| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | 1 | 1,973,297 | 0 | C | T | 38.0% | 86.6 / 26.6 | 71 | intergenic (+123921/‑26695) | unknown/unknown | tRNA‑Leu‑CAA/tRNA‑Asp‑GTC |
| Reads supporting (aligned to +/- strand): ref base C (19/25); new base T (27/0); total (46/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.42e-07 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AAGACAAAATCCAAGGATTTTGAACGTTGGCTTTGCCAATGGTACGAAGTGCAAGCGCAGCGCAGTAAAAGTAGGTCGATGCCGAAACAAACCTATTTATATCGCTTCTTACCACTTTCCTGATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTC‑‑GGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGGAAGTAGTTTTCGCCGGCAGCGTAGGCG‑TTAGGTTCATTGTGGGCGTAGCGGTATTCGGCGC‑C‑GAAGCCGAGGTCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGACGTC‑GAAATCGGC‑GGA‑TTC‑GGTT‑G‑CC‑AATTTTTTGGCGGC‑TTTGAAAGCGAGATAAACGGCGTTGCTTTTCGGTGCGACGGCTAAATACACAATGGCTTGCGCAATAGCGCGTTCGCCTTCGGCAGCGCCGACG > 1/1973068‑1973535 | aaGACAAAATCCAAGGATTTTGAACGTTGGCTTTGCCAATGGTACGAAGTGCAAGCGCAGCGCAGTAAAAGTAGGTCGATGCCGAAACAAACCTATTTATATCGCTTCTTACCACTTTCCTGATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTC‑‑GGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGGTAGACGTTTTCGCCCGCacgggaggggttcgg > 1:146145/1‑237 (MQ=255) aaTCCAAGGATTTTGAACGTTGGCTTTGCCAATGGTACGAAGTGCAAGCGCAGCGCAGTAAAAGTAGGTCGATGCCGAAACAAACCTATTTATATCGCTTCTTACCACTTTCCTGATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTC‑‑GGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGGAAGTAGTTTTCGCCGGCAGCGTAGGCG‑TTAGGTTCATtg < 1:640252/251‑1 (MQ=255) gATTTTGAACGTTGGCTTTGCCAATGGTACGAAGTGCAAGCGCAGCGCAGTAAAAGTAGGTCGATGCCGAAACAAACCTATTTATATCGCTTCTTACCACTTTCCTGATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTC‑‑GGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGGGAGGAGGTTTTGCCGGCAGCGGAGGCG‑TTTGGttt > 1:795369/1‑238 (MQ=255) ggCTTTGCCAATGGTACGAAGTGCAAGCGCAGCGCAGTAAAAGTAGGTCGATGCCGAAACAAACCTATTTATATCGCTTCTTACCACTTTCCTGATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTC‑‑GGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGGGAGTAGGTTTTGCCCGCAGCGGAGGCG‑TTTGGTTTATTGTGGGCGGAGCGGTATTTGgcg > 1:501240/1‑251 (MQ=255) tGCCAATGGTACGAAGTGCAAGCGCAGCGCAGTAAAAGTAGGTCGATGCCGAAACAAACCTATTTATATCGCTTCTTACCACTTTCCTGATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTC‑‑GGGAAATAGACTTGGGTGTCTTTCAGTTGTGGCGGGGAGTAGTTTTTGCCGGCAGCGCAGGCG‑TCAGGTTCTTTGTGGGGGTAGCGGGATTCGGCGC‑C‑Gaa > 1:494607/1‑251 (MQ=255) gaTGGTACGAAGTGCAAGCGCAGCGCAGTAAAAGTAGGTCGATGCCGAAACAAACCTATTTATATCGCTTCTTACCACTTTCCTGATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTC‑‑GGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGGAAGTAGTTTTTGCCCGCCGCGTAGGCG‑GTAGGTTCATTGTGGGCGGAGCGGTATTCGGCGC‑C‑GCAGCCg > 1:97596/2‑251 (MQ=255) tGGTACGAAGTGCAAGCGCAGCGCAGTAAAAGTAGGTCGATGCCGAAACAAACCTATTTATATCGCTTCTTACCACTTTCCTGATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTC‑‑GGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGGAAGTAGTTTTTGCCGGCCGCGGAGGCg > 1:413684/1‑207 (MQ=255) ggTACGAAGTGCAAGCGCAGCGCAGTAAAAGTAGGTCGATGCCGAAACAAACCTATTTATATCGCTTCTTACCACTTTCCTGATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTC‑‑GGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGGAAATAGGTTTCGCCGGCAGCGGAGGCG‑GTAGGGTCCTTGTGGGGGTAGGGGTGTTTGGGGC‑C‑GGAGCCGAgg > 1:484087/1‑251 (MQ=255) aaGTGCAAGCGCAGCGCAGTAAAAGTAGGTCGATGCCGAAACAAACCTATTTATATCGCTTCTTACCACTTTCCTGATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTC‑‑GGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGGAAGTAGTTTTCGCCGGCAGCGGAGGCG‑GTAGGGTCATTGTGGGCGGAGCGGGATTCGGGGC‑C‑GGAGCCGGGGGCtttt > 1:292253/1‑250 (MQ=255) cAAGCGCAGCGCAGTAAAAGTAGGTCGATGCCGAAACAAACCTATTTATATCGCTTCTTACCACTTTCCTGATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTC‑‑GGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGGAAGTAGTTTTCGCCGGCAGCGTAGGCG‑TTAGGTTCATTGTGGGCGTAGCGGTATTCGGCGC‑C‑GAAGCCGAGGTCTTTCATGa < 1:645574/250‑1 (MQ=255) aaGTAGGTCGATGCCGAAACAAACCTATTTATATCGCTTCTTACCACTTTCCTGATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTC‑‑GGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGGAAGGAGTTTTTGCCGGCAGCGGAGGCG‑GTAGGGTCATTGTGGGGGGAGCGGGATTTGGCGC‑C‑GAAGCCGAGGGCTTTCAt > 1:745374/1‑231 (MQ=255) cGAAACAAACCTATTTATATCGCTTCTTACCACTTTCCTGATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTC‑‑GGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGGAAGTAGTTTTCGCCGGCAGCGTAGGCG‑TTAGGTTCATTGTGGGCGTAGCGGTATTCGGCGC‑C‑GAAGCCGAGGTCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAggg < 1:645933/251‑1 (MQ=255) aaacaaacCTATTTATATCGCTTCTTACCACTTTCCTGATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTC‑‑GGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGGAAGTAGTTTTCGCCGGCAGCGTAGGCG‑TTAGGTTCATTGTGGGCGTAGCGGTATTCGGCGC‑C‑GAAGCCGAGGTCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGa < 1:435462/250‑1 (MQ=255) aaacaaacCTATTTATATCGCTTCTTACCACTTTCCTGATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTC‑‑GGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGGAAGTAGTTTTCGCCGGCAGCGTAGGCG‑TTAGGTTCATTGTGGGCGTAGCGGTATTCGGCGC‑C‑GAAGCCGAGGTCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGa < 1:56742/250‑1 (MQ=255) aacCTATTTATATCGCTTCTTACCACTTTCCTGATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTC‑‑GGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGGAAGTAGGTTTTTCCCGCCGCGGAGGCG‑GTAGGTTTATTTTGGGCGTAGCGGGATTCGG‑GCGC‑GCAGCCGAGGGCTTTTTTGGAGCTGGTGGGGGGGGTGCGGGGGTGTTGAggggggtcgg > 1:229855/1‑244 (MQ=255) ccTATTTATATCGCTTCTTACCACTTTCCTGATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTC‑‑GGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGGGAGGAGTTTTTGCCGGCAGCGTAGGCG‑TTAGGTTCATTTTGGGGGGAGCGGTATTCGGGGC‑C‑GAAGCCGAGGGCTTTCATGAAGCTGGGCGGGGGGTTGCGGGGATGTTCAggggagtcggaa > 1:318442/1‑242 (MQ=255) ccTATTTATATCGCTTCTTACCACTTTCCTGATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTC‑‑GGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGGAAGGAGGTTTCGCCGGCAGCGTAGGCG‑GTTGGGTCATTGTGGGCGGAGCGGGATTCGGCGC‑C‑GAAGCCGAGGGCTTTCATGAAGCTGGTCGGGGCGTTGCgggg > 1:390795/1‑230 (MQ=255) aTTTATATCGCTTCTTACCACTTTCCTGATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTC‑‑GGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGGGAGTAGGTTTTGCCGGCAGCGGAGGGGTTTGGGTTC‑TTGTGGGGGGGGCGGGATTTTGCGC‑C‑GCCGCCGGGGGCTTTTATGAAGCTGGTCGGGGCCTTTCGgaggtggttcggggcgtcggcat > 1:479902/1‑229 (MQ=255) aTTTATATCGCTTCTTACCACTTTCCTGATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTC‑‑GGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGGAAGTAGTTTTTGCCGGCCGCGTAGGCG‑TTTGGTTTATTGTGGGGGGAGCGGGATTCGGCGC‑C‑GAAGCCGAGGGCTTTCATGAAGCTGGTCGGGGCGGTTCGGGGATGTTCAGGGGCGGC‑GGAATcg > 1:515450/1‑251 (MQ=255) cGCTTCTTACCACTTTCCTGATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTC‑‑GGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGGAAGTAGTTTTCGCCGGCAGCGTAGGCG‑TTAGGTTCATTGTGGGCGTAGCGGTATTCGGCGC‑C‑GAAGCCGAGGTCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGACGTC‑GAAATCGGC‑GGA‑TTc < 1:38871/251‑1 (MQ=255) tcttACCACTTTCCTGATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTC‑‑GGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGGGAGGAGGTTTTGCCGGCAGCGGAGGGG‑TTTGGGTTATTTTGGGGGGGGCGGGATTTGGCGC‑C‑GGAGCCGAGGGCTTTCATGAAGCTGGTCgggggggtgggggga > 1:525160/1‑207 (MQ=255) tcttACCACTTTCCTGATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTC‑‑GGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGGAAGTAGTTTTCCCCGGCCGCGCAGGCG‑GTAGGTTCTTTGTGGGCGTCGTGGGATTTGGCGC‑C‑GCAGGCGTGGTTTTTTTTGAAGCTGGTCGGGGCGGTGCGGGGGTGTTTAGGGACGCT‑GAAATCGGG‑GGA‑ttttggt > 1:316380/1‑246 (MQ=255) tcttACCACTTTCCTGATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTC‑‑GGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGGAAATAGTTTTTGCCGGCAACGATGGCG‑GTGGGGTTGTTGTGGGCGCAGCGGTATTTGGCGC‑C‑GCAGCCGAGGGGTTTTCTGGAACTCGTTGGGGCGTTGAGGAGATGTTCTGGGACATC‑GAAAATGGC‑GGA‑TTC‑CGtt > 1:687956/1‑251 (MQ=255) ttCCTGATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGGGAGTAGTTTTCGCCGGCCGCGGAGGCG‑TTTGGTTCATTGTGGGCGTAGCGGGATTCGGCGC‑C‑GCAGCCGAggg > 1:166520/1‑177 (MQ=255) ttCCTGATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTC‑‑GGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGGGAGGAGTTTTCGCCGGCAGGGGAGGCG‑GTCGGGTTATTGTGGGGGCAGCGGGATTCGGCGG‑C‑GAAGCCGCGGGCTTTTCTTAAGCTGGGCGGGGGGGTGCGGGGACGGTTAGGGGGGTC‑GGAATcggc‑gggagcgggtggccactttt > 1:139019/1‑233 (MQ=255) ttCCTGATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTC‑‑GGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGGGAGGAGTTTTCGCCGGCAGCGGAGGCG‑GTAGGGTCATTGTGGGGGGAGCGGTATTCGGGGC‑C‑GGAGCCGAGGGCTTTCCTGAAGGTGGTCGGGGGGGTGCGGGGGTGGTCAGGGGCGTC‑GGAATcggc‑ggggtcgggtggccattttt > 1:297890/1‑233 (MQ=255) ttCCTGATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTC‑‑GGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGGAAGTAGTTTTCGCCGGCCGCGGAGGCG‑TTAGGTTCATTGTGGGCGTAGCGGTATTCGGCGC‑C‑GAAGCCGAGGGCTTTCATGAAGCTGGTCGGGGCGGTGCGGGGATGTTCAGGGGCGGC‑GAAATCGGC‑GGG‑TTT‑GGGT‑G‑CC‑CAttttt > 1:189089/1‑251 (MQ=255) gATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTC‑‑GGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGGGAGTAAGTTTCCCCGGCAGCGGAGGGG‑GTTGGGTCATTGTGGGCGTAGCGGGATTCCGCGC‑C‑GAAGGCGAGGGGTTTCATGAAGCTGGGCGGGGGGGTGCGGGGGAGTTTCGGGGCGTC‑GGAAACGGCGGGA‑TTC‑GGTG‑G‑CC‑ATTTTTTTgcg > 1:745507/1‑249 (MQ=255) gATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTC‑‑GGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGGAAGGAGTTTTCGCCGGCAGCGGAGGCG‑TTAGGGTCATTGTGGGCGGAGCGGGATTCGGCGC‑C‑GAAGCCGAGGGCTTTCATGAAGCTGGTCGGGGCGGTGCGGGGAAGTTCAGGGACGTC‑GAAAACGGC‑GGG‑TTT‑GGGT‑G‑CC‑CATTTTTTtgcg > 1:327851/1‑251 (MQ=255) aaCCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTC‑‑GGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGGGAGGAGTTTTTGCCGGCCGCGGAGGCG‑GTAGGGTCATTGTGGGGGGGGCGGTATTTGGCGC‑C‑GGAGCCGGGGGCTTTCCTGAAGCTGGGCGGGGGGGTGCGGGGATGGTTAGGGGCGTC‑GCAATCGGG‑GGG‑TTT‑GGTTGG‑CC‑AATTTTTTgtgggttt > 1:507908/1‑244 (MQ=255) cccccGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTC‑‑GGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGGAAGTAGTTTTTGCCGGCAGCGGAGGCG‑TTAGGGTTATTGTGGGGGTAGCGGGATTCGGCGC‑C‑GGAGCCGAGGGCTTTCATGAAGCTGGTCGGGGCGGTGCGGGGAAGTTCAGGGGCGTC‑GAAATAGGG‑GGG‑GTC‑GGGT‑G‑GC‑AAATTTTTTGCGGC‑TTTgt > 1:119898/1‑250 (MQ=255) cccGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTC‑‑GGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGGAAGTAGTTTTTGCCGGCAGCGTAGGCG‑TTAGGTTCATTGTGGGGGGAGCGGGATTCGGCGC‑C‑GAAGCCGAGGGCTTTCATGAAGCTGGTCGGGGCGGTGCGGGGATGTTCAGGGACGTC‑GGAATCGGCGGGG‑TTG‑GGTG‑G‑CA‑AATTTTTTGGGGGT‑TTTGaa > 1:239876/1‑251 (MQ=255) ccGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTC‑‑GGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGGAAGTAGTTTTCGCCGGCAGCGTAGGCG‑TTAGGTTCATTGTGGGCGTAGCGGTATTCGGCGC‑C‑GAAGCCGAGGTCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGACGTC‑GAAATCGGC‑GGA‑TTC‑GGTT‑G‑CC‑AATTTTTTGGCGGC‑TTTGAAAg < 1:604972/251‑1 (MQ=255) ttCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTC‑‑GGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGGGAGGAGGTTTTGCCGGCAGCGTAGGCG‑TTAGGGTCCTTTTGGGCGGAGCGGGATTCGGCGC‑C‑GGAGCCGAGGGCTTTTATGAAGCTGGTCGGGGCGTTGCGGGGGTGGTCAGGGGCGTCGGAAATGGGG‑GGT‑TTG‑GGTT‑G‑CC‑‑ATTTTTTTGCGGG‑CTTGAAAGCGAGATaaa > 1:769972/1‑251 (MQ=255) ttCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTC‑‑GGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGGAAGTAGTTTTTGCCGGCAGCGGAGGCG‑TTAGGTTTATTGTGGGCGGAGCGGTATTTGGCGC‑C‑GAAGCCGAGGGCTTTCATGAAGCTGGTCGGGGGGTTGCGGGGGTGTTCAGGGGCGTC‑GGAATCGGCGGGT‑TTG‑GGTT‑G‑CC‑ATTTTTTTGGGGGT‑TTTG‑AAGCGAGAGaaa > 1:386722/1‑251 (MQ=255) ttCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTC‑‑GGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGGAAGTAGTTTTCGCCGGCAGCGTAGGCG‑TTAGGTTCATTGTGGGCGTAGCGGTATTCGGCGC‑C‑GAAGCCGAGGTCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGa < 1:547383/194‑1 (MQ=255) cAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTC‑‑GGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGGAAGTAGTTTTCGCCGGCAGCGTAGGCG‑TTAGGTTCATTGTGGGCGTAGCGGTATTCGGCGC‑C‑GAAGCCGAGGTCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGACGTC‑GAAATCGGC‑GGA‑TTC‑GGTT‑G‑CC‑AATTTTTTGGCGGC‑TTTGAAAGCGAGATAAACgg < 1:648215/251‑1 (MQ=255) aaCTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTC‑‑GGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGGAAGTAGTTTTTGCCGGCCGCGGAGGCG‑TTTGGTTCCTTTTGGGGGGAGCGGTATTTGGCGC‑C‑GGAGCCGGGGGCTTTTATGAAGCTGGTCGGGGGGTTGCGGGGGTGTTCAGGGGCGTC‑GGAATCGGCGGGA‑TTC‑GGTG‑G‑CC‑AATTTTTTGCGGGC‑TTTGaagagagagaaaaggg > 1:558153/1‑237 (MQ=255) tcCTTAATCTTAATTTCCATGCCTCTGGTGGTC‑‑GGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGGAAGGAGTTTTTGCCGGCCGCGGAGGCG‑TTAGGTTCATTGTGGGCGGAGCGGGATTc > 1:237796/1‑125 (MQ=255) ccTTAATCTTAATTTCCATGCCTCTGGTGGTC‑‑GGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGGAAGGAGTTTTTTCCGGGAGCGTAGGGG‑GTTGGTTTATTTTTGGGGTAGCGGGATTTCGCGC‑CGGAAG‑CGAGGGATTTCATGAAGCTGGTCGGGGGGGTGCGGAGATGTTTAGGGGCGTG‑GGAATAGGG‑GGACTTC‑‑GTT‑G‑CC‑CATTTTTTTGGGGG‑TTTGTAAGCGAGAAAAAAGGCggtgt > 1:664087/1‑245 (MQ=255) ccTTAATCTTAATTTCCATGCCTCTGGTGGTC‑‑GGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGGAAGGAGGTTTTGCCGGCAGCGGAGGGG‑TTAGGGTCATTGTGGGGGGAGCGGGATTCGGCGC‑C‑GAAGCCGAGGGCTTTCATGa > 1:527112/1‑150 (MQ=255) atcttaatTTCCATGCCTCTGGTGGTC‑‑GGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGGAAGTAGTTTTCGCCCGCAGCGTAGGCG‑GTAGGGTCATTGTGGGGGTAGCGGGATTTGGCGC‑C‑GAAGCCGAGGGCTTTTATGAAGCTGGTCGGGGCGTTGCGGGGATTTTTAGGGACGTC‑GAAATCGGC‑GGG‑TTT‑GGGT‑G‑CC‑CATTTTTTTGCGGG‑TTTTGAAGCGGGATAAAACGCGGTGCtttttgg > 1:305971/1‑248 (MQ=255) cttaatTTCCATGCCTCTGGTGGTC‑‑GGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGGAAGTTGTTTTCGCCGGCCGCCTTGGCG‑GTAGGTTCCTTTTGGGCGTTGCGGGATTTGGCGC‑C‑GCAGCCGCGGTCTTTCTTGAAGCTGGTCGGGGGGTTGCGGGGATGTTCACGGACGTC‑GAAATCGGC‑GGG‑TTC‑TGGT‑G‑CCAAATTTTTTTGGGGT‑TTTGAAA‑CGAGATAAACGGCGGTGCTTTTCGGTg > 1:247876/1‑251 (MQ=255) cttaatTTCCATGCCTCTGGTGGTC‑‑GGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGGAAGTAGTTTTTGCCGGCAGCGTAGGCG‑TTAGGTTCATTGTGGGCGGAGCGGGATTCGGCGC‑C‑GAAGCCGAGGGCTTTCATGAAGCTGGTCGGGGCGTTGCGGGGATGTTCAGGGACGTC‑GAAATCGGC‑GGG‑TTC‑GGGT‑G‑CC‑CATTTTTTTGCGGG‑TTTTTAAACGAGATAAACGGCGGTGCTTTTTGGTg > 1:354704/1‑251 (MQ=255) ttCCATGCCTCTGGTGGTC‑‑GGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGGAAGTAGTTTTTGCCGGCAGCGTAGGCG‑TTAGGGTCATTGTGGGCGGAGCGGGATTCGGCGC‑C‑GAAGCCGAGGGCTTTTATGAAGCTGGTCGGGGCGTTGCGGGGATGTTCAGGGACGTC‑GAAATCGGC‑GGG‑TTC‑GGTT‑G‑CC‑AATTTTTTTGCGGG‑TTTTAAAGCGAGATAAAAGGCGGTGCttttttgggcggcgg > 1:173980/1‑240 (MQ=255) gCCTCTGGTGGTC‑‑GGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGGAAGGAGTTTTTGCCGGCAGCGGAGGCG‑TTTGGTTTATTGTGGGGGTAGCGGGATTCGGCGC‑C‑GAAGCCGAGGGCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGACGTC‑GGAATCGGC‑GGA‑TTCGGGAT‑G‑‑C‑AATTTTTTTGGGGC‑TTTTGAAGCGAGGAAAAAGGCGGTGCTTTTTGGGGCGACGGCttaaa > 1:799798/1‑247 (MQ=255) ccTCTGGTGGTC‑‑GGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGGGAGGAGTTTTCGCCGGCAGCGGAGGGG‑GTAGGGTCATTTTGGGCGTAGCGGGATTCGGCGC‑C‑GAAGCCGAGGGCTTTCATGAAGCTGGTCGGGGCGGTGCGGGGATGTTTAGGGGCGTC‑GAAATCGGCGGGT‑TTG‑GGTT‑C‑CC‑ATTTTTTTGG‑GGG‑TTTGGAAGGGAGAGAAAAGGGGTTGCTTTTTGGTGGGACGGGTAAATa > 1:784936/1‑251 (MQ=255) ccTCTGGTGGTC‑‑GGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGGAAGTAGTTTTCGCCGGCAGCGTAGGCG‑TTAGGTTCATTGTGGGCGTAGCGGTATTCGGCGC‑C‑GAAGCCGAGGTCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGACGTC‑GAAATCGGC‑GGA‑TTC‑GGTT‑G‑CC‑AATTTTTTGGCGGC‑TTTGAAAGCGAGATAAACGGCGt < 1:549226/226‑1 (MQ=255) ctctGGTGGTC‑‑GGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGGAAGTAGTTTTCGCCGGCAGCGTAGGCG‑TTAGGTTCATTGTGGGCGTAGCGGTATTCGGCGC‑C‑GAAGCCGAGGTCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGACGTC‑GAAATCGGC‑GGA‑TTC‑GGTT‑G‑CC‑AATTTTTTGGCGGC‑TTTGAAAGCGAGATAAACGGCGTTGCTTTTCGGTGCGACGGCTAAATac < 1:618094/251‑1 (MQ=255) ctGGTGGTC‑‑GGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGGAAGTAGTTTTCGCCGGCAGCGTAGGCG‑TTAGGTTCATTGTGGGCGTAGCGGTATTCGGCGC‑C‑GAAGCCGAGGTCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGACGTC‑GAAATCGGC‑GGA‑TTC‑GGTT‑G‑CC‑AATTTTTTGGCGGC‑TTTGAAAGCGAGATAAACGGCGTTGCTTTTCGGTGCGACGGCTAAATacac < 1:365910/251‑1 (MQ=255) tggtggTC‑‑GGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGGAAGTAGTTTTCGCCGGCAGCGTAGGCG‑TTAGGTTCATTGTGGGCGTAGCGGTATTCGGCGC‑C‑GAAGCCGAGGTCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGACGTC‑GAAATCGGC‑GGA‑TTC‑GGTT‑G‑CC‑AATTTTTTGGCGGC‑TTTGAAAGCGAGATAAACGGCGTTGCTTTTCGGTGCGACGGCTAAATAcaca < 1:120036/251‑1 (MQ=255) tC‑‑GGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGGAAGTAGTTTTCGCCGGCAGCGTAGGCG‑TTAGGTTCATTGTGGGCGTAGCGGTATTCGGCGC‑C‑GAAGCCGAGGTCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGACGTC‑GAAATCGGC‑GGA‑TTC‑GGTT‑G‑CC‑AATTTTTTGGCGGC‑TTTGAAAGCGAGATAAACGGCGTTGCTTTTCGGTGCGACGGCTAAATACACAATGGc < 1:417777/250‑1 (MQ=255) tC‑‑GGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGGAAGTAGTTTTCGCCGGCAGCGTAGGCG‑TTAGGTTCATTGTGGGCGTAGCGGTATTCGGCGC‑C‑GAAGCCGAGGTCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGACGTC‑GAAATCGGC‑GGA‑TTC‑GGTT‑G‑CC‑AATTTTTTGGCGGC‑TTTGAAAGCGAGATAAACGGCGTTGCTTTTCGGTGCGACGGCTAAATACACAATGGCt < 1:477960/251‑1 (MQ=255) tC‑‑GGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGGAAGTAGTTTTCGCCGGCAGCGTAGGCG‑TTAGGTTCATTGTGGGCGTAGCGGTATTCGGCGC‑C‑GAAGCCGAGGTCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGACGTC‑GAAATCGGC‑GGA‑TTC‑GGTT‑G‑CC‑AATTTTTTGGCGGC‑TTTGAAAGCGAGATAAACGGCGTTGCTTTTCGGTGCGACGGCTAAATACACAATGGCt < 1:355561/251‑1 (MQ=255) ggAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGGAAGTAGTTTTTGCCGGCCGCGGAGGCG‑GTAGGTTCATTGTGGGCGTAGCGGGATTCGGCGC‑C‑GCAGCCGAGGGCTTTCATGAAGCTGGTCGGGGCGGTGCGGAGATGTTCAGGGACGTC‑GAAATCGGC‑GGG‑TTCGGGTG‑G‑CC‑ATTTTTTTGG‑GGG‑TTTGTAAGCGAGATAAACGGCGGTGCTTTTTGGGGCGGCGGCTAAAtctgtctcttattaa > 1:170260/1‑237 (MQ=255) aaaTAGAATTGGGTGTCTTTCAGTTGTGGCGGGAAGTAGTTTTTGCCGGCAGCGGAGGCG‑TTAGGTTCCTTGTGGGCGGAGCGGTATTTGGCGC‑C‑GGAGCCGAGGGCTTTCATGAAGCTGGTCGGGGCGGTGCGGAGGTGTTCAGGGACGTC‑GAAATCGGC‑GGA‑TTC‑GGTT‑GCCC‑ATTTTTTTGGGGGT‑TTTG‑AAGCGGGGTAAACCGCGGTGCTTTTTGGTGGGGCGGGTAAATACACAAAGGGTTgcg > 1:721207/1‑250 (MQ=255) aaaTAGAATTGGGTGTCTTTCAGTTGTGGCGGGAAGTAGTTTTCGCCGGCAGCGTAGGCG‑TTAGGTTCATTGTGGGCGTAGCGGTATTCGGCGC‑C‑GAAGCCGAGGTCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGACGTC‑GAAATCGGC‑GGA‑TTC‑GGTT‑G‑CC‑AATTTTTTGGCGGC‑TTTGAAAGCGAGATAAACGGCGTTGCTTTTCGGTGCGACGGCTAAATACACAATGGCTTgcgc < 1:609350/251‑1 (MQ=255) aaaTAGAATTGGGTGTCTTTCAGTTGTGGCGGGAAGGAGTTTTTGCCGGCAGCGGAGGCG‑TTAGGGTCATTGTGGGCGTAGGGGGATTCGGCGC‑C‑GAAGCCGAGGGCTTTCATGAAGCTGGTCGGGGGGTTGCGGGGGTGGTTAGGGACGTC‑GAAATCGGA‑GGA‑GTT‑GGTT‑G‑CCAAATTTTTTGG‑GGCTTTTGAAAG‑GAGATAAACGGCGGTGCTTTTCCGTGCGACGGCTAAATaaaaaaaggcgttgg > 1:600/1‑236 (MQ=255) gAATTGGGTGTCTTTCAGTTGTGGCGGGAAGGAGTTTTCGCCGGCAGCGGAGGCG‑TTAGGGTCATTGTGGGGGTAGCGGGATTCGGCGC‑C‑GAAGCCGAGGGCTTTTCTGAAGCTGGTCGGGGCGGTGCGGGGATGTTCAGGGGCGGC‑GGAATCGGC‑GGG‑GTT‑GGTTGG‑CC‑ATTTTTTTGG‑GGC‑TTTTAAAGAGAGATAAAAGGCGGTTCTTTTTGGTGCGGCGGGTTAATAAACaaatgctttcggaaaaa > 1:642386/1‑236 (MQ=255) aaTTGGGTGTCTTTCAGTTGTGGCGGGAAGTAGTTTTCGCCGGCAGCGTAGGCG‑TTAGGTTCATTGTGGGCGTAGCGGTATTCGGCGC‑C‑GAAGCCGAGGTCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGACGTC‑GAAATCGGC‑GGA‑TTC‑GGTT‑G‑CC‑AATTTTTTGGCGGC‑TTTGAAAGCGAGATAAACGGCGTTGCTTTTCGGTGCGACGGCTAAATACACAATGGCTTGCGCAATAg < 1:813625/250‑1 (MQ=255) aaTTGGGTGTCTTTCAGTTGTGGCGGGAAGTAGTTTTCGCCGGCAGCGTAGGCG‑TTAGGTTCATTGTGGGCGTAGCGGTATTCGGCGC‑C‑GAAGCCGAGGTCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGACGTC‑GAAATCGGC‑GGA‑TTC‑GGTT‑G‑CC‑AATTTTTTGGCGGC‑TTTGAAAGCGAGATAAACGGCGTTGCTTTTCGGTGCGACGGCTAAATACACAATGGCTTGCGCAATAg < 1:269845/250‑1 (MQ=255) aaTTGGGTGTCTTTCAGTTGTGGCGGGAAGTAGTTTTCGCCGGCAGCGTAGGCG‑TTAGGTTCATTGTGGGCGTAGCGGTATTCGGCGC‑C‑GAAGCCGAGGTCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGACGTC‑GAAATCGGC‑GGA‑TTC‑GGTT‑G‑CC‑AATTTTTTGGCGGC‑TTTGAAAGCGAGATAAACGGCGTTGCTTTTCGGTGCGACGGCTAAATACACAATGGCTTGCGCAATAg < 1:467917/250‑1 (MQ=255) ttGGGTGTCTTTCAGTTGTGGCGGGAAGTAGTTTTCGCCGGCAGCGTAGGCG‑TTAGGTTCATTGTGGGCGTAGCGGTATTCGGCGC‑C‑GAAGCCGAGGTCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGACGTC‑GAAATCGGC‑GGA‑TTC‑GGTT‑G‑CC‑AATTTTTTGGCGGC‑TTTGAAAGCGAGATAAACGGCGTTGCTTTTCGGTGCGACGGCTAAATACACAATGGCTTGCGCAATAgcgc < 1:796820/251‑1 (MQ=255) gtCTTTCAGTTGTGGCGGGAAGTAGTTTTCGCCGGCAGCGTAGGCG‑TTAGGTTCATTGTGGGCGTAGCGGTATTCGGCGC‑C‑GAAGCCGAGGTCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGACGTC‑GAAATCGGC‑GGA‑TTC‑GGTT‑G‑CC‑AATTTTTTGGCGGC‑TTTGAAAGCGAGATAAACGGCGTTGCTTTTc < 1:641804/205‑1 (MQ=255) gtCTTTCAGTTGTGGCGGGAAGTAGTTTTCGCCGGCAGCGTAGGCG‑TTAGGTTCATTGTGGGCGGAGCGGTATTCGGCGC‑C‑GAAGCCGAGGGCTTTCATGAAGCTGGTCGGGGCGTTGCGGGGATGTTCAGGGACGTC‑GAAATCGGC‑GGG‑TTC‑GGGT‑G‑CC‑CATTTTTTTGGGGG‑TTTTTAAGCGGGATAAAAGGGGGTGCTTTTTGGTGCGGCGGGTAAATAAACAAAGGCTTGCGCCAAAACGCGTTCg > 1:190833/1‑250 (MQ=255) gtCTTTCAGTTGTGGCGGGAAGTAGTTATCGCCGGCAGCGTAGGCG‑TTAGGTTCAATGTGGGCGTAGCGGTATTCGGCGC‑C‑GAAGCCGAGGTCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGACGTC‑GAAATCGGC‑GGA‑TTC‑GGTT‑G‑CC‑AATTTTTTGGCGGC‑TTTGAAAGCGAGATAAACGGCGTTGCTTTTCGGTGCGACGGCTAAATac < 1:625721/223‑1 (MQ=255) tCTTTCAGTTGTGGCGGGAAGTAGTTTTCGCCGGCAGCGTAGGCG‑TTAGGTTCATTGTGGGCGTAGCGGTATTCGGCGC‑C‑GAAGCCGAGGTCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGACGTC‑GAAATCGGC‑GGA‑TTC‑GGTT‑G‑CC‑AATTTTTTGGCGGC‑TTTGAAAGCGAGATAAACGGCGTTGCTTTTCGGTGCGACGGCTAAATac < 1:211754/222‑1 (MQ=255) ttGTGGCGGGAAGTAGTTTTCGCCGGCAGCGTAGGCG‑TTAGGTTCATTGTGGGCGTAGCGGTATTCGGCGC‑C‑GAAGCCGAGGTCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGACGTC‑GAAATCGGC‑GGA‑TTC‑GGTT‑G‑CC‑AATTTTTTGGCGGC‑TTTGAAAGCGAGATAAACGGCGTTGCTTTTCGGTGCGACGGCTAAATACACAATGGCTTGCGCAATAGCGCGTTCGCCTTCGGc < 1:160625/249‑1 (MQ=255) gtgGCGGGAAGTAGTTTTCGCCGGCAGCGTAGGCG‑TTAGGTTCATtg > 1:610553/1‑47 (MQ=255) gtgGCGGGAAGTAGTTTTCGCCGGCAGCGTAGGCG‑TTAGGTTCATTGTGGGCGTAGCGGTATTCGGCGC‑C‑GAAGCCGAGGTCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGACGTC‑GAAATCGGC‑GGA‑TTC‑GGTT‑G‑CC‑AATTTTTTGGCGGC‑TTTGAAAGCGAGATAAACGGCGTTGCTTTTCGGTGCGACGGCTAAATACACAATGGCTTGCGCAATAGCGCGTTCGCCTTCGGCAgcg > 1:659596/1‑251 (MQ=255) gAAGTAGTTTTCGCCGGCAGCGTAGGCG‑TTAGGTTCATTGTGGGCGTAGCGGTATTCGGCGC‑C‑GAAGCCGAGGTCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGACGTC‑GAAATCGGC‑GGA‑TTC‑GGTT‑G‑CC‑AATTTTTTGGCGGC‑TTTGAAAGCGAGATAAACGGCGTTGCTTTTCGGTGCGACGGCTAAATACACAATGGCTTTCGCAATAGCGCGTTCGCCTTCGGCAGCGCCGAcg > 1:209371/1‑250 (MQ=255) | AAGACAAAATCCAAGGATTTTGAACGTTGGCTTTGCCAATGGTACGAAGTGCAAGCGCAGCGCAGTAAAAGTAGGTCGATGCCGAAACAAACCTATTTATATCGCTTCTTACCACTTTCCTGATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTC‑‑GGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGGAAGTAGTTTTCGCCGGCAGCGTAGGCG‑TTAGGTTCATTGTGGGCGTAGCGGTATTCGGCGC‑C‑GAAGCCGAGGTCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGACGTC‑GAAATCGGC‑GGA‑TTC‑GGTT‑G‑CC‑AATTTTTTGGCGGC‑TTTGAAAGCGAGATAAACGGCGTTGCTTTTCGGTGCGACGGCTAAATACACAATGGCTTGCGCAATAGCGCGTTCGCCTTCGGCAGCGCCGACG > 1/1973068‑1973535 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |