Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,405,369	0	C	T	40.0%	79.7 / 23.1	55	intergenic (‑11982/+7936)	unknown/unknown	tRNA‑Val‑GAC/LSU rRNA ## 23S rRNA, large subunit ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base C (29/4);  new base T (0/22);  total (29/26)
Fisher's exact test for biased strand distribution p-value = 1.15e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

ATGGACTATCAATCTGCCGGTGCGGGCAAACTCATGGCAAAAATTGCCACGGAAAAAGAATCCGGTCAGATTATGGCAGATGTTATTTGGA‑CCAGTGAAGTCCCTGACTTCTTCAATATGAAAGAAACCGGCATGCTAGAAACTTATATTTCGCCCGAAGTAGAAAATATCATTAATCCGATTCCCGATTTCGACGGTTCTTTTA‑CGCCGATACGCTTAGGCACATTAGGTATCGCCTACAACACCCGCTTTGTGAAAAAAGAACCGCCGGCACAATGGGCGGATATTCTTACTCCGCAATTTAAAGGCGCATTCGGTATTGCCAACCCTGCCCTAAACATTTACAATAATCCGGTGAATAAATTCGTGTTTAATTTCATTGGGCTATCCAATCAGTTGAACGTCAATTTGTCTGCGCAAGGTGTCACTATTGATCATTGTGAAGGTGTTTTTAATATTCAACCGACGCCTCCGGCAGAAT  >  1/1405121‑1405601                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                         aTGGACTATCAATCTGCCGGTGCGGGCAAACTCATGGCAAAAATTGCCACGGAAAAAGAATCCGGTCAGATTATGGCAGATGTTATTTGGA‑CCAGTGAAGTCCCTGACTTCTTCAATATGAAAGAAACCGGCATGCTAGAAACTTATATTTCGCCCGAAGTAGAAAATATCATTAATCCGATTCCCGATTTCGACGGTTCTTTTA‑CGCCGATACGCTTAGGCACATTAGGTATCGCCTACAACACCCGCtt                                                                                                                                                                                                                                        <  1:854597/251‑1 (MQ=255)                                     gaaaaaatgcccACGGAAAAAGAACCCGGTCAGATTATGGCAGTTTTTTTTTGGC‑CCAGTGAATTCCCTGATTTTTTAAATATAAAAGAACCCGGCATGCTAGAATCTTATATTCCGCCCGAAGAAAAAAATATCATTAACCCGATCCCCGTTTTCGACGTTTCTTTTC‑CGCCAATCCGCTTAGGCACATTAGGTATCGCCTACAACCCCCGTTTTGTAAAAAAAGAACCGCCGGCACAATGGGCGGata                                                                                                                                                                                                     <  1:101095/240‑1 (MQ=255)                                      cAAAAATTGCCACGGAAAAAGAATCCGGTCAGATTATGGCAGATGTTATTTGGA‑CCAGTGAAGTCCCTGACTTCTTCAATATGAAAGAAACCGGCATGCTAGAAACTTATATTTCGCCCGAAGTAGAAAATATCATTAATCCGATTCCCGATTTCGACGGTTCTTTTA‑CGCCGATACGCTTAGGCACATTAGGTATCGCCTACAACACCCGCTTTGTGAAAAAAGAGCCGCCGGCACAATGGGCGGatat                                                                                                                                                                                                    <  1:221338/250‑1 (MQ=255)                                       aaaaaTTGCCACGGAAAAAGAATCCGGTCAGATTATGGCAGATGTTATTTGGA‑CCAGTGAAGTCCCTGCCTTCTTCAATATAAAAGAACCCGGCATGCAAGAAACTTATATTCCCCCCGAAGTAAAAAATATCATTAACCCGATCCCCGTTTTCGGCGTTTTTTTTC‑CGCCGATCCGCTTAGGCACATTAGGTACCGCCTACAACCCCCGTTTTGTAAAAAAAGAACCGCCGGCACAATGGGCGGATATTc                                                                                                                                                                                                  <  1:133018/251‑1 (MQ=255)                                       aaaaaTTGCCACGAAAAAAAAACCGGGTCAGATTTGGGCAGTTGTTATTTGGA‑CCGGTAAAGTCCCTGATTTCTTAAATAAAAAAAAACCCGGCTTGCTAAAAATTTATTTTCCGCCCGAAGTAAAAAATATCATTAACCCGATCCCCGTTTTCGCCGGTTTTTTTA‑CGCCGATCCGTTTAGCCACATTAGGTATCGCCTAAAACCCCCGTTTTGTAAAAAAAAAACCGCCGGCACAATGGGCGGATATTc                                                                                                                                                                                                  <  1:294375/251‑1 (MQ=255)                                                    gAAAAAGAATCCGGTCAGATTATGGCAGATGTTATTTGGA‑CCAGTGAAGTCCCTGACTTCTTCAATATGAAAGAAACCGGCATGCTAGAAACTTATATTTCGCCCGAAGTAGAAAATATCATTAATCCGATTCCCGATTTCGACGGTTCTTTTA‑CGCCGATACGCTTAGGCACATTAGGTATCGCCTACAACACCCGCTTTGTGAAAAAAGAACCGCCGGCACAATGGGCGGATATTCTTACTCCGCAAtt                                                                                                                                                                                     >  1:515536/1‑251 (MQ=255)                                                     aaaaaaaaccgggTCAGATTATGGAAGATTTTATTTGGA‑CCAGTAAAGTCCCTGCCTTCTTCAATATAAAAGAACCCGGCATGCTAAAATTTTATTTTCCGCCCGAAGAAAAAAATATCATAAACCCGTTCCCCGATTTCGACGGTTTTTTTA‑CGCGGAGACGCTTAGGCAATTTAGGTTTCCCTTACACCCCCCGTTTTGTAAAAAAAAAACCGCCGGCACAATGGGCGGATATTCTTACTCCGCAAttt                                                                                                                                                                                    <  1:804294/240‑1 (MQ=255)                                                                ggTCAGATTATGGCAGATGTTATTTGGA‑CCAGTGAAGTCCCTGACTTCTTCAATATGAAAGAAACCGGCATGCTAGAAACTTATATTTCGCCCGAAGTAGAAAATATCATTAATCCGATTCCCGATTTCGACGGTTCTTTTA‑CGCCGATACGCTTAGGCACATTAGGTATCGCCTACAACACCCGCTTTGTGAAAAAAGAACCGCCGGCACAATGGGCGGATATTCTTACTCCGCAAttt                                                                                                                                                                                    >  1:144638/1‑240 (MQ=255)                                                                     gATTATGGCAGATGTTATTTGGA‑CCAGTGAAGTCCCTGACTTCTTCAATATGAAAGAAACCGGCATGCTAGAAACTTATATTTCGCCCGAAGTAGAAAATATCATTAATCCGATTCCCGATTTCGACGGTTCTTTTA‑CGCCGATACGCTTAGGCACATTAGGTATCGCCTACAACACCCGCTTTGTGAAAAAAGAACCGCCGGCACAATGGGCGGATATTCTTACTCCGCAATTTAAAGGCGCATTCGGTa                                                                                                                                                                    >  1:146997/1‑251 (MQ=255)                                                                     gATTATGGCAGATGTTATTTGGA‑CCAGTGAAGTCCCTGACTTCTTCAATATGAAAGAAACCGGCATGCTAGAAACTTATATTTCGCCCGAAGTAGAAAATATCATTAATCCGATTCCCGATTTCGACGGTTCTTTTA‑CGCCGATACGCTTAGGCACATTAGGTATCGCCTACAACACCCGCTTTGTGAAAAAAGAACCGCCGGCACAATGGGCGGATATTCTTACTCCGCAATTTAAAGGCGCATTCGGTa                                                                                                                                                                    >  1:216074/1‑251 (MQ=255)                                                                     gATTATGGCAGATGTTATTTGGA‑CCAGTGAAGTCCCTGACTTCTTCAATATGAAAGAAACCGGCATGCTAGAAACTTATATTTCGCCCGAAGTAGAAAATATCATTAATCCGATTCCCGATTTCGACGGTTCTTTTA‑CGCCGATACGCTTAGGCACATTAGGTATCGCCTACAACACCCGCTTTGTGAAAAAAGAACCGCCGGCACAATGGGCGGATATTCTTACTCCGCAATTTAAAGGCGCATTCGGTa                                                                                                                                                                    >  1:871829/1‑251 (MQ=255)                                                                     gATTATGGCAGATGTTATTTGGA‑CCAGGGAAGTCCCTGACTTCTTCAATATGAAAGAAACCGGCATGCTAGAAACTTATATTTCGCCCGAAGTAGAAAATATCATTAATCCGATTCCCGATTTCGACGGTTCTTTTA‑CGCCGATACGCTTAGGCACATTAGGTATCGCCTACAACACCCGCTTTGTGAAAAAAGAACCGCCGGCACAATGGGCGGATATTCTTACTCCGCAATTTAAAGGCGCATTCGGTa                                                                                                                                                                    >  1:622549/1‑251 (MQ=255)                                                                          tGGCAGATGTTATTTGGA‑CCAGTGAAGTCCCTGACTTCTTCAATATGAAAGAAACCGGCATGCTAGAAACTTATATTTCGCCCGAAGTAGAAAATATCATTAATCCGATTCCCGATTTCGACGGTTCTTTTA‑CGCCGATACGCTTAGGCACATTAGGTATCGCCTACAACACCCGCTTTGTGAAAAAAGAACCGCCGGCACAATGGGCGGATATTCTTACTCCGCAATTTAAAGGCGCATTCGGTATTGc                                                                                                                                                                >  1:687789/1‑250 (MQ=255)                                                                               taTGTTATTGGGA‑CCAGTGAAGTCCCTGACTTCTTCAATATAAAAGAACCCGGCATGCTAGAAACTTATATTCCGCCGGAAGTAAAAAATATCATTAACCCGATCCCCGATTCCGCCGGTTTTTTTC‑CGCCGATACGCTTGGCCACATTAGGTATCGCCAACACCCCCCGTTTTGTAAAAAAAGAACCGCCGGCACAATGGGCGGATATTCTTACTCCGCAATTTAAAGGCGCATTCGGTATTGCCAAccc                                                                                                                                                          <  1:737819/250‑1 (MQ=255)                                                                                aTGTTATTTGGA‑CCAGTGAAGTCCCTGACTTCTTCAATATGAAAGAAACCGGCATGCTAGAAACTTATATTTCGCCCGAAGTAGAAAATATCATTAATCCGATTCCCGATTTCGACGGTTCTTTTA‑CGCCGATACGCTTAGGCACATTAGGTATCGCCTACAACACCCGCTTTGTGAAAAAAGAACCGCCGGCACAATGGGCGGATATTCTTACTCCGCAATTTAAAGGCGCATTCGGTATTGCCAAcc                                                                                                                                                           >  1:345033/1‑249 (MQ=255)                                                                                aTGTTATTTGGA‑CCAGTGAAGCCCCTGACTTTTTAAATATGAAAGAAACCGGCATGCTAGAAACTTATATTCCGCCCGAAGTAAAAAATATCATTAACCCGATCCCCGATTTCGCCGGTTCTTTTA‑CGCCAATCCGCTTAGGCACATTAGGTACCGCCTACACCCCCCGTTTTGTAAAAAAAGAACCGCCGGCACAATGGGCGGATATTCTTACTCCGCAATTTAAAGGCGCATTCGGTATTGCCAAccc                                                                                                                                                          <  1:245044/250‑1 (MQ=255)                                                                                aTGTTA‑TTGGACCCAGTGAAGCCCCTGCCTTCTTCAATATAAAAAAACCCGGCATGCTAGAAATTTATTTTTCGCCCGAAGTAAAAAATATCATTAATCCGATCCCCGATTTCGGCGGTTTTTTTC‑CGCCGATACGTTTAGCCACATTAGGTACCGCCTACAACCCCCGTTTTGTAAAAAAAGAACCGCCGGCACAATGGGCGGATATTCTTACTCCGCAATTTAAAGGCGCATTCGGTATTGCCAAccc                                                                                                                                                          <  1:222280/250‑1 (MQ=255)                                                                                     aTTTGGA‑CCAGTGAAGTCCCTGACTTCTTCAATATGAAAGAAACCGGCATGCTAGAAACTTATATTTCGCCCGAAGTAGAAAATATCATTAATCCGATTCCCGATTTCGACGGTTCTTTTA‑CGCCGATACGCTTAGGCACATTAGGTATCGCCTACAACACCCGCTTTGTGAAAAAAGAACCGCCGGCACAATGGGCGGATATTCTTACTCCGCAATTTAAAGGCGCATTCGGTATTGCCAAccc                                                                                                                                                          >  1:898528/1‑245 (MQ=255)                                                                                                gTGAAGTCCCTGACTTCTTCAATATGAAAGAAACCGGCATGCTAGAAACTTATATTTCGCCCGAAGTAGAAAATATCATTAATCCGATTCCCGATTTCGACGGTTCTTTTA‑CGCCGATACGCTTAGGCACATTAGGTATCGCCTACAACACCCGCTTTGTGAAAAAAGAACCGCCGGCACAATGGGCGGATATTCTTACTCCGCAATTTAAAGGCGCATTCGGTATTGCCAACCCTGccc                                                                                                                                                     >  1:729261/1‑240 (MQ=255)                                                                                                gTGAAGTCCCTGACTTCTTCAATATGAAAGAAACCGGCATGCTAGAAACTTATATTTCGCCCGAAGTAGAAAATATCATTAATCCGATTCCCGATTTCGACGGTTCTTTTA‑CGCCGATACGCTTAGGCACATTAGGTATCGCCTACAACACCCGCTTTGTGAAAAAAGAACCGCCGGCACAATGGGCGGATATTCTTACTCCGCAATTTAAAGGCGCATTCGGTATTGCCAACCCTGCCCTAAACATTTa                                                                                                                                           >  1:86255/1‑250 (MQ=255)                                                                                                  aaaGTCCCTGACTTTTTAAATAAAAAAGAACCCGGCTTGCAAAAAACTTATATTTCGCCCGAAGAAAAAAATATCATTAATCCGATCCCCGATTTCGACGGTTTTTTTC‑CGCCGAACCGCTAGGGCACATAAGGTACCGCCAACACCCCCCGCTTTGTAAAAAAAGAACCGCCGGCACAATGGGCGGATATTCTTACTCCGCAATTTAAAGGCGCATTCGGTATTGCCAACCCTGCCCTAAACATTTACaa                                                                                                                                        <  1:762741/250‑1 (MQ=255)                                                                                                    aGTCCCTGTCTTCTTAAATATGAAAGAACCCGGCATGATAGAAATTTATTTTTCGCCCGAAGTAAAAAATATCATTAATCCGATCCCCGTTTTCGACGGTTTTTTTC‑CGCCGATACGCTTAGGCACATTAGGTATGGCCTACAACCCCCGTTTTGTAAAAAAAGAACCGCCGGCACAATGGGCGGATATTCTTACTCCGCAATTTAAAGGCGCATTCGGTATTGCCAACCCTGccc                                                                                                                                                     <  1:685829/236‑1 (MQ=255)                                                                                                     gTCCCTGACTTCTTCAATATGAAAGAAACCGGCATGCTAGAAACTTATATTTCGCCCGAAGTAGAAAATATCATTAATCCGATTCCCGATTTCGACGGTTCTTTTA‑CGCCGATACGCTTAGGCACATTAGGTATCGCCTACAACACCCGCTTTGTGAAAAAAGAACCGCCGGCACAATGGGCGGATATTCTTAc                                                                                                                                                                                              >  1:998960/1‑194 (MQ=255)                                                                                                                    aaTATAAAAGAACCCGGCATGCTAAAATCTTATTTTCCCCCCGAAGAAAAAAATATCATTATCCCGTTCCCCTATTTGGCCGTTTTTTTTC‑CGCCAATCCGTTTAGGCACATTAGGTTCCCCCTCCAACCCCCGTTTTGTAAAAAAAAAACCGCCGGCACAATGGGCGGATATTCTTACTCCGCAATTTAAAGGCGCATTCGGTATTGCCAACCCTGCCCTAAACATTTACAATAATCCGGTGAATAAAtt                                                                                                                      <  1:528036/251‑1 (MQ=255)                                                                                                                     atatGAAAGAAACCGGCATGCTAGAAACTTATATTTCGCCCGAAGTAGAAAATATCATTAATCCGATTCCCGATTTCGACGGTTCTTTTA‑CGCCGATACGCTTAGGCACATTAGGTATCGCCTACAACACCCGCTTTGTGAAAAAAGAACCGCCGGCACAATGGGCGGATATTCTTACTCCGCAATTTAAAGGCGCATTCGGTATTGCCAACCCTGCCCTAAAc                                                                                                                                                >  1:876856/1‑224 (MQ=255)                                                                                                                                        cctaaaaaCTTATTTTCCCCCCAAAGTAAAAAATATCATTAATCCGATCCCCTATTTCGACGGTTTTTTTC‑CGCCGATACGCTAAGCCACATTGGGTACCGCCTACACCACCCGTTTTGTAAAAAAAGAACCGCCGGCACAATGGGCGGATATTCTTACTCCGCAATTTAAAGGCGCATTCGGTATTGCCAACCCTGCCCTAAACATTTACAATAATCCGGTGAATAAATTCGTGTTTAATTTCATTGGGc                                                                                                  <  1:887243/246‑1 (MQ=255)                                                                                                                                         cTAGAAGCTTATATTCCCCCAGAAGTAGAAAATATCATTAATCCAGTTCCCTATTTGGGCGGTTTTTTGA‑CGCGGATGCGATTAGGCAAATTAGTTATCGCCTACAACACCCGTTTTGTAAAAAAAGAACCGCCGGCACAATGGGCGGATATTCTTACTCCGCAATTTAAAGGCGCATTCGGTATTGCCAACCCTGCCCTAAACATTTACAATAATCCGGTGAATAAATTCGTGTTTAATTTCATTGGGc                                                                                                  <  1:705604/250‑1 (MQ=255)                                                                                                                                              aaCTTATATTTCGCCCGAAGTAGAAAATATCATTAATCCGATTCCCGATTTCGACGGTTCTTTTA‑CGCCGATACGCTTAGGCACATTAGGTATCGCCTACAACACCCGCTTTGTGAAAAAAGAACCGCCGGCACAATGGGCGGATATTCTTACTCCGCAATTTAAAGGCGCATTCGGTATTGCCAACCCTGCCCTAAACATTTACAATAATCCGGTGAATAAATTCGTGTTTAATTTCATTGGGCTATCCa                                                                                            >  1:414369/1‑251 (MQ=255)                                                                                                                                                tttattttccccccGAAGTAAAAAATATCATTAACCCGTTCCCCGATTTCGAGGTTTTTTTTA‑CGCCGATACGCTTAGGCACATTAGGTATCGCCTACACCCCCCGTTTTGTAAAAAAAGAACCGCCGGCACAATGGGCGGata                                                                                                                                                                                                     <  1:992580/133‑1 (MQ=255)                                                                                                                                                                 gTAGAAAATATCATTAATCCGATTCCCGATTTCGACGGTTCTTTTA‑CGCCGATACGCTTAGGCACATTAGGTATCGCCTACAACACCCGCTTTGTGAAAAAAGAACCGCCGGCACAATGGGCGGATATTCTTACTCCGCAATTTAAAGGCGCATTCGGTATTGCCAACCCTGCCCTAAACATTTACAATAATCCGGTGAATAAATTCGTGTTTAATTTCATTGGGCTATCCAATCAGTTGAACGTCAAttt                                                                         >  1:966945/1‑251 (MQ=255)                                                                                                                                                                           tCATTAATCCGATTCCCGATTTCGACGGTTCTTTTA‑CGCCGATACGCTTAGGCACATTAGGTATCGCCTACAACACCCGCTTTGTGAAAAAAGAACCGCCGGCACAATGGGCGGATATTCTTACTc                                                                                                                                                                                            >  1:716817/1‑126 (MQ=255)                                                                                                                                                                           tCATTAATCCGATTCCCGATTTCGACGGTTCTTTTA‑CGCCGATACGCTTAGGCACATTAGGTATCGCCTACAACACCCGCTTTGTGAAAAAAGAACCGCCGGCACAATGGGCGGATATTCTTACTCCGCAATTTAAAGGCGCATTCGGTATTGCCAACCCTGCCCTAAACATTTACAATAATCCGGTGAATAAATTCGTGTTTAATTTCATTGGGCTATCCAATCAGTTGAACGTCAATTTGTCTGCGCaa                                                               >  1:192092/1‑251 (MQ=255)                                                                                                                                                                                          ccgttttccgacgtttttttACCGCCGATACGCTAGGGCACTTTAGATATCGCCTACACCCCCCGTTTTGAAAAAAAAGAACCGCCGGCACAATGGGCGGATATTCTTACTCCGCAATTTAAAGGCGCATTCGGTATTGCCAACCCTGCCCTAAACATTTACAATAATCCGGTGAATAAATTCGTGTTTAATTTCATTGGGCTATCCAATCAGTTGAACGTCAATTTGTCTGCGCAAGGTGTCACTATTGa                                                 <  1:990783/235‑1 (MQ=255)                                                                                                                                                                                           cgattccgccgtttCTTTTC‑CGCCGATCCGCTTAGACACATTGGTTACCGCCACCACCCCCCGTTTTGAAAAAAAACAGCCGCCGGCACAATGGGCGGATATTCTTACTCCGCAATTTAAAGGCGCATTCGGTATTGCCAACCCTGCCCTAAACATTTACAATAATCCGGTGAATAAATTCGTGTTTAATTTCATTGGGCTATCCAATCAGTTGAACGTCAATTTGTCTGCGCAAGGTGTCACTATTGATc                                               <  1:96935/239‑1 (MQ=255)                                                                                                                                                                                           catttccgccGGTTTTTTTC‑CGCCGACACCCTTAGGCACATTAGGTATCCCCTACACCACCCGTTTTATGAAAAAAGAACCGCCGGCACAATGGGCGGATATTCTTACTCCGCAATTTAAAGGCGCATTCGGTATTGCCAACCCTGCCCTAAACATTTACAATAATCCGGTGAATAAATTCGTGTTTAATTTCATTGGGCTATCCAATCAGTTGAACGTCAATTTGTCTGCGCAAGGTGTCACTATTGATc                                               <  1:602066/242‑1 (MQ=255)                                                                                                                                                                                           cGATTTCGACGTTTCTTTTA‑CGCCGATACTCTTAGCCACATTGGGTCGCGCTTACACCCCCCGCTTTGAAAAAAAAGAACCGCCGGCACAATGGGCGGATATTCTTACTCCGCAATTTAAAGGCGCATTCGGTATTGCCAACCCTGCCCTAAACATTTACAATAATCCGGTGAATAAATTCGTGTTTAATTTCATTGGGCTATCCAATCAGTTGAACGTCAATTTGTCTGCGCAAGGTGTCACTATTGATc                                               <  1:528563/251‑1 (MQ=255)                                                                                                                                                                                                    gaaatgtgtaaaagagacagcGCTGAGGCAAATTAGGTATCCCCAACAACACCCGTTTTGAAAAAAAAAAACCGCCGGCACAATGGGCGGATATTCTTACTCCGCAATTTAAAGGCGCATTCGGTATTGCCAACCCTGCCCTAAACATTTACAATAATCCGGTGAATAAATTCGTGTTTAATTTCATTGGGCTATCCAATCAGTTGAACGTCAATTTGTCTGCGCAAGGTGTCACTATTGATCATTGTGa                                        <  1:4840/230‑1 (MQ=255)                                                                                                                                                                                                      gTTCTTTTA‑CGCCGATACGCTTAGGCACATTAGGTATCGCCTACAACACCCGCTTTGTGAAAAAAGAACCGCCGGCACAATGGGCGGATATTCTTACTCCGCAATTTAAAGGCGCATTCGGTATTGCCAACCCTGCCCTAAACATTTACAATAATCCGGTGAATAAATTCGTGTTTAATTTCATTGGGCTATCCAATCAGTTGAACGTCAATTTGTCTGCGCAAGGTGTCACTATTGATCATTGTGAAGgt                                    >  1:944790/1‑251 (MQ=255)                                                                                                                                                                                                      gTTCTTTTA‑CGCCGATACGCTTAGGCACATTAGGTATCGCCTACAACACCCGCTTTGTGAAAAAAGAACCGCCGGCACAATGGGCGGATATTCTTACTCCGCAATTTAAAGGCGCATTCGGTATTGCCAACCCTGCCCTAAACATTTACAATAATCCGGTGAATAAATTCGTGTTTAATTTCATTGGGCTATCCAATCAGTTGAACGTCAATTTGTCTGCGCAAGGTGTCACTATTGATCATTGTGAAGgt                                    >  1:579080/1‑251 (MQ=255)                                                                                                                                                                                                         tttttA‑CGCCGATCCGCTAAGGCACTTTAGGTATCGCCTACACCCCCCGTTTTGAAAAAAAAGAACCGCCGGCACAATGGGCGGATATTCTTACTCCGCAATTTAAAGGCGCATTCGGTATTGCCAACCCTGCCCTAAACATTTACAATAATCCGGTGAATAAATTCGTGTTTAATTTCATTGGGc                                                                                                  <  1:646268/185‑1 (MQ=255)                                                                                                                                                                                                               ccgctattactgtgagactcaTTAGGAATCCCCTACAACCCCCGTTTGGAAAAAAAAGAACCGCCGGCACAATGGGCGGATATTCTTACTCCGCAATTTAAAGGCGCATTCGGTATTGCCAACCCTGCCCTAAACATTTACAATAATCCGGTGAATAAATTCGTGTTTAATTTCATTGGGCTATCCAATCAGTTGAACGTCAATTTGTCTGCGCAAGGTGTCACTATTGATCATTGTGAAGGTGTTTTTaa                            <  1:441055/232‑1 (MQ=255)                                                                                                                                                                                                                  ccGATACGCTTAGGCACATTAGGTATCGCCTACAACACCCGCTTTGTGAAAAAAGAACCGCCGGCACAATGGGCGGATATTCTTACTCCGCAATTTAAAGGCGCATTCGGTATTGCCAACCCTGCCCTAAACATTTACAATAATcc                                                                                                                                  >  1:125157/1‑146 (MQ=255)                                                                                                                                                                                                                  ccGATACGCTTAGGCACATTAGGTATCGCCTACAACACCCGCTTTGTGAAAAAAGAACCGCCGGCACAATGGGCGGATATTCTTACTCCGCAATTTAAAGGCGCATTCGGTATTGCCAACCCTGCCCTAAACATTTACAATAATCCGGTGAATAAATTCGTGTTTAATTTCATTGGGCTATCCAATCAGTTGAACGTCAATTTGTCTGCGCAAGGTGTCACTATTGATc                                               >  1:634657/1‑229 (MQ=255)                                                                                                                                                                                                                       aCGCTTAGGCACATTAGGTATCGCCTACAACACCCGCTTTGTGAAAAAAGAACCGCCGGCACAATGGGCGGATATTCTTACTCCGCAATTTAAAGGCGCATTCGGTATTGCCAACCCTGCCCTAAACATTTACAATAATCCGGTGAATAAATTCGTGTTTAATTTCATTGGGCTATCCAATCAGTTGAACGTCAATTTGTCTGCGCAAGGTGTCACTa                                                     >  1:474929/1‑218 (MQ=255)                                                                                                                                                                                                                       aCGCTTAGGCACATTAGGTATCGCCTACAACACCCGCTTTGTGAAAAAAGAACCGCCGGCACAATGGGCGGATATTCTTACTCCGCAATTTAAAGGCGCATTCGGTATTGCCAACCCTGCCCTAAACATTTACAATAATCCGGTGAATAAATTCGTGTTTAATTTCATTGGGCTATCCAATCAGTTGAACGTCAATTTGTCTGCGCAAGGTGTCACTATTGATc                                               >  1:231321/1‑224 (MQ=255)                                                                                                                                                                                                                        tgaataagagaaagaGGTATCGCCTACAACCCCCGTTTTGTAAAAAAAAAACCGCCGGCACAATGGGCGGATATTCTTACTCCGCAATTTAAAGGCGCATTCGGTATTGCCAACCCTGCCCTAAACATTTACAATAATCCGGTGAATAAATTCGTGTTTAATTTCATTGGGCTATCCAATCAGTTGAACGTCAATTTGTCTGCGCAAGGTGTCACTATTGATCATTGTGAAGGTGTTTTTAATATTCAAc                    <  1:728144/236‑1 (MQ=255)                                                                                                                                                                                                                        cGCTTAGGCACATTAGGTATCGCCTACAACACCCGCTTTGTGAAAAAAGAACCGCCGGCACAATGGGCGGATATTCTTACTCCGCAATTTAAAGGCGCATTCGGTATTGCCAACCCTGCCCTAAACATTTACAATAATCCgg                                                                                                                                >  1:308255/1‑142 (MQ=255)                                                                                                                                                                                                                        cGCTTAGGCACATTAGGTACCGCCTACAACCCCCGTTTTGTAAAAAAAGAACCGCCGGCACAATGGGCGGATATTCTTACTCCGCAATTTAAAGGCGCATTCGGTATTGCCAACCCTGCCCTAAAc                                                                                                                                                <  1:322590/126‑1 (MQ=255)                                                                                                                                                                                                                          cTTAGGCACATTAGGTATCGCCTACAACACCCGCTTTGTGAAAAAAGAACCGCCGGCACAATGGGCGGATATTCTTACTCCGCAATTTAAAGGCGCATTCGGTATTGCCAACCCTGCCCTAAACATTTACAATAATCCGGTGAATAAATTCGTGTTTAATTTCATTGGGCTATCCAATCAGTTGAACGTCAATTTg                                                                        >  1:144808/1‑196 (MQ=255)                                                                                                                                                                                                                               gCACATTAGGTACCGCCTACAACCCCCGTTTTGTAAAAAAAAAACCGCCGGCACAATGGGCGGATATTCTTACTCCGCAATTTAAAGGCGCATTCGGTATTGCCAACCCTGCCCTAAACATTTACAATAATCCGGTGAATAAATTCGTGTTTAATTTCATTGGGCTATCCAATCAGTTGAACGTCAATTTGTCTGCGCAAGGTGTCACTATTGATCATTGTGAAGGTGTTTTTAATATTCAACCGACGcc             <  1:179515/250‑1 (MQ=255)                                                                                                                                                                                                                                 acaTTAGGTATCGCCTACAACACCCGCTTTGTGAAAAAAGAACCGCCGGCACAATGGGCGGATATTCTTACTCCGCAATTTAAAGGCGCATTCGGTATTGCCAACCCTGCCCTAAACATTTACAATAATCCGGTGAATAAATTCGTGTTTAATTTCATTGGGCTATCCAATCAGTTGAACGTCAATTTGTCTGCGCAAGGTGTCACTATTGATCATTGTGAAGGTGTTTTTAATATTCAACCGACGCCTc           >  1:976786/1‑250 (MQ=255)                                                                                                                                                                                                                                  caTTAGGTATCGCCTACAACACCCGCTTTGTGAAAAAAGAACCGCCGGCACAATGGGCGGATATTCTTACTCCGCAATTTAAAGGCGCATTCGGTATTGCCAACCCTGCCCTAAACATTTACAATAATCCGGTGAATAAATTCGTGTTTAATTTCATTGGGCTATCCAATCAGTTGAACGTCAATTTGTCTGCGCAAGGTGTCACTATTGATCATTGTGAAGGTGTTTTTAATATTCAACCGACGCCTCCg         >  1:269817/1‑251 (MQ=255)                                                                                                                                                                                                                                       ggTATCGCCTACAACACCCGCTTTGTGAAAAAAGAACCGCCGGCACAATGGGCGGATATTCTTACTCCGCAATTTAAAGGCGCATTCGGTATTGCCAACCCTGCCCTAAACATTTACAATAATCCGGTGAATAAATTCGTGTTTAATTTCATTGGGCTATCCAATCAGTTGAACGTCAATTTGTCTGCGCAAGGTGTCACTATTGATCATTGTGAAGGTGTTTTTAATATTCAACCGACGCCTCCGGCAGa    >  1:103070/1‑251 (MQ=255)                                                                                                                                                                                                                                         taccgcctccaccccccGTTTTGTAAAAAAAGAACCGCCGGCACAATGGGCGGATATTCTTACTCCGCAATTTAAAGGCGCATTCGGTATTGCCAACCCTGCCCTAAACATTTACAATAATCCGGTGAATAAATTCGTGTTTAATTTCATTGGGCTATCCAATCAGTTGAACGTCAATTTGTCTGCGCAAGGTGTCACTATTGATCATTGTGAAGGTGTTTTTAATATTCAACCGACGCCTCCGGCAGAat  <  1:341218/237‑1 (MQ=255)                                                                                                                                                                                                                                             gCCTACAACACCCGCTTTGTGAAAAAAGAACCGCCGGCACAATGGGCGGATATTCTTACTCCGCAATTTAAAGGCGCATTCGGTATTGCCAACCCTGCCCTAAACATTTACAATAATCCGGTGAATAAATTCGTGTTTAATTTCATTGGGCTATCCAATCAGTTGAACGTCAAtt                                                                          >  1:994644/1‑175 (MQ=255)                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                         ATGGACTATCAATCTGCCGGTGCGGGCAAACTCATGGCAAAAATTGCCACGGAAAAAGAATCCGGTCAGATTATGGCAGATGTTATTTGGA‑CCAGTGAAGTCCCTGACTTCTTCAATATGAAAGAAACCGGCATGCTAGAAACTTATATTTCGCCCGAAGTAGAAAATATCATTAATCCGATTCCCGATTTCGACGGTTCTTTTA‑CGCCGATACGCTTAGGCACATTAGGTATCGCCTACAACACCCGCTTTGTGAAAAAAGAACCGCCGGCACAATGGGCGGATATTCTTACTCCGCAATTTAAAGGCGCATTCGGTATTGCCAACCCTGCCCTAAACATTTACAATAATCCGGTGAATAAATTCGTGTTTAATTTCATTGGGCTATCCAATCAGTTGAACGTCAATTTGTCTGCGCAAGGTGTCACTATTGATCATTGTGAAGGTGTTTTTAATATTCAACCGACGCCTCCGGCAGAAT  >  1/1405121‑1405601

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑