Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,481,625	G→T	intergenic (+149007/+105508)	unknown → / ← unknown	tRNA‑Met‑CAT/tRNA‑Val‑GAC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,481,625	0	G	T	100.0%	161.9 / NA	46	intergenic (+149007/+105508)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Val‑GAC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (21/25);  total (21/25)

ATGTGATAGTTTTGGGTGGACTGGCCGAAAACAAGATAACTGAAGGCGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTA‑TTTTCCGCTTGC‑GG‑AAAATGCAGGGCGAAGGCTTGCAGCCCGGTAAATAATGCTCGATGTTATTCGGGCATTTTTATTTGTTGCGCG‑CTGATGATTTTAATCGAGAAATGAAAATTTCCCGCCCCTGCAAATACCCCTAAAGCCACGTAATAACTGCTGATTAGATAATGCGATACGTGCTTGAATTTATGCTTACATTTTTAAATAAGTCTTGATAAATCAATATTTATTGATGTTACAAGGCTTTATTTCTTCAGTTTTCATAACGTTTAACT  >  1/1481394‑1481820                                                                                                                                                                                                                                           |                                                                                                                                                                                                     aTGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTgcgcgtacctaaatt                                                                                                                                                                                     >  1:212832/1‑241 (MQ=255)    tgattgttcttggtggtCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTgcgcgtacctaaatttaa                                                                                                                                                                                  >  1:137407/12‑238 (MQ=255)    tgattgttcttggtggtCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTgcgcgtacc                                                                                                                                                                                           <  1:57031/231‑5 (MQ=255)         gttcttggtggtCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTgcgcgtacctaaatttaatcgag                                                                                                                                                                             >  1:717953/7‑233 (MQ=255)         gttcttggtggtCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTgcgcgtacctaaatttaatcgag                                                                                                                                                                             >  1:322230/7‑233 (MQ=255)         gttcttggtggtCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTgcgcgtacctaaat                                                                                                                                                                                      <  1:275550/236‑10 (MQ=255)           tcttggtggtCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTgcgcgtacctaaatttaatcgagaa                                                                                                                                                                           >  1:203027/5‑231 (MQ=255)                   gtcTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTGCGCG‑TACCTAAATTTAATCGAGAAATGAAAAt                                                                                                                                                                  >  1:850041/3‑251 (MQ=255)                             aaataaaaTAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTGCGCG‑TACCTAAATTTAATCGAGAAATGAAAATTTCCCGccc                                                                                                                                                         >  1:712035/8‑250 (MQ=255)                                  aaaTAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTGCGCG‑TACCTAAATTTAATCGAGAAATGAAAATTTCCCGCCCCTGCaa                                                                                                                                                   <  1:689263/249‑1 (MQ=255)                                               tgAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTAA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTGCGCG‑TACCTAAATTTAATCGAGAAATGAAAATTTCCCGCCCCTGCAAATACCCCTAAAGc                                                                                                                                      <  1:484113/250‑1 (MQ=255)                                               tgAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTGGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTGCGCG‑TACCTAAATTTAATCGAGAAATGAAAATTTCCCGCCCCTGCAAATACCCCTAAAGc                                                                                                                                      <  1:575145/250‑1 (MQ=255)                                                      ggTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTGCGCG‑TACCTAAATTTAATCGAGAAATGAAAATTTCCCGCCCCTGCAAATACCCCTAAAGCCACGtaa                                                                                                                               >  1:875811/1‑251 (MQ=255)                                                          tttCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTGCGCG‑TACCTAAATTTAATCGAGAAATGAAAATTTCCCGCCCCTGCAAATACCCCTAAAGCCACGtaataa                                                                                                                            >  1:521567/1‑250 (MQ=255)                                                          tttCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTGCGCG‑TACCTAAATTTAATCGAGAAATGAAAATTTCCCGCCCCTGCAAATACCCCTAAAGCCACGTAATAAc                                                                                                                           <  1:41444/251‑1 (MQ=255)                                                             cATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTGCGCG‑TACCTAAATTTAATCGAGAAATGAAAATTTCCCGCCCCTGCAAATACCCCTAAAGCCACGTAATAActgc                                                                                                                        <  1:498703/251‑1 (MQ=255)                                                                      cTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTGCGCG‑TACCTAAATTTAATCGAGAAATGAAAATTTCCCGCCCCTGCAAATACCCCTAAAGCCACGTAATAACTGCTGATTAGAt                                                                                                               <  1:790901/251‑1 (MQ=255)                                                                              gtttgatagttttttaacgaaattGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTGCGCG‑TACCTAAATTTAATCGAGAAATGAAAATTTCCCGCCCCTGCAAATACCCCTAAAGCCACGTAATAACTGCTGATTAGATAATGCGAt                                                                                                       >  1:297496/24‑251 (MQ=255)                                                                                         aaaaTCCAAAAATGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTGCGCG‑TACCTAAATTTAATCGAGAAATGAAAATTTCCCGCCCCTGCAAATACCCCTAAAGCCACGTAATAACTGCTGATTAGATAATGCGATACGTGCTTGaa                                                                                            <  1:341661/251‑1 (MQ=255)                                                                                                     tGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTGCGCG‑TACCTAAATTTAATCGAGAAATGAAAATTTCCCGCCCCTGCAAATACCCCTAAAGCCACGTAATAAc                                                                                                                           >  1:400230/1‑208 (MQ=255)                                                                                                        tACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTGCGCG‑TACCTAAATTTAATCGAGAAATGAAAATTTCCCGCCCCTGCAAATACCCCTAAAGCCACGTAATAACTGCTGATTAGATAATGc                                                                                                          <  1:531319/222‑1 (MQ=255)                                                                                                        tACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTGCGCG‑TACCTAAATTTAATCGAGAAATGAAAATTTCCCGCCCCTGCAAATACCCCTAAAGCCACGTAATAACTGCTGATTAGATAATGCGATAc                                                                                                     >  1:303813/1‑227 (MQ=255)                                                                                                        tACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTGCGCG‑TACCTAAATTTAATCGAGAAATGAAAATTTCCCGCCCCTGCAAATACCCCTAAAGCCACGTAATAACTGCTGATTAGATAATGCGATACg                                                                                                    <  1:613501/228‑1 (MQ=255)                                                                                                         aCTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTGCGCG‑TACCTAAATTTAATCGAGAAATGAAAATTTCCCGCCCCTGCAAATACCCCTAAAGCCACGTAATAACTGCTGATTAGATAATGCGATACGTGCTTGAATTTATGCTTACAtttt                                                                            >  1:739068/1‑251 (MQ=255)                                                                                                          cTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTGCGCG‑TACCTAAATTTAATCGAGAAATGAAAATTTCCCGCCCCTGCAAATACCCCTAAAGCCACGTAATAACTGCTGATTAGATAATGCGATACGTGCTTGAATTTATGCTTACAttttt                                                                           >  1:756241/1‑251 (MQ=255)                                                                                                              gACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTgcgcgtacctaaatttaat                                                                                                                                                                                 >  1:89368/1‑132 (MQ=255)                                                                                                              gACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTGCGCG‑TACCTAAATTTAATCGAGAAATGAAAATTTCCCGCCCCTGCAAATACCCCTaa                                                                                                                                         <  1:723725/185‑1 (MQ=255)                                                                                                              gACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTGCGCG‑TACCTAAATTTAATCGAGAAATGAAAATTTCCCGCCCCTGCAAATACCCCTAAAGCCACGTAATAACTGCTGATTAGATAATGCGATACGTGCTTGAATTTa                                                                                        >  1:791023/1‑234 (MQ=255)                                                                                                                cAGATATAGTGATTTTCCTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCAGGCAGAATGTTATTCGGGCATTTTTATTTTTTCCGCG‑TCCATAAATTAAATCGAGAATTGAAAATTTCCCGCCCCTGCAAATACCCCTAAAGCCACGTAATAACTGCTGATTAGATAATGCGATAc                                                                                                     <  1:226758/219‑1 (MQ=255)                                                                                                                   atatAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTGCGCG‑TACCTAAATTTAATCGAGAAATGAAAATTTCCCGCCCCTGCAAATACCCCTAAAGCCACGTAATAACTGCTGATTAGAt                                                                                                               <  1:171824/206‑1 (MQ=255)                                                                                                                         tGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGGTTGCAGCCCGATAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTGCGCGTCCCA‑AAATTTAATCAAGAAATAAAAATTTACCGCCCCTGCAAATACCCCTAAAGCCACGTAATAACTGCTGATTAGATAATGCGATACGTGCTTGAATTTATGCTTACATTTTTAAATAAGTCTTGATa                                                            <  1:711642/251‑1 (MQ=255)                                                                                                                           aTTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTGCGCG‑TACCTAAATTTAATCGAGAAATGAAAATTTCCCGCCCCTGCAAATACCCCTAAAGCCACGTAATAAc                                                                                                                           >  1:799159/1‑186 (MQ=255)                                                                                                                           aTTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTGCGCG‑TACCTAAATTTAATCGAGAAATGAAAATTTCCCGCCCCTGCAAATACCCCTAAAGCCACGTAATAACTGCTGATTAGATAATGCGATACGTGCTTGAATTTATGCTTACATTTTTAAATAAGTc                                                                  <  1:426022/243‑1 (MQ=255)                                                                                                                           aTTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTGCGCG‑TACCTAAATTTAATCGAGAAATGAAAATTTCCCGCCCCTGCAAATACCCCTAAAGCCACGTAATAACTGCTGATTAGATAATGCGATACGTGCTTGAATTTATGCTTACATTTTTAAATAAGTCTTGATaaa                                                          <  1:268366/251‑1 (MQ=255)                                                                                                                            ttttACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTGCGCG‑TACCTAAATTTAATCGAGAAATGAAAATTTCCCGCCCCTGCAAATACCCCTAAAGCCACGTAATAACTGCTGATTAGATAATGCGATACGTGCTTGAATTTATGCTTACATTTTTAAATAAGTCTTGATAAAt                                                         >  1:784907/1‑251 (MQ=255)                                                                                                                                     aaGTGAAACTTGTCTAACTATTTTTGAGCAAGC‑TCAAAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTGCGCG‑TACCTAAATTTAATCGAGAAATGAAAATTTCCCGCCCCTGCAAATACCCCTAAAGCCACGTAATAACTGCTGATTAGATAATGCGATACGTGCTTGAATTTATGCTTACATTTTTAAATAAGTCTTGATAAATCAAtattta                                                >  1:844796/1‑251 (MQ=255)                                                                                                                                                     aactatttttgggcaagctcaaaaaTGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTGCGCG‑TACCTAAATTTAATCGAGAAATGAAAATTTCCCGCCCCTGCAAATACCCCTAAAGCCACGTAATAACTGCTGATTAGATAATGCGATACGTGCTTGAATTTATGCTTACATTTTTAAATAAGTCTTGATAAATCAATATTTATTGATGTTACAAgg                                  >  1:346280/22‑250 (MQ=255)                                                                                                                                                     aactatttttgagcaagctcaaaaaTGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTGCGCG‑TACCTAAATTTAATCGAGAAATGAAAATTTCCCGCCCCTGCAAATACCCCTAAAGCCACGTAATAACTGCTGATTAGATAATGCGATACGTGCTTGAATTTATGCTTACATTTTTAAATAAGTCTTGATAAATCAATATTTATTGATGTTACAAGGc                                 <  1:236940/230‑1 (MQ=255)                                                                                                                                                                     gctcaaaaaTGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTGCGCG‑TACCTAAATTTAATCGAGAAATGAAAATTTCCCGCCCCTGCAAATa                                                                                                                                                <  1:720040/119‑1 (MQ=255)                                                                                                                                                                        caaaaaTGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTGCGCG‑TACCTAAATTTAATCGAGAAATGAAAATTTCCCGCCCCTGCAAATACCCCTAAAg                                                                                                                                       <  1:233728/128‑1 (MQ=255)                                                                                                                                                                                     cGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTGCGCG‑TACCTAAATTTAATCGAGAAATGAAAATTTCCCGCCCCTGCAAATACCCCTAAAGCCACGTAATAACTGCTGATTAGATAATGCGATACGTGCTTGAATTTATGCTTACATTTTTAAATAAGTCTTGATAAATCAATATTTATTGATGTTACAAGGCTTTATTTCTTCAGTTTCCATCACGTTTAAcc  <  1:367120/250‑2 (MQ=255)                                                                                                                                                                                                 gCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTGCGCG‑TACCTAAATTTAATCGAGAAATGAAAATTTCCCGCCCCTGCAAATACCCCTAAAGCCACGTAATAACTGCTGATTAGATAATGCGATACGTGCTTGAATTTATGCTTACATTTTTAAATAAGTCTTGATAAATCAATATTTATTGATGTTACaa                                    <  1:828074/204‑1 (MQ=255)                                                                                                                                                                                                  cccGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTGCGCG‑TACCTAAATTTAATCGAGAAATGAAAATTTCCCGCCCCTGCAAATACCCCTAAAGCCACGTAATAACTGCTGATTAGATAATGCGATACGTGCTTGAATTTATGCTTACATTTTTAAATAAGTCTTGATAAATCAatat                                                   <  1:530159/188‑1 (MQ=255)                                                                                                                                                                                                                    aTGTTATTCGGGCATTTTTATTTTTTGCGCG‑TACCTAAATTTAATCGAGAAATGAAAATTTCCCGCCCCTGCAAATACCCCTAAAGc                                                                                                                                      >  1:791278/1‑87 (MQ=255)                                                                                                                                                                                                                    aTGTTATTCGGGCATTTTTATTTTTTGCGCG‑TACCTAAATTTAATCGAGAAATGAAAATTTCCCGCCCCTGCAAATACCCCTAAAGCCACGTAATAACTGCTGATTAGATAATGCGATACGTGCTTGAATTTATGc                                                                                     <  1:51778/136‑1 (MQ=255)                                                                                                                                                                                                                    aTGTTATTCGGGCATTTTTATTTTTTGCGAA‑TACCTAAATTTAACCGAGAAATGAAAATTTCCCGCCCCTGCAAATACCCCTAAAGCCACGTAATAACTGCTGATTAGATAATGCGATACGTGCTTGAATTTATGc                                                                                     <  1:785375/136‑1 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                                     ATGTGATAGTTTTGGGTGGACTGGCCGAAAACAAGATAACTGAAGGCGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTA‑TTTTCCGCTTGC‑GG‑AAAATGCAGGGCGAAGGCTTGCAGCCCGGTAAATAATGCTCGATGTTATTCGGGCATTTTTATTTGTTGCGCG‑CTGATGATTTTAATCGAGAAATGAAAATTTCCCGCCCCTGCAAATACCCCTAAAGCCACGTAATAACTGCTGATTAGATAATGCGATACGTGCTTGAATTTATGCTTACATTTTTAAATAAGTCTTGATAAATCAATATTTATTGATGTTACAAGGCTTTATTTCTTCAGTTTTCATAACGTTTAACT  >  1/1481394‑1481820

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑