Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*11,999,2510GA47.2% 75.4 / 32.1 72intergenic (+149875/‑741)unknown/unknowntRNA‑Leu‑CAA/tRNA‑Asp‑GTC
Reads supporting (aligned to +/- strand):  ref base G (31/7);  new base A (0/34);  total (31/41)
Fisher's exact test for biased strand distribution p-value = 6.46e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01
Rejected as consensus: Frequency below/above cutoff threshold.

GATACCGCCCGCACCGGGGTTGCCGAGGCAGGATCCGTCGGTAAAAATTTCGATTTGTTTGCGCATATTGACTACTAATTTATTTCAAATGAGGCGATAATACCCG‑AAATTTTT‑GCGTATAAAAAGTAGCGTTATGAGTATTCAAATTAACCCGAATCGCCAAATTGT‑ACTGGATACGGAAA‑CCACC‑GGTATGAACCAGTTAGGCGCCCATTATGAAGGTCA‑TTGCATCATT‑GAAAT‑CGGTGCGGTGGAAATGATTAACCGCCGTTATACCGGCAATAATTTTCACATTTATATTAAGCCGAATCGTCCGGTGGATCCCGATGCTATTAAGGTACACGGTATCACCGACGAAATGCTGGCGGACAAACCGGATTTCAGTCAGGCCGCCAATGAATTCATCGAATATATCAAAGGCGCCGAGTTGCTTATTCACAACGCGCCCTTCGACGTGGGCTTCATGGATTATGAATTCGCCAAGCTGAACCTGCCGATTAA  >  1/1999004‑1999498
                                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                       
gATACCGCCCGCACCGGGGTTGCCGAGGCAGGATCCGTCGGTAAAAATTTCGATTTGTTTGCGCATATTGACTACTAATTTATTTCAAATGAGGCGATAATACCCG‑AAATTTTT‑GCGTATAAAAAGTAGCGTTATGAGTATTCAAATTAACCCGAATCGCCAAATTGT‑ACTGGATACGGAAA‑CCACC‑GGTATGAAACAGTTAGGCGCCCATTATGAAGGTCA‑TTGCATCATT‑GAAAT‑CGGTGCGGTGGaaa                                                                                                                                                                                                                                                      >  1:62469/1‑251 (MQ=255)
           cACCGGGGTTGCCGAGGCAGGATCCGTCGGTAAAAATTTCGATTTGTTTGCGCATATTGACTACTAATTTATTTCAAATGAGGCGATAATACCCG‑AAATTTTT‑GCGTATAAAAAGTAGCGTTATGAGTATTCAAATTAACCCGAATCGCCAAATTGT‑ACTGGATACGGAAA‑CCACC‑GGTATGAACCAGTTAGGCGCCCATTATGAAGGTCA‑TTGCATCATT‑GAAAT‑CGGTGCGGTGGAAATGATTAAccg                                                                                                                                                                                                                                            >  1:564515/1‑250 (MQ=255)
                                     tcgttAAAAATTTCGTTTTGTTTGCGAATATTGACTACTATTTTTTTTCAAATGAGGCGAAAATCCCCA‑AATTTTTT‑GCGTATAAAAAGTCGCGTTATGAGTTTAAAAATTACCCCGAACCGCAAATTTGT‑ACGGGATACGAAAC‑CCACC‑GGTATGACCCATTGGGGCCCCCATTATGAAGTTCT‑TTGCATCATT‑GAAAG‑CGGTGCGGTGAAAATAATTAACCGCCGTTATACCGGCAATAATTTTcaca                                                                                                                                                                                                                  <  1:482386/246‑1 (MQ=255)
                                             attttCTATTTGTTTGCGCATATTGACTACTAATTTATTACAAATGAGGCAATAATCCCCA‑AAATTTGT‑GCGAATAAAAAGTAGCGTTATGATTTTTCAAATTACCCCGAATCGCCAAATTTT‑ACTGGATACGAAAC‑CCCCC‑GGTATGACCCAGTTAGGCGCCCTTTATGAAGTTCT‑TTGCATCATT‑GAATT‑CGGTGCGGTGAAAATGTTAAACCGCCGTTATACCGGCAATAATTTTCACATTTATATTa                                                                                                                                                                                                         <  1:704622/249‑1 (MQ=255)
                                                ttcgttttttttgcaaatatTGACTACTAATTTATTTAAAAGGGGGCTATAATCCCCA‑AAATTTTT‑GCGTAAAAAAGGTAGCGTAATGATTATT‑AAATTTACCCCGATCCCCAAATTTTAACTGAATACGAAAC‑CCCCC‑TGTATTATCTAGTTGGCCGCCCATTATGTCGTTCA‑TTGCATAATT‑AAAAT‑CGTTGGGGTGAAAGTGATTAACCGCCGTTATACCGGCAATAATTTTCACATTTATATTAAGc                                                                                                                                                                                                      <  1:803558/235‑1 (MQ=255)
                                                ttCGATTTTTTTGCGCATATTGACTATTATTTTATTACAAAGGAGGCAATAATCCCAA‑AAATTTTT‑GCGTAAAAAAGGTAGCGTTATGAGTATTAAAATTACCCCAAACCGCAAAATTGT‑ACTGGATACGGAAA‑CCCCC‑GTTATGACCCAGTTAGGCGCCCATTATGAAGTTCT‑TTGCATCATT‑GAAAT‑CGGTGGGGTGAAAATGATTAACCGCCGTTATACCGGCAATAATTTTCACATTTATATTaggc                                                                                                                                                                                                      <  1:512226/251‑4 (MQ=255)
                                                     tttgtttgCGAATATTGCCTATTAATTTATTTCAAATGGGGCGATAATCCCCA‑AAATTTTT‑GCTTATAAAAAGTAGCTTTATGAGTATTCAAATTACCCCAAATCGCAAAATTTT‑ACTGGATGCGAAAA‑CCCCC‑GGTATGACCCAGTTAGGCGCCCATTATGAAGGTCA‑TTCCATCATT‑GAAAT‑CGGGGGGGGGAAAATAATTAACCGCCGTTATACCGGCAATAATTTTCACATTTATATTAAGCCGAAt                                                                                                                                                                                                 <  1:437707/251‑1 (MQ=255)
                                                     tttgtttgCGAATATTGACTACTAATTTATTTAAAAGGAGGCGAAAAT‑CCCGAAAATTTTT‑GCGTATAAAAAGGAGCGTTATGAGTATTCAAATTAACCCGAATCGCAAAATTGT‑CCGGAATGGGGAAC‑CCACC‑GGTATAACCCAGTTAGGCCCCAATTATGAGGTTAA‑TTGCATCATT‑AAAAT‑CGGTGCGGTGAAAATGATTAACCGCCGTTATACCGGCAATAATTTTCACATTTATATTAAGCCGAAt                                                                                                                                                                                                 <  1:471552/251‑1 (MQ=255)
                                                        gtttgCGCATATTGACTACTAATTTATTTCAAATGAGGCGATAATACCCG‑AAATTTTT‑GCGTATAAAAAGTAGCGTTATGAGTATTCAAATTAACCCGAATCGCCAAATTGT‑ACTGGATACGGAAA‑CCACC‑GGTATGAACCAGTTAGGCGCCCATTATGAAGGTCA‑TTGCATCATT‑GAAAT‑CGGTGCGGTGGAAATGAtt                                                                                                                                                                                                                                                 <  1:222205/200‑1 (MQ=255)
                                                               cATATTGACTACTAATTTATTTCAAATGAGGCGATAATACCCG‑AAATTTTT‑GCGTATAAAAAGTAGCGTTATGAGTATTCAAATTAACCCGAATCGCCAAATTGT‑ACTGGATACGGAAA‑CCACC‑GGTATGAACCAGTTAGGCGCCCATTATGAAGGTCA‑TTGCATCATT‑GAAAT‑CGGTGCGGTgg                                                                                                                                                                                                                                                         >  1:549004/1‑185 (MQ=255)
                                                               cATATTGACTACTAATTTATTTAAAAGGAGGCAATAATCCCCG‑AAATTTTG‑GCGTATAAAAAGTAGTTTTATGAGTATTAAAATTACCCCGAATCGCCAAATTGT‑ACTGGATACGGAAA‑CCACC‑GTTATGAACCAGTTAGGCGCCTTTTATGAAGGTCT‑TTGCATCATT‑GAAAT‑CGGTGCGGGGGAAATGATTAACCGCCGTTATACCGGCAATAATTTTCACATTTATATTAAGCCGAATCGTCCGGTgg                                                                                                                                                                                       <  1:70072/251‑1 (MQ=255)
                                                               aatatTGACTACTAATTTATTTAAAATGAGGCAATAATACCGA‑AAATTTTT‑GGGTAAAAAAAGTGGCGTTATGAGTATTAAAATTACCCCGAATCGCAAAATTGT‑ACGGGATACGAAAA‑CCCCC‑GGTATGAACCAGTTAGGCGCCCATTATGAAGGTCA‑TTGCATAATT‑AAAAT‑CGGGGGGGTGGAAATGATTAACCGCCGTTATACCGGCAATAATTTTCACATTTATATTAAGCCGAATCGTCCGGTgg                                                                                                                                                                                       <  1:382146/250‑1 (MQ=255)
                                                                    taactacaAATTTATTTAAAATGAGGGTATAATCCCCG‑AATTTTTT‑GCGTAAAAAAAGGAGCGTTATGATTATTAAAATTACCCCAAACCCCAAAATTGT‑ACTGGATACGAAAC‑CCACC‑GGTATAACCCAGTAAGGCCCCCATTATGAAGGTCT‑TTGCATCATT‑GAAAC‑CGGTGGGGGGAAAATGATTAACCGCCGTTATACCGGCAATAATTTTCACATTTATATTAAGCCGAATCGTCCGGTGGATccc                                                                                                                                                                                  <  1:47625/249‑1 (MQ=255)
                                                                       ctactaATTTATTTCAAATGAGGCGATAATACCCG‑AAATTTTT‑GCGTATAAAAAGTAGCGTTATGAGTATTCAAATTAACCCGAATCGCCAAATTGT‑ACTGGATACGGAAA‑CCACC‑GGTATGAACCAGTTAGGCGCCCATTATGAAGGTCA‑TTGCATCATT‑GAAAT‑CGGTGCGGTGGAAATGATTAACCGCCGTTATACCGGCAATAATTTTCACATTTATATTAAGCCGAATCGTCCGGTGGATCCCGAt                                                                                                                                                                               >  1:736994/1‑251 (MQ=255)
                                                                       ctactaATTTATTTCAAATGAGGCGATAATACCCG‑AAATTTTT‑GCGTATAAAAAGTAGCGTTATGAGTATTCAAATTAACCCGAATCGCCAAATTGT‑AATGGATACGGAAA‑CCACC‑GGTATGAACCAGTTAGGCGCCCATTATGAAGGTCA‑TTGCATCATT‑GAAAT‑CGGTGCGGTGGAAATGATTAACCGCCGTTATACCGGCAATAATTTTCACATTTATATTAAGCCGAATCGTCCGGTGGATCCCGAt                                                                                                                                                                               >  1:682160/1‑251 (MQ=255)
                                                                         actaATTTATTTCAAATGAGGCGATAATACCCG‑AAATTTTT‑GCGTATAAAAAGTAGCGTTATGAGTATTCAAATTAACCCGAATCGCCAAATTGT‑ACTGGATACGGAAA‑CCACC‑GGTATGAACCAGTTAGGCGCCCATTATGAAGGTCA‑TTGCATCATT‑GAAAT‑CGGTGCGGTGGAAATGATTAACCGCCGTTATACCGGCAATAATTTTCACATTTATATTAAGCCGAATCGTCCGGTGGATCCCGATg                                                                                                                                                                              >  1:102850/1‑250 (MQ=255)
                                                                            aaTTTATTTCAAATGAGGCGATAATACCCG‑AAATTTTT‑GCGTATAAAAAGTAGCGTTATGAGTATTCAAATTAACCCGAATCGCCAAATTGT‑ACTGGATACGGAAA‑CCACC‑GGTATGAACCAGTTAGGCGCCCATTATGAAGGTCA‑TTGCATCATT‑GAAAT‑CGGTGCGGTGGAAATGATTAACCGCCGTTATACCGGCa                                                                                                                                                                                                                              >  1:22111/1‑199 (MQ=255)
                                                                              tttatttCAAAGGAGGCAAAAATCCCCA‑AATTTTTT‑GCTTAAAAAAAGTAGCTTTAGGATTATTAAAATAACCCCGAATCGCAAAATTGT‑ACTGGATACGAAAC‑CCCCC‑GGTATGAACCAGTTAGGCGCCCATTATGAAGGTCA‑TTGCATCATG‑AAAAT‑CGGTGGGGGGAAAATGATTAACCGCCGTTATACCGGCAATAATTTTCACATTTATATTAAGCCGAATCGTCCGGTGGATCCCGATGCTATTa                                                                                                                                                                        <  1:767050/251‑1 (MQ=255)
                                                                                 ttttaaaaTGAGGCAATAATCCCCA‑AATTTTTT‑GTTTAAAAAAAGTAGCTTTATGAGTATCAAAATTACCCCGAACCGCAAATTTGT‑CCTGGATACGAAAA‑CCACC‑GGTATGAACCATTTGGGCGCCCATTATGAGGGTAA‑TTGAATCATG‑AAAAT‑GGGTGCGGTGAAAATGATTAACCGCCGTTATACCGGCAATAATTTTCACATTTATATTAAGCCGAATCGTCCGGTGGATCCCGATGCTATTAAgg                                                                                                                                                                     <  1:438402/246‑1 (MQ=255)
                                                                                 ttttaaaaTGAGGAAATAATCCCCA‑AAATTTTT‑GCTTAAAAAAAGTAGCGTTATGATTATTAAATTTACCCCGAATCCCCAAATTGT‑ATTGGATACGAAAC‑CCCCC‑GTTATAACCCAGTTAGCCCCCAATTATG‑AGGTTATTTGAATCATT‑AAAAT‑GGGTGCGGTGAAAATGATTAACCGCCGTTATACCGGCAATAATTTTCACATTTATATTAAGCCGAATCGTCCGGTGGATCCCGATGCTATTAAgg                                                                                                                                                                     <  1:371724/246‑1 (MQ=255)
                                                                                 ttttCAAATGAGGCGATAAT‑CCCC‑AAATTTTTGGCGTATAAAAAGTGGCGTTATGAGTATTAAAATTACCCCGAACCGCAAAATTGT‑ACTGAATAC‑GAAA‑CCCCCGGGTATGACCCATTTAGGCCCCAATTAGGAAGTTCA‑TTGCATCATT‑AAAAT‑CGGTGCGGTGAAAATGATTAACCGCCGTTATACCGGCAATAATTTTCACATTTATATTAAGCCGAATCGTCCGGTGGATCCCGATGCTATTAAgg                                                                                                                                                                     <  1:493609/250‑1 (MQ=255)
                                                                                 attaaaaaggAGGCGATAATCCCCG‑AAATTTTT‑GCGTAAAAAAAGTAGCGTTATGAGTATTAAAATTACCCCGAATCGCAAAATTGT‑ACTGGATACGGAAC‑CCACC‑GGTATGAACCAGTTGGGCGCCCATTATGAAGGTCA‑TTGCATCATT‑AAAAT‑CGGTGCGGTGGAAATGATTAACCGCCGTTATACCGGCAATAATTTTCACATTTATATTAAGCCGAATCGTCCGGTGGATCCCGATGCTATTAAgg                                                                                                                                                                     <  1:823527/242‑1 (MQ=255)
                                                                                 attaaaaaTGAGGCGATAATACCCG‑AATTTTTT‑GCATAAAAAAAGTAGCGTTATGAGTATACAAATTACCCCAAACCGCCAATTGGT‑ACGGGAGA‑GGAAACCCGCC‑GGTATGAACCAGTGAGCCGCCCATTATGAAGGTAA‑TTGCATAATT‑GAAAT‑CGGTGCGGCGAAAAGGATTAACCGCCGTTATACCGGCAATAATTTTCACATTTATATTAAGCCGAATCGTCCGGTGGATCCCGATGCTATTAAgg                                                                                                                                                                     <  1:404466/246‑1 (MQ=255)
                                                                                  ttacAAAGGAGGCGATAATACCCG‑AAATTTTT‑GCGTATAAAAAGTAGCGTTATGAGTATTAAAATTACCCCGAATCGCAAATTTGT‑ACTGGATAGGAAAA‑CCACC‑GGTATGACCCAGTTGGGCGCCCTTTAGGAAGGTCT‑TTCCATCATT‑AAAAT‑CGGTGCGGTGAAAATTATTAACCGCCGTTATACCGGCAATAATTTTCACATTTATATTAAGCCGAATCGTCCGGTGGATCCCGATGCTATTAAGGt                                                                                                                                                                    <  1:697298/248‑1 (MQ=255)
                                                                                    tCAAAGGAGGCAATAATCCCCA‑AATTTTTT‑GCGTAAAAAAAGTAGTTTTATGAGTATAAAAATTACCCCGAATCGCAAATTTGT‑ACTGGATACGAAAA‑CCACC‑GTTATGAACCAGTTAGGCGCCCATTATGAAGGTCA‑TTGCATCATT‑AAAAT‑CGGTGCGGTGAAAATGATTAACCGCCGTTATACCGGCAATAATTTTCACATTTATATTAAGCCGAATCGTCCGGTGGATCCCGATGCTATTAAGGTac                                                                                                                                                                  <  1:225922/251‑1 (MQ=255)
                                                                                          gAGGCGATAATACCCG‑AAATTTTT‑GCGTAAAAAAAGTAGCGTTATGAGTATTAAAATTACCCCGAATCGCAAAATTGT‑ACTGGATACGGAAA‑CCACC‑GGTATGAACCAGTAAGGCCCCCATTATGAAGGTCA‑TTGCATCATT‑AAAAT‑CGGGGGGGTGAAAATGATTAACCGCCGTTATACCGGCAATAATTTTCACATTTATATTAAGCCGAATCGTCCGGTGGATCCCGATGCTATTAAGGTACACGGt                                                                                                                                                             <  1:28137/250‑1 (MQ=255)
                                                                                            ggcaataatcCCCA‑AAATTTTT‑GCGAATAAAAAGTAGCGTTAGGAGTATCCAAATTACCCCGAATCGCAAATTTGT‑ACTGGATCCGAAAA‑CCCCC‑GGTATGAACCAGTTAGGCGCCCATTATGAAGTTCA‑TTCCATCATT‑GAAAT‑CGGTGCGGTGAAAATGATTAACCGCCGTTATACCGGCAATAATTTTCACATTTatat                                                                                                                                                                                                           <  1:732597/198‑1 (MQ=255)
                                                                                            ggCGATAATACCCG‑AAATTTTT‑GCGTATAAAAAGTAGCGTTATGAGTATTCAAATTAACCCGAATCGCCAAATTGT‑ACTGGATACGGAAA‑CCACC‑GGTATGAACCAGTTAGGCGCCCATTATGAAGGTCA‑TTGCATCATT‑GAAAT‑CGGTGCGGTGGAAATGATTAACCGCCGTTATACCGGCAATAATTTTCACATTTatat                                                                                                                                                                                                           >  1:414963/1‑202 (MQ=255)
                                                                                              aaataataCCCG‑AAATTTTT‑GCGTAAAAAAAGTAGCGTTAGGAGTATTAAAATTACCCCGAATCGCAAAATTGT‑ACTGGATACGGAAC‑CCACC‑GGTATGAACCAGTTAGGCGCCCTTTATGAAGGTCA‑TTGCATCATT‑GAAAT‑CGGTGGGGTGAAAATGATTAACCGCCGTTATACCGGCAATAATTTTCACATTTATATTAAGCCGAATCGTCCGGTGGATCCCGATGCTATTAAGGTACACGGTATCAc                                                                                                                                                        <  1:591861/249‑1 (MQ=255)
                                                                                                ataataCCCG‑AAATTTTT‑GCGTATAAAAAGTAGCGTTATGAGTATTCAAATTAACCCGAATCGCCAAATTGT‑ACTGGATACGGAAA‑CCACC‑GGTATGAACCAGTTAGGCGCCCATTATGAAGGTCA‑TTGCATCATT‑GAAAT‑CGGTGCGGTGGAAATGATTAACCGCCGTTATACCGGCAATAATTTTCACATTTATATTAAGCCGAATCGTCCGGTGGATCCCGATGCTATTAAGGTACACGGTATCACcg                                                                                                                                                      >  1:823296/1‑251 (MQ=255)
                                                                                                                        ataAAAAGTAGCGTTATGAGTATTCAAATTAACCCGAATCGCCAAATTGT‑ACTGGATACGGAAA‑CCACC‑GGTATGAACCAGTTAGGCGCCCATTATGAAGGTCA‑TTGCATCATT‑GAAAT‑CGGTGCGGTGGAAATGATTAACCGCCGTTATACCGGCAATAATTTTCACATTTATATTAAGCCGAATCGTCCGGTGGATCCCGATGCTATTAAGGTACACGGTATCACCGACGAAATGCTGGCGGACAAAc                                                                                                                                 >  1:450086/1‑250 (MQ=255)
                                                                                                                             aaggagtgTTATGAGTATAAAAATTAACCCGAACCGCAAAATTTT‑AGTGGAAACGAAAA‑CCACG‑GGTATGAACCAGTTAGGCGCCAATTATGAAGGTCA‑TTGCATCATT‑AAAAT‑GGGTGCGGTGAAAATGATTAACCGCCGTTATACCGGCAATAATTTTCACATTTATATTAAGCCGAATCGTCCGGTGGATCCCGATGCTATTAAGGTACACGGTATCACCGACGAAATGCTGGCGGACAAACCGGAtt                                                                                                                           <  1:297999/244‑1 (MQ=255)
                                                                                                                             aaGTAGCGTTATGAGTATTCAAATTACCCCGAATCCCCAAATTGT‑ACTGGATACGGAAC‑CCACC‑GGTATGAACCAGTTAGGCCCCCATTATGAAGTTCA‑TTGCATCATT‑AAAAT‑CGGTGCGGTGGAAATAATTAACCGCCGTTATACCGGCAATAATTTTCACATTTATATTAAGCCGAATCGTCCGGTGGATCCCGATGCTATTAAGGTACACGGTAt                                                                                                                                                           <  1:644462/219‑1 (MQ=255)
                                                                                                                             aaGTAGCGTTATGAGTATTCAAATTAACCCGAATCGCCAAATTGT‑ACTGGATACGGAAA‑CCACC‑GGTATGAACCAGTTAGGCGCCCATTATGAAGGTCA‑TTGCATCATT‑GAAAT‑CGGTGCGGTGGAAATGATTAACCGCCGTTATACCGGCAATAATTTTCACATTTATATTAAGCCGAATCGTCCGGTGGATCCCGATGCTATTAAGGTACACGGCATCACCGACGAAATGCTGGCGGACAAACCGGAtt                                                                                                                           >  1:638109/1‑251 (MQ=255)
                                                                                                                                  gCGTTATGAGTATTCAAATTAACCCGAATCGCCAAATTGT‑ACTGGATACGGAAA‑CCACC‑GGTATGAACCAGTTAGGCGCCCATTATGAAGGTCA‑TTGCATCATT‑GAAAT‑CGGTGCGGTGGAAATGATTAACCGCCGTTATACCGGCAATAATTTTCACATTTATATTAAGCCGAATCGTCCGGTGGATCCCGATGCTATTAAGGTACACGGTATCACCGACGAAATGCTGGCGGACAAACCGGATTtcagt                                                                                                                      >  1:218616/1‑251 (MQ=255)
                                                                                                                                   cGTTATGAGTATTCAAATTAACCCGAATCGCCAAATTGT‑ACTGGATACGGAAA‑CCACC‑GGTATGAACCAGTTAGGCGCCCATTATGAAGGTCA‑TTGCATCATT‑GAAAT‑CGGTGCGGTGGAAATGATTAACCGCCGTTATAc                                                                                                                                                                                                                                   >  1:169490/1‑141 (MQ=255)
                                                                                                                                   cGTTATGAGTATTAAAATAACCCCGAACCGCAAAATTGT‑ATGGGATA‑GGAAA‑CCCCCGGGTATGAACCAGTTAGGCGCCATTTATGAAGTTCA‑TTGCATCATT‑AAAAT‑CGGTGGGGTGAAAATGATAAACCGCCGTTATACCGGCAATAATTTTCACATTTATATTAAGCCGAATCGTCCGGTGGATCCCGATGCTATTAAGGTACACGGTATCACCGACGAAATGCTGGCGGACAAACCGGATTtcag                                                                                                                       <  1:316815/249‑1 (MQ=255)
                                                                                                                                    gTTATGAGTATAAAAATAACCCCAAACCGCAAATTTGT‑AGGGGATAGGGAAC‑CCACG‑GGTATGAACAAGTTGGGCGCCCATTAGGAGGTTCT‑TTGAATAATT‑GAAAT‑GGGGGGGGTGAAAATGATAAACCGCCGTTATACCGGCAATAATTTTCACATTTATATTAAGCCGAATCGTCCGGTGGATCCCGATGCTATTAAGGTACACGGTATCACCGACGAAATGCTGGCGGACAAACCGGATTtcagtc                                                                                                                     <  1:328728/250‑1 (MQ=255)
                                                                                                                                         gAGTATTCAAATTAACCCGAATCGCCAAATTGT‑ACTGGATACGGAAA‑CCACC‑GGTATGAACCAGTTAGGCGCCCATTATGAAGGTCA‑TTGCATCATT‑GAAAT‑CGGTGCGGTGGAAATGATTAACCGCCGTTATACCGGCAATAATTTTCACATTTATATTAAGCCGAATCGTCCGGTGGATCCCGATGCTATTAAGGTACACGGTATCACCGACGaa                                                                                                                                                 >  1:35046/1‑217 (MQ=255)
                                                                                                                                           gTATTCAAATTAACCCGAATCGCCAAATTGT‑ACTGGATACGGAAA‑CCACC‑GGTATGAACCAGTTAGGCGCCCATTATGAAGGTCA‑TTGCATCATT‑GAAAT‑CGGTGCGGTGGAAATGATTAACCGCCGTTATACCGGCAATAATTTTCACATTTatat                                                                                                                                                                                                           >  1:740131/1‑157 (MQ=255)
                                                                                                                                                aaaaTTAACCAGAATCGCAAAATTGT‑ACGGAATACGAAAA‑CCCCG‑GGTAGGACCCAGTTAGGCCCCAATTATAAAGGTCA‑TTGAATAATTAAAAAG‑GGGGGGGGTGAAAATGATTAACCGCCGTTATACCGGCAATAATTTTCACATTTATATTAAGCCGAATCGTCCGGTGGATCCCGATGCTATTAAGGTACACGGTATCACCGACGAAATGCTGGCGGACAAACCGGATTTCAGTCAGGCCGCCAATg                                                                                                         <  1:820265/250‑1 (MQ=255)
                                                                                                                                                      aaCCCGAATCGCCAAATTGT‑ACTGGATACGGAAA‑CCACC‑GGTATGAACCAGTTAGGCGCCCATTATGAAGGTCA‑TTGCATCATT‑GAAAT‑CGGTGCGGTGGAAATGATTAACCGCCGTTATACCGGCAATAATTTTCACATTTATATTAAGCCGAATCGTCCGGTGGATCCCGATGCTATTAAGGTACACGGTATCACCGACGAAATGCTGGCGGACAAACCGGATTTCAGTCAGGCCGCCAATGAATTCAt                                                                                                  >  1:735954/1‑251 (MQ=255)
                                                                                                                                                       aCCCGAATCGCCAAATTGT‑ACTGGATACGGAAA‑CCACC‑GGTATGAACCAGTTAGGCGCCCATTATGAAGGTCA‑TTGCATCATT‑GAAAT‑CGGTGCGGTGGAAATGATTAACCGCCGTTATACCGGCAATAATTTTCACATTTATATTAAGCCGAATCGTCCGGTGGATCCCGATGCTATTAAGGTACACGGTATCACCGACGAAATGCTGGCGGACAAACCGGATTTCAGTCAGGCCGCCAATGAATTCAt                                                                                                  >  1:164111/1‑250 (MQ=255)
                                                                                                                                                                   aaaTTGT‑ACTGGATACGGAAA‑CCACC‑GGTATGAACCAGTTAGGCGCCCATTATGAAGGTCA‑TTGCATCATT‑GAAAT‑CGGTGCGGTGGAAATGATTAACCGCCGTTATACCGGCAATAATTTTCACATTTATATTAAGCCGAATCGTCCGGTGGATCCCGATGCTATTAAGGTACACGGTATCACCGACGAAATGCTGGCGGACAAACCGGATTTCAGTCAGGCCGCCAATGAATTCATCGAATATATCaaa                                                                                     >  1:515847/1‑251 (MQ=255)
                                                                                                                                                                   aaaTTGT‑ACTGGATACGAAAC‑CCCCC‑GGTATAACCCAGTTGGGCGCCCATTATGAAGGTCT‑TTGCATCATT‑AAAAT‑GGGTGCGGTGAAAATGATTAACCGCCGTTATACCGGCAATAATTTTCACATTTATATTAAGCCGAATCGTCCGGTGGATCCCGATGCTATTAAGGTACACGGTATCACCGACGAAATGCTGGCGGACAAACCGGATTTCAGTCAGGCCGCCAATGAATTCATCGAATATATCaaa                                                                                     <  1:230219/251‑1 (MQ=255)
                                                                                                                                                                     tttGT‑AGTGGATACGAAAA‑CCCCC‑GGTAAGACCCGGTTAGGCCCCTATTATGAAGGTCA‑TCGCATCATT‑AAAAG‑GGGTGGGGTGAAAATGATAAACCGCCGTTATACCGGCAATAATTTTCACATTTATATTAAGCCGAATCGTCCGGTGGATCCCGATGCTATTAAGGTACACGGTATCACCGACGAAATGCTGGCGGACAAACCGGATTTCAGTCAGGCCGCCAATGAATTCATCGAATATATCAAAgg                                                                                   <  1:701577/250‑1 (MQ=255)
                                                                                                                                                                     tttGT‑ACGGGATACGAAAC‑CCCCC‑GGTATGAACCAGTTGGGCGCCCATTATGAAGTTCA‑TTCCATCATT‑AAAAG‑CGGTGCGGTGAAAATAATTAACCGCCGTTATACCGGCAATAATTTTCACATTTATATTAAGCCGAATCGTCCGGTGGATCCCGATGCTATTAAGGTACACGGTATCACCGACGAAATGCTGGCGGACAAACCGGATTTCAGTCAGGCCGCCAATGAATTCATCGAATATATCAAAgg                                                                                   <  1:51614/250‑1 (MQ=255)
                                                                                                                                                                     aTTGT‑ACTGGATACGGAAA‑CCACC‑GGTATGAACCAGTTAGGCGCCCATTATGAAGGTCA‑TTGCATCATT‑GAAAT‑CGGTGCGGTGGAAATGATTAACCGCCGTTATACCGGCAATAATTTTCACATTTATATTAAGCCGAATCGTCCGGTGGATCCCGATGCTATTAAGGTACACGGTATCACCGACGAAATGCTGGCGGACAAACCGGATTTCAGTCAGGCCGCCAATGAATTCATCGAATATATCAAAgg                                                                                   >  1:498987/1‑251 (MQ=255)
                                                                                                                                                                     aTTGT‑ACTGGATACGGAAA‑CCACC‑GGTATGAACCAGTTAGGCCCCCATTATGAAGTTCA‑TTGCATCATT‑GAAAT‑CGGTGCGGTGAAAATGATTAACCGCCGTTATACCGGCAATAATTTTCACATTTATATTAAGCCGAATCGTCCGGTGGATCCCGATGCTATTAAGGTACACGGTATCACCGACGAAATGCTGGCGGACAAACCGGATTTCAGTCAGGCCGCCAATGAATTCATCGAATATATCAAAgg                                                                                   <  1:263442/251‑1 (MQ=255)
                                                                                                                                                                        gT‑ACTGGATACGGAAA‑CCACC‑GGTATGAACCAGTTAGGCGCCCATTATGAAGGTCA‑TTGCATCATT‑GAAAT‑CGGTGCGGTGGAAATGATTAACCGCCGTTATACCGGCAATAATTTTCACATTTATATTAAGCCGAATCGTCCGGTGGATCCCGATGCTATTAAGGTACACGGTATCACCGACGAAATGCTGGCGGACAAACCGGATTTCAGTCAGGCCGCCAATGAATTCATCGAATATATCAAAGgcg                                                                                 >  1:238579/1‑250 (MQ=255)
                                                                                                                                                                              ggATACGGAAA‑CCACC‑GGTATGAACCAGTTAGGCGCCCATTATGAAGGTCA‑TTGCATCATT‑GAAAT‑CGGTGCGGTGGAAATGATTAACCGCCGTTATACCGGCAATAATTTTCACATTTATATTAAGCCGAATCGTCCGGTGGATCCCGATGCTATTAAGGTACACGGTATCACCGACGAAATGCTGGCGGACAAACCGGATTTCAGTCAGGCCGCCAATGAATTCATCGAATATATCAAAGGCGCCGAGt                                                                           >  1:335892/1‑251 (MQ=255)
                                                                                                                                                                                 tACGGAAA‑CCACC‑GGTATGAACCAGTTAGGCGCCCATTATGAAGGTCA‑TTGCATCATT‑GAAAT‑CGGTGCGGTGGAAATGATTAACCGCCGTTATACCGGCAATAATTTTCACATTTATATTAAGCCGAATCGTCCGGTGGATCCCGATGCTATTAAGGTACACGGTATCACCGACGAAATGCTGGCGGACAAACCGGATTTCAGTCAGGCCGCCAATGAATTCATCGAATATATCAAAGGCGCCGAGTTg                                                                         >  1:660171/1‑250 (MQ=255)
                                                                                                                                                                                       aa‑CCACC‑GGTATGAACCAGTTAGGCGCCCATTATGAAGGTCA‑TTGCATCATT‑GAAAT‑CGGTGCGGTgg                                                                                                                                                                                                                                                         <  1:208331/68‑1 (MQ=255)
                                                                                                                                                                                       aa‑CCACC‑GGTATGAACCAGTTAGGCCCCCTTTAGGAAGGTCA‑TTGCATAATT‑AAAAT‑CGGTGCGGTGAAAATGATTAACCGCCGTTATACCGGCAATAATTTTCACATTTATATTAAGCCGAATCGTCCGGTGGATCCCGATGCTATTAAGGTACACGGt                                                                                                                                                             <  1:600819/160‑1 (MQ=255)
                                                                                                                                                                                           ccacgggaaTGAACCATTTGGGCGCCCATTAGGAAGGTCA‑TTGCATCATT‑AAAAT‑CGGTGCGGTGAAAATTATTAACCGCCGTTATACCGGCAATAATTTTCACATTTATATTAAGCCGAATCGTCCGGTGGATCCCGATGCTATTAAGGTACACGGTATCACCGACGAAATGCTGGCGGACAAACCGGATTTCAGTCAGGCCGCCAATGAATTCATCGAATATATCAAAGGCGCCGAGTTGCTTATTcac                                                                <  1:396564/243‑1 (MQ=255)
                                                                                                                                                                                             cccggtaggaccaagtgaggccccaaTTATGAAGGTCA‑TTGCATAATT‑GAAAT‑CGGTGGGGGGAAAATGATTAACCGCCGTTATACCGGCAATAATTTTCACATTTATATTAAGCCGAATCGTCCGGTGGATCCCGATGCTATTAAGGTACACGGTATCACCGACGAAATGCTGGCGGACAAACCGGATTTCAGTCAGGCCGCCAATGAATTCATCGAATATATCAAAGGCGCCGAGTTGCTTATTCACaa                                                              <  1:399197/226‑1 (MQ=255)
                                                                                                                                                                                                            ttAGGCCCCCATTATAAAGGTAA‑TTGCATAATG‑AAAAT‑CGGTGGGGTGGAAATGTTTACCCGCCGTTATACCGGCAATAATTTTCACATTTATATTAAGCCGAATCGTCCGGTGGATCCCGATGCTATTAAGGTACACGGTATCACCGACGAAATGCTGGCGGACAAACCGGATTTCAGTCAGGCCGCCAATGAATTCATCGAATATATCAAAGGCGCCGAGTTGCTTATTCACAACGCGCCCTTCGACg                                                <  1:189294/250‑1 (MQ=255)
                                                                                                                                                                                                                       ttagaaagttCA‑TTGCTTCATT‑‑AAATGCGGTGCGGGGGAAATAATAAACCGCCGTTATACCGGCAATAATTTTCACATTTATATTAAGCCGAATCGTCCGGTGGATCCCGATGCTATTAAGGTACACGGTATCACCGACGAAATGCTGGCGGACAAACCGGATTTCAGTCAGGCCGCCAATGAATTCATCGAATATATCAAAGGCGCCGAGTTGCTTATTCACAACGCGCCCTTCGACGTGGGCTTCATgg                                    <  1:409340/242‑1 (MQ=255)
                                                                                                                                                                                                                       ttATGAAGGTCA‑TTGCATCATT‑GAAAT‑CGGTGCGGTGGAAATGATTAACCGCCGTTATACCGGCAATAATTTTCACATTTATATTAAGCCGAATCGTCCGGTGGATCCCGATGCTATTAAgg                                                                                                                                                                     >  1:57372/1‑122 (MQ=255)
                                                                                                                                                                                                                       ttATGAAGGTCA‑TTGCATCATT‑GAAAT‑CGGTGCGGTGGAAATGATTAACCGCCGTTATACCGGCAATAATTTTCACATTTATATTAAGCCGAATCGTCCGGTGGATCCCGATGCTATTAAGGTACACGGTATCACCGACGAAATGCTGGCGGACAAACCGGATTTCAGTCAGGCCGCCAATGAATTCATCGAATATATc                                                                                        >  1:352346/1‑199 (MQ=255)
                                                                                                                                                                                                                       ttATGAAGGTCA‑TTGCATCATT‑GAAAT‑CGGTGCGGTGGAAATGATTAACCGCCGTTATACCGGCAATAATTTTCACATTTATATTAAGCCGAATCGTCCGGTGGATCCCGATGCTATTAAGGTACACGGTATCACCGACGAAATGCTGGCGGACAAACCGGATTTCAGTCAGGCCGCCAATGAATTCATCGAATATATCAAAGGCGCCGAGTTGCTTATTCACAACGCGCCCTTCGACg                                                >  1:27985/1‑239 (MQ=255)
                                                                                                                                                                                                                       ttATGAAGGTCA‑TTGCATCATT‑GAAAT‑CGGTGCGGTGGAAATGATTAACCGCCGTTATACCGGCAATAATTTTCACATTTATATTAAGCCGAATCGTCCGGTGGATCCCGATGCTATTAAGGTACACGGTATCACCGACGAAATGCTGGCGGACAAACCGGATTTCAGTCAGGCCGCCAATGAATTCATCGAATATATCAAAGGCGCCGAGTTGCTTATTCACAACGCGCCCTTCGACGTGGGCTTCATgg                                    >  1:439931/1‑251 (MQ=255)
                                                                                                                                                                                                                       ttATGAAGGTCA‑TTGCATCATT‑GAAAT‑CGGTGCGGTGGAAATGATTAACCGCCGTTATACCGGCAATAATTTTCACATCTATATTAAGCCGAATCGTCCGGTGGATCCCGATGCTATTAAGGTACACGGTATCACCGACGAAATGCTGGCGGACAAACCGGATTTCAGTCAGGCCGCCAATGAATTCATCGAATATATCAAAGGCGCCGAGTTGCTTATTCACAACGCGCCCTTCGACGTGGGCTTCATgg                                    >  1:126642/1‑251 (MQ=255)
                                                                                                                                                                                                                        tATGAAGTTAA‑TTGCATCATT‑AAAGG‑CGGTGGGGTGAAAATGATTAACCGCCGTTATACCGGCAATAATTTTCACATTTATATTAAGCCGAATCGTCCGGTGGATCCCGATGCTATTAAGGTACACGGTATCACCGACGAAATGCTGGCGGACAAACCGGATTTCAGTCAGGCCGCCAATGAATTCATCGAATATATCAAAGGCGCCGAGTTGCTTATTCACAACGCGCCCTTCGACGTGGGCTTCATGGa                                   <  1:80028/251‑1 (MQ=255)
                                                                                                                                                                                                                        tATGAAGGTAA‑TTCAATAATT‑AAAAT‑GGGTGGGGTGAAAATGATTAACCGCCGTTATACCGGCAATAATTTTCACATTTATATTAAGCCGAATCGTCCGGTGGATCCCGATGCTATTAAGGTACACGGTATCACCGACGAAATGCTGGCGGACAAACCGGATTTCAGTCAGGCCGCCAATGAATTCATCGAATATATCAAAGGCGCCGAGTTGCTTATTCACAACGCGCCCTTCGACGTGGGCTTCATGGa                                   <  1:661053/251‑1 (MQ=255)
                                                                                                                                                                                                                         aTGAAGGTCA‑TTGCATCATT‑GAAAT‑CGGTGCGGTGGAAATGATTAACCGCCGTTATACCGGCAATAATTTTCACATTTATATTAAGCCGAATCGTCCGGTGGATCCCGATGCTATTAAGGTACACGGTATCACCGACGAAATGCTGGCGGACAAACCGGATTTCAGTCAGGCCGCCAATGAATTCATCGAATATATCAAAGGCGCCGAGTTGCTTATTCACAACGCGCCCTTCGACGTGGGCTTCATGGAt                                  >  1:556396/1‑251 (MQ=255)
                                                                                                                                                                                                                              ggTCA‑TTGCATCATT‑GAAAT‑CGGTGCGGTGGAAATGATTAACCGCCGTTATACCGGCAATAATTTTCACATTTATATTAAGCCGAATCGTCCGGTGGATCCCGATGCTATTAAGGTACACGGTATCACCGACGAAATGCTGGCGGACAAACCGGATTTCAGTCAGGCCGCCAATGAATTCATCGAATATATCAAAGGCGCCGAGTTGCTTATTCACAACGCGCCCTTCGACGTGGGCTTCATGGATTATGa                             >  1:763507/1‑251 (MQ=255)
                                                                                                                                                                                                                                tCA‑TTACATCATT‑AAAAT‑GGGTGCGGTGAAATTGATTAACCGCCGTTATACCGGCAATAATTTTCACATTTATATTAAGCCGAATCGTCCGGTGGATCCCGATGCTATTAAGGTACACGGTATCACCGACGAAATGCTGGCGGACAAACCGGATTTCAGTCAGGCCGCCAATGAATTCATCGAATATATCAAAGGCGCCGAGTTGCTTATTCACAACGCGCCCTTCGACGTGGGCTTCATGGATTATGaa                            <  1:45727/250‑1 (MQ=255)
                                                                                                                                                                                                                                  tatttcaatcatgaaaaT‑GGGTGCGTTGAAAATGATTAACCGCCGTTATACCGGCAATAATTTTCACATTTATATTAAGCCGAATCGTCCGGTGGATCCCGATGCTATTAAGGTACACGGTATCACCGACGAAATGCTGGCGGACAAACCGGATTTCAGTCAGGCCGCCAATGAATTCATCGAATATATCAAAGGCGCCGAGTTGCTTATTCACAACGCGCCCTTCGACGTGGGCTTCATGGATTATGaa                            <  1:214051/236‑1 (MQ=255)
                                                                                                                                                                                                                                   gctgggagatgtataaaagagaagggtgAAAATGATTAACCGCCGTTATACCGGCAATAATTTTCACATTTATATTAAGCCGAATCGTCCGGTGGATCCCGATGCTATTAAGGTACACGGTATCACCGACGAAATGCTGGCGGACAAACCGGATTTCAGTCAGGCCGCCAATGAATTCATCGAATATATCAAAGGCGCCGAGTTGCTTATTCACAACGCGCCCTTCGACGTGGGCTTCATGGATTATGaa                            <  1:164818/226‑1 (MQ=255)
                                                                                                                                                                                                                                           atT‑GAAAT‑CGGTGCGGTGGAAATGATTAACCGCCGTTATACCGGCAATAATTTTCACATTTATATTAAGCCGAATCGTCCGGTGGATCCCGATGCTATTAAGGTACACGGTATCACCGACGAAATGCTGGCGGACAAACCGGATTTCAGTCAGGCCGCCAATGAATTCATCGAATATATCAAAGGCGCCGAGTTGCTTATTCACAACGCGCCCTTCGACGTGGGCTTCATGGATTATGAATTCGCCAAg                   >  1:605784/1‑249 (MQ=255)
                                                                                                                                                                                                                                           atT‑GAAAT‑CGGTGCGGTGGAAATGATTAACCGCCGTTATACCGGCAATAATTTTCACATTTATATTAAGCCGAATCGTCCGGTGGATCCCGATGCTATTAAGGTACACGGTATCACCGACGAAATGCTGGCGGACAAACCGGATTTCAGTCAGGCCGCCAATGAATTCATCGAATATATCAAAGGCGCCGAGTTGCTTATTCACAACGCGCCCTTCGACGTGGGCTTCATGGATTATGAATTCGCCAAg                   >  1:529886/1‑249 (MQ=255)
                                                                                                                                                                                                                                                aaaT‑CGGTGGGGTGAAAATGATTAACCGCCGTTATACCGGCAATAATTTTCACATTTATATTAAGCCGAATCGTCCGGTGGATCCCGATGCTATTAAGGTACACGGTATCACCGACGAAATGCTGGCGGACAAACCGGATTTCAGTCAGGCCGCCAATGAATTCATCGAATATATCAAAGGCGCCGAGTTGCTTATTCACAACGCGCCCTTCGACGTGGGCTTCATGGATTATGAATTCGCCAAGCTGAAc             <  1:143108/251‑1 (MQ=255)
                                                                                                                                                                                                                                                            gtgGAAATGATTAACCGCCGTTATACCGGCAATAATTTTCACATTTATATTAAGCCGAATCGTCCGGTGGATCCCGATGCTATTAAGGTACACGGTATCACCGACGAAATGCTGGCGGACAAACCGGATTTCAGTCAGGCCGCCAATGAATTCATCGAATATATCAAAGGCGCCGAGTTGCTTATTCACAACGCGCCCTTCGACGTGGGCTTCATGGATTATGAATTCGCCAAGCTGAACCTGCCGATTaa  >  1:811521/1‑251 (MQ=255)
                                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                       
GATACCGCCCGCACCGGGGTTGCCGAGGCAGGATCCGTCGGTAAAAATTTCGATTTGTTTGCGCATATTGACTACTAATTTATTTCAAATGAGGCGATAATACCCG‑AAATTTTT‑GCGTATAAAAAGTAGCGTTATGAGTATTCAAATTAACCCGAATCGCCAAATTGT‑ACTGGATACGGAAA‑CCACC‑GGTATGAACCAGTTAGGCGCCCATTATGAAGGTCA‑TTGCATCATT‑GAAAT‑CGGTGCGGTGGAAATGATTAACCGCCGTTATACCGGCAATAATTTTCACATTTATATTAAGCCGAATCGTCCGGTGGATCCCGATGCTATTAAGGTACACGGTATCACCGACGAAATGCTGGCGGACAAACCGGATTTCAGTCAGGCCGCCAATGAATTCATCGAATATATCAAAGGCGCCGAGTTGCTTATTCACAACGCGCCCTTCGACGTGGGCTTCATGGATTATGAATTCGCCAAGCTGAACCTGCCGATTAA  >  1/1999004‑1999498

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑