Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	277,921	A→G	intergenic (‑9639/+196155)	unknown ← / ← unknown	SSU rRNA ## 16S rRNA, small subunit ribosomal RNA/tRNA‑SeC(p)‑TCA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	277,921	0	A	G	100.0%	259.1 / NA	73	intergenic (‑9639/+196155)	unknown/unknown	SSU rRNA ## 16S rRNA, small subunit ribosomal RNA/tRNA‑SeC(p)‑TCA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (42/31);  total (42/31)

CATGTCGGTTTTTCTTTCTGTTCTGCCAATTCCAATGATGCTTTAATACGTGCTTGTTTCTTAGATTTTTCACGAACAGCAACTACATCATCAACTGAAACCTGGAAAGATGGAATGTTTACCACACGACCATTAACAACAATCGCTTTATGACTTACCAATTGGCGAGCCTCTGCGCGAGTGGCTGCGAACCCCATACGATAAACTATATTATCCAATCGACCTTCAAGCAACACTAATAAATTTTCACCTGTGTTACCCTTTAAGCGGTTTGCTTCTTTGTAGTAATTACGGAATTGGCGTTCCAAAATACCGTACATACGGCGAACTTTTTGTTTCTCACGCAACTGACTACCATAGTCAGACAGACGCGGTTTACGAGCACCATGTTGACCAGGCGCGATATCAATTTTACATTTTGAATCAATCGCACGAACACCTGATTTAAGAAATAAATCAGTGCCTTCACGGCGGCTTAGCTTGAGT  >  1/277680‑278165                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                     cATGTCGGTTTTTCTTTCTGTTCTGCCAATTCCAATGATGCTTTAATACGTGCTTGTTTCTTAGATTTTTCACGAACAGCAACTACATCATCAACTGAAACCTGGAAAGATGGAATGTTTACCACACGACCATTAACAACAATCGCTTTATGACTTACCAATTGGCGAGCCTCTGCGCGAGTGGCTGCGAACCCCATACGATAAACTATATTATCCAATCGACCTTCAAGCAACACTAATAGATTTTCAcc                                                                                                                                                                                                                                             <  1:287583/251‑1 (MQ=255)                  ctgttctgCCAATTCCAATGATGCTTTAATACGTGCTTGTTTCTTAGATTTTTCACGAACAGCAACTACATCATCAACTGAAACCTGGAAAGATGGAATGTTTACCACACGACCATTAACAACAATCGCTTTATGACTTACCAATTGGCGAGCCTCTGCGCGAGTGGCTGCGAACCCCATACGATAAACTATATTATCCAATCGACCTTCAAGCAACACTAATAGATTTTCACCTGTGTTAccc                                                                                                                                                                                                                                   >  1:833380/1‑244 (MQ=255)                  ctgttctgCCAATTCCAATGATGCTTTAATACGTGCTTGTTTCTTAGATTTTTCACGAACAGCAACTACATCATCAACTGAAACCTGGAAAGATGGAATGTTTACCACACGACCATTAACAACAATCGCTTTATGACTTACCAATTGGCGAGCCTCTGCGCGAGTGGCTGCGAACCCCATACGATAAACTATATTATCCAATCGACCTTCAAGCAACACTAATAGATTTTCACCTGTGTTACCCTTTAAGc                                                                                                                                                                                                                            <  1:623367/251‑1 (MQ=255)                  ctgttctgCCAATTCCAATGATGCTTTAATACGTGCTTGTTTCTTAGATTTTTCACGAACAGCAACTACATCATCAACTGAAACCTGGAAAGATGGAATGTTTACCACACGACCATTAACAACAATCGCTTTATGACTTACCAATTGGCGAGCCTCTGCGCGAGTGGCTGCGAACCCCATACGATAAACTATATTATCCAATCGACCTTCAAGCAACACTAATAGATTTTCACCTGTGTTACCCTTTAAGc                                                                                                                                                                                                                            <  1:302715/251‑1 (MQ=255)                  ctgttctgCCAATTCCAATGATGCTTTAATACGTGCTTGTTTCTTAGATTTTTCACGAACAGCAACTACACCATCAACTGAAACCTGGAAAGATGGAATGTTTACCACACGACCATTAACAACAATCGCTTTATGACTTACCAATTGGCGAGCCTCTGCGCGAGTGGCTGCGAACCCCATACGATAAACTATATTATCCAATCGACCTTCAAGCAACACTAATAGATTTTCACCTGTGTTACCCTTTAAGc                                                                                                                                                                                                                            <  1:605067/251‑1 (MQ=255)                              ttCCAATGATGCTTTAATACGTGCTTGTTTCTTAGATTTTTCACGAACAGCAACTACATCATCAACTGAAACCTGGAAAGATGGAATGTTTACCACACAACCATTAACAACAATCGCTTTATGACTTACCAATTGGCGAGCCTCTGCGCGAGTGGCTGCGAACCCCATACGATAAACTATATTATCCAATCGACCTTCAAGCAACACTAATAGATTTTCACCTGTGTTACCCTTTAAGCGGTTTGcttctt                                                                                                                                                                                                                >  1:278794/1‑251 (MQ=255)                                           ttAATACGTGCTTGTTTCTTAGATTTTTCACGAACAGCAACTACATCATCAACTGAAACCTGGAAAGATGGAATGTTTACCACACGACCATTAACAACAATCGCTTTATGACTTACCAATTGGCGAGCCTCTGCGCGAGTGGCTGCGAACCCCATACGATAAACTATATTATCCAATCGACCTTCAAGCAACACTAATAGATTTTCACCTGTGTTACCCTTTAAGCGGTTTGCTTCTTTGTAGTAATTACg                                                                                                                                                                                                   >  1:82306/1‑251 (MQ=255)                                            tAATACGTGCTTGTTTCTTAGATTTTTCACGAACAGCAACTACATCATCAACTGAAACCTGGAAAGATGGAATGTTTACCACACGACCATTAACAACAATCGCTTTATGACTTACCAATTGGCGAGCCTCTGCGCGAGTGGCTGCGAACCCCATACGATAAACTATATTATCCAATCGACCTTCAAGCAACACTAATAGATTTTCACCTGTGTTACCCTTTAAGCGGTTTGCTTCTTTGTAGTAATTACgg                                                                                                                                                                                                  >  1:39561/1‑251 (MQ=255)                                                              tAGATTTTTCACGAACAGCAACTACATCATCAACTGAAACCTGG‑AAGATGGAATGTTTACCACACGACCATTAACAACAATCGCTTTATGACTTACCAATTGGCGAGCCTCTGCGCGAGTGGCTGCGAACCCCATACGATAAACTATATTATCCAATCGACCTTCAAGCAACACTAATAGATTTTCACCTGTGTTACCCTTTAAGCGGTTTGCTTCTTTGTAGTAATTACGGAATTGGCGTTCCAAAATAc                                                                                                                                                                               <  1:136659/251‑1 (MQ=255)                                                                 aTTTTTCACGAACAGCAACTACATCATCAACTGAAACCTGGAAAGATGGAATGTTTACCACACGACCATTAACAACAATCGCTTTATGACTTACCAATTGGCGAGCCTCTGCGCGAGTGGCTGCGAACCCCATACGATAAACTATATTATCCAATCGACCTTCAAGCAACACTAATAGATTTTCACCTGTGTTACCCTTTAAGCGGTTTGCTTCTTTGTAGTAATTACGGAATTGGCGTTCCAAAATAc                                                                                                                                                                               <  1:668802/249‑1 (MQ=255)                                                                 aTTTTTCACGAACAGCAACTACATCATCAACTGAAACCTGGAAAGATGGAATGTTTACCACACGACCATTAACAACAATCGCTTTATGACTTACCAATTGGCGAGCCTCTGCGCGAGTGGCTGCGAACCCCATACGATAAACTATATTATCCAATCGACCTTCAAGCAACACTAATAGATTTTCACCTGTGTTACCCTTTAAGCGGTTTGCTTCTTTGTAGTAATTACGGAATTGGCGTTCCAAAATAc                                                                                                                                                                               <  1:674040/249‑1 (MQ=255)                                                                      tCACGAACAGCAACTACATCATCAACTGAAACCTGGAAAGATGGAATGTTTACCACACGACCATTAACAACAATCGCTTTATGACTTACCAATTGGCGAGCCTCTGCGCGAGTGGCTGCGAACCCCATACGATAAACTATATTATCCAATCGACCTTCAAGCAACACTAATAGATTTTCACCTGTGTTACCCTTTAAGCGGTTTGCTTCTTTGTAGTAATTACGGAATTGGCGTTCCAAAATACCGTACAt                                                                                                                                                                        <  1:260083/251‑1 (MQ=255)                                                                      tCACGAACAGCAACTACATCATCAACTGAAACCTGGAAAGATGGAATGTTTACCACACGACCATTAACAACAATCGCTTTATGACTTACCAATTGGCGAGCCTCTGCGCGAGTGGCTGCGAACCCCATACGATAAACTATATTATCCAATCGACCTTCAAGCAACACTAATAGATTTTCACCTGTGTTACCCTTTAAGCGGTTTGCTTCTTTGTAGTAATTACGGAATTGGCGTTCCAAAATACCGTACAt                                                                                                                                                                        <  1:266747/251‑1 (MQ=255)                                                                        aCGAACAGAAACTACAACATCAACTGAATCCTGGAAAGATGGAATGTTTACCACACGGCCATTAACAACAATCGCTTTATGACTTACCAATTGGCGAGCCTCTGCGCGAGTGGCTGAGAACCCCATACGATAAACTATATTATCCAATCGACCTTCAAGCAACACTAATAGATTTTCACCTGTGTTACCCTTTAAGCGGTTTGCTTCTTTGTAGTAATTACGGAATTGGCGTTCCAAAATACCGTACATAc                                                                                                                                                                      <  1:838019/251‑1 (MQ=255)                                                                           aaCAGCAACTACATCATCAACTGAAACCTGGAAAGATGGAATGTTTACCACACGACCATTAACAACAATCGCTTTATGACTTACCAATTGGCGAGCCTCTGCGCGAGTGGCTGCGAACCCCATACGATAAACTATATTATCCAATCGACCTTCAAGCAACACTAATAGATTTTCACCTGTGTTACCCTTTAAGCGGTTTGCTTCTTTGTAGTAATTACGGAATTGGCGTTCCAAAATACCGTACATACgg                                                                                                                                                                    >  1:133847/1‑250 (MQ=255)                                                                            aCAGCAACTACATCATCAACTGAAACCTGGAAAGATGGAATGTTTACCACACGACCATTAACAACAATCGCTTTATGACTTACCAATTGGCGAGCCTCTGCGCGAGTGGCTGCGAACCCCATACGATAAACTATATTATCCAATCGACCTTCAAGCAACACTAATAGATTTTCACCTGTGTTACCCTTTAAGCGGTTTGcttc                                                                                                                                                                                                                  >  1:324420/1‑203 (MQ=255)                                                                             cAGCAACTACATCATCAACTGAAACCTGGAAAGATGGAATGTTTACCACACGACCATTAACAACAATCGCTTTATGACTTACCAATTGGCGAGCCTCTGCGCGAGTGGCTGCGAACCCCATACGATAAACTATATTATCCAATCGACCTTCAAGCAACACTAATAGATTTTCACCTGTGTTACCCTTTAAGCGGTTTGcttc                                                                                                                                                                                                                  >  1:799501/1‑202 (MQ=255)                                                                                   cTACATCATCAACTGAAACCTGGAAAGATGGAATGTTTACCACACGACCATTAACAACAATCGCTTTATGACTTACCAATTGGCGAGCCTCTGCGCGAGTGGCTGCGAACCCCATACGATAAACTATATTATCCAATCGACCTTCAAGCAACACTAATAGATTTTCACCTGTGTTAccc                                                                                                                                                                                                                                   >  1:676209/1‑179 (MQ=255)                                                                                   cTACATCATCAACTGAAACCTGGAAAGATGGAATGTTTACCACACGACCATTAACAACAATCGCTTTATGACTTACCAATTGGCGAGCCTCTGCGCGAGTGGCTGCGAACCCCATACGATAAACTATATTATCCAATCGACCTTCAAGCAACACTAATAGATTTTCACCTGTGTTACCCTTTAAGCGGTTTGCTTCTTTGTAGTAATTACGGAATTGGCGTTCCAAAATACCGTACATACGGCGAACtttt                                                                                                                                                           >  1:137682/1‑251 (MQ=255)                                                                                   cTACATCATCAACTGAAACCTGGAAAGATGGAATGTTTACCACACGACCATTAACAACAATCGCTTTATGACTTACCAATTGGCGAGCCTCTGCGCGAGTGGCTGCGAACCCCATACGATAAACTATATTATCCAATCGACCTTCAAGCAACACTAATAGATTTTCACCTGTGTTACCCTTTAAGCGGTTTGCTTCTTTGTAGTAATTACGGAATTGGCGTTCCAAAATACCGTACATACGGCGAACtttt                                                                                                                                                           >  1:65318/1‑251 (MQ=255)                                                                                              aCTGAAACCTGGAAAGATGGAATGTTTACCACACGACCATTAACAACAATCGCTTTATGACTTACCAATTGGCGAGCCTCTGCGCGAGTGGCTGCGAACCCCATACGATAAACTATATTATCCAATCGACCTTCAAGCAACACTAATAGATTTTCACCTGTGTTACCCTTTAAGCGGTTTGCTTCTTTGTAGTAATTACGGAATTGGCGTTCCAAAATACCGTACATACGGCGAACTTTTTGTTTCTCACg                                                                                                                                                >  1:259159/1‑251 (MQ=255)                                                                                                        ggAAAGATGGAATGTTTACCACACGACCATTAACAACAATCGCTTTATGACTTACCAATTGGCGAGCCTCTGCGCGAGTGGCTGCGAACCCCATACGATAAACTATATTATCCAATCGACCTTCAAGCAACACTAATAGATTTTCACCTGTGTTACCCTTTAAg                                                                                                                                                                                                                             >  1:794198/1‑164 (MQ=255)                                                                                                        ggAAAGATGGAATGTTTACCACACGACCATTAACAACAATCGCTTTATGACTTACCAATTGGCGAGCCTCTGCGCGAGTGGCTGCGAACCCCATACGATAAACTATATTATCCAATCGACCTTCAAGCAACACTAATAGATTTTCACCTGTGTTACCCTTTAAGCGGTTTGCTTCTTTGTAGTAATTACGGAATTGGCGTTCCAAAATACCGTACATACGGCGAACTTTTTGTTTCTCACGCAACTGACTa                                                                                                                                      >  1:513783/1‑251 (MQ=255)                                                                                                        ggAAAGATGGAATGTTTACCACACGACCATTAACAACAATCGCTTTATGACTTAACAATTGGCGAGCCTCTGCGCGAGTGGCTGCGAACCCCATACGATAAACTATATTATCCAATCGACCTTCAAGCAACACTAATAGATTTTCACCTGTGTTACCCTTTAAGCGGTTTGCTTCTTTGTAGTAATTACGGAATTGGCGTTCCAAAATACCGTACATACGGCGAACTTTTTGTTTCTCACGCAACTGACTa                                                                                                                                      >  1:123484/1‑251 (MQ=255)                                                                                                                 gAATGTTTACCACACGACCATTAACAACAATCGCTTTATGACTTACCAATTGGCGAGCCTCTGCGCGAGTGGCTGCGAACCCCATACGATAAACTATATTATCCAATCGACCTTCAAGCAACACTAATAGATTTTCACCTGTGTTACCCTTTAAGCGGTTTGCTTCTTTGTAGTAATTACGGAATTGGCGTTCCAAAATACCGTACATACGGCGAACTTTTTGTTTCTCACGCAACTGACTACCATAGTc                                                                                                                              <  1:331241/250‑1 (MQ=255)                                                                                                                    tGTTTACCACACGACCATTAACAACAATCGCTTTATGCCTTACCAATTGGCGAGCCTCTGCGCGAGTGGCTGCGAACCCCATACGATAAACTATATTATCCAATCGACCTTCAAGCAACACTAATAGATTTTCACCTGTGTTCCCCTTTAAGCGGTTTGCTTCTTTGTAGTAATTACGGAATTGGCGTTCCAAAATACCGTACATACGGCGAACTTTTTGTTTCTCACGCAACTGACTACCATAGTcagac                                                                                                                          <  1:338077/251‑1 (MQ=255)                                                                                                                     gTTTACCACACGACCATTAACAACAATCGCTTTATGTCTTACCAATTGGCGAGCCTCTACGCGAGTGGCTGCGAACCCCATACGATAAACTATATTATCCAATCGACCTTCAAGCAACACTAATAGATTTTCACCTGTGTTACCCTTTAAGCGGTTTGCTTCTTTGTAGTAATTACGGAATTGGCGTTCCAAAATACCGTACATACGGCGAACTTTTTGTTTCTCACGCAACTGACTACCATAGTcagaca                                                                                                                         >  1:499415/1‑251 (MQ=255)                                                                                                                     gTTTACCACACGACCATTAACAACAATCGCTTTATGACTTACCAATTGGCGAGCCTCTGCGCGAGTGGCTGCGAACCCCATACGATAAACTATATTATCCAATCGACCTTCAAGCAACACTAATAGATTTTCACCTGTGTTAccc                                                                                                                                                                                                                                   >  1:507869/1‑145 (MQ=255)                                                                                                                                 aCCATTAACAACAATCGCTTTATGACTTACCAATTGGCGAGCCTCTGCGCGAGTGGCTGCGAACCCCATACGATAAACTATATTATCCAATCGACCTTCAAGCAACACTAATAGATTTTCAcc                                                                                                                                                                                                                                             >  1:81207/1‑123 (MQ=255)                                                                                                                                 aCCATTAACAACAATCGCTTTATGACTTACCAATTGGCGAGCCTCTGCGCGAGTGGCTGCGAACCCCATACGATAAACTATATTATCCAATCGACCTTCAAGCAACACTAATAGATTTTCACCTGTGTTACCCTTTAAGCGGTTTGCTTCTTTGTAGTAATTACGGAATTGGCGTTCCAAAATACCGTACATACgg                                                                                                                                                                    >  1:325314/1‑196 (MQ=255)                                                                                                                                 aCCATTAACAACAATCGCTTTATGACTTACCAATTGGCGAGCCTCTGCGCGAGTGGCTGCGAACCCCATACGATAAACTATATTATCCAATCGACCTTCAAGCAACACTAATAGATTTTCACCTGTGTTACCCTTTAAGCGGTTTGCTTCTTTGTAGTAATTACGGAATTGGCGTTCCAAAATACCGTACATACGGCGAACTTTTTGTTTCTCACGCAACTGACTACCATAGTc                                                                                                                              >  1:552706/1‑234 (MQ=255)                                                                                                                                 aCCATTAACAACAATCGCTTTATGACTTACCAATTGGCGAGCCTCTGCGCGAGTGGCTGCGAACCCCATACGATAAACTATATTATCCAATCGACCTTCAAGCAACACTAATAGATTTTCACCTGTGTTACCCTTTAAGCGGTTTGCTTCTTTGTAGTAATTACGGAATTGGCGTTCCAAAATACCGTACATACGGCGAACTTTTTGTTTCTCACGCAACTGACTACCATAGTCAGACAGACGCGGTTTa                                                                                                              >  1:871880/1‑250 (MQ=255)                                                                                                                                  ccATTAACAACAATCGCTTTATGACTTACCAATTGGCGAGCCTCTGCGCGAGTGGCTGCGAACCCCATACGATAAACTATATTATCCAATCGACCTTCAAGCAACACTAATAGATTTTCAGCTGTGTTACCCTGTAAGCGGTTTGCTTCTTTGTAGTAATTACGGAATTGGCGTTCCAAAATACCGTACATACGGCGAACTTTTTGTTTCTCACGCAGCTGACTACCATAGTCAGACAGACGCGGTTTACg                                                                                                            <  1:525604/251‑1 (MQ=255)                                                                                                                                    aTTAACAACAATCGCTTTATGACTTACCAATTGGCGAGCCTCTGCGCGAGTGGCTGCGAACCCCATACGATAAACTATATTATCCAATCGACCTTCAAGCAACACTAATAGATTTTCACCTGTGTTACCCTTTAAGCGGTTTGCTTCTTTGTAGTAATTACGGAATTGGCGTTCCAAAATACCGTACATACGGCGAACTTTTTGTTTCTCACGCAACTGACTACCATAGTCAGACAGACGCGGTTTACGAg                                                                                                          >  1:601013/1‑251 (MQ=255)                                                                                                                                    aTTAACAACAATCGCTTTATGACTTACCAATTGGCGAGCCTCTGCGCGAGTGGCTGCGAACCCCATACGATAAACTATATTATCCAATCGACCTTCAAGCAACACTAATAGATTTTCACCTGTGTTACCCTTTAAGCGGTTTGCTTCTTTGTAGTAATTACGGAATTGGCGTTCCAAAATACCGTACATACGGCGAACTTTTTGTTTCTCACGCAACTGACTACCATAGTCAGACAGACGCGGTTTACGAg                                                                                                          >  1:384155/1‑251 (MQ=255)                                                                                                                                     ttAACAACAATCGCTTTATGACTTACCAATTGGCGAGCCTCTGCGCGAGTGGCTGCGAACCCCATACGATAAACTATATTATCCAATCGACCTTCAAGCAACACTAATAGATTTTCACCTGTGTTACCCTTTAAGCGGTTTGCTTCTTTGTAGTAATTACGGAATTGGCGTTCCAAAATACCGTACATACGGCGAACTTTTTGTTTCTCACGCAACTGACTACCATAGTCAGACAGACGCGGTTTACGAGc                                                                                                         <  1:561/251‑1 (MQ=255)                                                                                                                                           acaaTCGCTTTATGACTTACCAATTGGCGAGCCTCTGCGCGAGTGGCTGCGAACCCCATACGATAAACTATATTATCCAATCGACCTTCAAGCAACACTAATAGATTTTCACCTGTGTTACCCTTTAAGCGGTTTGCTTCTTTGTAGTAATTACGGAATTGGCGTTCCAAAATACCGTACATACGGCGAACTTTTTGTTTCTCACGCAACTGACTACCATAGTCAGACAGACGCGGTTTACGAGCACCATg                                                                                                   <  1:855088/251‑1 (MQ=255)                                                                                                                                                  cTTTATGACTTACCAATTGGCGAGCCTCTGCGCGAGTGGCTGCGAACCCCATACGATAAACTATATTATCCAATCGACCTTCAAGCAACACTAATAGATTTTCACCTGTGTTACCCTTTAAGCGGTTTGCTTCTTTGTAGTAATTACGGaa                                                                                                                                                                                                >  1:736330/1‑151 (MQ=255)                                                                                                                                                  cTTTATGACTTACCAATTGGCGAGCCTCTGCGCGAGTGGCTGCGAACCCCATACGATAAACTATATTATCCAATCGACCTTCAAGCAACACTAATAGATTTTCACCTGTGTTACCCTTTAAGCGGTTTGCTTCTTTGTAGTAATTACGGAATTGGCGTTCCAAAATACCGTACATACGGCGAACTTTTTGTTTCTCACGCAACTGACTACCATAGTCAGACAGACGCGGTTTACGAGCACCATGTTGACCa                                                                                            >  1:113777/1‑251 (MQ=255)                                                                                                                                                          cTTACCAATTGGCGAGCCTCTGCGCGAGTGGCTGCGAACCCCATACGATAAACTATATTATCCAATCGACCTTCAAGCAACACTAATAGATTTTCACCTGTGTTACCCTTTAAGCGGTTTGCTTCTTTGTAGTAATTACGGAATTGGCGTTCCAAAATACCGTACATACGGCGAACTTTTTGTTTCTCACGCAACTGACTACCATAGTCAGACAGACGCGGTTTACGAGCACCATGTTGACCAGGCGCGat                                                                                    >  1:542581/1‑251 (MQ=255)                                                                                                                                                                        aGCCTCTGCGCGAGTGGCTGCGAACCCCATACGATAAACTATATTATCCAATCGACCTTCAAGCAACACTAATAGATTTTCACCTGTGTTACCCTTTAAGCGGTTTGCTTCTTTGTAGTAATTACGGAATTGGCGTTCCAAAATACCGTACATACGGCGAACTTTTTGTTTCTCACGCAACTGACTACCATAGTCAGACAGACGCGGTTTACGAGCACCATGTTGACCAGGCGCGATATCAATTTTACAt                                                                       <  1:857748/250‑1 (MQ=255)                                                                                                                                                                               gcgcGAGTGGCTGCGAACCCCATACGATAAACTATATTATCCAATCGACCTTCAAGCAACACTAATAGATTTTCACCTGTGTTACCCTTTAAGCGGTTTGCTTCTTTGTAGTAATTACGGAATTGGCGTTCCAAAATACCGTACATACGGCGAACTTTTTGTTTCTCACGCAACTGACTACCATAGTCAGACAGACGCGGTTTACGAGCACCATGTTGAcc                                                                                             >  1:641139/1‑221 (MQ=255)                                                                                                                                                                                           gCGAACCCCATACGATAAACTATATTATCCAATCGACCTTCAAGCAACACTAATAGATTTTCACCTGTGTTACCCTTTAAGCGGTTTGcttc                                                                                                                                                                                                                  >  1:231868/1‑92 (MQ=255)                                                                                                                                                                                                  ccATACGATAAACTATATTATCCAATCGACCTTCAAGCAACACTAATAGATTTTCACCTGTGTTACCCTTTAAGCGGTTTGCTTCTTTGTAGTAATTACGGAATTGGCGTTCCAAAAt                                                                                                                                                                                 >  1:798427/1‑118 (MQ=255)                                                                                                                                                                                                  ccATACGATAAACTATATTATCCAATCGACCTTCAAGCAACACTAATAGATTTTCACCTGTGTTACCCTTTAAGCGGTTTGCTTCTTTGTAGTAATTACGGAATTGGCGTTCCAAAATACCGTACAt                                                                                                                                                                        <  1:412192/127‑1 (MQ=255)                                                                                                                                                                                                  ccATACGATAAACTATATTATCCAATCGACCTTCAAGCAACACTAATAGATTTTCACCTGTGTTACCCTTTAAGCGGTTTGCTTCTTTGTAGTAATTACGGAATTGGCGTTCCAAAATACCGTACATACGGCGAACTTTTTGTTTCTCACGCAACTGACTACCATAGTCAGACAGACGCGGTTTACGAGCACCATGTTGACCAGGCGCGATATCAATTTTACATTTTGAATCAATCGCACGAACACCTGAt                                            >  1:207222/1‑251 (MQ=255)                                                                                                                                                                                                   cATACGATAAACTATATTATCCAATCGACCTTCAAGCAACACTAATAGATTTTCACCTGTGTTACCCTTTAAGCGGTTTGCTTCTTTGTAGTAATTACGGAATTGGCGTTCCAAAATACCGTACATACGGCGAACTTTTTGTTTCTCACGCAACTGACTACCATAGTCAGACAGACGCGGTTTACGAGCACCATGTTGACCAGGCGCGATATCAATTTTACATTTTGAATCAATCGCACGAACACCTGAtt                                           <  1:362659/251‑1 (MQ=255)                                                                                                                                                                                                      aCGATAAACTATATTATCCAATCGACCTTCAAGCAACACTAATAGATTTTCACCTGTGTTACCCTTTAAGCGGTTTGCTTCTTTgtag                                                                                                                                                                                                           >  1:747109/1‑88 (MQ=255)                                                                                                                                                                                                       cGATAAACTATATTATCCAATCGACCTTCAAGCAACACTAATAGATTTTCACCTGTGTTACCCTTTAAGCGGTTTGCTTCTTTGTAGTAATTACGGAATTGGCGTTCCAAAATACCGTACATACGGCGAACTTTTTGTTTCTCACGCAACTGACTACCATAGTCAGACAGACGCGGTTTACGAGCACCATGTTGACCAGGCGCGATATCAATTTTACATTTTGaat                                                                <  1:651390/226‑1 (MQ=255)                                                                                                                                                                                                           aaaCTATATTATCCAATCGGCCTTCAAGCAACACTAATAGATTTTCACCTGTGTTACCCTTTAAGCGGTTTGCTTCTTTGTAGTAATTACGGAATTGGCGTTCCAAAATACCGTACATACGGCGAACTTTTTGTTTCTCACGCAACTGACTACCATAGTCAGACAGACGCGGTTTACGAGCACCATGTTGACCAGGCGCGATATCAATTTTAc                                                                         <  1:316266/213‑1 (MQ=255)                                                                                                                                                                                                           aaaCTATATTATCCAATCGACCTTCAAGCAACACTAATAGATTTTCGCCTGTGTTACCCTTTAAGCGGTTTGCTTCTTTGTAGTAATTACGGAATTgg                                                                                                                                                                                            <  1:447316/98‑1 (MQ=255)                                                                                                                                                                                                           aaaCTATATTATCCAATCGACCTTCAAGCAACACTAATAGATTTTCACCTGTGTTACCCTTTAAGCGGTTTGCTTCTTTGTAGTAATTACGGAATTGGCGTTCCAAAATACCGTACATACGGCGAACTTTTTGTTTCTCACGCAACTGACTACCAt                                                                                                                                  <  1:485397/156‑1 (MQ=255)                                                                                                                                                                                                           aaaCTATATTATCCAATCGACCTTCAAGCAACACTAATAGATTTTCACCTGTGTTACCCTTTAAGCGGTTTGCTTCTTTGTAGTAATTACGGAATTGGCGTTCCAAAATACCGTACATACGGCGAACTTTTTGTTTCTCACGCAACTGACTACCATAGTCAGACAGACGCGGTTTACGAGCACCATGTTGACCAGGCGCGATATCAATTTTACATTTTGAATCAATCGCACGAACACCTGATTTAAGaaat                                   >  1:247569/1‑251 (MQ=255)                                                                                                                                                                                                              cTATATTATCCAATCGACCTTCAAGCAACACTAATAGATTTTCACCTGTGTTACCCTTTAAGCGGTTTGCTTCTTTGTAGTAATTACGGAATTGGCGTTCCAAAATACCGTACATACGGCGAACTTTTTGTTTCTCACGCAACTGACTACCATAGTCAGACAGACGCGGTTTACGAGCACCATGTTGACCAGGCGCGATATCAATTTTACATTTTGAATCAATCGCACGAacac                                                 >  1:138761/1‑234 (MQ=255)                                                                                                                                                                                                                atatTATCCAATCGACCTTCAAGCAACACTAATAGATTTTCACCTGTGTTACCCTTTAAGCGGTTTGCTTCTTTGTAGTAATTACGGAAt                                                                                                                                                                                               <  1:145994/90‑1 (MQ=255)                                                                                                                                                                                                                atatTATCCAATCGACCTTCAAGCAACACTAATAGATTTTCACCTGTGTTACCCTTTAAGCGGTTTGCTTCTTTGTAGTAATTACGGAATTGGCGTTCCAAAATACCGTACATACGGCGAACTTTTTGTTTCTCACGCAACTGACt                                                                                                                                       >  1:513769/1‑146 (MQ=255)                                                                                                                                                                                                                atatTATCCAATCGACCTTCAAGCAACACTAATAGATTTTCACCTGTGTTACCCTTTAAGCGGTTTGCTTCTTTGTAGTAATTACGGAATTGGCGTTCCAAAATACCGTACATACGGCGAACTTTTTGTTTCTCACGCAACTGACTACCATAGTCAGACAGACGCGGTTTACGAGCACCATGTTGACCAGGCGCGATATCAATTTTACATTTTGAATCAATCGCACGAACACCTGATTTa                                         >  1:659134/1‑240 (MQ=255)                                                                                                                                                                                                                atatTATCCAATCGACCTTCAAGCAACACTAATAGATTTTCACCTGTGTTACCCTTTAAGCGGTTTGCTTCTTTGTAGTAATTACGGAATTGGCGTTCCAAAATACCGTACATACGGCGAACTTTTTGTTTCTCACGCAACTGACTACCATAGTCAGACAGACGCGGTTTACGAGCACCATGTTGACCAGGCGCGATATCAATTTTACATTTTGAATCAATCGCACGAACACCTGATTTAAGaaataaat                               >  1:296441/1‑250 (MQ=255)                                                                                                                                                                                                                atatTATCCAATCGACCTTCAAGCAACACTAATAGATTTTCACCTGTGTTACCCTTTAAGCGGTTTGCTTCTTTGTAGTAATTACGGAATTGGCGTTCCAAAATACCGTACATACGGCGAACTTTTTGTTTCTCACGCAACTGACTACCATAGTCAGACAGACGCGGTTTACGAGCACCATGTTGACCAGGCGCGATATCAATTTTACATTTTGAATCAATCGCACGAACACCTGATTTAAGaaataaat                               >  1:676253/1‑250 (MQ=255)                                                                                                                                                                                                                atatTATCCAATCGACCTTCAAGCAACACTAATAGATTTTCACCTGTGTTACCCTTTAAGCGGTTTGCTTCTTTGTAGTAATTACGGAATTGGCGTTCCAAAATACCGTACATACGGCGAACTTTTTGTTTCTCACGCAACTGACTACCATAGTCAGACAGACGCGGTTTACGAGCACCATGTTGACCAGGCGCGATATCAATTTTACATTTTGAATCAATCGCACGAACACCTGATTTAAGaaataaat                               >  1:539194/1‑250 (MQ=255)                                                                                                                                                                                                                atatTATCCAATCGACCTTCAAGCAACACTAATAGATTTTCACCTGTGTTACCCTTTAAGCGGTTTGCTTCTTTGTAGTAATTACGGAATTGGCGTTCCAAAATACCGTACATACGGCGAACTTTTTGTTTCTCACGCAACTGACTACCATAGTCAGACAGACGCGGTTTACGAGCACCATGTTGACCAGGCGCGATATCAATTTTACATTTTGAATCAATCGCACGAACACCTGATTTAAGAAATAAATc                              <  1:495604/251‑1 (MQ=255)                                                                                                                                                                                                                     atCCAATCGACCTTCAAGCAACACTAATAGATTTTCACCTGTGTTACCCTTTAAGCGGTTTGCTTCTTTGTAGTAATTACGGAATTGGCGTTCCAAAATACCGTACATACGGCGAACTTTTTGTTTCTCACGCAACTGACTACCATAGTCAGACAGACGCGGTTTACGAGCACCATGTTGACCAgg                                                                                          <  1:664631/186‑1 (MQ=255)                                                                                                                                                                                                                     atCCAATCGACCTTCAAGCAACACTAATAGATTTTCACCTGTGTTACCCTTTAAGCGGTTTGCTTCTTTGTAGTAATTACGGAATTGGCGTTCCAAAATACCGTACATACGGCGAACTTTTTGTTTCTCACGCAACTGACTACCATAGTCAGACAGACGCGGTTTACGAGCACCATGTTGACCAGGCGCGATATCAATTTTACATTTTGAATCAATCGCACGAACACCTGATTTAAGAAATAAATCAGTGc                         <  1:339537/251‑1 (MQ=255)                                                                                                                                                                                                                          aTCGACCTTCAAGCAACACTAATAGATTTTCACCTGTGTTACCCTTTAAGCGGTTTGCTTCTTTGTAGTAATTACGGAATTGGCGTTCCAAAATAc                                                                                                                                                                               >  1:416221/1‑96 (MQ=255)                                                                                                                                                                                                                               ccTTCAAGCAACACTAATAGATTTTCACCTGTGTTACCCTTTAAGCGGTTTGCTTCTTTGTAGTAATTACGGAATTGGCGTTCCAAAATACCGTACATACGGCGAACTTTTTGTTTCTCACGCAACTGACTAc                                                                                                                                     <  1:313432/133‑1 (MQ=255)                                                                                                                                                                                                                                      gCAACACTAATAGATTTTCACCTGTGTTACCCTTTAAGCGGTTTGCTTCTTTGTAGTAATTACGGAATTGGCGTTCCAAAATACCGTACATACGGCGAACTTTTTGTTTCTCACGCAACTGACTACCATAg                                                                                                                                >  1:471154/1‑131 (MQ=255)                                                                                                                                                                                                                                       cAACACTAATAGATTTTCACCTGTGTTACCCTTTAAGCGGTTTGCTTCTTTGTAGTAATTACGGAATTGGCGTTCCAAAATACCGTACATACGGCGAACTTTTTGTTTCTCACGCAACTGACTACCATAGTCAGACAGACGCGGTTTACGAGCACCATGTTGACCAGGCGCGATATCAATTTTACATTTTGAATCAATCGCACGAACACCTGATTTAAGAAATAAATCAGTGCCTTCACGGCGGCTTAGCt       <  1:766746/251‑1 (MQ=255)                                                                                                                                                                                                                                         acacTAATAGATTTTCACCTGTGTTACCCTTTAAGCGGTTTGCTTCTTTGTAGTAATTACGGAATTGGCGTTCCAAAATACCGTACATACGGCGAACTTTTTGTTTCTCACGCAACTGACTACCATAGTCAGACAGACGCGGTTTACGAGCACCATGTTGACCAGGCGCGATATCAATTTTACATTTTGAATCAATCGCACGAACACCTGATTTAAGAAATAAATCAGTGCCTTCACGGCGGCTTAGCTTg     <  1:464858/251‑1 (MQ=255)                                                                                                                                                                                                                                          cacTAATAGATTTTCACCTGTGTTACCCTTTAAGCGGTTTGCTTCTTTGTAGTAATTACGGAATTGGCGTTc                                                                                                                                                                                       <  1:787668/72‑1 (MQ=255)                                                                                                                                                                                                                                          cacTAATAGATTTTCACCTGTGTTACCCTTTAAGCGGTTTGCTTCTTTGTAGTAATTACGGAATTGGCGTTCCAAAATACCGTACATACGGCGAACTTTTTGTTTCTCACGCAACTGACTACCATAGTCAGACAGACGCGGTTTACGAGCACCATGTTGACCAGGCGCGATATCAATTTTACATTTTGAATCAATCGCACGAACACCTGATTTAAGa                                      <  1:537417/217‑1 (MQ=255)                                                                                                                                                                                                                                          cacTAATAGATTTTCACCTGTGTTACCCTTTAAGCGGTTTGCTTCTTTGTAGTAATTACGGAATTGGCGTTCCAAAATACCGTACATACGGCGAACTTTTTGTTTCTCACGCAACTGACTACCATAGTCAGACAGACGCGGTTTACGAGCACCATGTTGACCAGGCGCGATATCAATTTTACATTTTGAATCAATCGCACGAACACCTGATTTAAGAAATAAATCAGTGCCTTCACGGCGGCTTAGCTTGa    >  1:807344/1‑251 (MQ=255)                                                                                                                                                                                                                                            cTAATAGATTTTCACCTGTGTTACCCTTTAAGCGGTTTGCTTCTTTGTAGTAATTACGGAATTGGCGTTCCAAAATACCGTAc                                                                                                                                                                          >  1:126875/1‑83 (MQ=255)                                                                                                                                                                                                                                            cTAATAGATTTTCACCTGTGTTACCCTTTAAGCGGTTTGCTTCTTTGTAGTAATTACGGAATTGGCGTTCCAAAATACCGTACATACGGCGAACTTTTTGTTTCTCACGCAACTGACTACCATAGTCAGACAGACGCGGTTTACGAGCACCATGTTGACCAGGCGCGATATCAATTTTACATTTTGAATCAATCGCACGAACACCTGATTTAAGAAATAAATCAGTGCCTTCACGGCGGCTTAGCTTGAGt  >  1:239477/1‑251 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                     CATGTCGGTTTTTCTTTCTGTTCTGCCAATTCCAATGATGCTTTAATACGTGCTTGTTTCTTAGATTTTTCACGAACAGCAACTACATCATCAACTGAAACCTGGAAAGATGGAATGTTTACCACACGACCATTAACAACAATCGCTTTATGACTTACCAATTGGCGAGCCTCTGCGCGAGTGGCTGCGAACCCCATACGATAAACTATATTATCCAATCGACCTTCAAGCAACACTAATAAATTTTCACCTGTGTTACCCTTTAAGCGGTTTGCTTCTTTGTAGTAATTACGGAATTGGCGTTCCAAAATACCGTACATACGGCGAACTTTTTGTTTCTCACGCAACTGACTACCATAGTCAGACAGACGCGGTTTACGAGCACCATGTTGACCAGGCGCGATATCAATTTTACATTTTGAATCAATCGCACGAACACCTGATTTAAGAAATAAATCAGTGCCTTCACGGCGGCTTAGCTTGAGT  >  1/277680‑278165

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑