Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	2,138,106	0	C	A	43.1%	80.3 / 23.7	58	intergenic (‑18161/–)	unknown/–	tRNA‑Met‑CAT/–
Reads supporting (aligned to +/- strand):  ref base C (24/9);  new base A (0/25);  total (24/34)
Fisher's exact test for biased strand distribution p-value = 3.10e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GCGTATAAGCGGCTAAAAGTGCGGCCAAAATCCAAACGATATTTTGCTCGCCGAAAAAGCCGATCACATTGCCGAACAAAGTCACGCCCACCACAAACGCCAGAGAACCGATTAACCGCGCTTTGCCG‑TAATCCAGTT‑G‑AATCTGGCGCTGCCAGGTGCTCGCCAGCGAATCCGTAATCGGCATACCGGCGGCGTTTA‑CCATGGCAAACAAA‑CCGATAGCGGAGAATAACAGCCAAAAA‑TT‑CTCGGCAACAAAA‑CTGAGCCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCTGCGACGCCCGTTTAATTAAACCGGAGAAGAACAAACCGCCCACAAAACGGAACACATAAGCACTCGCCAGCACCATGCCGATCATTTCCTCGCCGTATTGTTGGGATTTCATCCAAACGGGAAAAAACGGCACGAATACGCCATAAGCGCAGTAGTAGCCGAAAAAGCTCAAGGCGAGCC  >  1/2137857‑2138351                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                       gCGTATAAGCGGCTAAAAGTGCGGCCAAAATCCAAACGATATTTTGCTCGCCGAAAAAGCCGATCACATTGCCGAACAAAGTCACGCCCACCACAAACGCCAGAGAACCGATTAACCGCGCTTTGCCG‑TAATCCAGTT‑G‑AATCTGGCGCTGCCAGGTGCTCGCCAGCGAATCCGTAATCGGCATACCGGCGGCGTTTA‑CCATGGCAAACAAA‑CCGATAGCGGAGAATAACAGCCAAAAA‑TT‑CTCGGcaaca                                                                                                                                                                                                                                                       >  1:367794/1‑251 (MQ=255) gCGTATAAGCGGCTAAAAGTGCGGCCAAAATCCAAACGATATTTTGCTCGCCGAAAAAGCCGATCACATTGCCGAACAAAGTCACGCCCACCACAAACGCCAGAGAACCGATTAACCGCGCTTTGCCG‑TAATCCAGTT‑G‑AATCTGGCGCTGCCAGGTGCTCGCCAGCGAATCCGTAATCGGCATACCGGCGGCGTTTA‑CCATGGCAAACAAA‑CCGATAGCGGAGAATAACAGCCAAAAA‑TT‑CTCGGcaaca                                                                                                                                                                                                                                                       >  1:419439/1‑251 (MQ=255) gCGTATAAGCGGCTAAAAGTGCGGCCAAAATCCAAACGATATTTTGCTCGCCGAAAAAGCCGATCACATTGCCGAACAAAGTCACGCCCACCACAAACGCCAGAGAACCGATTAACCGCGCTTTGCCG‑TAATCCAGTT‑G‑AATCTGGCGCTGCCAGGTGCTCGCCAGCGAATCCGTAATCGGCATACCGGCGGCGTTTA‑CCATGGCAAACAAA‑CCGATAGCGGAGAATAACAGCCAAAAA‑TT‑CTCGGcaaca                                                                                                                                                                                                                                                       >  1:678044/1‑251 (MQ=255) gCGTATAAGCGGCTAAAAGTGCGGCCAAAATCCAAACGATATTTTGCTCGCCGAAAAAGCCGATCACATTGCCGAACAAAGTCACGCCCACCACAAACGCCAGAGAACCGATTAACCGCGCTTTGCCG‑TAATCCAGTT‑G‑AATCTGGCGCTGCCAGGTGCTCGCCAGCGAATCCGTAATCGGCATACCGGCGGCGTTTA‑CCATGGCAAACAAA‑CCGATAGCGGAGAATAACAGCCAAAAA‑TT‑CTCGGcaaca                                                                                                                                                                                                                                                       >  1:616861/1‑251 (MQ=255) gCGTATAAGCGGCTAAAAGTGCGGCCAAAATCCAAACGATATTTTGCTCGCCGAAAAAGCCGATCACATTGCCGAACAAAGTCACGCCCACCACAAACGCCAGAGAACCGATTAACCGCGCTTTGCCG‑TAATCCAGTT‑G‑AATCTGGCGCTGCCAGGTGCTCGCCAGCGAATCCGTAATCGGCATACCGGCGGCGTTTA‑CCATGGCAAACAAA‑CCGATAGCGGAGAATAACAGCCAAAAA‑TT‑CTCGGcaaca                                                                                                                                                                                                                                                       >  1:20209/1‑251 (MQ=255)   gTATAAGCGGCTAAAAGTGCGGCCAAAATCCAAACGATATTTTGCTCGCCGAAAAAGCCGATCACATTGCCGAACAAAGTCACGCCCACCACAAACGCCAGAGAACCGATTAACCGCGCTTTGCCG‑TAATCCAGTT‑G‑AATCTGGCGCTGCCAGGTGCTCGCCAGCGAATCCGTAATCGGCATACCGGCGGCGTTTA‑CCATGGCAAACAAA‑CCGATAGCGGAGAATAACAGCCAAAAA‑TT‑CTCGGCAACaaa                                                                                                                                                                                                                                                     >  1:176399/1‑251 (MQ=255)          cGGCTAAAAGTGCGGCCAAAATCCAAACGATATTTTGCTCGCCGAAAAAGCCGATCACATTGCCGAACAAAGTCACGCCCACCACAAACGCCAGAGAACCGATTAACCGCGCTTTGCCG‑TAATCCAGTT‑G‑AATCTGGCGCTGCCAGGTGCTCGCCAGCGAATCCGTAATCGGCATACCGGCGGCGTTTA‑CCATGGCAAACAAA‑CCGATAGCGGAGAATAACAGCCAAAAA‑TT‑CTCGGCAACAAAA‑CTGAGc                                                                                                                                                                                                                                             <  1:375990/251‑1 (MQ=255)                    ggCGGCCAAAATCCAAACGATATTTGGGGCGCCGAAAAAGCCGATAAAATTGCCGAACAAAGCCACGCCCACCACAAACGCCAGAGAACCGATTAACCGCGCTTTGCCG‑TAATCCAGTT‑G‑AATCTGGCGCTGCCAGGTGCTCGCCAGCGAATCCGTAATCGGCATACCGGCGGCGTTTA‑CCATGGCAAACAAA‑CCGATAGCGGAGAATAACAGCCAAAAA‑TT‑CTCGGCAACAAAA‑CGGAGCCCCGCCATa                                                                                                                                                                                                                                    <  1:372334/249‑1 (MQ=255)                      cgcccAAAACCCAACCGTTATTTTGCTCGCCAAAAAAGCCGATCACATTGCCAAAAAAAGTCCCGCCCACCACAAACGCCAGAGAACCGATTAACCGCGCTTTGCCG‑TAATCCAGTT‑G‑AATCTGGCGCTGCCAGGTGCTCGCCAGCGAATCCGTAATCGGCATACCGGCGGCTTTTA‑CCATGGAAAACAAA‑CCGATGGCGGAGAATAACAGCAAAAAA‑TT‑CTCGGCAACAAAA‑CTGACCCCCGCCATAATc                                                                                                                                                                                                                                 <  1:236730/248‑1 (MQ=255)                              aTCCAAACGATATTTTGCTCGCCGAAAAAGCCGATCACATTGCCGAACAAAGTCACGCCCACCACAAACGCCAGAGAACCGATTAACCGCGCTTTGCCG‑TAATCCAGTT‑G‑AATCTGGCGCTGCCAGGTGCTCGCCAGCGAATCCGTAATCGGCATACCGGCGGCGTTTA‑CCATGGCAAACAAA‑CCGATAGCGGAGAATAACAGCCAAAAA‑TT‑CTCGGCAACAAAA‑CTGAGCCCCGCCATAATCAAGGCAc                                                                                                                                                                                                                          >  1:573208/1‑250 (MQ=255)                                  aaccGATATTTTGCCCGCCAAAAAAGCCGATCCCATTGCCGAACAAGGCCCCGCCCACAACAAACGCCAAAGACCCAATTAACCGCCCTTTGCCG‑TAATCC‑TTT‑GAAATCGGGCGCTGCCGGGTGCTCGCCAGCGAACCCGTAATCGGCATCCCGGCGGCGTTTC‑CCA‑GGGAAACAAACCCGATAGCGGAAAATAACAG‑CAAAAA‑TTCCTCGGCAAAAAAC‑CTGAGCCCCGCCATAATCAAGGCACTTGcc                                                                                                                                                                                                                     <  1:665268/248‑1 (MQ=255)                                       atatTTTGCTCGCCGAAAAAGCCGATCACATTGCCGAACAAAGTCACGCCCACCACAAACGCCAGAGAACCGATTAACCGCGCTTTGCCG‑TAATCCAGTT‑G‑AATCTGGCGCTGCCAGGTGCTCGCCAGCGAATCCGTAATCGGCATACCGGCGGCGTTTA‑CCATGGCAAACAAA‑CCGATAGCGGAGAATAACAGCCAAAAA‑TT‑CTCGGCAACAAAA‑CTGAGCCCCGCCATAATCAAGGCACTTGCCAgcg                                                                                                                                                                                                                 >  1:429208/1‑250 (MQ=255)                                       atatTTTGCTCGCCGAAAAAGCCGATCACATTGCCGAACAAAGTCACGCCCACCACAAACGCCAGAGAACCGATTAACCGCGCTTTGCCG‑TAATCCAGTT‑G‑AATCTGGCGCTGCCAGGTGCTCGCCAGCGAATCCGTAATCGGCATACCGGCGGCGTTTA‑CCATGGCAAACAAA‑CCGATAGCGGAGAATAACAGCCAAAAA‑TT‑CTCGGCAACAAAA‑CTGAGCCCCGCCATAATCAAGGCACTTGCCAgcg                                                                                                                                                                                                                 >  1:272322/1‑250 (MQ=255)                                               ctgcccGAAAAACCCGATCACATTGCGAAAAAAAGTCCCGCCCACCAAAAACGCCAGAGAACGGATTACCCGCGCTTTGCCG‑TAACCCAGTT‑G‑AATCGGGCGCTGCCAGGTGCTCGCCAGCGAATCCGTAATCGGCATCCCGGCGGCTTTTA‑CCATGGCAAAAAAC‑CCGAGAGCGGAGAATAACAGCAAAAAA‑TT‑CTCGGCAACAAAA‑CTGACCCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAg                                                                                                                                                                                                         <  1:105716/246‑1 (MQ=255)                                                             cgaccccagtcccgaaaaattccACGCCCCCCACAACCGCCAAAAAACCAATTACCCGCG‑TTTTCCGATAAACAAGTG‑A‑AATCTGGCGCCGCCAGGTCCCAGCCAGCGAATCCGAAACCGGAATCCGGGCGGC‑TTTACCCAGGACAAAAAAC‑CCGATGGGGGAGAAAAACAGCAAAAAT‑TT‑CGCGGCAAAAAAA‑CTGACCCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATc                                                                                                                                                                                          <  1:289456/229‑1 (MQ=255)                                                             cGATCACATTGCCGAACAAAGCCCCGCCCACCACAAACGCCAGAGAACCGATTACCCGCGCTTTGCCG‑TAATCCAGTT‑G‑AATCTGGCGCTGCCAGGTGCTCCCCAGCGAATCCGTAATCGGCATCCCGGCGGCGTTTA‑CCATGGCAAACAAA‑CCGATAGCGGAAAATAACAGCAAAAAA‑TT‑CTCGGCAAAAAAC‑CTGAGCCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATc                                                                                                                                                                                          <  1:168007/251‑1 (MQ=255)                                                             cGATCACATTGCCAAACAAAGTCACGCCAACCACAAACGCCAGAGACCCAATTACCCGCGCTTTGCCG‑TAATCCAGTT‑G‑AATCGGGCGCTGCCAGGTGCTCGCCAGCAAATCCGTAATCGGCATCCCGGCGGCTTTTC‑CCATGGCAAACAAA‑CCGATAGCGGAGAAAAACAGCAAAAAA‑TT‑CTCGGCAAAAAAA‑CTGAGCCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATc                                                                                                                                                                                          <  1:819875/251‑1 (MQ=255)                                                                ccactttGCCAAACAAACTCACACCCCCCACAACCGCCAGAGACCCGATTACCCGCCTTTTGCCT‑TATCCCAGTT‑G‑AATCTGGCCCTGCCAGTTCCTCGCCAGCAACCCCGTAACCGTCATCCCGGCGGCTTTTA‑CCATGGAAAAAAAC‑CCGATAGCGGAAAAAAACA‑CCAAAAATTT‑CTCGCCAAAAAAA‑CGGCCCCCCGACATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGc                                                                                                                                                                                       <  1:787422/246‑1 (MQ=255)                                                                 cacaTTGCCGAACAAAGTCACGCCCACCACAAACGCCAGAGAACCGATTAACCGCGCTTTGCCG‑TAATCCAGTT‑G‑AATCTGGCGCTGCCAGGTGCTCGCCAGCGAATCCGTAATCGGCATACCGGCGGCGTTTA‑CCATGGCAAACAAA‑CCGATAGCGGAGAATAACAGCCAAAAA‑TT‑CTCGGCAACAAAA‑CTGAGCCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAg                                                                                                                                                                                        >  1:159130/1‑249 (MQ=255)                                                                     tggccaaaCAAAGTCACCCCCCCCACAAACGCAAGAGACCCGATAACCCGCGCTTGGCCG‑TAATCCAGTT‑G‑AATCGGGCGCTGCCAGGTGCGCGCCAGCGAATCCGTAATCGGCATACCGGCGGCGTTTA‑CCATGTCAAACAAA‑CCGATAGGGGAGAATAACAGCAAAAAA‑TC‑TTCGGGAAAAAAT‑CGGAGTCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCTGCGa                                                                                                                                                                                  <  1:574092/245‑1 (MQ=255)                                                                       gCCGAACAAAGTCACGCCCACCACAAACGCCAGAGAACCGATTAACCGCGCTTTGCCG‑TAATCCAGTT‑G‑AATCTGGCGCTGCCAGGTGCTCGCCAGCGAATCCGTAATCGGCATACCGGCGGCGTTTA‑CCATGGCAAACAAA‑CCGATAGCGGAGAATAACAGCCAAAAA‑TT‑CTCGGCAACAAAA‑CTGAGCCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCTGCGACg                                                                                                                                                                                >  1:516521/1‑251 (MQ=255)                                                                               aaGTCCCGCCCCCCACAACCGCCAGAGAACCGATAACCCGCGCTTTGCCG‑TAATCCAGTT‑G‑AATCTGGCGCTCCCAGGTGCCCCCCAGCGAATCCGTAATCGGCATCCCGGCGGCTTTTC‑CCAGGGAAAACAAC‑CCGATAGCGGAAAATAACCGCAAAAAA‑TT‑CTC‑GCAAAAAAACCTGAGCCCCGCCATAATCAAGGCACTTGCCAGCGCCAATa                                                                                                                                                                                                           <  1:308426/216‑1 (MQ=255)                                                                               aaGTCACGCCCACCACAAACGCCAGAGAACCGATTAACCGCGCTTTGCCG‑TAATCCAGTT‑G‑AATCTGGCGCTGCCAGGTGCTCGCCAGCGAATCCGTAATCGGCATACCGGCGGCGTTTA‑CCATGGCAAACAAA‑CCGATAGCGGAGAATAACAGCCAAAAA‑TT‑CTCGGCAACAAAA‑CTGAGCCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCTGCGACGCCCGTtta                                                                                                                                                                        >  1:381144/1‑251 (MQ=255)                                                                                      gCCCACCACAAACGCCAGAGAACCGATTAACCGCGCTTTGCCG‑TAATCCAGTT‑G‑AATCTGGCGCTGCCAGGTGCTCGCCAGCGAATCCGTAATCGGCATACCGGCGGCGTTTA‑CCATGGCAAACAAA‑CCGATAGCGGAGAATAACAGCCAAAAA‑TT‑CTCGGCAACAAAA‑CTGAGCCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCTGCGACGCCCGTTTAATTaaa                                                                                                                                                                  >  1:739427/1‑250 (MQ=255)                                                                                      cccccccaaaacccccagaaacccGATTACCCGCGCTTCGCCG‑AAATCCAGTT‑G‑AATCGGGCGCTGCCGGGTGCCCGCCAGCGAACCCGTAACCGGCATACCGGCGGCTTTTC‑CCAGGGAAAAAAAA‑CCGATAGGGGAAAAAAACAGCAAAAAA‑TT‑CCCGGCAAAAAAC‑CGGAGCCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCTGCGACGCCCGTTTAATTAAAc                                                                                                                                                                 <  1:466519/229‑1 (MQ=255)                                                                                                    ccAGAGAACCGATTAACCGCGCTTTGCCG‑TAATCCAGTT‑G‑AATCTGGCGCTGCCAGGTGCTCGCCAGCGAATCCGTAATCGGCATACCGGCGGCGTTTA‑CCATGGCAAACAAA‑CCGATAGCGGAGAATAACAGCCAAAAA‑TT‑CTCGGCAACAAAA‑CTGAGCCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCTGCGACGCCCGTTTAATTAAACCGGAGAAGAACaaa                                                                                                                                                   >  1:222321/1‑251 (MQ=255)                                                                                                         gaACCGATTAACCGCGCTTTGCCG‑TAATCCAGTT‑G‑AATCTGGCGCTGCCAGGTGCTCGCCAGCGAATCCGTAATCGGCATACCGGCGGCGTTTA‑CCATGGCAAACAAA‑CCGATAGCGGAGAATAACAGCCAAAAA‑TT‑CTCGGCAACAAAA‑CTg                                                                                                                                                                                                                                                >  1:186055/1‑153 (MQ=255)                                                                                                                 tacccGCGCTTTCCCG‑TAATCCATTT‑G‑ATTCGGGCGCTGCCAGGTGCTCGCCAGCGAACCCGTAACCGGCATACCGGCGGCTTTTA‑CCATGGCAAAAAAA‑CCGATAGCGGAGAATAACAACAAAAAA‑TT‑CTCGGCAAAAAAA‑CTGAGCCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCTGCGACGCCCGTTTAATTAAACCGGAGAAGAACAAACCGCCCACAAAAc                                                                                                                                      <  1:649347/248‑1 (MQ=255)                                                                                                                 tAACCGCGCTTTGCCG‑TAATCCAGTT‑G‑AATCTGGCGCTGCCAGGTGCTCGCCAGCGAATCCGTAATCGGCATACCGGCGGCGTTTA‑CCATGGCAAACAAA‑CCGATAGCGGAGAATAACAGCCAAAAA‑TT‑CTCGGCAACAAAA‑CTGAGCCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCTGCGACGCCCGTTTAATTAAACCGGAGAAGAACAAACCGCCCACaaaa                                                                                                                                       >  1:200335/1‑250 (MQ=255)                                                                                                                 aaaCCGCGCTTTGCCG‑TAATCCAGTT‑G‑AATCTGGCGCTGCCAGGTGCTCGCCAGCGAATCCGTAATCGGCATACCGGCGGCGTTTA‑CCATGGAAAACAAA‑CCGATAGCGGAGAATAACAGCCAAAAA‑TT‑CTCGGAAAAAAAC‑CTGGGCCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCTGCGACGCCCGTTTAATTAAACCGGAGAAGAACAAACCGCCCACAAAAc                                                                                                                                      <  1:569525/250‑1 (MQ=255)                                                                                                                         cttggCCG‑TAATCCGGTG‑G‑AATCGGGCGCTGCCAGGGGCGCGCCAGCGAATCCGAAATCGGCATACCGGCGGCTTTTA‑CCATGAAAAAAAAA‑CCGATAGCGGAAAAAAACAGCAAAAAA‑TT‑CGCGGCAAAAAAA‑CTGAGCCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCTGCGACGCCCGTTTAATTAAACCGGAGAAGAACAAACCGCCCACAAAACGGAacac                                                                                                                               <  1:483609/246‑1 (MQ=255)                                                                                                                                   aaaccAGTTAG‑AATCAGGCGCAGCGGGGTACTCGCGAGAGAATCCGAAATCGGAAGCCCGGCGTCTTTAA‑CCATGGCAAACAAC‑CCGATGGAGAAGAATAAAAGCAAAAAT‑TT‑CTCGACAACAAAA‑CGGACCCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCTGCGACGCCCGTTTAATTAAACCGGAGAAGAACAAACCGCCCACAAAACGGAACACATAAGCAc                                                                                                                       <  1:736193/247‑1 (MQ=255)                                                                                                                                           t‑G‑AATCTGGCGCTGCCAGGTGCTCGCCAGCGAACCCGTAATCGGCATCCCGGCGGCTTTTC‑CCAGGGAAAACAAC‑CCGAAAGCGGAAAATAACAGCCAAAAT‑TT‑CTCGCCAAAAAAC‑CTGCCCCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCTGCGACGCCCGTTTAATTAAACCGGAGAAGAACAAACCGCCCACAAAACGGAACACATAAGCACTCGCCAGCAc                                                                                                             <  1:110805/251‑1 (MQ=255)                                                                                                                                           t‑G‑AATCGGGCGCTGCCAGGTGCGCGCCAGCGAAACGGTAATCGGCATACCGGCGGCTTTTA‑CCATGACAAACAAC‑CCTACAGCGAAAAACACCAGCAAAAAA‑TT‑CTCGGCAAAAAAA‑CTGAGCCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCTGCGACGCCCGTTTAATTAAACCGGAGAAGAACAAACCGCCCACAAAACGGAACACATAAGCACTCGCCAGCAc                                                                                                             <  1:612691/251‑1 (MQ=255)                                                                                                                                           t‑G‑AATCGGGCGCTGCCAGGTGCCCGCCAGCGAATCCGAAACCGGCATACCGGCGGCGTTTA‑CAAGGGAAAACAAA‑CCGATAGCGGAAAAAAACAGCAAAAAA‑TT‑CTCGGAAACAAAA‑CGGGGCCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCTGCGACGCCCGTTTAATTAAACCGGAGAAGAACAAACCGCCCACAAAACGGAACACATAAGCACTCGCCAGCAc                                                                                                             <  1:150065/251‑1 (MQ=255)                                                                                                                                             taaatcgggagtcacaaGGTGCTCGACAGAAAACCCGTAACCGACATACCGGGGGGGTTTC‑CCTTGCCAAAAAAA‑CCGATAGCGGAAAATAAAACAAAAAAA‑TT‑CCCGAAAAAAAAC‑CTGAGCCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCTGCGACGCCCGTTTAATTAAACCGGAGAAGAACAAACCGCCCACAAAACGGAACACATAAGCACTCGCCAGCAc                                                                                                             <  1:445501/235‑1 (MQ=255)                                                                                                                                             g‑AATCTGGCGCTGCCAGGTCCCCGCCAGCGAACCCGTAATCGGCATCCCGGCGGCGTTTA‑CCAGGGCAAACAAC‑CCGATAGCGGAAAATAACAGCCAAAAA‑TT‑CTCGGCAAAAAAA‑CTGAGCCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCTGCGACGCCCGTTTAATTAAACCGGAGAAGAACAAACCGCCCACAAAACGGAACACATAAGCACTCGCCAGCAc                                                                                                             <  1:311356/250‑1 (MQ=255)                                                                                                                                              gagtgtGGCGCGGCGAGTTGCTCGCCAGCAAATCCGTAAACGGCATACGGGCGGCGTTTC‑CCATGGCAAACAAT‑CCGATGGCGGAGAAAAGCAGCAAAAAA‑TT‑CCCGGCAAAAAAG‑CTGACCCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCTGCGACGCCCGTTTAATTAAACCGGAGAAGAACAAACCGCCCACAAAACGGAACACATAAGCACTCGCCAGCAc                                                                                                             <  1:35665/245‑1 (MQ=255)                                                                                                                                              gaaccgggcgcccccagtcgCTCGCCAGCAACTCCGTAACCGGCATCCCGGCGGCTTTTC‑CCATGACAAACAAC‑CCAATAGCGGAAAAAAACACCCAAAAA‑TT‑CTCGGAAAAAAAA‑CTGAGCCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCTGCGACGCCCGTTTAATTAAACCGGAGAAGAACAAACCGCCCACAAAACGGAACACATAAGCACTCGCCAGCAc                                                                                                             <  1:270494/231‑1 (MQ=255)                                                                                                                                                  cTGGCGCTGCCAGTTGCCCGCCAGCGACTCCTTAATCGGCATCCCGGCGGTTTTTC‑CCATGGCAAACAAC‑CCGATAGCGGAGAATAACAGCAAAAAA‑TT‑CTCGGCAAAAAAA‑CTGACCCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCTGCGACGCCCGTTTAATTAAACCGGAGAAGAACAAACCGCCCACAAAACGGAACACATAAGCACTCGCCAGCACCATGc                                                                                                        <  1:365508/251‑1 (MQ=255)                                                                                                                                                        cggCCAGGTGCTCGCCAGAGAATCCGTAATCGGCATACGGGCGGCTTTTC‑CAAGGGAAAAAAAA‑CCGAGAGCGGAAAATAACAGCAAAAAA‑TT‑CGGGGCAACAAAA‑CGGAGCCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCTGCGACGCCCGTTTAATTAAACCGGAGAAGAACAAACCGCCCACAAAACGGAACACATAAGCACTCGCCAGCACCATGCCGATc                                                                                                   <  1:142193/248‑1 (MQ=255)                                                                                                                                                          gCCAGGTGCTCGCCAGCGAATCCGTAATCGGCATACCGGCGGCGTTTA‑CCATGGCAAACAAA‑CCGATAGCGGAGAATAACAGCCAAAAA‑TT‑CTCGGCAACAAAA‑CTGAGCCCCGCCATAATc                                                                                                                                                                                                                                 >  1:124829/1‑122 (MQ=255)                                                                                                                                                          gCCAGGTGCTCGCCAGCGAATCCGTAATCGGCATACCGGCGGCGTTTA‑CCATGGCAAACAAA‑CCGATAGCGGAGAATAACAGCCAAAAA‑TT‑CTCGGCAACAAAA‑CTGAGCCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCTGCGACGCCCGTTTAATTAAACCGGAGAAGAACAAACCGCCCACAAAACGGAACACATAAGCACTCGCCAGCACCATGCCGATCAtt                                                                                                >  1:222047/1‑251 (MQ=255)                                                                                                                                                            cgggggcccGCCAGCGAATCCGTAACCGGCATACCGGCGGCGTTTA‑CCAGGGAAAAAAAA‑CCGATAGCGGAAAAAAACACCAAAAAA‑TT‑CTCGCCACAAAAA‑CTGAGCCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCTGCGACGCCCGTTTAATTAAACCGGAGAAGAACAAACCGCCCACAAAACGGAACACATAAGCACTCGCCAGCACCATGCCGATCATTTc                                                                                              <  1:266609/243‑1 (MQ=255)                                                                                                                                                               gTGCTCGCCAGCGAATCCGTAATCGGCATACCGGCGGCGTTTA‑CCATGGCAAACAAA‑CCGATAGCGGAGAATAACAGCCAAAAA‑TT‑CTCGGCAACAAAA‑CTGAGCCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAg                                                                                                                                                                                                         >  1:253279/1‑141 (MQ=255)                                                                                                                                                               gTGCTCGCCAGCGAATCCGTAACCGGCATCCCGGCGGCTTTTC‑CCATGGCAAACAAA‑CCGATAGCGGAAAATAACACCAAAAAA‑TT‑CTCGGCAAAAAAA‑CTGAGCCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCTGCGACGCCCGTTTAATTAAACCGGAGAAGAACAAACCGCCCACAAAACGGAACACATAAGCACTCGCCAGCACCATGCCGATCATTTc                                                                                              <  1:130276/248‑1 (MQ=255)                                                                                                                                                                      ccAGCGAATCCGTAATCGGCATACCGGCGGCGTTTA‑CCATGGCAAACAAA‑CCGATAGCGGAGAATAACAGCCAAAAA‑TT‑CTCGGCAACAAAA‑CTGAGCCCCGCCa                                                                                                                                                                                                                                      >  1:479566/1‑105 (MQ=255)                                                                                                                                                                       cAGCGAATCCGTAATCGGCATACCGGCGGCGTTTA‑CCATGGCAAACAAA‑CCGATAGCGGAGAATAACAGCCAAAAA‑TT‑CTCGGCAACAAAA‑CTGAGCCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCTGCGACGCCCGTTTAATTAAACCGGAGAAGAACAAACCGCCCACAAAACGGAACACATAAGCAc                                                                                                                       >  1:450406/1‑215 (MQ=255)                                                                                                                                                                            aaTCCGTAATCGGCATACCGGCGGCGTTTA‑CCATGGCAAACAAA‑CCGATAGCGGAGAATAACAGCCAAAAA‑TT‑CTCGGCAACAAAA‑CTGAGCCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCTGCGACGCCCGTTTAATTAAACCGGAGAAGAACAAACCGCCCACAAAACGGAACACATAAGCACTCGCCAGCACCATGc                                                                                                        >  1:413853/1‑225 (MQ=255)                                                                                                                                                                             aTCCGTAATCGGCATACCGGCGGCGTTTA‑CCATGGCAAACAAA‑CCGATAGCGGAGAATAACAGCCAAAAA‑TT‑CTCGGCAACAAAA‑CTGAGCCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCTGCGACGCCCGTTTAATTAAACCGGAGAAGAACAAACCGCCCACAAAACGGAACACATAAGCACTCGCCAGCACCATGCCGATCATTTCCTCGCCGTATTGTTggg                                                                             >  1:201879/1‑251 (MQ=255)                                                                                                                                                                                        gCATACCGGCGGCGTTTA‑CCATGGCAAACAAA‑CCGATAGCGGAGAATAACAGCCAAAAA‑TT‑CTCGGCAACAAAA‑CTGAGCCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCTGCGACGCCCGTTTAATTAAACCGGAGAAGAACAAACCGCCCACAAAACGGAACACATAAGCACTCGCCAGCACCATGCCGATCATTTCCTCGCCGTATTGTTGGGATTTCATCCaa                                                                  >  1:830820/1‑251 (MQ=255)                                                                                                                                                                                           tACCGGCGGCGTTTA‑CCATGGCAAACAAA‑CCGATAGCGGAGAATAACAGCCAAAAA‑TT‑CTCGGCAACAAAA‑CTGAGCCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCTGCGACGCCCGTTTAATTAAACCGGAGAAGAACAAACCGCCCACAAAACGGAACACATAAGCACTCGCCAGCACCATGCCGATCATTTCCTCGCCGTATTGTTGGGATTTCATCCAAACg                                                               >  1:217223/1‑251 (MQ=255)                                                                                                                                                                                                                gaaaaaaaa‑cAGATAGGGGAAAAAAACAGCAAAAAA‑TT‑CTCGGAAAAAAAA‑CGGAGCCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCTGCGACGCCCGTTTAATTAAACCGGAGAAGAACAAACCGCCCACAAAACGGAACACATAAGCACTCGCCAGCACCATGCCGATCATTTCCTCGCCGTATTGTTGGGATTTCATCCAAACGGGAAAAAACGGCACGAATa                                            <  1:93330/244‑1 (MQ=255)                                                                                                                                                                                                                 caaacaaa‑CCGATAGCGGAGAATAACAGCCAAAAA‑TT‑CTCGGCAACAAAA‑CTGAGCCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCTGCGACGCCCGTTTAATTAAACCGGAGAAGAACAAACCGCCCACAAAACGGAACACATAAGCACTCGCCAGCACCATGCCGATCATTTCCTCGCCGTATTGTTGGGATTTCATCCAAACGGGAAAAAACGGCACGAATACg                                          >  1:306577/1‑251 (MQ=255)                                                                                                                                                                                                                   aacaaa‑cCGATAGCGGAGAATAACAGCCAAAAA‑TT‑CTCGGCAACAAAA‑CTGAGCCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCTGCGACGCCCGTTTAATTAAACCGGAGAAGAACAAACCGCCCACAAAACGGAACACATAAGCACTCGCCAGCACCATGCCGATCATTTCCTCGCCGTATTGTTGGGATTTCATCCAAACGGGAAAAAACGGCACGAATACGc                                         >  1:258201/1‑250 (MQ=255)                                                                                                                                                                                                                     caaa‑cCGATAGCGGAGAATAACAGCCAAAAA‑TT‑CTCGGCAACAAAA‑CTGAGCCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCTGCGACGCCCGTTTAATTAAACCGGAGAAGAACAAACCGCCCACAAAACGGAACACATAAGCACTCGCCAGCACCATGCCGATCATTTCCTCGCCGTATTGTTGGGATTTCATCCAAACGGGAAAAAACGGCACGAATACGCCAt                                      >  1:796873/1‑251 (MQ=255)                                                                                                                                                                                                                         cccgatagggaaaaaTAACA‑CCAAAAA‑TTCCGCGGAAAAAAAC‑CTGAGCCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCTGCGACGCCCGTTTAATTAAACCGGAGAAGAACAAACCGCCCACAAAACGGAACACATAAGCACTCGCCAGCACCATGCCGATCATTTCCTCGCCGTATTGTTGGGATTTCATCCAAACGGGAAAAAACGGCACGAATACGCCATAAg                                   <  1:731476/238‑1 (MQ=255)                                                                                                                                                                                                                             aTAGGGGAAAATAACCGCAAAAAA‑TC‑CTGGGCAAAAAAC‑CGGAGCCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCTGCGACGCCCGTTTAATTAAACCGGAGAAGAACAAACCGCCCACAAAACGGAACACATAAGCACTCGCCAGCACCATGCCGATCATTTCCTCGCCGTATTGTTGGGATTTCATCCAAACGGGAAAAAACGGCACGAATACGCCATAAgcgc                                <  1:174356/250‑1 (MQ=255)                                                                                                                                                                                                                                 cggaaaaaaaCAGCAAAAAA‑TT‑CTCGGCAAAAAAA‑CGGAGCCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCTGCGa                                                                                                                                                                                  <  1:243072/92‑1 (MQ=255)                                                                                                                                                                                                                                  ggaaaaaaaCAGCCAAAAT‑TT‑CTCGGCAACAAAC‑CGGACCCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCTGCGACGCCCGTTTAATTAAACCGGAGAAGAACAAACCGCCCACAAAACGGAACACATAAGCACTCGCCAGCACCATGCCGATCATTTCCTCGCCGTATTGTTGGGATTTCATCCAAACGGGAAAAAACGGCACGAATACGCCATAAGCGCagtag                           <  1:640227/243‑1 (MQ=255)                                                                                                                                                                                                                                  ggAGAAAAACAGCAAAAAA‑TT‑CTCGGCAACAAAA‑CGGAGCCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCTGCGACGCCCGTTTAATTAAACCGGAGAAGAACAAACCGCCCACAAAACGGAACACATAAGCACTCGCCAGCACCATGCCGATCATTTCCTCGCCGTATTGTTGGGATTTCATCCAAACGGGAAAAAACGGCACGAATACGCCATAAGCGCagtag                           <  1:320734/250‑1 (MQ=255)                                                                                                                                                                                                                                           cAGCCAAAAA‑TT‑CTCGGCAACAAAA‑CTGAGCCCCGCCATAATCAAGGCACTTGTCAGCGCCAATAAGCGCAAGGAATTAATCAGCTGCGACGCCCGTTTAATTAAACCGGAGAAGAACAAACCGCCCACAAAACGGAACACATAAGCACTCGCCAGCACCATGCCGATCATTTCCTCGCCGTATTGTTGGGATTTCATCCAAACGGGAAAAAACGGCACGAATACGCCATAAGCGCAGTAGTAGCCGaaaa                 >  1:424962/1‑251 (MQ=255)                                                                                                                                                                                                                                                             gCAAAAAAC‑CTGACCCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCTGCGACGCCCGTTTAATTAACCCGGAGAAGAACAAACCGCCCACAAAACGGAACACATAAGCACTCGCCAGCACCATGCCGATCATTTCCTCGCCGTATTGTTGGGATTTCATCCAAACGGGAAAAAACGGCACGAATACGCCATAAGCGCAGTAGTAGCCGAAAAAGCTCAAGGCGAGcc  <  1:611421/250‑1 (MQ=255)                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                       GCGTATAAGCGGCTAAAAGTGCGGCCAAAATCCAAACGATATTTTGCTCGCCGAAAAAGCCGATCACATTGCCGAACAAAGTCACGCCCACCACAAACGCCAGAGAACCGATTAACCGCGCTTTGCCG‑TAATCCAGTT‑G‑AATCTGGCGCTGCCAGGTGCTCGCCAGCGAATCCGTAATCGGCATACCGGCGGCGTTTA‑CCATGGCAAACAAA‑CCGATAGCGGAGAATAACAGCCAAAAA‑TT‑CTCGGCAACAAAA‑CTGAGCCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCTGCGACGCCCGTTTAATTAAACCGGAGAAGAACAAACCGCCCACAAAACGGAACACATAAGCACTCGCCAGCACCATGCCGATCATTTCCTCGCCGTATTGTTGGGATTTCATCCAAACGGGAAAAAACGGCACGAATACGCCATAAGCGCAGTAGTAGCCGAAAAAGCTCAAGGCGAGCC  >  1/2137857‑2138351

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑