Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,847,175	G→A	intergenic (+12734/‑137850)	unknown → / → unknown	tRNA‑Leu‑CAA/tRNA‑Asp‑GTC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,847,175	0	G	A	100.0%	284.7 / NA	80	intergenic (+12734/‑137850)	unknown/unknown	tRNA‑Leu‑CAA/tRNA‑Asp‑GTC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (48/32);  total (48/32)

GTCTGCGCCCGCTTGTTCAATAAAAATTCACATTCATTTTCCACCACACTTTGCCACTCGGTTTGCGTAAGCTGTTGTTGCATTTGTTCATATAACGGCAACAAATACCATACATCCGCCGCCGCATATTGCAGCTGCGTATCCGATAACGGGCGTGCCAGCCAATCGGTACGGGAGGCGCCTTTGTCGATTTCAATTTGGAAATAATGTTGCACCAAGGTAGCGAAACCGGTGGAGCCGGCGAAGCCGAGAAAATGCGCCATCACTTGTGTATCACACATCGGGCGCGGCAGCTGCTGAAAATAATGCTGGAATACGTCTAAATCTTCGCTACAAGCATGCAGAATTTTGACCAAATTAGTATCTGCAAGCAATTTAATGAACGGCGAAAAATCCTGAATCGGTAAGGGATCGATTAACGCCACCCGATCGCCGAAATACATCTGAATTAAACCGAGTTTCGGATACAGGGTTCGTAC  >  1/1846933‑1847411                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                             gTCTGCGCCCGCTTGTTCAATAAAAATTCACATTCATTTTCCACCACACTTTGCCACTCGGTTTGCGTAAGCTGTTGTTGCATTTGTTCATATAACGGCAACAAATACCATACATCCGCCGCCGCATATTGCAGCTGCGTATCCGATAACGGGCGTGCCAGCCAATCGGTACGGGAGGCGCCTTTGTCGATTTCAATTTGGAAATAATGTTGCACCAAGGTAGCGAAACCGGTGGAGCCGGCAAAGc                                                                                                                                                                                                                                          >  1:618635/1‑247 (MQ=255) gTCTGCGCCCGCTTGTTCAATAAAAATTCACATTCATTTTCCACCACACTTTGCCACTCGGTTTGCGTAAGCTGTTGTTGCATTTGTTCATATAACGGCAACAAATACCATACATCCGCCGCCGCATATTGCAGCTGCGTATCCGATAACGGGCGTGCCAGCCAATCGGTACGGGAGGCGCCTTTGTCGATTTCAATTTGGAAATAATGTTGCACCAAGGTAGCGAAACCGGTGGAGCCGGCAAAGCCgag                                                                                                                                                                                                                                      >  1:367998/1‑251 (MQ=255)     gcgcCCGCTTGTTCAATAAAAATTCACATTCATTTTCCACCACACTTTGCCACTCGGTTTGCGTAAGCTGTTGTTGCATTCGTTCATATAACGGCAACAAATACCATACATCCGCCGCCGCATATTGCAGCTGCGTATCCGATAACGGGCGTGCCAGCCAATCGGTACGGGAGGCGCCTTTGTCGATTTCAATTTGGAAATAATGTTGCACCAAGGTAGCGAAACCGGTGGAGCCGGCAAAGCCGAGaaa                                                                                                                                                                                                                                   <  1:445280/250‑1 (MQ=255)          cGCTTGTTCAATAAAAATTCACATTCATTTTCCACCACACTTTGCCACTCGGTTTGCGTAAGCTGTTGTTGCATTTGTTCATATAACGGCAACAAATACCATACATCCGCCGCCGCATATTGCAGCTGCGTATCCGATAACGGGCGTGCCAGCCAATCGGTACGGGAGGCGCCTTTGTCGATTTCAATTTGGAAATAATGTTGCACCAAGGTAGCGAAACCGGTGGAGCCGGCAAAGCCGAGAAAATgcg                                                                                                                                                                                                                              >  1:394145/1‑250 (MQ=255)            cTTGTTCAATAAAAATTCACATTCATTTTCCACCACACTTTGCCACTCGGTTTGCGTAAGCTGTTGTTGCATTTGTTCATATAACGGCAACAAATACCATACATCCGCCGCCGCATATTGCAGCTGCGTATCCGATAACGGGCGTGCCAGCCAATCGGTACGGGAGGCGCCTTTGTCGATTTCAATTTGGAAATAATGTTGCACCAAGGTAGCGAAACCGGTGGAGCCGGCAAAGCCGAGAAAATGCGCCa                                                                                                                                                                                                                           >  1:596470/1‑251 (MQ=255)                  cAATAAAAATTCACATTCATTTTCCACCACACTTTGCCACTCGGTTTGCGTAAGCTGTTGTTGCATTTGTTCATATAACGGCAACAAATACCATACATCCGCCGCCGCATATTGCAGCTGCGTATCCGATAACGGGCGTGCCAGCCAATCGGTACGGGAGGCGCCTTTGTCGATTTCAATTTGGAAATAATGTTGCACCAAGGTAGCGAAACCGGTGGAGCCGGCAAAGCCGAGAAAATGCGCCATCACtt                                                                                                                                                                                                                     >  1:815898/1‑251 (MQ=255)                    aTAAAAATTCACATTCATTTTCCACCACACTTTGCCACTCGGTTTGCGTAAGCTGTTGTTGCATTTGTTCATATAACGGCAACAAATACCATACATCCGCCGCCGCATATTGCAGCTGCGTATCCGATAACGGGCGTGCCAGCCAATCGGTACGGGAGGCGCCTTTGTCGATTTCAATTTGGAAATAATGTTGCACCAAGGTAGCGAAACCGGTGGAGCCGGCAAAGCCGAGAAAATGCGCCATCACTtgt                                                                                                                                                                                                                   >  1:128098/1‑251 (MQ=255)                      aaaaaTTCACATTCATTTTCCACCACACTTTGCCACTCGGTTTGCGTAAGCTGTTGTTGCATTTGTTCATATAACGGCAACAAATACCATACATCCGCCGCCGCATATTGCAGCTGCGTATCCGATAACGGGCGTGCCAGCCAATCGGTACGGGAGGCGCCTTTGTCGATTTCAATTTGGAAATAATGTTGCACCAAGGTAGCGAAACCGGTGGAGCCGGCAAAGCCGAGAAAATGCGCCATCACTTgtgt                                                                                                                                                                                                                 <  1:537944/251‑1 (MQ=255)                           ttCACATTCATTTTCCACCACACTTTGCCACTCGGTTTGCGTAAGCTGTTGTTGCATTTGTTCATATAACGGCAACAAATACCATACATCCGCCGCCGCATATTGCAGCTGCGTATCCGATAACGGGCGTGCCAGCCAATCGGTACGGGAGGCGCCTTTGTCGATTTCAATTTGGAAATAATGTTGCACCAAGGTAGCGAAACCGGTGGAGCCGGCAAAGCCGAGAAAATGCGCCATCACTTGTGTATcac                                                                                                                                                                                                            <  1:267490/251‑1 (MQ=255)                             cacaTTCATTTTCCACCACACTTTGCCACTCGGTTTGCGTAAGCTGTTGTTGCATTTGTTCATATAACGGCAACAAATACCATACATCCGCCGCCGCATATTGCAGCTGCGTATCCGATAACGGGCGTGCCAGCCAATCGGTACGGGAGGCGCCTTTGTCGATTTCAATTTGGAAATAATGTTGCACCAAGGTAGCGAAACCGGTGGAGCCGGCAAAGCCGAGAAAATGCGCCATCACTTGTGTATcacac                                                                                                                                                                                                          <  1:111594/251‑1 (MQ=255)                               cattcattTTCCACCACACTTTGCCACTCGGTTTGCGTAAGCTGTTGTTGCATTTGTTCATATAACGGCAACAAATACCATACATCCGCCGCCGCATATTGCAGCTGCGTATCCGATAACGGGCGTGCCAGCCAATCGGTACGGGAGGCGCCTTTGTCGATTTCAATTTGGAAATAATGTTGCACCAAGGTAGCGAAACCGGTGGAGCCGGCAAAGCCGAGAAAATGCGCCATCACTTGTGTATCACACAt                                                                                                                                                                                                        >  1:23925/1‑251 (MQ=255)                                         ccaccaCACTTTGCCACTCGGTTTGCGTAAGCTGTTGTTGCATTTGTTCATATAACGGCAACAAATACCATACATCCGCCGCCGCATATTGCAGCTGCGTATCCGATAACGGGCGTGCCAGCCAATCGGTACGGGAGGCGCCTTTGTCGATTTCAATTTGGAAATAATGTTGCACCAAGGTAGCGAAACCGGTGGAGCCGGCAAAGCCGAGAAAATGCGCCATCACTTGTGTATCACACATCGGGCGCgg                                                                                                                                                                                               >  1:192081/1‑250 (MQ=255)                                                     gCCACTCGGTTTGCGTAAGCTGTTGTTGCATTTGTTCATATAACGGCAACAAATACCATACATCCGCCGCCGCATATTGCAGATGCGTATCCGATAACGGGCGTGCCAGCCAATCGGTACGGGAGGCGCCTTTGTCGATTTCAATTTGGAAATAATGTTGCACCAAGGTAGCGAAACCGGTGGAGCCGGCAAAGCCGAGAAAATGCGCCATCACTTGTGTATCACACATCGGGCGCGGCAGCTGCTGaaaa                                                                                                                                                                                  >  1:770216/1‑251 (MQ=255)                                                             gTTTGCGTAAGCTGTTGTTGCATTTGTTCATATAACGGCAACAAATACCATACATCCGCCGCCGCATATTGCAGCTGCGTATCCGATAACGGGCGTGCCAGCCAATCGGTACGGGAGGCGCCTTTGTCGATTTCAATTTGGAAATAATGTTGCACCAAGGTAGCGAAACCGGTGGAGCCGGCAAAGCCGAGAAAATGCGCCATCACTTGTGTATCACACAt                                                                                                                                                                                                        >  1:599835/1‑221 (MQ=255)                                                             gTTTGCGTAAGCTGTTGTTGCATTTGTTCATATAACGGCAACAAATACCATACATCCGCCGCCGCATATTGCAGCTGCGTATCCGATAACGGGCGTGCCAGCCAATCGGTACGGGAGGCGCCTTTGTCGATTTCAATTTGGAAATAATGTTGCACCAAGGTAGCGAAACCGGTGGAGCCGGCAAAGCCGAGAAAATGCGCCATCACTTGTGTATCACACATCGGGCGCGGCAGCTGCTGAAaat                                                                                                                                                                                 <  1:132367/244‑1 (MQ=255)                                                                 gcggaaGCTGTTGTTGCATTTGTTCATATAACGGCAACAAATACCATACATCCGCCGCCGCATATTGCAGCTGCGTATCCGATAACGGGCGTGCCAGCCAATCGGTACGGGAGGCGCCTTTGTCGATTTCAATTTGGAAATAATGTTGCACCAAGGTAGCGAAACCGGTGGAGCCGGCAAAGCCGAGAAAATGCGCCATCACTTGTGTATCACACATCGGGCGCGGCAGCTGCTGAAAATAATGCTGGAAt                                                                                                                                                                      >  1:757252/5‑251 (MQ=255)                                                                     aaGCTGTTGTTGCATTTGTTCATATAACGGCAACAAATACCATACATCCGCCGCCGCATATTGCAGCTGCGTATCCGATAACGGGCGTGCCAGCCAATCGGTACGGGAGGCGCCTTTGTCGATTTCAATTTGGAAATAATGTTGCACCAAGGTAGCGAAACCGGTGGAGCCGGCAAAGCCGAGAAAATGCGCCATCACTTGTGTATCACACATCGGGCGCGGCAGCTGCTGAAAATAATGCTGGAATACGt                                                                                                                                                                  >  1:16899/1‑251 (MQ=255)                                                                        cTGTTGTTGCATTTGTTCATATAACGGCAACAAATACCATACATCCGCCGCCGCATATTGCAGCTGCGTATCCGATAACGGGCGTGCCAGCCAATCGGTACGGGAGGCGCCTTTGTCGATTTCAATTTGGAAATAATGTTGCACCAAGGTAGCGAAACCGGTGGAGCCGGCAAAGCCGAGaaa                                                                                                                                                                                                                                   <  1:58871/183‑1 (MQ=255)                                                                        cTGTTGTTGCATTTGTTCATATAACGGCAACAAATACCATACATCCGCCGCCGCATATTGCAGCTGCGTATCCGATAACGGGCGTGCCAGCCAATCGGTACGGGAGGCGCCTTTGTCGATTTCAATTTGGAAATAATGTTGCACCAAGGTAGCGAAACCGGTGGAGCCGGCAAAGCCGAGAAAATGCGCCATCACTTGTGTATCACACATCGGGCGCGGCAGCTGCTGAAAATAATGCTGGAATACGTCTa                                                                                                                                                               >  1:468522/1‑251 (MQ=255)                                                                          gttgttGCATTTGTTCATATAACGGCAACAAATACCATACATCCGCCGCCGCATATTGCAGCTGCGTATCCGATAACGGGCGTGCCAGCCAATCGGTACGGGAGGCGCCTTTGTCGATTTCAATTTGGAAATAATGTTGCACCAAGGTAGCGAAACCGGTGGAGCCGGCAAAGCCGAGAAAATGCGCCATCACTTgtgt                                                                                                                                                                                                                 <  1:456542/199‑1 (MQ=255)                                                                                      gTTCATATAACGGCAACAAATACCATACATCCGCCGCCGCATATTGCAGCTGCGTATCCGATAACGGGCGTGCCAGCCAATCGGTACGGGAGGCGCCTTTGTCGATTTCAATTTGGAAATAATGTTGCACCAAGGTAGCGAAACCGGTGGAGCCGGCAAAGc                                                                                                                                                                                                                                          >  1:240194/1‑162 (MQ=255)                                                                                      gTTCATATAACGGCAACAAATACCATACATCCGCCGCCGCATATTGCAGCTGCGTATCCGATAACGGGCGTGCCAGCCAATCGGTACGGGAGGCGCCTTTGTCGATTTCAATTTGGAAATAATGTTGCACCAAGGTAGCGAAACCGGTGGAGCCGGCAAAGCCGAGAAAATGCGCCATCACTTGTGTATcac                                                                                                                                                                                                            <  1:241706/192‑1 (MQ=255)                                                                                      gTTCATATAACGGCAACAAATACCATACATCCGCCGCCGCATATTGCAGCTGCGTATCCGATAACGGGCGTGCCAGCCAATCGGTACGGGAGGCGCCTTTGTCGATTTCAATTTGGAAATAATGTTGCACCAAGGTAGCGAAACCGGTGGAGCCGGCAAAGCCGAGAAAATGCGCCATCACTTGTGTATcac                                                                                                                                                                                                            >  1:471375/1‑192 (MQ=255)                                                                                         cATATAACGGCAACAAATACCATACATCCGCCGCCGCATATTGCAGCTGCGTATCCGATAACGGGCTTGCCAGCCAATCGCTACGGGAGGCGCCTTTGTCGATTTCAATTTGGAAATAATGTTGCACCAATGTAGCGAAACCGGTGGAGCCGGCAAAGCCGAGAAAATGCGCCATCACTTGTGTATCACACATCGGGCGCGGCAGCTGCTGAAAATAATGCTGGAATACGTCTACATCTTCGCTACAAGCa                                                                                                                                              >  1:890782/1‑251 (MQ=255)                                                                                         cATATAACGGCAACAAATACCATACATCCGCCGCCGCATATTGCAGCTGCGTATCCGATAACGGGCGTGCCAGCCAATCGGTACGGGAGGCGCCTTTGTCGATTTCAATTTGGAAATAATGTTGCACCAAGGTAGCGAAACCGGTGGAGCCGGCAAAGCCGAGAAAATGCGCCATCACTTGTGTATCACACATCGGGCGCGGCAGCTGCTGAAAATAATGCTGGAATACGTCTAAATCTTCGCTACAAGCa                                                                                                                                              <  1:141653/251‑1 (MQ=255)                                                                                           tataACGGCAACAAATACCATACATCCGCCGCCGCATATTGCAGCTGCGTATCCGATAACGGGCGTGCCAGCCAATCGGTACGGGAGGCGCCTTTGTCGATTTCAATTTGGAAATAATGTTGCACCAAGGTAGCGAAACCGGTGGAGCCGGCAAAGCCGAGAAAATGCGCCATCACTTGTGTATCACACATCGGGCGCGGCAGCTGCTGAAaat                                                                                                                                                                                 <  1:270441/214‑1 (MQ=255)                                                                                                 ggCAACAAATACCATACATCCGCCGCCGCATATTGCAGCTGCGTATCCGATAACGGGCGTGCCAGCCAATCGGTACGGGAGGCGCCTTTGTCGATTTCAATTTGGAAATAATGTTGCACCAAGGTAGCGAAACCGGTGGAGCCGGCAAAGCCGAGAAAATGCGCCATCACTTGTGTAt                                                                                                                                                                                                               <  1:523479/178‑1 (MQ=255)                                                                                                  gCAACAAATACCATACATCCGCCGCCGCATATTGCAGCTGCGTATCCGATAACGGGCGTGCCAGCCAATCGGTACGGGAGGCGCCTTTGTCGATTTCAATTTGGAAATAATGTTGCACCAAGGTAGCGAAACCGGTGGAGCCGGCAAAGCCGAGAAAATGCGCCATCACTTGTGTATCACACATCGGGCGCGGCAGCTGCTGAAaat                                                                                                                                                                                 >  1:689110/1‑207 (MQ=255)                                                                                                  gCAACAAATACCATACATCCGCCGCCGCATATTGCAGCTGCGTATCCGATAACGGGCGTGCCAGCCAATCGGTACGGGAGGCGCCTTTGTCGATTTCAATTTGGAAATAATGTTGCACCAAGGTAGCGAAACCGGTGGAGCCGGCAAAGCCGAGAAAATGCGCCATCACTTGTGTATCACACATCGGGCGCGGCAGCTGCTGAAAATAATGCTGGaa                                                                                                                                                                       >  1:561475/1‑217 (MQ=255)                                                                                                   caacaaATACCATACATCCGCCGCCGCATATTGCAGCTGCGTATCCGATAACGGGCGTGCCAGCCAATCGGTACGGGAGGCGCCTTTGTCGATTTCAATTTGGAAATAATGTTGCACCAAGGTAGCGAAACCGGTGGAGCCGGCAAAGCCGAGAAAATGCGCCATCACTTGTGTATcacac                                                                                                                                                                                                          >  1:89163/1‑181 (MQ=255)                                                                                                   caacaaATACCATACATCCGCCGCCGCATATTGCAGCTGCGTATCCGATAACGGGCGTGCCAGCCAATCGGTACGGGAGGCGCCTTTGTCGATTTCAATTTGGAAATAATGTTGCACCAAGGTAGCGAAACCGGTGGAGCCGGCAAAGCCGAGAAAATGCGCCATCACTTGTGTATCACACATCGGGCGCGGCAGCTGCTGAAAATAATGCTGGAATACGTCTAAATCTTCGCTACAAGCATGCAGAAttt                                                                                                                                    <  1:85532/251‑1 (MQ=255)                                                                                                    aacaaATACCATACATCCGCCGCCGCATATTGCAGCTGCGTATCCGATAACGGGCGTGCCAGCCAATCGGTACGGGAGGCGCCTTTGTCGATTTCAATTTGGAAATAATGTTGCACCAAGGTAGCGAAACCGGTGGAGCCGGCAAAGCCGAGAAAATGCGCCATCACTTGTGTATCACACATCGGGCGCGGCAGCTGCTGAAAATAATGCTGGAATACGTCTAAATCTTCGCTACAAGCATGCAGAAtttt                                                                                                                                   >  1:774665/1‑251 (MQ=255)                                                                                                     acaaATACCATACATCCGCCGCCGCATATTGCAGCTGCGTATCCGATAACGGGCGTGCCAGCCAATCGGTACGGGAGGCGCCTTTGTCGATTTCAATTTGGAAATAATGTTGCACCAAGGTAGCGAAACCGGTGGAGCCGGCAAAGCCGAGAAAATGCGCCATCACTTGTGTATcacac                                                                                                                                                                                                          <  1:591942/179‑1 (MQ=255)                                                                                                         aTACCATACATCCGCCGCCGCATATTGCAGCTGCGTATCCGATAACGGGCGTGCCAGCCAATCGGTACGGGAGGCGCCTTTGTCGATTTCAATTTGGAAATAATGTTGCACCAAGGTAGCGAAACCGGTGGAGCCGGCAAAGCCGAGaaa                                                                                                                                                                                                                                   <  1:182041/150‑1 (MQ=255)                                                                                                                  aTCCGCCGCCGCATATTGCAGCTGCGTATCCGATAACGGGCGTGCCAGCCAATCGGTACGGGAGGCGCCTTTGTCGATTTCAATTTGGAAATAATGTTGCACCAAGGTAGCGAAACCGGTGGAGCCGGCAAAGCCGAGAAAATGCGCCATCACTTGTGTATCACACATCGGGCGCGGCAGCTGCTGAAAATAATGCTGGAATACGTCTAAATCTTCGCTACAAGCATGCAGAATTTTGACCAAATTAGTAt                                                                                                                     <  1:71090/251‑1 (MQ=255)                                                                                                                  aTCCGCCGCCGCATATTGCAGCTGCGTATCCGATAACGGGCGTGCCAGCCAATCGGTACGGGAGGCGCCTTTGTCGATTTCAATTTGGAAATAATGTTGCACCAAGGTAGCGAAACCGGTGGAGCCGGCAAAGCCGAGAAAATGCGCCATCACTTGTGTATCACACATCGGGCGCGGCAGCTGCTGAAAATAATGCTGGAATACGTCTAAATCTTCGCTACAAGCATGCAGAATTTTGACCAAATTAGTAt                                                                                                                     <  1:158747/251‑1 (MQ=255)                                                                                                                   tCCGCCGCCGCATATTGCAGCTGCGTATCCGATAACGGGCGTGCCAGCCAATCGGTACGGGAGGCGCCTTTGTCGATTTCAATTTGGAAATAATGTTGCACCAAGGTAGCGAAACCGGTGGAGCCGGCAAAGCCGAGAAAATGCGCCATCACTTGTGTATCACACATCGGGCGCGGCAGCTGCTGAAAATAATGCTGGAATACGTCTAAATCTTCGCTACAAGCATGCAGAATTTTGACCAAATTAGTATc                                                                                                                    <  1:542981/251‑1 (MQ=255)                                                                                                                       ccgccgcATATTGCAGCTGCGTATCCGATAACGGGCGTGCCAGCCAATCGGTACGGGAGGCGCCTTTGTCGATTTCAATTTGGAAATAATGTTGCACCAAGGTAGCGAAACCGGTGGAGCCGGCAAAGCCGAGAAAATGCGCCATCACTtgtg                                                                                                                                                                                                                  <  1:585891/153‑1 (MQ=255)                                                                                                                       ccgccgcATATTGCAGCTGCGTATCCGATAACGGGCGTGCCAGCCAATCGGTACGGGAGGCGCCTTTGTCGATTTCAATTTGGAAATAATGTTGCACCAAGGTAGCGAAACCGGTGGAGCCGGCAAAGCCGAGAAAATGCGCCATCACTTGTGTATCACACATCGGGCGCGGCAGCTGCTGAAAATAATGCTGGAATACGTCTAAATCTTCGCTACAAGCATGCAGAATTTTGACCAAATTAGTATCTgca                                                                                                                >  1:250129/1‑251 (MQ=255)                                                                                                                               tatTGCAGCTGCGTATCCGATAACGGGCGTGCCAGCCAATCGGTACGGGAGGCGCCTTTGTCGATTTCAATTTGGAAATAATGTTGCACCAAGGTAGCGAAACCGGTGGAGCCGGCAAAGCCGAGAAAATGCGCCATCACTTGTGTATCACACATCGGGCGCGGCAGCTGCTGAAAATAATGCTGGAATACGTCTAAATCTTCGCTACAAGCATGCAGAATTTTGACCAAATTAGTATCTGCAAGCAAttt                                                                                                        >  1:562150/1‑251 (MQ=255)                                                                                                                                 ttGCAGCTGCGTATCCGATAACGGGCGTGCCAGCCAATCGGTACGGGAGGCGCCTTTGTCGATTTCAATTTGGAAATAATGTTGCACCAAGGTAGCGAAACCGGTGGAGCCGGCAAAGCCGAGAAAATGCGCCATCACTTGTGTATCACACATCGGGCGCGGCAGCTGCTGAAAATAATGCTGGAATACGTCTAAATc                                                                                                                                                           <  1:480330/198‑1 (MQ=255)                                                                                                                                  tGCAGCTGCGTATCCGATAACGGGCGTGCCAGCCAATCGGTACGGGAGGCGCCTTTGTCGATTTCAATTTGGAAATAATGTTGCACCAAGGTAGCGAAACCGGTGGAGCCGGCAAAGCCGAGAAAATGCGCCATCACTTGTGTATCACACATCGGGCGCGGCAGCTGCTGAAAATAATGCTGGAATACGTCTAAATCTTCGCTACAAGCATGCAGAATTTTGACCAAATTAGTATCTGCAAGCAATTTAAt                                                                                                     <  1:289684/251‑1 (MQ=255)                                                                                                                                    cAGCTGCGTATCCGATAACGGGCGTGCCAGCCAATCGGTACGGGAGGCGCCTTTGTCGATTTCAATTTGGAAATAATGTTGCACCAAGGTAGCGAAACCGGTGGAGCCGGCAAAGCCGAGAAAATGCGCCATCACTTGTGTATCACACATCGGGCGCGGCAGCTGCTGAAAATAATGCTGGAATACGTCTAAATCTTCGCTACAAGCATGCAGAATTTTGACCAAATTAGTATCTGCAAGCAATTTAATg                                                                                                    <  1:280251/250‑1 (MQ=255)                                                                                                                                    cAGCTGCGTATCCGATAACGGGCGTGCCAGCCAATCGGTACGGGAGGCGCCTTTGTCGATTTCAATTTGGAAATAATGTTGCACCAAGGTAGCGAAACCGGTGGAGCCGGCAAAGCCGAGAAAATGCGCCATCACTTGTGTATCACACATCGGGCGCGGCAGCTGCTGAAAATAATGCTGGAATACGTCTAAATCTTCGCTACAAGCATGCAGAATTTTGACCAAATTAGTATCTGCAAGCAATTTAATg                                                                                                    <  1:808924/250‑1 (MQ=255)                                                                                                                                        tGCGTATCCGATAACGGGCGTGCCAGCCAATCGGTACGGGAGGCGCCTTTGTCGATTTCAATTTGGAAATAATGTTGCACCAAGGTAGCGAAACCGGTGGAGCCGGCAAAGCCGAGAAAATGCGCCATCACTTGTGTATCACACATCGGGCGCGGCAGCTGCTGAAAATAATGCTGGAATACGTCTAAATCTTCGCTACAAGCATGCAGAATTTTGACCAAATTAGTATCTGCAAGCAATTTAATGAACgg                                                                                               >  1:772453/1‑251 (MQ=255)                                                                                                                                           gTATCCGATAACGGGCGTGCCAGCCAATCGGTACGGGAGGCGCCTTTGTCGATTTCAATTTGGAAATAATGTTGCACCAAGGTAGCGAAACCGGTGGAGCCGGCAAAGCCGAGAAAATGCGCCATCACTTGTGTATCACACATCGGGCGCGGCAGCTGCTGAAAATAATGCTGGAATACGTCTAAATCTTCGCTACAAGCATGCAGAATTTTGACCAAATTAGTATCTGCAAGCAATTTAATGAACGGCGa                                                                                            >  1:618216/1‑251 (MQ=255)                                                                                                                                                 gATAACGGGCGTGCCAGCCAATCGGTACGGGAGGCGCCTTTGTCGATTTCAATTTGGAAATAATGTTGCACCAAGGTAGCGAAACCGGTGGAGCCGGCAAAGCCGAGAAAATGCGCCATCACTTGTGTAt                                                                                                                                                                                                               >  1:740199/1‑130 (MQ=255)                                                                                                                                                 gATAACGGGCGTGCCAGCCAATCGGTACGGGAGGCGCCTTTGTCGATTTCAATTTGGAAATAATGTTGCACCAAGGTAGCGAAACCGGTGGAGCCGGCAAAGCCGAGAAAATGCGCCATCACTTGTGTATCACACATCGGGCGCGGCAGCTGCTGAAAATAATGCTGGAATACGTCTAAATCTTCGCTACAAGCATGCAGAATTTTGACCAAATTAGTATCTGCAAGCAATTTAATGAACGGCGAAAAAt                                                                                       >  1:525269/1‑250 (MQ=255)                                                                                                                                                   tAACGGGCGTGCCAGCCAATCGGTACGGGAGGCGCCTTTGTCGATTTCAATTTGGAAATAATGTTGCACCAAGGTAGCGAAACCGGTGGAGCCGGCAAAGCCGAGAAAATGCGCCATCACTTGTGTATCACACATCGGGCGCGGCAGCTGCTGAAAATAATGCTGGAATACGTCTAAATCTTCGCTACAAGCATGCAGAATTTTGACCAAATTAGTATc                                                                                                                    >  1:285886/1‑219 (MQ=255)                                                                                                                                                       gggCGTGCCAGCCAATCGGTACGGGAGGCGCCTTTGTCGATTTCAATTTGGAAATAATGTTGCACCAAGGTAGCGAAACCGGTGGAGCCGGCAAAGCCGAGAAAATGCGCCATCACTTGTGTATCACACATCGGGCGCGGCAGCTGCTGAAAATAATGCTGGAATACGTCTAAATCTTCGCTACAAGCATGCAGAATTTTGACCAAATTAGTATCTGCAAGCAATTTAATGAACGGCGAAAAATCCTGAAt                                                                                >  1:467606/1‑251 (MQ=255)                                                                                                                                                                agccaATCGGTACGGGAGGCGCCTTTGTCGATTTCAATTTGGAAATAATGTTGCACCAAGGTAGCGAAACCGGTGGAGCCGGCAAAGCCGAGAAAATGCGCCATCACTTGTGTATCACACATCGGGCGCGGCAGCTGCTGAAAATAATGCTGGAATACGTCTAAATCTTCGCTACAAGCATGCAGAATTTTGACCAAATTAGTATCTGCAAGCAATTTAATGAACGGCGAAAAATCCTGAATCGGTAAggg                                                                       >  1:477708/1‑251 (MQ=255)                                                                                                                                                                  ccaATCGGTACGGGAGGCGCCTTTGTCGATTTCAATTTGGAAATAATGTTGCACCAAGGTAGCGAAACCGGTGGAGCCGGCAAAGCCGAGAAAATGCGCCATCACTTGTGTATCACACATCGGGCGCGGCAGCTGCTGAAAATAATGCTGGAATACGTCTAAATCTTCGCTACAAGCATGCAGAATTTTGACCAAATTAGTATCTGCAAGCAATTTAATGAACGGCGAAAAATCCTGAATCGGTAAggg                                                                       <  1:379119/249‑1 (MQ=255)                                                                                                                                                                  ccaATCGGTACGGGAGGCGCCTTTGTCGATTTCAATTTGGAAATAATGTTGCACCAAGGTAGCGAAACCGGTGGAGCCGGCAAAGCCGAGAAAATGCGCCATCACTTGTGTATCACACATCGGGCGCGGCAGCTGCTGAAAATAATGCTGGAATACGTCTAAATCTTCGCTACAAGCATGCAGAATTTTGACCAAATTAGTATCTGCAAGCAATTTAATGAACGGCGAAAAATCCTGAATCGGTAAGGGAt                                                                     <  1:475655/251‑1 (MQ=255)                                                                                                                                                                    aaTCGGTACGGGAGGCGCCTTTGTCGATTTCAATTTGGAAATAATGTTGCACCAAGGTAGCGAAACCGGTGGAGCCGGCAAAGCCGAGAAAAt                                                                                                                                                                                                                                 >  1:80315/1‑93 (MQ=255)                                                                                                                                                                    aaTCGGTACGGGAGGCGCCTTTGTCGATTTCAATTTGGAAATAATGTTGCACCAAGGTAGCGAAACCGGTGGAGCCGGCAAAGCCGAGAAAATGCGCCATCACTTGTGTATCACACATCGGGCGCGGCAGCTGCTGAAAATAATGCTGGAATACGTCTAAATCTTCGCTACAAGCATGCAg                                                                                                                                         <  1:498256/181‑1 (MQ=255)                                                                                                                                                                    aaTCGGTACGGGAGGCGCCTTTGTCGATTTCAATTTGGAAATAATGTTGCACCAAGGTAGCGAAACCGGTGGAGCCGGCAAAGCCGAGAAAATGCGCCATCACTTGTGTATCACACATCGGGCGCGGCAGCTGCTGAAAATAATGCTGGAATACGTCTAAATCTTCGCTACAAGCATGCAGAATTTTGACCAAATTAGTATCTGCAAGCAATTTAATGAACGGCGAAAAATCCTGAATCGGTAAGGGATCg                                                                   >  1:43391/1‑251 (MQ=255)                                                                                                                                                                      tCGGTACGGGAGGCGCCTTTGTCGATTTCAATTTGGAAATAATGTTGCACCAAGGTAGCGAAACCGGTGGAGCCGGCAAAGCCGAGAAAATGCGCCATCACTTGTGTATCACACATCGGGCGCGGCAGCTGCTGAAAATAATGCTGGAATACGTCTAAATCTTCGCTACAAGCATGCAGAATTTTGACCAAATTAGTATCTGCAAGCAATTTAATGAACGGCGAAAAATCCTGAATCGGTAAGGGATCGAt                                                                 >  1:479443/1‑251 (MQ=255)                                                                                                                                                                      tCGGTACGGGAGGCGCCTTTGTCGATTTCAATTTGGAAATAATGTTGCACCAAGGTAGCGAAACCGGTGGAGCCGGCAAAGCCGAGAAAATGCGCCATCACTTGTGTATCACACATCGGGCGCGGCAGCTGCTGAAAATAATGCTGGAATACGTCTAAATCTTCGCTACAAGCATGCAGAATTTTGACCAAATTAGTATCTGCAAGCAATTTAATGAACGGCGAAAAATCCTGAATCGGTAAGGGATCGAt                                                                 <  1:693474/251‑1 (MQ=255)                                                                                                                                                                       cGGTACGGGAGGCGCCTTTGTCGATTTCAATTTGGAAATAATGTTGCACCAAGGTAGCGAAACCGGTGGAGCCGGCAAAGCCGAGAAAATGCGCCATCACTTGTGTATCACACATCGGGCGCGGCAGCTGCTGAAAATAATGCTGGAATACGTCTAAATc                                                                                                                                                           >  1:369996/1‑160 (MQ=255)                                                                                                                                                                        ggTACGGGAGGCGCCTTTGTCGATTTCAATTTGGAAATAATGTTGCACCAAGGTAGCGAAACCGGTGGAGCCGGCAAAGCCGAGAAAATGCGCCATCACTTGTGTATCACACATCGGGCGCGGCAGCTGCTGAAAATAATGCTgg                                                                                                                                                                         <  1:648654/145‑1 (MQ=255)                                                                                                                                                                         gTACGGGAGGCGCCTTTGTCGATTTCAATTTGGAAATAATGTTGCACCAAGGTAGCGAAACCGGTGGAGCCGGCAAAGCCGAGAAAATGCGCCATCACTTgtgt                                                                                                                                                                                                                 >  1:183347/1‑104 (MQ=255)                                                                                                                                                                         gTACGGGAGGCGCCTTTGTCGATTTCAATTTGGAAATAATGTTGCACCAAGGTAGCGAAACCGGTGGAGCCGGCAAAGCCGAGAAAATGCGCCATCACTTGTGTATCACACATCGGGCGCGGCAGCTGCTGaaa                                                                                                                                                                                   >  1:746233/1‑134 (MQ=255)                                                                                                                                                                         gTACGGGAGGCGCCTTTGTCGATTTCAATTTGGAAATAATGTTGCACCAAGGTAGCGAAACCGGTGGAGCCGGCAAAGCCGAGAAAATGCGCCATCACTTGTGTATCACACATCGGGCGCGGCAGCTGCTGAAAATAATGCTGGAATACGTCTAAATCTTCGCTACAAGCa                                                                                                                                              >  1:342445/1‑171 (MQ=255)                                                                                                                                                                         gTACGGGAGGCGCCTTTGTCGATTTCAATTTGGAAATAATGTTGCACCAAGGTAGCGAAACCGGTGGAGCCGGCAAAGCCGAGAAAATGCGCCATCACTTGTGTATCACACATCGGGCGCGGCAGCTGCTGAAAATAATGCTGGAATACGTCTAAATCTTCGCTACAAGCATGCAGAATTTTGACCAAATTAGTATCTGCAAGCAATTTAATGAACGGCGAAAAATCCTGAATCGGTAAGGGATCGATTaa                                                              >  1:439451/1‑251 (MQ=255)                                                                                                                                                                         gTACGGGAGGCGCCTTTGTCGATTTCAATTTGGAAATAATGTTGCACCAAGGTAGCGAAACCGGTGGAGCCGGCAAAGCCGAGAAAATGCGCCATCACTTGTGTATCACACATCGGGCGCGGCAGCTGCTGAAAATAATGCTGGAATACGTCTAAATCTTCGCTACAAGCATGCAGAATTTTGACCAAATTAGTATCTGCAAGCAATTTAATGAACGGCGAAAAATCCTGAATCGGTAAGGGATCGATTaa                                                              >  1:75632/1‑251 (MQ=255)                                                                                                                                                                         gTACGGGAGGCGCCTTTGTCGATTTCAATTTGGAAATAATGTTGCACCAAGGTAGCGAAACCGGTGGAGCCGGCAAAGCCGAGAAAATGCGCCATCACTTGTGTATCACACATCGGGCGCGGCAGCTGCTGAAAATAATGCTGGAATACGTCTAAATCTTCGCTACAAGCATGCAGAATTTTGACCAAATTAGTATCTGCAAGCAATTTAATGAACGGCGAAAAATCCTGAATCGGTAAGGGATCGATTaa                                                              >  1:49006/1‑251 (MQ=255)                                                                                                                                                                                     ccTTTGTCGATTTCAATTTGGAAATAATGTTGCACCAAGGTAGCGAAACCGGTGGAGCCGGCAAAGCCGAGAAAATGCGCCATCACTTGTGTATCACACATCGGGCGCGGCAGCTGCTGAAAATAATGCTGGAATACGTCTAAATCTTCGCTACAAGCATGCAGAATTTTGACCAAATTAGTATCTGCAAGCAATTTAATGAACGGCGAAAAATCCTGAATCGGTAAGGGATCGATTAACGCCACCCGAt                                                   >  1:688991/1‑250 (MQ=255)                                                                                                                                                                                     ccTTTGTCGATTTCAATTTGGAAATAATGTTGCACCAAGGTAGCGAAACCGGTGGAGCCGGCAAAGCCGAGAAAATGCGCCATCACTTGTGTATCACACATCGGGCGCGGCAGCTGCTGAAAATAATGCTGGAATACGTCTAAATCTTCGCTACAAGCATGCAGAATTTTGACCAAATTAGTATCTGCAAGCAATTTAATGAACGGCGAAAAATCCTGAATCGGTAAGGGATCGATTAACGCCACCCGAt                                                   >  1:245052/1‑250 (MQ=255)                                                                                                                                                                                                    aTTTGGAAATAATGTTGCACCAAGGTAGCGAAACCGGTGGAGCCGGCAAAGCCGAGAAAATGCGCCATCACTTGTGTATCACACATCGGGCGCGGCAGCTGCTGAAAATAATGCTGGAATACGTCTAAATCTTCGCTACAAGCATGCAGAATTTTGACCAAATTAGTATCTGCAAGCAATTTAATGAACGGCGAAAAATCCTGAATCGGTAAGGGATCGATTAACGCCACCCGATCGCCGAAATACAt                                      >  1:635467/1‑248 (MQ=255)                                                                                                                                                                                                       tGGAAATAATGTTGCACCAAGGTAGCGAAACCGGTGGAGCCGGCAAAGCCGAGAAAATGCGCCATCACTTGTGTATCACACATCGGGCGCGGCAGCTGCTGAAAATAATGCTGGAATACGTCTAAATCTTCGCTACAAGCATGCAGAATTTTGACCAAATTAGTATCTGCAAGCAATTTAATGAACGGCGAAAAATCCTGAATCGGTAAGGGATCGATTAACGCCACCCGATCGCCGAAATACATCTGAAt                                <  1:167947/251‑1 (MQ=255)                                                                                                                                                                                                         gAAATAATGTTGCACCAAGGTAGCGAAACCGGTGGAGCCGGCAAAGCCGAGAAAATGCGCCATCACTTGTGTATCACACATCGGGCGCGGCAGCTGCTGAAAATAATGCTGGAATACGTCTAAATCTTCGCTACAAGCATGCAGAATTTTGACCAAAtt                                                                                                                          >  1:840890/1‑159 (MQ=255)                                                                                                                                                                                                            ataatGTTGCACCAAGGTAGCGAAACCGGTGGAGCCGGCAAAGCCGAGAAAATGCGCCATCACTTGTGTATCACACATCGGGCGCGGCAGCTGCTGAAAATAATGCTGGAATACGTCTAAATCTTCGCTACAAGCATGCAGAATTTTGACCAAATTAGTATCTGCAAGCAATTTAATGAACGGCGAAAAATCCTGAATCGGTAAGGGATCGATTAACGCCACCCGATCGCCGAAATACATCTGAATTAAAc                           <  1:857510/251‑1 (MQ=255)                                                                                                                                                                                                               atGTTGCACCAAGGTAGCGAAACCGGTGGAGCCGGCAAAGCCGAGAAAATGCGCCATCACTTGTGTAt                                                                                                                                                                                                               <  1:84361/68‑1 (MQ=255)                                                                                                                                                                                                               atGTTGCACCAAGGTAGCGAAACCGGTGGAGCCGGCAAAGCCGAGAAAATGCGCCATCACTTGTGTATCACACATCGGGCGCGGCAGCTGCTGAAAATAATGCTGGAATACGTCTAAATCTTCGCTACAAGCATGCAGAATTTTGACCAAATTAGTATCTGCAAGCAATTTAATGAACGGCGAAAAATCCTGAATCGGTAAGGGATCGATTAACGCCACCCGATCGCCGAAAt                                          <  1:183755/233‑1 (MQ=255)                                                                                                                                                                                                               atGTTGCACCAAGGTAGCGAAACCGGTGGAGCCGGCAAAGCCGAGAAAATGCGCCATCACTTGTGTATCACACATCGGGCGCGGCAGCTGCTGAAAATAATGCTGGAATACGTCTAAATCTTCGCTACAAGCATGCAGAATTTTGACCAAATTAGTATCTGCAAGCAATTTAATGAACGGCGAAAAATCCTGAATCGGTAAGGGATCGATTAACGCCACCCGATCGCCGAAATACATCTGAATTAAACCGa                        >  1:534217/1‑251 (MQ=255)                                                                                                                                                                                                                     cACCAAGGTAGCGAAACCGGTGGAGCCGGCAAAGCCGAGAAAATGCGCCATCACTTGTGTATCACACATCGGGCGCGGCAGCTGCTGAAAATAATGCTGGAATACGTCTAAATCTTCGCTACAAGCATGCAGAATTTTGACCAAATTAGTATCTGCAAGCAATTTAATGAACGGCGAAAAATCCTGAATCGGTAAGGGATCGATTAACGCCACCCGATCGCCGAAATACATCTGAATTAAACCGAGTTTCg                  >  1:9878/1‑251 (MQ=255)                                                                                                                                                                                                                         aaGGTAGCGAAACCGGTGGAGCCGGCAAAGCCGAGAAAATGCGCCATCACTTGTGTATCACACATCGGGCGCGGCAGCTGCTGAAAATAATGCTGGAATACGTCTAAATCTTCGCTACAAGCATGCAGAATTTTGACCAAATTAGTATCTGCAAGCAATTTAATGAACGGCGAAAAATCCTGAATCGGTAAGGGAt                                                                     >  1:701398/1‑196 (MQ=255)                                                                                                                                                                                                                                     ccGGTGGAGCCGGCAAAGCCGAGAAAATGCGCCATCACTTGTGTATCACACATCGGGCGCGGCAGCTGCTGAAAATAATGCTGGAATACGTCTAAATCTTCGCTACAAGCATGCAGAATTTTGACCAAATTAGTATCTGCAAGCAATTTAATGAACGGCGAAAAATCCTGAATCGGTAAGGGAt                                                                     >  1:677103/1‑184 (MQ=255)                                                                                                                                                                                                                                     ccGGTGGAGCCGGCAAAGCCGAGAAAATGCGCCATCACTTGTGTATCACACATCGGGCGCGGCAGCTGCTGAAAATAATGCTGGAATACGTCTAAATCTTCGCTACAAGCATGCAGAATTTTGACCAAATTAGTATCTGCAAGCAATTTAATGAACGGCGAAAAATCCTGAATCGGTAAGGGATCGATTAACGCCACCCGATCGCCGAAATACATCTGAATTAAACCGAGTTTCGGATACAGGGTTCGTAc  <  1:394278/251‑1 (MQ=255)                                                                                                                                                                                                                                             gCCGGCAAAGCCGAGAAAATGCGCCATCACTTGTGTATCACACATCGGGCGCGGCAGCTGCTGAAAATAATGCTGGAATACGTCTAAATCTTCGCTACAAGCATGCAGAATTTTGACCAAATTAGTATCTGCAAGCAATTTAATGa                                                                                                   >  1:890534/1‑146 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                             GTCTGCGCCCGCTTGTTCAATAAAAATTCACATTCATTTTCCACCACACTTTGCCACTCGGTTTGCGTAAGCTGTTGTTGCATTTGTTCATATAACGGCAACAAATACCATACATCCGCCGCCGCATATTGCAGCTGCGTATCCGATAACGGGCGTGCCAGCCAATCGGTACGGGAGGCGCCTTTGTCGATTTCAATTTGGAAATAATGTTGCACCAAGGTAGCGAAACCGGTGGAGCCGGCGAAGCCGAGAAAATGCGCCATCACTTGTGTATCACACATCGGGCGCGGCAGCTGCTGAAAATAATGCTGGAATACGTCTAAATCTTCGCTACAAGCATGCAGAATTTTGACCAAATTAGTATCTGCAAGCAATTTAATGAACGGCGAAAAATCCTGAATCGGTAAGGGATCGATTAACGCCACCCGATCGCCGAAATACATCTGAATTAAACCGAGTTTCGGATACAGGGTTCGTAC  >  1/1846933‑1847411

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑