Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,481,566	C→T	intergenic (+148948/+105567)	unknown → / ← unknown	tRNA‑Met‑CAT/tRNA‑Val‑GAC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,481,566	0	C	T	100.0%	187.6 / NA	58	intergenic (+148948/+105567)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Val‑GAC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (26/32);  total (26/32)

ACACTTATAAAACGCGACATTGTCACAGATGTAAGTGTAAAAAATGGAGATGTGATAGTTTTGGGTGGACTGGCCGAAAACAAGATAACTGAAGGCGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTA‑TTTTCCGCTTGC‑GG‑AAAATGCAGGGCGAAGGCTTGCAGCCCGGTAAATAATGCTCGATGTTATTCGGGCATTTTTATTTGTTGCGCG‑CTGATGATTTTAATCGAGAAATGAAAATTTCCCGCCCCTGCAAATACCCCTAAAGCCACGTAATAACTGCTGATTAGATAATGCGATACGTGCTTGAATTTATGCTTACATTTTTAAATAAGTCTTGATAAATCAATATTTATTGATGTTACAAGGC  >  1/1481345‑1481789                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                 acacTTATAAAACGCGACATTGTCACAGATGTAAGTGTAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTaaac                                                                                                                                                                                                       <  1:828510/251‑2 (MQ=255) acacTTATAAAACGCGACATTGTCACAGATGTAAGTGTAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTCTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTaaac                                                                                                                                                                                                       <  1:639394/251‑2 (MQ=255)  cacTTATAAAACGCGACATTGTCACAGATGTAAGTGTAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTaaacc                                                                                                                                                                                                      <  1:119271/251‑3 (MQ=255)   acTTATAAAACGCGACATTGTCACAGATGTAAGTGTAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTaaacca                                                                                                                                                                                                     <  1:283417/251‑4 (MQ=255)        taAAACGCGACATTGTCACAGATGTAAGTGTAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTc                                                                                                                                                                                                <  1:21991/251‑1 (MQ=255)          aaaCGCGACATTGTCACAGATGTAAGTGTAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTaaac                                                                                                                                                                                                       >  1:882242/1‑241 (MQ=255)            aCGCGACATTGTCACAGATGTAAGTGTAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATg                                                                                                                                                                                            <  1:871823/251‑1 (MQ=255)             cgcgACATTGTCACAGATGTAAGTGTAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGt                                                                                                                                                                                           <  1:796521/251‑1 (MQ=255)               cgACATTGTCACAGATGTAAGTGTAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTa                                                                                                                                                                                         <  1:624089/251‑1 (MQ=255)                      gTCACAGATGTAAGTGTAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCggg                                                                                                                                                                                   >  1:315815/1‑250 (MQ=255)                       tCACAGATGTAAGTGTAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGt                                                                                                                                                                                           <  1:92661/241‑1 (MQ=255)                       tCACAGATGTAAGTGTAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGc                                                                                                                                                                                  <  1:251811/250‑1 (MQ=255)                       tCACAGATGTAAGTGTAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCa                                                                                                                                                                                 >  1:321878/1‑251 (MQ=255)                            gATGTAAGTGTAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCAtttt                                                                                                                                                                             >  1:791560/1‑250 (MQ=255)                             aTGTAAGTGTAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTa                                                                                                                                                                           >  1:18069/1‑251 (MQ=255)                                tAAGTGTAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAGCCATGCTCGATGTTATTCGGGCATTTTTAttt                                                                                                                                                                        <  1:721810/251‑1 (MQ=255)                                                  aTGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTgcgcgtacctaaatt                                                                                                                                                      >  1:212832/1‑241 (MQ=255)                                                     tgattgttcttggtggtCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTgcgcgtacctaaatttaa                                                                                                                                                   >  1:137407/12‑238 (MQ=255)                                                     tgattgttcttggtggtCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTgcgcgtacc                                                                                                                                                            <  1:57031/231‑5 (MQ=255)                                                          gttcttggtggtCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTgcgcgtacctaaatttaatcgag                                                                                                                                              >  1:322230/7‑233 (MQ=255)                                                          gttcttggtggtCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTgcgcgtacctaaatttaatcgag                                                                                                                                              >  1:717953/7‑233 (MQ=255)                                                          gttcttggtggtCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTgcgcgtacctaaat                                                                                                                                                       <  1:275550/236‑10 (MQ=255)                                                            tcttggtggtCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTgcgcgtacctaaatttaatcgagaa                                                                                                                                            >  1:203027/5‑231 (MQ=255)                                                                    gtcTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTGCGCG‑TACCTAAATTTAATCGAGAAATGAAAAt                                                                                                                                   >  1:850041/3‑251 (MQ=255)                                                                              aaataaaaTAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTaaac                                                                                                                                                                                                       >  1:587545/8‑173 (MQ=255)                                                                              aaataaaaTAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTGCGCG‑TACCTAAATTTAATCGAGAAATGAAAATTTCCCGccc                                                                                                                          >  1:712035/8‑250 (MQ=255)                                                                                   aaaTAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTGCGCG‑TACCTAAATTTAATCGAGAAATGAAAATTTCCCGCCCCTGCaa                                                                                                                    <  1:689263/249‑1 (MQ=255)                                                                                                tgAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTAA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTGCGCG‑TACCTAAATTTAATCGAGAAATGAAAATTTCCCGCCCCTGCAAATACCCCTAAAGc                                                                                                       <  1:484113/250‑1 (MQ=255)                                                                                                tgAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTGGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTGCGCG‑TACCTAAATTTAATCGAGAAATGAAAATTTCCCGCCCCTGCAAATACCCCTAAAGc                                                                                                       <  1:575145/250‑1 (MQ=255)                                                                                                       ggTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTGCGCG‑TACCTAAATTTAATCGAGAAATGAAAATTTCCCGCCCCTGCAAATACCCCTAAAGCCACGtaa                                                                                                >  1:875811/1‑251 (MQ=255)                                                                                                           tttCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTGCGCG‑TACCTAAATTTAATCGAGAAATGAAAATTTCCCGCCCCTGCAAATACCCCTAAAGCCACGtaataa                                                                                             >  1:521567/1‑250 (MQ=255)                                                                                                           tttCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTGCGCG‑TACCTAAATTTAATCGAGAAATGAAAATTTCCCGCCCCTGCAAATACCCCTAAAGCCACGTAATAAc                                                                                            <  1:41444/251‑1 (MQ=255)                                                                                                              cATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTGCGCG‑TACCTAAATTTAATCGAGAAATGAAAATTTCCCGCCCCTGCAAATACCCCTAAAGCCACGTAATAActgc                                                                                         <  1:498703/251‑1 (MQ=255)                                                                                                                       cTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTGCGCG‑TACCTAAATTTAATCGAGAAATGAAAATTTCCCGCCCCTGCAAATACCCCTAAAGCCACGTAATAACTGCTGATTAGAt                                                                                <  1:790901/251‑1 (MQ=255)                                                                                                                               gtttgatagttttttaacgaaattGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTGCGCG‑TACCTAAATTTAATCGAGAAATGAAAATTTCCCGCCCCTGCAAATACCCCTAAAGCCACGTAATAACTGCTGATTAGATAATGCGAt                                                                        >  1:297496/24‑251 (MQ=255)                                                                                                                                          aaaaTCCAAAAATGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTGCGCG‑TACCTAAATTTAATCGAGAAATGAAAATTTCCCGCCCCTGCAAATACCCCTAAAGCCACGTAATAACTGCTGATTAGATAATGCGATACGTGCTTGaa                                                             <  1:341661/251‑1 (MQ=255)                                                                                                                                                      tGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTGCGCG‑TACCTAAATTTAATCGAGAAATGAAAATTTCCCGCCCCTGCAAATACCCCTAAAGCCACGTAATAAc                                                                                            >  1:400230/1‑208 (MQ=255)                                                                                                                                                         tACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTGCGCG‑TACCTAAATTTAATCGAGAAATGAAAATTTCCCGCCCCTGCAAATACCCCTAAAGCCACGTAATAACTGCTGATTAGATAATGc                                                                           <  1:531319/222‑1 (MQ=255)                                                                                                                                                         tACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTGCGCG‑TACCTAAATTTAATCGAGAAATGAAAATTTCCCGCCCCTGCAAATACCCCTAAAGCCACGTAATAACTGCTGATTAGATAATGCGATAc                                                                      >  1:303813/1‑227 (MQ=255)                                                                                                                                                         tACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTGCGCG‑TACCTAAATTTAATCGAGAAATGAAAATTTCCCGCCCCTGCAAATACCCCTAAAGCCACGTAATAACTGCTGATTAGATAATGCGATACg                                                                     <  1:613501/228‑1 (MQ=255)                                                                                                                                                          aCTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTGCGCG‑TACCTAAATTTAATCGAGAAATGAAAATTTCCCGCCCCTGCAAATACCCCTAAAGCCACGTAATAACTGCTGATTAGATAATGCGATACGTGCTTGAATTTATGCTTACAtttt                                             >  1:739068/1‑251 (MQ=255)                                                                                                                                                           cTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTGCGCG‑TACCTAAATTTAATCGAGAAATGAAAATTTCCCGCCCCTGCAAATACCCCTAAAGCCACGTAATAACTGCTGATTAGATAATGCGATACGTGCTTGAATTTATGCTTACAttttt                                            >  1:756241/1‑251 (MQ=255)                                                                                                                                                               gACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTgcgcgtacctaaatttaat                                                                                                                                                  >  1:89368/1‑132 (MQ=255)                                                                                                                                                               gACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTGCGCG‑TACCTAAATTTAATCGAGAAATGAAAATTTCCCGCCCCTGCAAATACCCCTaa                                                                                                          <  1:723725/185‑1 (MQ=255)                                                                                                                                                               gACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTGCGCG‑TACCTAAATTTAATCGAGAAATGAAAATTTCCCGCCCCTGCAAATACCCCTAAAGCCACGTAATAACTGCTGATTAGATAATGCGATACGTGCTTGAATTTa                                                         >  1:791023/1‑234 (MQ=255)                                                                                                                                                                 cAGATATAGTGATTTTCCTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCAGGCAGAATGTTATTCGGGCATTTTTATTTTTTCCGCG‑TCCATAAATTAAATCGAGAATTGAAAATTTCCCGCCCCTGCAAATACCCCTAAAGCCACGTAATAACTGCTGATTAGATAATGCGATAc                                                                      <  1:226758/219‑1 (MQ=255)                                                                                                                                                                    atatAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTGCGCG‑TACCTAAATTTAATCGAGAAATGAAAATTTCCCGCCCCTGCAAATACCCCTAAAGCCACGTAATAACTGCTGATTAGAt                                                                                <  1:171824/206‑1 (MQ=255)                                                                                                                                                                          tGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGGTTGCAGCCCGATAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTGCGCGTCCCA‑AAATTTAATCAAGAAATAAAAATTTACCGCCCCTGCAAATACCCCTAAAGCCACGTAATAACTGCTGATTAGATAATGCGATACGTGCTTGAATTTATGCTTACATTTTTAAATAAGTCTTGATa                             <  1:711642/251‑1 (MQ=255)                                                                                                                                                                           gATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTAttttt                                                                                                                                                                      <  1:524522/114‑3 (MQ=255)                                                                                                                                                                            aTTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTGCGCG‑TACCTAAATTTAATCGAGAAATGAAAATTTCCCGCCCCTGCAAATACCCCTAAAGCCACGTAATAAc                                                                                            >  1:799159/1‑186 (MQ=255)                                                                                                                                                                            aTTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTGCGCG‑TACCTAAATTTAATCGAGAAATGAAAATTTCCCGCCCCTGCAAATACCCCTAAAGCCACGTAATAACTGCTGATTAGATAATGCGATACGTGCTTGAATTTATGCTTACATTTTTAAATAAGTc                                   <  1:426022/243‑1 (MQ=255)                                                                                                                                                                            aTTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTGCGCG‑TACCTAAATTTAATCGAGAAATGAAAATTTCCCGCCCCTGCAAATACCCCTAAAGCCACGTAATAACTGCTGATTAGATAATGCGATACGTGCTTGAATTTATGCTTACATTTTTAAATAAGTCTTGATaaa                           <  1:268366/251‑1 (MQ=255)                                                                                                                                                                             ttttACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTGCGCG‑TACCTAAATTTAATCGAGAAATGAAAATTTCCCGCCCCTGCAAATACCCCTAAAGCCACGTAATAACTGCTGATTAGATAATGCGATACGTGCTTGAATTTATGCTTACATTTTTAAATAAGTCTTGATAAAt                          >  1:784907/1‑251 (MQ=255)                                                                                                                                                                                      aaGTGAAACTTGTCTAACTATTTTTGAGCAAGC‑TCAAAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTGCGCG‑TACCTAAATTTAATCGAGAAATGAAAATTTCCCGCCCCTGCAAATACCCCTAAAGCCACGTAATAACTGCTGATTAGATAATGCGATACGTGCTTGAATTTATGCTTACATTTTTAAATAAGTCTTGATAAATCAAtattta                 >  1:844796/1‑251 (MQ=255)                                                                                                                                                                                                      aactatttttgggcaagctcaaaaaTGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTGCGCG‑TACCTAAATTTAATCGAGAAATGAAAATTTCCCGCCCCTGCAAATACCCCTAAAGCCACGTAATAACTGCTGATTAGATAATGCGATACGTGCTTGAATTTATGCTTACATTTTTAAATAAGTCTTGATAAATCAATATTTATTGATGTTACAAgg   >  1:346280/22‑250 (MQ=255)                                                                                                                                                                                                      aactatttttgagcaagctcaaaaaTGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTGCGCG‑TACCTAAATTTAATCGAGAAATGAAAATTTCCCGCCCCTGCAAATACCCCTAAAGCCACGTAATAACTGCTGATTAGATAATGCGATACGTGCTTGAATTTATGCTTACATTTTTAAATAAGTCTTGATAAATCAATATTTATTGATGTTACAAGGc  <  1:236940/230‑1 (MQ=255)                                                                                                                                                                                                                      gctcaaaaaTGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTGCGCG‑TACCTAAATTTAATCGAGAAATGAAAATTTCCCGCCCCTGCAAATa                                                                                                                 <  1:720040/119‑1 (MQ=255)                                                                                                                                                                                                                         caaaaaTGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTGCGCG‑TACCTAAATTTAATCGAGAAATGAAAATTTCCCGCCCCTGCAAATACCCCTAAAg                                                                                                        <  1:233728/128‑1 (MQ=255)                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                 ACACTTATAAAACGCGACATTGTCACAGATGTAAGTGTAAAAAATGGAGATGTGATAGTTTTGGGTGGACTGGCCGAAAACAAGATAACTGAAGGCGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTA‑TTTTCCGCTTGC‑GG‑AAAATGCAGGGCGAAGGCTTGCAGCCCGGTAAATAATGCTCGATGTTATTCGGGCATTTTTATTTGTTGCGCG‑CTGATGATTTTAATCGAGAAATGAAAATTTCCCGCCCCTGCAAATACCCCTAAAGCCACGTAATAACTGCTGATTAGATAATGCGATACGTGCTTGAATTTATGCTTACATTTTTAAATAAGTCTTGATAAATCAATATTTATTGATGTTACAAGGC  >  1/1481345‑1481789

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑