Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	894,165	C→A	intergenic (‑11894/‑60619)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	894,165	0	C	A	100.0%	103.0 / NA	26	intergenic (‑11894/‑60619)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (9/17);  total (9/17)

CTGCAACGATTAACACGGCAACAGCAATAGCGATCAAACCATATTCAATTGCAGTCACACCTGCTTGATTTTCTCTGAAAGAACGGATTGCTTCAGCTGCTTTGATGTATGCTTTAGTAGTTAAAGTATTTAACATTGTCTTAATGCTCCTTTGTTGTTTATAGAAAATAAAATTCAAGAATCCTTGAGTTTGAACTTAGTGTCATTTATTTAATAAGCTTGTGGAATTAAATAAACGACCGGTCTTATAGTCCTGAAAAATTAAAATGCAAGTTTTTTTAGAATAATAAATTCACTATGTATATGTACGTATTGTGATTGCGAAAACTTTTTAATTAACAGTATGTTAAGGGTTATTGTAAATGAGCTATTTCTACTTTCTCTATTGATTTTACAGTAAATTTACAACTTTTAACTTTAATTACAAAAACACTTAAATATGTG  >  1/893921‑894364                                                                                                                                                                                                                                                     |                                                                                                                                                                                                        cTGCAACGATTAACACGGCAACAGCAATAGCGATCAAACCATATTCAATTGCAGTCACACCTGCTTGATTTTCTCTGAAAGAACGGATTGCTTCAGCTGCTTTGATGTATGCTTTAGTAGTTAAAGTATTTAACATTGTCTTAATGCTCCTTTGTTGTTTATAGAAAATAAAATTCAAGAATCCTTGAGTTTGAACTTAGTGTCATTTATTTAATAAGCTTGTGGAATTAAATAAACGACCGGTATtacag                                                                                                                                                                                                   <  1:1045841/251‑4 (MQ=255) cTGCAACGATTAACACGGCAACAGCAATAGCGATCAAACCATATTCAATTGCAGTCACACCTGCTTGATTTTCTCTGAAAGAACGGATTGCTTCAGCTGCTTTGATGTATGCTTTAGTAGTTAAAGTATTTAACATTGTCTTAATGCTCCTTTGTTGTTTATAGAAAATAAAATTCAAGAATCCTTGAGTTTGAACTTAGTGTCATTTATTTAATAAGCTTGTGGAATTAAATAAACGACCGGTATTATAg                                                                                                                                                                                                   <  1:986/251‑1 (MQ=255) cTGCAACGATTAACACGGCAACAGCAATAGCGATCAAACCATATTCAATTGCAGTCACACCTGCTTGATTTTCTCTGAAAGAACGGATTGCTTCAGCTGCTTTGATGTATGCTTTAGTAGTTAAAGTATTTAACATTGTCTTAATGCTCCTTTGTTGTTTATAGAAAATAAAATTCAAGAATCCTTGAGTTTGAACTTAGTGTCATTTATTTAATAAGCTTGTGGAATTAAATAAACGACCGGTATTATAg                                                                                                                                                                                                   <  1:818398/251‑1 (MQ=255) cTGCAACGATTAACACGGCAACAGCAATAGCGATCAAACCATATTCAATTGCAGTCACACCTGCTTGATTTTCTCTGAAAGAACGGATTGCTTCAGCTGCTTTGATGTATGCTTTAGTAGTTAAAGTATTTAACATTGTCTTAATGCTCCTTTGTTGTTTATAGAAAATAAAATTCAAGAATCCTTGAGTTTGAACTTAGTGTCATTTATTTAATAAGCTTGTGGAATTAAATAAACGACCGGTATTATAg                                                                                                                                                                                                   <  1:283743/251‑1 (MQ=255) cTGCAACGATTAACACGGCAACAGCAATAGCGATCAAACCATATTCAATTGCAGTCACACCTGCTTGATTTTCTCTGAAAGAACGGATTGCTTCAGCTGCTTTGATGTATGCTTTAGTAGTTAAAGTATTTAACATTGTCTTAATGCTCCTTTGTTGTTTATAGAAAATAAAATTCAAGAATCCTTGAGTTTGAACTTAGTGTCATTTATTTAATAAGCTTGTGGAATTAAATAAACGACCGGTATTATAg                                                                                                                                                                                                   <  1:118920/251‑1 (MQ=255)                      cAGCAATAGCGATCAAACCATATTCAATTGCAGTCACACCTGCTTGATTTTCTCTGAAAGAACGGATTGCTTCAGCTGCTTTGATGTATGCTTTAGTAGTTAAAGTATTTAACATTGTCTTAATGCTCCTTTGTTGTTTATAGAAAATAAAATTCAAGAATCCTTGAGTTTGAACTTAGTGTCATTTATTTAATAAGCTTGTGGAATTAAATAAACGACCGGTATTATAg                                                                                                                                                                                                   <  1:213448/230‑1 (MQ=255)                                           atTCAATTGCAGTCACACCTGCTTGATTTTCTCTGAAAGAACGGATTGCTTCAGCTGCTTTGATGTATGCTTTAGTAGTTAAAGTATTTAACATTGTCTTAATGCTCCTTTGTTGTTTATAGAAAATAAAATTCAAGAATCCTTGAGTTTGAACTTAGTGTCATTTATTTAATAAGCTTGTGGAATTAAATAAACGACCGGTATTATAGTCCTGAAAAATTAAAATg                                                                                                                                                                                 >  1:187302/1‑227 (MQ=255)                                                     aGTCACACCTGCTTGATTTTCTCTGAAAGAACGGATTGCTTCAGCTGCTTTGATGTATGCTTTAGTAGTTAAAGTATTTAACATTGTCTTAATGCTCCTTTGTTGTTTATAGAAAATAAAATTCAAGAATCCTTGAGTTTGAACTTAGTGTCATTTATTTAATAAGCTTGTGGAATTAAATAAACGACCGGTATTATAg                                                                                                                                                                                                   >  1:614671/1‑199 (MQ=255)                                                      gTCACACCTGCTTGATTTTCTCTGAAAGAACGGATTGCTTCAGCTGCTTTGATGTATGCTTTAGTAGTTAAAGTATTTAACATTGTCTTAATGCTCCTTTGTTGTTTATAGAAAATAAAATTCAAGAATCCTTGAGTTTGAACTTAGTGTCATTTATTTAATAAGCTTGTGGAATTAAATAAACGACCGGTATTATAGTCCTGAAAAATTAAAATGCAAGTTTTTTTAGAATAATAAATTCACtatgtata                                                                                                                                              >  1:246391/1‑251 (MQ=255)                                                                                           cTTCAGCTGCTTTGATGTATGCTTTAGTAGTTAAAGTATTTAACATTGTCTTAATGCTCCTTTGTTGTTTATAGAAAATAAAATTCAAGAATCCTTGAGTTTGAACTTAGTGTCATTTATTTAATAAGCTTGTGGAATTAAATAAACGACCGGTATTATAg                                                                                                                                                                                                   >  1:403423/1‑161 (MQ=255)                                                                                           cTTCAGCTGCTTTGATGTATGCTTTAGTAGTTAAAGTATTTAACATTGTCTTAATGCTCCTTTGTTGTTTATAGAAAATAAAATTCAAGAATCCTTGAGTTTGAACTTAGTGTCATTTATTTAATAAGCTTGTGGAATTAAATAAACGACCGGTATTATAGTCCTGAAAAATTAAAATGCAAGTTTTTTTAGAATAATAAATTCACTATGTATATGTACGTATtgtg                                                                                                                                 <  1:144675/227‑1 (MQ=255)                                                                                                   gctTTGATGTATGCTTTAGTAGTTAAAGTATTTAACATTGTCTTAATGCTCCTTTGTTGTTTATAGAAAATAAAATTCAAGAATCCTTGAGTTTGAACTTAGTGTCATTTATTTAATAAGCTTGTGGAATTAAATAAACGACCGGTATTATAg                                                                                                                                                                                                   <  1:554424/153‑1 (MQ=255)                                                                                                       tGATGTATGCTTTAGTAGTTAAAGTATTTAACATTGTCTTAATGCTCCTTTGTTGTTTATAGAAAATAAAATTCAAGAATCCTTGAGTTTGAACTTAGTGTCATTTATTTAATAAGCTTGTGGAATTAAATAAACGACCGGTATTATAGTCCTGAAAAATTAAAATGCAAGTTTTTTTAGAATAATAAATTCACTATGTATATGTACGTATTGTGATTGCGAAAACTTTTTAATTAACAGTATGTTAAggg                                                                                             <  1:597533/251‑1 (MQ=255)                                                                                                                                aTTTAACATTGTCTTAATGCTCCTTTGTTGTTTATAGAAAATAAAATTCAAGAATCCTTGAGTTTGAACTTAGTGTCATTTATTTAATAAGCTTGTGGAATTAAATAAACGACCGGtattat                                                                                                                                                                                                     >  1:61946/1‑122 (MQ=255)                                                                                                                                aTTTAACATTGTCTTAATGCTCCTTTGTTGTTTATAGAAAATAAAATTCAAGAATCCTTGAGTTTGAACTTAGTGTCATTTATTTAATAAGCTTGTGGAATTAAATAAACGACCGGTATTATAGTCCTGAAAAATTAAAATGCAAGTTTTTTTAGAATAATAAATTCACTATGTATATGTACGTATTGTGATTGCGAAAACTTTTTAATTAACAGTATGTTAAGGGTTATTGTAAATGAGCTATTTCTACt                                                                    <  1:416664/251‑1 (MQ=255)                                                                                                                                          gTCTTAATGCTCCTTTGTTGTTTATAGAAAATAAAATTCAAGAATCCTTGAGTTTGAACTTAGTGTCATTTATTTAATAAGCTTGTGGAATTAAATAAACGACCGGTATTATAGTCCTGAAAAATTAAAATGCAAGTTTTTTTAGAATAATAAATTc                                                                                                                                                        >  1:1050156/1‑157 (MQ=255)                                                                                                                                          gTCTTAATGCTCCTTTGTTGTTTATAGAAAATAAAATTCAAGAATCCTTGAGTTTGAACTTAGTGTCATTTATTTAATAAGCTTGTGGAATTAAATAAACGACCGGTATTATAGTCCTGAAAAATTAAAATGCAAGTTTTTTTAGAATAATAAATTCACtatgtata                                                                                                                                              >  1:223087/1‑167 (MQ=255)                                                                                                                                            cTTAATGCTCCTTTGTTGTTTATAGAAAATAAAATTCAAGAATCCTTGAGTTTGAACTTAGTGTCATTTATTTAATAAGCTTGTGGAATTAAATAAACGACCGGTATTATAg                                                                                                                                                                                                   <  1:381049/112‑1 (MQ=255)                                                                                                                                                    tCCTTTGTTGTTTATAGAAAATAAAATTCAAGAATCCTTGAGTTTGAACTTAGTGTCATTTATTTAATAAGCTTGTGGAATTAAATAAACGACCGGTATTATAGTCCTGAAAAATTAAAATGCAAGTTTTTTTAGAATAATAAATTCACTATGTATATGTACGTATTGTGATTGCGAAAACTTTTTAATTAACAGTATGTTAAGGGTTATTGTAAATGAGCTATTTCTACTTTCTCTATTGATTTTACAGt                                                <  1:748471/251‑1 (MQ=255)                                                                                                                                                     ccTTTGTTGTTTATAGAAAATAAAATTCAAGAATCCTTGAGTTTGAACTTAGTGTCATTTATTTAATAAGCTTGTGGAATTAAATAAACGACCGGTATTATAg                                                                                                                                                                                                   >  1:1190145/1‑103 (MQ=255)                                                                                                                                                     ccTTTGTTGTTTATAGAAAATAAAATTCAAGAATCCTTGAGTTTGAACTTAGTGTCATTTATTTAATAAGCTTGTGGAATTAAATAAACGACCGGTATTATAGTCCTGAAAAATTAAAATGCAAGTTTTTTTAGAATAATAAATTCACtatgtatatg                                                                                                                                            <  1:903238/158‑1 (MQ=255)                                                                                                                                                                                     aTCCTTGAGTTTGAACTTAGTGTCATTTATTTAATAAGCTTGTGGAATTAAATAAACGACCGGTATTATAg                                                                                                                                                                                                   <  1:225696/71‑1 (MQ=255)                                                                                                                                                                                            aGTTTGAACTTAGTGTCATTTATTTAATAAGCTTGTGGAATTAAATAAACGACCGGTATTATAg                                                                                                                                                                                                   >  1:118991/1‑64 (MQ=255)                                                                                                                                                                                              tttGAACTTAGTGTCATTTATTTAATAAGCTTGTGGAATTAAATAAACGACCGGTATTATAg                                                                                                                                                                                                   <  1:762664/62‑1 (MQ=255)                                                                                                                                                                                                  aaCTTAGTGTCATTTATTTAATAAGCTTGTGGAATTAAATAAACGACCGGTATTATAGTCCTGAAAAATTAAAATGCAAGTTTTTTTAGAATAATAAATTCACTATGTATATGTACGTATTGTGATTGCGAAAACTTTTTAATTAACAGTATGTTAAGGGTTATTGTAAATGAGCTATTTCTACTTTCTCTATTGATTTTACAGTAAATTTACAACTTTTAACTTTAATTACAAAAACACTTAAATAtgtg  <  1:425082/251‑1 (MQ=255)                                                                                                                                                                                                            cATTTATTTAATAAGCTTGTGGAATTAAATAAACGACCGGTATTATAGTCCTGAAAAATTAAAATGCAAGTTTTTTTAGAATAATAAATTCACTATGTATATGTACGTATTGTGATTGCGAAAACTTTTTAATTAACAGTATGTTAAGGGtt                                                                                           <  1:1031787/152‑1 (MQ=255)                                                                                                                                                                                                                                 gAATTAAATAAACGACCGGTATTATAGTCCTGAAAAATTAAAATGCAAGTTTTTTTAGAATAATAAATTCACTATGTATATGTACGTATTGTGATTGCGAAAACTTTTTAATTAACAGTATGTTAAGGGTTATTGTAAATGAGCTATTTCTACTTTCTCTATTGATTTTACAGTAAATTTACAACTTTTAACTTTAATTACAAAAACACTTAAATAtgtg  >  1:593798/1‑220 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                        CTGCAACGATTAACACGGCAACAGCAATAGCGATCAAACCATATTCAATTGCAGTCACACCTGCTTGATTTTCTCTGAAAGAACGGATTGCTTCAGCTGCTTTGATGTATGCTTTAGTAGTTAAAGTATTTAACATTGTCTTAATGCTCCTTTGTTGTTTATAGAAAATAAAATTCAAGAATCCTTGAGTTTGAACTTAGTGTCATTTATTTAATAAGCTTGTGGAATTAAATAAACGACCGGTCTTATAGTCCTGAAAAATTAAAATGCAAGTTTTTTTAGAATAATAAATTCACTATGTATATGTACGTATTGTGATTGCGAAAACTTTTTAATTAACAGTATGTTAAGGGTTATTGTAAATGAGCTATTTCTACTTTCTCTATTGATTTTACAGTAAATTTACAACTTTTAACTTTAATTACAAAAACACTTAAATATGTG  >  1/893921‑894364

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑