New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	1	250645 =	73 (0.590)	4 (0.030)	4/430	9.2	5.2%	intergenic (+85809/+40098)	unknown/unknown	tRNA‑Val‑TAC/LSU rRNA ## 23S rRNA, large subunit ribosomal RNA
	?	1	250669 =	74 (0.610)					intergenic (+85833/+40074)	unknown/unknown	tRNA‑Val‑TAC/LSU rRNA ## 23S rRNA, large subunit ribosomal RNA
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

AGAGTTTCCAGCGTGGTATAACCGGCTTGTGCCAATTTCGCCAATAATTCAAGAGAAAGTTCCATATTCGCCGGGCAAATTAATTCGCCGGTTTCTAAATCTACATAATCTTTCGCGGCAACTTTCCCGATAATATATTCGGTTGGCACTTCCACCTGAGTGACTTTATCTTTTTCCAACGCTTTAATGTGACGGGCGGTAATACGACGACCGCGTTCTACATACACCTTGCCTTTGGTTTCAATATCAAAGGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  1/250898‑250645 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑caaaggcAAGGTGTATGTAGAACGCGGTCGTCGTATTACCGCCCGTCACATTAAAGC  >  1/250669‑250718                                                                                                                                                                                                                                                                                                                  AGAGTTTCCAGCGTGGTATAACCGGCTTGTGCCAATTTCGCCAATAATTCAAGAGAAAGTTCCATATTCGCCGGGCAAATTAATTCGCCGGTTTCTAAATCTACATAATCTTTCGCGGCAACTTTCCCGATAATATATTCGGTTGGCACTTCCACCTGAGTGACTTTATCTTTTTCCAACGCTTTAATGTGACGGGCGGTAATACGACGACCGCGTTCTACATACACCTTGCCTTTGGTTTCAATATCAAA                                                       >  1:253277/1‑251     TTTCCAGCGTGGTATAACCGGCTTGTGCCAATTTCGCCAATAATTCAAGAGAAAGTTCCATATTCGCCGGGCAAATTAATTCGCCGGTTTCTAAATCTACATAATCTTTCGCGGCAACTTTCCCGATAATATATTCGGTTGGCACTTCCACCTGAGTGACTTTATCTTTTTCCAACGCTTTAATGTGACGGGCGGTAATACGACGACCGCGTTCTACATACACCTTGCCTTTGGTTTCAATATCAAAGGC                                                    <  1:555681/250‑1     TTTCCAGCGTGGTATAACCGGCTTGTGCCAATTTCGCCAATAATTCAAGAGAAAGTTCCATATTCGCCGGGCAAATTAATTCGCCGGTTTCTAAATCTACATAATCTTTCGCGGCAACTTTCCCGATAATATATTCGGTTGGCACTTCCACCTGAGTGACTTTATCTTTTTCCAACGCTTTAATGTGACGGGCGGTAATACGACGACCGCGTTCTACATACACCTTGCCTTTGGTTTCAATATCAAAGGC                                                    <  1:872084/250‑1              TGGTATAACCGGCTTGTGCCAATTTCGCCAATAATTCAAGAGAAAGTTCCATATTCGCCGGGCAAATTAATTCGCCGGTTTCTAAATCTACATAATCTTTCGCGGCAACTTTCCCGATAATATATTCGGTTGGCACTTCCACCTGAGTGACTTTATCTTTTTCCAACGCTTTAATGTGACGGGCGGTAATACGACGACCGCGTTCTACATACACCTTGCCTTTGGTTTCAATATCAAAG                                                      >  1:701694/1‑239                                CCAATTTCGCCAATAATTCAAGAGAAAGTTCCATATTCGCCGGGCAAATTAATTCGCCGGTTTCTAAATCTACATAATCTTTCGCGGCAACTTTCCCGATAATATATTCGGTTGGCACTTCCACCTGAGTGACTTTATCTTTTTCCAACGCTTTAATGTGACGGGCGGTAATACGACGACCGCGTTCTACATACACCTTGCCTTTGGTTTCAATATCAAAGGCAAGGTGTATGTAGAACGCGGTCGTCGTA                        <  1:1025676/251‑1                                                                                                                      GCAACTTTCCCGATAATATATTCGGTTGGCACTTCCACCTGAGTGACTTTATCTTTTTCCAACGCTTTAATGTGACGGGCGGTAATACGACGACCGCGTTCTACATACACCTTGCCTTTGGTTTCAATATCAAAGGCAAGGTGTATGTAGAACGCGGTCGTCGTATTAC                    >  1:728003/1‑169                                                                                                                                CGATAATATATTCGGTTGGCACTTCCACCTGAGTGACTTTATCTTTTTCCAACGCTTTAATGTGACGGGCGGGAATACGACGACCGCGTTCTACATACACCTTGCCTTTGGTTTCAATATCAAAGGGAAGGTGTATGTAGAACGCGGTCGTCGTATTACCGCCCGTCACATTAAAac  >  1:793580/1‑175                                                                                                                                                                  GACTTTATCTTTTTCCAACGCTTTAATGTGACGGGCGGTAATACGACGACCGCGTTCTACATACACCTTGCCTTTGGTTTCAATATCAAAGGCAAGGTGTATGTAGAACGCGGTCGTCGTATTAC                    <  1:1075602/125‑1                                                                                                                                                                                                                                                                                                                  AGAGTTTCCAGCGTGGTATAACCGGCTTGTGCCAATTTCGCCAATAATTCAAGAGAAAGTTCCATATTCGCCGGGCAAATTAATTCGCCGGTTTCTAAATCTACATAATCTTTCGCGGCAACTTTCCCGATAATATATTCGGTTGGCACTTCCACCTGAGTGACTTTATCTTTTTCCAACGCTTTAATGTGACGGGCGGTAATACGACGACCGCGTTCTACATACACCTTGCCTTTGGTTTCAATATCAAAGGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  1/250898‑250645 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑caaaggcAAGGTGTATGTAGAACGCGGTCGTCGTATTACCGCCCGTCACATTAAAGC  >  1/250669‑250718

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.