Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	273,492	G→A	intergenic (‑5210/+200584)	unknown ← / ← unknown	SSU rRNA ## 16S rRNA, small subunit ribosomal RNA/tRNA‑SeC(p)‑TCA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	273,492	0	G	A	100.0%	273.3 / NA	79	intergenic (‑5210/+200584)	unknown/unknown	SSU rRNA ## 16S rRNA, small subunit ribosomal RNA/tRNA‑SeC(p)‑TCA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (33/46);  total (33/46)

TTTGAGTCCATTTGGGGGACAGAATGGAAAACGCGCAATTTACATGGACGCGTGCAACAAATTCAGAATAAAAGAATTGCCGGTTTGGGCGGGGTATTTCGCGGACAAATCTGGATGCCGCCAAATAAGCCGTTATTTTTTGATCCGATCCACTATTGCCAATATTGCCCACAGGAAAAAATCTGGCGTGGCGGAGTGCCTTTGCGTCACCGCACGTCGATCTTTCCTTCCGATATTGAAATTTTCGAGCAACAACAAGCCGATATTTTAATTTGCCATGAAGCGCCGAAACCACACCCTTCCGGCTTTGATGTGATCAATCAACTGGCAAAAAAATTAGGTGTAACACAAATCTATCATGGACATCACCACGAAAATTTTGATTACCGCACCTTACCTCAACAGAATTACCAAATTATCAACGTGGGATTTCGTAGCTTATGTGATGAAGCGGGAAATTATCTTTATAT  >  1/273261‑273730                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                               tttGAGTCCATTTGGGGGACAGAATGGAAAACGCGCAATTTACATGGACGCGTGCAACAAATTCAGAATAAAAGAATTGCCGGTTTGGGCGGGGTATTTCGCGGACAAATCTGGATGCCGCCAAATAAGCCGTTATTTTTTGATCCGATCCACTATTGCCAATATTGCCCACAGGAAAAAATCTGGCGTGGCGGAGTGCCTTTGCGTCACCGCACGTCGATCTTTCCTTCCAATATTGAAATTTTCGAGca                                                                                                                                                                                                                             >  1:840213/1‑251 (MQ=255)      gTCCATTTGGGGGACAGAATGGAAAACGCGCAATTTACATGGACGCGTGCAACAAATTCAGAATAAAAGAATTGCCGGTTTGGGCGGGGTATTTCGCGGACAAATCTGGATGCCGCCAAATAAGCCGTTATTTTTTGATCCGATCCACTATTGCCAATATTGCCCACAGGAAAAAATCTGGCGTGGCGGAGTGCCTTTGCGTCACCGCACGTCGATCTTTCCTTCCAATATTGAAATTTTCGAGcaacaa                                                                                                                                                                                                                         >  1:683894/1‑250 (MQ=255)       tCCATTTGGGGGACAGAATGGAAAACGCGCAATTTACATGGACGCGTGCAACAAATTCAGAATAAAAGAATTGCCGGTTTGGGCGGGGTATTTCGCGGACAAATCTGGATGCCGCCAAATAAGCCGTTATTTTTTGATCCGATCCACTATTGCCAATATTGCCCACAGGAAAAAATCTGGCGTGGCGGAGTGCCTTTGCGTCACCGCACGTCGATCTTTCCTTCCAATATTGAAATTTTCGAGcaacaac                                                                                                                                                                                                                        <  1:543334/250‑1 (MQ=255)          aTTTGGGGGACAGAATGGAAAACGCGCAATTTACATGGACGCGTGCAACAAATTCAGAATAAAAGAATTGCCGGTTTGGGCGGGGTATTTCGCGGACAAATCTGGATGCCGCCAAATAAGCCGTTATTTTTTGATCCGATCCACTATTGCCAATATTGCCCACAGGAAAAAATCTGGCGTGGCGGAGTGCCTTTGCGTCACCGCACGTCGATCTTTCCTTCCAATATTGAAATTTTCGAGCAACAACAAg                                                                                                                                                                                                                     >  1:241963/1‑250 (MQ=255)                   aCAGAATGGAAAACGCGCAATTTACATGGACGCGTGCAACAAATTCAGAATAAAAGAATTGCCGGTTTGGGCGGGGTATTTCGCGGACAAATCTGGATGCCGCCAAATAAGCCGTTATTTTTTGATCCGATCCACTATTGCCAATATTGCCCACAGGAAAAAATCTGGCGTGGCGGAGTGCCTTTGCGTCACCGCACGTCGATCTTTCCTTCCAATATTGAAATTTTCGAGCAACAACAAGCCGATAtttt                                                                                                                                                                                                           >  1:656057/1‑251 (MQ=255)                       aaTGGAAAACGCGCAATTTACATGGACGCGTGCAACAAATTCAGAATAAAAGAATTGCCGGTTTGGGCGGGGTATTTCGCGGACAAATCTGGATGCCGCCAAATAAGCCGTTATTTTTTGATCCGATCCACTATTGCCAATATTGCCCACAGGAAAAAATCTGGCGTGGCGGAGTGCCTTTGCGTCACCGCACGTCGATCTTTCCTTCCAATATTGAAATTTTCGAGCAACAACAAGCCGATATTTTaa                                                                                                                                                                                                         <  1:219897/249‑1 (MQ=255)                       aaTGGAAAACGCGCAATTTACATGGACGCGTGCAACAAATTCAGAATAAAAGAATTGCCGGTTTGGGCGGGGTATTTCGCGGACAAATCTGGATGCCGCCAAATAAGCCGTTATTTTTTGATCCGATCCACTATTGCCAATATTGCCCACAGGAAAAAATCTGGCGTGGCGGAGTGCCTTTGCGTCACCGCACGTCGATCTTTCCTTCCAATATTGAAATTTTCGAGCAACAACAAGCCGATATTTTAAtt                                                                                                                                                                                                       <  1:399633/251‑1 (MQ=255)                                      aTTTACATGGACGCGTGCAACAAATTCAGAATAAAAGAATTGCCGGTTTGGGCGGGGTATTTCGCGGACAAATCTGGATGCCGCCAAATAAGCCGTTATTTTTTGATCCGATCCACTATTGCCAATATTGCCCACAGGAAAAAATCTGGCGTGGCGGAGTGCCTTTGCGTCACCGCACGTCGATCTTTCCTTCCAATATTGAAATTTTCGAGCAACAACAAGCCGATATTTTAATTTGCCATGAAGCGCCg                                                                                                                                                                                        >  1:116267/1‑251 (MQ=255)                                           cATGGACGCGTGCAACAAATTCAGAATAAAAGAATTGCCGGTTTGGGCGGGGTGTTTCGCGGACAAATCTGGATGCCGCCAAATAAGCCGTTATTTTTTGATCCGATCCACTATTGCCAATATTGCCCACAGGAAAAAATCTGGCGTGGCGGAGTGCCTTTGCGTCACCGCACGTCGATCTTTCCTTCCAATATTGAAATTTTCGAGCAACAACAAGCCGATATTTTAATTTGCCATGAAGCGCCGAAAcc                                                                                                                                                                                   <  1:584378/251‑1 (MQ=255)                                                aCGCGTGCAACAAATTCAGAATAAAAGAATTGCCGGTTTGGGCGGGGTATTTCGCGGACAAATCTGGATGCCGCCAAATAAGCCGTTATTTTTTGATCCGATCCACTATTGCCAATATTGCCCACAGGAAAAAATCTGGCGTGGCGGAGTGCCTTTGCGTCACCGCACGTCGATCTTTCCTTCCAATATTGAAATTTTCGAGCAACAACAAGCCGATATTTTAATTTGCCATGAAGCGCCGAAACCACAcc                                                                                                                                                                              <  1:1615/251‑1 (MQ=255)                                                       caacaaATTCAGAATAAAAGAATTGCCGGTTTGGGCGGGGTATTTCGCGGACAAATCTGGATGCCGCCAAATAAGCCGTTATTTTTTGATCCGATCCACTATTGCCAATATTGCCCACAGGAAAAAATCTGGCGTGGCGGAGTGCCTTTGCGTCACCGCACGTCGATCTTTCCTTCCAATATTGAAATTTTCGAGCAACAACAAGCCGATATTTTAATTTGCCATGAAGCGCCGAAACCACAcc                                                                                                                                                                              <  1:455188/244‑1 (MQ=255)                                                               tCAGAATAAAAGAATTGCCGGTTTGGGCGGGGTATTTCGCGGACAAATCTGGATGCCGCCAAATAAGCCGTTATTTTTTGATCCGATCCACTATTGCCAATATTGCCCACAGGAAAAAATCTGGCGTGGCGGAGTGCCTTTGCGTCACCGCACGTCGATCTTTCCTTCCAATATTGAAATTTTCGAGCAACAACAAGCCGATATTTTAATTTGCCATGAAGCGCCGAAACCACACCCTTCCGGCTTTGAtg                                                                                                                                                               <  1:546993/251‑1 (MQ=255)                                                               tCAGAATAAAAGAATTGCCGGTTTGGGCGGGGTATTTCGCGGACAAATCTGGATGCCGCCAAATAAGCCGTTATTTTTTGATCCGATCCACTATTGCCAATATTGCCCACAGGAAAAAATCTGGCGTGGCGGAGTGCCTTTGCGTCACCGCACGTCGATCTTTCCTTCCAATATTGAAATTTTCGAGCAACAACAAGCCGATATTTTAATTTGCCATGAAGCGCCGAAACCACACCCTTCCGGCTTTGAtg                                                                                                                                                               <  1:704051/251‑1 (MQ=255)                                                                    aTAAAAGAATTGCCGGTTTGGGCGGGGTATTTCGCGGACAAATCTGGATGCCGCCAAATAAGCCGTTATTTTTTGATCCGATCCACTATTGCCAATATTGCCCACAGGAAAAAATCTGGCGTGGCGGAGTGCCTTTGCGTCACCGCACGTCGATCTTTCCTTCCAATATTGAAATTTTCGAGCAACAACAAGCCGATATTTTAATTTGCCATGAAGCGCCGAAACCACACCCTTCCGGCTTTGATGTGatc                                                                                                                                                          <  1:665536/251‑1 (MQ=255)                                                                    aTAAAAGAATTGCCGGTTTGGGCGGGGTATTTCGCGGACAAATCTGGATGCCGCCAAATAAGCCGTTATTTTTTGATCCGATCCACTATTGCCAATATTGCCCACAGGAAAAAATCTGGCGTGGCGGAGTGCCTTTGCGTCACCGCACGTCGATCTTTCCTTCCAATATTGAAATTTTCGAGCAACAACAAGCCGATATTTTAATTTGCCATGAAGCGCCGAAACCACACCCTTCCGGCTTTGATGTGatc                                                                                                                                                          <  1:484622/251‑1 (MQ=255)                                                                       aaaGAATTGCCGGTTTGGGCGGGGTATTTCGCGGACAAATCTGGATGCCGCCAAATAAGCCGTTATTTTTTGATCCGATCCACTATTGCCAATATTGCCCACAGGAAAAAATCTGGCGTGGCGGAGTGCCTTTGCGTCACCGCACGTCGATCTTTCCTTCCAATATTGAAATTTTCGAGCAACAACAAGCCGATATTTTAATTTGCCATGAAGCGCCGAAACCACACCCTTCCGGCTTTGATGTGatcaat                                                                                                                                                       <  1:245788/251‑1 (MQ=255)                                                                           aaTTGCCGGTTTGGGCGGGGTATTTCGCGGACAAATCTGGATGCCGCCAAATAAGCCGTTATTTTTTGATCCGATCCACTATTGCCAATATTGCCCACAGGAAAAAATCTGGCGTGGCGGAGTGCCTTTGCGTCACCGCACGTCGATCTTTCCTTCCAATATTGAAATTTTCGAGCAACAACAAGCCGATATTTTAATTTGCCATGAAGCGCCGAAACCACACCCTTCCGGCTTTGATGTGatcaatca                                                                                                                                                     <  1:307275/249‑1 (MQ=255)                                                                                  ggTTTGGGCGGGGTATTTCGCGGACAAATCTGGATGCCGCCAAATAAGCCGTTATTTTTTGATCCGATCCACTATTGCCAATATTGCCCACAGGAAAAAATCTGGCGTGGCGGAGTGCCTTTGCGTCACCGCACGTCGATCTTTCCTTCCAATATTGAAATTTTCGAGCAACAACAAGCCGATATTTTAATTTGCCATGAAGCGCCGAAACCACACCCTTc                                                                                                                                                                          >  1:259429/1‑221 (MQ=255)                                                                                  ggTTTGGGCGGGGTATTTCGCGGACAAATCTGGATGCCGCCAAATAAGCCGTTATTTTTTGATCCGATCCACTATTGCCAATATTGCCCACAGGAAAAAATCTGGCGTGGCGGAGTGCCTTTGCGTCACCGCACGTCGATCTTTCCTTCCAATATTGAAATTTTCGAGCAACAACAAGCCGATATTTTAATTTGCCATGAAGCGCCGAAACCACACCCTTCCGGCTTTGATGTGATCAATCAACTGGCaaa                                                                                                                                            >  1:770154/1‑251 (MQ=255)                                                                                            gggTATTTCGCGGACAAATCTGGATGCCGCCAAATAAGCCGTTATTTTTTGATCCGATCCACTATTGCCAATATTGCCCACAGGAAAAAATCTGGCGTGGCGGAGTGCCTTTGCGTCACCGCACGTCGATCTTTCCTTCCAATATTGAAATTTTCGAGCAACAACAAGc                                                                                                                                                                                                                    >  1:139551/1‑169 (MQ=255)                                                                                            gggTATTTCGCGGACAAATCTGGATGCCGCCAAATAAGCCGTTATTTTTTGATCCGATCCACTATTGCCAATATTGCCCACAGGAAAAAATCTGGCGTGGCGGAGTGCCTTTGCGTCACCGCACGTCGATCTTTCCTTCCAATATTGAAATTTTCGAGCAACAACAAGCCGATATTTTAATTTGCCATGAAGCGCCGAAAc                                                                                                                                                                                    <  1:288389/201‑1 (MQ=255)                                                                                            gggTATTCCGCGGACAAATCTGGATGCCGCCAAATAATCCGTTATTGTTTCATCTGATCCACTATTGCCAATATTGCCCACAGGAAAATATCTGGCGTGGCGGAGTGCCTTTGCGTCACCGCACTTCGATCTTTCCTTCCAAGATTGAAATTTTCGAGCAACAACAAGCCGATATTTTAATTTGCCATGAAGCGCCGAAACCACACCCTTCCGGCTTTGATGTGATCAATCAACTGGCAAAAAAATTAgg                                                                                                                                   <  1:707222/250‑1 (MQ=255)                                                                                             ggTATTTCGCGGACAAATCTGGATGCCGCCAAATAAGCCGTTATTTTTTGATCCGATCCACTATTGCCAATATTGCCCACAGGAAAAAATCTGGCGTGGCGGAGTGCCTTTGCGTCACCGCACGTCGATCTTTCCTTCCAATATTGAAATTTTCGAGCAACAACAAGCCGATATTTTAATTTGCCATGAAGCGCCGAAACCACACCCTTCCGGCTTTGATGTGATCAATCAACTGGCAAAAAAATTAGgt                                                                                                                                  <  1:448457/250‑1 (MQ=255)                                                                                                   tCGCGGACAAATCTGGATGCCGCCAAATAAGCCGTTATTTTTTGATCCGATCCACTATTGCCAATATTGCCCACAGGAAAAAATCTGGCGTGGCGGAGTGCCTTTGCGTCACCGCACGTCGATCTTTCCTTCCAATATTGAAATTTTCGAGCAACAACAAGCCGATATTTTAATTTGCCATGAAGCGCCGAAACCACACCCTTCCGGCTTTGATGTGATCAATCAACTGGCAAAAAAATTAGGTGTAacac                                                                                                                           <  1:132218/251‑1 (MQ=255)                                                                                                          cAAATCTGGATGCCGCCAAATAAGCCGTTATTTTTTGATCCGATCCACTATTGCCAATATTGCCCACAGGAAAAAATCTGGCGTGGCGGAGTGCCTTTGCGTCACCGCACGTCGATCTTTCCTTCCAATATTGAAATTTTCGAGCAACAACAAGCCGATATTTTAATTTGCCATGAAGCGCCGAAAcc                                                                                                                                                                                   <  1:214709/188‑1 (MQ=255)                                                                                                           aaaTCTGGATGCCGCCAAATAAGCCGTTATTTTTTGATCCGATCCACTATTGCCAATATTGCCCACAGGAAAAAATCTGGCGTGGCGGAGTGCCTTTGCGTCACCGCACGTCAATCTTTCCTTCCAATATTGAAATTTTCGAGCAACAACAAGCCGATATTTTAATTTGCCATGAAGCGCCGAAACCACACCCTTCCGGCTTTGATGTGATCAATCAACTGGCAAAAAAATTAGGTGTAACACAAATCTAt                                                                                                                   <  1:372475/251‑1 (MQ=255)                                                                                                             aTCTGGATGCCGCCAAATAAGCCGTTATTTTTTGATCCGATCCACTATTGCCAATATTGCCCACAGGAAAAAATCTGGCGTGGCGGAGTGCCTTTGCGTCACCGCACGTCGATCTTTCCTTCCAATATTGAAATTTTCGAGCAACAACAAGCCGATATTTTAATTTGCCATGAAGCGCCGAAACCACACCCTTCCGGCTTTGATGTGATCAATCAACTGGCAAAAAAATTAGGTGTAACACAAATCTATCa                                                                                                                 <  1:10407/251‑1 (MQ=255)                                                                                                                tGGATGCCGCCAAATAAGCCGTTATTTTTTGATCCGATCCACTATTGCCAATATTGCCCACAGGAAAAAATCTGGCGTGGCGGAGTGCCTTTGCGTCACCGCACGTCGATCTTTCCTTCCAATATTGAAATTTTCGAGCAACAACAAGCCGATATTTTAATTTGCCATGAAGCGCCGAAACCACACCCTTCCGGCTTTGATGTGATCAATCAACTGGCAAAAAAATTAGGTGTAACACAAATCTATCATgg                                                                                                              <  1:409688/251‑1 (MQ=255)                                                                                                                   aTGCCGCCAAATAAGCCGTTATTTTTTGATCCGATCCACTATTGCCAATATTGCCCACAGGAAAAAATCTGGCGTGGCGGAGTGCCTTTGCGTCACCGCACGTCGATCTTTCCTTCCAATATTGAAATTTTCGAGCAACAACAAGCCGATATTTTAATTTGCCATGAAGCGCCGAAACCACACCCTTCCGGCTTTGATGTGATCAATCAACTGGCAAAAAAATTAGGTGTAACACAAATCTATCATGGAc                                                                                                            <  1:637752/250‑1 (MQ=255)                                                                                                                   aTGCCGCCAAATAAGCCGTTATTTTTTGATCCGATCCACTATTGCCAATATTGCCCACAGGAAAAAATCTGGCGTGGCGGAGTGCCTTTGCGTCACCGCACGTCGATCTTTCCTTCCAATATTGAAATTTTCGAGCAACAACAAGCCGATATTTTAATTTGCCATGAAGCGCCGAAACCACACCCTTCCGGCTTTGATGTGATCAATCAACTGGCAAAAAAATTAGGTGTAACACAAATCTATCATGGAc                                                                                                            <  1:13951/250‑1 (MQ=255)                                                                                                                   aTGCCGCCAAATAAGCCGTTATTTTTTGATCCGATCCACTATTGCCAATATTGCCCACAGGAAAAAATCTGGCGTGGCGGAGTGCCTTTGCGTCACCGCACGTCGATCTTTCCTTCCAATATTGAAATTTTCGAGCAACAACAAGCCGATATTTTAATTTGCCATGAAGCGCCGAAACCACACCCTTCCGGCTTTGATGTGATCAATCAACTGGCAAAAAAATTAGGTGTAACACAAATCTATCATGGAc                                                                                                            <  1:766064/250‑1 (MQ=255)                                                                                                                      ccgccAAATAAGCCGTTATTTTTTGATCCGATCCACTATTGCCAATATTGCCCACAGGAAAAAATCTGGCGTGGCGGAGTGCCTTTGCGTCACCGCACGTCGATCTTTCCTTCCAATATTGAAAt                                                                                                                                                                                                                                      >  1:549082/1‑125 (MQ=255)                                                                                                                       cgccAAATAAGCCGTTATTTTTTGATCCGATCCACTATTGCCAATATTGCCCACAGGAAAAAATCTGGCGTGGCGGAGTGCCTTTGCGTCACCGCACGTCGATCTTTCCTTCCAATATTGAAATTTTCGAGCAACAACAAGCCGATATTTTAATTTGCCATGAAGCGCCGAAACCACACCCTTCCGGCTTTGATGTGATCAATCAACTGGCAAAAAAATTAGGTGTAACACAAATCTATCATGGACATcac                                                                                                       <  1:403529/251‑1 (MQ=255)                                                                                                                           aaaTAAGCCGTTATTTTTTGATCCGATCCACTATTGCCAATATTGCCCACAGGAAAAAATCTGGCGTGGCGGAGTGCCTTTGCGTCACCGCACGTCGATCTTTCCTTCCAATATTGAAATTTTCGAGCAACAACAAGCCGATATTTTAATTTGCCATGAAGCGCCGAAACCACACCCTTCCGGCTTTGATGTGATCAATCAACTGGCAAAAAAATTAGGTGTAACACAAATCTATCATGGACATCACCACg                                                                                                   >  1:130684/1‑251 (MQ=255)                                                                                                                                               atccgatccACTATTGCCAATATTGCCCACAGGAAAAAATCTGGCGTGGCGGAGTGCCTTTGCGTCACCGCACGTCGATCTTTCCTTCCAATATTGAAATTTTCGAGCAACAACAAGCCGATATTTTAATTTGCCATGAAGCGCCGAAACCACACCCTTCCGGCTTTGATGTGATCAATCAACTGGCAAAAAAATTAGGTGTAACACAAATCTATCATGGACATCACCACGAAAATTTTGATTACCGCAc                                                                                >  1:458598/1‑250 (MQ=255)                                                                                                                                                 ccgatccACTATTGCCAATATTGCCCACAGGAAAAAATCTGGCGTGGCGGAGTGCCTTTGCGTCACCGCACGTCGATCTTTCCTTCCAATATTGAAATTTTCGAGCAACAACAAGCCGATATTTTAATTTGCCATGAAGCGCCGAAACCACACCCTTCCGGCTTTGATGTGATCAATCAACTGGCAAAAAAATTAGGTGTAACACAAATc                                                                                                                      <  1:890568/210‑1 (MQ=255)                                                                                                                                                    atccACTATTGCCAATATTGCCCACAGGAAAAAATCTGGCGTGGCGGAGTGCCTTTGCGTCACCGCACGTCGATCTTTCCTTCCAATATTGAAATTTTCGAGCAACAACAAGCCGATATTTTAATTTGCCATGAAGCGCCGAAACCACACCCTTCCGGCTTTGATGTGATCAATCAACTGGCAAAAAAATTAGTTGTAACACAAATCTATCATGGACATCACCACGAAAATTTTGATTACCGCACCTTAc                                                                           >  1:624047/1‑250 (MQ=255)                                                                                                                                                        aCTATTGCCAATATTGCCCACAGGAAAAAATCTGGCGTGGCGGAGTGCCTTTGCGTCACCGCACGTCGATCTTTCCTTCCAATATTGAAATTTTCGAGCAACAACAAGCCGATATTTTAATTTGCCATGAAGCGCCGAAACCACACCCTTCCGGCTTTGATGTGATCAATCAACTGGCAAAAAAATTAGGTGTAACACAAATCTATCATGGACATCACCACGAAAATTTTGATTACCGCACCTTACCTc                                                                        >  1:650439/1‑249 (MQ=255)                                                                                                                                                        aCTATTGCCAATATTGCCCACAGGAAAAAATCTGGCGTGGCGGAGTGCCTTTGCGTCACCGCACGTCGATCTTTCCTTCCAATATTGAAATTTTCGAGCAACAACAAGCCGATATTTTAATTTGCCATGAAGCGCCGAAACCACACCCTTCCGGCTTTGATGTGATCAATCAACTGGCAAAAAAATTAGGTGTAACACAAATCTATCATGGACATCACCACGAAAATTTTGATTACCGCACCTTACCTCaa                                                                      >  1:614002/1‑251 (MQ=255)                                                                                                                                                         cTATTGCCAATATTGCCCACAGGAAAAAATCTGGCGTGGCGGAGTGCCTTTGCGTCACCGCACGTCGATCTTTCCTTCCAATATTGAAATTTTCGAGCAACAACAAGCCGATATTTTAATTTGCCATGAAGCGCCGAAACCACACCCTTCCGGCTTTGATGTGATCAATCAACTGGCAAAAAAATTAGGTGTAACACAAATCTATCATGGACATcac                                                                                                       >  1:104338/1‑217 (MQ=255)                                                                                                                                                         cTATTGCCAATATTGCCCACAGGAAAAAATCTGGCGTGGCGGAGTGCCTTTGCGTCACCGCACGTCGATCTTTCCTTCCAATATTGAAATTTTCGAGCAACAACAAGCCGATATTTTAATTTGCCATGAAGCGCCGAAACCACACCCTTCCGGCTTTGATGTGATCAATCAACTGGCAAAAAAATTAGGTGTAACACAAATCTATCATGGACATCACCACGAAAATTTTGATTACCGCACCTTACCTCaa                                                                      >  1:162014/1‑250 (MQ=255)                                                                                                                                                         cTATTGCCAATATTGCCCACAGGAAAAAATCTGGCGTGGCGGAGTGCCTTTGCGTCACCGCACGTCGATCTTTCCTTCCAATATTGAAATTTTCGAGCAACAACAAGCCGATATTTTAATTTGCCATGAAGCGCCGAAACCACACCCTTCCGGCTTTGATGTGATCAATCAACTGGCAAAAAAATTAGGTGTAACACAAATCTATCATGGACATCACCACGAAAATTTTGATTACCGCACCTTACCTCaa                                                                      >  1:264599/1‑250 (MQ=255)                                                                                                                                                         cTATTGCCAATATTGCCCACAGGAAAAAATCTGGCGTGGCGGAGTGCCTTTGCGTCACCGCACGTCGATCTTTCCTTCCAATATTGAAATTTTCGAGCAACAACAAGCCGATATTTTAATTTGCCATGAAGCGCCGAAACCACACCCTTCCGGCTTTGATGTGATCAATCAACTGGCAAAAAAATTAGGTGTAACACAAATCTATCATGGACATCACCACGAAAATTTTGATTACCGCACCTTACCTCAAc                                                                     <  1:354324/251‑1 (MQ=255)                                                                                                                                                           atggCCAATATTACCCACAGGAAAAAAGCTGGCATGGCGGGGTGCCTTTGCGTCACCGCACGTCGATCTTTCCTTCCAATATTGAAATTTTCGAGCAACAACAAGCCGATATTTTAATTTGCCATGAAGCGCCGAAACCACACCCTTCCGGCTTTGATGTGATCAATCAACTGGCAAAAAAATTAGGTGTAacac                                                                                                                           <  1:672638/192‑1 (MQ=255)                                                                                                                                                           aTTGCCAATATTGCCCACAGGAAAAAATCTGGCGTGGCGGAGTGCCTTTGCGTCACCGCACGTCGATCTTTCCTTCCAATATTGAAATTTTCGAGCAACAACAAGCCGATATTTTAATTTGCCATGAAGCGCCGAAACCACACCCTTCCGGCTTTGATGTGATCAATCAACTGGCAAAAAAATTAGGTGTAACACAAATCTATCATGGACATCACCACGAAAATTTTGATTACCGCACCTTACCTCAACAg                                                                   >  1:751860/1‑251 (MQ=255)                                                                                                                                                             tGCCAATATTGCCCACAGGAAAAAATCTGGCGTGGCGGAGTGCCTTTGCGTCACCGCACGTCGATCTTTCCTTCCAATATTGAAATTTTCGAGCAACAACAAGCCGATATTTTAATTTGCCATGAAGCGCCGAAACCACAccc                                                                                                                                                                             <  1:375080/143‑1 (MQ=255)                                                                                                                                                             tGCCAATATTGCCCACAGGAAAAAATCTGGCGTGGCGGAGTGCCTTTGCGTCACCGCACGTCGATCTTTCCTTCCAATATTGAAATTTTCGAGCAACAACAAGCCGATATTTTAATTTGCCATGAAGCGCCGAAACCACACCCTTCCGGCTTTGATGTGATCAATCAACTGGCAAAAAAATTAGGTGTAacac                                                                                                                           >  1:625928/1‑193 (MQ=255)                                                                                                                                                             tGCCAATATTGCCCACAGGAAAAAATCTGGCGTGGCGGAGTGCCTTTGCGTCACCGCACGTCGATCTTTCCTTCCAATATTGAAATTTTCGAGCAACAACAAGCCGATATTTTAATTTGCCATGAAGCGCCGAAACCACACCCTTCCGGCTTTGATGTGATCAATCAACTGGCAAAAAAATTAGGTGTAACACAAATCTATCATGGACATCACCACGAAAATTTTGATTACCGCACCTTACCTCAACAGaa                                                                 >  1:820012/1‑251 (MQ=255)                                                                                                                                                               ccAATATTGCCCACAGGAAAAAATCTGGCGTGGCGGAGTGCCTTTGCGTCACCGCACGTCGATCTTTCCTTCCAATATTGAAATTTTCGAGCAACAACAAGCCGATATTTTAATTTGCCATGAAGCGCCGAAACCACACCCTTCCGGCTTTGATGTGATCAATCAACTGGCAAAAAAATTAGGTGTAACACAAATCTATCATGGACATCACCACGAAAATTTTGATTACCGCACCTTACCTCAACAGAAt                                                                <  1:528686/250‑1 (MQ=255)                                                                                                                                                               ccAATATTGCCCACAGGAAAAAATCTGGCGTGGCGGAGTGCCTTTGCGTCACCGCACGTCGATCTTTCCTTCCAATATTGAAATTTTCGAGCAACAACAAGCCGATATTTTAATTTGCCATGAAGCGCCGAAACCACACCCTTCCGGCTTTGATGTGATCAATCAACTGGCAAAAAAATTAGGTGTAACACAAATCTATCATGGACATCACCACGAAAATTTTGATTACCGCACCTTACCTCAACAGAAt                                                                <  1:454193/250‑1 (MQ=255)                                                                                                                                                                  atatTGCCCACAGGAAAAAATCTGGCGTGGCGGAGTGCCTTTGCGTCACCGCACGTCGATCTTTCCTTCCAATATTGAAATTTTCGAGCAACAACAAGCCGATATTTTAATTTGCCATGAAGCGCCGAAACCACACCCTTCCGGCTTTGATGTGatcaatca                                                                                                                                                     <  1:71009/162‑1 (MQ=255)                                                                                                                                                                   tatTGCCCACAGGAAAAAATCTGGCGTGGCGGAGTGCCTTTGCGTCACCGCACGTCGATCTTTCCTTCCAATATTGAAATTTTCGAGCAACAACAAGCCGATATTTTAATTTGCCATGAAGCGCCGAAACCACACCCTTCCGGCTTTGATGTGATCAATCAACTGGCAAAAAAATTAGGTGTAACACAAATCTATCATGGACATCACCACGAAAATTTTGATTACCGCACCTTACCTCAACAGAATTACCa                                                           <  1:519123/251‑1 (MQ=255)                                                                                                                                                                            caGGAAAAAATCTGGCGTGGCGGAGTGCCTTTGCGTCACCGCACGTCGATCTTTCCTTCCAATATTGAAATTTTCGAGCAACAACAAGCCGATATTTTAATTTGCCATGAAGCGCCGAAACCACACCCTTCCGGCTTTGATGTGATCAATCAACTGGCAAAAAAATTAGGTGTAACACAAATCTATCATGGAc                                                                                                            >  1:632428/1‑193 (MQ=255)                                                                                                                                                                            caGGAAAAAATCTGGCGTGGCGGAGTGCCTTTGCGTCACCGCACGTCGATCTTTCCTTCCAATATTGAAATTTTCGAGCAACAACAAGCCGATATTTTAATTTGCCATGAAGCGCCGAAACCACACCCTTCCGGCTTTGATGTGATCAATCAACTGGCAAAAAAATTAGGTGTAACACAAATCTATCATGGACATCACCACGAAAATTTTGATTACCGCACCTTACCTCAACAGAAt                                                                >  1:319427/1‑237 (MQ=255)                                                                                                                                                                              ggAAAAAATCTGGCGTGGCGGAGTGCCTTTGCGTCACCGCACGTCGATCTTTCCTTCCAATATTGAAATTTTCGAGCAACAACAAGCCGATATTTTAATTTGCCATGAAGCGCCGAAACCACACCCTTCCGGCt                                                                                                                                                                     <  1:705908/134‑1 (MQ=255)                                                                                                                                                                              ggAAAAAATCTGGCGTGGCGGAGTGCCTTTGCGTCACCGCACGTCGATCTTTCCTTCCAATATTGAAATTTTCGAGCAACAACAAGCCGATATTTTAATTTGCCATGAAGCGCCGAAACCACACCCTTCCGGCTTTGAtg                                                                                                                                                               <  1:54741/140‑1 (MQ=255)                                                                                                                                                                              ggAAAAAATCTGGCGTGGCGGAGTGCCTTTGCGTCACCGCACGTCGATCTTTCCTTCCAATATTGAAATTTTCGAGCAACAACAAGCCGATATTTTAATTTGCCATGAAGCGCCGAAACCACACCCTTCCGGCTTTGATGTGATCAATCAACTGGCAAAAAAATTAGGTGTAACACAAATc                                                                                                                      <  1:173256/181‑1 (MQ=255)                                                                                                                                                                              ggAAAAAATCTGGCGTGGCGGAGTGCCTTTGCGTCACCGCACGTCGATCTTTCCTTCCAATATTGAAATTTTCGAGCAACAACAAGCCGATATTTTAATTTGCCATGAAGCGCCGAAACCACACCCTTCCGGCTTTGATGTGATCAATCAACTGGC‑AAAAAATTAGGTGTATCACAAATCTATCATGGACATCACCACGAAAATTTTGATTACCGCACCTTACCTCAACAGAATTACCAAATTATCAACGt                                               >  1:854381/1‑251 (MQ=255)                                                                                                                                                                                  aaaaTCTGGCGTGGCGGAGTGCCTTTGCGTCACCGCACGTCGATCTTTCCTTCCAATATTGAAATTTTCGAGCAACAACAAGCCGATATTTTAATTTGCCATGAAGCGCCGAAACCACACCCTTCCGGCTTTGATGTGATCAATCAACTGGCAAAAAAATTAGGTGTAACACAAATCTATCATGGACATCACCACGAAAATTTTGATTACCGCACCTTACCTCAACAGAATTACCAAATTATCAACGTggg                                            <  1:501422/251‑1 (MQ=255)                                                                                                                                                                                      tCTGGCGTGGCGGAGTGCCTTTGCGTCACCGCACGTCGATCTTTCCTTCCAATATTGAAATTTTCGAGCAACAACAAGCCGATATTTTAATTTGCCATGAAGCGCCGAAACCACACCCTTCCGGCTTTGATGTGATCAATCAACTGGCAAAAAAATTAGGTGTAACACAAATCTATCATGGACATCACCACGAAAATTTTGATTACCGCACCTTACCTCAACAGAATTACCAAATTATCAACGTGGGAttt                                        <  1:552744/251‑1 (MQ=255)                                                                                                                                                                                          gcgtggcgGAGTGCCTTTGCGTCACCGCACGTCGATCTTTCCTTCCAATATTGAAATTTTCGAGCAACAACAAGCCGATATTTTAATTTGCCATGAAGCGCCGAAACCACACCCTTCCGGCTTTGATGTGATCAATCAACTGGCAAAAAAATTAGGTGTAACACAAATCTATCATGGACATCACCACGAAAATTTTGATTACCGCACCTTACCTCAACAGAATTACCAAATTATCAACGTGGGATTTCGt                                     <  1:254145/250‑1 (MQ=255)                                                                                                                                                                                             tggcgGAGTGCCTTTGCGTCACCGCACGTCGATCTTTCCTTCCAATATTGAAATTTTCGAGCAACAACAAGCCGATATTTTAATTTGCCATGAAGCGCCGAAACCACACCCTTCCGGCTTTGATGTGATCAATCAACTGGCAAAAAAATTAGGTGTAacac                                                                                                                           >  1:321990/1‑161 (MQ=255)                                                                                                                                                                                             tggcgGAGTGCCTTTGCGTCACCGCACGTCGATCTTTCCTTCCAATATTGAAATTTTCGAGCAACAACAAGCCGATATTTTAATTTGCCATGAAGCGCCGAAACCACACCCTTCCGGCTTTGATGTGATCAATCAACTGGCAAAAAAATTAGGTGTAACACAAATCTATCATGGACATCACCACGAAAATTTTGATTACCGCACCTTACCTCAACAGAATTACCAAATTATCAACGTGGGATTTCGTAGc                                  <  1:371879/250‑1 (MQ=255)                                                                                                                                                                                             tggcgGAGTGCCTTTGCGTCACCGCACGTCGATCTTTCCTTCCAATATTGAAATTTTCGAGCAACAACAAGCCGATATTTTAATTTGCCATGAAGCGCCGAAACCACACCCTTCCGGCTTTGATGTGATCAATCAACTGGCAAAAAAATTAGGTGTAACACAAATCTATCATGGACATCACCACGAAAATTTTGATTACCGCACCTTACCTCAACAGAATTACCAAATTATCAACGTGGGATTTCGTAGCt                                 <  1:347522/251‑1 (MQ=255)                                                                                                                                                                                                  gAGTGCCTTTGCGTCACCGCACGTCGATCTTTCCTTCCAATATTGAAATTTTCGAGCAACAACAAGCCGATATTTTAATTTGCCATGAAGCGCCGAAACCACACCCTTCCGGCTTTGATGTGATCAATCAACTGGCAAAAAAATTAGGTGTAACACAAATCTATCATGGACATCACCACGAAAATTTTGATTACCGCACCTTACCTCAACAGAATTACCAAATTATCAACGTGGGATTTCGTAGCTTAtg                             <  1:698653/250‑1 (MQ=255)                                                                                                                                                                                                    gTGCCTTTGCGTCACCGCACGTCGATCTTTCCTTCCAATATTGAAATTTTCGAGCAACAACAAGCCGATATTTTAATTTGCCATGAAGCGCCGAAACCACACCCTTCCGGCTTTGATGTGATCAATCAACTGGCAAAAAAATTAGGTGTAACACAAATCTATCATGGACATCACCACGAAAATTTTGATTACCGCACCTTACCTCAACAGAATTACCAAATTATCAACGTGGGATTTCGTAGCTTATGtga                          >  1:603005/1‑251 (MQ=255)                                                                                                                                                                                                     tGCCTTTGCGTCACCGCACGTCGATCTTTCCTTCCAATATTGAAATTTTCGAGCAACAACAAGCCGATATTTTAATTTGCCATGAAGCGCCGAAACCACACCCTTCCGGCTTTGATGTGATCAATCAACTGGCAAAAAAATTAGGTGTAACACAAATCTATCATGGACATCACCACGAAAATTTTGATTACCGCACCTTACCTCAACAGAATTACCAAATTATCAACGTGGGATTTCGTAGCTTAtgtg                           <  1:233240/249‑1 (MQ=255)                                                                                                                                                                                                     tGCCTTTGCGTCACCGCACGTCGATCTTTCCTTCCAATATTGAAATTTTCGAGCAACAACAAGCCGATATTTTAATTTGCCATGAAGCGCCGAAACCACACCCTTCCGGCTTTGATGTGATCAATCAACTGGCAAAAAAATTAGGTGTAACACAAATCTATCATGGACATCACCACGAAAATTTTGATTACCGCACCTTACCTCAACAGAATTACCAAATTATCAACGTGGGATTTCGTAGCTTATGtga                          <  1:800276/250‑1 (MQ=255)                                                                                                                                                                                                            gCGTCACCGCACGTCGATCTTTCCTTCCAATATTGAAATTTTCGAGcaacaacaa                                                                                                                                                                                                                      <  1:200588/55‑1 (MQ=255)                                                                                                                                                                                                            gCGTCACCGCACGTCGATCTTTCCTTCCAATATTGAAATTTTCGAGCAACAACAAGCCGATATTTTAATTTGCCATGAAGCGCCGAAACCACACCCTTCCGGCTTTGATGTGATCAATCAACTGGCAAAAAAATTAGGTGTAACACAAATCTATCATGGAc                                                                                                            >  1:536405/1‑161 (MQ=255)                                                                                                                                                                                                               tCACCGCACGTCGATCTTTCCTTCCAATATTGAAATTTTCGAGCAACAACAAGCCGATATTTTAATTTGCCATGAAGCGCCGAAACCACACCCTTCCGGCTTTGATGTGATCAATCAACTGGCAAAAAAATTAGGTGTAACACAAATCTATCATGGACATCACCACGAAAATTTTGATTACCGCACCTTACCTc                                                                        <  1:225458/194‑1 (MQ=255)                                                                                                                                                                                                                cACCGCACGTCGATCTTTCCTTCCAATATTGAAATTTTCGAGCAACAACAAGCCGATATTTTAATTTGCCATGAAGCGCCGAAACCACACCCTTCCGGCTTTGATGTGATCAATCAACTGGCAAAAAAATTAGGTGTAACACAAATCTATCATGGACATCACCACGAAAATTTTGATTACCGCACCTTACCTc                                                                        >  1:808654/1‑193 (MQ=255)                                                                                                                                                                                                                 aCCGCACGTCGATCTTTCCTTCCAATATTGAAATTTTCGAGCAACAACAAGCCGATATTTTAATTTGCCATGAAGCGCCGAAACCACACCCTTCCGGCTTTGATGTGATCAATCAACTGGCAAAAAAATTAGGTGTAACACAAATCTATCATGGACATCACCACGAAAATTTTGATTACCGCACCTTAcc                                                                          >  1:73160/1‑190 (MQ=255)                                                                                                                                                                                                                 aCCGCACGTCGATCTTTCCTTCCAATATTGAAATTTTCGAGCAACAACAAGCCGATATTTTAATTTGCCATGAAGCGCCGAAACCACACCCTTCCGGCTTTGATGTGATCAATCAACTGGCAAAAAAATTAGGTGTAACACAAATCTATCATGGACATCACCACGAAAATTTTGATTACCGCACCTTACCTCAACAGAATTACCAAATTATCAACGTGGGATTTCGTAGCTTATGTGATGAAGCGGGAAAt             >  1:117920/1‑251 (MQ=255)                                                                                                                                                                                                                  ccGCACGTCGATCTTTCCTTCCAATATTGAAATTTTCGAGCAACAACAAGCCGATATTTTAATTTGCCATGAAGCGCCGAAACCACACCCTTCCGGCTTTGATGTGATCAATCAACTGGCAAAAAAATTAGGTGTAACACAAATCt                                                                                                                     >  1:577197/1‑146 (MQ=255)                                                                                                                                                                                                                           gATCTTTCCTTCCAATATTGAAATTTTCGAGCAACAACAAGCCGATATTTTAATTTGCCATGAAGCGCCGAAACCACACCCTTCCGGCTTTGATGTGATCAATCAACTGGCAAAAAAATTAGGTGTAACACAAATCTATCATGGACATCACCACGAAAATTTTGATTACCGCACCTTACCTCAACAGAATTACCAAATTATCAACGTGGGATTTCGTAGCTTATGTGATGAAGCGGGAAATTATCTTtata   >  1:532313/1‑251 (MQ=255)                                                                                                                                                                                                                            aTCTTTCCTTCCAATATTGAAATTTTCGAGCAACAACAAGCCGATATTTTAATTTGCCATGAAGCGCCGAAACCACACCCTTCCGGCTTTGATGTGATCAATCAACTGGCAAAAAAATTAGGTGTAACACAAATCTATCATGGACATCACCACGAAAATTTTGATTACCGCACCTTACCTCAACAGAATTACCAAATTATCAACGTGGGATTTCGTAGCTTATGTGATGAAGCGGGAAATTATCTTTatat  >  1:98733/1‑251 (MQ=255)                                                                                                                                                                                                                                ttccttccAATATTGAAATTTTCGAGCAACAACAAGCCGATATTTTAATTTGCCATGAAGCGCCGAAACCACACCCTTCCGGCTTTGATGTGATCAATCAACTGGCAAAAAAATTAGGTGTAACACAAATCTATCATGGACATCACCACGAAAATTTTGATTACCGCACCTTACCTCAAc                                                                     <  1:308/180‑1 (MQ=255)                                                                                                                                                                                                                                    ttccAATATTGAAATTTTCGAGCAACAACAAGCCGATATTTTAATTTGCCATGAAGCGCCGAAACCACACCCTTCCGGCTTTGATGTGATCAATCAACTGGCAAAAAAATTAGGTGTAACACAAATCTATCATGGACATCACCACGAAAATTTTGATTACCGCACCTTACCTCAACAGAATTACCAAATTATCAACGTGGGATTTCGTAGCTTATGTGATGAAGCGGGAAATTATCt        >  1:218810/1‑237 (MQ=255)                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                               TTTGAGTCCATTTGGGGGACAGAATGGAAAACGCGCAATTTACATGGACGCGTGCAACAAATTCAGAATAAAAGAATTGCCGGTTTGGGCGGGGTATTTCGCGGACAAATCTGGATGCCGCCAAATAAGCCGTTATTTTTTGATCCGATCCACTATTGCCAATATTGCCCACAGGAAAAAATCTGGCGTGGCGGAGTGCCTTTGCGTCACCGCACGTCGATCTTTCCTTCCGATATTGAAATTTTCGAGCAACAACAAGCCGATATTTTAATTTGCCATGAAGCGCCGAAACCACACCCTTCCGGCTTTGATGTGATCAATCAACTGGCAAAAAAATTAGGTGTAACACAAATCTATCATGGACATCACCACGAAAATTTTGATTACCGCACCTTACCTCAACAGAATTACCAAATTATCAACGTGGGATTTCGTAGCTTATGTGATGAAGCGGGAAATTATCTTTATAT  >  1/273261‑273730

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑