Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,815,131	G→A	intergenic (+55704/‑19225)	unknown → / → unknown	tRNA‑Asn‑GTT/tRNA‑Leu‑CAA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,815,131	0	G	A	100.0%	209.5 / NA	60	intergenic (+55704/‑19225)	unknown/unknown	tRNA‑Asn‑GTT/tRNA‑Leu‑CAA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (31/29);  total (31/29)

CCTCTCCTGCCATTCGTATGTTGGTGAAAGCCACCGCGCAAAAGCTGGGAGAAGATAAAGGCAATAGCCGTTATTTGCAACGTTTATTTCCCGATGGTCCGGCAAAACAGGCAACCAAGCTGGCGGGTCTCCCCAAACCGGTAAAATGTCTATAATTTTTTTGAGAATCATTTGCATTTAGAAATAGAAAGAATATAATGTCCGCGCAGCTTATGTTGTTATTTTATAGTTAAAAAGCCAAGGAGGAAAAATATGCGTTTATTAGCCACATTAGTCAGCGTACTTGCGTTAAGCTTAAGCAGTAGTCTTGCTTATGCGAAATTTAAAGTTGTCACTACGTTCACTATTATTCAAGATATGGCACAAAATGTCGCCGGTGATGCGGCAACCGTAGAGTCCATCACCAAGCCCGGAGCAGAAATCCATGATTACGAGCCAACCCCGAAAGATATTGTCAAGGCACAATCTGCCGATTTAGTGTTATGGAA  >  1/1814886‑1815373                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                   ccTCTCCTGCCATTCGTATGTTGGTGAAAGCCACCGCGCAAAAGCTGGGAGAAGATAAAGGCAATAGCCGTTATTTGCAACGTTTATTTCCCGATGGTCCGGCAAAACAGGCAACCAAGCTGGCGGGTCTCCCCAAACCGGTAAAATGTCTATAATTTTTTTGAGAATCATTTGCATTTAGAAATAGAAAGAATATAATGTCCGCGCAGCTTATGTTGTTATTTTATAGTTAAAAAGCCAAGGAGaaaaaa                                                                                                                                                                                                                                               <  1:537832/251‑1 (MQ=255)         gCCATTCGTATGTTGGTGAAAGCCACCGCGCAAAAGCTGGGAGAAGATAAAGGCAATAGCCGTTATTTGCAACGTTTATTTCCCGATGGTCCGGCAAAACAGGCAACCAAGCTGGCGGGTCTCCCCAAACCGGTAAAATGTCTATAATTTTTTTGAGAATCATTTGCATTTAGAAATAGAAAGAATATAATGTCCGCGCAGCTTATGTTGTTATTTTATAGTTAAAAAGCCAAGGAGaaaaaa                                                                                                                                                                                                                                               >  1:381526/1‑243 (MQ=255)           cATTCGTATGTTGGTGAAAGCCACCGCGCAAAAGCTGGGAGAAGATAAAGGCAATAGCCGTTATTTGCAACGTTTATTTCCCGATGGTCCGGCAAAACAGGCAACCAAGCTGGCGGGTCTCCCCAAACCGGTAAAATGTCTATAATTTTTTTGAGAATCATTTGCATTTAGAAATAGAAAGAATATAATGTCCGCGCAGCTTATGTTGTTATTTTATAGTTAAAAAGCCAAGGAGAAAAAATATGCGTtta                                                                                                                                                                                                                                     <  1:850156/251‑1 (MQ=255)            aTTCGTATGTTGGTGAAAGCCACCGCGCAAAAGCTGGGAGAAGATAAAGGCAATAGCCGTTATTTGCAACGTTTATTTCCCGATGGTCCGGCAAAACAGGCAACCAAGCTGGCGGGTCTCCCCAAACCGGTAAAATGTCTATAATTTTTTTGAGAATCATTTGCATTTAGAAATAGAAAGAATATAATGTCCGCGCAGCTTATGTTGTTATTTTATAGTTAAAAAGCCAAGGAGAAAAAATATGCGTttat                                                                                                                                                                                                                                    >  1:64543/1‑251 (MQ=255)            aTTCGTATGTTGGTGAAAGCCACCGCGCAAAAGCTGGGAGAAGATAAAGGCAATAGCCGTTATTTGCAACGTTTATTTCCCGATGGTCCGGCAAAACAGGCAACCAAGCTGGCGGGTCTCCCCAAACCGGTAAAATGTCTATAATTTTTTTGAGAATCATTTGCATTTAGAAATAGAAAGAATATAATGTCCGCGCAGCTTATGTTGTTATTTTATAGTTAAAAAGCCAAGGAGAAAAAATATGCGTttat                                                                                                                                                                                                                                    >  1:419654/1‑251 (MQ=255)              tCGTATGTTGGTGAAAGCCACCGCGCAAAAGCTGGGAGAAGATAAAGGCAATAGCCGTTATTTGCAACGTTTATTTCCCGATGGTCCGGCAAAACAGGCAACCAAGCTGGCGGGTCTCCCCAAACCGGTAAAATGTCTATAATTTTTTTGAGAATCATTTGCATTTAGAAATAGAAAGAATATAATGTCCGCGCAGCTTATGTTGTTATTTTATAGTTAAAAAGCCAAGGAGAAAAAATATGCGTttatta                                                                                                                                                                                                                                  >  1:172854/1‑251 (MQ=255)                  aTGTTGGTGAAAGCCACCGCGCAAAAGCTGGGAGAAGATAAAGGCAATAGCCGTTATTTGCAACGTTTATTTCCCGATGGTCCGGCAAAACAGGCAACCAAGCTGGCGGGTCTCCCCAAACCGGTAAAATGTCTATAATTTTTTTGAGAATCATTTGCATTTAGAAATAGAAAGAATATAATGTCCGCGCAGCTTATGTTGTTATTTTATAGTTAAAAAGCCAAGGAGAAAAAATATGCGTTTATTAGCca                                                                                                                                                                                                                              >  1:747392/1‑251 (MQ=255)                               ccACCGCGCAAAAGCTGGGAGAAGATAAAGGCAATAGCCGTTATTTGCAACGTTTATTTCCCGATGGTCCGGCAAAACAGGCAACCAAGCTGGCGGGTCTCCCCAAACCGGTAAAATGTCTATAATTTTTTTGAGAATCATTTGCATTTAGAAATAGAAAGAATATAATGTCCGCGCAGCTTATGTTGTTATTTTATAGTTAAAAAGCCAAGGAGAAAAAATATGCGTTTATTAGCCACATTAGTCAGCGt                                                                                                                                                                                                                 >  1:266509/1‑251 (MQ=255)                                             cTGGGAGAAGATAAAGGCAATAGCCGTTATTTGCAACGTTTATTTCCCGATGGTCCGGCAAAACAGGCAACCAAGCTGGCGGGTCTCCCCAAACCGGTAAAATGTCTATAATTTTTTTGAGAATCATTTGCATTTAGAAATAGAAAGAATATAATGTCCGCGCAGCTTATGTTGTTATTTTATAGTTAAAAAGCCAAGGAGAAAAAATATGCGTTTATTAGCCACATTAGTCAGCGTACTTGCGttaagct                                                                                                                                                                                                   <  1:263752/251‑1 (MQ=255)                                             cTGGGAGAAGATAAAGGCAATAGCCGTTATTTGCAACGTTTATTTCCCGATGGTCCGGCAAAACAGGCAACCAAGCTGGCGGGTCTCCCCAAACCGGTAAAATGTCTATAATTTTTTTGAGAATCATTTGCATTTAGAAATAGAAAGAATATAATGTCCGCGCAGCTTATGTTGTTATTTTATAGTTAAAAAGCCAAGGAGAAAAAATATGCGTTTATTAGCCACATTAGTCAGCGTACTTGCGttaagct                                                                                                                                                                                                   <  1:8668/251‑1 (MQ=255)                                             cTGGGAGAAGATAAAGGCAATAGCCGTTATTTGCAACGTTTATTTCCCGATGGTCCGGCAAAACAGGCAACCAAGCTGGCGGGTCTCCCCAAACCGGTAAAATGTCTATAATTTTTTTGAGAATCATTTGCATTTAGAAATAGAAAGAATATAATGTCCGCGCAGCTTATGTTGTTATTTTATAGTTAAAAAGCCAAGGAGAAAAAATATGCGTTTATTAGCCACATTAGTCAGCGTACTTGCGttaagc                                                                                                                                                                                                    <  1:849960/250‑1 (MQ=255)                                             cTGGGAGAAGATAAAGGCAATAGCCGTTATTTGCAACGTTTATTTCCCGATGGTCCGGCAAAACAGGCAACCAAGCTGGCGGGTCTCCCCAAACCGGTAAAATGTCTATAATTTTTTTGAGAATCATTTGCATTTAGAAATAGAAAGAATATAATGTCCGCGCAGCTTATGTTGTTATTTTATAGTTAAAAAGCCAAGGAGAAAAAATATGCGTTTATTAGCCACATTAGTCAGCGTACTTGCGttaagc                                                                                                                                                                                                    <  1:582697/250‑1 (MQ=255)                                                     agaTAAAGGCAATAGCCGTTATTTGCAACGTTTATTTCCCGATGGTCCGGCAAAACAGGCAACCAAGCTGGCGGGTCTCCCCAAACCGGTAAAATGTCTATAATTTTTTTGAGAATCATTTGCATTTAGAAATAGAAAGAATATAATGTCCGCGCAGCTTATGTTGTTATTTTATAGTTAAAAAGCCAAGGAGAAAAAATATGCGTTTATTAGCCACATTAGTCAGCGt                                                                                                                                                                                                                 <  1:658978/229‑1 (MQ=255)                                                     agaTAAAGGCAATAGCCGTTATTTGCAACGTTTATTTCCCGATGGTCCGGCAAAACAGGCAACCAAGCTGGCGGGTCTCCCCAAACCGGTAAAATGTCTATAATTTTTTTGAGAATCATTTGCATTTAGAAATAGAAAGAATATAATGTCCGCGCAGCTTATGTTGTTATTTTATAGTTAAAAAGCCAAGGAGAAAAAATATGCGTTTATTAGCCACATTAGTCAGCGTACTTGCGTTAAGCTTAAGCagt                                                                                                                                                                                           >  1:366663/1‑251 (MQ=255)                                                      gaTAAAGGCAATAGCCGTTATTTGCAACGTTTATTTCCCGATGGTCCGGCAAAACAGGCAACCAAGCTGGCGGGTCTCCCCAAACCGGTAAAATGTCTATAATTTTTTTGAGAATCATTTGCATTTAGAAATAGAAAGAATATAATGTCCGCGCAGCTTATGTTGTTATTTTATAGTTAAAAAGCCAAGGAGAAAAAATATGCGTTTATTAGCcac                                                                                                                                                                                                                             <  1:156061/216‑1 (MQ=255)                                                       aTAAAGGCAATAGCCGTTATTTGCAACGTTTATTTCCCGATGGTCCGGCAAAACAGGCAACCAAGCTGGCGGGTCTCCCCAAACCGGTAAAATGTCTATAATTTTTTTGAGAATCATTTGCATTTAGAAATAGAAAGAATATAATGTCCGCGCAGCTTATGTTGTTATTTTATAGTTAAAAAGCCAAGGAGAAAAAATATGCGTTTATTAGCCACATTAGTCAGCGTACTTGCGTTAAGCTTAAGCagtag                                                                                                                                                                                         <  1:206439/251‑1 (MQ=255)                                                       aTAAAGGCAATAGCCGTTATTTGCAACGTTTATTTCCCGATGGTCCGGCAAAACAGGCAACCAAGCTGGCGGGTCTCCCCAAACCGGTAAAATGTCTATAATTTTTTTGAGAATCATTTGCATTTAGAAATAGAAAGAATATAATGTCCGCGCAGCTTATGTTGTTATTTTATAGTTAAAAAGCCAAGGAGAAAAAATATGCGTTTATTAGCCACATTAGTCAGCGTACTTGCGTTAAGCTTAAGCagtag                                                                                                                                                                                         <  1:327095/251‑1 (MQ=255)                                                            ggCAATAGCCGTTATTTGCAACGTTTATTTCCCGATGGTCCGGCAAAACAGGCAACCAAGCTGGCGGGTCTCCCCAAACCGGTAAAATGTCTATAATTTTTTTGAGAATCATTTGCATTTAGAAATAGAAAGAATATAATGTCCGCGCAGCTTATGTTGTTATTTTATAGTTAAAAAGCCAAGGAGAAAAAATATGCGTTTATTAGCCACATTAGTc                                                                                                                                                                                                                      <  1:533619/217‑1 (MQ=255)                                                            ggCAATAGCCGTTATTTGCAACGTTTATTTCCCGATGGTCCGGCAAAACAGGCAACCAAGCTGGCGGGTCTCCCCAAACCGGTAAAATGTCTATAATTTTTTTGAGAATCATTTGCATTTAGAAATAGAAAGAATATAATGTCCGCGCAGCTTATGTTGTTATTTTATAGTTAAAAAGCCAAGGAGAAAAAATATGCGTTTATTAGCCACATTAGTCAGCGTACTTGCGTTAAGCTTAAGCAGTAGTCTTg                                                                                                                                                                                    >  1:666792/1‑251 (MQ=255)                                                               aaTAGCCGTTATTTGCAACGTTTATTTCCCGATGGTCCGGCAAAACAGGCAACCAAGCTGGCGGGTCTCCCCAAACCGGTAAAATGTCTATAATTTTTTTGAGAATCATTTGCATTTAGAAATAGAAAGAATATAATGTCCGCGCAGCTTATGTTGTTATTTTATAGTTAAAAAGCCAAGGAGAAAAAATATGCGTTTATTAGCCACATTAGTCAGCGTACTTGCGTTAAGCTTAAGCAGTAGTCTTGCtt                                                                                                                                                                                 <  1:705330/251‑1 (MQ=255)                                                                    ccGTTATTTGCAACGTTTATTTCCCGATGGTCCGGCAAAACAGGCAACCAAGCTGGCGGGTCTCCCCAAACCGGTAAAATGTCTATAATTTTTTTGAGAATCATTTGCATTTAGAAATAGAAAGAATATAATGTCCGCGCAGCTTATGTTGTTATTTTATAGTTAAAAAGCCAAGGAGAAAAAATATGCGTTTATTAGCCACATTAGTCAGCGTACTTGCGTTAAGCTTAAGCAGTAGTCTTGCTTATGCg                                                                                                                                                                            >  1:606845/1‑251 (MQ=255)                                                                        tATTTGCAACGTTTATTTCCCGATGGTCCGGCAAAACAGGCAACCAAGCTGGCGGGTCTCCCCAAACCGGTAAAATGTCTATAATTTTTTTGAGAATCATTTGCATTTAGAAATAGAAAGAATATAATGTCCGCGCAGCTTATGTTGTTATTTTATAGTTAAAAAGCCAAGGAGAAAAAATATGCGTTTATTAGCCACATTAGTCAGCGt                                                                                                                                                                                                                 <  1:57236/210‑1 (MQ=255)                                                                                         tCCCGATGGTCCGGCAAAACAGGCAACCAAGCTGGCGGGTCTCCCCAAACCGGTAAAATGTCTATAATTTTTTTGAGAATCATTTGCATTTAGAAATAGAAAGAATATAATGTCCGCGCAGCTTATGTTGTTATTTTATAGTTAAAAAGCCAAGGAGAAAAAATATGCGTTTATTAGCCACATTAGTCAGCGTACTTGCGTTAAGCTTAAGCAGTAGTCTTGCTTATGCGAAATTTAAAGTTGTCACTACg                                                                                                                                                       <  1:822048/251‑1 (MQ=255)                                                                                                       cAAAACAGGCAACCAAGCTGGCGGGTCTCCCCAAACCGGTAAAATGTCTATAATTTTTTTGAGAATCATTTGCATTTAGAAATAGAAAGAATATAATGTCCGCGCAGCTTATGTTGTTATTTTATAGTTAAAAAGCCAAGGAGAAAAAATATGCGTTTATTAGCCACATTAGTCAGCGTACTTGCGTTAAGCTTAAGCAGTAGTCTTGCTTATGCGAAATTTAAAGTTGTCACTACGTTCACTATTATTc                                                                                                                                          <  1:617294/250‑1 (MQ=255)                                                                                                            cAGGCAACCAAGCTGGCGGGTCTCCCCAAACCGGTAAAATGTCTATAATTTTTTTGAGAATCATTTGCATTTAGAAATAGAAAGAATATAATGTCCGCGCAGCTTATGTTGTTATTTTATAGTTAAAAAGCCAAGGAGAAAAAATATGCGTTTATTAGCCACATTAGTCAGCGTACTTGCGTTAAGCTTAAGCAGTAGTCTTGCTTATGCGAAATTTAAAg                                                                                                                                                                  <  1:543361/221‑1 (MQ=255)                                                                                                                                  tcCCCAAACCGGTAAAATGTCTATAATTTTTTTGAGAATCATTTGCATTTAGAAATAGAAAGAATATAATGTCCGCGCAGCTTATGTTGTTATTTTATAGTTAAAAAGCCAAGGAGAAAAAATATGCGTTTATTAGCCACATTAGTCAGCGTACTTGCGTTAAGCTTAAGCAGTAGTCTTGCTTATGCGAAATTTAAAGTTGTCACTACGTTCACtatta                                                                                                                                             >  1:159691/1‑220 (MQ=255)                                                                                                                                   ccccAAACCGGTAAAATGTCTATAATTTTTTTGAGAATCATTTGCATTTAGAAATAGAAAGAATATAATGTCCGCGCAGCTTATGTTGTTATTTTATAGTTAAAAAGCCAAGGAGAAAAAATATGCGTTTATTAGCCACATTAGTCAGCGTACTTGCGTTAAGCTTAAGCAGTAGTCTTGCTTATGCGAAATTTAAAGTTGTCACTACGTTCACTATTATTc                                                                                                                                          >  1:184892/1‑222 (MQ=255)                                                                                                                                   ccccAAACCGGTAAAATGTCTATAATTTTTTTGAGAATCATTTGCATTTAGAAATAGAAAGAATATAATGTCCGCGCAGCTTATGTTGTTATTTTATAGTTAAAAAGCCAAGGAGAAAAAATATGCGTTTATTAGCCACATTAGTCAGCGTACTTGCGTTAAGCTTAAGCAGTAGTCTTGCTTATGCGAAATTTAAAGTTGTCACTACGTTCACTATTATTCAAGATATGGCACAAAATGTCGCCGGTGAt                                                                                                             >  1:394094/1‑251 (MQ=255)                                                                                                                                   ccccAAACCGGTAAAATGTCTATAATTTTTTTGAGAATCATTTGCATTTAGAAATAGAAAGAATATAATGTCCGCGCAGCTTATGTTGTTATTTTATAGTTAAAAAGCCAAGGAGAAAAAATATGCGTTTATTAGCCACATTAGTCAGCGTACTTGCGTTAAGCTTAAGCAGTAGTCTTGCTTATGCGAAATTTAAAGTTGTCACTACGTTCACTATTATTCAAGATATGGCACAAAATGTCGCCGGTGAt                                                                                                             >  1:411878/1‑251 (MQ=255)                                                                                                                                          ccGGTAAAATGTCTATAATTTTTTTGAGAATCATTTGCATTTAGAAATAGAAAGAATATAATGTCCGCGCAGCTTATGTTGTTATTTTATAGTTAAAAAGCCAAGGAGAAAAAATATGCGTTTATTAGCCACATTAGTCAGCGTACTTg                                                                                                                                                                                                            <  1:268376/149‑1 (MQ=255)                                                                                                                                            ggTAAAATGTCTATAATTTTTTTGAGAATCATTTGCATTTAGAAATAGAAAGAATATAATGTCCGCGCAGCTTATGTTGTTATTTTATAGTTAAAAAGCCAAGGAGAAAAAATATGCGTTTATTAGCCACATTAGTCAGCGTACTTGCGTTAAGCTTAAGCAGTAGTCTTGCTTATGCGAAATTTAAAGTTGTCACTACGTTCACt                                                                                                                                                 >  1:516975/1‑206 (MQ=255)                                                                                                                                                    gTCTATAATTTTTTTGAGAATCATTTGCATTTAGAAATAGAAAGAATATAATGTCCGCGCAGCTTATGTTGTTATTTTATAGTTAAAAAGCCAAGGAGAAAAAATATGCGTTTATTAGcc                                                                                                                                                                                                                               <  1:462425/120‑1 (MQ=255)                                                                                                                                                    gTCTATAATTTTTTTGAGAATCATTTGCATTTAGAAATAGAAAGAATATAATGTCCGCGCAGCTTATGTTGTTATTTTATAGTTAAAAAGCCAAGGAGAAAAAATATGCGTTTATTAGCCACATTAGTCAGCGTACTTGCGTTAAGCTTAAGCAGTAGTCTTGCTTATGCGAAATTTAAAGTTGTCACTACGTTCACTATTATTCAAGATATGGCACAAAATGTCGCCGGTGATGCGGCAACCGTAGAGt                                                                                             >  1:529695/1‑250 (MQ=255)                                                                                                                                                                        tCATTTGCATTTAGAAATAGAAAGAATATAATGTCCGCGCAGCTTATGTTGTTATTTTATAGTTAAAAAGCCAAGGAGAAAAAATATGCGTTTATTAGCCACATTAGTc                                                                                                                                                                                                                      <  1:663624/109‑1 (MQ=255)                                                                                                                                                                         cATTTGCATTTAGAAATAGAAAGAATATAATGTCCGCGCAGCTTATGTTGTTATTTTATAGTTAAAAAGCCAAGGAGAAAAAATATGCGTTTATTAGCCACATTAGTCAGCGTACTTGCGTTAAGCTTAAGCAGTAGTCTTGCt                                                                                                                                                                                  >  1:495708/1‑144 (MQ=255)                                                                                                                                                                         cATTTGCATTTAGAAATAGAAAGAATATAATGTCCGCGCAGCTTATGTTGTTATTTTATAGTTAAAAAGCCAAGGAGAAAAAATATGCGTTTATTAGCCACATTAGTCAGCGTACTTGCGTTAAGCTTAAGCAGTAGTCTTGCTTATGCGAAATTTAAAGTTGTCACTACGTTCACTATTATTCAAGATATGGCACAAAATGTCGCCGGTGATGCGGCAACCGTAGAGTCCATCACCAAGCCCGGAGCAGa                                                                       >  1:486568/1‑251 (MQ=255)                                                                                                                                                                         cATTTGCATTTAGAAATAGAAAGAATATAATGTCCGCGCAGCTTATGTTGTTATTTTATAGTTAAAAAGCCAAGGAGAAAAAATATGCGTTTATTAGCCACATTAGTCAGCGTACTTGCGTTAAGCTTAAGCAGTAGTCTTGCTTATGCGAAATTTAAAGTTGTCACTACGTTCACTATTATTCAAGATATGGCACAAAATGTCGCCGGTGATGCGGCAACCGTAGAGTCCATCACCAAGCCCGGAGCAGa                                                                       >  1:128523/1‑251 (MQ=255)                                                                                                                                                                                      aaatagaaaGAATATAATGTCCGCGCAGCTTATGTTGTTATTTTATAGTTAAAAAGCCAAGGAGAAAAAATATGCGTTTATTAGCCACATTAGTCAGCGTACTTGCGTTAAGCTTAAGCAGTAGTCTTGCTTATGCGAAATTTAAAGTTGTCACTACGTTCACTATTATTCAAGATATGGCACAAAATGTCGCCGGTGATGCGGCAACCGTAGAGTCCATCACCAAGCCCGGAGCAGAAATCCATGATTa                                                           <  1:141875/250‑1 (MQ=255)                                                                                                                                                                                          agaaagaaTATAATGTCCGCGCAGCTTATGTTGTTATTTTATAGTTAAAAAGCCAAGGAGAAAAAATATGCGTTTATTAGCCACATTAGTCAGCGTACTTGCGTTAAGCTTAAGCAGTAGTCTTGCTTATGCGAAATTTAAAGt                                                                                                                                                                 <  1:519217/144‑1 (MQ=255)                                                                                                                                                                                               gaaTATAATGTCCGCGCAGCTTATGTTGTTATTTTATAGTTAAAAAGCCAAGGAGAAAAAATATGCGTTTATTAGCCACATTAGTCAGCGTACTTg                                                                                                                                                                                                            <  1:831325/96‑1 (MQ=255)                                                                                                                                                                                               gaaTATAATGTCCGCGCAGCTTATGTTGTTATTTTATAGTTAAAAAGCCAAGGAGAAAAAATATGCGTTTATTAGCCACATTAGTCAGCGTACTTGCGTTAAGCTTAAGCAGTAGTCTTGCTTATGCGAAATTTAAAGTTGTCACTACGTTCACTATTATTCAAGATATGGCACAAAATGTCGCCGGTGATGCGGCAACCGTag                                                                                                >  1:574992/1‑204 (MQ=255)                                                                                                                                                                                               gaaTATAATGTCCGCGCAGCTTATGTTGTTATTTTATAGTTAAAAAGCCAAGGAGAAAAAATATGCGTTTATTAGCCACATTAGTCAGCGTACTTGCGTTAAGCTTAAGCAGTAGTCTTGCTTATGCGAAATTTAAAGTTGTCACTACGTTCACTATTATTCAAGATATGGCACAAAATGTCGCCGGTGATGCGGCAACCGTAGAGTCCATCACCAAGCCCGGAGCAGAAATCCATGATTACGAGCCAAc                                                  >  1:738033/1‑250 (MQ=255)                                                                                                                                                                                               gaaTATAATGTCCGCGCAGCTTATGTTGTTATTTTATAGTTAAAAAGCCAAGGAGAAAAAATATGCGTTTATTAGCCACATTAGTCAGCGTACTTGCGTTAAGCTTAAGCAGTAGTCTTGCTTATGCGAAATTTAAAGTTGTCACTACGTTCACTATTATTCAAGATATGGCACAAAATGTCGCCGGTGATGCGGCAACCGTAGAGTCCATCACCAAGCCCGGAGCAGAAATCCATGATTACGAGCCAAc                                                  >  1:789014/1‑250 (MQ=255)                                                                                                                                                                                                aaTATAATGTCCGCGCAGCTTATGTTGTTATTTTATAGTTAAAAAGCCAAGGAGAAAAAATATGCGTTTATTAGCCACATTAGTCAGCGTACTTGCGTTAAGCTTAAGCAGTAGTCTTGCTTATGCGAAATTTAAAGTTGTCACTACGTTCACTATTATTCAAGATATGGCACAAAATGTCGCCGGTGATGCGGCAACCGTAGAGTCCATCAc                                                                                      <  1:821665/213‑1 (MQ=255)                                                                                                                                                                                                  tataATGTCCGCGCAGCTTATGTTGTTATTTTATAGTTAAAAAGCCAAGGAGAAAAAATATGCGTTTATTAGCCACATTAGTCAGCGTACTTGCGTTAAGCTTAAGCAGTAGTCTTGCTTATGCGAAATTTAAAGTTGTCACTACGTTCACta                                                                                                                                                >  1:344732/1‑153 (MQ=255)                                                                                                                                                                                                  tataATGTCCGCGCAGCTTATGTTGTTATTTTATAGTTAAAAAGCCAAGGAGAAAAAATATGCGTTTATTAGCCACATTAGTCAGCGTACTTGCGTTAAGCTTAAGCAGTAGTCTTGCTTATGCGAAATTTAAAGTTGTCACTACGTTCACTATTATTCAAGATATGGCACAAAATGTCGCCGGTGATGCGGCAACCGTAGAGTCCATCACCAAGCCCGGAGCAGAAATCCATGATTACGAGCCAACCCCg                                              >  1:881016/1‑251 (MQ=255)                                                                                                                                                                                                      aTGTCCGCGCAGCTTATGTTGTTATTTTATAGTTAAAAAGCCAAGGAGAAAAAATATGCGTTTATTAGCCACATTAGTCAGCGTACTTGTGTTAAGCTTAAGCAGTAGTCTTGCTTATGCGAAATTTAAAGTTGTCACTACGTTCACTATTATTCAAGATATGGCACAAGATGTCGCCGGTGATGCGGCAACCGTAGAGt                                                                                             >  1:436007/1‑200 (MQ=255)                                                                                                                                                                                                       tGTCCGCGCAGCTTATGTTGTTATTTTATAGTTAAAAAGCCAAGGAGAAAAAATATGCGTTTATTAGCCACATTAGTCAGCGTACTTGCGTTAAGCTTAAGCAGTAGTCTTGCTTATGCGAAATTTAAAGTTGTCACTACGTTCACTATTATTc                                                                                                                                          <  1:457060/154‑1 (MQ=255)                                                                                                                                                                                                       tGTCCGCGCAGCTTATGTTGTTATTTTATAGTTAAAAAGCCAAGGAGAAAAAATATGCGTTTATTAGCCACATTAGTCAGCGTACTTGCGTTAAGCTTAAGCAGTAGTCTTGCTTATGCGAAATTTAAAGTTGTCACTACGTTCACTATTATTCAAGata                                                                                                                                    <  1:313714/160‑1 (MQ=255)                                                                                                                                                                                                       tGTCCGCGCAGCTTATGTTGTTATTTTATAGTTAAAAAGCCAAGGAGAAAAAATATGCGTTTATTAGCCACATTAGTCAGCGTACTTGCGTTAAGCTTAAGCAGTAGTCTTGCTTATGCGAAATTTAAAGTTGTCACTACGTTCACTATTATTCAAGATATGGCACAAAATGTCGCCGGTGATGCGGCAACCGTAGAGTCCATCACCAAGCCCGGAGCAGAAATCCATGATTACGAGCCAACCCCGAAAGa                                         >  1:25621/1‑251 (MQ=255)                                                                                                                                                                                                          ccGCGCAGCTTATGTTGTTATTTTATAGTTAAAAAGCCAAGGAGAAAAAATATGCGTTTATTAGCCACATTAGTCAGCGTACTTGCGTTAAGCTTAAGCAGTAGTCTTGCTTATGCGAAATTTAAAGTTGTCACTACGTTCACTATTATTCAAGATATGGCACAAAATGTCGCCGGTGATGCGGCAACCGTAGAGTCCATCACCAAGCCCGGAGCAGAAATCCATGATTACGAGCCAACCCCGAAAGatat                                      <  1:99059/251‑1 (MQ=255)                                                                                                                                                                                                               cAGCTTATGTTGTTATTTTATAGTTAAAAAGCCAAGGAGAAAAAATATGCGTTTATTAGCCACATTAGTCAGCGTACTTGCGTTAAGCTTAAGCAGTAGTCTTGCTTATGCGAAATTTAAAGTTGTCACTACGTTCACTATTATTCAAGATATGGCACAAAATGTCGCCGGTGATGCGGCAACCGTAGAGTCCATCAcc                                                                                     >  1:169650/1‑199 (MQ=255)                                                                                                                                                                                                                  cTTATGTTGTTATTTTATAGTTAAAAAGCCAAGGAGAAAAAATATGCGTTTATTAGCCACATTAGTCAGCGTACTTGCGTTAAGCTTAAGCAGTAGTCTTGCTTATGCGAAATTTAAAGTTGTCACTACGTTCACTATTATTCAAGATATGGCACAAAATGTCGCCGGTGATGCGGCAACCGTAGAGTCCATCACCAAGCCCGGAGCAGAAATCCATGATTACGAGCCAACCCCGAAAGATATTGTCAAgg                              >  1:589978/1‑251 (MQ=255)                                                                                                                                                                                                                      tgttgtTATTTTATAGTTAAAAAGCCAAGGAGAAAAAATATGCGTTTATTAGCCACATTAGTCAGCGTACTTGCGTTAAGCTTAAGCAGTAGTCTTGCTTATGCGAAATTTAAAGTTGTCACTACGTTCACTATTATTCAAGATATGGCACAAAATGTCGCCGGTGATGCGGCAACCGTAGAGTCCATCACCAAGCCCGGAGCAGAAATCCATGATTACGAGCCAACCCCGAAAGATATTGTCAAGGcaca                          >  1:435509/1‑251 (MQ=255)                                                                                                                                                                                                                          gttATTTTATAGTTAAAAAGCCAAGGAGAAAAAATATGCGTTTATTAGCCACATTAGTCAGCGTACTTGCGttaagcttaagc                                                                                                                                                                                              <  1:784212/83‑1 (MQ=255)                                                                                                                                                                                                                             aTTTTATAGTTAAAAAGCCAAGGAGAAAAAATATGCGTTTATTAGCCACATTAGTc                                                                                                                                                                                                                      <  1:228116/56‑1 (MQ=255)                                                                                                                                                                                                                             aTTTTATAGTTAAAAAGCCAAGGAGAAAAAATATGCGTTTATTAGCCACATTAGTCAGCGTACTTGCGTTAAGCTTAAGCAGTAGTCTTGCTTATGCGAAATTTAAAGTTGTCACTACGTTCACTATTATTCAAGATATGGCACAAAATGTCGCCGGTGATGCGGCAACCGTAGAGTCCATCACCAAGCCCGGAGCAGAAATCCATGATTACGAGCCAACCCCGAAAGATATTGTCAAGGCACAATCTGc                    >  1:901740/1‑250 (MQ=255)                                                                                                                                                                                                                             aTTTTATAGTTAAAAAGCCAAGGAGAAAAAATATGCGTTTATTAGCCACATTAGTCAGCGTACTTGCGTTAAGCTTAAGCAGTAGTCTTGCTTATGCGAAATTTAAAGATGTCACTACGTTCACTATTATTCAAGATATGGCACAAAATGTCGCCGGTGATGCGGCAACCGTAGAGTCCATCACCAAGCCCGGAGCAGAAATCCATGATTACGAGCCAACCCCGAAAGATATTGTCAAGGCACAATCTGc                    >  1:250197/1‑250 (MQ=255)                                                                                                                                                                                                                              ttttATAGTTAAAAAGCCAAGGAGAAAAAATATGCGTTTATTAGCCACATTAGTCAGCGTACTTGCGTTAAGCTTAAGCAGTAGTCTTGCTTATGCGAAATTTAAAGTTGTCACTACGTTCACTATTATTCAAGATATGGCACAAAATGTCGCCGGTGATGCGGCAACCGTAGAGTCCATCACCAAGCCCGGAGCAGAAATCCATGATTACGAGCCAACCCCGAAAGATATTGTCAAGGCACAATCTGCCg                  >  1:599987/1‑251 (MQ=255)                                                                                                                                                                                                                                     gTTAAAAAGCCAAGGAGAAAAAATATGCGTTTATTAGCCACATTAGTCAGCGTACTTGCGTTAAGCTTAAGCAGTAGTCTTGCTTATGCGAAATTTAAAGTTGTCACTACGTTCACTATTATTCAAGATATGGCACAAAATGTCGCCGGTGATGCGGCAACCGTAGAGTCCATCACCAAGCCCGGAGCAGAAATCCATGATTACGAGc                                                      <  1:93080/208‑1 (MQ=255)                                                                                                                                                                                                                                     gTTAAAAAGCCAAGGAGAAAAAATATGCGTTTATTAGCCACATTAGTCAGCGTACTTGCGTTAAGCTTAAGCAGTAGTCTTGCTTATGCGAAATTTAAAGTTGTCACTACGTTCACTATTATTCAAGATATGGCACAAAATGTCGCCGGTGATGCGGCAACCGTAGAGTCCATCACCAAGCCCGGAGCAGAAATCCATGATTACGAGCCAACCCCGAAAGATATTGTCAAGGCACAATCTGCCGATTTAgt           >  1:797512/1‑251 (MQ=255)                                                                                                                                                                                                                                              ccAAGGAGAAAAAATATGCGTTTATTAGCCACATTAGTCAGCGTACTTGCGTTAAGCTTAAGCAGTAGTCTTGCTTATGCGAAATTTAAAGTTGTCACTACGTTCACTATTATTCAAGATATGGCACAAAATGTCGCCGGTGATGCGGCAACCGTAGAGTCCATCACCAAGCCCGGAGCAGAAATCCATGATTACGAGCCAACCCCGAAAGATATTGTCAAGGCACAATCTGCCGATTTAGTGTTATGGaa  <  1:654075/251‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                   CCTCTCCTGCCATTCGTATGTTGGTGAAAGCCACCGCGCAAAAGCTGGGAGAAGATAAAGGCAATAGCCGTTATTTGCAACGTTTATTTCCCGATGGTCCGGCAAAACAGGCAACCAAGCTGGCGGGTCTCCCCAAACCGGTAAAATGTCTATAATTTTTTTGAGAATCATTTGCATTTAGAAATAGAAAGAATATAATGTCCGCGCAGCTTATGTTGTTATTTTATAGTTAAAAAGCCAAGGAGGAAAAATATGCGTTTATTAGCCACATTAGTCAGCGTACTTGCGTTAAGCTTAAGCAGTAGTCTTGCTTATGCGAAATTTAAAGTTGTCACTACGTTCACTATTATTCAAGATATGGCACAAAATGTCGCCGGTGATGCGGCAACCGTAGAGTCCATCACCAAGCCCGGAGCAGAAATCCATGATTACGAGCCAACCCCGAAAGATATTGTCAAGGCACAATCTGCCGATTTAGTGTTATGGAA  >  1/1814886‑1815373

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑