| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | 1 | 1,129,501 | 0 | T | C | 38.3% | 107.2 / 15.5 | 60 | intergenic (+14011/‑9553) | unknown/unknown | tRNA‑Gln‑TTG/tRNA‑Met‑CAT |
| Reads supporting (aligned to +/- strand): ref base T (22/15); new base C (0/23); total (22/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.24e-07 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GATAACACAACAGATTATTGCGATAACGGTATGAGTCAGCAAATCTCCACCGAATGGGAGGCTAAGGCCAAAGCCGAATGGATATTCCAGCGTTTCTTTATGGGTTGAGGTAGATTTCTTAAAAAGCAAAAGATGCAAGGCATTAATAGATAAAGCCAGATCAACTGCCGACGAAATCATTAGAGACCTAAAAAATTTAAAAGATACTATTTTGTCCGTTGATACAGACAAA‑GCGTTTC‑TAATAATTTTCCCACAGTGGGAATCGCAGCTAC‑TGGAAAG‑TTTGCCGCCCCGTAAAGCGGGACTACCTGCTACTCCCGCCGATGTATCTTATCTAGACAAATACAAGCCAAAGAAATAAACAACCCTAACCAACCCCAAGCCCTCCACGTGAGGGCCTTTTTATTACTCAAAAAAGGACAATAAGATGTTGAATGAACAAGAAAAACACGCGTTTTTAAATGGCAATTACGGTATTCCGTTAGTGGGA > 1/1129253‑1129739 | gATAACACAACAGATTATTGCGATAACGGTATGAGACAGCAAATCTCCACCGAATGGGAGGCTAAGGCCAAAGCCGAATGGATATTCCAGCGTTTCTTTATGGGTTGAGGTAGATTTCTTAAAAAGCAAAAGATGCAAGGCATTAATAGATAAAGCCAGATCAACTGCCGACGAAATCATTAGAGACCTAAAAAATTTAAAAGATACTATTTTGTCCGTTGATACAGACAAA‑GCGTTTC‑TAATAATTTTc > 1:835834/1‑250 (MQ=255) cacaACAGATTATTGCGATAACGGTATGAGTCAGCAAATCTCCACCGAATGGGAGGCTAAGGCCAAAGCCGAATGGATATTCCAGCGTTTCTTTATGGGTTGAGGTAGATTTCTTAAAAAGCAAAAGATGCAAGGCATTAATAGATAAAGCCAGATCAACTGCCGACGAAATCATTAGAGACCTAAAAAATTTAAAAGATACTATTTTGTCCGTTGATACAGACAAA‑GCGTTTC‑TAATAATTTTCCCACAg < 1:461499/251‑1 (MQ=255) aGATTATTGCGATAACGGTATGAGTCAGCAAATCTCCACCGAATGGGAGGCTAAGGCCAAAGCCGAATGGATATTCCAGCGTTTCTTTATGGGTTGAGGTAGATTTCTTAAAAAGCAAAAGATGCAAGGCATTAATAGATAAAGCCAGATCAACTGCCGACGAAATCATTAGAGACCTAAAAAATTTAAAAGATACTATTTTGTCCGTTGATACAGACAAA‑GCGTTTC‑TAATAATTTTCCcac < 1:343961/243‑1 (MQ=255) gATTATTGCGATAACGGTATGAGTCAGCAAATCTCCACCGAATGGGAGGCTAAGGCCAAAGCCGAATGGATATTCCAGCGTTTCTTTATGGGTTGAGGTAGATTTCTTAAAAAGCAAAAGATGCAAGGCATTAATAGATAAAGCCAGATCAACTGCCGACGAAATCATTAGAGACCTAAAAAATTTAAAAGATACTATTTTGTCCGTTGATACAGACAAA‑GCGTTTC‑TAATAATTTTc > 1:473200/1‑238 (MQ=255) attattGCGATAACGGTATGAGTCAGCAAATCTCCACCGAATGGGAGGCTAAGGCCAAAGCCGAATGGATATTCCAGCGTTTCTTTATGGGTTGAGGTAGATTTCTTAAAAAGCAAAAGATGCAAGGCATTAATAGATAAAGCCAGATCAACTGCCGACGAAATCATTAGAGACCTAAAAAATTTAAAAGATACTATTTTGTCCGTTGATACAGACAAA‑GCGTTTC‑TAATAATTTTCCCACAGTGGGAAt < 1:594704/250‑1 (MQ=255) cGGTATGAGTCAGCAAATCTCCACCGAATGGGAGGCTAAGGCCAAAGCCGAATGGATATTCCAGCGTTTCTTTATGGGTTGAGGTAGATTTCTTAAAAAGCAAAAGATGCAAGGCATTAATAGATAAAGCCAGATCAACTGCCGACGAAATCATTAGAGACCTAAAAAATTTAAAAGATACTATTTTGTCCGTTGATACAGACAAA‑GCGTTTC‑TAATAATTTTCCCACAGTGGGAATCGCAGCTAC‑TGGaa < 1:945806/251‑1 (MQ=255) aaTCTCCACCGAATGGGAGGCTAAGGCCAAAGCCGAATGGATATTCCAGCGTTTCTTTATGGGTTGAGGTAGATTTCTTAAAAAGCAAAAGATGCAAGGCATTAATAGATAAAGCCAGATCAACTGCCGACGAAATCATTAGAGACCTAAAAAATTTAAAAGATACTATTTTGTCCGTTGATACAGACAAA‑GCGTTTC‑TAATAATTTTCCCACAGTGGGAATCGCAGCTAC‑TGGAAAG‑TTTGCCGCCCCGt > 1:337690/1‑251 (MQ=255) aaTCTCCACCGAATGGGAGGCTAAGGCCAAAGCCGAATGGATATTCCAGCGTTTCTTTATGGGTTGAGGTAGATTTCTTAAAAAGCAAAAGATGCAAGGCATTAATAGATAAAGCCAGATCAACTGCCGACGAAATCATTAGAGACCTAAAAAATTTAAAAGATACTATTTTGTCCGTTGATACAGACAAA‑GCGTTTC‑TAATAATTTTCCCACAGTGGGAATCGCAGCTAC‑TGGAAAG‑TTTGCCGCCCCGt > 1:843348/1‑251 (MQ=255) tcCACCGAATGGGAGGCTAAGGCCAAAGCCGAATGGATATTCCAGCGTTTCTTTATGGGTTGAGGTAGATTTCTTAAAAAGCAAAAGATGCAAGGCATTAATAGATAAAGCCAGATCAACTGCCGACGAAATCATTAGAGACCTAAAAAATTTAAAAGATACTATTTTGTCCGTTGATACAGACAAA‑GCGTTTC‑TAATAATTTTCCCACAGTGGGAATCGCAGCTAC‑TGGAAAG‑TTTGCCGCCCCGTAAAg < 1:289222/251‑1 (MQ=255) tcCACCGAATGGGAGGCTAAGGCCAAAGCCGAATGGATATTCCAGCGTTTCTTTATGGGTTGAGGTAGATTTCTTAAAAAGCAAAAGATGCAAGGCATTAATAGATAAAGCCAGATCAACTGCCGACGAAATCATTAGAGACCTAAAAAATTTAAAAGATACTATTTTGTCCGTTGATACAGACAAA‑GCGTTTC‑TAATAATTTTCCCACAGTGGGAATCGCAGCTAC‑TGGAAAG‑TTTGCCGCCCCGTAAAg < 1:669424/251‑1 (MQ=255) gAATGGGAGGCTAAGGCCAAAGCCGAATGGATATTCCAGCGTTTCTTTATGGGTTGAGGTAGATTTCTTAAAAAGCAAAAGATGCAAGGCATTAATAGATAAAGCCAGATCAACTGCCGACGAAATCATTAGAGACCTAAAAAATTTAAAAGATACTATTTTGTCCGTTGATACAGACAAA‑GCGTTTC‑TAATAATTTTCCCACAGTGGGAAt < 1:110940/212‑1 (MQ=255) ggCTAAGGCCAAAGCCGAATGGATATTCCAGCGTTTCTTTATGGGTTGAGGTAGATTTCTTAAAAAGCAAAAGATGCAAGGCATTAATAGATAAAGCCAGATCAACTGCCGACGAAATCATTAGAGACCTAAAAAATTTAAAAGATACTATTTTGTCCGTTGATACAGACAAA‑GCGTTTC‑TAATAATTTTCCCACAGTGGGAATCGCAGCTAC‑TGGAAAg > 1:1082415/1‑220 (MQ=255) cTAAGGCCAAAGCCGAATGGATATTCCAGCGTTTCTTTATGGGTTGAGGTAGATTTCTTAAAAAGCAAAAGATGCAAGGCATTAATAGATAAAGCCAGATCAACTGCCGACGAAATCATTAGAGACCTAAAAAATTTAAAAGATACTATTTTGTCCGTTGATACAGACAAA‑GCGTTTC‑TAATAATTTTCCCACAGTGGGAATCGCAGCTAC‑TGGAAAG‑TTTGCCGCCCCGTAAAGCGGGACTACCTGCTAc < 1:496279/251‑1 (MQ=255) aaGGCCAAAGCCGAATGGATATTCCAGCGTTTCTTTATGGGTTGAGGTAGATTTCTTAAAAAGCAAAAGATGCAAGGCATTAATAGATAAAGCCAGATCAACTGCCGACGAAATCATTAGAGACCTAAAAAATTTAAAAGATACTATTTTGTCCGTTGATACAGACAAA‑GCGTTTC‑TAATAATTTTCCCACAGTGGGAATCGCAGCTAC‑TGGAAAG‑TTTGCCGCCCCGTAAAGCGGGACTACCTGCTACTc < 1:1082225/251‑1 (MQ=255) aaGGCCAAAGCCGAATGGATATTCCAGCGTTTCTTTATGGGTTGAGGTAGATTTCTTAAAAAGCAAAAGATGCAAGGCATTAATAGATAAAGCCAGATCAACTGCCGACGAAATCATTAGAGACCTAAAAAATTTAAAAGATACTATTTTGTCCGTTGATACAGACAAA‑GCGTTTC‑TAATAATTTTCCCACAGTGGGAATCGCAGCTAC‑TGGAAAG‑TTTGCCGCCCCGTAAAGCGGGACTACCTGCTACTc < 1:780162/251‑1 (MQ=255) ggCCAAAGCCGAATGGATATTCCAGCGTTTCTTTATGGGTTGAGGTAGATTTCTTAAAAAGCAAAAGATGCAAGGCATTAATAGATAAAGCCAGATCAACTGCCGACGAAATCATTAGAGACCTAAAAAATTTAAAAGATACTATTTTGTCCGTTGATACAGACAAA‑GCGTTTC‑TAATAATTTTCCCACAGTGGGAATCGCAGCTAC‑TGGAAAG‑TTTGCCGCCCCGTAAAGc > 1:734672/1‑232 (MQ=255) ggATATTCCAGCGTTTCTTTATGGGTTGAGGTAGATTTCTTAAAAAGCAAAAGATGCAAGGCATTAATAGATAAAGCCAGATCAACTGCCGACGAAATCGTTAGAGACCTAAAAAATTTAAAAGATACTATTTTGTCCGTTGATACAGACAAA‑GCGTTTC‑TAATAATTTTCCCACAGTGGGAATCGCAGCTAC‑TGGAAAG‑t < 1:301607/201‑1 (MQ=255) ccAGCGTTTCTTTATGGTTTGAGGTAGATTTCTAAAAAAGAAAAAGATGCAAGGCATTAATAGAAAAAGCCAGATCAACTGCCGACAAAATCATTAGAGACATAAAAAATTTAAAAGATACTATTTTGTCCGTTGATACAGACAAA‑GCTTTTC‑TAATATTTTCCCCACAGTGGGAACCGCAGCTAC‑GGAAAAG‑TTTGCCGCCCCTTAAAGCGGGACTCCCTGCTCCCCCCGCCGATGTATCTTATCTAGAc < 1:673164/251‑1 (MQ=17) ccAGCGTTTCTTTATGGGTTGAGGTAGATTTCTAAAAAAGCAAAAGATGAAAGGCATTAATAGAAAAAGCCAGATCAACTGCCGACAAAATCATTAGAGCCCAAAAAAATTAAAAAGATACTATTTTGTCCGTTGATACAGACAAA‑GCGTTTC‑TAATAATTTCCCCACAGTGGGAATCGCAGCTAC‑TGAAAAG‑TTTGCCGCCCCGTAAAGCGGGACTCCCTGCTACTCCCGCCGATGTATCTTATCTAGAc < 1:293746/251‑1 (MQ=17) tttttttATGGGTTGAGGTAGATTTCTAAAAAAGCAAAGGATGCAAGGCATTAATAGATAAAGCCAAATCACCTGCCGACGAAATCATAAGAGACCTAAAAAATTTAAAAGATACTATTTTGTCCGTTGATACAGACAAA‑GCGTTTC‑TAATAATTTCCCCACAGTGGAAATCGCAGCTAC‑TGGAAAG‑TTTGCCGCCCCGTAAAGCGGGACTCCCTGCTACTCCCGCCGATGTATCGTATCTAGACAAATAc < 1:713010/247‑1 (MQ=14) tttttttATGGGTTGAGGTAGATTTCTAAAAAAGCAAAGGATGAAAGGCATAAATAGAAAAAGCCAGATCAACTGCCGACGAAATCATTAGAGACCAAAAAAATTAAAAAGATACTATTTTGTCCGTTGATACAGACAAA‑GCGTTTC‑TAATATTTTCCCCACAGGGGGAATCGCAGCTAC‑TGGAAAG‑TTTGCCGCCCCGTAAGGCGGGACTACCTGCTACTCCCGCCGATGTATCGTATCTAGACAAATAc < 1:75865/247‑1 (MQ=14) tttCTTTATGGGTTGAGGTAGATTTCTTAAAAAGAAAAAGATGCAAGGCATTAATAGATAAAGCCAGATCAACTGCCGACGAAATCATTAGAGACCAAAAAAATTTAAAAGATACTATTTTGTCCGTTGATACAGACAAA‑GCGTTTC‑TAATAATTTCCCCACAGTGGGAATCGCAGCTAC‑TGGAAAG‑TTTGCCGCCCCGTAAAGCGGGACTCCCTGCTACTCCCGCCGATGTATCTTATCTAGACAAATAc < 1:585104/251‑1 (MQ=255) tttCTTTAGGGGTTGAGGTAGATTTCTAAAAAAGAAAAGGATGCAAGGAATAAATAAATAAAGCAAGATCACCTGCCAACAAAATCATTAGAACCCAAAAAAATTAAAAAGATACTATTTTGTCCGTTGATACAAACAAA‑GCGTTTC‑TAATAATTTCCCCACGGTGGAAACCGCAGCTAC‑TGAAAAG‑TTTGCCCCCCCGAAAAGCGGGACTACCTGCTACTCCCGCCGATGTATCGTATCTAGACAAATAc < 1:784762/251‑1 (MQ=14) tCTTTATGGGTTGAGGTAGATTTCTTAAAAAGCAAAAGATGAAAGGCATTAATAGATAAAGCCAGATCAACTGCCGACGAAATCATTAGAGACCTAAAAAATTTAAAAGATACTATTTTGTCCGTTGATACAGACAAA‑GCGTTTC‑TAATAATTTTCCCACAGTGGGAATCGCAGCTAC‑TGGAAAG‑TTTGCCGCCCCGTAAAGCGGGACTACCTGCTACTCCCGCCGATGTATCTTATCTAGACAAATACaa > 1:935422/1‑251 (MQ=255) ttaggggTTGAGGTAGATTTCTAAAAAAGAAAAGGATGCAAGGAATTAAAAAAAAAAGCAAGATCAACGGCCGACAAAATCATTAGAGCCCAAAAAAATTAAAAAGATACTATTTTGTCCGTTGATACAGAAAAA‑GCGTTTC‑AAATAATTTCCCCACAGGGGGAATCGCAGCTAC‑GGGAAAT‑TTTGCCGCCCCGAAAAGGGGGACTCCCTGCTCCTCCCGCCGATGTATCGTATCTAGACAAATACAAGcc < 1:304102/247‑1 (MQ=9) ttATGGGTTGAGGTAGATTTTTTAAAAAGAAAAAAATGCAAGGCATTAAAAAAAAAAGCCAAATCAACTGCCGACAAAATCATTAGAGACCAAAAAAATTAAAAAGATACTATTTTGTCCGTTGATACAGACAAA‑GCTTTTC‑TAATAATTTCCCCACAGTGGGAATCGCAGCTAC‑TGGAAAG‑TTTGCCGCCCCGTAAAGCGGGACTCCCTGCTACTCCCGCCGATGTATCTTATCTAGACAAATACAAGcc < 1:11981/251‑1 (MQ=17) ggTTGAGGTAGATTTCTTAAAAAGCAAAAGATGCAAGGCATTAATAGATAAAGCCAGATCAACTGCCGACGAAATCATTAGAGACCTAAAAAATTTAAAAGATACTATTTTGTCCGTTGATACAGACAAA‑GCGTTTC‑TAATAATTTTCCCACAGTGGGAATCGCAGCTAC‑TGGAAAG‑TTTGCCGCCCCGTAAAGCGGGACTACCTGCTACTCccgcc < 1:1007216/217‑1 (MQ=255) gtggaggtaattttCTAAAAAAGAAAAAAAGGCAAGGAATAAATAAAAAAAGCCAGATCAACTCCCGACGAAATCATTAGAGACCAAAAAAATTAAAAAGATACTATTTTGCCCGTTGATACAGACAAA‑GCGTTTC‑TAATAATTTCCCCACGGTGGGAATCGCAGCTAC‑TGAAAAG‑TTTGCCGCCCCGTAAAGCGGGATTCCCTCCTACTCCCGCCGATGTATCTTATCTAGACAAATACAAGCCAAAGa < 1:129388/239‑1 (MQ=14) tAGATTTCTAAAAAAGCAAAAGATGCAAGGCATTAAAAAAAAAAGCAAGATCACCTGCCGACGAAATCATTAGAGACCAAAAAAATTAAAAAGATACTATTTTGTCCGTTGATACAGACAAA‑GCGTTTC‑TAATAATTTCCCCACAGTGGGAATCGCAGCTAC‑TGGAAAG‑TTTGCCCCCCCGTAAAGCGGGACTACCTGCTCCTCCCGCCGATGTATCTTATCTAGACAAATACAAGCCAAAGAAATAaac < 1:245463/250‑1 (MQ=17) tAGATTTCTAAAAAACCAAAGGATGAAAGGAATTAATAAATAAAGCCATATAAACTGCCGACGAAATCATTAAAGACCTAAAAAATTAAAAAAATCCTATTTTTTCCGTTGATACAAACAAG‑GCGTTTT‑TAATAATTTCCCCAGAGGGGGAATCGCAGCTCC‑TGAAAAG‑TTTGCCGCCCCGTAAAGCGGTACTACCTGCTCCTCCCGCCGATGTATCGTATCTAGACAAATACAAGCCAAAGAAATAaac < 1:934175/250‑1 (MQ=255) aTTTCTTAAAAAGCAAAGGATGCAAGGCATTAATAGATAAAGCCAGATCAACTGCCGACGAAATCATTAGAGACCTAAAAAATTTAAAAGATACTATTTTGTCCGTTGATACAGACAAA‑GCGTTTC‑TAATAATTTTCCCACAGTGGGAATCGCAGCTAC‑TGGAAAG‑TTTGCCGCCCCGTAAAGCGGGACTACCTGCTACTCCCGCCGATGTATCGTATCTAGACAAATACAAGCCAAAGAAATAaacaac > 1:301145/1‑250 (MQ=255) aaaaaaGCAAAAGATGCAAGGAATTAATAGAAAAGGCCGGTTCACCGGCCGACAAAATCATAAGAGCCCTAAAAAATTTAAAAGATACTATTTGGTCCGTTAATACAGACAAT‑GCGTTTC‑TAATAATTTCCCCCCAGTGGGAATCGCAGCTAC‑TGGAAAG‑TTTGCCCCCCCGTAAAGCGGGACTACCTGCTACTCCCGCCGATGTATCTTATCTAGACAAATACAAGCCAAAGAAATAAACAACCCTaacc < 1:190760/250‑1 (MQ=17) cATTAATAGAAAAAGCCAGATCACCTGCCGACAAAATCATTAGAGACCTAAAAAATTTAAAAGATACTATTTTGTCCGTTGATACAGACAAA‑GCTTTTC‑TAATAATTTCCCCACGGTGGGAATCGCAGCTAC‑TGGAAAG‑TTTGCCGCCCCGTAAAGCGGGACTACCTGCTACTCCCGCCGATGTATCTTATCTAGACAAATACAAGCCAAAGAAATAAACAACCCTAACCAACCCCAAGCCCTCCACGTg < 1:766820/250‑1 (MQ=255) cATTAATAGAAAAAGCCAGATCAACTGCCGACAAAATCATTAGAGACCAAAAAAATTTAAAAGATACTATTTTGTCCGTTGATACAGACAAA‑GCGTTTC‑TAATAATTTCCCCACAGTGGGAACCGCAGCTAC‑TGAAAAG‑TTTGCCGCCCCGTAAAGCGGGACTACCTGCTACTCCCGCCGATGTATCTTATCTAGACAAATACAAGCCAAAGAAATAAACAACCCTAACCAACCCCAAGCCCTCCACGTg < 1:181788/250‑1 (MQ=255) aTCATTAGAGACCTAAAAAATTTAAAAGATACTTTTTGGTCCGTTGATACAGACAAA‑GCGTTTC‑TAATAATTTCCCCACAGTGGGAATCGCAGCTAC‑TGGAAAG‑TTTGCCGCCCCGTAAAGCGGGACTCCCTGCTACTCCCGCCGATGTATCTTATCTAGACAAATACAAGCCAAAGAAATAAACAACCCTAACCAACCCCAAGCCCTCCACGTGAGGGCCTTTTTATTACTCAAAAAAGGACAATAAGAt < 1:160205/251‑1 (MQ=255) tCATTAGAGACCTAAAAAATTTAAAAGATACTATTTTGTCCGTTGATACAGACAAA‑GCGTTTC‑TAATAATTTTCCCACAGTGGGAATCGCAGCTAC‑TGGAAAG‑GTTGCCGCCCCGTAAAGCGGGACTACCTGCTACTCCCGCCGATGTATCTTATCTAGACAAATACAAGCCCAAGAAATAAACAACCCTAACCAACCCCAAGCCCTCCACGTGAGGGCCTTTTTATTACTCAAAAAAGAACAATAAGATg > 1:30110/1‑251 (MQ=255) ttAGAGACCTAAAAAATTTAAAAGATACTATTTTGTCCGTTGATACAGACAAA‑GCGTTTC‑TAATAATTTTCCCACAGTGGGAATCGCAGCTAC‑TGGAAAG‑TTTGCCGCCCCGTAAAGCGGGACTACCTGCTACTCCCGCCGATGTATCTTATCTAGACAAATACAAGCCAAAGAAATAAACAACCCTAACCAACCCCAAGCCCTCCACGTGAGGGCCTTTTTATTACTCAAAAAAGGACAATAAGATGt > 1:523997/1‑249 (MQ=255) agaCCGAAAAAATTTAAAAGATACTATTTTGCCCGTAAAT‑CAAAAAAACGCTTTTC‑TAATATTTTCCCCACGGTGGGAACCGCAGGTGC‑TGGAAAT‑TTTGCCCCCCAATAAGGGGGCCCCCCCTTCTACTCCCGCCGATGTATCTTATCTAGACAAATACAAGCCAAAGAAATAAACAACCCTAACCAACCCCAAGCCCTCCACGTGAGGGCCTTTTTATTTCTCAAAAAAGGACAATAAGATGTtgaa < 1:638167/249‑1 (MQ=12) aacaaaaaaaaTTTAAAAGATACTATTTTGTCCGTTGATACAGACAAA‑GCGTTTC‑TAATAATTTCCCCACAGGGGAAATCGCAGCTAC‑TGGAAAT‑TTTGCCGCCCCGGAAAGCGGGACTACCTGCTACTCCCGCCGATGTATCTTATCTAGACAAATACAAGCCAAAGAAATAAACAACCCTAACCAACCCCAAGCCCTCCACGTGAGGGCCTTTTTATTACTCAAAAAAGGACAATAAGATGTtgaatga < 1:1015362/246‑1 (MQ=255) aacaaaaaaaaTTAAAAAGATACTATTTTGTCCGTTGATACAGAAAAA‑GCGTTTC‑AAATAATTTCCCCACAGTGGGAACCGCAGCTAC‑TGGAAAT‑TTTCCCGCCCCGTAAAGCGGGACTCCCTGCTACTCCCGCCGATGTATCTTATCTAGACAAATACAAGCCAAAGAAATAAACAACCCTAACCAACCCCAAGCCCTCCACGTGAGGGCCTTTTTATTACTCAAAAAAGGACAATAAGATGTtgaatga < 1:496985/246‑1 (MQ=25) aaCCTAAAAAATTTAAAAGATACTATTTTGTCCGTTGATACAGACAAA‑GCTTTTC‑TAATAATTTCCCCACAGTGGAAATCGCAGCTAC‑TGAAAAG‑TTTGCCCCCCCGTAAGGCGGGACTCCCTGCTACTCCCGCCGATGTATCTTATCTAGACAAATACAAGCCAAAGAAATAAACAACCCTAACCAACCCCAAGCCCTCCACGTGAGGGCCTTTTTATTACTCAAAAAAGGACAATAAGATGTtgaatga < 1:483243/250‑1 (MQ=25) ccaaaaaaaTTAAAAAAATACTATTTGGTCCGTTGATACAGAAAAA‑GCGTTTC‑TAATAATTTCCCCACGGGGGGAACCGCAGCTAC‑TGGAAAG‑TTTGCCGCCCCGAAAAGCGGGACTCCCTGCTACTCCCGCCGATGTATCTTATCTAGACAAATACAAGCCAAAGAAATAAACAACCCTAACCAACCCCAAGCCCTCCACGTGAGGGCCTTTTTATTACTCAAAAAAGGACAATAAGATGTTGAATGAAc < 1:681848/248‑1 (MQ=25) aaaaaaaaTTAAAAAGATACTATTTTGTCAGTTAAAACAAACAAA‑GGGTTTA‑AAATAATTTTCCCACAGTGGGAACCGCAGC‑ACATGAAAAT‑TTCGCCGCCCCAAAAAGGGGAATTCCCTACTACCCCCGCCGATGTATCGTATCTAGACAAATACAAGCCAAAGAAATAAACAACCCTAACCAACCCCAAGCCCTCCACGTGAGGGCCTTTTTATTACTCAAAAAAGGACAATAAAATGTTGAATGAACa < 1:1018974/249‑1 (MQ=9) aGATACTATTTTGTCCGTTGATACAGACAAA‑GCGTTTC‑TAATAATTTTCCCACAGTGGGAATCGCAGCTAC‑TGGAAAG‑TTTGCCGCCCCGTAAAGCGGGACTACCTGCTACTCCCGCCGATGTATCTTATCTAGACAAATACAAGCCAAAGAAATAAACAACCCTAACCAACCCCAAGCCCTCCACGTGAGGGCCTTTTTATTACTCAAAAAAGGACAATAAGATGTtgaatga > 1:622380/1‑234 (MQ=255) aGATACTATTTTGTCCGTTGATACAGACAAA‑GCGTTTC‑TAATAATTTTCCCACAGTGGGAATCGCAGCTAC‑TGGAAAG‑TTTGCCGCCCCGTAAAGCGGGACTACCTGCTACTCCCGCCGATGTATCTTATCTAGACAAATACAAGCCAAAGAAATAAACAACCCTAACCAACCCCAAGCCCTCCACGTGAGGGCCTTTTTATTACTCAAAAAAGGACAATAAGATGTTGAATGAACAAGAAAAACACGCGt > 1:466427/1‑251 (MQ=255) gATACTATTTTGTCCGTTGATACAGACAAA‑GCGTTTC‑TAATAATTTTCCCACAGTGGGAATCGCAGCTAC‑TGGAAAG‑TTTGCCGCCCCGTAAAGCGGGACTACCTGCTACTCCCGCCGATGTATCTTATCTAGACAAATACAAGCCAAAGAAATAaac > 1:446401/1‑158 (MQ=255) atccTATTTTGTCCGTTGATACAGAAAAA‑GCGTTTC‑TAATAATTTTCCCACAGTGGAAATCGCAGCTAC‑GGAAAAT‑TTTGCCGCCCCGAAAAGGGGGACTACCTCCTACTCCCGCCGATGTATCTTATCTAGACAAATACAAGCCAAAGAAATAAACAACCCTAACCAACCCCAAGCCCTCCACGTGAg < 1:857118/186‑1 (MQ=21) aTACTATTTTGTCCGTTGATACAGACAAA‑GCGTTTC‑TAATAATTTTCCCACAGTGGGAATCGCAGCTAC‑TGGAAAG‑TTTGCCGCCCCGTAAAGCGGGACTACCTGCTACTCCCGCCGATGTATCTTATCTAGACAAATACAAGCCAAAGAAATAAACAACCCTAACCAACCCCAAGCCCTCCACGTg > 1:633305/1‑187 (MQ=255) aTACTATTTTGTCCGTTGATACAGACAAA‑GCGTTTC‑TAATAATTTTCCCACAGTGGGAATCGCAGCTAC‑TGGAAAG‑TTTGCCGCCCCGTAAAGCGGGACTACCTGCTACTCCCGCCGATGTATCTTATCTAGACAAATACAAGCCAAAGAAATAAACAACCCTAACCAACCCCAAGCCCTCCACGTGAGGGCCTTTTTATTACTCAAAAAAGGACAATAAGATGTTGAATGAACAAGAAAAACACGCGttt > 1:463762/1‑251 (MQ=255) tttATTTTGTCCTTTGATACAGACAAA‑‑CTTTTCTTAATATTTTCCCCACTGGGGAAATCGCAGCTAG‑T‑GAAAGATTTGCCGCCCCGTAAATCGGCTATACCGGCTCCTCCCGCCGATGTATCTTATCTAGACAAATACAAGCCAAAGAAATAAACAACCCTAACCAACCCCAAGCCCTCCACGTGAGGGCCTTTTTATTACTCAAAAAAGGACAATAAGATGTTGAATGAACAAGAAAAACACGCGttttt < 1:277291/249‑1 (MQ=17) cTATTTTGTCCGTTGATACAGACAAA‑GCGTTTC‑TAATAATTTTCCCACAGTGGGAATCGCAGCTAC‑TGGAAAG‑TTTGCCGCCCCGTAAAGCGGGACTACCTGCTACTCCCGCCGATGTATCTTATCTAGACAAATACAAGCCAAAGAAATAAACAACCCTAACCAAccc > 1:300653/1‑169 (MQ=255) tttttGTCCGTTGATACAGACAAG‑GCTTTTC‑TAATAATTTCCCCACGGGGGGAACCGCAGCTAC‑TGGAAAT‑TTTGCCGCCCCGAAAAGCGGGACTCCCTGCTACTCCCGCCGATGTATCTTATCTAGACAAATACAAGCCAAAGAAATAAACAACCCTAACCAACCCCAAGc < 1:888010/171‑1 (MQ=37) aTTTTGTCCGTTGATACAGACAAA‑GCGTTTC‑TAATAATTTTCCCACAGTGGGAATCGCAGCTAC‑TGGAAAG‑TTTGCCGCCCCGTAAAGCGGGACTACCTGCTACTCCCGCCGATGTATCTTATCTAGACAAATACAAGCCAAAGAAATAAACAACCCTAACCAACCCCAAGCCCTCCACGTGAGGGCCTTTTTATTACTCAAAAAAGGACAATAAGATGTTGAATGAACAAGAAAAACACGCGTTTTTaaa > 1:883700/1‑251 (MQ=255) gATACAGACAAA‑GCGTTTC‑TAATAATTTTCCCACAGTGGGAATCGCAGCTAC‑TGGAAAG‑TTTGCCGCCCCGTAAAGCGGGACTACCTGCTACTCCCGCCGATGTATCTTATCTAGACAAATACAAGCCAAAGAAATAAACAACCCTAACCAACCCCAAGCCCTCCACGTGAGGGCCTTTTTATTACTCAAAAAAGGACAATAAGATGTTGAATGAACAAGAAAAACACGCGTTTTTAAATGGCAATTACgg > 1:1029650/1‑251 (MQ=255) aaTACAGACAAA‑GCGTTTC‑TAATATTTTCCCCACAGTGGAAATCGCAGCTAC‑GGGAAAG‑TTTCCCCCCCCGTAAAGGGGGACTCCCTGCTCCCCCCGCCGATGTATCTTATCTAGACAAATACAAGCCAAAGAAATAAACAACCCTAACCAACCCCAAGCCCTCCACGTGAGGGCCTTTTTATTACTCAAAAAAGGACAATAAGATg < 1:563388/206‑1 (MQ=21) gACAAA‑GCGTTTC‑TAATAATTTTCCCACAGTGGGAATCGCAGCTAC‑TGGAAAG‑TTTGCCGCCCCGTAAAGCGGGACTACCTGCTACTCCCGCCGATGTATCTTATCTAGACAAATACAAGCCAAAGAAATAAACAACCCTAACCAACCCCAAGCCCTCCACGTGAGGGCCTTTTTATTACTCAAAAAAGGACAATAAGATGTTGAATGAACAAGAAAAACACGCGTTTTTAAATGGCAATTACGGTATTc > 1:136628/1‑250 (MQ=255) gCGTTTC‑TAATAATTTTCCCACAGTGGGAATCGCAGCTAC‑TGGAAAG‑TTTGCCGCCCCGTAAAGCGGGACTACCTGCTACTCCCGCCGATGTATCTTATCTAGACAAATACAAGCCAAAGAAATAAACAACCCTAACCAACCCCAAGCCCTCCACGTGAGGGCCTTTTTATTACTCAAAAAAGGACAATAAGATGTTGAATGAACAAGAAAAACACGCGTTTTTAAATGGCAATTACgg > 1:87255/1‑239 (MQ=255) gTTTC‑TAATAATTTTCCCACAGTGGGAATCGCAGCTAC‑TGGAAAG‑TTTGCCGCCCCGTAAAGCGGGACTACCTGCTACTCCCGCCGATGTATCTTATCTAGACAAATACAAGCCAAAGAAATAAACAACCCTAACCAACCCCAAGCCCTCCACGTGAGGGCCTTTTTATTACTCAAAAAAGGACAATAAGATGTTGAATGAACAAGAAAAACACGCGTTTTTAAATGGCAATTACGGTATTCCGTTAGTgg > 1:496686/1‑251 (MQ=255) tttC‑TAATAATTTTCCCCCGGGGGGAATCGCAGCTCC‑TGAAAAG‑TTTGCCGCCCCGAAAAGCGGGACTCCCTGCTACCCCCGCCGATGTATCTTATCTAGACAAATACAAGCCAAAGAAATAAACAACCCTAACCAACCCCAAGCCCTCCACGTGAGGGCCTTTTTATTACTCAAAAAAGGACAATAAGATGTTGAATGAACAAGAAAAACACGCGTTTTTAAATGGCAATTACGGTATTCCGTTAGTggg < 1:658514/251‑1 (MQ=18) tC‑TAATAATTTTCCCACAGTGGGAATCGCAGCTAC‑TGGAAAG‑TTTGCCGCCCCGTAAAGCGGGACTACCTGCTACTCCCGCCGATGTATCTTATCTAGACAAATACAAGCCAAAGAAATAAACAACCCTAACCAACCCCAAGCCCTCCACGTGAGGGCCTTTTTATTACTCAAAAAAGGACAATAAGATGTTGAATGAACAAGAAAAACACGCGTTTTTAAATGGCAATTACGGTATTCCGTTAGTGGGa > 1:295124/1‑250 (MQ=255) ttCCCACAGTGGGAATCGCAGCTAC‑TGGAAAG‑TTTGCCGCCCCGTAAAGCGGGACTACCTGCTACTCCCGCCGATGTATCTTATCTAGACAAATACAAGCCAAAGAAATAAACAACCCTAACCAACCCCAAGCCCTCCACGTGAGGGCCTTTTTATTACTCAAAAAAGGACAATAAGAt > 1:71486/1‑179 (MQ=255) | GATAACACAACAGATTATTGCGATAACGGTATGAGTCAGCAAATCTCCACCGAATGGGAGGCTAAGGCCAAAGCCGAATGGATATTCCAGCGTTTCTTTATGGGTTGAGGTAGATTTCTTAAAAAGCAAAAGATGCAAGGCATTAATAGATAAAGCCAGATCAACTGCCGACGAAATCATTAGAGACCTAAAAAATTTAAAAGATACTATTTTGTCCGTTGATACAGACAAA‑GCGTTTC‑TAATAATTTTCCCACAGTGGGAATCGCAGCTAC‑TGGAAAG‑TTTGCCGCCCCGTAAAGCGGGACTACCTGCTACTCCCGCCGATGTATCTTATCTAGACAAATACAAGCCAAAGAAATAAACAACCCTAACCAACCCCAAGCCCTCCACGTGAGGGCCTTTTTATTACTCAAAAAAGGACAATAAGATGTTGAATGAACAAGAAAAACACGCGTTTTTAAATGGCAATTACGGTATTCCGTTAGTGGGA > 1/1129253‑1129739 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |