| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | 1 | 1,757,453 | 0 | C | T | 50.0% | 36.2 / 34.8 | 64 | intergenic (+115095/‑24272) | unknown/unknown | tRNA‑Leu‑CAA/tRNA‑Asp‑GTC |
| Reads supporting (aligned to +/- strand): ref base C (18/14); new base T (32/0); total (50/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.97e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TCTTTTCACAAGCAAAGACAAAATCCAAGGATTTTGAACGTTGGCTTTGCCAATGGTACGAAGTGCAAGCGCAGCGCAGTAAAAGTAGGTCGATGCCGAAACAAACCTATTTATATCGCTTCTTACCACTTTCCTGATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGGAAGTA‑GTTTTCGCCGGCAGCGTA‑GGC‑GTTA‑GGTTCATTGTGGGCGTAGCGGTATTCGGCGCCGAAGCCGAGGTCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGACGTCGAAATCGGC‑GGATTC‑GGTT‑GCCAATTTTTTGGCGGC‑TTTGAAAGCGAGA‑TAAACGGCGTTGCTTTTCGGTGCGACGGCTAAATACACAATGGCTTGCGCAATAGCGCGTTCGCCTTCGGCAGCGCCGACGCGGG > 1/1757210‑1757695 | tcttttcACAAGCAAAGACAAAATCCAAGGATTTTGAACGTTGGCTTTGCCAATGGTACGAAGTGCAAGCGCAGCGCAGTAAAAGTAGGTCGATGCCGAAACAAACCTATTTATATCGCTTCTTACCACTTTCCTGATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGGAAGGA‑GTTTTTGCCGGc > 1:687864/1‑250 (MQ=255) caAGCAAAGACAAAATCCAAGGATTTTGAACGTTGGCTTTGCCAATGGTACGAAGTGCAAGCGCAGCGCAGTAAAAGTAGGTCGATGCCGAAACAAACCTATTTATATCGCTTCTTACCACTTTCCTGATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGAGAGGA‑GGTTTTGCCGGGAGCGTA‑ggg > 1:365900/1‑250 (MQ=255) aaTCCAAGGATTTTGAACGTTGGCTTTGCCAATGGTACGAAGTGCAAGCGCAGCGCAGTAAAAGTAGGTCGATGCCGAAACAAACCTATTTATATCGCTTCTTACCACTTTCCTGATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGGAAGTA‑GTTTTCGCCGGCAGCGTA‑GGC‑GTTA‑GGTTCATtg < 1:250133/251‑1 (MQ=255) gATTTTGAACGTTGGCTTTGCCAATGGTACGAAGTGCAAGCGCAGCGCAGTAAAAGTAGGTCGATGCCGAAACAAACCTATTTATATCGCTTCTTACCACTTTCCTGATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGGGAGGA‑GTTTTTGCCGGCAGCGGA‑GGC‑GTTA‑GGGTCATTGTgggggtg > 1:153257/1‑247 (MQ=255) ggCTTTGCCAATGGTACGAAGTGCAAGCGCAGCGCAGTAAAAGTAGGTCGATGCCGAAACAAACCTATTTATATCGCTTCTTACCACTTTCCTGATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGGGAGGA‑GGTTTTGCCGGCAGGGGA‑GGC‑GGTT‑GGGTCATTTTggggggaggggggtttggcg > 1:82154/1‑234 (MQ=255) aTGGTACGAAGTGCAAGCGCAGCGCAGTAAAAGTAGGTCGATGCCGAAACAAACCTATTTATATCGCTTCTTACCACTTTCCTGATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGGAAGGA‑GTTTTTGCCGGCAGCGTA‑GGC‑GTTA‑GGGTCATTGTGGGGGTAGCGGGATTCGGCGCCGAAGCCgg > 1:272967/1‑250 (MQ=255) gTGCAAGCGCAGCGCAGTAAAAGTAGGTCGATGCCGAAACAAACCTATTTATATCGCTTCTTACCACTTTCCTGATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGGAAGTA‑GTTTTTGCCGGCCGCGTA‑GGC‑GTTA‑GGGTCATTGTGGGCGGAGCGGTATTTGGCGCCGAAGCCGAGGGGTTTCAt > 1:419810/1‑251 (MQ=255) tGCAAGCGCAGCGCAGTAAAAGTAGGTCGATGCCGAAACAAACCTATTTATATCGCTTCTTACCACTTTCCTGATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGGAAGTA‑GTTTTTGCCGGCAGCGGA‑GGC‑GTTA‑GGTTCATTGTGGGCGTAGCGGGATTCGGCGCCGGAGCCGAGGGCTTTTATg > 1:298048/1‑251 (MQ=255) gCAAGAGCAGCGCAGTAAAAGTAGGTCGATGCCGAAACAAACCTATTTATATCGCTTCTTACCACTTTCCTGATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGGAAGTA‑GTTTTCGCCGGCAGCGTA‑GGG‑GGTA‑GGGTTATTGTGGGGGGAGCGGGATTTGGCGCCGGAGCCGAGGGCTTTCATGa > 1:465293/1‑251 (MQ=255) gcgcagcgcaGTAAAAGTAGGTCGATGCCGAAACAAACCTATTTATATCGCTTCTTACCACTTTCCTGATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGGAAGGA‑GTTTTCGCCGGCAGCGTA‑GGC‑ggt > 1:799192/1‑192 (MQ=255) gcgcagTAAAAGTAGGTCGATGCCGAAACAAACCTATTTATATCGCTTCTTACCACTTTCCTGATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGGGAGTA‑GGTTTTTCCGGCCGCGGA‑GGC‑GGTT‑GGTTTATTGTGGGGGGAGCGGGATTTGGGGCCGGAGGCGAGGGCTTTTATTAAGCTGGTCg > 1:27648/1‑251 (MQ=255) cagTAAAAGTAGGTCGATGCCGAAACAAACCTATTTATATCGCTTCTTACCACTTTCCTGATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGGGAGTA‑GGTTTTGCCGGCCGCGGA‑GGC‑GGTT‑GGTTCATTGTGGGGGGGGCGGTATTCGGGGCCGAAGCCGGGGGCTTTCATGAAGCTGGTCgggg > 1:720712/1‑251 (MQ=255) cagTAAAAGTAGGTCGATGCCGAAACAAACCTATTTATATCGCTTCTTACCACTTTCCTGATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGGAAGTA‑GTTTTTGCCGGCCGCGGA‑GGC‑GGTT‑GGTTCATTGTGGGGGGAGCGGGATTCGGCGCCGGAGCCGAGGGCTTTTATGAAGCTGGTCgggg > 1:426090/1‑251 (MQ=255) gTAAAAGTAGGTCGATGCCGAAACAAACCTATTTATATCGCTTCTTACCACTTTCCTGATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGGAAGTA‑GTTTTCGCCGGCAGCGGA‑gggggtagggtt > 1:937014/1‑180 (MQ=255) gTAAAAGTAGGTCGATGCCGAAACAAACCTATTTATATCGCTTCTTACCACTTTCCTGATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGGAAGGA‑GTTTTTTCCGGCAGCGTA‑GGC‑GTTT‑GGGTTATTGTGGGGGTAGCGGGATTTGGCGCCGAAGCCGAGGGGTTTCATGGAGCTGGTCgggggg > 1:615410/1‑249 (MQ=255) gTAAAAGTAGGTCGATGCCGAAACAAACCTATTTATATCGCTTCTTACCACTTTCCTGATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGGAAGGA‑GTTTTTGCCGGGAGCGTA‑GGG‑GGTT‑GGTTTATTGGGGGGGGAGCGGGATTCGGGGGCGAAGGCGAGGGCTTTCATGAAGGTGGGCgggggg > 1:473836/1‑249 (MQ=255) aaGTAGGTCGATGCCGAAACAAACCTATTTATATCGCTTCTTACCACTTTCCTGATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGGGAGTA‑GTTTTCGCCGGCAGCGTA‑GGG‑GGTA‑GGTTCATTTTGGGCGTAGCGGGATTTGGCGCCGGAGCCGAggg > 1:917968/1‑223 (MQ=255) gTCGATGCCGAAACAAACCTATTTATATCGCTTCTTACCACTTTCCTGATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGGAAGTA‑GTTTTCGCCGGCAGCGTA‑GGC‑GTTA‑GGTTCATTGTGGGCGTAGCGGTATTCGGCGCCGAAGCCGAGGTCTTTCATGAAGCTGGTCGGGGCGTTGCGgag < 1:737073/249‑1 (MQ=255) aacCTATTTATATCGCTTCTTACCACTTTCCTGATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATCGGGTGTCTTTCAGTTGTGGCGGGGAGGAGGTTTTC‑CCGGCCGCGGA‑GGC‑GGTA‑GGGTCCTTGTGGGGGGCGGGGTATTTGGCGCCGAAGCCCAGGGCTTTTATGTAGCTGGGCCGGGGGGTGCGGAGGTGTTCTGGGGCGtgga > 1:348440/1‑248 (MQ=255) ccTATTTATATCGCTTCTTACCACTTTCCTGATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGGGAGTA‑GTTTTTGCCGGCAGCGGA‑GGC‑GGTA‑GGTTCATTGTGGGGGGAGCGGGATTCGGCGCCGAAGCCGAGGGCTTTCATGAAGCTGGGCGGGGGGGTGCGGGGGTGTTTAGGGGCGTCGaaa > 1:622299/1‑251 (MQ=255) tcttACCACTTTCCTGATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGGAAGTA‑GTTTTTGCCGGCCGCGGA‑GGC‑GGTA‑GGGTCATTGTGGGGGGAGCGGGATTCGGCGCCGAAGCCGGGGGCTTTTATTAAGGTGGGCGGGGCGGTGCGGGGGGGTTCAGGGGCGGCGAAATcggggggattgggg > 1:347140/1‑240 (MQ=255) tcttACCACTTTCCTGATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGGAAGTA‑GTTTTTGCCCGCCGCGGA‑GGC‑GGTA‑GGTTCATTTTGGGGGTAGCGGGATTCGGCGCCGAAGCCGAGGTCttttctga > 1:885159/1‑190 (MQ=255) ttCCTGATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGGGAGGA‑GGTTTTGCCGGCAGCGGA‑GGC‑GGTA‑GGTTCATTGTGGGCGTAGCgg > 1:908755/1‑154 (MQ=255) ttCCTGATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGGAAGTA‑GTTTTTGCCGGGAGCGTG‑GGC‑GTTG‑GGTTTCTTGTGGGCGCAGCGGGATTTGGGGCCGAAGCCGGGGGCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGGTGGTCAgggt > 1:844081/1‑217 (MQ=255) ttCCTGATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGGAAGTA‑GGTTTTGCCGGCCGCGGA‑GGG‑GGTA‑GGTTCATTGTGGGGGTAGCGGGATTCGGCGCCGAAGCCGGGGGCTTTTCTGAAGCTGGTCGGGGCGGTGGGGGGGTGTTCAGGGACGTCGGAATCGGC‑GGGTTT‑GGTT‑GCCCAttttt > 1:1051478/1‑251 (MQ=255) ttCCTGATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGGAAGGA‑GGTTTTGCCGGCAGCGTA‑GGC‑GTTA‑GGGTCATTTTGGGGGGAGCGGGGTTCGGGGCCGCAGACGGGGTCTTTCTTTAAACTGGTCGGGGCGGTGTGGGGATGGTCAGGGGCGTCGAAATcggc‑ggggttgggttcgcagtttt > 1:822884/1‑233 (MQ=255) ttCCTGATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGGAAGGA‑GGTTTCGCCGGCAGCGGA‑GGC‑GGTAGGGTTC‑TTTTGGGGGGAGGGGTATTTGGCGCCGAAGCCGAGGGCTTTTATGAAGCTCGTCGGGGCGGTGGGGGGATGGTCAGGGACGGCGGAATCGGC‑GGGGTC‑GGTT‑GGCAAttttt > 1:358984/1‑251 (MQ=255) ttCCTGATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGGAAGGA‑GGTTTCGCCCGGAGCGGA‑GGG‑GGGA‑GGGTTATTGTGGGGGGAGCGGGATTCGGGGCCGGAGGCGAGGGCTTTCATGAAGGTGGGCGGGGCGCTGCGGAGATGGTTAGGGGCGTCGAAATCGGC‑GGAGTA‑GGTT‑ggccattatt > 1:604370/1‑242 (MQ=255) gATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGGGAGGA‑GTTTTCGCCCGCAGCGTA‑GGC‑GGTA‑GGGTCCTTGTGGGCGGCGGGGGGTTCGGCGCCGGAGCCGAGGGCTTTCATGAAGCTGGTCGGGGGGGTGCGGGGA‑GGTCAGGGGCGGCGAAATCGGC‑GGGTTC‑GGGT‑GCCCATTTTTTtgcgg > 1:847583/1‑251 (MQ=255) gATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGGAAGTA‑GTTTTTGCCGGCAGCGTA‑GGC‑GTTA‑GGGTCATTGTGGGCGGAGCGGGATTCGGCGCCGGAGCCGAGGGCTTTCATGAAGCTGGGCGGGGCGGTGCGGGGAGGTTCAGGGGCGTCGAAATCGGC‑GGGTTC‑GGGT‑GCCCAtttttttggg > 1:122573/1‑247 (MQ=255) gATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGGAAGTA‑GTTTTCGc < 1:10326/111‑1 (MQ=255) gATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGGAAGTA‑GGTTTTGCCGGCAGCGGA‑GGC‑GTTA‑GGGTCATTGTGGGCGGAGCGGGATTCGgc > 1:756709/1‑157 (MQ=255) aaCCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGGAAGTA‑GTTTTTTCCGGCAGCGTA‑GGC‑GTTA‑GGGTTATTGTGGGGGGAGCGGGATTc > 1:304661/1‑149 (MQ=255) ccGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGGAAGTA‑GTTTTCGCCGGCAGCGTA‑GGC‑GTTA‑GGTTCATTGTGGGCGTAGCGGTATTCGGCGCCGAAGCCGAGGTCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGACGTCGAAATCGGC‑GGATTC‑GGTT‑GCCAATTTTTTGGCGGC‑TTTGAAAg < 1:268184/251‑1 (MQ=255) aTTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGGAAGTA‑GTTTTCGCCGGCAGCGTA‑GGC‑GGTT‑GGGTCCTTTTGGGGGGAGCGGGATTTGGCGCCGAAGCCGAGGGCTTTTATGAAGCTGGTCGGGGGGTTGCGGGGATGTTCAGGGAGGTCGAAATCGGC‑GGGGTC‑GGTT‑TGCAATTTTTTtgcgggcttgtaagggaggtaa > 1:902754/1‑234 (MQ=255) ttCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGGAAGGA‑GTTTTTGCCGGCCGCGGA‑GGC‑GTTT‑GGGTCATTGTGGGCGTAGCGGGATTCGGCGCCGAAGCCGAGGGCTTTCATGAAGCTGGTCGGGGGGTTGCGGAGATGTTCAGGGGCGTCGAAATCGGCGGGATT‑‑GGGT‑GCCCAttttttttgggccttttaagcgagataaa > 1:1032010/1‑228 (MQ=255) tCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGGAAGTA‑GTTTTCGCCGGCAGCGTA‑GGC‑GTTA‑GGTTCATTGTGGGCGTAGCGGTATTCGGCGCCGAAGCCGAGGTCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGACGTCGAAATCGGC‑GGATTC‑GGTT‑GCCAATTTTTTGGCGGC‑TTTGAAAGCGAGA‑TAAAc < 1:1044037/251‑1 (MQ=255) ccTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGGAAGTA‑GTTTTCGCCCGGAGCGTA‑GGC‑GTTA‑GGTTCATTGTGGGCGTAGCGGGATTCGGCGCCGAAGCCGAGGGCTTTCATGAAGCTGGTCGGGGGGGTGTGGGGATGTTTCGGGGCGTCGGAAACGGC‑GGGTTT‑AGTT‑GGCAATTTTTTTGGGGG‑TTTGAAAACGAGA‑AAAACGGCGTTGCtt > 1:1045445/1‑251 (MQ=255) atcttaatTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGGAAGGA‑GGTTTTGCCGGCAGCGGA‑GGC‑GGTA‑GGTTCATTGTGGGCGGAGCGGGATTCGGTGCCGGAGCCGAGGGCTTTCATGAAGCTGGGCGGGGCGGTGCGGAGATGGTCAGGGACGGCGAAAACGGCGGGGTTG‑GGTG‑GCCAATTTTTTGGGGGT‑TTTGaaaagagaaaaaaggcgggggtttttcg > 1:921821/1‑226 (MQ=255) cttaatTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGGGAGTA‑GTTTTCGCCGGCAGCGTA‑GGC‑GGTA‑GGTTCATTGTGGGCGGAGCGGGATTCGGCGCCGAAGCCGAGGGCTTTTATGAAGCTGGGCGGGGCGGTGCGGGGATGTTCAGGGACGTCGAAATCGGC‑GGGGTT‑GGTT‑GCCCATTTTTTTTCGGG‑CTTGTAAACGAGA‑TAAAAAGCGTGGCTTTTTGGTg > 1:121376/1‑251 (MQ=255) ccATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGGGAGGA‑GTTTTTGCCGGGAGCGTA‑GGG‑GGTA‑GGTTCATTGTGGGCGTAGGGGTATTCGGCGCCGAAGCCGAGGGCTTTTATGAAGCTGGTCGGGGCGTTGCGGAGATGTTTAGGGACGTCGAAAACGGC‑GGGGTC‑GGTTGGCCATTTTTTTGGGGGC‑TTTG‑AAGCGGGA‑TAAAAGGCGGTGCTTTTTGGTGGGACGGc > 1:294287/1‑250 (MQ=255) tGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGGAAGTA‑GTTTTCGCCGGCAGCGTA‑GGC‑GTTA‑GGTTCATTGTGGGCGTAGCGGTATTCGGCGCCGAAGCCGAGGTCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGACGTCGAAATCGGC‑GGATTC‑GGTT‑GCCAATTTTTTGGCGGC‑TTTGAAAg < 1:862941/213‑1 (MQ=255) tGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGGAAGTA‑GTTTTCGCCGGCAGCGTA‑GGC‑GTTA‑GGTTCATTGTGGGCGTAGCGGTATTCGGCGCCGAAGCCGAGGTCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGACGTCGAAATCGGC‑GGATTC‑GGTT‑GCCAATTTTTTGGCGGC‑TTTGAAAGCGAGA‑TAAACGGCGTTGCTTTTCGGTGCGACGGCTaaa < 1:732644/251‑1 (MQ=255) ccTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGGGAGTA‑GTTTTTGCCGGCAGCGGA‑GGC‑GGTT‑GGGTCATTTTGGGCGTAGCGGGATTTGGCGCCGGAGCCGAGGGCTTTTATGAAGCTGGTCGGGGGGTTGCGGGGATGTTCAgggg > 1:7192/1‑162 (MQ=255) ccTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGGGAGTA‑GGTTTTGCCGGCAGCGGAGGGG‑GTTG‑GGTT‑ATTTTGGGGGTAGCGGGATTCGGCGCCGAAGCCGAGGGCTTTCATGAAGCTGGGCGGGGGGGTGCGGAGGTGTTTCGGGGCGTCGAAATCGGCGGGGTTG‑GGTG‑GCCAATTTTTTGGGGGT‑TTTTAAAG‑GAGG‑TAAAAGGCGTTGGtttttgggtgtctcttttaca > 1:250303/1‑233 (MQ=255) ccTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGGGAGGA‑GGTTTTGCCGGGAGCGTA‑GGG‑GGTA‑GGTTCATTTTGGGCGTAGGGGGATTTGGGGGCGGAGCCGAGGGGTTTTCTGAAGCTGGGCGGGGGGGTGCGGGGAAGGTTAGGGGCGGCGAAATCGGG‑GGGTTT‑GGTT‑GGCAATTTTTTTTGGGG‑TTTGTAAGAGAGA‑AAAAAAGCGGTGCTTTTTGGTGGGACGGGTAAATa > 1:824100/1‑251 (MQ=255) ccTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGGAAGGA‑GTTTTTGCCGGCAGCGTA‑GGC‑GTTT‑GGTTCATTGTGGGGGGAGCGGGATTCGGCGCCGAAGCCGAGGGCTTTCTTGAAGCTGGTCGGGGCGGTGCGGGGATGTTCAGGGACGTCGAAAACGGC‑GGGGTC‑GGGT‑GCCCATTTTTTTGGGGCTTTTGAAA‑CGAGA‑TAAAAAGCGGTGGTTTTTGGTGCGGCGGGTAAATa > 1:712888/1‑251 (MQ=255) ccTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGGAAGGA‑GTTTTCGCCCGCAGCGTA‑GGC‑GGTA‑GGGTTATTGTGGGCGGAGCGGTATTTGGGGCCGAAGGCGAGGGCTTTCATGAAGCTGGTCGGGGCGTTGCGGGGGTGTTCAGGGa > 1:714536/1‑163 (MQ=255) ccTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGGAAGGA‑GGTTTTGCCGGCAGCGGA‑GGCGGTTG‑GGTT‑ATTGTGGGCGGAGCGGGATTCGGCGCCGAAGCCGAGGGCTTTTATGAAGCTGGGCGGGGCGGTGCGGAGATGGTCAGGGAAGGCGGAATCGGC‑GGGTTC‑gg > 1:789061/1‑184 (MQ=255) tggtggTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGGAAGTA‑GTTTTCGCCGGCAGCGTA‑GGC‑GTTA‑GGTTCATTGTGGGCGTAGCGGTATTCGGCGCCGAAGCCGAGGTCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGACGTCGAAATCGGC‑GGATTC‑GGTT‑GCCAATTTTTTGGCGGC‑TTTGAAAGCGAGA‑TAAACGGCGTTGCTTTTCGGTGCGACGGCTAAATAcaca < 1:928825/251‑1 (MQ=255) tCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGGAAGTA‑GTTTTCGCCGGCAGCGTA‑GGC‑GTTA‑GGTTCATTGTGGGCGTAGCGGTATTCGGCGCCGAAGCCGAGGTCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGACGTCGAAATCGGC‑GGATTC‑GGTT‑GCCAATTTTTTGGCGGC‑TTTGAAAGCGAGA‑TAAACGGCGTTGCTTTTCGGTGCGACGGCTAAATACACAATGGCt < 1:11021/251‑1 (MQ=255) aaaTAGAATTGGGTGTCTTTCAGTTGTGGCGGGAAGTA‑GTTTTTGCCGGCCGCGGA‑GGG‑GTTA‑GGGTCATTGTGGGGGTAGCGGGATTCGGCGCCGAAGCCGAGGGCTTTTATGAAGCTGGTCGGGGGGTTGCGGGGATGTTCAGGGACGGCGAAATCGGC‑GGGGTCGGGTG‑GCCATTTTTTTGG‑GGG‑TTTTTAAGAGAGA‑TAAACGGGGGTGCTTTTTGGGGCGACGGCTTAATAAACAATGGCTTgcgg > 1:646577/1‑250 (MQ=255) aaaTAGAATTGGGTGTCTTTCAGTTGTGGCGGGAAGTA‑GTTTTCGCCGGCCGCGTA‑GGC‑GTTT‑GGGTCATTGTGGGCGTAGCGGGATTCGGCGCCGAAGCCGAGGGCTTTCATGAAGCTGGTCGGGGCGTTGCGGGGATGTTCAGGGACGGCGAAATCGGC‑GGGTTC‑GGGT‑GGCCATTTTTTTGCGGCGTTTTAAA‑CGAGA‑TAAAAGGCGGTGCTTTTCTGTGCGGCGGCTAAATACACAAAAGCTTgcg > 1:416370/1‑250 (MQ=255) aTAGAATTGGGTGTCTTTCAGTTGTGGCGGGGAGGA‑GTTTTTGCCGGCCGCGGA‑GGC‑GGTT‑GGGTCATTTTGGGGGTAGCGGGATTTGGCGCCGAAGCCGAGGGCTTTCCTGAAGCTGGTCGGGGGGGTGCGGAGATGTTTAGGGGCGTCGGAATCGGC‑GGGTTT‑GGGT‑GCCCATTTTTTTGGGGC‑TTTGAAAAAGAGA‑GAAAAGGCGGTGCTTTTTGGTGCGACGGCTAAATAAACAAAAGCTTgcggca > 1:1019869/1‑248 (MQ=255) tAGAATTGGGTGTCTTTCAGTTGTGGCGGGAAGTA‑GTTTTCGCCGGCAGCGTA‑GGC‑GTTA‑GGTTCATTGTGGGCGTAGCGGTATTCGGCGCCGAAGCCGAGGTCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGACGTCGAAATCGGC‑GGATTC‑GGTT‑GCCAATTTTTTGGCGGC‑TTTGAAAGCGAGA‑TAAACGGCGTTGCTTTTCGGTGCGACGGCTAAATACACAATGGCTTGCGCAAt < 1:895484/251‑1 (MQ=255) gAATTGGGTGTCTTTCAGTTGTGGCGGGGAGTA‑GTTTTCGCCGGCCGCGGA‑GGCGGTTG‑GGTT‑ATTGTGGGGGGAGCGGGTTTCGGCGCCGAAGCCCAGGGCTTTCATGAAGCTCGTCGGGGGGTTGCGGGGATGGTCAGGGGCGTCGGAATCGGC‑GGGTTT‑GGTT‑GCCCATTTTTTTGGGGC‑CTTGAAAACGGGATTAAAAGGGGTGGC‑TTTCCGTGGGACGGCTAAATAAACaaatggtttcggaaaaa > 1:676978/1‑236 (MQ=255) gAATTGGGTGTCTTTCAGTTGTGGCGGGAAGGA‑GTTTTCGCCGGCAGCGTA‑GGC‑GGTA‑GGTTTATtg > 1:770505/1‑67 (MQ=255) aaTTGGGTGTCTTTCAGTTGTGGCGGGAAGTA‑GTTTTCGCCGGCAGCGTA‑GGC‑GTTA‑GGTTCATTGTGGGCGTAGCGGTATTCGGCGCCGAAGCCGAGGTCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGACGTCGAAATCGGC‑GGATTC‑GGTT‑GCCAATTTTTTGGCGGC‑TTTGAAAGCGAGA‑TAAACGGCGTTGCTTTTCGGTGCGACGGCTAAATACACAATGGCTTGCGCAATAg < 1:103329/250‑1 (MQ=255) aaTTGGGTGTCTTTCAGTTGTGGCGGGAAGTA‑GTTTTCGCCGGCAGCGTA‑GGC‑GTTA‑GGTTCATTGTGGGCGTAGCGGTATTCGGCGCCGAAGCCGAGGTCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGACGTCGAAATCGGC‑GGATTC‑GGTT‑GCCAATTTTTTGGCGGC‑TTTGAAAGCGAGA‑TAAACGGCGTTGCTTTTCGGTGCGACGGCTAAATACACAATGGCTTGCGCAATAg < 1:586185/250‑1 (MQ=255) ttGGGTGTCTTTCAGTTGTGGCGGGAAGTA‑GTTTTCGCCGGCAGCGTA‑GGC‑GTTA‑GGTTCATTGTGGGCGTAGCGGTATTCGGCGCCGAAGCCGAGGTCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGACGTCGAAATCGGC‑GGATTC‑GGTT‑GCCAATTTTTTGGCGGC‑TTTGAAAGCGAGA‑TAAACGGCGTTGCTTTTCGGTGCGACGGCTAAATACACAATGGCTTGCGCAATAgcgc < 1:790026/251‑1 (MQ=255) tttCAGTTGTGGCGGGAAGTA‑GTTTTCGCCGGTAGCGTA‑GGC‑GTTA‑GGTTCATTGTGGGCGTAGCGGTATTCGGCGTCGAAGCCGAGGTCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGACTTCGAAATCGGC‑GGATTC‑GGTT‑GCCAATTTTTTGGCGGC‑TTTGAAAGCGAGA‑TAAACGGCGTTGCTTTTCGGTGCGACGGCTAAATACACAATgg < 1:468781/227‑1 (MQ=255) ggAAGTA‑GTTTTCGCCGGCAGCGTA‑GGC‑GTTA‑GGTTCATTGTGGGCGTAGCGGTATTCGGCGCCGAAGCCGAGGTCTTTCATGAAGCTGGTCGGGGCGTTGCGgag > 1:507558/1‑106 (MQ=255) gta‑gtTTTCGCCGGCAGCGTA‑GGC‑GTTA‑GGTTCATTGTGGGCGTAGCGGTATTCGGCGCCGAAGCCGAGGTCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGACGTCGAAATCGGC‑GGATTC‑GGTT‑GCCAATTTTTTGGCGGC‑TTTGAAAGCGAGA‑TAAAc > 1:607054/1‑171 (MQ=255) gta‑gtTTTCGCCGGCAGCGTA‑GGC‑GTTA‑GGTTCATTGTGGGCGTAGCGGTATTCGGCGCCGAAGCCGAGGTCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGACGTCGAAATCGGC‑GGATTC‑GGTT‑GCCAATTTTTTGGCGGC‑TTTGAAAGCGAGA‑TAAACGGCGTTGCTTTTCGGTGCGACGGCTAAATACACAATGGCTTGCGCAATAGCGCGTTCGCCTTCGGCAGCGCCGACGCggg > 1:216897/1‑251 (MQ=255) | TCTTTTCACAAGCAAAGACAAAATCCAAGGATTTTGAACGTTGGCTTTGCCAATGGTACGAAGTGCAAGCGCAGCGCAGTAAAAGTAGGTCGATGCCGAAACAAACCTATTTATATCGCTTCTTACCACTTTCCTGATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGGAAGTA‑GTTTTCGCCGGCAGCGTA‑GGC‑GTTA‑GGTTCATTGTGGGCGTAGCGGTATTCGGCGCCGAAGCCGAGGTCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGACGTCGAAATCGGC‑GGATTC‑GGTT‑GCCAATTTTTTGGCGGC‑TTTGAAAGCGAGA‑TAAACGGCGTTGCTTTTCGGTGCGACGGCTAAATACACAATGGCTTGCGCAATAGCGCGTTCGCCTTCGGCAGCGCCGACGCGGG > 1/1757210‑1757695 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |