| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | 1 | 1,455,208 | 0 | A | C | 37.0% | 166.0 / 28.2 | 81 | intergenic (+148419/+105803) | unknown/unknown | tRNA‑Met‑CAT/tRNA‑Val‑GAC |
| Reads supporting (aligned to +/- strand): ref base A (37/14); new base C (0/30); total (37/44) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.28e-12 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CGAAACACAGCGCGAGCAATTGCGTCAGGTTCAAGCGCAATATGCACAATACAATCAAGTGTTGATTCAATTGCAAAGTTCTTTCAACAGT‑AAAAACCAAATGTTGCAGGAATTAATGCAGGAAATCGGCGAATTGGGT‑GTACGTGCCGACGAAGGCGCGGAAGAACGTGCAAAAATCCGTCGGGATGAGTTGTATCAACAACTTTCCACTAATCGTCAGTGCCGTTCTTACATTGAAAAACAACTCACCCTCATTGAAAGCGAAGCGGAAAA‑TCTTACCCGCCGCATTCGTAAAGCGGAACGGGACTACAAAACCCAGCGCGAATTGGTAACGGCGGCTAAAATGAGCTGGTGCGTGGTGCTGCGTTTATCTCGCAATTCTGATGTGGAAAAACGCTTAAATCGTCGTGAATTGGCATATTTATCCGCCGATGAGCTACGTTCCATGTCGGACAAAGCCCTTGGTGCGTTGCGTACGGCGGT > 1/1454961‑1455443 | cgAAACACAGCGCGAGCAATTGCGTCAGGTTCAAGCGCAATATGCACAATACAATCAAGTGTTGATTCAATTGCAAAGTTCTTTCAACAGT‑AAAAACCAAATGTTGCAGGAATTAATGCAGGAAATCGGCGAATTGGGT‑GTACGTGCCGACGAAGGCGCGGAAGAACGTGCAAAAATCCGTCGGGATGAGTTGTATCAACAACTTTCCACTAATCGTCAGTGCCGTTCTTACATTGAAAAACAACTCAcc < 1:972517/250‑1 (MQ=255) cgcgAGCAATTGCGTCAGGTTCAAGCGCAATATGCACAATACAATCAAGTGTTGATTCAATTGCAAAGTTCTTTCAACAGT‑AAAAACCAAATGTTGCAGGAATTAATGCAGGAAATCGGCGAATTGGGT‑GTACGTGCCGACGAAGGCGCGGAAGAACGTGCAAAAATCCGTCGGGATGAGTTGTATCAACAACTTTCCACTAATCGTCAGTGCCGTTCTTACATTGAAAAACAACTCACCCTCATTg < 1:34914/247‑1 (MQ=255) cgcgAGCAATTGCGTCAGGTTCAAGCGCAATATGCACAATACAATCAAGTGTTGATTCAATTGCAAAGTTCTTTCAACAGT‑AAAAACCAAATGTTGCAGGAATTAATGCAGGAAATCGGCGAATTGGGT‑GTACGTGCCGACGAAGGCGCGGAAGAACGTGCAAAAATCCGTCGGGATGAGTTGTATCAACAACTTTCCACTAATCGTCAGTGCCGTTCTTACATTGAAAAACAACTCACCCTCATTg < 1:965288/247‑1 (MQ=255) cgAGCAATTGCGTCAGGTTCAAGCGCAATATGCACAATACAATCAAGTGTTGATTCAATTGCAAAGTTCTTTCAACAGT‑AAAAACCAAATGTTGCAGGAATTAATGCAGGAAATCGGCGAATTGGGT‑GTACGTGCCGACGAAGGCGCGGAAGAACGTGCAAAAATCCGTCGGGATGAGTTGTATCAACAACTTTCCACTAATCGTCAGTGCCGTTCTTACATTGAAAAACAACTCACCCTCATTGAaagc < 1:10109/250‑1 (MQ=255) ggTTCAAGCGCAATATGCACAATACAATCAAGTGTTGATTCAATTGCAAAGTTCTTTCAACAGT‑AAAAACCAAATGTTGCAGGAATTAATGCAGGAAATCGGCGAATTGGGT‑GTACGTGCCGACGAAGGCGCGGAAGAACGTGCAAAAATCCGTCGGGATGAGTTGTATCAACAACTTTCCACTAATCGTCAGTGCCGTTCTTACATTGAAAAACAACTCACCCTCATTGAAAGCGAAGCGGAAAA‑TCTTa > 1:1186880/1‑251 (MQ=255) gTTCAAGCGCAATATGCACAATACAATCAAGTGTTGATTCAATTGCAAAGTTCTTTCAACAGT‑AAAAACCAAATGTTGCAGGAATTAATGCAGGAAATCGGCGAATTGGGT‑GTACGTGCCGACGAAGGCGCGGAAGAACGTGCAAAAATCCGTCGGGATGAGTTGTATCAACAACTTTCCACTAATCGTCAGTGCCGTTCTTACATTGAAAAACAACTCACCCTCATTGAAAGCGAAGCGGAAAA‑TCTTa > 1:145818/1‑250 (MQ=255) cAATATGCACAATACAATCAAGTGTTGATTCAATTGCAAAGTTCTTTCAACAGT‑AAAAACCAAATGTTGCAGGAATTAATGCAGGAAATCGGCGAATTGGGT‑GTACGTGCCGACGAAGGCGCGGAAGAACGTGCAAAAATCCGTCGGGATGAGTTGTATCAACAACTTTCCACTAATCGTCAGTGCCGTTCTTACATTGAAAAACAACTCACCCTCATTGAAAGCGAAGCGGAAAA‑TCTTACCCGCCGCAt > 1:100516/1‑251 (MQ=255) cAATATGCACAATACAATCAAGTGTTGATTCAATTGCAAAGTTCTTTCAACAGT‑AAAAACCAAATGTTGCAGGAATTAATGCAGGAAATCGGCGAATTGGGT‑GTACGTGCCGACGAAGGCGCGGAAGAACGTGCAAAAATCCGTCGGGATGAGTTGTATCAACAACTTTCCACTAATCGTCAGTGCCGTTCTTACATTGAAAAACAACTCACCCTCATTGAAAGCGAAGCGGAAAA‑TCTTACCCGCCGCAt > 1:265718/1‑251 (MQ=255) cAATATGCACAATACAATCAAGTGTTGATTCAATTGCAAAGTTCTTTCAACAGT‑AAAAACCAAATGTTGCAGGAATTAATGCAGGAAATCGGCGAATTGGGT‑GTACGTGCCGACGAAGGCGCGGAAGAACGTGCAAAAATCCGTCGGGATGAGTTGTATCAACAACTTTCCACTAATCGTCAGTGCCGTTCTTACATTGAAAAACAACTCACCCTCATTGAAAGCGAAGCGGAAAA‑TCTTACCCGCCGCAt > 1:190741/1‑251 (MQ=255) cAATATGCACAATACAATCAAGTGTTGATTCAATTGCAAAGTTCTTTCAACAGT‑AAAAACCAAATGTTGCAGGAATTAATGCAGGAAATCGGCGAATTGGGT‑GTACGTGCCGACGAAGGCGCGGAAGAACGTGCAAAAATCCGTCGGGATGAGTTGTATCAACAACTTTCCACTAATCGTCAGTGCCGTTCTTACATTGAAAAACAACTCACCCTCATTGAAAGCGAAGCGGAAAA‑TCTTACCCGCCGCAt > 1:931635/1‑251 (MQ=255) atGCACAATACAATCAAGTGTTGATTCAATTGCAAAGTTCTTTCAACAGT‑AAAAACCAAATGTTGCAGGAATTAATGCAGGAAATCGGCGAATTGGGT‑GTACGTGCCGACGAAGGCGCGGAAGAACGTGCAAAAATCCGTCGGGATGAGTTGTATCAACAACTTTCCACTAATCGTCAGTGCCGTTCTTACATTGAAAAACAACTCACCCTCATTGAAAGCGAAGCGGAAAA‑TCTTAcc < 1:615194/239‑1 (MQ=255) atGCACAATACAATCAAGTGTTGATTCAATTGCAAAGTTCTTTCAACAGT‑AAAAACCAAATGTTGCAGGAATTAATGCAGGAAATCGGCGAATTGGGT‑GTACGTGCCGACGAAGGCGCGGAAGAACGTGCAAAAATCCGTCGGGATGAGTTGTATCAACAACTTTCCACTAATCGTCAGTGCCGTTCTTACATTGAAAAACAACTCACCCTCATTGAAAGCGAAGCGGAAAA‑TCTTACCCGCCGCATTCGt > 1:472731/1‑251 (MQ=255) gCACAATACAATCAAGTGTTGATTCAATTGCAAAGTTCTTTCAACAGT‑AAAAACCAAATGTTGCAGGAATTAATGCAGGAAATCGGCGAATTGGGT‑GTACGTGCCGACGAAGGCGCGGAAGAACGTGCAAAAATCCGTCGGGATGAGTTGTATCAACAACTTTCCACTAATCGTCAGTGCCGTTCTTACATTGAAAAACAACTCACCCTCATTg > 1:399126/1‑214 (MQ=255) gCACAATACAATCAAGTGTTGATTCAATTGCAAAGTTCTTTCAACAGT‑AAAAACCAAATGTTGCAGGAATTAATGCAGGAAATCGGCGAATTGGGT‑GTACGTGCCGACGAAGGCGCGGAAGAACGTGCAAAAATCCGTCGGGATGAGTTGTATCAACAACTTTCCACTAATCGTCAGTGCCGTTCTTACATTGAAAAACAACTCACCCTCATTGAAAGCGAAGCGGAAAA‑TCTTACCCGCCGCATTCGTaa > 1:909997/1‑251 (MQ=255) gCACAATACAATCAAGTGTTGATTCAATTGCAAAGTTCTTTCAACAGT‑AAAAACCAAATGTTGCAGGAATTAATGCAGGAAATCGGCGAATTGGGT‑GTACGTGCCGACGAAGGCGCGGAAGAACGTGCAAAAATCCGTCGGGATGAGTTGTATCAACAACTTTCCACTAATCGTCAGTGCCGTTCTTACATTGAAAAACAACTCACCCTCATTGAAAGCGAAGCGGAAAA‑TCTTACCCGCCGCATTCGTaa > 1:1077621/1‑251 (MQ=255) cacaATACAATCAAGTGTTGATTCAATTGCAAATTTCTTTCAACAGT‑AAAAACCAAATGTTGCAGAAATTAATGCAGAAAATCGGCGAATTGGGT‑GTACGTGCCGACGAAGGCGCGAAAGAACGTGAAAAAATCCGTCGGGATGAGTTGTATCAACAACTTTCCACTAATCGTCAGTGCCGTTCTTACATTGAAAAACACCTCCCCCTAATTGAAAGCGAAGCGGAAAA‑TCTTACCCGCCGCATTCGTaa < 1:205082/250‑1 (MQ=255) caatacaatCAAGTGTTGATTCAATTGCAAAGTTCTTTCAACAGT‑AAAAACCAAATGTTGCAGGAATTAATGCAGGAAATCGGCGAATTGGGT‑GTACGTGCCGACGAAGGCGCGGAAGAACGTGCAAAAATCCGTCGGGATGAGTTGTATCAACAACTTTCCACTAATCGTCAGTGCCGTTCTTACATTGAAAAACAACTCACCCTCATTGAAAGCGAAGCGGAAAA‑TCTTAccc < 1:439637/235‑1 (MQ=255) caatacaatCAAGTGTTGATTCAATTGCAAAGTTCTTTCAACAGT‑AAAAACCAAATGTTGCAGGAATTAATGCAGGAAATCGGCGAATTGGGT‑GTACGTGCCGACGAAGGCGCGGAAGAACGTGCAAAAATCCGTCGGGATGAGTTGTATCAACAACTTTCCACTAATCGTCAGTGCCGTTCTTACATTGAAAAACAACTCACCCTCATTGAAAGCGAAGCGGAAAA‑TCTTACCCGCCGCATTCGTAAAg > 1:745859/1‑250 (MQ=255) caatacaatCAAGTGTTGATTCAATTGCAAAGTTCTTTCAACAGT‑AAAAACCAAATGTTGCAGGAATTAATGCAGGAAATCGGCGAATTGGGT‑GTACGTGCCGACGAAGGCGCGGAAGAACGTGCAAAAATCCGTCGGGATGAGTTGTATCAACAACTTTCCACTAATCGTCAGTGCCGTTCTTACATTGAAAAACAACTCACCCTCATTGAAAGCGAAGCGGAAAA‑TCTTACCCGCCGCATTCGTAAAg > 1:192459/1‑250 (MQ=255) caatacaatCAAGTGTTGATTCAATTGCAAAGTTCTTTCAACAGT‑AAAAACCAAATGTTGCAGGAATTAATGCAGGAAATCGGCGAATTGGGT‑GTACGTGCCGACGAAGGCGCGGAAGAACGTGCAAAAATCCGTCGGGATGAGTTGTATCAACAACTTTCCACTAATCGTCAGTGCCGTTCTTACATTGAAAAACAACTCACCCTCATTGAAAGCGAAGCGGAAAA‑TCTTACCCGCCGCATTCGTAAAg > 1:1155492/1‑250 (MQ=255) atacaatCAAGTGTTGATTCAATTGCAAAGTTCTTTCAACAGT‑AAAAACCAAATGTTGCAGGAATTAATGCAGGAAATCGGCGAATTGGGT‑GTACGTGCCGACGAAGGCGCGGAAGAACGTGCAAAAATCCGTCGGGATGAGTTGTATCAACAACTTTCCACTAATCGTCAGTGCCGTTCTTACATTGAAAAACAACTCACCCTCATTGAAAGCGAAGCGGAAAA‑TCTTACCCGCCGCATTCGTAAAGc > 1:1136437/1‑249 (MQ=255) atacaatCAAGTGTTGATTCAATTGCAAAGTTCTTTCAACAGT‑AAAAACCAAATGTTGCAGGAATTAATGCAGGAAATCGGCGAATTGGGT‑GTACGTGCCGACGAAGGCGCGGAAGAACGTGCAAAAATCCGTCGGGATGAGTTGTATCAACAACTTTCCACTAATCGTCAGTGCCGTTCTTACATTGAAAAACAACTCACCCTCATTGAAAGCGAAGCGGAAAA‑TCTTACCCGCCGCATTCGTAAAGCgg > 1:1090145/1‑251 (MQ=255) atacaatCAAGTGTTGATTCAATTGCAAAGTTCTTTCAACAGT‑AAAAACCAAATGTTGCAGGAATTAATGCAGGAAATCGGCGAATTGGGT‑GTACGTGCCGACGAAGGCGCGGAAGAACGTGCAAAAATCCGTCGGGATGAGTTGTATCAACAACTTTCCACTAATCGTCAGTGCCGTTCTTACATTGAAAAACAACTCACCCTCATTGAAAGCGAAGCGGAAAA‑TCTTACCCGCCGCATTCGTAAAGCgg > 1:539087/1‑251 (MQ=255) atCAAGTGTTGATTCAATTGCAAAGTTCTTTCAACAGT‑AAAAACCAAATGCTGCAGGAATTAATGCAGGAAATCGGCGAATTGGGT‑GTACGTGCCGACGAAGGCGCGGAAGAACGTGCAAAAATCCGTCGGGATGAGTTGTATCAACAACTTTCCACTAATCGTCAGTGCCGTTCTTACATTGAAAAACAACTCACCCTCATTGAAAGCGAAGCGGAAAA‑TCTTACCCGCCGCATTCGTAAAGCGGAACgg > 1:719632/1‑251 (MQ=255) aaGTGTTGATTCAATTGCAAAGTTCTTTCAACAGT‑AAAAACCAAATGTGGCAGGAATTAATGCAGGAAATCGGCGAATGGGGT‑GTACGTGCCGACGAAGGCGCGGAAGAACGTGAAAAAATCCGTCGGGATGAGTTGTATCAACAACTTTCCACTAATCGTCAGTGCCGTTCTTACATTGAAAAACAACTCCCCCTCATGGAAAGCGAAGCGGAAAA‑TCTTACCCGCCGCATTCGTAAAGCGGAACGGGAc < 1:1044186/251‑1 (MQ=255) aaGTGTTGATTCAATTGCAAAGTTCTTTCAACAGA‑AAAAACCAAATGTTGCAGGAATTAATGCAGGAAATCGGCGAATTGGGT‑GTACGTGCCGACGAAGGCGCGGAAGAACGTGAAAAAATCCGTCGGGATGAGTTGTATCAACAACTTTCCACTAATCGTCAGTGCCGTTCTTACATTAAAAAACAACTCCCCCTCATTGAAAGCGAAGCGAAAAA‑TCTTCCCCGCCGCATTCGTAAAGCGGAACGGGAc < 1:843225/251‑1 (MQ=255) aaGTGTTGATTCAATTGAAAAGTTCTTTCAACAGA‑AAAAACCAAATGTTGCAGGAATTAATGCAGAAAATCGGCGAATTGGGT‑GTACGTGCCGACGAAGGCGCGGAAGAACGTGCAAAAATCCGTCGGGATGAGTTGTATCAACAACTTTCCACTAATCGTCAGTGCCGTTCTTACATTGAAAAACAACTCCCCCTCATTAAAAGCGAAGCGGAAAA‑TCTTACCCGCCGCATTCGTAAAGCGGAACGGGAc < 1:109495/251‑1 (MQ=255) ggttttGATTCAATTGCAAAGTTCTTTCAACAGT‑AAAAACAAAATGTTGCAGGAATTAATGCAGAAAATCGGCGAATTGGGT‑GTCCGTGCCGACGAAGGCGCGGAAGACCGTGCAAAAATCCGTGGGGATGAGTTGTATCAACAATTTCCCACTAATCGTCAGTGCCGTTCTTACATTGAAAAACACCTCCCCCTCATTGAAAGCGAAGCGGAAAA‑TCTTACCCGCCGCATTCGTAAAGCGGAACGGGAc < 1:968260/246‑1 (MQ=255) ttGATTAAATTGCAAAGTTCTTAAAAC‑GTAAAAAACAAAATGTTGAAGGATTTATTGCAGGACATGGGTGACTTGGGT‑GTACTTGCGGATGATGGCGCGGAAGAACGTGAAAAAACCCTTGGGGAGTAATTGACTCAACAACTTTCCATAAATCCTCAGCGCCGTTCTTACTTAAAAAAAAAACACCCCCCAATTAAAAGCCAAGGGGAAAA‑TCTTACCCGCCGCATTCGTAAAGCGGAACGGGACTACaa < 1:474150/251‑1 (MQ=255) tCAATTGCAAAGTTCTTTCAACAGT‑AAAAACCAAATGTTGCAGGAATTAATGCAGGAAATCGGCGAATGGGGT‑GTACGTGCCGACGAAGGCGCGGAAGAACGTGCAAAAATCCGTCGGGATGAGTTTTATCAACAACTTTCCACTAATCGTCAGTGCCGTTCTTACATTAAAAAACAACCCCCCCCCATTAAAAGCGAAGCGGAAAA‑TCTTACCCGCCGCATTCGTAAAGCGGAACGGGACTACAAAAccc < 1:697219/251‑1 (MQ=255) tCAATTGAAAAGTTCTTTCAACAGA‑AAAAACCAAATGTTGCAGGAATTAATGCAGGAAATCGGCGAATTGGGT‑GTCCGTGCCGACGAAGGCGCGGAAGAACGTGCAAAAATCCGTCGGGATGAGTTGTATCAACAACTTTCCACTAATCGTCAGTGCCGTTCTTACATTGAAAAACAACTCACCCTCATTGAAAGCGAAGCGGAAAA‑TCTTACCCGCCGCATTCGTAAAGCGGAACGGGACTACAAAAccc < 1:591498/251‑1 (MQ=255) caaaTTGAAAAGTTTTTTCAACAGT‑AAAACCAAAATGTTGCAGAAATAAATGCGGGAAATCGGCGATTTGGGT‑GTACGTGCCGACGAAGGCGCGGAAGACCGTGCAAAAATCCGTCGGGATGATTTGTATCAACAACTTTCCCCTAATCGTCAGTGCCTTTCTTACATTGAAAAACAACTCCCCCTCATTGAAAGCGAAGCGGAAAA‑TCTTACCCGCCGCATTCGTAAAGCGGAACGGGACTACAAAAccc < 1:587183/249‑1 (MQ=255) aTTGCAAAGTTCTTTCAACAGT‑AAAAACCAAATGTTGCAGGAATTAATGCAGGAAATCGGCGAATTGGGT‑GTACGTGCCGACGAAGGCGCGGAAGAACGTGCAAAAATCCGTCGGGATGAGTTGTATCAACAACTTTCCACTAATCGTCAGTGCCGTTCTTACATTGAAAAACAACTCACCCTCATTGAAAGCGAAGCGGAAAA‑TCTTACCCGCCGCATTCGTAAAGCGGAACGGGACTACAAAACCCAg > 1:964642/1‑250 (MQ=255) tGCAAAGTTCTTTCAACAGT‑AAAAACCAAATGTTGCAGGAATTAATGCAGGAAATCGGCGAATTGGGT‑GTACGTGCCGACGAAGGCGCGGAAGAACGTGCAAAAATCCGTCGGGATGAGTTGTATCAACAACTTTCCACTAATCGTCAGTGCCGTTCTTACATTGAAAAACAACTCACCCTCATTGAAAGCGAAGCGGAAAA‑TCTTACCCGCCGCATTCGTAAAGCGGAACGGGACTACAAAACCCAgcg > 1:637808/1‑250 (MQ=255) ttCTTTCAACAGT‑AAAACCCAAATGTTGCAGGAATTAATGCAGGAACTCGGCGATTTGGGT‑GTACGTGCCGACGAAGGCGCGGAAAAACGTGCAAAAATCCGTCGGGATGAGTTGTATCAACAACTTTCCACTAACCGTCAGTGCCGTTCTTACAATGAAAAACAACTCCCCCTCATTGAAAGCGAAGCGGAAAA‑TCTTCCCCGCCGCATTCGTAAAGCGGAACGGGACTACAAAACCCAGCGCGAAtt < 1:939418/249‑1 (MQ=255) cTTTCAACAGT‑AAAAACAAAATGTTGCAGGAATTAATGCAGGAAATCGGCGAATTGGGT‑GTACGTGCCGACGAAGGCGCGGAAGAACGTGCAAAAATCCGTCGGGATGAGTTGTATCAACAACTTTCCACTAATCGTCAGTGCCGTTCTTACATTGAAAAACAACTCCCCCTCATTGAAAGCGAAGCGGAAAA‑TCTTCCCCGCCGCATTCGTAAAGCGGAACGGGACTACAAAAccc < 1:769052/237‑1 (MQ=255) gT‑AAAAACCAAATGTTGCAGGAATTAATGCAGGAAATCGGCGAATTGGGT‑GTACGTGCCGACGAAGGCGCGGAAGAACGTGCAAAAATCCGTCGGGATGAGTTGTATCAACAACTTTCCACTAATCGTCAGTGCCGTTCTTACATTGAAAAACAACTCACCCTCATTg > 1:996680/1‑168 (MQ=255) aaCCAAATGTTGCAGGAATTAATGCAGGAAATCGGCGAATTGGGT‑GTACGTGCCGACGAAGGCGCGGAAGAACGTGCAAAAATCCGTCGGGATGAGTTGTATCAACAACTTTCCACTAATCGTCAGTGCCGTTCTTACATTGAAAAACAACTCACCCTCATTGAAAGCGAAGCGGAAAA‑TCTTACCCGCCGCATTCGTAAAGCGGAACGGGACTACAAAACCCAGCGCGAATTGGTAACGGCGGCTAAAAt > 1:983510/1‑251 (MQ=255) ttGCAGGAATTAATGCAGGAAATCGGCGAATTGGGT‑GTACGTGCCGACGAAGGCGCGGAAGAACGTGCAAAAATCCGTCGGGATGAGTTGTATCAACAACTTTCCACTAATCGTCAGTGCCGTTCTTACATTGAAAAACAACTCACCCTCATTGAAAGCGAAGCGGAAAA‑t < 1:57897/171‑1 (MQ=255) ttGCAGGAATTAATGCAGGAAATCGGCGAATTGGGT‑GTACGTGCCGACGAAGGCGCGGAAGAACGTGCAAAAATCCGTCGGGATGAGTTGTATCAACAACTTTCCACTAATCGTCAGTGCCGTTCTTACATTGAAAAACAACTCACCCTCATTGAAAGCGAAGCGGAAAA‑TCTTACCCGCCGCATTCGTAAAGCGGAACGGGACTACAAAACCCAGCGCGAATTGGTAACGGCGGCTAAAATGAGCTGGTg > 1:345113/1‑251 (MQ=255) cAGGAATTAATGCAGGAAATCGGCGAATTGGGT‑GTGCGTGCCGACGAAGGCGGGGAAGAACGTGAAAAAATCCGTCGGGATGAGTTGTATCAACACCTTTCCACTAATCGTCAGTGCCGTTCTTACATTGAAAAACACCTCCCCCTCATTAAAAGCAAAGCGGAAAA‑TCTTACCCGCCGCATTCGTAAAGCGGAACGGGACTACAAAACCCAGCGCGAATTGGTAACGGCGGCTAAAATGAGCTGGTGCg < 1:935180/250‑1 (MQ=255) gAATTAATGCAGGAAATCGGCGAATTGGGT‑GTACGTGCCGACGAAGGCGCGGAAGAACGTGCAAAAATCCGTCGGGATGAGTTGTATCAACAACTTTCCACTAATCGTCAGTGCCGTTCTTACATTGAAAAACAACTCCCCCTCATTGAAAGCGAAGCGGAAAA‑TCTTACCCGCCGCATTCGTAAAGCGGAACGGGACTACaa < 1:457600/203‑1 (MQ=255) tgaaGGAAATGGGCGAATTGGGT‑GTACGTGCCGACGAAGGCGCGAAAGAACGTGCAAAAACCCGTCGGGATGAATTTTACAAAAAACTTTCCCCTAACCGGCAGTGCCGTTCTTAAATTAAAAAACAACCCCCCCTCATTAAAAGAGAAGGGAAAAA‑TCTTACCCGCCGCATTCGTAAAGCGGAACGGGACTACAAAACCCAGCGCGAATTGGTAACGGCGGCTAAAATGAGCTGGTGCGTGGTGCTGCGt < 1:1020345/248‑1 (MQ=255) tGCAGGAAATCGGCGAATTGGGT‑GTCCGTGCCGACGAAGGCGCGAAAGAACGTGAAAAAATCCGTCGGGATGTGTTGTATCAACAATTTTCCACTAATCGTCGGGGCCGTTCTTACATTGAAAAACACCTCCCCCTCATTGAAAGCGAAGCGGAAAA‑TCTTCCCCGCCGCATTCGTAAAGCGGAACGGGACTACAAAACCCAGCGCGAATTGGTAACGGCGGCTAAAATGAGCTGGTGCGTGGTGCTGCGt < 1:946720/251‑1 (MQ=255) gCAGGAAATCGGCG‑ATTGGGTGGTACGTGCCGACGAAGGCGCGGAAGACCGTGAAAAAACCCGTGGGGATGAGTTGTATCACCACCTTCCCACTAATCGTCAGTGCCGTTCTTACATTAAAAAACACCCCCCCCTCATTAAAGGCGAAGCGAAAAT‑TCTTCCCCGCCGCATTCGTAAAGCGGAACGGGACTACAAAACCCAGCGCGAATTGGTAACGGCGGCTAAAATGAGCTGGTGCGTGGTGCTGCGt < 1:444959/250‑1 (MQ=255) gCAGAAAACCGGCGAATGGGGT‑GTCCGTGCCGACGAAGGCGCGGAAGAACGTGCAAAAATCCGTCGGGATGAGTTGTATCAACAACTTTCCACAAATCGTCAGTGCCGTTCTTACATTAAAAAACAACTCCCCCTCATTAAAAGCGAAGCGAAAAA‑TCTTCCCCGCCGCATTCGTAAAGCGGAACGGGACTACAAAACCCAGCGCGAATTGGTAACGGCGGCTAAAATGAGCTGGTGCGTGGTGCTGCGt < 1:214893/250‑1 (MQ=255) aaGGAAATCGGCGAATTGGGT‑GTACGTGCCGACAAAGGCGCGAAAGAACGTGCAAAAACCCGTCGGGATGAGTGGTAACAACAACTTTCCACAAATCGTCAGTGCCTTTCTTACATTGAAAAACAACCCACCCTCATTGAAAGCGAAGCGAAAAA‑TCTTACCCGCCGCATTCGTAAAGCGGAACGGGACTACAAAACCCAGCGCGAATTGGTAACGGCGGCTAAAATGAGCTGGTGCGTGGTGCTGCGttt < 1:1087492/250‑1 (MQ=255) ggAAATCGGCGAATTGGGT‑GTACGTGCCGACGAAGGCGCGGAAGAACGTGCAAAAATCCGTCGGGATGAGTTGTATCAACAACTTTCCACTAATCGTCAGTGCCGTTCTTACATTGAAAAACAACTCACCCTCATTGAaag < 1:611683/141‑1 (MQ=255) gaaaagcGGCGAATTGGGT‑GTACGTGCCGACGAAGGCGCGGAAGAACGTGAAAAAATCCGTCGGGATGAGTTGTATCAACACCTTTCCACAAATCGTCAGTGCCGTTCTTACATTGAAAAACAACTCCCCCTCATTGAAAGCGAAGCGAAAAA‑TCTTACCCGCCGCATTCGTaa < 1:213764/168‑1 (MQ=255) aaTTGGGT‑GTACGTGCCGACGAAGGCGCGGAAGAACGTGCAAAAATCCGTCGGGATGAGTTGTATCAACAACTTTCCACTAATCGTCAGTGCCGTTCTTACATTGAAAAACAACTCACCCTCATTGAAAGCGAAGCGGAAAA‑TCTTACCCGCCGCATTCGTAAAGCGGAACGGGACTACAAAACCCAGCGCGAATTGGTAACGGCGGCTAAAATGAGCTGGTGCGTGGTGCTGCGTTTATCTCGCAAtt > 1:527183/1‑249 (MQ=255) tccGTGCCGACGAAGGCGCGAAAGAACGTGCAAAAATCCGTGGGGATGAGTTGTATCAACAACTTTCCACTAATCGTCAGTGCCGTTCTTACATTGAAAAACACCTCCCCCTCATGGAAAGCGAAGCGAAAAA‑TCTTACCCGCCGCATTCGTAAAGCGGAACGGGACTACAAAACCCAGCGCGAATTGGTAACGGCGGCTAAAATGAGCTGGTGCGTGGTGCTGCGTTTATCTCGCAATTCTGATGTGGaa < 1:1029244/249‑1 (MQ=255) ttcccggcgacggcgggaaagaaCGTGAAAAAATCCGTCGGGATGATTTGGTTCAACAACTTACCACTATTCGTAAGGGCCGTTATTCTATTGAAAACCAACTCCCTCTCTATGAAAGCGAACTGAAAAA‑TCTTCCCCGCCGCATTCGTAAAGCGGAACGGAACTACAAAACCCAGCGCGAATTGGTAACGGCGGCTAAGATGAGCTGGTGCGTGGTGCTGCGTTTATCTCGCAATTCTGATGTGGaaaaa < 1:767682/233‑1 (MQ=255) tGCCGACGAAGGCGCGGAAGAACGTGCAAAAACCCGTCGGGATGAGTTGTATCAACAACTTTCCACTAATCGTCAGTGCCGTTCTTACATTGAAAAACAACTCCCCCTCATTGAAAGCGAAGCGAAAAA‑TCTTCCCCGCCGCATTCGTAAAGCGGAACGGGACTACAAAACCCAGCGCGAATTGGTAACGGCGGCTAAAATGAGCTGGTGCGTGGTGCTGCGTTTATCTCGCAATTCTGATGTGGaaaaa < 1:822811/250‑1 (MQ=255) cgacgaAGGCGCGGAAGAACGTGCAAAAATCCGTCGGGATGAGTTGTATCAACAACTTTCCACTAATCGTCAGTGCCGTTCTTACATTGAAAAACAACTCACCCTCATTGAAAGCGAAGCGGAAAA‑TCTTACCCGCCGCATTCGTAAAGCGGAACGGGACTACAAAACCCAGCGCGAATTGGTAACGGCGGCTAAAATGAGCTGGTGCGTGGTGCTGCGTTTATCTCGCAATTCTGATGTGGAAAAACg > 1:589155/1‑249 (MQ=255) aaCGTGCAAAAATCCGTCGGGATGAGTTGTATCAACAACTTTCCACTAATCGTCAGTGCCGTTCTTACATTGAAAAACAACTCACCCTCATTGAAAGCGAAGCGGAAAA‑TCTTACCCGCCGCATTCGTAAAGCGGAACGGGACTACAAAACCCAGCGCGAATTGGTAACGGCGGCTAAAATGAGCTGGTGCGTGGTGCTGCGTTTATCTCGCAATTCTGAtg > 1:810943/1‑222 (MQ=255) aaCGTGAAAAAATCCGTCGGGATGAGTTGTATCAACAACTTTCCCCTAATCGTCAGTGCCGTTCTTACATTGAAAAACACCTCCCCCTCATTGAAAGCGAAGCGAAAAA‑TCTTACCCGCCGCATTCGTAAAGCGGAACGGGACTACAAAACCCAGCGCGAATTGGTAACGGCGGCTAAAATGAGCTGGTGCGTGGTGCTGCGTTTATCTCGCAATTCTGATGTGGAAAAACGCTTAAATCGTCGTGAAt < 1:82414/249‑1 (MQ=255) aCGTGAAAAAACCCGTCGGGATGATTTGTATCAACATCTTTCCACTAATCGTCAGTGCCTTTCTTACATTAAAAAACAACCCACCCTCATTGAAGGGGAAGGGAAAAA‑TCTTACCCGCCGCATTCGTAAAGCGGAACGGGACTACAAAACCCAGCGCGAATTGGTAACGGCGGCTAAAATGAGCTGGTg < 1:842106/189‑1 (MQ=255) cGTGCAAAAATCCGTCGGGATGAGTTGTATCAACAACTTTCCACTAATCGTCAGTGCCGTTCTTACATTGAAAAACACCTCACCCTCATTGAAAGCGAAGCGGAAAA‑TCTTACCCGCCGCATTCGTAAAGCGGAACGGGACTACAAAACCCAGCGCGAATTGGTAACGGCGGCTAAAATGAGCTGGTGCGTGGTGCTGCGTTTATCTCGCAATTCTGATGTGGAAAAACGCTTAAATCGTCGTGAATTGGc < 1:443489/251‑1 (MQ=255) gCAAAAACCCGTCGGGATGAGTTGTATCAACAACTTTCCACTAATCGTCAGTGCCGTTCTTACATTAAAAAACAACCCCCCCTCATTGAAAGCGAAGCGAAAAA‑TCTTACCCGCCGCATTCGTAAAGCGGAACGGGACTACAAAACCCAGCGCGAATTGGTAACGGCGGCTAAAATGAGCTGGTGCGTGGTGCTGCGTTTATCTCGCAATTCTGAtg < 1:1068285/217‑1 (MQ=255) aaaaTCCGTCGGGATGATTTGTATCAACAACTTCCCACTAATCTCCAGTGCCTTTTTTACATTAAAAAACAACCCCCCCTCATGAAAAGCAAGGCGAAAAA‑TCTTCCCCGCCGCATTCGTAAAGCGGAACGGGACTACAAAACCCAGCGCGAATTGGTAACGGCGGCTAAAATGAGCTGGTGCGTGGTGCTGCGTTTATCTCGCAATTCTGATGTGGAAAAACGCTTAAATCGTCGTGAATTGGCATAttt < 1:1077499/251‑1 (MQ=255) gggATGAGTTGTATCAACAACTTTCCACTAATCGTCAGTGCCGTTCTTACATTGAAAAACAACTCACCCTCATTGAAAGCGAAGCGGAAAA‑Tc < 1:770858/93‑1 (MQ=255) gAGTTGTAACAACAACTTTCCACTAATCGTCAGTGCCGTTCTTACATTAAAAAACAACTCCCCCTCATTAAAAGCGAAGCGGAAAA‑TCTTACCCGCCGCATTCGTAAAGCGGAACGGGACTACAAAACCCAGCGCGAATTGGTAACGGCGGCTAAAATGAGCTGGTGCGTGGTGCTGCGTTTATCTCGCAATTCTGATGTGGAAAAACGc < 1:461579/210‑1 (MQ=255) gTTGTATCAACAACTTTCCACTAATCGTCAGTGCCGTTCTTACATTGAAAAACAACTCACCCTCATTGAAAGCGAAGCGGAAAA‑TCTTACCCGCCGCATTCGTAAAGCGGAACGGGACTACAAAACCCAGCGCGAATTGGTAACGGCGGCTAAAATGAGCTGGTGCGTGGTGCTGCGTTTATCTCGCAATTCTGATGTGGAAAAACGCTTAAATCGTCGTGAATTGGCATATTTATccgc > 1:819593/1‑240 (MQ=255) aTCAACAACTTTCCACTAATCGTCAGTGCCGTTCTTACATTGAAAAACAACTCACCCTCATTGAAAGCGAAGCGGAAAA‑TCTTACCCGCCGCATTCGTAAAGCGGAACGGGACTACAAAAc > 1:649316/1‑121 (MQ=255) aaaacTTTCCACTAATCTACGGTGCCGTTCTTACATTGAAAAACAACCCCCCCTCATTAAAAGGAAAGCGAAAAA‑TCTTCCCCGCCGCATTCGTAAAGCGGAACGGGACTACAAAACCCAGCGCGAATTGGTAACGGCGGCTAAAATGAGCTGGTGCGTGGTGCTGCGTTTATCTCGCAATTCTGATGTGGAAAAACGCTTAAATCGTCGTGAATTGGCATATTTATCCGCCGATGAGCTACGTTCCATGt < 1:1097325/249‑1 (MQ=255) acTTTCCACTAATCGTCAGTGCCGTTCTTACATTGAAAAACAACTCACCCTCATTGAAAGCGAAGCGGAAAA‑TCTTACCCGCCGCATTCGTAAAGCGGAACGGGACTACAAAACCCAGCGCGAATTGGTAACGGCGGCTAAAATGAGCTGGTGCGTGGTGCTGCGTTTATCTCGCAATTCTGATGTGGAAAAACGCTTAAATCGTCGTGAATTGGCATATTTATCCGCCGATGAGCTACGTTCCATGTCgg > 1:1188516/1‑251 (MQ=255) acTTTCCACTAATCGTCAGTGCCGTTCTTACATTGAAAAACAACTCACCCTCATTGAAAGCGAAGCGGAAAA‑TCTTACCCGCCGCATTCGTAAAGCGGAACGGGACTACAAAACCCAGCGCGAATTGGTAACGGCGGCTAAAATGAGCTGGTGCGTGGTGCTGCGTTTATCTCGCAATTCTGATGTGGAAAAACGCTTAAATCGTCGTGAATTGGCATATTTATCCGCCGATGAGCTACGTTCCATGTCgg > 1:506749/1‑251 (MQ=255) cccACTAATCGTCAGTGCCGTTTTTACATAAAAAAACACCTCCCCCTCATGAAAAGCGAAGCGAAAAT‑TCTTACCCGCCGCATTCGTAAAGCGGAACGGGACTACAAAACCCAGCGCGAATTGGTAACGGCGGCTAAAATGAGCTGGTGCGTGGTGCTGCGTTTATCTCGCAATTCTGATGTGGAAAAACGCTTAAATCGTCGTGAATTGGCATATTTATCCGCCGATGAGCTACGTTCCATGTCGGACaa < 1:884311/250‑1 (MQ=255) ccACTAATCGTCAGTGCCGTTCTTACATTGAAAAACAACTCACCCTCATTGAAAGCGAAGCGGAAAA‑TCTTACCCGCCGCATTCGTAAAGCGGAACGGGACTACAAAACCCAGCGCGAATTGGTAACGGCGGCTAAAATGAGCTGGTGCGTGGTGCTGCGTTTATCTCGCAATTCTGATGTGGAAAAACGCTTAAATCGTCGTGAATTGGCATATTTATCCGCCGATGAGCTACGTTCCATGTCGGACaaa > 1:1130351/1‑251 (MQ=255) ccACTAATCGTCAGTGCCGTTCTTACATTGAAAAACAACTCACCCTCATTGAAAGCGAAGCGGAAAA‑TCTTACCCGCCGCATTCGTAAAGCGGAACGGGACTACAAAACCCAGCGCGAATTGGTAACGGCGGCTAAAATGAGCTGGTGCGTGGTGCTGCGTTTATCTCGCAATTCTGATGTGGAAAAACGCTTAAATCGTCGTGAATTGGCATATTTATCCGCCGATGAGCTACGTTCCATGTCGGACaaa > 1:661179/1‑251 (MQ=255) ctaaaccGTCAGTGCCGTTCTTACATAAAAAAACAACCCCCCCTCAGTGAAAG‑GGAGGGGAAAAGTCTTCCCCGCCGCATTCGTAAAGCGGAACGGGACTACAAAACCCAGCGCGAATTGGTAACGGCGGCTAAAATGAGCTGGTGCGTGGTGCTGCGTTTATCTCGCAATTCTGATGTGGAAAAACGCTTAAATCGTCGTGAATTGGCATATTTATCCGCCGATGAGCTACGTTCCATGTCGGACAAAGc < 1:649102/245‑1 (MQ=255) aCTAATCGTCAGTGCCGTTCTTACATTGAAAAACAACTCACCCTCATTGAAAGCGAAGCGGAAAA‑TCTTACCCGCCGCATTCGTAAAGCGGAACGGGACTACAAAACCCAGCGCGAATTGGTAACGGCGGCTAAAATGAGCTGGTGCGTGGTGCTGCGTTTATCTCGCAATTCTGATGTGGAAAAACGCTTAAATCGTCGTGAATTGGCATATTTATCCGCCGATGAGCTACGTTCCATGTCGGACAAAg > 1:950446/1‑250 (MQ=255) cTAATCGTCAGTGCCGTTCTTACATTGAAAAACAACTCACCCTCATTGAAAGCGAAGCGGAAAA‑TCTTAccc < 1:429824/72‑1 (MQ=255) cTAATCGTCAGTGCCGTTCTTACATTGAAAAACAACTCACCCTCATTGAAAGCGAAGCGGAAAA‑TCTTACCCGCCGCATTCGTAAAGCGGAACGGGACTACAAAACCCAGCGCGAATTGGTAACGGCGGCTAAAATGAGCTGGTGCGTGGTGCTGCGTTTATCTCGCAATTCTGATGTGGAAAAACGCTTAAATCGTCGTGAATTGGCATATTTATCCGCCGATGAGCTACGTTCCATGt > 1:14305/1‑240 (MQ=255) cGTCAGTGCCGTTCTTACATTGAAAAACAACTCACCCTCATTGAAAGCGAAGCGGAAAA‑TCTTACCCGCCGCATTCGTAAAGCGGAACGGGACTACAAAACCCAGCGCGAATTGGTAACGGCGGCTAAAATGAGCTGGTGCGTGGGGCTGCGTTTATCTCGCAATTCTGATGTGGAAAAACGCTTAAATCGTCGTGAATTGGCATAttt > 1:144877/1‑209 (MQ=255) tCAGTGCCGTTCTTACATTGAAAAACAACTCACCCTCATTGAAAGCGAAGCGGAAAA‑TCTTACCCGCCGCATTCGTAAAGCGGAACGGGACTACAAAACCCAGCGCGAATTGGTAACGGCGGCTAAAATGAGCTGGTGCGTGGTGCTGCGTTTATCTCGCAATTCTGATGTGGAAAAACGCTTAAATCGTCGTGAATTGGCATATTTATCCGCCGATGAGCTACGTTCCATGTCGGACAAAGCCCTTGGtg > 1:1065645/1‑251 (MQ=255) ccAGTGCCGTTCTTACATTAAAAAACAACCCCCCCACATGAAAAGCAAAGCGAAAAA‑TCTTCCCCGCCGCATTCGTAAAGCGGAACGGGACTACAAAACCCAGCGCGAATTGGTAACGGCGGCTAAAATGAGCTGGTGCGTGGTGCTGCGTTTATCTCGCAATTCTGATGTGGAAAAACGCTTAAATCGTCGTGAATTGGCATATTTATCCGCCGATGAGCTACGTTCCATGTCGGACAAAGCCCTTGGtg < 1:908888/250‑1 (MQ=255) gTGCCGTTCTTACATTGAAAAACAACTCACCCTCATTGAAAGCGAAGCGGAAAA‑TCTTACCCGCCGCATTCGTAAAGCGGAACGGGACTACAAAACCCAGCGCGAATTGGTAACGGCGGCTAAAATGAGCTGGTGCGTGGTGCTGCGTTTATCTCGCAATTCTGATGTGGAAAAACGCTTAAATCGTCGTGAATTGGCATATTTATCCGCCGATGAGCTACGTTCCATGTCGGACAAAGCCCTTGGtgcgt > 1:639386/1‑251 (MQ=255) gTGCCGTTCTTACATTAAAAAACACCCCCCCCTCATTAAAAGCAAAGCGAAAAA‑TCTTCCCCGCCGCATTCGTAAAGCGGAACGGGACTACAAAACCCAGCGCGAATTGGTAACGGCGGCTAAAATGAGCTGGTg < 1:1154364/135‑1 (MQ=255) ccctttCTTACATTAAAAACCACCCCCCCCTCATAGAAAGCAAAGCGAAAAT‑TCTTCCCCGCCGCATTCGTAAAGCGGAACGGGACTACAAAACCCAGCGCGAATTGGTAACGGCGGCTAAAATGAGCTGGTGCGTGGTGCTGCGTTTATCTCGCAATTCTGATGTGGAAAAACGCTTAAATCGTCGTGAATTGGCATATTTATCCGCCGATGAGCTACGTTCCATGTCGGACAAAGCCCTTGGtgcgttg < 1:501621/247‑1 (MQ=255) aTTGAAAAACAACTCACCCTCATTGAAAGCGAAGCGGAAAA‑TCTTACCCGCCGCATTCGTAAAGCGGAACGGGACTACAAAACCCAGCGCGAATTGGTAACGGCGGCTAAAATGAGCTGGTGCGTGGTGCTGCGTTTATCTCGCAATTCTGATGTGGAAAAACGCTTAAATCGTCGTGAATTGGCATATTTATCCGCCGATGAGCTACGTTCCATGTCGGACAAAGCCCTTGGTGCGTTGCGTAcggcggg > 1:685364/1‑250 (MQ=255) | CGAAACACAGCGCGAGCAATTGCGTCAGGTTCAAGCGCAATATGCACAATACAATCAAGTGTTGATTCAATTGCAAAGTTCTTTCAACAGT‑AAAAACCAAATGTTGCAGGAATTAATGCAGGAAATCGGCGAATTGGGT‑GTACGTGCCGACGAAGGCGCGGAAGAACGTGCAAAAATCCGTCGGGATGAGTTGTATCAACAACTTTCCACTAATCGTCAGTGCCGTTCTTACATTGAAAAACAACTCACCCTCATTGAAAGCGAAGCGGAAAA‑TCTTACCCGCCGCATTCGTAAAGCGGAACGGGACTACAAAACCCAGCGCGAATTGGTAACGGCGGCTAAAATGAGCTGGTGCGTGGTGCTGCGTTTATCTCGCAATTCTGATGTGGAAAAACGCTTAAATCGTCGTGAATTGGCATATTTATCCGCCGATGAGCTACGTTCCATGTCGGACAAAGCCCTTGGTGCGTTGCGTACGGCGGT > 1/1454961‑1455443 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |