Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,910,059	0	C	T	41.5%	61.2 / 22.7	54	E402K (GAA→AAA)	unknown	Replication‑associated recombination protein RarA
Reads supporting (aligned to +/- strand):  ref base C (11/20);  new base T (22/0);  total (34/20)
Fisher's exact test for biased strand distribution p-value = 4.35e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GGTACGAAGTGCAAGCGCAGCGCAGTAAAAGTAGGTCGATGCCGAAACAAACCTATTTATATCGCTTCTTACCACTTTCCTGATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGC‑GGGAA‑GTAGTTTTCGCCGGCAGCGTA‑GGC‑GTTAGGTTCATTGTGGGCGTAGCGGTATTCGGCGCCGAAGCCGAGGTCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGACGTCGAAATCGGC‑GGATTC‑GGTT‑G‑CCAATTTTTTG‑GCGGCTTTGAAAGCGAGATAAACGGCGTTGCTTTTCGGTGCGACGGCTAAATACACAATGGCTTGCGCAATAGCGCGTTCGCCTTCGGCAGCGCCGACGCGGG  >  1/1909870‑1910301                                                                                                                                                                                                |                                                                                                                                                                                                                                                          ggTACGAAGTGCAAGCGCAGCGCAGTAAAAGTAGGTCGATGCCGAAACAAACCTATTTATATCGCTTCTTACCACTTTCCTGATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGC‑GGGGA‑GTAGGTTTTGCCGGCCGCGTA‑GGC‑GTTTGGTTTATTTTGGGGGGAGCGGGATTCGGCGCCGCAGCCGAgg                                                                                                                                                                                            >  1:460247/1‑251 (MQ=255)         gTGCAAGCGCAGCGCAGTAAAAGTAGGTCGATGCCGAAACAAACCTATTTATATCGCTTCTTACCACTTTCCTGATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGC‑GGGAA‑GCAGTTTTTGCCGGCCGCGAC‑GGC‑GGTAGGTTCCTTGTGGGGGGAGCGGGATTTGGCGCCGAAGCCGAGGGCTTTCAt                                                                                                                                                                                    >  1:565392/1‑251 (MQ=255)               gcgcagcgcaGTAAAAGTAGGTCGATGCCGAAACAAACCTATTTATATCGCTTCTTACCACTTTCCTGATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGC‑GGGAA‑GTAGTTTTTGCCGGCGGCGGA‑GGC‑GTTTGGGTTTTTGTGGGGGGTGGGGGATTCGGCGCCGGAGCCGCGGGCTTTTATGacg                                                                                                                                                                                >  1:24725/1‑247 (MQ=255)                       cagTAAAAGTAGGTCGATGCCGAAACAAACCTATTTATATCGCTTCTTACCACTTTCCTGATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGC‑GGGGA‑GTAGTTTTTGCCGGGAGCGTA‑GGG‑GGTAGGGTCTTTGTGGGGGTTGCGGGATTTGGCGCCGGAGCCGGGGGCTTTCATGAAGCTGGTCgggg                                                                                                                                                                      >  1:630821/1‑251 (MQ=255)                       cagTAAAAGTAGGTCGATGCCGAAACAAACCTATTTATATCGCTTCTTACCACTTTCCTGATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGC‑GGGAA‑GGAATTTTTGCCGGCAGCGTT‑GGCGGTTGGGTTC‑TTGTGGGGGGAGCGGGGTTTGGCGCCGAAGGCCAGGGCTTTTAAGTAGGTGGTCgggg                                                                                                                                                                      >  1:5927/1‑251 (MQ=255)                             aaGTAGGTCGATGCCGAAACAAACCTATTTATATCGCTTCTTACCACTTTCCTGATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGC‑GGGAA‑GTAGGTTTCGCCGGCCGCGGA‑GGG‑GTTAGGGTCCTTGTGGGGGTAGCGGGATTTGGGGCCGCAGCCGAGGGCTTTCATGAAGCGGGGCGGGGCGTTgg                                                                                                                                                                >  1:183583/1‑250 (MQ=255)                                  ggTCGATGCCGAAACAAACCTATTTATATCGCTTCTTACCACTTTCCTGATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGC‑GGGAA‑GGAGGTTTTGCCGGCAGCGGA‑GGG‑GTTAGGGTCATTGTGGGGGGAGCGGGATTTGGCGCCGAAGCCGAGGGCTTTTATTAAGCTGGGCGGGGCGGTGCggggg                                                                                                                                                           >  1:279301/1‑248 (MQ=255)                                             aaacaaacCTATTTATATCGCTTCTTACCACTTTCCTGATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGC‑GGGAA‑GTAGTTTTCGCCGGCAGCGTA‑GGC‑GTTAGGTTCATTGTGGGCGTAGCGGTATTCGGCGCCGAAGCCGAGGTCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGa                                                                                                                                                 <  1:71662/250‑1 (MQ=255)                                                  aacCTATTTATATCGCTTCTTACCACTTTCCTGATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGC‑GGGAA‑GTAGTTTTCGCCGGCAGCGTA‑GGC‑GTTAGGTTCATTGTGGGCGTAGCGGTATTCGGCGCCGAAGCCGAGGTCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAggg                                                                                                                                                  <  1:626917/244‑1 (MQ=255)                                                       aTTTATATCGCTTCTTACCACTTTCCTGATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGC‑GGGAA‑GTAGGTTTTGCCGGCAGCGTAGGGG‑GTTGGGTT‑ATTGTGGGGGTAGCGGGATTCGGCGCCGAAGCCGAGGGCTTTCTTGAAGCTGGTCGGGGCGTTGCGGGGATGTTCAGGGGCGTCGGAATcg                                                                                                                                      >  1:503962/1‑251 (MQ=255)                                                                gCTTCTTACCACTTTCCTGATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGC‑GGGAA‑GTAGTTTTCGCCGGCAGCGGA‑GGC‑GTTAGGTTCATTGTGGGGGTAGCGGGATTTGGCGCCGAAGCCGAGGGCTTTTATGAAGCTGGTCGGGGCGTTGCGGGGATGTTCAGGGACGTCGAAATCGGC‑GGGTTC‑g                                                                                                                           >  1:471977/1‑251 (MQ=255)                                                                   tcttACCACTTTCCTGATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGC‑GGGAA‑GTAGTTTTTGCCGGCAGCGTA‑GGC‑GTTTGGGTTATTTTGGGGGGAGGGGGATTTGGCGCCGGAGCCGAGGGCTTTCCTGAAGCTGGGCGGGGGGGTGCGGGGATGTTTAGGGGCGGCGAAATcggc‑ggggttgggg                                                                                                                         >  1:463377/1‑243 (MQ=255)                                                                   tcttACCACTTTCCTGATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGC‑GGGAA‑GTAGGTTTTGCCGGCAGCGGA‑GGC‑GGTAGGGTCATTGTGGGCGTAGCGGGATTCGGCGCCGAAGCCGAGGGCTTTCATGAAGCTGGGCGGGGCGGTGCGGAGAGGTTCAGGGACGTCGAAATcggc‑gggtttgggt                                                                                                                         >  1:312269/1‑243 (MQ=255)                                                                   tattaacACTTTCCTGATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGC‑GGGAA‑GTAGTTTTTGCCGGCAGCGTA‑GGG‑GTTTGGGTTATTGTGGGGGTGGCGGGATTTGGGGCCGCAGCCGAGGGCTTTCATGAAGCTGGTCGGGGGGTTGCGGGGATGTTCAGGGGCGTCGAAATCGGC‑GGGTTT‑GGtt                                                                                                                        >  1:42473/7‑251 (MQ=255)                                                                             ttCCTGATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGGGAA‑G‑AGGTTTCGCCGGGAGGGTA‑GGC‑GTTTGGTTCATTTTGGGCGGCGCGGGTTTTGGGGCCGAAACCGGGGGCTTTTCTGAAGCTGGGCGGGGGGGTGGGGGGATGTTCTGGGGCGTCGAAATcggc‑ggggttcgttggcgattttt                                                                                                               >  1:624596/1‑233 (MQ=255)                                                                             ttCCTGATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGC‑GGGGA‑GGAGGTTTTGCCGGGCGCGGA‑GGC‑GTTTGGGTCATTGTTGGGGGAGGGGGATTTGGGGCCGGAGCCGAGGGCTTTCATGAAGCTGGGCGGGGGGGTGCGGGGGTGTTTAGGGGCGTCGAAATcggc‑ggggttgggtttgtcttttt                                                                                                               >  1:36701/1‑233 (MQ=255)                                                                             ttCCTGATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGC‑GGGAA‑GTAGTTTTTGCCGGCCGCGGA‑GGC‑GGTTGGGTCATTGTGGGGGTAGCGGGATTCGGCGCCGAAGCCGAGGGCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGGCGGCGAAATCGGC‑GGGTTCGGGTT‑G‑‑CAAttttt                                                                                                            >  1:428845/1‑251 (MQ=255)                                                                             ttCCTGATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGC‑GGGAA‑GGAGTTTTCGCCGGCAGCGTA‑GGC‑GTTTGGGTCATTGTGGGCGGAGCGGGATTCGGCGCCGAAGCCGCGGGCTTTCATGAAGCTGGGCGGGGCGGTGCGGGGAGGTTCAGGGGCGGCGAAATCGGC‑GGGTTT‑GGTT‑G‑GCCAttttt                                                                                                            >  1:430749/1‑251 (MQ=255)                                                                                  gATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGC‑GGGGA‑GGAGTTTTCGCCGGCCGCGGA‑GGC‑GGTTGGTTCATTGTGGGCGGAGGGGGATTTGGCGCCGAAGCCGAGGGCTTTTATGAAGCCGGGCGGGGGGGTGCGGGGGTGGTCAGGGGCGTCGAAAACCGCGGGGTTC‑GGTG‑G‑CCATTTTTTTg‑gg                                                                                                       >  1:415920/1‑250 (MQ=255)                                                                                  gATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGC‑GGGAA‑GTAGTTTTCGCCCGCAGCGGA‑GGG‑GGTAGGTTCATTTTGGGCGGAGCGGTATTTGGCGCCGAAGCCGAGGGCTTTTATGAAGCTGGTCGGGGCGTTGCGGGGATGTTCCGGGGCGTCGAAAAcggc‑ggggttgggtggccatttttttgcg                                                                                                          >  1:557344/1‑228 (MQ=255)                                                                                  gATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGC‑GGGAA‑GAAGGTTTTTCCCGCAGCGGA‑GGC‑GTTTGGTTCCTTGTGGGCGGAGCGGGTTTCGGCGCCCAAGCCGAGGGCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGAAGTTCAGGGGCGTCGAAATCGGC‑GGGTTC‑ggggggccatttttttgcg                                                                                                         >  1:473487/1‑234 (MQ=255)                                                                                   aTCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGC‑GGGAAGGTGGTTTT‑GCCGGCCACGCG‑GGC‑GTTTGGTTTATTGTGGGCGGAGCGGTATTCCGCGCCGAAGCCGAGGGCTTTCATGAAGCGGGTCGGGGGGTTGCGGGGATGTTCCAGGACGTCGAAATTGGC‑GGGGTC‑GGGT‑G‑GCAAATTTTTt‑gcgg                                                                                                     >  1:438556/1‑251 (MQ=255)                                                                                       aaCCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGC‑GGGGA‑GTAGTTTTTGCCGGCAGCGTA‑GGC‑GGTAGGGTCATTGTGGGCGTAGCGGGATTTGGCGCCGAAGCCGAGGGCTTTCATTAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGACGTCGAAATCGGC‑GGGGTC‑GGGT‑G‑CCAATTTTTTT‑GGGGCttt                                                                                                 >  1:125074/1‑251 (MQ=255)                                                                                            ccGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGC‑GGGAA‑GTAGTTTTCGCCGGCAGCGTA‑GGC‑GTTAGGTTCATTGTGGGCGTAGCGGTATTCGGCGCCGAAGCCGAGGTCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGACGTCGAAATCGGC‑GGATTC‑GGTT‑G‑CCAATTTTTTG‑GCGGCTTTGAAAg                                                                                            <  1:278616/251‑1 (MQ=255)                                                                                            ccGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGC‑GGGAA‑GTAGTTTTCGCCGGCAGCGTA‑GGC‑GTTAGGTTCATTGTGGGCGTAGCGGTATTCGGCGCCGAAGCCGAGGTCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGACGTCGAAATCGGC‑GGATTC‑GGTT‑G‑CCAATTTTTTG‑GCGGCTTTGAAAg                                                                                            <  1:60984/251‑1 (MQ=255)                                                                                                   cATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGC‑GGGAA‑GGAGTTTTCGCCGGCAGCGTA‑GGC‑GGTAGGTTCATTGTGGGGGGAGCGGGATTCGGCGCCGAAGCCGAGGGCTTTCATGAAGCTGGGCGGGGCGGTGCGGGGGTGTTCAGGGACGGCGAAAt                                                                                                                                        >  1:13260/1‑205 (MQ=255)                                                                                                   cATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGC‑GGGAA‑GGAGGTTTTGCCGGCAGCGGA‑GGG‑GGTAGGTTTCTTGTGGGCGTAGCGGGATTTGGCGCCGGAGCCGAGGGCTTTTATGAAGCTGGGCGGGGCGTTGCGGGGGTGTTTAGGGGCGGCGGAATCGGC‑GGGTTT‑GGGT‑GCCCAATTTTTTgcggcgttttaagggagaga                                                                                       >  1:641156/1‑232 (MQ=255)                                                                                                      tCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGC‑GGGAA‑GTAGTTTTCGCCGGCAGCGTA‑GGC‑GTTAGGTTCATTGTGGGCGTAGCGGTATTCGGCGCCGAAGCCGAGGTCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGACGTCGAAATCGGC‑GGATTC‑GGTT‑G‑CCAATTTTTTG‑GCGGCTTTGAAAGCGAGATAAAc                                                                                  <  1:502651/251‑1 (MQ=255)                                                                                                        cAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGC‑GGGAA‑GTAGTTTTCGCCGGCAGCGTA‑GGC‑GTTAGGTTCATTGTGGGCGTAGCGGTATTCGGCGCCGAAGCCGAGGTCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGACGTCGAAATCGGC‑GGATTC‑GGTT‑G‑CCAATTTTTTG‑GCGGCTTTGAAAGCGAGATAAACgg                                                                                <  1:17638/251‑1 (MQ=255)                                                                                                         aaCTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGC‑GGGAA‑GTAGTTTTTGCCGGCAGCGTA‑GGG‑GGTAGGGTCATTTTGGGCGGAGCGGGATTCGGCGCCGCAGACGAGGGCTTTCATGAAGCTGGTCGGGGCGGTGCGGAGATGTTCAGGGACGTCGAAATCGGC‑GGGATC‑GGGT‑G‑CCCCTTTTTTT‑GGGGCtttttaaaggggataaaagg                                                                                >  1:83069/1‑233 (MQ=255)                                                                                                                ccTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGC‑GGGGA‑GTAGTTTTTGCCGGCAGCGTA‑GGC‑GTTTGGTTCATTGTGGGCGGAGCGGGATTCGGCGCCGAAGCCGAGGGCTTTCATGAAGCTGGTCGGGGGGGTGCGGAGATGTTTAGGGACGGCGGAATCGGG‑GGGTTCGGGTG‑G‑CCATTTTTTTG‑GGGGGTTTG‑AAGGGAGATAAAAGGCGGTGCtt                                                                        >  1:368799/1‑251 (MQ=255)                                                                                                                ccTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGC‑GGGGA‑GTAGGTTTTGCCGGCAGCGTA‑GGG‑GGTAGGtttcttg                                                                                                                                                                                                                             >  1:617475/1‑102 (MQ=255)                                                                                                                       cttaatTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGC‑GGGAA‑GGAGGTTTTGCCGGCAGCGGA‑GGG‑GTTAGGGTCATTGTGGGGGGAGCGGGATTCGGGGCCGAAGCCGGGGGCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGGCGTCGAAATAGGC‑GGGTTT‑GGGT‑G‑GCAAATTTTTGTGGGGGTTTGAAA‑AGAGATAAAAGGCGGTGCTTTTTGGTg                                                                 >  1:628433/1‑251 (MQ=255)                                                                                                                             ttCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGC‑GGGAA‑GTAGTTTTCGCCGGCAGCGTA‑GGC‑GGTAGGTTCATTGTGGGGGTAGCGGTATTCGGCGCCGAAGCCGAGGGCTTTTATGAAGCTGGTCGGGGCGTTGCGGAGATGGTCAGGGGCGTCGAAATCGGC‑GGATTT‑GGGT‑G‑CCCATTTTTTT‑GCGGGCTTGAAAGCGGGATAAAAGGCGGTGCTTTTTGGTGcggcgg                                                           >  1:196515/1‑251 (MQ=255)                                                                                                                                     ctctGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGC‑GGGGA‑GGAGTTTTTGCCGGCCGCGGA‑GGC‑GGTTGGGTCATTGTGGGGGTAGCGGGATTCGGGGCCGGAGCCGGGGGCTTTTATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGACGTCGGAAt                                                                                                                                        >  1:405903/1‑171 (MQ=255)                                                                                                                                     ctctGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGC‑GGGAA‑GTAGTTTTCGCCGGCAGCGTA‑GGC‑GTTAGGTTCATTGTGGGCGTAGCGGTATTCGGCGCCGAAGCCGAGGTCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGACGTCGAAATCGGC‑GGATTC‑GGTT‑G‑CCAATTTTTTG‑GCGGCTTTGAAAGCGAGATAAACGGCGTTGCTTTTCGGTGCGACGGCTAAATac                                                   <  1:230797/251‑1 (MQ=255)                                                                                                                                     ctctGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGC‑GGGAA‑GTAGTTTTCGCCGGCAGCGTA‑GGC‑GTTAGGTTCATTGTGGGCGTAGCGGTATTCGGCGCCGAAGCCGAGGTCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGACGTCGAAATCGGC‑GGATTC‑GGTT‑G‑CCAATTTTTTG‑GCGGCTTTGAAAGCGAGATAAACGGCGTTGCTTTTCGGTGCGACGGCTAAATac                                                   <  1:255827/251‑1 (MQ=255)                                                                                                                                       ctGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGC‑GGGGA‑GTAGGTTTTGCCGGCAGCGTA‑GGG‑GTTAGGTTCATTGTGGGGGGAGCGGGATTTGGCGCCGAAGCCGAGGGCTTTCATGAAGCTGGTCGGGGGGTTGCGGGGGTGTTCAGGGGCGTCGGAAACGGC‑GGGTTC‑GGTTGG‑CCATTTTTTTG‑G‑GGCTTTTTAAAAGGGATAAAAGGCGGTGCTTTTTTGTGGGGCGGCTAAATaaaa                                                 >  1:510407/1‑248 (MQ=255)                                                                                                                                       ctGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGC‑GGGAA‑GTAGTTTTCGCCGGCAGCGTA‑GGC‑GTTAGGTTCATTGTGGGCGTAGCGGTATTCGGCGCCGAAGCCGAGGTCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGACGTCGAAATCGGC‑GGATTC‑GGTT‑G‑CCAATTTTTTG‑GCGGCTTTGAAAGCGAGATAAACGGCGTTGCTTTTCGGTGCGACGGCTAAATacac                                                 <  1:822/251‑1 (MQ=255)                                                                                                                                       ctGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGC‑GGGAA‑GTAGTTTTCGCCGGCAGCGTA‑GGC‑GTTAGGTTCATTGTGGGCGTAGCGGTATTCGGCGCCGAAGCCGAGGTCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGACGTCGAAATCGGC‑GGATTC‑GGTT‑G‑CCAATTTTTTG‑GCGGCTTTGAAAGCGAGATAAACGGCGTTGCTTTTCGGTGCGACGGCTAAATacac                                                 <  1:254164/251‑1 (MQ=255)                                                                                                                                        tggtggTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGC‑GGGAA‑GTAGTTTTCGCCGGCAGCGTA‑GGC‑GTTAGGTTCATTGTGGGCGTAGCGGTATTCGGCGCCGAAGCCGAGGTCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGACGTCGAAATCGGC‑GGATTC‑GGTT‑G‑CCAATTTTTTG‑GCGGCTTTGAAAGCGAGATAAACGGCGTTGCTTTTCGGTGCGACGGCTAAATAcaca                                                <  1:210631/251‑1 (MQ=255)                                                                                                                                           tggtCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGC‑GGGAA‑GTAGTTTTCGCCGGCAGCGTA‑GGC‑GTTAGGTTCATTGTGGGCGTAGCGGTATTCGGCGCCGAAGCCGAGGTCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGACGTCGAAATCGGC‑GGATTC‑GGTT‑G‑CCAATTTTTTG‑GCGGCTTTGAAAGCGAGATAAACGGCGTTGCTTTTCGGTGCGACGGCTAAATACACAAt                                              <  1:530893/250‑1 (MQ=255)                                                                                                                                           tggtCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGC‑GGGAA‑GTAGTTTTCGCCGGCAGCGTA‑GGC‑GTTAGGTTCATTGTGGGCGTAGCGGTATTCGGCGCCGAAGCCGAGGTCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGACGTCGAAATCGGC‑GGATTC‑GGTT‑G‑CCAATTTTTTG‑GCGGCTTTGAAAGCGAGATAAACGGCGTTGCTTTTCGGTGCGACGGCTAAATACACAAt                                              <  1:308066/250‑1 (MQ=255)                                                                                                                                              tCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGC‑GGGAA‑GTAGTTTTCGCCGGCAGCGTA‑GGC‑GTTAGGTTCATTGTGGGCGTAGCGGTATTCGGCGCCGAAGCCGAGGTCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGACGTCGAAATCGGC‑GGATTC‑GGTT‑G‑CCAATTTTTTG‑GCGGCTTTGAAAGCGAGATAAACGGCGTTGC‑TTTCGGTGCGACGGCTAAATACACAATGGCt                                          <  1:621160/250‑1 (MQ=255)                                                                                                                                                   aaaTAGAATTGGGTGTCTTTCAGTTGTGGC‑GGGAA‑GGAGTTTTTGCCCGCCGCGGA‑GGC‑GGTTGGGTCCTTGTGGGCGGAGCGGGATTCGGGGCCGGAGCCGAGGGCTTTCATGAAGCTGGTCGGGGGGTTGCGGGGGTGTTCCGGGGCGTCGGAAACGGC‑GGGGTCGGGTT‑C‑CCAATTTTTTG‑GGGGCTTTTAAA‑CGAGGAAAAAAGCGGTGCTTTTTGGTGCGGCGGGCCAATACACaaaaggctgtg                                      >  1:392409/1‑241 (MQ=255)                                                                                                                                                     aTAGAATTGGGTGTCTTTCAGTTGTGGC‑GGGAA‑GTAGTTTTCGCCGGCAGCGTA‑GGC‑GTTAGGTTCATTGTGGGCGTAGCGGTATTCGGCGCCGAAGCCGAGGTCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGACGTCGAAATCGGC‑GGATTC‑GGTT‑G‑CCAATTTTTTG‑GCGGCTTTGAAAGCGAGATAAACGGCGTTGCTTTTc                                                                     <  1:119623/217‑1 (MQ=255)                                                                                                                                                       aGAATTGGGTGTCTTTCAGTTGTGGC‑GGGAA‑GTAGTTTTCGCCGGCAGCGTA‑GGC‑GTTAGGTTCATTGTGGGCGTAGCGGTATTCGGCGCCGAAGCCGAGGTCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGACGTCGAAATCGGC‑GGATTC‑GGTT‑G‑CCAATTTTTTG‑GCGGCTTTGAAAGCGAGATAAACGGCGTTGCTTTTCGGTGCGACGGCTAAATACACAATgg                                            <  1:56234/240‑1 (MQ=255)                                                                                                                                                         aaTTGGGTGTCTTTCAGTTGTGGC‑GGGAA‑GTAGTTTTCGCCGGCAGCGTA‑GGC‑GTTAGGTTCATTGTGGGCGTAGCGGTATTCGGCGCCGAAGCCGAGGTCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGACGTCGAAATCGGC‑GGATTC‑GGTT‑G‑CCAATTTTTTG‑GCGGCTTTGAAAGCGAGATAAACGGCGTTGCTTTTCGGTGCGACGGCTAAATACACAATGGCTTGCGCAATAg                                <  1:409333/250‑1 (MQ=255)                                                                                                                                                         aaTTGGGTGTCTTTCAGTTGTGGC‑GGGAA‑GTAGTTTTCGCCGGCAGCGTA‑GGC‑GTTAGGTTCATTGTGGGCGTAGCGGTATTCGGCGCCGAAGCCGAGGTCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGACGTCGAAATCGGC‑GGATTC‑GGTT‑G‑CCAATTTTTTG‑GCGGCTTTGAAAGCGAGATAAACGGCGTTGCTTTTCGGTGCGACGGCTAAATACACAATGGCTTGCGCAATAg                                <  1:462041/250‑1 (MQ=255)                                                                                                                                                         aaTTGGGTGTCTTTCAGTTGTGGC‑GGGAA‑GTAGTTTTCGCCGGCAGCGTA‑GGC‑GTTAGGTTCATTGTGGGCGTAGCGGTATTCGGCGCCGAAGCCGAGGTCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGACGTCGAAATCGGC‑GGATTC‑GGTT‑G‑CCAATTTTTTG‑GCGGCTTTGAAAGCGAGATAAACGGCGTTGCTTTTCGGTGCGACGGCTAAATACACAATGGCTTGCGCAATAg                                <  1:223907/250‑1 (MQ=255)                                                                                                                                                          aTTGGGTGTCTTTCAGTTGTGGC‑GGGAA‑GTAGTTTTCGCCGGCAGCGTA‑GGC‑GTTAGGTTCATTGTGGGCGTAGCGGTATTCGGCGCCGAAGCCGAGGTCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGACGTCGAAATCGGC‑GGATTC‑GGTT‑G‑CCAATTTTTTG‑GCGGCTTTGAAAGCGAGATAAACGGCGTTGCTTTTCGGTGCGACGGCTAAATACACAAt                                              <  1:523959/235‑1 (MQ=255)                                                                                                                                                                                    gAA‑GTAGTTTTCGCCGGCAGCGTA‑GGC‑GTTAGGTTCATTGTGGGCGTAGCGGTATTCGGCGCCGAAGCCGAGGTCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGACGTCGAAATCGGC‑GGATTC‑GGTT‑G‑CCAATTTTTTG‑GCGGCTTTGAAAGCGAGATAAACGGCGTTGCTTTTCGGTGCGACGGCTAAATACACAATGGCTTGCGCAATAGCGCGTTCGCCTTCGGCAGCGCCGAcg      >  1:209445/1‑250 (MQ=255)                                                                                                                                                                                        gtagtTTTCGCCGGCAGCGTA‑GGC‑GTTAGGTTCATTGTGGGCGTAGCGGTATTCGGCGCCGAAGCCGAGGTCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGACGTCGAAATCGGC‑GGATTC‑GGTT‑G‑CCAATTTTTTG‑GCGGCTTTGAAAGCGAGATAAACGGCGTTGCTTTTCGGTGCGACGGCTAAATACACAATGGCTTGCGCAATAGCGCGTTCGCCTTCGGCAGCGCCGACGCggg  >  1:596548/1‑251 (MQ=255)                                                                                                                                                                                          agtTTTCGCCGGCAGCGTA‑GGC‑GTTAGGTTCATTGTGGGCGTAGCGGTATTCGGCGCCGAAGCCGAGGTCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGACGTCGAAATCGGC‑GGATTC‑GGTT‑G‑CCAATTTTTTG‑GCGGCTTTGAAAGCGAGATAAACGGCGTTGCTTTTCGGTGCGACGGCTAAATACACAATGGCTTGCGCAATAg                                >  1:145740/1‑219 (MQ=255)                                                                                                                                                                                                |                                                                                                                                                                                                                                                          GGTACGAAGTGCAAGCGCAGCGCAGTAAAAGTAGGTCGATGCCGAAACAAACCTATTTATATCGCTTCTTACCACTTTCCTGATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGC‑GGGAA‑GTAGTTTTCGCCGGCAGCGTA‑GGC‑GTTAGGTTCATTGTGGGCGTAGCGGTATTCGGCGCCGAAGCCGAGGTCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGACGTCGAAATCGGC‑GGATTC‑GGTT‑G‑CCAATTTTTTG‑GCGGCTTTGAAAGCGAGATAAACGGCGTTGCTTTTCGGTGCGACGGCTAAATACACAATGGCTTGCGCAATAGCGCGTTCGCCTTCGGCAGCGCCGACGCGGG  >  1/1909870‑1910301

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑