Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	863,092	G→A	intergenic (‑11894/‑60625)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	863,092	0	G	A	100.0%	161.3 / NA	39	intergenic (‑11894/‑60625)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (16/23);  total (16/23)

ATACTGCAACGATTAACACGGCAACAGCAATAGCGATCAAACCATATTCAATTGCAGTCACACCTGCTTGATTTTCTCTGAAAGAACGGATTGCTTCAGCTGCTTTGATGTATGCTTTAGTAGTTAAAGTATTTAACATTGTCTTAATGCTCCTTTGTTGTTTATAGAAAATAAAATTCAAGAATCCTTGAGTTTGAACTTAGTGTCATTTATTTAATAAGCTTGTGGAATTAAATAAACGACCGGTGTTATAGTCCTGAAAAATTAAAATGCAAGTTTTTTTAGAATAATAAATTCACTATGTATATGTACGTATTGTGATTGCGAAAACTTTTTAATTAACAGTATGTTAAGGGTTATTGTAAATGAGCTATTTCTACTTTCTCTATTGATTTTACAGTAAATTTACAACTTTTAACTTTAATTACAAAAACACTTAAATATGTGTGGATTTAGAGAT  >  1/862845‑863304                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                     aTACTGCAACGATTAACACGGCAACAGCAATAGCGATCAAACCATATTCAATTGCAGTCACACCTGCTTGATTTTCTCTGAAAGAACGGATTGCTTCAGCTGCTTTGATGTATGCTTTAGTAGTTAAAGTATTTAACATTGTCTTAATGCTCCTTTGTTGTTTATAGAAAATAAAATTCAAGAATCCTTGAGTTTGAACTTAGTGTCATTTATTTAATAAGCTTGTGGAATTAAATAAACGACCGGTATta                                                                                                                                                                                                                   >  1:547877/1‑251 (MQ=255)   ccTGCAACGATTAACACGGCAACAGCAATAGCGATCAAACCATATTCAATTGCAGTCACACCTGCTTGATTTTCTCTGAAAGAACGGATTGCTTCAGCTGCTTTGATGTATGCTTTATTAGTTAAAGTATTTAACATTGTCTTAATGCTCCTTTGTTGTTTATAGAAAATAAAATTCAAGAATCCTTGAG‑TTGAACTTAGTGTCATTTATTTAATAAGCTTGTGGAATTAAATAAACGACCGGTATTATAg                                                                                                                                                                                                                <  1:385521/250‑1 (MQ=255)    cTGCAACGATTAACACGGCAACAGCAATAGCGATCAAACCATATTCAATTGCAGTCACACCTGCTTGATTTTCTCTGAAAGAACGGATTGCTTCAGCTGCTTTGATGTATGCTTTAGTAGTTAAAGTATTTAACATTGTCTTAATGCTCCTTTGTTGTTTATAGAAAATAAAATTCAAGAATCCTTGAGTTTGAACTTAGTGTCATTTATTTAATAAGCTTGTGGAATTAAATAAACGACCGGTATTATAg                                                                                                                                                                                                                <  1:650261/251‑1 (MQ=255)       cAACGATTAACACGGCAACAGCAATAGCGATCAAACCATATTCAATTGCAGTCACACCTGCTTGATTTTCTCTGAAAGAACGGATTGCTTCAGCTGCTTTGATGTATGCTTTAGTAGTTAAAGTATTTAACATTGTCTTAATGCTCCTTTGTTGTTTATAGAAAATAAAATTCAAGAATCCTTGAGTTTGAACTTAGTGTCATTTATTTAATAAGCTTGTGGAATTAAATAAACGACCGGTATTATAGTcc                                                                                                                                                                                                             <  1:521936/251‑1 (MQ=255)           gATTAACACGGCAACAGCAATAGCGATCAAACCATATTCAATTGCAGTCACACCTGCTTGATTTTCTCTGAAAGAACGGATTGCTTCAGCTGCTTTGATGTATGCTTTAGTAGTTAAAGTATTTAACATTGTCTTAATGCTCCTTTGTTGTTTATAGAAAATAAAATTCAAGAATCCTTGAGTTTGAACTTAGTGTCATTTATTTAATAAGCTTGTGGAATTAAATAAACGACCGGTATTATAg                                                                                                                                                                                                                <  1:394575/244‑1 (MQ=255)                           gCAATAGCGATCAAACCATATTCAATTGCAGTCACACCTGCTTGATTTTCTCTGAAAGAACGGATTGCTTCAGCTGCTTTGATGTATGCTTTAGTAGTTAAAGTATTTAACATTGTCTTAATGCTCCTTTGTTGTTTATAGAAAATAAAATTCAAGAATCCTTGAGTTTGAACTTAGTGTCATTTATTTAATAAGCTTGTGGAATTAAATAAACGACCGGTATTATAg                                                                                                                                                                                                                >  1:615950/1‑228 (MQ=255)                                        aaCCATATTCAATTGCAGTCACACCTGCTTGATTTTCTCTGAAAGAACGGATTGCTTCAGCTGCTTTGATGTATGCTTTAGTAGTTAAAGTATTTAACATTGTCTTAATGCTCCTTTGTTGTTTATAGAAAATAAAATTCAAGAATCCTTGAGTTTGAACTTAGTGTCATTTATTTAATAAGCTTGTGGAATTAAATAAACGACCGGTATTATAg                                                                                                                                                                                                                <  1:103266/215‑1 (MQ=255)                                         aCCATATTCAATTGCAGTCACACCTGCTTGATTTTCTCTGAAAGAACGGATTGCTTCAGCTGCTTTGATGTATGCTTTAGTAGTTAAAGTATTTAACATTGTCTTAATGCTCCTTTGTTGTTTATAGAAAATAAAATTCAAGAATCCTTGAGTTTGAACTTAGTGTCATTTATTTAATAAGCTTGTGGAATTAAATAAACGACCGGTATTATAGTCCTGAAAAATTAAAATGCAAGttttttt                                                                                                                                                                                   >  1:820445/1‑243 (MQ=255)                                               ttCAATTGCAGTCACACCTGCTTGATTTTCTCTGAAAGAACGGATTGCTTCAGCTGCTTTGATGTATGCTTTAGTAGTTAAAGTATTTAACATTGTCTTAATGCTCCTTTGTTGTTTATAGAAAATAAAATTCAAGAATCCTTGAGTTTGAACTTAGTGTCATTTATTTAATAAGCTTGTGGAATTAAATAAACGACCGGTATTATAGTCCTGAAAAATTAAAATGCAAGTTTTTTTAGAATAATAAATTc                                                                                                                                                                     <  1:141917/251‑1 (MQ=255)                                                 cAATTGCAGTCACACCTGCTTGATTTTCTCTGAAAGAACGGATTGCTTCAGCTGCTTTGATGTATGCTTTAGTAGTTAAAGTATTTAACATTGTCTTAATGCTCCTTTGTTGTTTATAGAAAATAAAATTCAAGAATCCTTGAGTTTGAACTTAGTGTCATTTATTTAATAAGCTTGTGGAATTAAATAAACGACCGGTATTATAGTCCTGAAAAATTAAAATGCAAGTTTTTTTAGAATAATAAATTCa                                                                                                                                                                    >  1:289883/1‑250 (MQ=255)                                                     tGCAGTCACACCTGCTTGATTTTCTCTGAAAGAACGGATTGCTTCAGCTGCTTTGATGTATGCTTTAGTAGTTAAAGTATTTAACATTGTCTTAATGCTCCTTTGTTGTTTATAGAAAATAAAATTCAAGAATCCTTGAGTTTGAACTTAGTGTCATTTATTTAATAAGCTTGTGGAATTAAATAAACGACCGGTATTATAGTCCTGAAAAATTAAAATGCAAGTTTTTTTAGAATAATAAATTCACtatg                                                                                                                                                               <  1:670304/251‑1 (MQ=255)                                                       cAGTCACACCTGCTTGATTTTCTCTGAAAGAACGGATTGCTTCAGCTGCTTTGATGTATGCTTTAGTAGTTAAAGTATTTAACATTGTCTTAATGCTCCTTTGTTGTTTATAGAAAATAAAATTCAAGAATCCTTGAGTTTGAACTTAGTGTCATTTATTTAATAAGCTTGTGGAATTAAATAAACGACCGGTATTATAGTCCTGAAAAATTAAAATGCAAGTTTTTTTAGAATAATAAATTCACtatgta                                                                                                                                                             >  1:617607/1‑251 (MQ=255)                                                           cacaCCTGCTTGATTTTCTCTGAAAGAACGGATTGCTTCAGCTGCTTTGATGTATGCTTTAGTAGTTAAAGTATTTAACATTGTCTTAATGCTCCTTTGTTGTTTATAGAAAATAAAATTCAAGAATCCTTGAGTTTGAACTTAGTGTCATTTATTTAATAAGCTTGTGGAATTAAATAAACGACCGGTATTATAGTCCTGAAAAATTAAAATGCAAGTTTTTTTAGAATAATAAATTCACtatgtatatg                                                                                                                                                         <  1:75308/251‑1 (MQ=255)                                                           cacaCCTGCTTGATTTTCTCTGAAAGAACGGATTGCTTCAGCTGCTTTGATGTATGCTTTAGTAGTTAAAGTATTTAACATTGTCTTAATGCTCCTTTGTTGTTTATAGAAAATAAAATTCAAGAATCCTTGAGTTTGAACTTAGTGTCATTTATTTAATAAGCTTGTGGAATTAAATAAACGACCGGTATTATAGTCCTGAAAAATTAAAATGCAAGTTTTTTTAGAATAATAAATTCACtatgtatatg                                                                                                                                                         <  1:445196/251‑1 (MQ=255)                                                             cacCTGCTTGATTTTCTCTGAAAGAACGGATTGCTTCAGCTGCTTTGATGTATGCTTTAGTAGTTAAAGTATTTAACATTGTCTTAATGCTCCTTTGTTGTTTATAGAAAATAAAATTCAAGAATCCTTGAGTTTGAACTTAGTGTCATTTATTTAATAAGCTTGTGGAATTAAATAAACGACCGGTATTATAGTCCTGAAAAATTAAAATGCAAGTTTTTTTAGAATAATAAATTCACtatgtatatgta                                                                                                                                                       <  1:110932/251‑1 (MQ=255)                                                                           tctcTGAAAGAACGGATTGCTTCAGCTGCTTTGATGTATGCTTTAGTAGTTAAAGTATTTAACATTGTCTTAATGCTCCTTTGTTGTTTATAGAAAATAAAATTCAAGAATCCTTGAGTTTGAACTTAGTGTCATTTATTTAATAAGCTTGTGGAATTAAATAAACGACCGGTATTATAGTCCTGAAAAATTAAAATGCAAGTTTTTTTAGAATAATAAATTCACTATGTATATGTACGTATTGTGATTg                                                                                                                                          >  1:50341/1‑250 (MQ=255)                                                                                                    ctgctTTGATGTATGCTTTAGTAGTTAAAGTATTTAACATTGTCTTAATGCTCCTTTGTTGTTTATAGAAAATAAAATTCAAGAATCCTTGAGTTTGAACTTAGTGTCATTTATTTAATAAGCTTGTGGAATTAAATAAACGACCGGTATTATAGTCCTGAAAAATTAAAATGCAAGTTTTTTTAGAATAATAAATTCACTATGTATATGTACGTATTGTGATTGCGAAAACTTTTTAATTAACAG‑ATGtt                                                                                                               <  1:649669/251‑1 (MQ=255)                                                                                                      gctTTGATGTATGCTTTAGTAGTTAAAGTATTTAACATTGTCTTAATGCTCCTTTGTTGTTTATAGAAAATAAAATTCAAGAATCCTTGAGTTTGAACTTAGTGTCATTTATTTAATAAGCTTGTGGAATTAAATAAACGACCGGtattat                                                                                                                                                                                                                  >  1:184799/1‑151 (MQ=255)                                                                                                                   cTTTAGTAGTTAAAGTATTTAACATTGTCTTAATGCTCCTTTGTTGTTTATAGAAAATAAAATTCAAGAATCCTTGAGTTTGAACTTAGTGTCATTTATTTAATAAGCTTGTGGAATTAAATAAACGACCGGTATTATAGTCCTGAAAAATTAAAATGCAAGTTTTTTTAGAATAATAAATTCACtatgtatatgta                                                                                                                                                       <  1:363263/197‑1 (MQ=255)                                                                                                                         tagtTAAAGTATTTAACATTGTCTTAATGCTCCTTTGTTGTTTATAGAAAATAAAATTCAAGAATCCTTGAGTTTGAACTTAGTGTCATTTATTTAATAAGCTTGTGGAATTAAATAAACGACCGGTATTATAGTCCTGAAAAATTAAAATGCAAGTTTTTTTAGAATAATAAATTCACtatgtatatg                                                                                                                                                         >  1:122238/1‑189 (MQ=255)                                                                                                                                 gTATTTAACATTGTCTTAATGCTCCTTTGTTGTTTATAGAAAATAAAATTCAAGAATCCTTGAGTTTGAACTTAGTGTCATTTATTTAATAAGCTTGTGGAATTAAATAAACGACCGGTATTATAGTcc                                                                                                                                                                                                             >  1:493250/1‑129 (MQ=255)                                                                                                                                 gTATTTAACATTGTCTTAATGCTCCTTTGTTGTTTATAGAAAATAAAATTCAAGAATCCTTGAGTTTGAACTTAGTGTCATTTATTTAATAAGCTTGTGGAATTAAATAAACGACCGGTATTATAGTCCTGAAAAATTAAAATGCAAGTTTTTTTAGAATAATAAATTCACTATGTATATGTACGTATTGTGATTGCGAAAACTTTTTAATTAACAGTATGTTAAGGGTTATTGTAAATGAGCTATTTCTa                                                                                   >  1:640494/1‑251 (MQ=255)                                                                                                                                   aTTTAACATTGTCTTAATGCTCCTTTGTTGTTTATAGAAAATAAAATTCAAGAATCCTTGAGTTTGAACTTAGTGTCATTTATTTAATAAGCTTGTGGAATTAAATAAACGACCGGTATTATAGTCCTGAAAAATTAAAATGCAAGTTTTTTTAGAATAATAAATTCACTATGTATATGTACGTATTGTGATTGCGAAAACTTTTTAATTAACAGTATGTTAAGGGTTATTGTAAATGAGCTATTTCTa                                                                                   >  1:99286/1‑249 (MQ=255)                                                                                                                                   aTTTAACATTGTCTTAATGCTCCTTTGTTGTTTATAGAAAATAAAATTCAAGAATCCTTGAGTTTGAACTTAGTGTCATTTATTTAATAAGCTTGTGGAATTAAATAAACGACCGGTATTATAGTCCTGAAAAATTAAAATGCAAGTTTTTTTAGAATAATAAATTCACTATGTATATGTACGTATTGTGATTGCGAAAACTTTTTAATTAACAGTATGTTAAGGGTTATTGTAAATGAGCTATTTCTACt                                                                                 <  1:406703/251‑1 (MQ=255)                                                                                                                                        aCATTGTCTTAATGCTCCTTTGTTGTTTATAGAAAATAAAATTCAAGAATCCTTGAGTTTGAACTTAGTGTCATTTATTTAATAAGCTTGTGGAATTAAATAAACGACCGGTATTATAGTcc                                                                                                                                                                                                             >  1:797339/1‑122 (MQ=255)                                                                                                                                        aCATTGTCTTAATGCTCCTTTGTTGTTTATAGAAAATAAAATTCAAGAATCCTTGAGTTTGAACTTAGTGTCATTTATTTAATAAGCTTGTGGAATTAAATAAACGACCGGTATTATAGTCCTGAAAAATTAAAATGCAAGTTTTTTTAGAATAATAAATTCACtatgtatatg                                                                                                                                                         <  1:77003/174‑1 (MQ=255)                                                                                                                                             gTCTTAATGCTCCTTTGTTGTTTATAGAAAATAAAATTCAAGAATCCTTGAGTTTGAACTTAGTGTCATTTATTTAATAAGCTTGTGGAATTAAATAAACGACCGGTATTATAg                                                                                                                                                                                                                >  1:38715/1‑114 (MQ=255)                                                                                                                                             gTCTTAATGCTCCTTTGTTGTTTATAGAAAATAAAATTCAAGAATCCTTGAGTTTGAACTTAGTGTCATTTATTTAATAAGCTTGTGGAATTAAATAAACGACCGGTATTATAGTCCTGAAAAATTAAAATGCAAGTTTTTTTAGAATAATAAATTCACtatgtatatg                                                                                                                                                         <  1:622933/169‑1 (MQ=255)                                                                                                                                             gTCTTAATGCTCCTTTGTTGTTTATAGAAAATAAAATTCAAGAATCCTTGAGTTTGAACTTAGTGTCATTTATTTAATAAGCTTGTGGAATTAAATAAACGACCGGTATTATAGTCCTGAAAAATTAAAATGCAAGTTTTTTTAGAATAATAAATTCACTATGTATATGTACGTATTGTGAt                                                                                                                                            >  1:99646/1‑182 (MQ=255)                                                                                                                                                        ccTTTGTTGTTTATAGAAAATAAAATTCAAGAATCCTTGAGTTTGAACTTAGTGTCATTTATTTAATAAGCTTGTGGAATTAAATAAACGACCGGTATTATAGTCCTGAAAAATTAAAATGCAAGTTTTTTTAGAATAATAAATTcc                                                                                                                                                                    >  1:395903/1‑146 (MQ=255)                                                                                                                                                        ccTTTGTTGTTTATAGAAAATAAAATTCAAGAATCCTTGAGTTTGAACTTAGTGTCATTTATTTAATAAGCTTGTGGAATTAAATAAACGACCGGTATTATAGTCCTGAAAAATTAAAATGCAAGTTTTTTTAGAATAATAAATTCACtatgtata                                                                                                                                                           <  1:645654/156‑1 (MQ=255)                                                                                                                                                             gttgttTATAGAAAATAAAATTCAAGAATCCTTGAGTTTGAACTTAGTGTCATTTATTTAATAAGCTTGTGGAATTAAATAAACGACCGGTATTATAg                                                                                                                                                                                                                >  1:804552/1‑98 (MQ=255)                                                                                                                                                                           ataaaatTCAAGAATCCTTGAGTTTGAACTTAGTGTCATTTATTTAATAAGCTTGTGGAATTAAATAAACGACCGGTATTATAGTCCTGAAAAATTAAAATGCAAGTTTTTTTAGAATAATAAATTCACTATGTATATGTACGTATTGTGAt                                                                                                                                            <  1:421272/152‑1 (MQ=255)                                                                                                                                                                                                gTTTGAACTTAGTGTCATTTATTTAATAAGCTTGTGGAATTAAATAAACGACCGGTATTATAGTCCTGAAAAATTAAAATGCAAGTTTTTTTAGAATAATAAATTCACtatgtata                                                                                                                                                           <  1:769154/116‑1 (MQ=255)                                                                                                                                                                                                      aCTTAGTGTCATTTATTTAATAAGCTTGTGGAATTAAATAAACGACCGGTATTATAGTCCTGAAAAATTAAAATGCAAGTTTTTTTAGAATAATAAATTCACTATGTATATGTACGTATtgtg                                                                                                                                              >  1:378279/1‑123 (MQ=255)                                                                                                                                                                                                                atttatttAATAAGCTTGTGGAATTAAATAAACGACCGGTATTATAGTCCTGAAAAATTAAAATGCAAGTTTTTTTAGAATAATAAATTCACTATGTATATGTACGTATTGTGATTGCGAAAACTTTTTAATTAACAGTATGTTAAGGGTTATTGTAAATGAGCTATTTCTACTTTCTCTATTGATTTTACAGTAAATTTACAACTTTTAACTTTAATTACAAAAACACTTAAATATGTGTGGATTTagag    <  1:203598/251‑1 (MQ=255)                                                                                                                                                                                                                atttatttAATAAGCTTGTGGAATTAAATAAACGACCGGTATTATAGTCCTGAAAAATTAAAATGCAAGTTTTTTTAGAATAATAAATTCACTATGTATATGTACGTATTGTGATTGCGAAAACTTTTTAATTAACAGTATGTTAAGGGTTATTGTAAATGAGCTATTTCTACTTTCTCTATTGATTTTACAGTAAATTTACAACTTTTAACTTTAATTACAAAAACACTTAAATATGTGTGGATTTagag    <  1:246067/251‑1 (MQ=255)                                                                                                                                                                                                                atttatttAATAAGCTTGTGGAATTAAATAAACGACCGGTATTATAGTCCTGAAAAATTAAAATGCAAGTTTTTTTAGAATAATAAATTCACTATGTATATGTACGTATTGTGATTGCGAAAACTTTTTAATTAACAGTATGTTAAGGGTTATTGTAAATGAGCTATTTCTACTTTCTCTATTGATTTTACAGTAAATTTACAACTTTTAACTTTAATTACAAAAACACTTAAATATGTGTGGATTTagag    <  1:635079/251‑1 (MQ=255)                                                                                                                                                                                                                  ttatttaATAAGCTTGTGGAATTAAATAAACGACCGGTATTATAGTCCTGAAAAATTAAAATGCAAGTTTTTTTAGAATAATAAATTCACTATGTATATGTACGTATTGTGATTGCGAAAACTTTTTAATTAACAGTATGTTAAGGGTTATTGTAAATGAGCTATTTCTACTTTCTCTATTGATTTTACAGTAAATTTACAACTTTTAACTTTAATTACAAAAACACTTAAATATGTGTGGATTTAGAGat  <  1:651302/251‑1 (MQ=255)                                                                                                                                                                                                                                                    cGGTATTATAGTCCTGAAAAATTAAAATGCAAGTTTTTTTAGAATAATAAATTCACTATGTATATGTACGTATTGTGATTGCGAAAACTTTTTAATTAACAGTATGTTAAGGGTTATTGTAAATGAGCTATTTCTACTTTCTCTATTGATTTTACAGTAAATTTACAACTTTTAACTTTAATTACAAAAACACTTAAATATGTGTGGAt          <  1:427766/209‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                     ATACTGCAACGATTAACACGGCAACAGCAATAGCGATCAAACCATATTCAATTGCAGTCACACCTGCTTGATTTTCTCTGAAAGAACGGATTGCTTCAGCTGCTTTGATGTATGCTTTAGTAGTTAAAGTATTTAACATTGTCTTAATGCTCCTTTGTTGTTTATAGAAAATAAAATTCAAGAATCCTTGAGTTTGAACTTAGTGTCATTTATTTAATAAGCTTGTGGAATTAAATAAACGACCGGTGTTATAGTCCTGAAAAATTAAAATGCAAGTTTTTTTAGAATAATAAATTCACTATGTATATGTACGTATTGTGATTGCGAAAACTTTTTAATTAACAGTATGTTAAGGGTTATTGTAAATGAGCTATTTCTACTTTCTCTATTGATTTTACAGTAAATTTACAACTTTTAACTTTAATTACAAAAACACTTAAATATGTGTGGATTTAGAGAT  >  1/862845‑863304

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑