Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*11,866,5260TG40.5% 66.4 / 12.0 42T614P (ACC→CCC) unknown[NiFe] hydrogenase metallocenter assembly protein HypF
Reads supporting (aligned to +/- strand):  ref base T (3/22);  new base G (16/1);  total (19/23)
Fisher's exact test for biased strand distribution p-value = 8.82e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GCGAGAATGGCGAAGCCTTCAGCCAACGCGTAATGGAAGATAAAGGCTTTGTCGGCCGCGGAGGCGTTGAGGGCGAGCCAGGTTTGCCAGAAGACGGCGAGATCTAATTTATTTTCATTGTTCAACGGCATTAAGATTGGAAGGTCGATTTCGTTTAATGGCAAACGGGCGAGTGTCGACTGTTCGGCGAGCACTTCTAATTGGCAGGCGGCTTCGCCTTCCCAGCTGAGTTGTGGCGGGGTGATGCCTAAGC‑CGAACGCCACGGCGTCGAAGAAGCGTCCGGCGGAAGAAATCAACGGGCTGTTGATTTTGCGTTCAATGGCGGTGGCGAGAAGTTGCCAGTCGTGATCGGCGCAGGTTTCGGCGACGAGCTCCCGCCAATTTGGCACAAATTGTTGTAAATGCGCCAGCCAGTTGCGCCAAGGCTGTTTGGCGGCAAGATCGCCGCCGGGCAGAGCGACGGCAGGTAAGCCGCCTAAATAGCGCGAGGC  >  1/1866285‑1866775
                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                          
gCGAGAATGGCGAAGCCTTCAGCCAACGCGTAATGGAAGATAAAGGCTTTGTCGGCCGCGGAGGCGTTGAGGGCGAGCCAGGTTTGCCAGAAGACGGCGAGATCTAATTTATTTTCATTGTTCAACGGCATTAAGATTGGAAGGTCGATTTCGTTTAATGGCAAACGGGCGAGTGTCGACTGTTCGGCGAGCACTTCTAATTGGCAGGCGGCTTCGCCTTCCCAGCTGAGTTGTGGCGGGGTGATGCCt                                                                                                                                                                                                                                                     <  1:249259/249‑1 (MQ=255)
     aaTGGCGAAGCCTTCAGCCAACGCGTAATGGAAGATAAAGGCTTTGTCGGCCGCGGAGGCGTTGAGGGCGAGCCAGGTTTGCCAGAAGACGGCGAGATCTAATTTATTTTCATTGTTCAACGGCATTAAGATTGGAAGGTCGATTTCGTTTAATGGCAAACGGGCGAGTGTCGACTGTTCGGCGAGCACTTCTAATTGGCAGGCGGCTTCGCCTTCCCAGCTGAGTTGTGGCGGGGTGATGCCTAAGc‑c                                                                                                                                                                                                                                               <  1:377407/249‑1 (MQ=255)
     aaTGGCGAAGCCTTCAGCCAACGCGTAATGGAAGATAAAGGCTTTGTCGGCCGCGGAGGCGTTGAGGGCGAGCCAGGTTTGCCAGAAGACGGCGAGATCTAATTTATTTTCATTGTTCAACGGCATTAAGATTGGAAGGTCGATTTCGTTTAATGGCAAACGGGCGAGTGTCGACTGTTCGGCGAGCACTTCTAATTGGCAGGCGGCTTCGCCTTCCCAGCTGAGTTGTGGCGGGGTGATGCCTAAGC‑Cg                                                                                                                                                                                                                                              <  1:627946/250‑1 (MQ=255)
     aaTGGCGAAGCCTTCAGCCAACGCGTAATGGAAGATAAAGGCTTTGTCGGCCGCGGAGGCGTTGAGGGCGAGCCAGGTTTGCCAGAAGACGGCGAGATCTAATTTATTTTCATTGTTCAACGGCATTAAGATTGGAAGGTCGATTTCGTTTAATGGCAAACGGGCGAGTGTCGACTGTTCGGCGAGCACTTCTAATTGGCAGGCGGCTTCGCCTTCCCAGCTGAGTTGTGGCGGGGTGATGCCTAAGC‑Cg                                                                                                                                                                                                                                              <  1:68283/250‑1 (MQ=255)
          cGAAGCCTTCAGCCAACGCGTAATGGAAGATAAAGGCTTTGTCGGCCGCGGAGGCGTTGAGGGCGAGCCAGGTTTGCCAGAAGACGGCGAGATCTAATTTATTTTCATTGTTCAACGGCATTAAGATTGGAAGGTCGATTTCGTTTAATGGCAAACGGGCGAGTGTCGACTGTTCGGCGAGCACTTCTAATTGGCAGGCGGCTTCGCCTTCCCAGCTGAGTTGTGGCGGGGTGATGCCTAAGC‑CGAACGc                                                                                                                                                                                                                                         <  1:212011/250‑1 (MQ=255)
              gCCTTCAGCCAACGCGTAATGGAAGATAAAGGCTTTGTCGGCCGCGGAGGCGTTGAGGGCGAGCCAGGTTTGCCAGAAGACGGCGAGATCTAATTTATTTTCATTGTTCAACGGCATTAAGATTGGAAGGTCGATTTCGTTTAATGGCAAACGGGCGAGTGTCGACTGTTCGGCGAGCACTTCTAATTGGCAGGCGGCTTCGCCTTCCCAGCTGAGTTGTGGCGGGGGGATGCCTAAGC‑CGAACGCCCCgg                                                                                                                                                                                                                                    >  1:300642/1‑251 (MQ=255)
               ccTTCAGCCAACGCGTAATGGAAGATAAAGGCTTTGTCGGCCGCGGAGGCGTTGAGGGCGAGCCAGGTTTGCCAGAAGACGGCGAGATCTAATTTATTTTCATTGTTCAACGGCATTAAGATTGGAAGGTCGATTTCGTTTAATGGCAAACGGGCGAGTGTCGACTGTTCGGCGAGCACTTCTAATTGGCAGGCGGCTTCGCCTTCCCAGCTGAGTTGTGGCGGGGGGGTGCCTAAGC‑CGGACGCCAccgg                                                                                                                                                                                                                                   >  1:463016/1‑248 (MQ=255)
                      ccAACGCGTAATGGAAGATAAAGGCTTTGTCGGCCGCGGAGGCGTTGAGGGCGAGCCAGGTTTGCCAGAAGACGGCGAGATCTAATTTATTTTCATTGTTCAACGGCATTAAGATTGGAAGGTCGATTTCGTTTAATGGCAAACGGGCGAGTGTCGACTGTTCGGCGAGCACTTCTAATTGGCAGGCGGCTTCGCCTTCCCAGCTGAGTTGTGGCGGGGTGATGCCTAAGC‑CGAACGCCACGGCGTCgaag                                                                                                                                                                                                                            <  1:590127/251‑1 (MQ=255)
                           gcgTAATGGAAGATAAAGGCTTTGTCGGCCGCGGAGGCGTTGAGGGCGAGCCAGGTTTGCCAGAAGACGGCGAGATCTACTTTATTTTCATTGTTCAACGGCATTAAGATTGGAAGGTCGATTTCGTTTAATGGCAAACGGGCGAGTGTCGACTGTTCGGCGAGCACTTCTAATTGGCAGGCGGCTTCGCCTTCCCAGCTGAGTTGTGGCGGGGGGGTGGCTAAAC‑CGAACGCCCCGGCGGCGgagaagag                                                                                                                                                                                                                       >  1:260171/1‑249 (MQ=255)
                                aTGGAAGATAAAGGCTTTGTCGGCCGCGGAGGCGTTGAGGGCGAGCCAGGTTTGCCAGAAGACGGCGAGATCTAATTTATTTTCATTGTTCAACGGCATTAAGATTGGAAGGTCGATTTCGTTTAATGGCAAACGGGCGAGTGTCGACTGTTCGGCGAGCACTTCTAATTGGCAGGCGGCTTCGCCTTCCCAGCTGAGTTGTGGCGGGGGGGTGCCTAAGC‑CGAACGCCACCGCGTCGGAGAAGCGTCcgg                                                                                                                                                                                                                  >  1:573248/1‑251 (MQ=255)
                                aTGGAAGATAAAGGCTTTGTCGGCCGCGGAGGCGTTGAGGGCGAGCCAGGTTTGCCAGAAGACGGCGAGATCTAATTTATTTTCATTGTTCAACGGCATTAAGATTGGAAGGTCGATTTCGTTTAATGGCAAACGGGCGAGTGTCGACTGTTCGGCGAGCACTTCTAATTGGCAGGCGGCTTCGCCTTCCCAGCTGAGTTGTGGCGGGGGGGTGCCTAAAC‑CGAACGCCACCGCGGCGAAGAAGCGGCcgg                                                                                                                                                                                                                  >  1:206547/1‑251 (MQ=255)
                                aTGGAAGATAAAGGCTTTGTCGGCCGCGGAGGCGTTGAGGGCGAGCCAGGTTTGCCAGAAGACGGCGAGATCTAATTTATTTTCATTGTTCAACGGCATTAAGATTGGAAGGTCGATTTCGTTTAATGGCAAACGGGCGAGTGTCGACTGTTCGGCGAGCACTTCTAATTGGCAGGCGGCTTCGCCTTCCCAGCTGAGTTGTGGCGGGGGGAAGGCCAAAC‑CGAACGcccaggccgcgtagaagagtccgg                                                                                                                                                                                                                  >  1:278031/1‑229 (MQ=255)
                                      gATAAAGGCTTTGTCGGCCGCGGAGGCGTTGAGGGCGAGCCAGGTTTGCCAGAAGACGGCGAGATCTAATTTATTTTCATTGTTCAACGGCATTAAGATTGGAAGGTCGATTTCGTTTAATGGCAAACGGGCGAGTGTCGACTGTTCGGCGAGCACTTCTAATTGGCAGGCGGCTTCGCCTTCCCAGCTGAGTTGTGGCGGGGGGGTGCCCAAGC‑CGGACGCCCCCGCGGCGGAAAAACGTCcggcgggag                                                                                                                                                                                                            >  1:12239/1‑248 (MQ=255)
                                      gATAAAGGCTTTGTCGGCCGCGGAGGCGTTGAGGGCGAGCCAGGTTTGCCAGAAGACGGCGAGATCTAATTTATTTTCATTGTTCAACGGCATTAAGATTGGAAGGTCGATTTCGTTTAATGGCAAACGGGCGAGTGTCGACTGTTCGGCGAGCACTTCTAATTGGCAGGCGGCTTCGCCTTCCCAGCTGAGTTGTGGCGGGGGGATGCCCTAGC‑CGAACGCCCCCGCGGCgaagaaagggccggcgggag                                                                                                                                                                                                            >  1:65671/1‑237 (MQ=255)
                                       aTAAAGGCTTTGTCGGCCGCGGAGGCGTTGAGGGCGAGCCAGGTTTGCCAGAAGACGGCGAGATCTAATTTATTTTCATTGTTCAACGGCATTAAGATTGGAAGGTCGATTTCGTTTAATGGCAAACGGGCGAGTGTCGACTGTTCGGCGAGCACTTCTAATTGGCAGGCGGCTTCGCCTTCCCAGCTGAGTTGTGGCGGGGGGATGCCTAAGC‑CGAACGCCACGGCGGCGGAGAAGCGTCCGGCGggaga                                                                                                                                                                                                           >  1:478448/1‑251 (MQ=255)
                                                              ggCGTTGAGGGCGAGCCAGGTTTGCCAGAAGACGGCGAGATCTAATTTATTTTCATTGTTCAACGGCATTAAGATTGGAAGGTCGATTTCGTTTAATGGCAAACGGGCGAGTGTCGACTGTTCGGCGAGCACTTCTAATTGGCAGGCGGCTTCGCCTTCCCAGCTGAGTTGTGGCGGGGGGATGCCTAAGCACG‑ACGCCCAGCCGCCGGAGCAGAGTCCGGCGGGAGAAATCAACAGGCGGtttgttttgg                                                                                                                                                                                    >  1:261644/1‑243 (MQ=255)
                                                               gCGTTGAGGGCGAGCCAGGTTTGCCAGAAGACGGCGAGATCTAATTTATTTTCATTGTTCAACGGCATTAAGATTGGAAGGTCGATTTCGTTTAATGGCAAACGGGCGAGTGTCGACTGTTCGGCGAGCACTTCTAATTGGCAGGCGGCTTCGCCTTCCCAGCTGAGTTGTGGCGGGGTGATGCCTAAGC‑CGAACGCCACGGCGTCGAAGAAGCGTCCGGCGGAAGAAATCAACGGGCTGTTGATTTTg                                                                                                                                                                                     <  1:87203/249‑1 (MQ=255)
                                                                cGTTGAGGGCGAGCCAGGTTTGCCAGAAGACGGCGAGATCTAATTTATTTTCATTGTTCAACGGCATTAAGATTGGAAGGTCGATTTCGTTTAATGGCAAACGGGCGAGTGTCGACTGTTCGGCGAGCACTTCTAATTGGCAGGCGGCTTCGCCTTCCCAGCTGAGTTGTGGCGGGGGGAGGCCCAAGC‑CGGACGCCCCGGCGGCGGAGAAGCGGCCGGGGGGAGAAAACAAAgggggggtggttttgggg                                                                                                                                                                                  >  1:457941/1‑236 (MQ=255)
                                                                           aGCCAGGTTTGCCAGAAGACGGCGAGATCTAATTTATTTTCATTGTTCAACGGCATTAAGATTGGAAGGTCGATTTCGTTTAATGGCAAACGGGCGAGTGTCGACTGTTCGGCGAGCACTTCTAATTGGCAGGCGGCTTCGCCTTCCCAGCTGAGTTGTGGCGGGGGGAGGCCCAAGC‑CGAACGCCCCCGCGGCCGAGAAGGGGCCGGGGGGAGAAATCAACGGGCGGTTTGTTTTTCGGTCAATTGCggg                                                                                                                                                                       >  1:632659/1‑250 (MQ=255)
                                                                                ggTTTGCCAGAAGACGGCGAGATCTAATTTATTTTCATTGTTCAACGGCATTAAGATTGGAAGGTCGATTTCGTTTAATGGCAAACGGGCGAGTGTCGACTGTTCGGCGAGCACTTCTAATTGGCAGGCGGCTTCGCCTTCCCAGCTGAGTTGTGGCGGGGGGATGGCTCAGC‑CGCACGCCCCCGCGGCCAAGAAGCGGCCGGCGGAAGAAATAAACCGGCGGTTGATTTTGCGGTCAAAGGCGGTggggg                                                                                                                                                                  >  1:259900/1‑248 (MQ=255)
                                                                                    tGCCAGAAGACGGCGAGATCTAATTTATTTTCATTGTTCAACGGCATTAAGATTGGAAGGTCGATTTCGTTTAATGGCAAACGGGCGAGTGTCGACTGTTCGGCGAGCACTTCTAATTGGCAGGCGGCTTCGCCTTCCCAGCTGAGTTGTGGCGGGGTGATGCCTAAGC‑CGAACGCCACGGCGTCGAAGAAGCGTCCGGCGGAAGAAATCAACGGGCTGTTGATGTTGCGTTCAATGGCGGTGGCGAGAAg                                                                                                                                                              <  1:187884/251‑1 (MQ=255)
                                                                                    tGCCAGAAGACGGCGAGATCTAATTTATTTTCATTGTTCAACGGCATTAAGATTGGAAGGTCGATTTCGTTTAATGGCAAACGGGCGAGTGTCGACTGTTCGGCGAGCACTTCTAATTGGCAGGCGGCTTCGCCTTCCCAGCTGAGTTGTGGCGGGGGGATGGCTAAGC‑CGAACGCCACGGCGTCGGAGAAGCGGCCGGCGGGAGAAAACAACGGGGTGTTGGtttttgggtccatgggggggggggggag                                                                                                                                                              >  1:103378/1‑227 (MQ=255)
                                                                                              cGGCGAGATCTAATTTATTTTCATTGTTCAACGGCATTAAGATTGGAAGGTCGATTTCGTTTAATGGCAAACGGGCGAGTGTCGACTGTTCGGCGAGCACTTCTAATTGGCAGGCGGCTTCGCCTTCCCAGCTGAGTTGTGGCGGGGGGATGGCTAAAC‑CGAACGCCCCGGCGGCGCAGAAGCGGCCGGCGGGAGAAAAAAACAGGGTGGTGGTTTTTCGTTTAATGGCggggggggggaggtggccgtcg                                                                                                                                                    >  1:512215/1‑231 (MQ=255)
                                                                                                     aTCTAATTTATTTTCATTGTTCAACGGCATTAAGATTGGAAGGTCGATTTCGTTTAATGGCAAACGGGCGAGTGTCGACTGTTCGGCGAGCACTTCTAATTGGCAGGCGGCTTCGCCTTCCCAGCTGAGTTGTGGCGGGGTGATGCCTAAGC‑CGAACGCCACGGCGTCGAAGAAGCGTCCGGCGGAAGAAATCAACGGGCTGTTGATTTTGCGTTCAATGGCGGTGGCGAGAAGTTGCCAGTCGTGATc                                                                                                                                               <  1:30641/249‑1 (MQ=255)
                                                                                                     aTCTAATTTATTTTCATTGTTCAACGGCATTAAGATTGGAAGGTCGATTTCGTTTAATGGCAAACGGGCGAGTGTCGACTGTTCGGCGAGCACTTCTAATTGGCAGGCGGCTTCGCCTTCCCAGCTGAGTTGTGGCGGGGTGATGCCTAAGC‑CGAACGCCACGGCGTCGAAGAAGCGTCCGGCGGAAGAAATCAACGGGCTGTTGATTTTGCGTTCAATGGCGGTGGCGAGAAGTTGCCAGTCGTGATCgg                                                                                                                                             <  1:179160/251‑1 (MQ=255)
                                                                                                                       gTTCAACGGCATTAAGATTGGAAGGTCGATTTCGTTTAATGGCAAACGGGCGAGTGTCGACTGTTCGGCGAGCACTTCTAATTGGCAGGCGGCTTCGCCTTCCCAGCTGAGTTGTGGCGGGGGGGTGCCTCAAC‑CGGACGCCACCGCGTCGAAGAAAGGTCCCGCGGGAGAAATCAACAGGCTGGTGATTTTTCGTTTAATGGCGGTGGGGGGGAGGTGGCAGTCGTGGTCggggcgggggttgggggcgg                                                                                                                           >  1:558885/1‑233 (MQ=255)
                                                                                                                               gCATTAAGATTGGAAGGTCGATTTCGTTTAATGGCAAACGGGCGAGTGTCGACTGTTCGGCGAGCACTTCTAATTGGCAGGCGGCTTCGCCTTCCCAGCTGAGTTGTGGCGGGGTGATGCCTAAGC‑CGAACGCCACGGCGTCGAAGAAGCGTCCGGCGGAAGAAATCAACGGGCTGTTGATTTTGCGTTCAATGGCGGTGGCGAGAAg                                                                                                                                                              <  1:245685/208‑1 (MQ=255)
                                                                                                                                       aTTGGAAGGTCGATTTCGTTTAATGGCAAACGGGCGAGTGTCGACTGTTCGGCGAGCACTTCTAATTGGCAGGCGGCTTCGCCTTCCCAGCTGAGTTGTGGCGGGGTGATGCCTAAGC‑CGAACGCCACGGCGTCGAAGAAGCGTCCGGCGGAAGAAATCAACGGGCTGTTGATTTTGCGTTCAATGGCGGTGGCGAGAAGTTGCCAGTCGTGATCGGCGCAGGTTTCGGCGACGAGCTCCCGCCAAttt                                                                                                             <  1:272960/249‑1 (MQ=255)
                                                                                                                                                                             tgtCGACTGTTCGGCGAGCACTTCTAATTGGCAGGCGGCTTCGCCTTCCCAGCTGAGTTGTGGCGGGGTGATGCCTAAGC‑CGAACGCCACGGCGTCGAAGAAGCGTCCGGCGGAAGAAATCAACGGGCTGTTGATTTTGCGTTCAATGGCGGTGGCGAGAAGTTGCCAGTCGTGATCGGCGCAGGTTTCGGCGACGAGCTCCCGCCAATTTGGCACAAATTGTTGTAAATGCGCCAGCCAGTTGCGCCa                                                                       <  1:199800/249‑1 (MQ=255)
                                                                                                                                                                               tCGACTGTTCGGCGAGCACTTCTAATTGGCAGGCGGCTTCGCCTTCCCAGCTGAGTTGTGGCGGGGTGATGCCTAAGC‑CGAACGCCACGGCGTCGAAGAAGCGTCCGGCGGAAGAAATCAACGGGCTGTTGATTTTGCGTTCAATGGCGGTGGCGAGAAGTTGCCAGTCGTGATCGGCGCAGGTTTCGGCGACGAGCTCCCGCCAATTTGGCACAAATTGTTGTAAATGCGCCAGCCAGTTGCGCCAAGGc                                                                   <  1:112893/251‑1 (MQ=255)
                                                                                                                                                                                            gAGCACTTCTAATTGGCAGGCGGCTTCGCCTTCCCAGCTGAGTTGTGGCGGGGTGATGCCTAAGC‑CGAACGCCACGGCGTCGAAGAAGCGTCCGGCGGAAGAAATCAACGGGCTGTTGATTTTGCGTTCAATGGCGGTGGCGAGAAGTTGCCAGTCGTGATCGGCGCAGGTTTCGGCGACGAGCTCCCGCCAATTTGGCACAAATTGTTGTAAATGCGCCAGCCAGTTGCGCCAAGGCTGTTTGGCGGCa                                                       <  1:475048/250‑1 (MQ=255)
                                                                                                                                                                                                 cTTCTAATTGGCAGGCGGCTTCGCCTTCCCAGCTGAGTTGTGGCGGGGTGATGCCTAAGC‑CGAACGCCACGGCGTCGAAGAAGCGTCCGGCGGAAGAAATCAACGGGCTGTTGATTTTGCGTTCAATGGCGGTGGCGAGAAGTTGCCAGTCGTGATCGGCGCAGGTTTCGGCGACGAGCTCCCGCCAATTTGGCACAAATTGTTGTAAATGCGCCAGCCAGTTGCGCCAAGGc                                                                   <  1:496980/233‑1 (MQ=255)
                                                                                                                                                                                                                     tCGCCTTCCCAGCTGAGTTGTGGCGGGGTGATGCCTAAGC‑CGACCGCCACGGCGTCGAAGAAGCGTCCGGCGGAAGAAATCAACGGGCTGTTGATTTTGCGTTCAATGGCGGTGGCGAGAAGTTGCCAGTCGTGATCGGCGCAGGTTGCGGCGACGAGCTCCCGCCAATTTGGCACAAATTGTTGTAAATGCGCCAGCCAGTTGCGCCAAGGCTGTTTGGCGGCAAGATCGCCGCCGGGCAGAGCGACGGc                             <  1:643003/251‑1 (MQ=255)
                                                                                                                                                                                                                        ccTTCCCAGCTGAGTTGTGGCGGGGTGATGCCTAAGC‑CGAACGCCACGGCGTCGAAGAAGCGTCCGGCGGAAGAAATCAACGGGCTGTTGATTTTGCGTTCAATGGCGGTGGCGAGAAGTTGCCAGTCGTGATCGGCGCAGGTTTCGGCGACGAGCTCCCGCCAATTTGGCACAAATTGTTGTAAAt                                                                                          <  1:100856/187‑1 (MQ=255)
                                                                                                                                                                                                                        ccTTCCCAGCTGAGTGGTGGCGGGGGGATGCCTAAGC‑CGAACGCCACGGCGTCGAAGAAGCGTCCGGCGGAAGAAATCAACGGGCTGTTGATTTTGCGTTCAATGGCGGTGGCGAGAAGTGGCCAGTCGTGATCGGCGCAGGTTTCGGCGACGAGCTCCCGCCAATTTGGCACAAATTGTTGTAAATGCGCCAGCCAGTTGCGCCAAGGCTGTTTGGCGGCAAGATCGCCGCCGGGCAGAGCGACGGCAgg                          <  1:547569/251‑1 (MQ=255)
                                                                                                                                                                                                                           tCCCAGCGGAGTGGTGGCGGGGTGATGCCTAAGC‑CGAACGCCACGGCGTCGAAGAAGCGTCCGGCGGAAGAAATCAACGGGCTGTTGATTTTGCGTTCAATGGCGGTGGCGAGAAGTTGCCAGTCGTGATCGGCGCAGGTTTCGGCGACGAGCTCCCGCCAATTTGGCACAAATTGTTGTAAATGCGCCAGCCAGTTGCGCCAAGGCTGTTTGGCGGCAAGATCGCCGCCGGGCAGAGc                                   <  1:253419/239‑1 (MQ=255)
                                                                                                                                                                                                                                      ttGTGGCGGGGTGATGCCTAAGC‑CGAACGCCACGGCGTCGAAGAAGCGTCCGGCGGAAGAAATCAACGGGCTGTTGATTTTGCGTTCAATGGCGGTGGCGAGAAGTTGCCAGTCGTGATCGGCGCAGGTTTCGGCGACGAGCTCCCGCCAATTTGGCACAAATTGTTGTAAATGCGCCAGCCAGTTGCGCCAAGGCTGTTTGGCGGCAAGATCGCCGCCGGGCAGAGCGACGGCAGGTAAGCCGCCTAAAt            <  1:101043/251‑1 (MQ=255)
                                                                                                                                                                                                                                      tgggggCGGGGTGATGCCTAAGC‑CGAACGCCACGGCGTCGAAGAAGCGTCCGGCGGAAGAAATCAACGGGCTGTTGATTTTGCGTTCAATGGCGGTGGCGAGAAGTTGCCAGTCGTGATCGGCGCAGGTTTCGGCGACGAGCTCCCGCCAATTTGGCACAAATTGTTGTAAATGCGCCAGCCAGTTGCGCCAAGGCTGTTTGGCGGCAAGATCGCCGCCGGGCAGAGCGACGGCAGGTAAGCCGCCTAAAt            <  1:88444/247‑1 (MQ=255)
                                                                                                                                                                                                                                         tgGCGGGGTGATGCCTAAGC‑CGAACGCCACGGCGTCGAAGAAGCGTCCGGCGGAAGAAATCAACGGGCTGTTGATTTTGCGTTCAAGGGCGGTGGCGAGAAGTTGCCAGTCGTGATCGGCGCAGGTTTCGGCGACGAGCTCCCGCCAATTTGGCACAAATTGTTGTAAATGCGCCAGCCAGTTGCGCCAAGGCTGTTTGGCGGCAAGATCGCCGCCGGGCAGAGCGACGGCAGGTAAGCCGCCTAAATAg          <  1:264849/250‑1 (MQ=255)
                                                                                                                                                                                                                                               ggTGATGCCTAAGC‑CGAACGCCACGGCGTCGAAGAAGCGTCCGGCGGAAGAAATCAACGGGCTGTTGATTTTGCGTTCAATGGCGGTGGCGAGAAGTTGCCAGTCGTGATCGGCGCAGGTTTCGGCGACGAGCTCCCGCCAATTTGGCACAAATTGTTGTAAATGCGCCAGCCAGTTGCGCCAAGGCTGTTTTGCGGCAAGATCGCCGCCGGGCAGAGCGACGGCAGGTAAGCCGCCTAAATAGCGCGAgg   >  1:463085/1‑251 (MQ=255)
                                                                                                                                                                                                                                               ggTGATGCCTAAGC‑CGAACGCCACGGCGTCGAAGAAGCGTCCGGCGGAAGAAATCAACGGGCTGTTGATTTTGCGTTCAATGGCGGTGGCGAGAAGTTGCCAGTCGTGATCGGCGCAGGTTTCGGCGACGAGCTCCCGCCAATTTGGCACAAATTGTTGTAAATGCGCCAGCCAGTTGCGCCAAGGCTGTTTGGCGGCAAGATCGCCGCCGGGCAGAGCGACGGCAGGGAAGCCGCCTAAATAGCGCGAgg   >  1:610428/1‑251 (MQ=255)
                                                                                                                                                                                                                                                gTGATGCCTAAGC‑CGAACGCCACGGCGTCGAAGAAGCGTCCGGCGGAAGAAATCAACGGGCTGTTGATTTTGCGTTCAATGGCGGTGGCGAGAAGTTGCCAGTCGTGATCGGCGCAGGTTTCGGCGACGAGCTCCCGCCAATTTGGCACAAATTGTTGTAAATGCGCCAGCCAGTTGCGCCAAGGCTGTTTGGCGGCAAGATCGCCGCCGGGCAGAGCGACGGCAGGTAAGCCGCCTAAATAGGGCGAgg   >  1:488526/1‑250 (MQ=255)
                                                                                                                                                                                                                                                 tGATGCCTAAGC‑CGAACGCCACGGCGTCGAAGAAGCGTCCGGCGGAAGAAATCAACGGGCTGTTGATTTTGCGTTCAATGGCGGTGGCGAGAAGTTGCCAGTCGTGATCGGCGCAGGTTTCGGCGACGAGCTCCCGCCAATTTGGCACAAATTGTTGTAAATGCGCCAGCCAGTTGCGCCAAGGCTGTTTGGCGGCAAGATCGCCGCCGGGCAGAGCGACGGCAGGTAAGCCGCCTAAATAGCGCGAGGc  <  1:519511/250‑1 (MQ=255)
                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                          
GCGAGAATGGCGAAGCCTTCAGCCAACGCGTAATGGAAGATAAAGGCTTTGTCGGCCGCGGAGGCGTTGAGGGCGAGCCAGGTTTGCCAGAAGACGGCGAGATCTAATTTATTTTCATTGTTCAACGGCATTAAGATTGGAAGGTCGATTTCGTTTAATGGCAAACGGGCGAGTGTCGACTGTTCGGCGAGCACTTCTAATTGGCAGGCGGCTTCGCCTTCCCAGCTGAGTTGTGGCGGGGTGATGCCTAAGC‑CGAACGCCACGGCGTCGAAGAAGCGTCCGGCGGAAGAAATCAACGGGCTGTTGATTTTGCGTTCAATGGCGGTGGCGAGAAGTTGCCAGTCGTGATCGGCGCAGGTTTCGGCGACGAGCTCCCGCCAATTTGGCACAAATTGTTGTAAATGCGCCAGCCAGTTGCGCCAAGGCTGTTTGGCGGCAAGATCGCCGCCGGGCAGAGCGACGGCAGGTAAGCCGCCTAAATAGCGCGAGGC  >  1/1866285‑1866775

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑