| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | 1 | 1,287,522 | 0 | A | C | 37.8% | 153.3 / 16.8 | 75 | intergenic (+148392/+105789) | unknown/unknown | tRNA‑Met‑CAT/tRNA‑Val‑GAC |
| Reads supporting (aligned to +/- strand): ref base A (35/11); new base C (0/28); total (35/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.32e-11 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CGAAACACAGCGCGAGCAATTGCGTCAGGTTCAAGCGCAATATGCACAATACAATCAAGTGTTGATTCAATTGCAAAGTTCTTTCAACAGTAAAAACCAAATGTTGCAGGAATTAATGCAGGAAATCGGCGAAT‑TGGGTGTACGTGCCGACGAAGGCGCGGAAGAACGTGCAAAAATCCGTCGGGATGAGTTGTATCAACAACTTTCCACTAATCGTCAGTGCCGTTCTTACATTGAAAAACAACTCACCCTCATTGAAA‑GCGAAGCGGAAAA‑TCTTACCCGCCGCATTCGTAAAGCGGAACGGGACTACAAAACCCAGCGCGAATTGGTAACGGCGGCTAAAATGAGCTGGTGCGTGGTGCTGCGTTTATCTCGCAATTCTGATGTGGAAAAACGCTTAAATCGTCGTGAATTGGCATATTTATCCGCCGATGAGCTACGTTCCATGTCGGACAAAGCCCTTGGTGCGTTGCGTACGGCGGTTGCCGATAAC > 1/1287275‑1287767 | cgAAACACAGCGCGAGCAATTGCGTCAGGTTCAAGCGCAATATGCACAATACAATCAAGTGTTGATTCAATTGCAAAGTTCTTTCAACAGTAAAAACCAAATGTTGCAGGAATTAATGCAGGAAATCGGCGAAT‑TGGGTGTACGTGCCGACGAAGGCGCGGAAGAACGTGCAAAAATCCGTCGGGATGAGTTGTATCAACAACTTTCCACTAATCGTCAGTGCCGTTCTTACATTGAAAAACAACTCAcc < 1:485613/250‑1 (MQ=255)cgAAACACAGCGCGAGCAATTGCGTCAGGTTCAAGCGCAATATGCACAATACAATCAAGTGTTGATTCAATTGCAAAGTTCTTTCAACAGTAAAAACCAAATGTTGCAGGAATTAATGCAGGAAATCGGCGAAT‑TGGGTGTACGTGCCGACGAAGGCGCGGAAGAACGTGCAAAAATCCGTCGGGATGAGTTGTATCAACAACTTTCCACTAATCGTCAGTGCCGTTCTTACATTGAAAAACAACTCAcc < 1:401387/250‑1 (MQ=255)cgAAACACAGCGCGAGCAATTGCGTCAGGTTCAAGCGCAATATGCACAATACAATCAAGTGTTGATTCAATTGCAAAGTTCTTTCAACAGTAAAAACCAAATGTTGCAGGAATTAATGCAGGAAATCGGCGAAT‑TGGGTGTACGTGCCGACGAAGGCGCGGAAGAACGTGCAAAAATCCGTCGGGATGAGTTGTATCAACAACTTTCCACTAATCGTCAGTGCCGTTCTTACATTGAAAAACAACTCAcc < 1:114956/250‑1 (MQ=255) cgcgAGCAATTGCGTCAGGTTCAAGCGCAATATGCACAATACAATCAAGTGTTGATTCAATTGCAAAGTTCTTTCAACAGTAAAAACCAAATGTTGCAGGAATTAATGCAGGAAATCGGCGAAT‑TGGGTGTACGTGCCGACGAAGGCGCGGAAGAACGTGCAAAAATCCGTCGGGATGAGTTGTATCAACAACTTTCCACTAATCGTCAGTGCCGTTCTTACATTGAAAAACAACTCACCCTCATTg < 1:901689/247‑1 (MQ=255) cAATATGCACAATACAATCAAGTGTTGATTCAATTGCAAAGTTCTTTCAACAGTAAAAACCAAATGTTGCAGGAATTAATGCAGGAAATCGGCGAAT‑TGGGTGTACGTGCCGACGAAGGCGCGGAAGAACGTGCAAAAATCCGTCGGGATGAGTTGTATCAACAACTTTCCACTAATCGTCAGTGCCGTTCTTACATTGAAAAACAACTCACCCTCATTGAAA‑GCGAAGCGGAAAA‑TCTTACCCGCCGCAt > 1:44369/1‑251 (MQ=255) cAATATGCACAATACAATCAAGTGTTGATTCAATTGCAAAGTTCTTTCAACAGTAAAAACCAAATGTTGCAGGAATTAATGCAGGAAATCGGCGAAT‑TGGGTGTACGTGCCGACGAAGGCGCGGAAGAACGTGCAAAAATCCGTCGGGATGAGTTGTATCAACAACTTTCCACTAATCGTCAGTGCCGTTCTTACATTGAAAAACAACTCACCCTCATTGAAA‑GCGAAGCGGAAAA‑TCTTACCCGCCGCAt > 1:3702/1‑251 (MQ=255) cAATATGCACAATACAATCAAGTGTTGATTCAATTGCAAAGTTCTTTCAACAGTAAAAACCAAATGTTGCAGGAATTAATGCAGGAAATCGGCGAAT‑TGGGTGTACGTGCCGACGAAGGCGCGGAAGAACGTGCAAAAATCCGTCGGGATGAGTTGTATCAACAACTTTCCACTAATCGTCAGTGCCGTTCTTACATTGAAAAACAACTCACCCTCATTGAAA‑GCGAAGCGGAAAA‑TCTTACCCGCCGCAt > 1:330311/1‑251 (MQ=255) cAATATGCACAATACAATCAAGTGTTGATTCAATTGCAAAGTTCTTTCAACAGTAAAAACCAAATGTTGCAGGAATTAATGCAGGAAATCGGAGAAT‑TGGGTGTACGTGCCGACGAAGGCGCGGAAGAACGTGCAAAAATCCGTCGGGATGAGTTGTATCAACAACTTTCCACTAATCGTCAGTGCCGTTCTTACATTGAAAAACAACTCACCCTCATTGAAA‑GCGAAGCGGAAAA‑TCTTACCCGCCGCAt > 1:1041961/1‑251 (MQ=255) atatGCACAATACAATCAAGTGTTGATTCAATTGCAAAGTTCTTTCAACAGTAAAAACCAAATGTTGCAGGAATTAATGCAGGAAATCGGCGAAT‑TGGGTGTACGTGCCGACGAAGGCGCGGAAGAACGTGCAAAAATCCGTCGGGATGAGTTGTATCAACAACTTTCCACTAATCGTCAGTGCCGTTCTTACATTGAAAAACAACTCACCCTCATTGAAA‑GCGAAGCGGAAAA‑TCTTACCCGCCGCAtt > 1:182946/1‑250 (MQ=255) atatGCACAATACAATCAAGTGTTGATTCAATTGCAAAGTTCTTTCAACAGTAAAAACCAAATGTTGCAGGAATTAATGCAGGAAATCGGCGAAT‑TGGGTGTACGTGCCGACGAAGGCGCGGAAGAACGTGCAAAAATCCGTCGGGATGAGTTGTATCAACAACTTTCCACTAATCGTCAGTGCCGTTCTTACATTGAAAAACAACTCACCCTCATTGAAA‑GCGAAGCGGAAAA‑TCTTACCCGCCGCAtt > 1:98253/1‑250 (MQ=255) atGCACAATACAATCAAGTGTTGATTCAATTGCAAAGTTCTTTCAACAGTAAAAACCAAATGTTGCAGGAATTAATGCAGGAAATCGGCGAAT‑TGGGTGTACGTGCCGACGAAGGCGCGGAAGAACGTGCAAAAATCCGTCGGGATGAGTTGTATCAACAACTTTCCACTAATCGTCAGTGCCGTTCTTACATTGAAAAACAACTCACCCTCATTGAaa‑g > 1:917069/1‑220 (MQ=255) caatacaatCAAGTGTTGATTCAATTGCAAAGTTCTTTCAACAGTAAAAACCAAATGTTGCAGGAATTAATGCAGGAAATCGGCGAAT‑TGGGTGTACGTGCCGACGAAGGCGCGGAAGAACGTGCAAAAATCCGTCGGGATGAGTTGTATCAACAACTTTCCACTAATCGTCAGTGCCGTTCTTACATTGAAAAACAACTCACCCTCATTGAAA‑GCGAAGCGGAAAA‑TCTTACCCGCCGCATTCGTAAAg > 1:364884/1‑250 (MQ=255) cAAGTGTTGATTCAATTGCAAAGTTCTTTCAACAGTAAAAACCAAATGTTGCAGGAATTAATGCAGGAAATCGGCGAAT‑TGGGTGTACGTGCCGACGAAGGCGCGGAAGAACGTGCAAAAATCCGTCGGGATGAGTTGTATCAACAACTTTCCACTAATCGTCAGTGCCGTTCTTACATTGAAAAACAACTCACCCTCATTGAAA‑GCGAAGCGGAAAA‑TCTTACCCGCCGCATTCGTAAAGCGGAACGGGa > 1:735473/1‑251 (MQ=255) aaGTGTTGATTCAATTGCAAAGTTCTTTCAACAGTAAAAACCAAATGTTGCAGGAATTAATGCAGGAAATCGGCGAAT‑TGGGTGTACGTGCCGACGAAGGCGCGGAAGAACGTGCAAAAATCCGTCGGGATGAGTTGTATCAACAACTTTCCACTAATCGTCAGTGCCGTTCTTACATTAAAAAACAACTCCCCCTCATTGAAA‑GCGAAGCGGAAAA‑TCTTACCCGCCGCATTCGTAAAGCGGAACGGGAc < 1:18840/251‑1 (MQ=255) aaGTGTTGATTCAATTGCAAAGTTCTTTCAAAAGTAAAAACCAAATGTTGCAGGAATTAATGCAGGAAATCGGGGATT‑TGGGTGTACGTGCCGACGAAGGCGCGGAAGAACGTGAAAAAATCCGTGGGGATGGTTTGTATCAACAACTTTCCCCTAATCGTCAGGGCCGTTTTTACTTTGAAAACCAACTCTCCCTCATGGAAA‑GCGAAGCGAAAAA‑TCTTCCCCGCCGCATTCGTAAAGCGGAACGGGAc < 1:1060487/251‑1 (MQ=255) ttgtttCAATTGAAAAGTTTTTACAAAAGTAAAAACCAAATGTTGCAGAAATAAAGGCAGGAAATCGGCGATT‑TGGGTGTACGTGCCGACGAGGGCGCGGAAGAACGTGCAAAAATCCGTCGGGATGATTTGTATCAACAACTTCCCCCAAATCGTCAGTGCCGTTCTTACATTGAAAAACACCTCCCCCTCATTGAAA‑GCGAAGCGAAAAA‑TCTTCCCCGCCGCATTCGTAAAGCGGAACGGGACTACaa < 1:724516/247‑1 (MQ=255) ttaaTTCAATTGAAAATTTCTTTCAACAGTAAAAACCAAATGTTGCAGAAATTAATGCAGGAAATCGGCGAAT‑TGGGTGTACGTGCCGACGAAGGCGCGGAAGACCGTGAAAAAATCCGTGGGGATGATTTGTATCAACAACTTTCCACTAATCGTCAGTGCCTTTCTTACATTAAAAAACACCCCCCCCTCATTGAAA‑GCGAAGCGAAAAA‑TCTTACCCGCCGCATTCGTAAAGCGGAACGGGACTACaa < 1:476448/248‑1 (MQ=255) ttGATTCAATTGCAAAGTTCTTTCAACAGTAAAACCCAAATGTTGCAGGAATTAATGCAGGAAATCGGCGAAT‑GGGGTGTACGTGCCGACGAAGGCGCGGAAGAACGTGCAAAAACCCGTCGGGATGAGTTGTATCAACAACTTTCCACTAATCGTCAGTGCCGTTCTTACATTAAAAAACAACTCCCCCTCATTGAAA‑GCGAAGCGGAAAA‑TCTTACCCGCCGCATTCGTAAAGCGGAACGGGACTACaa < 1:219894/251‑1 (MQ=255) ttGATTCAATTGCAAAGTTCTTTCAACAGAAAAAACCAAATGTTGCAGGAATTAATGCAGAAAATCGGCGAAT‑GGGGTGTACGTGCCGACGAAGGCGCGGAAGAACGTGCAAAAACCCGTGGGGATGAGTTGTATCAACACCTTTCCACTAATCGTCAGTGCCGTTCTTACATTAAAAAACAACTCCCCCTCATGAAAA‑GCGAAGCGAAAAA‑TCTTACCCGCCGCATTCGTAAAGCGGAACGGGACTACaa < 1:202635/251‑1 (MQ=255) taaaTTGAAAAGTTCTTTAAACAGTAAAAACCAAATGTTGCAGAAATTAATGCAGGAAATCGGCGAAT‑GGGGTGTACGTGCCGACGAAGGCGCGGAAGAACGTGCAAAAACCCGTCGGGATGAGTTGTATCAACAACTTTCCACTAATCGTCAGTGCCGTTCTTACATTAAAAAACACCCCCCCCTCATTGAAA‑GCGAAGGGAAAAA‑TCTTACCCGCCGCATTCGTAAAGCGGAACGGGACTACAAAAccc < 1:764228/249‑1 (MQ=255) gTTCTTTCAACAGTAAAAACCAAATGTTGCAGGAATTAATGCAGGAAATCGGCGAAT‑TGGGTGTACGTGCCGACGAAGGCGCGGAAGAACGTGCAAAAATCCGTCGGGATGAGTTGTATCAACAACTTTCCACTAATCGTCAGTGCCGTTCTTACATTGAAAAACAACTCACCCTCATTGAAA‑GCGAAGCGGAAAA‑Tc < 1:612061/198‑1 (MQ=255) gTTCTTTCAACAGTAAAAACCAAATGTTGCAGGAATTAATGCAGGAAATCGGCGAAT‑TGGGTGTACGTGCCGACGAAGGCGCGGAAGAACGTGCAAAAATCCGTCGGGATGAGTTGTATCAACAACTTTCCACTAATCGTCAGTGCCGTTCTTACATTGAAAAACAACTCACCCTCATTGAAA‑GCGAAGCGGAAAA‑TCTTACCCGCCGCATTCGTAAAGCGGAACGGGACTACAAAACCCAGCGCGAATTg > 1:598198/1‑251 (MQ=255) ttCTTTCAACAGTAAAAACCAAATGTTGCAGGAATTAATGCAGGAAATCGGCGAAT‑TGGGTGTACGTGCCGACGAAGGCGCGAAAGACCGTGAAAAAACCCGTCGGGATGAGTTGTATCAACAACTTTCCACTAATCGTCAGTGCCGTTCTTACATTAAAAAACAACTCCCCCTCATTGAAA‑GCGAAGCGGAAAA‑TCTTACCCGCCGCATTCGTAAAGCGGAACGGGACTACAAAACCCAGCGCGAAtt < 1:363275/249‑1 (MQ=255) ttttCAACAGAAAAACCCAAATGTTGCAGAAATTAAGGAAGAAAATCGGCGAAT‑TGGGTGTCCGTGCCGACGAAGGCGCGGAAGAACGTGCAAAAATCCGTCGGGATGAGTTGTATCAACACCTTCCCACTAATCGTCAGTGCCGTTCTTACATTAAAAAACAACTCCCCCTCATTGAAA‑GCGAAGCGGAAAA‑TCTTACCCGCCGCATTCGTAAAGCGGAACGGGACTACAAAACCCAGCGCGAATTGGTa < 1:40507/250‑1 (MQ=255) ataTTAATGCAGAACATCGGC‑AATGTGGGTGTAAGAGCCGCCGAGGGCGCGGAAGAACGTGAAAAATTCCGTGGGGAGGAGGTATAACAAAAATTTTCCACTAATCGTCTGTGCCTTTTTTACATGGAAAAACAACCCACCCTCATTGAAA‑GCGAATGGTAAAT‑TTTTCCCCGCCGCATTCGTAAAGCGGAACGGGACTACAAAACCCAGCGCGAATTGGTAACGGCGGCTAAAATGAGCTGGTGCgt < 1:17184/246‑1 (MQ=255) aaTTAATGCAGGAAATCGGCGAAT‑TGGGTGTAGGTGCCGACGAAGGCGCGGAAGAACGTGAAAAAATCCGTCGGGATGAGTTGTATCAACAACTTTCCACTAATCGTCAGTGCCGTTCTTACATTAAAAAACAACCCCCCCTCATTGAAA‑GCGAAGCGGAAAA‑TCTTCCCCGCCGCATTCGTAAAGCGGAACGGGACTACAAAACCCAGCGCGAATTGGTAACGGCGGCTAAAATGAGCTGGTGCgtggtg < 1:977275/251‑1 (MQ=255) aTTAATGCAGGAAATCGGCGAAT‑TGGGTGTACGTGCCGACGAAGGCGCGGAAGAACGTGAAAAAATCCGTCGGGATGAGTTGTATCAACAACTTTCCACTAATCGTCAGTGCCGTTCTTACATTGAAAAACAACTCCCCCTCATTGAAA‑GCGAAGCGGAAAA‑TCTTACCCGCCGCATTCGTAAAGCGGAACGGGACTACAAAACCCAGCGCGAATTGGTAACGGCGGCTAAAATGAGCTGGTGCGTGGtgc < 1:817162/251‑1 (MQ=255) tGCAGGAAATCGGCGAAT‑TGGGTGTACGTGCCGACGAAGGCGCGAAAGAACGTGCAAAAATCCGTCGGGATGAGTTGTATCAACAACTTTCCACTAATCGTCAGTGCCTTTCTTACATTGAAAAACAACTCCCCCTCATTGAAA‑GCGAAGCGGAAAA‑TCTTCCCCGCCGCATTCGTAAAGCGGAACGGGACTACAAAACCCAGCGCGAATTGGTAACGGCGGCTAAAATGAGCTGGTGCGTGGTGCTGCGt < 1:345424/251‑1 (MQ=255) gCAGAAAACCGGCGAAT‑TGGGTGTACGTGCCGAGGAAGGCGCGAAAGAACGTAAAAAAACCCGGCGGGATGATTTGTATAAACACCTTCCCACTAATCGTCAGTGCCTTTCTTACATTAAAAAACAACCCCCCCCCATTAAAA‑GCGAAGCGAAAAA‑TCTTACCCGCCGCATTCGTAAAGCGGAACGGGACTACAAAACCCAGCGCGAATTGGTAACGGCGGCTAAAATGAGCTGGTGCGTGGTGCTGCGt < 1:748603/250‑1 (MQ=255) agatgtgtaaaagagacgggggTGTACGTGCCGACGAAGGCGCGGAAGAACGTGAAAAAATCCGTCGGGATGAGTTGTATCAACAACTTTCCACTAATCGTCAGTGCCGTTCTTACATTAAAAAACACCTCCCCCTCATTGAAA‑GCGAAGCGAAAAA‑TCTTCCCCGCCGCATTCGTAAAGCGGAACGGGACTACAAAACCCAGCGCGAATTGGTAACGGCGGCTAAAATGAGCTGGTGCGTGGTGCTGCg < 1:6960/231‑1 (MQ=255) cAGGAAATCGGCGAAT‑TGGGTGTACGTGCCGACGAAGGCGCGGAAGAACGTGCAAAAATCCGTCGGGATGAGTTGTATCAACAACTTTCCACTAATCGTCAGTGCCGTTCTTACATTGAAAAACAACTCACCCTCATTGAAA‑GCGAAGCGGAAAA‑TCTTAccc < 1:812643/163‑1 (MQ=255) ggAAATCGGCGAAT‑TGGGTGTACGTGCCGACGAAGGCGCGGAAGAACGTGCAAAAATCCGTCGGGATGAGTTGTATCAACAACTTTCCACTAATCGTCAGTGCCGTTCTTACATTGAAAAACAACTCACCCTCATTGAAA‑GCGAAGCGGAAAA‑TCTTACCCGCCGCATTCGTAAAGCGGAACGGGACTACAAAACCCAGCGCGAATTGGTAACGGCGGCTAAAATGAGCTGGTGCGTGGTGCTGCGTTTAt > 1:45546/1‑251 (MQ=255) aaT‑TGGGTGTACGTGCCGACGAAGGCGCGGAAGAACGTGCAAAAATCCGTCGGGATGAGTTGTATCAACAACTTTCCACTAATCGTCAGTGCCGTTCTTACATTGAAAAACAACTCACCCTCATTGAAA‑GCGAAGCGGAAAA‑TCTTACCCGCCGCATTCGTAAAGCGGAACGGGACTACAAAACCCAGCGCGAATTGGTAACGGCGGCTAAAATGAGCTGGTGCGTGGTGCTGCGTTTATCTCGCAAtt > 1:692813/1‑249 (MQ=255) aaT‑TGGGTGTACGTGCCGACGAAGGCGCGGAAGAACGTGCAAAAACCCGTGGGGATGAGTTGTATCAACAACTTTCCACTAATCGTCAGTGCCGTTCTTACATTAAAAAACAACTCACCCTCATTGAAA‑GCGAAGCGAAAAA‑TCTTCCCCGCCGCATTCGTAAAGCGGAACGGGACTACAAAACCCAGCGCGAATTGGTAACGGCGGCTAAAATGAGCTGGTGCGTGGTGCTGCGTTTATCTCGCAATTCt < 1:1065328/251‑1 (MQ=255) gggTGTACGTGCCGACGAAGGCGCGGAAGAACGTGCAAAAATCCGTCGGGATGAGTTGTATCAACAACTTTCCACTAATCGTCAGTGCCGTTCTTACATTGAAAAACAACTCACCCTCATTGAAA‑GCGAAGCGGAAAA‑TCTTAccc > 1:845975/1‑146 (MQ=255) gggTGTACGTGCCGACGAAGGCGCGGAAGAACGTGCAAAAATCCGTCGGGATGAGTTGTATCAACAACTTTCCACTAATCGTCAGTGCCGTTCTTACATTGAAAAACAACTCACCCTCATTGAAA‑GCGAAGCGGAAAA‑TCTTACCCGCCGCATTCGTAAAGCGGAACGGGACTACAAAACCCAGCGCGAATTGGTAACGGCGGCTAAAATGAGCTGGTGCGTGGTGCTGCGTTTATCTCGCAATTCTGa > 1:883040/1‑249 (MQ=255) cgacgaAGGCGCGGAAGAACGTGCAAAAATCCGTCGGGATGAGTTGTATCAACAACTTTCCACTAATCGTCAGTGCCGTTCTTACATTGAAAAACAACTCACCCTCATTGAAA‑GCGAAGCGGAAAA‑TCTTACCCGCCGCATTCGTAAAGCGGAACGGGACTACAAAACCCAGCGCGAATTGGTAACGGCGGCTAAAATGAGCTGGTGCGTGGTGCTGCGTTTATCTCGCAATTCTGATGTGGAAAAACg > 1:491004/1‑249 (MQ=255) gacgaAGGCGCGGAAGAACGTGCAAAAATCCGTCGGGATGAGTTGTATCAACAACTTTCCACTAATCGTCAGTGCCGTTCTTACATTGAAAAACAACTCACCCTCATTGAAA‑GCGAAGCGGAAAA‑TCTTACCCGCCGCATTCGTAAAGCGGAACGGGACTACAAAACCCAGCGCGAATTGGTAACGGCGGCTAAAATGAGCTGGTGCGTGGTGCTGCGTTTATCTCGCAATTCTGATGTGGAAAAACGCtt > 1:153453/1‑251 (MQ=255) aaGGCGCGGAAGACCGTGAAAAAACCCGTCGGGATGAGTTGTATCAACAACTTTCCACTAATCGTCAGTGCCTTTCTTACATTGAAAAACAACTCCCCCTCATAGAAA‑GCGAAGCGGAACA‑TCTTACCCGCCGCATTCGTAAAGCGGAACGGGACTACAAAACCCAGCGCGAATTGGTAACGGCGGCTAAAATGAGCTGGTGCGTGGTGCTGCGTTTATCTCGCAATTCTGATGTGGAAAAACGCTTAAAt < 1:568807/251‑1 (MQ=255) ggCGCGGAAGAACGTGCAAAAATCCGTCGGGATGAGTTGTATCAACAACTTTCCACTAATCGTCAGTGCCGTTCTTACATTGAAAAACAACTCACCCTCATTGAAA‑GCGAAGCGGAAAA‑TCTTACCCGCCGCATTCGTAAAGCGGAACGGGACTACAAAACCCAGCGCGAATTGGTAACGGCGGCTAAAATGAGCTGGTGCGTGGTGCTGCGTTTATCTCGCAATTCTGATGTGGAAAAACGCTTAAAtcg > 1:671615/1‑251 (MQ=255) ggCGCGGAAGAACGTGCAAAAATCCGTCGGGATGAGTTGTATCAACAACTTTCCACTAATCGTCAGTGCCGTTCTTACATTGAAAAACAACTCACCCTCATTGAAA‑GCGAAGCGGAAAA‑TCTTACCCGCCGCATTCGTAAAGCGGAACGGGACTACAAAACCCAGCGCGAATTGGTAACGGCGGCTAAAATGAGCTGGTGCGTGGTGCTGCGTTTATCTCGCAATTCTGATGTGGAAAAACGCTTAAAtcg > 1:486800/1‑251 (MQ=255) gcgcGGAAGAACGTGCAAAAATCCGTCGGGATGAGTTGTATCAACAACTTTCCACTAATCGTCAGTGCCGTTCTTACATTGAAAAACAACTCACCCTCATTGAAA‑GCGAAGCGGAAAA‑TCTTACCCGCCGCATTCGTAAAGCGGAACGGGACTACAAAAccc > 1:178270/1‑162 (MQ=255) cGTGAAAAAATCGGTCGGGATGATTTATATCAACAACTTCCCACTAATCGTCAGTGCCTTTCTTACATTGAAAAACAACTCCCCCTCATTGAAA‑GCGAAGCGAAAAA‑TCTTACCCGCCGCATTCGTAAAGCGGAACGGGACTACAAAACCCAGCGCGAATTGGTAACGGCGGCTAAAATGAGCTGGTGCGTGGTGCTGCGTTTATCTCGCAATTCTGATGTGGAAAAACGCTTAAATCGTCGTGAATTGGc < 1:24675/251‑1 (MQ=255) aaaaTCCGTCGGGATGAGTTGTATCAACAACTTTCCACTATTCGCCAGTGCCGTTCTTACATTAAAAAACACCCCCCCCTCATTGAAA‑GCGAAGCGGAAAT‑TCTTCCCCGCCGCATTCGTAAAGCGGAACGGGACTACAAAACCCAGCGCGAATTGGTAACGGCGGCTAAAATGAGCTGGTGCGTGGTGCTGCGTTTATCTCGCAATTCTGATGTGGAAAAACGCTTAAATCGTCGTGAATTGGCATAttt < 1:539006/251‑1 (MQ=255) aaaaTCCGTCGGGATGAGTTGTATCAAAAACTTCCCACTAATCGTCAGTGCCGTTCTTACATTAAAAAACACCTCCCCCTCATTGAAA‑GCGAAGCGGAAAA‑TCTTCCCCGCCGCATTCGTAAAGCGGAACGGGACTACAAAACCCAGCGCGAATTGGTAACGGCGGCTAAAATGAGCTGGTGCGTGGTGCTGCGTTTATCTCGCAATTCTGATGTGGAAAAACGCTTAAATCGTCGTGAATTGGCATAttt < 1:250390/251‑1 (MQ=255) aaTCCGTCGGGATGAGTTGTATCAACAACTTTCCACTAAGCCTCGGTGCCGTTCTTACATAAAAAAGCAACCCACCCACATAGAAA‑GCGAGGGGGAAAA‑TCTTACCCGCCGCATTCGTAAAGCGGAACGGGACTACAAAACCCAGCGCGAATTGGTAACGGCGGCTAAAATGAGCTGGTGCGTGGTGCTGCGTTTATCTCGCAATTCTGATGTGGAAAAACGCTTAAATCGTCGTGAATTGGCATATTTAt < 1:818742/251‑1 (MQ=255) aTCCGTCGGGATGAGTTGTATCAACAATTTTCCACTAATCGTCAGGGCCGTTCTTACATTGAAAAACAACCCCCCCTCATTGAAA‑GCGAAGCGGAAAA‑TCTTACCCGCCGCATTCGTAAAGCGGAACGGGACTACAAAACCCAGCGCGAATTGGTAACGGCGGCTAAAATGAGCTGGTGCGTGGTGCTGCGTTTATCTCGCAATTCTGATGTGGAAAAACGCTTAAATCGTCGTGAATTGGCATATTTATc < 1:1078104/251‑1 (MQ=255) ccGTCGGGATGAGTTGTATCAACAACTTCCCACTAATCGTCAGTGCCTTTCTTACATTGAAAAACAACCCCCCCTCATTGAAA‑GCGAGGGGAAAAA‑TCTTACCCGCCGCATTCGTAAAGCGGAACGGGACTACAAAACCCAGCGCGAATTGGTAACGGCGGCTAAAATGAGCTGGTGCGTGGTGCTGCGTTTATCTCGCAATTCTGATGTGGAAAAACGCTTAAATCGTCGTGAATTGGCATATTTATccg < 1:628016/251‑1 (MQ=255) gggATGAGTTGTATCAACAACTTTCCACTAATCGTCAGTGCCGTTCTTACATTGAAAAACAACTCACCCTCATTGAAA‑GCGAAGCGGAAAA‑TCTTACCCGCCGCATTCGTAAAGCGGAACGGGACTACAAAACCCAGCGCGAATTGGTAACGGCGGCTAAAATGAGCTGGTGCGTGGTGCTGCGTTTATCTCGCAATTCTGATGTGGAAAAACGCTTAAAt > 1:290390/1‑221 (MQ=255) gggATGAGTTGTATCAACAACTTTCCACTAATCGTCAGTGCCGTTCTTACATTGAAAAACAACTCACCCTCATTGAAA‑GCGAAGCGGAAAA‑TCTTACCCGCCGCATTCGTAAAGCGGAACGGGACTACAAAACCCAGCGCGAATTGGTAACGGCGGCTAAAATGAGCTGGTGCGTGGTGCTGCGTTTATCTCGCAATTCTGATGTGGAAAAACGCTTAAATCGTCGTGAATTGGCATATTTATCCGCCGAt > 1:814442/1‑251 (MQ=255) ggATGAGTTGTATCAACAACTTTCCACTAATCGTCAGTGCCGTTCTTACATTGAAAAACAACTCACCCTCATTGAAA‑GCGAAGCGGAAAA‑TCTTACCCGCCGCATTCGTAAAGCGGAACGGGACTACAAAAccc > 1:719508/1‑134 (MQ=255) ggATGAGTTGTATCAACAACTTTCCACTAATCGTCAGTGCCGTTCTTACATTGAAAAACAACTCACCCTCATTGAAA‑GCGAAGCGGAAAA‑TCTTACCCGCCGCATTCGTAAAGCGGAACGGGACTACAAAACCCAGCGCGAATTGGTAACGGCGGCTAAAATGAGCTGGTGCGTGGGGCTGCGTTTATCTCGCAATTCTGATGTGGAAAAACGCTTAAATCGGCGTGAATTGGCATATTTATCCGCCGATg > 1:515594/1‑251 (MQ=255) gAGTTGTATCAACAACTTTCCACTAATCGTCAGTGCCGTTCTTACATTGAAAAACAACTCACCCTCATTGAAA‑GCGAAGCGGAAAA‑TCTTACCCGCCGCATTCGTAAAGCGGAACGGGACTACAAAACCCAGCGCGAATTGGTAACGGCGGCTAAAATGAGc > 1:503718/1‑162 (MQ=255) gAGTTGTATCAACAACTTTCCACTAATCGTCAGTGCCGTTCTTACATTGAAAAACAACTCACCCTCATTGAAA‑GCGAAGCGGAAAA‑TCTTACCCGCCGCATTCGTAAAGCGGAACGGGACTACAAAACCCAGCGCGAATTGGTAACGGCGGCTAAAATGAGCTGGTGCGTGGTGCTGCGTTTATCTCGCAATTCTGATGTGGAAAAACGCTTAAATCGTCGTGAATTGGCATATTTATCCGCCGATGAg > 1:635078/1‑249 (MQ=255) gTTGTATCAACAACTTTCCACTAATCGTCAGTGCCGTTCTTACATTGAAAAACAACTCACCCTCATTGAAA‑GCGAAGCGGAAAA‑TCTTACCCGCCGCATTCGTAAAGCGGAACGGGACTACAAAACCCAGCGCGAATTGGTAACGGCGGCTAAAATGAGCTGGTGCGTGGTGCTGCGTTTATCTCGCAATTCTGATGTGGAAAAACGCTTAAATCGTCGTGAATTGGCATATTTATCCGCCGATGAGCTa > 1:683332/1‑250 (MQ=255) aTCAACAACTTTCCACTAATCGTCAGTGCCGTTCTTACATTGAAAAACAACTCACCCTCATTGAAA‑GCGAAGCGGAAAA‑TCTTACCCGCCGCATTCGTAAAGCGGAACGGGACTACAAAACCCAGCGCGAATTGGTAACGGCGGCTAAAATGAGCTGGTGCGTGGTGCTGCGTTTATCTCGCAATTCTGATGTGGAAAAACGCTTAAATCGTCGTGAATTGGCATATTTATCCGCCGATGAGCTACGTTc > 1:989206/1‑250 (MQ=255) aTCAACAACTTTCCACTAATCGTCAGTGCCGTTCTTACATTGAAAAACAACTCACCCTCATTGAAA‑GCGAAGCGGAAAA‑TCTTACCCGCCGCATTCGTAAAGCGGAACGGGACTACAAAACCCAGCGCGAATTGGTAACGGCGGCTAAAATGAGCTGGTGCGTGGGGCTGCGTTTATCTCGCAATTCTGATGTGGAAAAACGCTTAAATCGTCGTGAATTGGCATATTTATCCGCCGATGAGCTACGTTc > 1:394829/1‑250 (MQ=255) caacaaCTTCCCACTAATCGTCAGTGCCTTTCTTACATTGAAAAACACCTCCCCCTCATTGAAA‑GCGAAGCGAAAAA‑TCTTACCCGCCGCATTCGTAAAGCGGAACGGGACTACAAAACCCAGCGCGAATTGGTAACGGCGGCTAAAATGAGCTGGTGCGTGGTGCTGCGTTTATCTCGCAATTCTGATGTGGAAAAACGCTTAAATCGTCGTGAATTGGCATAttt < 1:755957/227‑1 (MQ=255) caaaaccttcccACAAATCGTCAGGCCCGTTTTTACTTTAAAAAACAACCCCCCCACATTAAAG‑GCGAGGCGAAAAT‑TCTTCCCCGCCGCATTCGTAAAGCGGAACGGGACTACAAAACCCAGCGCGAATTGGTAACGGCGGCTAAAATGAGCTGGTGCGTGGTGCTGCGTTTATCTCGCAATTCTGATGTGGAAAAACGCTTAAATCGTCGTGAATTGGCATATTTATCCGCCGATGAGCTACGTTCCAt < 1:237134/241‑1 (MQ=255) aaacatcttcccccaaagcGTCAGGGCCGTTTTTCCATTAAAAACCACCCCACCCTCATTGAAA‑GGGAAG‑GGAAAATTCTTCCCCGCCGCATTCGTAAAGCGGAACGGGACTACAAAACCCAGCGCGAATTGGTAACGGCGGCTAAAATGAGCTGGTGCGTGGTGCTGCGTTTATCTCGCAATTCTGATGTGGAAAAACGCTTAAATCGTCGTGAATTGGCATATTTATCCGCCGATGAGCTACGTTCCAt < 1:340460/233‑1 (MQ=255) acatcttcccACTAATCGTCGGGGCCGTTTTTACATTAAAAAACACCCCCCCCTAATAAAAA‑GCGAGGCGAAAAA‑TCTTCCCCGCCGCATTCGTAAAGCGGAACGGGACTACAAAACCCAGCGCGAATTGGTAACGGCGGCTAAAATGAGCTGGTGCGTGGTGCTGCGTTTATCTCGCAATTCTGATGTGGAAAAACGCTTAAATCGTCGTGAATTGGCATATTTATCCGCCGATGAGCTACGTTCCATGt < 1:991848/243‑1 (MQ=255) acaacTTCCCATTAATCGTCAGTGCCTTTCTAACATAAAAAAACAACCCCCCCT‑AATGAAA‑AGGGAGGGGAAAATTCTTCCCCGCCGCATTCGTAAAGCGGAACGGGACTACAAAACCCAGCGCGAATTGGTAACGGCGGCTAAAATGAGCTGGTGCGTGGTGCTGCGTTTATCTCGCAATTCTGATGTGGAAAAACGCTTAAATCGTCGTGAATTGGCATATTTATCCGCCGATGAGCTACGTTCGATGt < 1:143088/251‑1 (MQ=255) caccTTTCCACTAATCGTCAGTGCCGTTCTTACATTGAAAAACAACCCACCCTCATTGAAA‑GCGAAGCGGAAAT‑TCTTACCCGCCGCATTCGTAAAGCGGAACGGGACTACAAAACCCAGCGCGAATTGGTAACGGCGGCTAAAATGAGCTGGTGCGTGGTGCTGCGTTTATCTCGCAATTCTGAtg < 1:847875/184‑1 (MQ=255) ctttcccACTAACCGTCAGTGCCTTTTTTACATTAAAAAACACCCCCCCCTCA‑TGAAA‑AGGGAGGGGAAAATTCTTCCCCGCCGCATTCGTAAAGCGGAACGGGACTACAAAACCCAGCGCGAATTGGTAACGGCGGCTAAAATGAGCTGGTGCGTGGTGCTGCGTTTATCTCGCAATTCTGATGTGGAAAAACGCTTAAATCGTCGTGAATTGGCATATTTATCCGCCGATGAGCTACGTTCCATGTCgg < 1:783359/246‑1 (MQ=255) tttCCACTAATCGTCAGTGCCGTTCTTAAATTAAAAAACACCCCCCCCTCATTGAAA‑GCGAAGCGAAAAA‑TCTTCCCCGCCGCATTCGTAAAGCGGAACGGGACTACAAAACCCAGCGCGAATTGGTAACGGCGGCTAAAATGAGCTGGTGCGTGGTGCTGCGTTTATCTCGCAATTCTGATGTGGAAAAACGCTTAAATCGTCGTGAATTGGCATATTTATCCGCCGATGAGCTACGTTCCATGTCGGAc < 1:394790/251‑1 (MQ=255) cTAATCGTCAGTGCCGTTCTTACATTGAAAAACAACTCACCCTCATTGAAA‑GCGAAGCGGAAAA‑TCTTACCCGCCGCATTCGTAAAGCGGAACGGGACTACAAAACCCAGCGCGAATTGGTAACGGCGGCTAAAATGAGCTGGTGCGTGGTGCTGCGTTTATCTCGCAATTCTGATGTGGAAAAACGCTTAAATCGTCGTGAATTGGCATATTTATCCGCCGATGAGCTACGTTCCATGTCGGACAAAGc > 1:882739/1‑250 (MQ=255) aaTCGTCAGTGCCGTTCTTACATTGAAAAACAACTCACCCTCATTGAAA‑GCGAAGCGGAAAA‑TCTTACCCGCCGCATTCGTAAAGCGGAACGGGACTACAAAACCCAGCGCGAATTGGTAACGGCGGCTAAAATGAGCTGGTGCGTGGTGCTGCGTTTATCTCGCAATTCTGATGTGGAAAAACGCTTAAATCGTCGTGAATTGGCATATTTATCCGCCGATGAGCTACGTTCCATGTCGGACAAAGcc > 1:828679/1‑249 (MQ=255) cggTGCCGTTCTTACATAAAAAAACAACCCCCCCTCA‑TGAAAGGCGAAGGGAAAAA‑TCTTCCCCGCCGCATTCGTAAAGCGGAACGGGACTACAAAACCCAGCGCGAATTGGTAACGGCGGCTAAAATGAGCTGGTGCGTGGTGCTGCGTTTATCTCGCAATTCTGATGTGGAAAAACGCTTAAATCGTCGTGAATTGGCATATTTATCCGCCGATGAGCTACGTTCCATGTCGGACAAAGCCCTTGGtgc < 1:406843/249‑1 (MQ=255) ccGTTCTTACATTAAAAAACACCTCCCCCTCATTAAAA‑GCGAAGCGGAAAA‑TCTTACCCGCCGCATTCGTAAAGCGGAACGGGACTACAAAACCCAGCGCGAATTGGTAACGGCGGCTAAAATGAGCTGGTGCGTGGTGCTGCg < 1:529954/144‑1 (MQ=255) gTTCTTACATTGAAAAACAACTCACCCTCATTGAAA‑GCGAAGCGGAAAA‑TCTTACCCGCCGCATTCGTAAAGCGGAACGGGACTACAAAACCCAGCGCGAATTGGTAACGGCGGCTAAAATGAGCTGGTGCGTGGTGCTGCGTTTATCTCGCAATTCTGATGTGGAAAAACGCTTAAATCGTCGTGAATTGGCATATTTATCCGCCGATGAGCTACGTTCCATGTCGGACAAAGCCCTTGGtgcgttgcgt > 1:421961/1‑251 (MQ=255) tACATTAAAAAACACCCCCCCCTCATGAAAA‑GCGAAGCGAAAAA‑TCTTCCCCGCCGCATTCGTAAAGCGGAACGGGACTACAAAACCCAGCGCGAATTGGTAACGGCGGCTAAAATGAGCTGGTGCGTGGTGCTGCGTTTATCTCGCAATTCTGATGTGGAAAAACGCTTAAATCGTCGTGAATTGGCATATTTATCCGCCGATGAGCTACGTTCCATGTCGGACAAAGCCCTTGGTGCGTTGCGTAcggc < 1:949388/251‑1 (MQ=255) gAAAAACAACTCACCCTCATTGAAA‑GCGAAGCGGAAAA‑TCTTACCCGCCGCATTCGTAAAGCGGAACGGGACTACAAAACCCAGCGCGAATTGGTAACGGCGGCTAAAATGAGCTGGTGCGTGGTGCTGCGTTTATCTCGCAATTCTGATGTGGAAAAACGCTTAAATCGTCGTGAATTGGCATATTTATCCGCCGATGAGCTACGTTCCATGTCGGACAAAGCCCTTGGTGCGTTGCGTACGGCGGTTgg > 1:902645/1‑250 (MQ=255) aaaaCAACTCACCCTCATTGAAA‑GCGAAGCGGAAAA‑TCTTACCCGCCGCATTCGTAAAGCGGAACGGGACTACAAAACCCAGCGCGAATTGGTAACGGCGGCTAAAATGAGCTGGTGCGTGGTGCTGCGTTTATCTCGCAATTCTGATGTGGAAAAACGCTTAAATCGTCGTGAATTGGCATATTTATCCGCCGATGAGCTACGTTCCATGTCGGACAAAGCCCTTGGTGCGTTGCGTACGGCGGTTggcg > 1:603134/1‑248 (MQ=255) aacTCACCCTCATTGAAA‑GCGAAGCGGAAAA‑TCTTACCCGCCGCATTCGTAAAGCGGAACGGGACTACAAAACCCAGCGCGAATTGGTAACGGCGGCTAAAATGAGCTGGTGCGTGGTGCTGCGTTTATCTCGCAATTCTGATGTGGAAAAACGCTTAAATCGTCGTGAATTGGCATATTTATCCGCCGATGAGCTACGCTCCATGTCGGACAAAGCCCTTGGTGCGTTGCGTACGGCGGTTGCCGGTaaa > 1:1013412/1‑250 (MQ=255) cACCCTCATTGAAA‑GCGAAGCGGAAAA‑TCTTACCCGCCGCATTCGTAAAGCGGAACGGGACTACAAAACCCAGCGCGAATTGGTAACGGCGGCTAAAACGGGCTGGTGCGAGGAGCCGCGTTTATCTCGCAATTCTGATGTGGAAAAACGCTTAAATCGTCGAGAATTGGCATATTTATCCGCCGATGAGCTACGTTCCATGTCGGACAAAGCCCTTGGTGCGTTGCGTAcggcgg > 1:864552/1‑236 (MQ=255) | CGAAACACAGCGCGAGCAATTGCGTCAGGTTCAAGCGCAATATGCACAATACAATCAAGTGTTGATTCAATTGCAAAGTTCTTTCAACAGTAAAAACCAAATGTTGCAGGAATTAATGCAGGAAATCGGCGAAT‑TGGGTGTACGTGCCGACGAAGGCGCGGAAGAACGTGCAAAAATCCGTCGGGATGAGTTGTATCAACAACTTTCCACTAATCGTCAGTGCCGTTCTTACATTGAAAAACAACTCACCCTCATTGAAA‑GCGAAGCGGAAAA‑TCTTACCCGCCGCATTCGTAAAGCGGAACGGGACTACAAAACCCAGCGCGAATTGGTAACGGCGGCTAAAATGAGCTGGTGCGTGGTGCTGCGTTTATCTCGCAATTCTGATGTGGAAAAACGCTTAAATCGTCGTGAATTGGCATATTTATCCGCCGATGAGCTACGTTCCATGTCGGACAAAGCCCTTGGTGCGTTGCGTACGGCGGTTGCCGATAAC > 1/1287275‑1287767 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |