg > 1:473487/1‑234 (MQ=255) aTCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGC‑GGGAAGGTGGTTTT‑GCCGGCCACGCG‑GGC‑GTTTGGTTTATTGTGGGCGGAGCGGTATTCCGCGCCGAAGCCGAGGGCTTTCATGAAGCGGGTCGGGGGGTTGCGGGGATGTTCCAGGACGTCGAAATTGGC‑GGGGTC‑GGGT‑G‑GCAAATTTTTt‑gcgg > 1:438556/1‑251 (MQ=255) aaCCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGC‑GGGGA‑GTAGTTTTTGCCGGCAGCGTA‑GGC‑GGTAGGGTCATTGTGGGCGTAGCGGGATTTGGCGCCGAAGCCGAGGGCTTTCATTAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGACGTCGAAATCGGC‑GGGGTC‑GGGT‑G‑CCAATTTTTTT‑GGGGCttt > 1:125074/1‑251 (MQ=255) ccGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGC‑GGGAA‑GTAGTTTTCGCCGGCAGCGTA‑GGC‑GTTAGGTTCATTGTGGGCGTAGCGGTATTCGGCGCCGAAGCCGAGGTCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGACGTCGAAATCGGC‑GGATTC‑GGTT‑G‑CCAATTTTTTG‑GCGGCTTTGAAAg < 1:278616/251‑1 (MQ=255) ccGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGC‑GGGAA‑GTAGTTTTCGCCGGCAGCGTA‑GGC‑GTTAGGTTCATTGTGGGCGTAGCGGTATTCGGCGCCGAAGCCGAGGTCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGACGTCGAAATCGGC‑GGATTC‑GGTT‑G‑CCAATTTTTTG‑GCGGCTTTGAAAg < 1:60984/251‑1 (MQ=255) cATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGC‑GGGAA‑GGAGTTTTCGCCGGCAGCGTA‑GGC‑GGTAGGTTCATTGTGGGGGGAGCGGGATTCGGCGCCGAAGCCGAGGGCTTTCATGAAGCTGGGCGGGGCGGTGCGGGGGTGTTCAGGGACGGCGAAAt > 1:13260/1‑205 (MQ=255) cATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGC‑GGGAA‑GGAGGTTTTGCCGGCAGCGGA‑GGG‑GGTAGGTTTCTTGTGGGCGTAGCGGGATTTGGCGCCGGAGCCGAGGGCTTTTATGAAGCTGGGCGGGGCGTTGCGGGGGTGTTTAGGGGCGGCGGAATCGGC‑GGGTTT‑GGGT‑GCCCAATTTTTTgcggcgttttaagggagaga > 1:641156/1‑232 (MQ=255) tCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGC‑GGGAA‑GTAGTTTTCGCCGGCAGCGTA‑GGC‑GTTAGGTTCATTGTGGGCGTAGCGGTATTCGGCGCCGAAGCCGAGGTCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGACGTCGAAATCGGC‑GGATTC‑GGTT‑G‑CCAATTTTTTG‑GCGGCTTTGAAAGCGAGATAAAc < 1:502651/251‑1 (MQ=255) cAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGC‑GGGAA‑GTAGTTTTCGCCGGCAGCGTA‑GGC‑GTTAGGTTCATTGTGGGCGTAGCGGTATTCGGCGCCGAAGCCGAGGTCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGACGTCGAAATCGGC‑GGATTC‑GGTT‑G‑CCAATTTTTTG‑GCGGCTTTGAAAGCGAGATAAACgg < 1:17638/251‑1 (MQ=255) aaCTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGC‑GGGAA‑GTAGTTTTTGCCGGCAGCGTA‑GGG‑GGTAGGGTCATTTTGGGCGGAGCGGGATTCGGCGCCGCAGACGAGGGCTTTCATGAAGCTGGTCGGGGCGGTGCGGAGATGTTCAGGGACGTCGAAATCGGC‑GGGATC‑GGGT‑G‑CCCCTTTTTTT‑GGGGCtttttaaaggggataaaagg > 1:83069/1‑233 (MQ=255) ccTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGC‑GGGGA‑GTAGTTTTTGCCGGCAGCGTA‑GGC‑GTTTGGTTCATTGTGGGCGGAGCGGGATTCGGCGCCGAAGCCGAGGGCTTTCATGAAGCTGGTCGGGGGGGTGCGGAGATGTTTAGGGACGGCGGAATCGGG‑GGGTTCGGGTG‑G‑CCATTTTTTTG‑GGGGGTTTG‑AAGGGAGATAAAAGGCGGTGCtt > 1:368799/1‑251 (MQ=255) cttaatTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGC‑GGGAA‑GGAGGTTTTGCCGGCAGCGGA‑GGG‑GTTAGGGTCATTGTGGGGGGAGCGGGATTCGGGGCCGAAGCCGGGGGCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGGCGTCGAAATAGGC‑GGGTTT‑GGGT‑G‑GCAAATTTTTGTGGGGGTTTGAAA‑AGAGATAAAAGGCGGTGCTTTTTGGTg > 1:628433/1‑251 (MQ=255) ttCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGC‑GGGAA‑GTAGTTTTCGCCGGCAGCGTA‑GGC‑GGTAGGTTCATTGTGGGGGTAGCGGTATTCGGCGCCGAAGCCGAGGGCTTTTATGAAGCTGGTCGGGGCGTTGCGGAGATGGTCAGGGGCGTCGAAATCGGC‑GGATTT‑GGGT‑G‑CCCATTTTTTT‑GCGGGCTTGAAAGCGGGATAAAAGGCGGTGCTTTTTGGTGcggcgg > 1:196515/1‑251 (MQ=255) ctctGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGC‑GGGGA‑GGAGTTTTTGCCGGCCGCGGA‑GGC‑GGTTGGGTCATTGTGGGGGTAGCGGGATTCGGGGCCGGAGCCGGGGGCTTTTATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGACGTCGGAAt > 1:405903/1‑171 (MQ=255) ctctGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGC‑GGGAA‑GTAGTTTTCGCCGGCAGCGTA‑GGC‑GTTAGGTTCATTGTGGGCGTAGCGGTATTCGGCGCCGAAGCCGAGGTCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGACGTCGAAATCGGC‑GGATTC‑GGTT‑G‑CCAATTTTTTG‑GCGGCTTTGAAAGCGAGATAAACGGCGTTGCTTTTCGGTGCGACGGCTAAATac < 1:230797/251‑1 (MQ=255) ctctGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGC‑GGGAA‑GTAGTTTTCGCCGGCAGCGTA‑GGC‑GTTAGGTTCATTGTGGGCGTAGCGGTATTCGGCGCCGAAGCCGAGGTCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGACGTCGAAATCGGC‑GGATTC‑GGTT‑G‑CCAATTTTTTG‑GCGGCTTTGAAAGCGAGATAAACGGCGTTGCTTTTCGGTGCGACGGCTAAATac < 1:255827/251‑1 (MQ=255) ctGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGC‑GGGGA‑GTAGGTTTTGCCGGCAGCGTA‑GGG‑GTTAGGTTCATTGTGGGGGGAGCGGGATTTGGCGCCGAAGCCGAGGGCTTTCATGAAGCTGGTCGGGGGGTTGCGGGGGTGTTCAGGGGCGTCGGAAACGGC‑GGGTTC‑GGTTGG‑CCATTTTTTTG‑G‑GGCTTTTTAAAAGGGATAAAAGGCGGTGCTTTTTTGTGGGGCGGCTAAATaaaa > 1:510407/1‑248 (MQ=255) ctGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGC‑GGGAA‑GTAGTTTTCGCCGGCAGCGTA‑GGC‑GTTAGGTTCATTGTGGGCGTAGCGGTATTCGGCGCCGAAGCCGAGGTCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGACGTCGAAATCGGC‑GGATTC‑GGTT‑G‑CCAATTTTTTG‑GCGGCTTTGAAAGCGAGATAAACGGCGTTGCTTTTCGGTGCGACGGCTAAATacac < 1:822/251‑1 (MQ=255) ctGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGC‑GGGAA‑GTAGTTTTCGCCGGCAGCGTA‑GGC‑GTTAGGTTCATTGTGGGCGTAGCGGTATTCGGCGCCGAAGCCGAGGTCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGACGTCGAAATCGGC‑GGATTC‑GGTT‑G‑CCAATTTTTTG‑GCGGCTTTGAAAGCGAGATAAACGGCGTTGCTTTTCGGTGCGACGGCTAAATacac < 1:254164/251‑1 (MQ=255) tggtggTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGC‑GGGAA‑GTAGTTTTCGCCGGCAGCGTA‑GGC‑GTTAGGTTCATTGTGGGCGTAGCGGTATTCGGCGCCGAAGCCGAGGTCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGACGTCGAAATCGGC‑GGATTC‑GGTT‑G‑CCAATTTTTTG‑GCGGCTTTGAAAGCGAGATAAACGGCGTTGCTTTTCGGTGCGACGGCTAAATAcaca < 1:210631/251‑1 (MQ=255) tggtCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGC‑GGGAA‑GTAGTTTTCGCCGGCAGCGTA‑GGC‑GTTAGGTTCATTGTGGGCGTAGCGGTATTCGGCGCCGAAGCCGAGGTCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGACGTCGAAATCGGC‑GGATTC‑GGTT‑G‑CCAATTTTTTG‑GCGGCTTTGAAAGCGAGATAAACGGCGTTGCTTTTCGGTGCGACGGCTAAATACACAAt < 1:530893/250‑1 (MQ=255) tggtCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGC‑GGGAA‑GTAGTTTTCGCCGGCAGCGTA‑GGC‑GTTAGGTTCATTGTGGGCGTAGCGGTATTCGGCGCCGAAGCCGAGGTCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGACGTCGAAATCGGC‑GGATTC‑GGTT‑G‑CCAATTTTTTG‑GCGGCTTTGAAAGCGAGATAAACGGCGTTGCTTTTCGGTGCGACGGCTAAATACACAAt < 1:308066/250‑1 (MQ=255) tCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGC‑GGGAA‑GTAGTTTTCGCCGGCAGCGTA‑GGC‑GTTAGGTTCATTGTGGGCGTAGCGGTATTCGGCGCCGAAGCCGAGGTCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGACGTCGAAATCGGC‑GGATTC‑GGTT‑G‑CCAATTTTTTG‑GCGGCTTTGAAAGCGAGATAAACGGCGTTGC‑TTTCGGTGCGACGGCTAAATACACAATGGCt < 1:621160/250‑1 (MQ=255) aaaTAGAATTGGGTGTCTTTCAGTTGTGGC‑GGGAA‑GGAGTTTTTGCCCGCCGCGGA‑GGC‑GGTTGGGTCCTTGTGGGCGGAGCGGGATTCGGGGCCGGAGCCGAGGGCTTTCATGAAGCTGGTCGGGGGGTTGCGGGGGTGTTCCGGGGCGTCGGAAACGGC‑GGGGTCGGGTT‑C‑CCAATTTTTTG‑GGGGCTTTTAAA‑CGAGGAAAAAAGCGGTGCTTTTTGGTGCGGCGGGCCAATACACaaaaggctgtg > 1:392409/1‑241 (MQ=255) aTAGAATTGGGTGTCTTTCAGTTGTGGC‑GGGAA‑GTAGTTTTCGCCGGCAGCGTA‑GGC‑GTTAGGTTCATTGTGGGCGTAGCGGTATTCGGCGCCGAAGCCGAGGTCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGACGTCGAAATCGGC‑GGATTC‑GGTT‑G‑CCAATTTTTTG‑GCGGCTTTGAAAGCGAGATAAACGGCGTTGCTTTTc < 1:119623/217‑1 (MQ=255) aGAATTGGGTGTCTTTCAGTTGTGGC‑GGGAA‑GTAGTTTTCGCCGGCAGCGTA‑GGC‑GTTAGGTTCATTGTGGGCGTAGCGGTATTCGGCGCCGAAGCCGAGGTCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGACGTCGAAATCGGC‑GGATTC‑GGTT‑G‑CCAATTTTTTG‑GCGGCTTTGAAAGCGAGATAAACGGCGTTGCTTTTCGGTGCGACGGCTAAATACACAATgg < 1:56234/240‑1 (MQ=255) aaTTGGGTGTCTTTCAGTTGTGGC‑GGGAA‑GTAGTTTTCGCCGGCAGCGTA‑GGC‑GTTAGGTTCATTGTGGGCGTAGCGGTATTCGGCGCCGAAGCCGAGGTCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGACGTCGAAATCGGC‑GGATTC‑GGTT‑G‑CCAATTTTTTG‑GCGGCTTTGAAAGCGAGATAAACGGCGTTGCTTTTCGGTGCGACGGCTAAATACACAATGGCTTGCGCAATAg < 1:223907/250‑1 (MQ=255) aaTTGGGTGTCTTTCAGTTGTGGC‑GGGAA‑GTAGTTTTCGCCGGCAGCGTA‑GGC‑GTTAGGTTCATTGTGGGCGTAGCGGTATTCGGCGCCGAAGCCGAGGTCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGACGTCGAAATCGGC‑GGATTC‑GGTT‑G‑CCAATTTTTTG‑GCGGCTTTGAAAGCGAGATAAACGGCGTTGCTTTTCGGTGCGACGGCTAAATACACAATGGCTTGCGCAATAg < 1:409333/250‑1 (MQ=255) aaTTGGGTGTCTTTCAGTTGTGGC‑GGGAA‑GTAGTTTTCGCCGGCAGCGTA‑GGC‑GTTAGGTTCATTGTGGGCGTAGCGGTATTCGGCGCCGAAGCCGAGGTCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGACGTCGAAATCGGC‑GGATTC‑GGTT‑G‑CCAATTTTTTG‑GCGGCTTTGAAAGCGAGATAAACGGCGTTGCTTTTCGGTGCGACGGCTAAATACACAATGGCTTGCGCAATAg < 1:462041/250‑1 (MQ=255) aTTGGGTGTCTTTCAGTTGTGGC‑GGGAA‑GTAGTTTTCGCCGGCAGCGTA‑GGC‑GTTAGGTTCATTGTGGGCGTAGCGGTATTCGGCGCCGAAGCCGAGGTCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGACGTCGAAATCGGC‑GGATTC‑GGTT‑G‑CCAATTTTTTG‑GCGGCTTTGAAAGCGAGATAAACGGCGTTGCTTTTCGGTGCGACGGCTAAATACACAAt < 1:523959/235‑1 (MQ=255) gAA‑GTAGTTTTCGCCGGCAGCGTA‑GGC‑GTTAGGTTCATTGTGGGCGTAGCGGTATTCGGCGCCGAAGCCGAGGTCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGACGTCGAAATCGGC‑GGATTC‑GGTT‑G‑CCAATTTTTTG‑GCGGCTTTGAAAGCGAGATAAACGGCGTTGCTTTTCGGTGCGACGGCTAAATACACAATGGCTTGCGCAATAGCGCGTTCGCCTTCGGCAGCGCCGAcg > 1:209445/1‑250 (MQ=255) gtagtTTTCGCCGGCAGCGTA‑GGC‑GTTAGGTTCATTGTGGGCGTAGCGGTATTCGGCGCCGAAGCCGAGGTCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGACGTCGAAATCGGC‑GGATTC‑GGTT‑G‑CCAATTTTTTG‑GCGGCTTTGAAAGCGAGATAAACGGCGTTGCTTTTCGGTGCGACGGCTAAATACACAATGGCTTGCGCAATAGCGCGTTCGCCTTCGGCAGCGCCGACGCggg > 1:596548/1‑251 (MQ=255) agtTTTCGCCGGCAGCGTA‑GGC‑GTTAGGTTCATTGTGGGCGTAGCGGTATTCGGCGCCGAAGCCGAGGTCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGACGTCGAAATCGGC‑GGATTC‑GGTT‑G‑CCAATTTTTTG‑GCGGCTTTGAAAGCGAGATAAACGGCGTTGCTTTTCGGTGCGACGGCTAAATACACAATGGCTTGCGCAATAg > 1:145740/1‑219 (MQ=255) cAGCGTA‑GGC‑GTTAGGTTCATTGTGGGCGTAGCGGTATTCGGCGCCGAAGCCGAGGTCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGACGTCGAAATCGGC‑GGATTC‑GGTT‑G‑CCAATTTTTTG‑GCGGCTTTGAAAGCGAGATAAACGGCGTTGCTTTTCGGTGCGACGGCTAAATACACAATGGCTTGCGCAATAGCGCGTTCGCCTTCGGCAGCGCCGACGCGGGTGAAACAATCCCAg > 1:388473/1‑251 (MQ=255) gCGTA‑GGC‑GTTAGGTTCATTGTGGGCGTAGCGGTATTCGGCGCCGAAGCCGAGGTCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGACGTCGAAATCGGC‑GGATTC‑GGTT‑G‑CCAATTTTTTG‑GCGGCTTTGAAAGCGAGATAAACGGCGTTGCTTTTCGGTGCGACGGCTAAATacac < 1:176054/188‑1 (MQ=255) gCGTA‑GGC‑GTTAGGTTCATTGTGGGCGTAGCGGTATTCGGCGCCGAAGCCGAGGTCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGACGTCGAAATCGGC‑GGATTC‑GGTT‑G‑CCAATTTTTTG‑GCGGCTTTGAAAGCGAGATAAACGGCGTTGCTTTTCGGTGCGACGGCTAAATACACAATGGCTTGCGCAATAGCGCGTTCGCCTTCGGCAGCGCCGACGCGGGTGAAACAATCCCAgg > 1:600501/1‑250 (MQ=255) gCGTA‑GGC‑GTTAGGTTCATTGTGGGCGTAGCGGTATTCGGCGCCGAAGCCGAGGTCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGACGTCGAAATCGGC‑GGATTC‑GGTT‑G‑CCAATTTTTTG‑GCGGCTTTGAAAGCGAGATAAACGGCGTTGCTTTTCGGTGCGACGGCTAAATACACAATGGCTTGCGCAATAGCGCGTTCGCCTTCGGCAGCGCCGACGCGGGTGAAACAATCCCAgg > 1:354110/1‑250 (MQ=255) gCGTA‑GGC‑GTTAGGTTCATTGTGGGCGTAGCGGTATTCGGCGCCGAAGCCGAGGTCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGACGTCGAAATCGGC‑GGATTC‑GGTT‑G‑CCAATTTTTTG‑GCGGCTTTGAAAGCGAGATAAACGGCGTTGCTTTTCGGTGCGACGGCTAAATACACAATGGCTTGCGCAATAGCGCGTTCGCCTTCGGCAGCGCCGACGCGGGGGAAACAATCCCAgg > 1:583250/1‑250 (MQ=255) ggC‑GTTAGGTTCATTGTGGGCGTAGCGGTATTCGGCGCCGAAGCCGAGGTCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGACGTCGAAATCGGC‑GGATTC‑GGTT‑G‑CCAATTTTTTG‑GCGGCTTTGAAAGCGAGATAAACGGCGTTGCTTTTCGGTGCGACGGCTAAATACACAATGGCTTGCGCAATAGCGCGTTCGCCTTCGGCAGCGCCGACGCGGGTGAAACAATCCCAGGCGGCaa > 1:317617/1‑251 (MQ=255) ggTTCATTGTGGGCGTAGCGGTATTCGGCGCCGAAGCCGAGGTCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGACGTCGAAATCGGC‑GGATTC‑GGTT‑G‑CCAATTTTTTG‑GCGGCTTTGAAAGCGAGATAAACGGCGTTGCTTTTCGGTGCGACGGCTAAATACACAATGGCTTGCGCAATAGCGCGTTCGCCTTCGGCAGCGCCGACGCGGGTGAAACAATCCCAGGCGGCAAGTGCCa > 1:330159/1‑250 (MQ=255) gTTCATTGTGGGCGTAGCGGTATTCGGCGCCGAAGCCGAGGTCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGACGTCGAAATCGGC‑GGATTC‑GGTT‑G‑CCAATTTTTTG‑GCGGCTTTGAAAGCGAGATAAACGGCGTTGCTTTTCGGTGCGACGGCTAAATACACAATGGCTTGCGCAATAGCGCGTTCGCCTTCGGCAGCGCCGAcgc > 1:160454/1‑219 (MQ=255) gTTCATTGTGGGCGTAGCGGTATTCGGCGCCGAAGCCGAGGTCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGACGTCGAAATCGGC‑GGATTC‑GGTT‑G‑CCAATTTTTTG‑GCGGCTTTGAAAGCGAGATAAACGGCGTTGCTTTTCGGTGCGACGGCTAAATACACAATGGCTTGCGCAATAGCGCGTTCGCCTTCGGCAGCGCCGACGCGGGTGAAACAATCCCAGGCGGCAAGTGCCAc > 1:197919/1‑250 (MQ=255) ttGTGGGCGTAGCGGTATTCGGCGCCGAAGCCGAGGTCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGACGTCGAAATCGGC‑GGATTC‑GGTT‑G‑CCAATTTTTTG‑GCGGCTTTGAAAGCGAGATAAACGGCGTTGCTTTTCGGTGCGACGGCTAAATACACAATGGCTTGCGCAATAGCGCGTTCGCCTTCGGCAGCGCCGACGCGGGTGAAACAATCCCAGGCGGCAAGTGCCACCTGCAt < 1:214842/251‑1 (MQ=255) aTTCGGCGCCGAAGCCGAGGTCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGACGTCGAAATCGGC‑GGATTC‑GGTT‑G‑CCAATTTTTTG‑GCGGCTTTGAAAGCGAGATAAACGGCGTTGCTTTTCGGTGCGACGGCTAAATACACAATGGCTTGCGCAATAGCGCGTTCGCCTTCGGCAGCGCCGACGCGGGTGGAACAATCCCAGGCGGCAAGTGCCACCTGCATGGCGCGCGGGTCAGCg > 1:287510/1‑251 (MQ=255) ccGAAGCCGAGGTCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGACGTCGAAATCGGC‑GGATTC‑GGTT‑G‑CCAATTTTTTG‑GCGGCTTTGAAAGCGAGATAAACGGCGTTGCTTTTCGGTGCGACGGCTAAATACACAATGGCTTGCGCAATAGCGCGTTCGCCTTCGGCAGCGCCGACGCGGGTGAAACa > 1:175331/1‑200 (MQ=255) gTCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGACGTCGAAATCGGC‑GGATTC‑GGTT‑G‑CCAATTTTTTG‑GCGGCTTTGAAAGCGAGATAAACGGCGTTGCTTTTCGGTGCGACGGCTAAATACACAATGGCTTGCGCAATAGCGCGTTCGCCTTCGGCAGCGCCGACGCGGGGGAAAc > 1:400244/1‑188 (MQ=255) | GTGCAAGCGCAGCGCAGTAAAAGTAGGTCGATGCCGAAACAAACCTATTTATATCGCTTCTTACCACTTTCCTGATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGC‑GGGAA‑GTAGTTTTCGCCGGCAGCGTA‑GGC‑GTTAGGTTCATTGTGGGCGTAGCGGTATTCGGCGCCGAAGCCGAGGTCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGACGTCGAAATCGGC‑GGATTC‑GGTT‑G‑CCAATTTTTTG‑GCGGCTTTGAAAGCGAGATAAACGGCGTTGCTTTTCGGTGCGACGGCTAAATACACAATGGCTTGCGCAATAGCGCGTTCGCCTTCGGCAGCGCCGACGCGGGTGAAACAATCCCAGGCGGCAAGTGCCACCTGCATGGCGCGCGGGTCAGCG > 1/1909878‑1910351 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |