Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,590,185	A→G	intergenic (‑2976/+16339)	unknown ← / ← unknown	tRNA‑Val‑GAC/LSU rRNA ## 23S rRNA, large subunit ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,590,185	0	A	G	100.0%	196.6 / NA	60	intergenic (‑2976/+16339)	unknown/unknown	tRNA‑Val‑GAC/LSU rRNA ## 23S rRNA, large subunit ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (28/32);  total (28/32)

ATGATAAAAAGCGTATAGTGACACAGTTACCACGTCACTAAAACGGTTTGGCAAAATGAAATATCAAAAGTTAGAAAATCAAGAAGCCCATTGGAAATGGGCTTATTT‑AACGAAAAAAGCCCGTGAAGGCGAAAACGTTACCCGTTATGAAGAAAAAAGTTTGCAGGAAGACATCGTTAAGCAACTCATCGGCTGTCAAAATTACCCGCAAAAAATTGAAGAATGGATAAAAACCCATTTGGCACCGAAATTGGTGATCAAGCTAGACCAAGCCATTCGTGCCAGAAGAAAACGTTTTTTCAACGGTGAAAAACAATATACCAAGAAAAAATCCATTGATTTGGAATATGCCGTTTGGCTGCGTTTGTCCAAATATTCCCGCAAAATGAAAATGACGCTGTCGGAAACCATTTCCTACATGATTGACGAGCGCGAGAAAAAAGCCTTGTACGAAAATCAAATGTCCGCCATGAAAG  >  1/1589942‑1590417                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                         aTGATAAAAAGCGTATAGTGACACAGTTACCACGTCACTAAAACGGTTTGGCAAAATGAAATATCAAAAGTTAGAAAATCAAGAAGCCCATTGGAAATGGGCTTATTT‑AACGAAAAAAGCCCGTGAAGGCGAAAACGTTACCCGTTATGAAGAAAAAAGTTTGCAGGAAGACATCGTTAAGCAACTCATCGGCTGTCAAAATTACCCGCAAAAAATTGAAGAATGGATAAAAACCCATTTGGCGCCGAAAt                                                                                                                                                                                                                                   >  1:830994/1‑251 (MQ=255)                taGTGACACAGTTACCACGTCACTAAAACGGTTTGGCAAAATGAAATATCAAAAGTTAGAAAATCAAGAAGCCCATTGGAAATGGGCTTATTT‑AACGAAAAAAGCCCGTGAAGGCGAAAACGTTACCCGTTATGAAGAAAAAAGTTTGCAGGAAGACATCGTTAAGCAACTCATCGGCTGTCAAAATTACCCGCAAAAAATTGAAGAATGGATAAAAACCCATTTGGCGCCGAAATTGGTGATCAAGCTAg                                                                                                                                                                                                                    >  1:332347/1‑251 (MQ=255)                taGTGACACAGTTACCACGTCACTAAAACGGTTTGGCAAAATGAAATATCAAAAGTTAGAAAATCAAGAAGCCCATTGGAAATGGGCTTATTT‑AACGAAAAAAGCCCGTGAAGGCGAAAACGTTACCCGTTATGAAGAAAAAAGTTTGCAGGAAGACATCGTTAAGCAACTCATCGGCTGTCAAAATTACCCGCAAAAAATTGAAGAATGGATAAAAACCCATTTGGCGCCGAAATTGGTGATCAAGCTAg                                                                                                                                                                                                                    >  1:681067/1‑251 (MQ=255)                taGTGACACAGTTACCACGTCACTAAAACGGTTTGGCAAAATGAAATATCAAAAGTTAGAAAATCAAGAAGCCCATTGGAAATGGGCTTATTT‑AACGAAAAAAGCCCGTGAAGGCGAAAACGTTACCCGTTATGAAGAAAAAAGTTTGCAGGAAGACATCGTTAAGCAACTCATCGGCTGTCAAAATTACCCGCAAAAAATTGAAGAATGGATAAAAACCCATTTGGCGCCGAAATTGGTGATCAAGCTAg                                                                                                                                                                                                                    >  1:233095/1‑251 (MQ=255)                  gTGACACAGTTACCACGTCACTAAAACGGTTTGGCAAAATGAAATATCAAAAGTTAGAAAATCAAGAAGCCCATTGGAAATGGGCTTATTT‑AACGAAAAAAGCCCGTGAAGGCGAAAACGTTACCCGTTATGAAGAAAAAAGTTTGCAGGAAGACATCGTTAAGCAACTCATCGGCTGTCAAAATTACCCGCAAAAAATTGAAGAATGGATAAAAACCCATTTGGCGCCGAAATTGGTGATCAAGCTAGa                                                                                                                                                                                                                   >  1:227376/1‑250 (MQ=255)                  gTGACACAGTTACCACGTCACTAAAACGGTTTGGCAAAATGAAATATCAAAAGTTAGAAAATCAAGAAGCCCATTGGAAATGGGCTTATTT‑AACGAAAAAAGCCCGTGAAGGCGAAAACGTTACCCGTTATGAAGAAAAAAGTTTGCAGGAAGACATCGTTAAGCAACTCATCGGCTGTCAAAATTACCCGCAAAAAATTGAAGAATGGATAAAAACCCATTTGGCGCCGAAATTGGTGATCAAGCTAGa                                                                                                                                                                                                                   >  1:193176/1‑250 (MQ=255)                   tGACACAGTTACCACGTCACTAAAACGGTTTGGCAAAATGAAATATCAAAAGTTAGAAAATCAAGAAGCCCATTGGAAATGGGCTTATTT‑AACGAAAAAAGCCCGTGAAGGCGAAAACGTTACCCGTTATGAAGAAAAAAGTTTGCAGGAAGACATCGTTAAGCAACTCATCGGCTGTCAAAATTGCCCGCAAAAAATTGAAGAATGGATAAAAACCCATTTGGCGCCGAAATTGGTGATCAAGCTAGAcc                                                                                                                                                                                                                 <  1:855565/251‑1 (MQ=255)                   tGACACAGTTACCACGTCACTAAAACGGTTTGGCAAAATGAAATATCAAAAGTTAGAAAATCAAGAAGCCCATTGGAAATGGGCTTATTT‑AACGAAAAAAGCCCGTGAAGGCGAAAACGTTACCCGTTATGAAGAAAAAAGTTTGCAGGAAGACATCGTTAAGCAACTCATCGGCTGTCAAAATTACCCGCAAAAAATTGAAGAATGGATAAAAACCCATTTGGCGCCGAAATTGGTGATCAAGCTAGAcc                                                                                                                                                                                                                 <  1:220933/251‑1 (MQ=255)                   tGACACAGTTACCACGTCACTAAAACGGTTTGGCAAAATGAAATATCAAAAGTTAGAAAATCAAGAAGCCCATTGGAAATGGGCTTATTT‑AACGAAAAAAGCCCGTGAAGGCGAAAACGTTACCCGTTATGAAGAAAAAAGTTTGCAGGAAGACATCGTTAAGCAACTCATCGGCTGTCAAAATTACCCGCAAAAAATTGAAGAATGGATAAAAACCCATTTGGCGCCGAAATTGGTGATCAAGCTAGAcc                                                                                                                                                                                                                 <  1:163273/251‑1 (MQ=255)                   tGACACAGTTACCACGTCACTAAAACGGTTTGGCAAAATGAAATATCAAAAGTTAGAAAATCAAGAAGCCCATTGGAAATGGGCTTATTT‑AACGAAAAAAGCCCGTGAAGGCGAAAACGTTACCCGTTATGAAGAAAAAAGTTTGCAGGAAGACATCGTTAAGCAACTCATCGGCTGTCAAAATTACCCGCAAAAAATTGAAGAATGGATAAAAACCCATTTGGCGCCGAAATTGGTGATCAAGCTAGAc                                                                                                                                                                                                                  <  1:571886/250‑1 (MQ=255)                        caGTTACCACGTCACTAAAACGGTTTGGCAAAATGAAATATCAAAAGTTAGAAAATCAAGAAGCCCATTGGAAATGGGCTTATTT‑AACGAAAAAAGCCCGTGAAGGCGAAAACGTTACCCGTTATGAAGAAAAAAGTTTGCAGGAAGACATCGTTAAGCAACTCATCGGCTGTCAAAATTACCCGCAAAAAATTGAAGAATGGATAAAAACCCATTTGGCGCCGAAATTgg                                                                                                                                                                                                                                <  1:660669/231‑1 (MQ=255)                        caGTTACCACGTCACTAAAACGGTTTGGCAAAATGAAATATCAAAAGTTAGAAAATCAAGAAGCCCATTGGAAATGGGCTTATTT‑AACGAAAAAAGCCCGTGAAGGCGAAAACGTTACCCGTTATGAAGAAAAAAGTTTGCAGGAAGACATCGTTAAGCAACTCATCGGCTGTCAAAATTACCCGCAAAAAATTGAAGAATGGATAAAAACCCATTTGGCGCCGAAATTGGTGATCAAGCTAGAcc                                                                                                                                                                                                                 >  1:829976/1‑246 (MQ=255)                        caGTTACCACGTCACTAAAACGGTTTGGCAAAATGAAATATCAAAAGTTAGAAAATCAAGAAGCCCATTGGAAATGGGCTTATTT‑AACGAAAAAAGCCCGTGAAGGCGAAAACGTTACCCGTTATGAAGAAAAAAGTTTGCAGGAAGACATCGTTAAGCAACTCATCGGCTGTCAAAATTACCCGCAAAAAATTGAAGAATGGATAAAAACCCATTTGGCGCCGAAATTGGTGATCAAGCTAGAcc                                                                                                                                                                                                                 >  1:433588/1‑246 (MQ=255)                          gTTACCACGTCACTAAAACGGTTTGGCAAAATGAAATATCAAAAGTTAGAAAATCAAGAAGCCCATTGGAAATGGGCTTATTT‑AACGAAAAAAGCCCGTGAAGGCGAAAACGTTACCCGTTATGAAGAAAAAAGTTTGCAGGAAGACATCGTTAAGCAACTCATCGGCTGTCAAAATTACCCGCAAAAAATTGAAGAATGGATAAAAACCCATTTGGCGCCGAAATTGGTGATCAAGCTAGACCAAGCCAt                                                                                                                                                                                                          >  1:209162/1‑251 (MQ=255)                                      cTAAAACGGTTTGGCAAAATGAAATATCAAAAGTTAGAAAATCAAGAAGCCCATTGGAAATGGGCTTATTT‑AACGAAAAAAGCCCGTGAAGGCGAAAACGTTACCCGTTATGAAGAAAAAAGTTTGCAGGAAGACATCGTTAAGCAACTCATCGGCTGTCAAAATTACCCGCAAAAAATTGAAGAATGGATAAAAACCCATTTGGCGCCGAAATTGGTGATCAAGCTAGAcc                                                                                                                                                                                                                 <  1:85007/232‑1 (MQ=255)                                      cTAAAACGGTTTGGCAAAATGAAATATCAAAAGTTAGAAAATCAAGAAGCCCATTGGAAATGGGCTTATTT‑AACGAAAAAAGCCCGTGAAGGCGAAAACGTTACCCGTTATGAAGAAAAAAGTTTGCAGGAAGACATCGTTAAGCAACTCATCGGCTGTCAAAATTACCCGCAAAAAATTGAAGAATGGATAAAAACCCATTTGGCGCCGAAATTGGTGATCAAGCTAGACCAAGCCATTCGTGCCagaag                                                                                                                                                                                              >  1:742443/1‑251 (MQ=255)                                      cTAAAACGGTTTGGCAAAATGAAATATCAAAAGTTAGAAAATCAAGAAGCCCATTGGAAATGGGCTTATTT‑AACGAAAAAAGCCCGTGAAGGCGAAAACGTTACCCGTTATGAAGAAAAAAGTTTGCAGGAAGACATCGTTAAGCAACTCATCGGCTGTCAAAATTACCCGCAAAAAATTGAAGAATGGATAAAAACCCATTTGGCGCCGAAATTGGTGATCAAGCTAGACCAAGCCATTCGTGCCagaag                                                                                                                                                                                              <  1:38410/251‑1 (MQ=255)                                                  ggCAAAATGAAATATCAAAAGTTAGAAAATCAAGAAGCCCATTGGAAATGGGCTTATTT‑AACGAAAAAAGCCCGTGAAGGCGAAAACGTTACCCGTTATGAAGAAAAAAGTTTGCAGGAAGACATCGTTAAGCAACTCATCGGCTGTCAAAATTACCCGCAAAAAATTGAAGAATGGATAAAAACCCATTTGGCGCCGAAATTGGTGATCAAGCt                                                                                                                                                                                                                      >  1:484493/1‑215 (MQ=255)                                                         tGAAATATCAAAAGTTAGAAAATCAAGAAGCCCATTGGAAATGGGCTTATTT‑AACGAAAAAAGCCCGTGAAGGCGAAAACGTTACCCGTTATGAAGAAAAAAGTTTGCAGGAAGACATCGTTAAGCAACTCATCGGCTGTCAAAATTACCCGCAAAAAATTGAAGAATGGATAAAAACCCATTTGGCGCCGAAATTGGTGATCAAGCTAGACCAAGCCATTCGTGCCAGAAGAAAACGTTTTTTCAACGGt                                                                                                                                                                           >  1:89751/1‑251 (MQ=255)                                                               atCAAAAGTTAGAAAATCAAGAAGCCCATTGGAAATGGGCTTATTT‑AACGAAAAAAGCCCGTGAAGGCGAAAACGTTACCCGTTATGAAGAAAAAAGTTTGCAGGAAGACATCGTTAAGCAACTCATCGGCTGTCAAAATTACCCGCAAAAAATTGAAGAATGGATAAAAACCCATTTGGCGCCGAAATTGGTGATCAAGCt                                                                                                                                                                                                                      >  1:889594/1‑202 (MQ=255)                                                               atCAAAAGTTAGAAAATCAAGAAGCCCATTGGAAATGGGCTTATTT‑AACGAAAAAAGCCCGTGAAGGCGAAAACGTTACCCGTTATGAAGAAAAAAGTTTGCAGGAAGACATCGTTAAGCAACTCATCGGCTGTCAAAATTACCCGCAAAAAATTGAAGAATGGATAAAAACCCATTTGGCGCCGAAATTGGTGATCAAGCTAGACCAAGc                                                                                                                                                                                                             <  1:257651/211‑1 (MQ=255)                                                                tctaaaGTTAAAAAATCAAGAAACCCATTAAAAATGTGCGTATTTAAACAAAAAAAACCC‑TGAAAGCCAAAACAATACCCGTTATGAAGAAAAAAGTTTGCAGGAAGACATCGTTAAGCAACTCATCGGCTGTCAAAATTACCCGCAAAAAATTGAAGAATGGATAAAATCCCATTTGGCGCCGAAATTGGTGATCAAGCTAGACCAAGCCATTCGTGCCAGAAGAAAACGTTTTTTCAACGGTGAAAAAc                                                                                                                                                                    <  1:277101/248‑1 (MQ=255)                                                                tCAAAAGTTAGAAAATCAAGAAGCCCATTGGAAATGGGCTTATTT‑AACGAAAAAAGCCCGTGAAGGCGAAAACGTTACCCGTTATGAAGAAAAAAGTTTGCAGGAAGACATCGTTAAGCAACTCATCGGCTGTCAAAATTACCCGCAAAAAATTGAAGAATGGATAAAAACCCATTTGGCGCCGAAATTGGTGATCAAGCTAGACCAAGCCATTCGTGCCAGAAGAAAACGTTTTTTCAACGGTGAAAAAc                                                                                                                                                                    <  1:127626/251‑1 (MQ=255)                                                                       ttAGAAAATCAAGAAGCCCATTGGAAATGGGCTTATTT‑AACGAAAAAAGCCCGTGAAGGCGAAAACGTTACCCGTTATGAAGAAAAAAGTTTGCAGGAAGACATCGTTAAGCAACTCATCGGCTGTCAAAATTACCCGCAAAAAATTGAAGAATGGATAAAAACCCATTTGGCGCCGAAATTGGTGATCAAGCTAGACCAAGCCATTCGTGCCAGAAGAAAACGTTTTTTCAACGGTGAAAAACAatat                                                                                                                                                               <  1:174489/249‑1 (MQ=255)                                                                       ttAGAAAATCAAGAAGCCCATTGGAAATGGGCTTATTT‑AACGAAAAAAGCCCGTGAAGGCGAAAACGTTACCCGTTATGAAGAAAAAAGTTTGCAGGAAGACATCGTTAAGCAACTCATCGGCTGTCAAAATTACCCGCAAAAAATTGAAGAATGGATAAAAACCCATTTGGCGCCGAAATTGGTGATCAAGCTAGACCAAGCCATTCGTGCCAGAAGAAAACGTTTTTTCAACGGTGAAAAACAATATAc                                                                                                                                                             <  1:324676/251‑1 (MQ=255)                                                                       ttAGAAAATCAAGAAGCCCATTGGAAATGGGCTTATTT‑AACGAAAAAAGCCCGTGAAGGCGAAAACGTTACCCGTTATGAAGAAAAAAGTTTGCAGGAAGACATCGTTAAGCAACTCATCGGCTGTCAAAATTACCCGCAAAAAATTGAAGAATGGATAAAAACCCATTTGGCGCCGAAATTGGTGATCAAGCTAGACCAAGCCATTCGTGCCAGAAGAAAACGTTTTTTCAACGGTGAAAAACAATATAc                                                                                                                                                             <  1:54725/251‑1 (MQ=255)                                                                       ttAGAAAATCAAGAAGCCCATTGGAAATGGGCTTATTT‑AACGAAAAAAGCCCGTGAAGGCGAAAACGTTACCCGTTATGAAGAAAAAAGTTTGCAGGAAGACATCGTTAAGCAACTCATCGGCTGTCAAAATTACCCGCAAAAAATTGAAGAATGGATAAAAACCCATTTGGCGCCGAAATTGGTGATCAAGCTAGACCAAGCCATTCGTGCCAGAAGAAAACGTTTTTTCAACGGTGAAAAACAATATAc                                                                                                                                                             <  1:448931/251‑1 (MQ=255)                                                                              aTCAAGAAGCCCATTGGAAATGGGCTTATTT‑AACGAAAAAAGCCCGTGAAGGCGAAAACGTTACCCGTTATGAAGAAAAAAGTTTGCAGGAAGACATCGTTAAGCAACTCATCGGCTGTCAAAATTACCCGCAAAAAATTGAAGAATGGATAAAAACCCATTTGGCGCCGAAATTGGTGATCAAGCTAGACCAAGCCATTCGTGCCAGAAGAAAACGTTTTTTCAACGGTGAAAAACAATATACCAAGaaa                                                                                                                                                      >  1:255445/1‑251 (MQ=255)                                                                                cAAGAAGCCCATTGGAAATGGGCTTATTT‑AACGAAAAAAGCCCGTGAAGGCGAAAACGTTACCCGTTATGAAGAAAAAAGTTTGCAGGAAGACATCGTTAAGCAACTCATCGGCTGTCAAAATTACCCGCAAAAAATTGAAGAATGGATAAAAACCCATTTGGCGCCGAAATTGGTGATCAAGCTAGACCAAGCCATTCGTGCCAGAAGAAAACGTTTTTTCAACGGTGAAAAACAATATACCAAGaaaaa                                                                                                                                                    >  1:534287/1‑251 (MQ=255)                                                                                      gCCCATTGGAAATGGGCTTATTT‑AACGAAAAAAGCCCGTGAAGGCGAAAACGTTACCCGTTATGAAGAAAAAAGTTTGCAGGAAGACATCGTTAAGCAACTCATCGGCTGTCAAAATTACCCGCAAAAAATTGAAGAATGGATAAAAACCCATTTGGCGCCGAAATTGGTGATCAAGCTAGACCAAGCCATTCGTGCCAGAAGAAAACGTTTTTTCAACGGTGAAAAACAATATACCAAGAAAAAATCCAt                                                                                                                                              >  1:266641/1‑251 (MQ=255)                                                                                          aTTGGAAATGGGCTTATTT‑AACGAAAAAAGCCCGTGAAGGCGAAAACGTTACCCGTTATGAAGAAAAAAGTTTGCAGGAAGACATCGTTAAGCAACTCATCGGCTGTCAAAATTACCCGCAAAAAATTGAAGAATGGATAAAAACCCATTTGGCGCCGCAATTGGTGATCAAGCTAGACTAAGCCATTCGTGCCAGAAGAAAACGTTTTTTCAACGGTGAAAAACAATATACCAAGAAAAAATCCATTGAt                                                                                                                                          >  1:609807/1‑251 (MQ=255)                                                                                           ttGGAAATGGGCTTATTT‑AACGAAAAAAGCCCGTGAAGGCGAAAACGTTACCCGTTATGAAGAAAAAAGTTTGCAGGAAGACATCGTTAAGCAACTCATCGGCTGTCAAAATTACCCGCAAAAAATTGAAGAATGGATAAAAACCCATTTGGCGCCGAAATTGGTGATCAAGCTAGACCAAGCCATTCGTGCCAGAAGAAAACGTTTTTTCAACGGTGAAAAACAATATAcc                                                                                                                                                            >  1:285717/1‑232 (MQ=255)                                                                                           ttGGAAATGGGCTTATTT‑AACGAAAAAAGCCCGTGAAGGCGAAAACGTTACCCGTTATGAAGAAAAAAGTTTGCAGGAAGACATCGTTAAGCAACTCATCGGCTGTCAAAATTACCCGCAAAAAATTGAAGAATGGATAAAAACCCATTTGGCGCCGAAATTGGTGATCAAGCTAGACCAAGCCATTCGTGCCAGAAGAAAACGTTTTTTCAACGGTGAAAAACAATATACCAAGAAAAAATCCATTGAtt                                                                                                                                         >  1:252172/1‑251 (MQ=255)                                                                                              gAAATGGGCTTATTT‑AACGAAAAAAGCCCGTGAAGGCGAAAACGTTACCCGTTATGAAGAAAAAAGTTTGCAGGAAGACATCGTTAAGCAACTCATCGGCTGTCAAAATTACCCGCAAAAAATTGAAGAATGGATAAAAACCCATTTGGCGCCGAAATTGGTGATCAAGCTAGACCAAGCCATTCGTGCCAGAAGAAAACGTTTTTTCAAc                                                                                                                                                                              >  1:49094/1‑211 (MQ=255)                                                                                                aaTGGGCTTATTT‑AACGAAAAAAGCCCGTGAAGGCGAAAACGTTACCCGTTATGAAGAAAAAAGTTTGCAGGAAGACATCGTTAAGCAACTCATCGGCTGTCAAAATTACCCGCAAAAAATTGAAGAATGGATAAAAACCCATTTGGCGCCGAAATTGGTGATCAAGCTAGACCAAGCCATTCGTGCCAGAAGAAAACGTTTTTTCAACGGTGAAAAACAATATACCAAGAAAAAATCCATTGATTTGGaa                                                                                                                                    <  1:496636/251‑1 (MQ=255)                                                                                                aaTGGGCTTATTT‑AACGAAAAAAGCCCGTGAAGGCGAAAACGTTACCCGTTATGAAGAAAAAAGTTTGCAGGAAGACATCGTTAAGCAACTCATCGGCTGTCAAAATTACCCGCAAAAAATTGAAGAATGGATAAAAACCCATTTGGCGCCGAAATTGGTGATCAAGCTAGACCAAGCCATTCGTGCCAGAAGAAAACGTTTTTTCAACGGTGAAAAACAATATACCAAGAAAAAATCCATTGATTTGGaa                                                                                                                                    <  1:507854/251‑1 (MQ=255)                                                                                                aaTGGGCTTATTT‑AACGAAAAAAGCCCGTGAAGGCGAAAACGTTACCCGTTATGAAGAAAAAAGTTTGCAGGAAGACATCGTTAAGCAACTCATCGGCTGTCAAAATTACCCGCAAAAAATTGAAGAATGGATAAAAACCCATTTGGCGCCGAAATTGGTGATCAAGCTAGACCAAGCCATTCGTGCCAGAAGAAAACGTTTTTTCAACGGTGAAAAACAATATACCAAGAAAAAATCCATTGATTTGGaa                                                                                                                                    <  1:865125/251‑1 (MQ=255)                                                                                                aaTGGGCTTATTT‑AACGAAAAAAGCCCGTGAAGGCGAAAACGTTACCCGTTATGAAGAAAAAAGTTTGCAGGAAGACATCGTTAAGCAACTCATCGGCTGTCAAAATTACCCGCAAAAAATTGAAGAATGGATAAAAACCCATTTGGCGCCGAAATTGGTGATCAAGCTAGACCAAGCCATTCGTGCCAGAAGAAAACGTTTTTTCAACGGTGAAAAACAATATACCAAGAAAAAATCCATTGATTTGGaa                                                                                                                                    <  1:291417/251‑1 (MQ=255)                                                                                                    ggCTTATTT‑AACGAAAAAAGCCCGTGAAGGCGAAAACGTTACCCGTTATGAAGAAAAAAGTTTGCAGGAAGACATCGTTAAGCAACTCATCGGCTGTCAAAATTACCCGCAAAAAATTGAAGAATGGATAAAAACCCATTTGGCGCCGAAATTGGTGATCAAGCTAGACCAAGCCATTCGTGCCAGAAGAAAACGTTTTTTCAACGGTGAAAAAc                                                                                                                                                                    <  1:336856/215‑1 (MQ=255)                                                                                                              aaCGAAAAAAGCCCGTGAAGGCGAAAACGTTACCCGTTATGAAGAAAAAAGTTTGCAGGAAGACATCGTTAAGCAACTCATCGGCTGTCAAAATTACCCGCAAAAAATTGAAGAATGGATAAAAACCCATTTGGCGCCGAAATTGGTGATCAAGCTAGACCAAGCCATTCGTGCCAGAAGAAAACGTTTTTTCAACGGTGAAAAACAATATACCAAGAAAAAATCCATTGATTTGGAATATGCCGTTTGGc                                                                                                                       <  1:701997/251‑1 (MQ=255)                                                                                                               aCGAAAAAAGCCCGTGAAGGCGAAAACGTTACCCGTTATGAAGAAAAAAGTTTGCAGGAAGACATCGTTAAGCAACTCATCGGCTGTCAAAATTACCCGCAAAAAATTGAAGAATGGATAAAAACCCATTTGGCGCCGAAATTGGTGATCAAGCTAGACCAAGCCATTCGTGCCAGAAGAAAACGTTTTTTCAACGGTGAAAAACAATATACCAAGAAAAAATCCATTGATTTGGAATATGCCGTTTgg                                                                                                                        >  1:262943/1‑249 (MQ=255)                                                                                                                  aaaaaaGCCCGTGAAGGCGAAAACGTTACCCGTTATGAAGAAAAAAGTTTGCAGGAAGACATCGTTAAGCAACTCATCGGCTGTCAAAATTACCCGCAAAAAATTGAAGAATGGATAAAAACCCATTTGGCGCCGAAATTgg                                                                                                                                                                                                                                <  1:496353/142‑1 (MQ=255)                                                                                                                                  gCGAAAACGTTACCCGTTATGAAGAAAAAAGTTTGCAGGAAGACATCGTTAAGCAACTCATCGGCTGTCAAAATTACCCGCAAAAAATTGAAGAATGGATAAAAACCCATTTGGCGCCGAAATTGGTGATCAAGCTAGACCAAGCCATTCGTGCCAGAAGAAAACGTTTTTTCAACGGTGAAAAACAATATACCAAGAAAAAATCCATTGATTTGGAATATGCCGTTTGGCTGCGTTTGTCCAAATATTc                                                                                                    >  1:800027/1‑250 (MQ=255)                                                                                                                                         cGTTACCCGTTATGAAGAAAAAAGTTTGCAGGAAGACATCGTTAAGCAACTCATCGGCTGTCAAAATTACCCGCAAAAAATTGAAGAATGGATAAAAACCCATTTGGCGCCGAAATTGGTGATCAAGCTAGACCAAGCCATTCGTGCCAGAAGAAAACGTTTTTTCAACgg                                                                                                                                                                            <  1:490258/171‑1 (MQ=255)                                                                                                                                         cGTTACCCGTTATGAAGAAAAAAGTTTGCAGGAAGACATCGTTAAGCAACTCATCGGCTGTCAAAATTACCCGCAAAAAATTGAAGAATGGATAAAAACCCATTTGGCGCCGAAATTGGTGATCAAGCTAGACCAAGCCATTCGTGCCAGAAGAAAACGTTTTTTCAACGGTGAAAAAc                                                                                                                                                                    <  1:708537/179‑1 (MQ=255)                                                                                                                                                                    gCAGGAAGACATCGTTAAGCAACTCATCGGCTGTCAAAATTACCCGCAAAAAATTGAAGAATGGATAAAAACCCATTTGGCGCCGAAATTGGTGATCAAGCTAGACCAAGCCATTCGTGCCAGAAGAAAACGTTTTTTCAACGGTGAAAAACAATATACCAAGAAAAAATCCATTGATTTGGAATATGCCGTTTGGCTGCGTTTGTCCAAATATTCCCGCAAAATGAAAATGACGCTGTCGGAAACCAttt                                                                 >  1:310232/1‑251 (MQ=255)                                                                                                                                                                         aaGACATCGTTAAGCAACTCATCGGCTGTCAAAATTACCCGCAAAAAATTGAAGAATGGATAAAAACCCATTTGGCGCCGAAATTGGTGATCAAGCTAGACCAAGCCATTCGTGCCAGAAGAAAACGTTTTTTCAACGGTGAAAAACAATATACCAAGAAAAAATCCATTGATTTGGAATATGCCGTTTGGCTGCGTTTGTCCAAATATTCCCGCAAAATGAAAATGACGCTGTCGGAAACCATTTCCTa                                                             >  1:78718/1‑250 (MQ=255)                                                                                                                                                                               tCGTTAAGCAACTCATCGGCTGTCAAAATTACCCGCAAAAAATTGAAGAATGGATAAAAACCCATTTGGCGCCGAAATTGGTGATCAAGCTAGACCAAGCCATTCGTGCCAGAAGAAAACGTTTTTTCAACGGTGAAAAACAATATACCAAGAAAAAATCCATTGAtt                                                                                                                                         >  1:459087/1‑168 (MQ=255)                                                                                                                                                                               tCGTTAAGCAACTCATCGGCTGTCAAAATTACCCGCAAAAAATTGAAGAATGGATAAAAACCCATTTGGCGCCGAAATTGGTGATCAAGCTAGACCAAGCCATTCGTGCCAGAAGAAAACGTTTTTTCAACGGTGAAAAACAATATACCAAGAAAAAATCCATTGATTTGGaa                                                                                                                                    <  1:589068/173‑1 (MQ=255)                                                                                                                                                                               tCGTTAAGCAACTCATCGGCTGTCAAAATTACCCGCAAAAAATTGAAGAATGGATAAAAACCCATTTGGCGCCGAAATTGGTGATCAAGCTAGACCAAGCCATTCGTGCCAGAAGAAAACGTTTTTTCAACGGTGAAAAACAATATACCAAGAAAAAATCCATTGATTTGGAATATGCCg                                                                                                                             <  1:304809/180‑1 (MQ=255)                                                                                                                                                                                   tAAGCAACTCATCGGCTGTCAAAATTACCCGCAAAAAATTGAAGAATGGATAAAAACCCATTTGGCGCCGAAATTGGTGATCAAGCTAGACCAAGCCATTCGTGCCAGAAGAAAACGTTTTTTCAACGGTGAAAAACAATATACCAAGAAAAAATCCATTGATTTGGAATATGCCGTTTGGCTGCGTTTGTCCAAATATTCCCGCAAAATGAAAATGACGCTGTCGGAAACCATTTCCTACATGATTGACg                                                  <  1:494320/251‑1 (MQ=255)                                                                                                                                                                                   tAAGCAACTCATCGGCTGTCAAAATTACCCGCAAAAAATTGAAGAATGGATAAAAACCCATTTGGCGCCGAAATTGGTGATCAAGCTAGACCAAGCCATTCGTGCCAGAAGAAAACGTTTTTTCAACGGTGAAAAACAATATACCAAGAAAAAATCCATTGATTTGGAATATGCCGTTTGGCTGCGTTTGTCCAAATATTCCCGCAAAATGAAAATGACGCTGTCGGAAACCATTTCCTACATGATTGACg                                                  <  1:831499/251‑1 (MQ=255)                                                                                                                                                                                   tAAGCAACTCATCGGCTGTCAAAATTACCCGCAAAAAATTGAAGAATGGATAAAAACCCATTTGGCGCCGAAATTGGTGATCAAGCTAGACCAAGCCATTCGTGCCAGAAGAAAACGTTTTTTCAACGGTGAAAAACAATATACCAAGAAAAAATCCATTGATTTGGAATATGCCGTTTGGCTGCGTTTGTCCAAATATTCCCGCAAAATGAAAATGACGCTGTCGGAAACCATTTCCTACATGATTGACg                                                  <  1:41855/251‑1 (MQ=255)                                                                                                                                                                                      gCAACTCATCGGCTGTCAAAATTACCCGCAAAAAATTGAAGAATGGATAAAAACCCATTTGGCGCCGAAATTGGTGATCAAGCTAGACCAAGCCATTCGTGCCAGAAGAAAACGTTTTTTCAACGGTGAAAAAc                                                                                                                                                                    >  1:283858/1‑134 (MQ=255)                                                                                                                                                                                          cTCATCGGCTGTCAAAATTACCCGCAAAAAATTGAAGAATGGATAAAAACCCATTTGGCGCCGAAATTGGTGATCAAGCTAGACCAAGCCATTCGTGCCAGAAGAAAACGTTTTTTCAACGGTGAAAAACAATATACCAAGAAAAAATCCATTGATTTGGAATATGCCGTTTGGc                                                                                                                       <  1:624172/175‑1 (MQ=255)                                                                                                                                                                                          cTCATCGGCTGTCAAAATTACCCGCAAAAAATTGAAGAATGGATAAAAACCCATTTGGCGCCGAAATTGGTGATCAAGCTAGACCAAGCCATTCGTGCCAGAAGAAAACGTTTTTTCAACGGTGAAAAACAATATACCAAGAAAAAATCCATTGATTTGGAATATGCCGTTTGGCTGCGTTTGTCCAAATATTCCCGCAAAATGAAAATGACGCTGTCGGAAAc                                                                      <  1:662817/224‑1 (MQ=255)                                                                                                                                                                                                                      aaaTTGAAGAATGGATAAAAACCCATTTGGCGCCGAAATTGGTGATCAAGCTAGACCAAGCCATTCGTGCCAGAAGAAAACGTTTTTTCAACGGTGAAAAACAATATACCAAGAAAAAATCCATTGA‑TTGGAATATGCCGTTTGGCTGCGTTTGTCCAAATATTCCCGCCAAATGAAAATGACGCTGTCGGAAACCATTTCCTACATGATTGACGAGCGCGAGAAAAAAGCCTTGTACGAAAATCAAATGt              >  1:439991/1‑251 (MQ=255)                                                                                                                                                                                                                                   gATAAAAACCCATTTGGCGCCGAAATTGGTGATCAAGCTAGACCAAGCCATTCGTGCCAGAAGAAAACGTTTTTTCAACGGTGAAAAACAATATACCAAGAAAAAATCCATTGATTTGGaa                                                                                                                                    <  1:31813/121‑1 (MQ=255)                                                                                                                                                                                                                                   gATAAAAACCCATTTGGCGCCGAAATTGGTGATCAAGCTAGACCAAGCCATTCATGCCAGAAGAAAACGTTTTTTCAACGGTGAAAAACAATATACCAAGAAAAAATCCATTGATTTGGAATATGCCGTTTGGCTGCGTTTGTCCAAATATTCCCGCAAAATGAAAATGACGCTGTCGGAAACCATTTCCTACATGATTGACGAGCGCGAGAAAAAAGCCTTGTACGAAAATCAAATGTCCGCCATGAAAg  >  1:695293/1‑251 (MQ=255)                                                                                                                                                                                                                                           cccATTTGGCGCCGAAATTGGTGATCAAGCTAGACCAAGCCATTCGTGCCAGAAGAAAACGTTTTTTCAACGGTGAAAAACAATATACCAAGAAAAAATCCATTGATTTGGAATATGCCGTTTGGCTGCGTTTGTCCAAATATTCCCGCAAAATGAAAATGACGCTGTCGGAAACCATTTCCTACATGATTGACGAGCGCGAGAAAAAAGCCTTGTACGAAAATCAAATg               <  1:29058/230‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                         ATGATAAAAAGCGTATAGTGACACAGTTACCACGTCACTAAAACGGTTTGGCAAAATGAAATATCAAAAGTTAGAAAATCAAGAAGCCCATTGGAAATGGGCTTATTT‑AACGAAAAAAGCCCGTGAAGGCGAAAACGTTACCCGTTATGAAGAAAAAAGTTTGCAGGAAGACATCGTTAAGCAACTCATCGGCTGTCAAAATTACCCGCAAAAAATTGAAGAATGGATAAAAACCCATTTGGCACCGAAATTGGTGATCAAGCTAGACCAAGCCATTCGTGCCAGAAGAAAACGTTTTTTCAACGGTGAAAAACAATATACCAAGAAAAAATCCATTGATTTGGAATATGCCGTTTGGCTGCGTTTGTCCAAATATTCCCGCAAAATGAAAATGACGCTGTCGGAAACCATTTCCTACATGATTGACGAGCGCGAGAAAAAAGCCTTGTACGAAAATCAAATGTCCGCCATGAAAG  >  1/1589942‑1590417

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑