Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,400,313	A→G	intergenic (+67695/+186820)	unknown → / ← unknown	tRNA‑Met‑CAT/tRNA‑Val‑GAC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,400,313	0	A	G	100.0%	254.2 / NA	76	intergenic (+67695/+186820)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Val‑GAC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (40/36);  total (40/36)

GGTGATAACCGCACATTGCAATACAACGAACCGCGTACCATGTTGCAGGATTTGAAGAAAATGGGTGTGCCGCAAGAGTTTATTTATCTGGATTTTGCGGGCTTTCGCACATTGGATTCGGTGATTCGTGCCGATCAGGTATTTAAAGCCCATTCCCTGACGATTATTTCGCAGAAATTTCATTGTGAACGCGCTTTATTCATCGCTAAATTCCACAATATTGACGCCATTTGTTTTGCAGCGGATTATCCGCAGGTGTATTCTTTCGTGCGTGTACGGGAATTTTTTGCCCGTTTGCAGGCTGTGTGGGATTTATTGGTAGAAAGGGAACCGCACTTTTTGGGGGCGCCGGAACCTTTGCCCCCGCCGGTGACGATTCCCGATGTTAACAGTGATATTTAATAATACTGCGGGTTAACGGCGTTGTATTGACGCTGTTTTTGTGTTTCTTCGACATGGCATCAAAGGTCAATG  >  1/1400074‑1400547                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                           ggTGATAACCGCACATTGCAATACAACGAACCGCGTACCATGTTGCAGGATTTGAAGAAAATGGGTGTGCCGCAAGAGTTTATTTATCTGGATTTTGCGGGCTTTCGCACATTGGATTCGGTGATTCGTGCCGATCAGGTATTTAAAGCCCATTCCCTGACGATTATTTCGCAGAAATTTCATTGTGAACGCGCTTTATTCATCGCTAAATTCCACAATATTGACGCCATTTGTTTTGCGGCGGATTATc                                                                                                                                                                                                                                  >  1:798566/1‑250 (MQ=255)  gTGATAACCGCACATTGCAATACAACGAACCGCGTACCATGTTGCAGGATTTGAAGAAAATGGGTGTGCCGCAAGAGTTTATTTATCTGGATTTTGCGGGCTTTCGCACATTGGATTCGGTGATTCGTGCCGATCAGGTATTTAAAGCCCATTCCCTGACGATTATTTCGCAGAAATTTCATTGTGAACGCGCTTTATTCATCGCTAAATTCCACAATATTGACGCCATTTGTTTTGCGGCGGATTATCCg                                                                                                                                                                                                                                >  1:125236/1‑251 (MQ=255)  gTGATAACCGCACATTGCAATACAACGAACCGCGTACCATGTTGCAGGATTTGAAGAAAATGGGTGTGCCGCAAGAGTTTATTTATCTGGATTTTGCGGGCTTTCGCACATTGGATTCGGTGATTCGTGCCGATCAGGTATTTAAAGCCCATTCCCTGACGATTATTTCGCAGAAATTTCATTGTGAACGCGCTTTATTCATCGCTAAATTCCACAATATTGACGCCATTTGTTTTGCGGCGGATTATCCg                                                                                                                                                                                                                                >  1:194368/1‑251 (MQ=255)      tAACCGCACATTGCAATACAACGAACCGCGTACCATGTTGCAGGATTTGAAGAAAATGGGTGTGCCGCAAGAGTTTATTTATCTGGATTTTGCGGGCTTTCGCACATTGGATTCGGTGATTCGTGCCGATCAGGAATTTAAAGCACTATCCCTGATGATTATTTCGCAGAAATTTCATTGTGAACGCGCTTTATTCATCGCTAAATTCCACAATATTGACGCCATTTGTTTTGCGGTGGATTATCCGCAgg                                                                                                                                                                                                                            >  1:93811/1‑251 (MQ=255)             acaTTGCAATACAACGAACCGCGTACCATGTTGCAGGATTTGAAGAAAATGGGTGTGCCGCAAGAGTTTATTTATCTGGATTTTGCGGGCTTTCGCACATTGGATTCGGTGATTCGTGCCGATCAGGTATTTAAAGCCCATTCCCTGACGATTATTTCGCAGAAATTTCATTGTGAACGCGCTTTATTCATCGCTAAATTCCACAATATTGACGCCATTTGTTTTGCGGCGGATTATCCGCAGGTGTATTc                                                                                                                                                                                                                     <  1:137303/251‑1 (MQ=255)             acaTTGCAATACAACGAACCGCGTACCATGTTGCAGGATTTGAAGAAAATGGGTGTGCCGCAAGAGTTTATTTATCTGGATTTTGCGGGCTTTCGCACATTGGATTCGGTGATTCGTGCCGATCAGGTATTTAAAGCCCATTCCCTGACGATTATTTCGCAGAAATTTCATTGTGAACGCGCTTTATTCATCGCTAAATTCCACAATATTGACGCCATTTGTTTTGCGGCGGATTATCCGCAGGTGTATTc                                                                                                                                                                                                                     <  1:635164/251‑1 (MQ=255)             acaTTGCAATACAACGAACCGCGTACCATGTTGCAGGATTTGAAGAAAATGGGTGTGCCGCAAGAGTTTATTTATCTGGATTTTGCGGGCTTTCGCACATTGGATTCGGTGATTCGTGCCGATCAGGTATTTAAAGCCCATTCCCTGACGATTATTTCGCAGAAATTTCATTGTGAACGCGCTTTATTCATCGCTAAATTCCACAATATTGACGCCATTTGTTTTGCGGCGGATTATCCGCAGGTGTATTc                                                                                                                                                                                                                     <  1:420452/251‑1 (MQ=255)                ttGCAATACAACGAACCGCGTACCATGTTGCAGGATTTGAAGAAAATGGGTGTGCCGCAAGAGTTTATTTATCTGGATTTTGCGGGCTTTCGCACATTGGATTCGGTGATTCGTGCCGATCAGGTATTTAAAGCCCATTCCCTGACGATTATTTCGCAGAAATTTCATTGTGAACGCGCTTTATTCATCGCTAAATTCCACAATATTGACGCCATTTGTTTTGCGGCGGATTATCCGCAGGTGTATTCttt                                                                                                                                                                                                                  >  1:412789/1‑251 (MQ=255)                ttGCAATACAACGAACCGCGTACCATGTTGCAGGATTTGAAGAAAATGGGTGTGCCGCAAGAGTTTATTTATCTGGATTTTGCGGGCTTTCGCACATTGGATTCGGTGATTCGTGCCGATCAGGTATTTAAAGCCCATTCCCTGACGATTATTTCGCAGAAATTTCATTGTGAACGCGCTTTATTCATCGCTAAATTCCACAATATTGACGCCATTTGTTTTGCGGCGGATTATCCGCAGGTGTATTCttt                                                                                                                                                                                                                  <  1:220991/251‑1 (MQ=255)                ttGCAATACAACGAACCGCGTACCATGTTGCAGGATTTGAAGAAAATGGGTGTGCCGCAAGAGTTTATTTATCTGGATTTTGCGGGCTTTCGCACATTGGATTCGGTGATTCGTGCCGATCAGGTATTTAAAGCCCATTCCCTGACGATTATTTCGCAGAAATTTCATTGTGAACGCGCTTTATTCATCGCTAAATTCCACAATATTGACGCCATTTGTTTTGCGGCGGATTATCCGCAGGTGTATTCttt                                                                                                                                                                                                                  >  1:149734/1‑251 (MQ=255)                  gCAATACAACGAACCGCGTACCATGTTGCAGGATTTGAAGAAAATGGGTGTGCCGCAAGAGTTTATTTATCTGGATTTTGCGGGCTTTCGCACATTGGATTCGGTGATTCGTGCCGATCAGGTATTTAAAGCCCATTCCCTGACGATTATTTCGCAGAAATTTCATTGTGAACGCGCTTTATTCATCGCTAAATTCCACAATATTGACGCCATTTGTTTTGCGGCGGATTATCCGCAGGTGTATTCTTTcg                                                                                                                                                                                                                >  1:213708/1‑251 (MQ=255)                     aTACAACGAACCGCGTACCATGTTGCAGGATTTGAAGAAAATGGGTGTGCCGCAAGAGTTTATTTATCTGGATTTTGCGGGCTTTCGCACATTGGATTCGGTGATTCGTGCCGATCAGGTATTTAAAGCCCATTCCCTGACGATTATTTCGCAGAAATTTCATTGTGAACGCGCTTTATTCATCGCTAAATTCCACAATATTGACGCCATTTGTTTTGCGGCGGATTATcc                                                                                                                                                                                                                                 <  1:875094/231‑1 (MQ=255)                       aCAACGAACCGCGTACCATGTTGCAGGATTTGAAGAAAATGGGTGTGCCGCAAGAGTTTATTTATCTGGATTTTGCGGGCTTTCGCACATTGGATTCGGTGATTCGTGCCGATCAGGTATTTAAAGCCCATTCCCTGACGATTATTTCGCAGAAATTTCATTGTGAACGCGCTTTATTCATCGCTAAATTCCACAATATTGACGCCATTTGTTTTGCGGCGGATTATCCGCAGGTGTATTCTTTcgtgcgt                                                                                                                                                                                                           >  1:211374/1‑251 (MQ=255)                                        aTGTTGCAGGATTTGAAGAAAATGGGTGTGCCGCAAGAGTTTATTTATCTGGATTTTGCGGGCTTTCGCACATTGGATTCGGTGATTCGTGCCGATCAGGTATTTAAAGCCCATTCCCTGACGATTATTTCGCAGAAATTTCATTGTGAACGCGCTTTATTCATCGCTAAATTCCACAATATTGACGCCATTTGTTTTGCGGCGGATTATCCGCAGGTGTATTCTTTCGTGCGTGTACGGGAATTTTTTg                                                                                                                                                                                           >  1:319641/1‑250 (MQ=255)                                          gTTGCAGGATTTGAAGAAAATGGGTGTGCCGCAAGAGTTTATTTATCTGGATTTTGCGGGCTTTCGCACATTGGATTCGGTGATTCGTGCCGATCAGGTATTTAAAGCCCATTCCCTGACGATTATTTCGCAGAAATTTCATTGTGAACGCGCTTTATTCATCGCTAAATTCCACAATATTGACGCCATTTGTTTTGCGGCGGATTATCCGCAGGTGCATTCTTTCGTGCGTGTACGGGAATTTTTTGcc                                                                                                                                                                                         >  1:714922/1‑250 (MQ=255)                                           ttGCAGGATTTGAAGAAAATGGGTGTGCCGCAAGAGTTTATTTATCTGGATTTTGCGGGCTTTCGCACATTGGATTCGGTGATTCGTGCCGATCAGGTATTTAAAGCCCATTCCCTGACGATTATTTCGCAGAAATTTCATTGTGAACGCGCTTTATTCATCGCTAAATTCCACAATATTGACGCCATTTGTTTTGCGGCGGATTATCCGCAGGTGTATTCTTTCGTGCGTGTACGGGAATTTTTTGcc                                                                                                                                                                                         <  1:855952/249‑1 (MQ=255)                                                ggATTTGAAGAAAATGGGTGTGCCGCAAGAGTTTATTTATCTGGATTTTGCGGGCTTTCGCACATTGGATTCGGTGATTCGTGCCGATCAGGTATTTAAAGCCCATTCCCTGACGATTATTTCGCAGAAATTTCATTGTGAACGCGCTTTATTCATCGCTAAATTCCACAATATTGACGCCATTTGTTTTGCGGCGGATTATCCGCAGGTGTATTCTTTcgtg                                                                                                                                                                                                              <  1:432075/223‑1 (MQ=255)                                                ggATTTGAAGAAAATGGGTGTGCCGCAAGAGTTTATTTATCTGGATTTTGCGGGCTTTCGCACATTGGATTCGGTGATTCGTGCCGATCAGGTATTTAAAGCCCATTCCCTGACGATTATTTCGCAGAAATTTCATTGTGAACGCGCTTTATTCATCGCTAAATTCCACAATATTGACGCCATTTGTTTTGCGGCGGATTATCCGCAGGTGTATTCTTTCGTGCGTGTACGGGAATTTTTTGCCCGTTTg                                                                                                                                                                                   >  1:747561/1‑250 (MQ=255)                                                    ttGAAGAAAATGGGTGTGCCGCAAGAGTTTATTTATCTGGATTTTGCGGGCTTTCGCACATTGGATTCGGTGATTCGTGCCGATCAGGTATTTAAAGCCCATTCCCTGACGATTATTTCGCAGAAATTTCATTGTGAACGCGCTTTATTCATCGCTAAATTCCACAATATTGACGCCATTTGTTTTGCGGCGGATTATCCGCAGGTGTATTCTTTCGTGCGTGTACGGGAATTTTTTGCCCGTTTGCAGGc                                                                                                                                                                              <  1:789893/251‑1 (MQ=255)                                                              tGGGTGTGCCGCAAGAGTTTATTTATCTGGATTTTGCGGGCTTTCGCACATTGGATTCGGTGATTCGTGCCGATCAGGTATTTAAAGCCCATTCCCTGACGATTATTTCGCAGAAATTTCATTGTGAACGCGCTTTATTCATCGCTAAATTCCACAATATTGACGCCATTTGTTTTGCGGCGGATTATCCGCAGGTGTATTCTTTCGTGCGTGTACGGGAATTTTTTGCCCGTTTGCAGGCTGTGTGGGAt                                                                                                                                                                    <  1:461397/251‑1 (MQ=255)                                                              tGGGTGTGCCGCAAGAGTTTATTTATCTGGATTTTGCGGGCTTTCGCACATTGGATTCGGTGATTCGTGCCGATCAGGTATTTAAAGCCCATTCCCTGACGATTATTTCGCAGAAATTTCATTGTGAACGCGCTTTATTCATCGCTAAATTCCACAATATTGACGCCATTTGTTTTGCGGCGGATTATCCGCAGGTGTATTCTTTCGTGCGTGTACGGGAATTTTTTGCCCGTTTGCAGGCTGTGTGGGAt                                                                                                                                                                    <  1:479108/251‑1 (MQ=255)                                                              tGGGTGTGCCGCAAGAGTTTATTTATCTGGATTTTGCGGGCTTTCGCACATTGGATTCGGTGATTCGTGCCGATCAGGTATTTAAAGCCCATTCCCTGACGATTATTTCGCAGAAATTTCATTGTGAACGCGCTTTATTCATCGCTAAATTCCACAATATTGACGCCATTTGTTTTGCGGCGGATTATCCGCAGGTGTATTCTTTCGTGCGTGTACGGGAATTTTTTGCCCGTTTGCAGGCTGTGTGGGAt                                                                                                                                                                    <  1:352786/251‑1 (MQ=255)                                                                         cAAGAGTTTATTTATCTGGATTTTGCGGGCTTTCGCACATTGGATTCGGTGATTCGTGCCGATCAGGTATTTAAAGCCCATTCCCTGACGATTATTTCGCAGAAATTTCATTGTGAACGCGCTTTATTCATCGCTAAATTCCACAATATTGACGCCATTTGTTTTGCGGCGGATTATCCGCAGGTGTATTCTTTCGTGCGTGTACGGGAATTTTTTGCCCGTTTGCAGGCTGTGTGGGATTTATTGGTAg                                                                                                                                                          <  1:813575/250‑1 (MQ=255)                                                                             agTTTATTTATCTGGATTTTGCGGGCTTTCGCACATTGGATTCGGTGATTCGTGCCGATCAGGTATTTAAAGCCCATTCCCTGACGATTATTTCGCAGAAATTTCATTGTGAACGCGCTTTATTCATCGCTAAATTCCACAATATTGACGCCATTTGTTTTGCGGCGGATTATCCGCAGGTGTATTCTTTCGTGCGTGTACGGGAATTTTTTGCCCGTTTGCAGGCTGTGTGGGAt                                                                                                                                                                    <  1:328905/236‑1 (MQ=255)                                                                              gTTTATTTATCTGGATTTTGCGGGCTTTCGCACATTGGATTCGGTGATTCGTGCCGATCAGGTATTTAAAGCCCATTCCCCGACGATTATTTCGCAGAAATTTCATTGTGAACGCGCTTTATTCATCGCTAAATTCCACAATATTGACGCCATTTGTTTTGCGGCGGATTATCCGCAGGTGTATTCTTTCGTGCGTGTACGGGAATTTTTTGCCCGTTTGCAGGCTGTGTGGGATTTATTGGTAGAAAgg                                                                                                                                                     <  1:455182/250‑1 (MQ=255)                                                                               tttatttaTCTGGATTTTGCGGGCTTTCGCACATTGGATTCGGTGATTCGTGCCGATCAGGTATTTAAAGCCCATTCCCTGACGATTATTTCGCAGAAATTTCATTGTGAACGCGCTTTATTCATCGCTAAATTCCACAATATTGACGCCATTTGTTTTGCGGCGGATTATCCGCAGGTGTATTCTTTCGTGCGTGTACGGGAATTTTTTGCCCGTTTGCAGGCTGTGTGGGATTTATTGGTAGAAAgg                                                                                                                                                     <  1:329707/249‑1 (MQ=255)                                                                               tttatttaTCTGGATTTTGCGGGCTTTCGCACATTGGATTCGGTGATTCGTGCCGATCAGGTATTTAAAGCCCATTCCCTGACGATTATTTCGCAGAAATTTCATTGTGAACGCGCTTTATTCATCGCTAAATTCCACAATATTGACGCCATTTGTGTTGCGGCGGATTATCCGCAGGTGTATTCTTTCGTGCGTGTACGGGAATTTTTTGCCCGTTTGCAGGCTGTGTGGGATTTATTGGTAGAAAgg                                                                                                                                                     <  1:875037/249‑1 (MQ=255)                                                                                ttatttatCTGGATTTTGCGGGCTTTCGCACATTGGATTCGGTGATTCGTGCCGATCAGGTATTTAAAGCCCATTCCCTGACGATTATTTCGCAGAAATTTCATTGTGAACGCGCTTTATTCATCGCTAAATTCCACAATATTGACGCCATTTGTTTTGCGGCGGATTATCCGCAGGTGTATTCTTTCGTGCGTGTACggg                                                                                                                                                                                                    >  1:34640/1‑201 (MQ=255)                                                                                ttatttatCTGGATTTTGCGGGCTTTCGCACATTGGATTCGGTGATTCGTGCCGATCAGGTATTTAAAGCCCATTCCCTGACGATTATTTCGCAGAAATTTCATTGTGAACGCGCTTTATTCATCGCTAAATTCCACAATATTGACGCCATTTGTTTTGCGGCGGATTATCCGCAGGTGTATTCTTTCGTGCGTGTACGGGAATTTTTTGCCCGTTTGCAGGCTGTGTGGGATTTATTGGTAGAAAGGGaa                                                                                                                                                  >  1:68803/1‑251 (MQ=255)                                                                                 tatttatCTGGATTTTGCGGGCTTTCGCACATTGGATTCGGTGATTCGTGCCGATCAGGTATTTAAAGCCCATTCCCTGACGATTATTTCGCAGAAATTTCATTGTGAACGCGCTTTATTCATCGCTAAATTCCACAATATTGACGCCATTTGTTTTGCGGCGGATTATCCGCAGGTGTATTCTTTCGTGCGTGTACGGGAATTTTTTGCCCGTTTGCAGGCTGTGTGGGATTTATTGGTAGAAAGGGaa                                                                                                                                                  >  1:594005/1‑250 (MQ=255)                                                                                     tatCTGGATTTTGCGGGCTTTCGCACATTGGATTCGGTGATTCGTGCCGATCAGGTATTTAAAGCCCATTCCCTGACGATTATTTCGCAGAAATTTCATTGTGAACGCGCTTTATTCATCGCTAAATTCCACAATATTGACGCCATTTGTTTTGCGGCGGATTATCCGCAGGTGTATTCTTTCGTGCGTGTACGGGAATTTTTTGCCCGTTTGCAGGCTGTGTGGGATTTATTg                                                                                                                                                              >  1:281966/1‑234 (MQ=255)                                                                                            aTTTTGCGGGCTTTCGCACATTGGATTCGGTGATTCGTGCCGATCAGGTATTTAAAGCCCATTCCCTGACGATTATTTCGCAGAAATTTCATTGTGAACGCGCTTTATTCATCGCTAAATTCCACAATATTGACGCCATTTGTTTTGCGGCGGATTATCCGCAGGTGTATTCTTTCGTGCGTGTACGGGAATTTTTTGCCCGTTTGCAGGCTGTGTGGGATTTATTGGTAGAAAGGGAACCGCACTTTTTg                                                                                                                                      <  1:262663/251‑1 (MQ=255)                                                                                                              caTTGGATTCGGTGATTCGTGCCGATCAGGTATTTAAAGCCCATTCCCTGACGATTATTTCGCAGAAATTTCATTGTGAACGCGCTTTATTCATCGCTAAATTCCACAATATTGACGCCATTTGTTTTGCGGCGGATTATCCGCAGGTGTATTc                                                                                                                                                                                                                     >  1:499344/1‑154 (MQ=255)                                                                                                              caTTGGATTCGGTGATTCGTGCCGATCAGGTATTTAAAGCCCATTCCCTGACGATTATTTCGCAGAAATTTCATTGTGAACGCGCTTTATTCATCGCTAAATTCCACAATATTGACGCCATTTGTTTTGCGGCGGATTATCCGCAGGTGTATTCTTTCGTGCGTGTACGGGAATTTTTTGCCCGTTTGCAGGCTGTGTGGGATTTATTGGTAGAAAGGGAACCGCACTTTTTGGGGGCGCCGGAACCTTTg                                                                                                                    >  1:728729/1‑251 (MQ=255)                                                                                                                  ggATTCGGTGATTCGTGCCGATCAGGTATTTAAAGCCCATTCCCTGACGATTATTTCGCAGAAATTTCATTGTGAACGCGCTTTATTCATCGCTAAATTCCACAATATTGACGCCATTTGTTTTGCGGCGGATTATCCGCAGGTGTATTCTTTCGTGCGTGTACGGGAATTTTTTGCCCGTTTGCAGGCTGTGTGGGATTTATTGGTAGAAAGGGAACCGCACTTTTTGGGGGCGCCGGAACCTTTGccc                                                                                                                 >  1:842408/1‑250 (MQ=255)                                                                                                                       cGGTGATTCGTGCCGATCAGGTATTTAAAGCCCATTCCCTGACGATTATTTCGCAGAAATTTCATTGTGAACGCGCTTTATTCATCGCTAAATTCCACAATATTGACGCCATTTGTTTTGCGGCGGATTATCCGCAGGTGTATTCTTTCGTGCGTGTACGGGAATTTTTTGCCCGTTTGCAGGc                                                                                                                                                                              <  1:31483/184‑1 (MQ=255)                                                                                                                                gTGCCGATCAGGTATTTAAAGCCCATTCCCTGACGATTATTTCGCAGAAATTTCATTGTGAACGCGCTTTATTCATCGCTAAATTCCACAATATTGACGCCATTTGTTTTGCGGCGGATTATCCGCAGGTGTATTCTTTCGTGCGTGTACGGGAATTTTTTGCCCGTTTGCAGGCTGTGTGGGATTTATTGGTAGAAAGGGAACCGCACTTTTTGGGGGCGCCGGAACCTTTGCCCCCGCCGGGGACGAtt                                                                                                  >  1:52267/1‑251 (MQ=255)                                                                                                                                     gATCAGGTATTTAAAGCCCATTCCCTGACGATTATTTCGCAGAAATTTCATTGTGAACGCGCTTTATTCATCGCTAAATTCCACAATATTGACGCCATTTGTTTTGCGGCGGATTATCCGCAGGTGTATTCTTTCGTGCGTGTACGGGAATTTTTTGCCCGTTTGCAGGCTGTGTGGGAt                                                                                                                                                                    <  1:24191/180‑1 (MQ=255)                                                                                                                                       tCAGGTATTTAAAGCCCATTCCCTGACGATTATTTCGCAGAAATTTCATTGTGAACGCGCTTTATTCATCGCTAAATTCCACAATATTGACGCCATTTGTTTTGCGGCGGATTATCCGCAGGTGTATTCTTTCGTGCGTGTACGGGAATTTTTTGCCCGTTTGCAGGCTGTGTGGGATTTATTGGTAGAAAGGGAACCGCACTTTTTGGGGGCGCCGGAACCTTTGCCCCCGCCGGTGACGATTCCCGATg                                                                                           <  1:878540/251‑1 (MQ=255)                                                                                                                                          ggTATTTAAAGCCCATTCCCTGACGATTATTTCGCAGAAATTTCATTGTGAACGCGCTTTATTCATCGCTAAATTCCACAATATTGACGCCATTTGTTTTGCGGCGGATTATCCGCAGGTGTATTCTTTCGTGCGTGTACGGGAATTTTTTGCCCGTTTGCAGGCTGTGTGGGATTTATTGGTAGAAAGGGAACCGCACTTTTTGGGGGCGCCGGAACCTTTGCCCCCGCCGGTGACGATTCCCGATGTTa                                                                                        >  1:7111/1‑251 (MQ=255)                                                                                                                                             aTTTAAAGCCCATTCCCTGACGATTATTTCGCAGAAATTTCATTGTGAACGCGCTTTATTCATCGCTAAATTCCACAATATTGACGCCATTTGTTTTGCGGCGGATTATCCGCAGGTGTATTCTTTCGTGCGTGTACGGGAATTTTTTGCCCGTTTGCAGGCTGTGTGGGATTTATTGGTAGAAAGGGAACCGCACTTTTTGGGGGCGCCGGAACCTTTGCCCCCGCCGGTGACGATTCCCGATGTTAACa                                                                                     >  1:769329/1‑251 (MQ=255)                                                                                                                                              tttAAAGCCCATTCCCTGACGATTATTTCGCAGAAATTTCATTGTGAACGCGCTTTATTCATCGCTAAATTCCACAATATTGACGCCATTTGTTTTGCGGCGGATTATCCGCGGGTGTATTCTTTCGTGCGTGTACGGGAATTTTTTGCCCGTTTGCAGGCTGTGTGGGATTTATTGGTAGAAAGGGAACCGCACTTTTTGGGGGCGCCGGAACCTTTGCCCCCGCCGGTGACGATTCCCGATGTTAACAg                                                                                    <  1:631678/251‑1 (MQ=255)                                                                                                                                              tttAAAGCCCATTCCCTGACGATTATTTCGCAGAAATTTCATTGTGAACGCGCTTTATTCATCGCTAAATTCCACAATATTGACGCCATTTGTTTTGCGGCGGATTATCCGCAGGTGTATTCTTTCGTGCGTGTACGGGAATTTTTTGCCCGTTTGCAGGCTGTGTGGGATTTATTGGTAGAAAGGGAACCGCACTTTTTGGGGGCGCCGGAACCTTTGCCCCCGCCGGTGACGATTCCCGATGTTAACAg                                                                                    <  1:196773/251‑1 (MQ=255)                                                                                                                                                    gCCCATTCCCTGACGATTATTTCGCAGAAATTTCATTGTGAACGCGCTTTATTCATCGCTAAATTCCACAATATTGACGCCATTTGTTTTGCGGCGGATTATCCGCAGGTGTATACTTTCGTGCGTGTACGGGAATTTTTTGCCCGTTTGCAGGc                                                                                                                                                                              >  1:388639/1‑155 (MQ=255)                                                                                                                                                         ttCCCTGACGATTATTTCGCAGAAATTTCATTGTGAACGCGCTTTATTCATCGCTAAATTCCACAATATTGACGCCATTTGTTTTGCGGCGGATTATCCGCAGGTGTATTCTTTCGTGCGTGTACGGAAATTTTTTGCCCGTTTGCAGGCTGTGTGGGATTTATTGGTAGAAAGGGAACCGCACTTTTTGGGGGCGCCGGAACCTTTGCCCCCGCCGGTGACGATTCCCGATGTTAACAGTGATATTtaat                                                                         <  1:578637/251‑1 (MQ=255)                                                                                                                                                          tCCCTGACGATTATTTCGCAGAAATTTCATTGTGAACGCGCTTTATTCATCGCTAAATTCCACAATATTGACGCCATTTGTTTTGCGGCGGATTATCCGCAGGTGTATTCTTTCGTGCGTGTACGGGAATTTTTTGCCCGTTTGCAGGCTGTGTGGGATTTATTGGTAGAAAGGGAACCGCACTTTTTGGGGGCGCCGGAACCTTTGCCCCCGCCGGTGACGATTCCCGATGTTAAc                                                                                      >  1:494896/1‑237 (MQ=255)                                                                                                                                                            ccTGACGATTATTTCGCAGAAATTTCATTGTGAACGCGCTTTATTCATCGCTAAATTCCACAATATTGACGCCATTTGTTTTGCGGCGGATTATCCGCAGGTGTATTCTTTCGTGCGTGTACGGGAATTTTTTGCCCGTTTGCAGGCTGTGTGGGATTTATTGGTAGAAAGGGAACCGCACTTTTTGGGGGCGCCGGAACCTTTGCCCCCGCCGGTGACGATTCCCGATGTTAACAGTGATATTTaataat                                                                      <  1:403653/251‑1 (MQ=255)                                                                                                                                                              tGACGATTATTTCTCAGAAATTTCATGGTGAACGGGCTTTATTCATGGCTAAATTCCACAAAATTGGCGCCGTTTGTTTTGCGGCGGATTATCGGCAGGTGTATTCTTTCGTGCGTGTACGGAAATTTTTTGCCCGTTTGCAGGCTGTGTGGGATTTATGGGTAGAAAGGGAACCGCTCTTTTTGGGGGCGCCGGAGCCTTTGCCCCCGCCGGTGACGATTCCCGATGTTAACAGTGATATTTAATAATAc                                                                    <  1:662179/251‑1 (MQ=255)                                                                                                                                                              tGACGATTATTTCGCAGAAATTTCATTGTGAACGCGCTTTATTCATCGCTAAATTCCACAATATTGACGCCTTTTGTTTTGCGGCGGATTATCCGCAGGTGTATTCTTTCGTGCGTGTACGGGAATTTTTTGCCCGTTTGCAGGCTGTGTGGGATTTATTGGTAGAAAGGGAACCGCACTTTTTGGGGGCGCCGGAACCTTTGCCCCCGCCGGTGACGATTCCCGATGTTAACAGTGATATTTAATAATAc                                                                    <  1:508380/251‑1 (MQ=255)                                                                                                                                                              tGACGATTATTTCGCAGAAATTTCATTGTGAACGCGCTTTATTCATCGCTAAATTCCACAATATTGACGCCATTTGTTTTGCGGCGGATTATCCGCAGGTGTATTCTTTCGTGCGTGTACGGGAATTTTTTGCCCGTTTGCAGGCTGTGTGGGATTTATTGGTAGAAAGGGAACCGCACTTTTTGGGGGCGCCGGAACCTTTGCCCCCGCCGGTGACGATTCCCGATGTTAACAGTGATATTTAATAATAc                                                                    <  1:740897/251‑1 (MQ=255)                                                                                                                                                              tGACGATTATTTCGCAGAAATTTCATTGTGAACGCGCTTTATTCATCGCTAAATTCCACAATATTGACGCCATTTGTTTTGCGGCGGATTATCCGCAGGTGTATTCTTTCGTGCGTGTACGGGAATTTTTTGCCCGTTTGCAGGCTGTGTGGGATTTATTGGTAGAAAGGGAACCGCACTTTTTGGGGGCGCCGGAACCTTTGCCCCCGCCGGTGACGATTCCCGATGTTAACAGTGATATTTAATAATAc                                                                    <  1:663784/251‑1 (MQ=255)                                                                                                                                                                 cGATTATTTCGCAGAAATTTCATTGTGAACGCGCTTTATTCATCGCTAAATTCCACAATATTGACGCCATTTGTTTTGCGGCGGATTATCCGCAGGTGTATTCTTTCGTGCGTGTACGGGAATTTTTTGCCCGTTTGCAGGCTGTGTGGGATTTATTGGTAGAAAGGGAACCGCACTTTTTGGGGGCGCCGGAACCTTTGCCCCCGCCGGTGACGATTCCCGATGTTAACAGTGATATTTAATAATACTg                                                                  >  1:623088/1‑250 (MQ=255)                                                                                                                                                                    ttattTCGCAGAAATTTCATTGTGAACGCGCTTTATTCATCGCTAAATTCCACAATATTGACGCCATTTGTTTTGCGGCGGATTATCCGCAGGTGTATTCTTTCGTGCGTGTACGGGAATTTTTTGCCCGTTTGCAGGCTGTGTGGGATTTATTGGTAGAAAGGGAACCGCACTTTTTGGGGGCGCCGGAACCTTTGCCCCCGCCGGTGACGATTCCCGATGTTAACAGTGATATTTAATAATACTGCggg                                                              >  1:569680/1‑251 (MQ=255)                                                                                                                                                                              gAAATTTCATTGTGAACGCGCTTTATTCATCGCTAAATTCCACAATATTGACGCCATTTGTTTTGCGGCGGATTATCCGCAGGTGTATTCTTTCGTGCGTGTACGGGAATTTTTTGCCCGTTTGCAGGCTGTGTGGGAt                                                                                                                                                                    >  1:248153/1‑139 (MQ=255)                                                                                                                                                                              gAAATTTCATTGTGAACGCGCTTTATTCATCGCTAAATTCCACAATATTGACGCCATTTGTTTTGCGGCGGATTATCCGCAGGTGTATTCTTTCGTGCGTGTACGGGAATTTTTTGCCCGTTTGCAGGCTGTGTGGGATTTATTGGTAGAAAGGGAACCGCACTTTTTGGGGGCGCCGGAACCTTTGCCCCCGCCGGTGACGATTCCCGATGTTAACAGTGATATTTAATAATAc                                                                    >  1:595528/1‑235 (MQ=255)                                                                                                                                                                               aaaTTTCATTGTGAACGCGCTTTATTCATCGCTAAATTCCACAATATTGACGCCATTTGTTTTGCGGCGGATTATCCGCAGGTGTATTCTTTCGTGCGTGTACGGGAATTTTTTGCCCGTTTGCAGGCTGTGTGGGATTTATTGGTAGAAAGGGAACCGCACTTTTTGGGGGCGCCGGAACCTTTGCCCCCGCCGGTGACGATTCCCGATGTTAACAGTGATATTTAATAATACTGCGGGTTAACGGCGtt                                                   >  1:522995/1‑251 (MQ=255)                                                                                                                                                                                 aTTTCATTGTGAACGCGCTTTATTCATCGCTAAATTCCACAATATTGACGCCATTTGTTTTGCGGCGGATTATCCGCAGGTGTATTCTTTCGTGCGTGTACGGGAATTTTTTGCCCGTTTGCAGGCTGTGTGGGATTTATTGGTAGAAAGGGAACCGCACTTTTTGGGGGCGCCGGAACCTTTGCCCCCGCCGGTGACGATTCCCGATGTTAACAGTGATAttt                                                                            <  1:480005/224‑1 (MQ=255)                                                                                                                                                                                       ttGTGAACGCGCTTTATTCATCGCTAAATTCCACAATATTGACGCCATTTGTTTTGCGGCGGATTATCCGCAGGTGTATTCTTTCGTGCGTGTACGGGAATTTTTTGCCCGTTTGCAGGCTGTGTGGGAtt                                                                                                                                                                   <  1:175327/131‑1 (MQ=255)                                                                                                                                                                                                cgcTTTATTCATCGCTAAATTCCACAATATGGACGCCATTTGTTTTGCGGCGGATTATCCGCAGGTGTATTCTTTCGTGCGTGTCCGGGAATTTTTTGCCCGTTTGCAGGCTGTGTGGGATTTATTGGTAGAAAGGGAACCGCACTTTTTGGGGGCGCCGGAACCTTTGCCCCCGCCGGTGACGATTCCCGATGTTAACAGTGATATTTAATAATAc                                                                    <  1:313959/217‑1 (MQ=255)                                                                                                                                                                                                 gcTTTATTCATCGCTAAATTCCACAATATTGACGCCATTTGTTTTGCGGCGGATTATCCGCAGGTGTATTCTTTCGTGCGTGTACGGGAATTTTTTGCCCGTTTGCAGGCTGTGTGGGATTTATTGGTAGAAAGGGAACCGCACTTTTTGGGGGCGCCGGAACCTTTGCCCCCGCCGGTGACGATTCCCGATGTTAACAGTGATATTtaat                                                                         <  1:380673/211‑1 (MQ=255)                                                                                                                                                                                                 gcTTTATTCATCGCTAAATTCCACAATATTGACGCCATTTGTTTTGCGGCGGATTATCCGCAGGTGTATTCTTTCGTGCGTGTACGGGAATTTTTTGCCCGTTTGCAGGCTGTGTGGGATTTATTGGTAGAAAGGGAACCGCACTTTTTGGGGGCGCCGGAACCTTTGCCCCCGCCGGTGACGATTCCCGATGTTAACAGTGATATTTAATAATACTGCGGGTTAACGGCGTTGTATTg                                             >  1:65543/1‑239 (MQ=255)                                                                                                                                                                                                  cTTTATTCATCGCTAAATTCCACAATATTGACGCCATTTGTTTTGCGGCGGATTATCCGCAGGTGTATTCTTTCGTGCGTGTACGGGAATTTTTTGCCCGTTTGCAGGCtgtgtg                                                                                                                                                                        <  1:365123/115‑1 (MQ=255)                                                                                                                                                                                                           tCGCTAAATTCCACAATATTGACGCCATTTGTTTTGCGGCGGATTATCCGCAGGTGTATTCTTTCGTGCGTGTACGGGAATTTTTTGCCCGTTTGCAGGCTGTGTGGGATTTATTGGTAGAAAGGGAACCGCACTTTTTGGGGGCGCCGGAACCTTTGCCCCCGCCGGTGACGATTCCCGATGTTAACAGTGATATTTAATAATACTGCGGGTTAACGGCGTTGTATTGACGCTGTTTTTGTGTTTCTTCg                       >  1:331490/1‑251 (MQ=255)                                                                                                                                                                                                                aaaTTCCACAATATTGACGCCATTTGTTTTGCGGCGGATTATCCGCAGGTGTATTCTTTCGTGCGTGTACGGGAATTTTTTGCCCGTTTGCAGGCTGTGTGGGATTTATTGGTAGAAAGGGAACCGCAc                                                                                                                                            >  1:644887/1‑129 (MQ=255)                                                                                                                                                                                                                  aTTCCACAATATTGACGCCATTTGTTTTGCGGCGGATTATCCGCAGGTGTATTCTTTCGTGCGTGTACGGGAATTTTTTGCCCGTTTGCAGGCTGTGTGGGATTTATTGGTAGAAAGGGAACCGCACTTTTTGGGGGCGCCGGAACCTTTGCCCCCGCCGGTGACGATTCCCGATGTTAACAGTGATATTTAATAATACTGCGGGTTAACGGCGTTGTATTGACGCTGTTTTTGTGTTTCTTCGACATgg                 >  1:639792/1‑250 (MQ=255)                                                                                                                                                                                                                   ttCCACAATATTGACGCCATTTGTTTTGCGGCGGATTATCCGCAGGTGTATTCTTTCGTGCGTGTACGGGAATTTTTTGCCCGTTTGCAGGCTGTGTGGGATTTATTGGTAGAAAGGGAACCGCACTTTTTGGGGGCGCCGGAACCTTTGCCCCCGCCGGTGACGATTCCCGATGTTAACAGTGATATTTAATAATAc                                                                    >  1:723910/1‑198 (MQ=255)                                                                                                                                                                                                                   ttCCACAATATTGACGCCATTTGTTTTGCGGCGGATTATCCGCAGGTGTATTCTTTCGTGCGTGTACGGGAATTTTTTGCCCGTTTGCAGGCTGTGTGGGATTTATTGGTAGAAAGGGAACCGCACTTTTTGGGGGCGCCGGAACCTTTGCCCCCGCCGGTGACGATTCCCGATGTTAACAGTGATATTTAATAATACTGCGGGTTAACGGCGTTGTATTGACGCTGTTTTTGTGTTTCTTCGACATGGCa               >  1:709356/1‑251 (MQ=255)                                                                                                                                                                                                                     ccACAATATTGACGCCATTTGTTTTGCGGCGGATTATCCGCAGGTGTATTCTTTCGTGCGTGTACGGGAATTTTTTGCCCGTTTGCAGGCTGTGTGGGATTTATTGGTAGAAAGGGAACCGCACTTTTTGGGGGCGCCGGAACCTTTGCCCCCGCCGGTGACGATTCCCAATGTTAACAGTGATATTTAATAATAc                                                                    >  1:435190/1‑196 (MQ=255)                                                                                                                                                                                                                        caATATTGACGCCATTTGTTTTGCGGCGGATTATCCGCAGGTGTATTCTTTCGTGCGTGTACGGGAATTTTTTGCCCGTTTGCAGGCTGTGTGGGATTTATTGGTAGAAAGGGAACCGCACTTTTTGGGGGCGCCGGAACCTTTGCCCCCGCCGGTGACGATTCCCGATGTTAACAGTGATATTTAATAATACTGCGGGTTAACGGCGTTGTATTGACGCTGTTTTTGTGTTTCTTCGACATGGCATCaa           >  1:45663/1‑250 (MQ=255)                                                                                                                                                                                                                          atatTGACGCCATTTGTTTTGCGGCGGATTATCCGCAGGTGTATTCTTTCGTGCGTGTACGGGAATTTTTTGCCCGTTTGCAGGCTGTGTGGGATTTATTGGTAGAAAGGGAACCGCACTTTTTGGGGGCGCCGGAACCTTTGCCCCCGCCGGTGACGATTCCCGATGTTAACAGTGATATTTAATAATACTGCGGGTTAACGGCGTTGTATTGACGCTGTTTTTGTGTTTCTTCGACATGGCATCAAAgg        >  1:19336/1‑251 (MQ=255)                                                                                                                                                                                                                          atatTGACGCCATTTGTTTTGCGGCGGATTATCCGCAGGTGTATTCTTTCGTGCGTGTACGGGAATTTTTTGCCCGTTTGCAGGCTGTGTGGGATTTATTGGTAGAAAGGGAACCGCACTTTTTGGGGGCGCCGGAACCTTTGCCCCCGCCGGTGACGATTCCCGATGTTAACAGTGATATTTAATAATACTGCGGGTTAACGGCGTTGTATTGACGCTGTTTTTGTGTTTCTTCGACATGGCATCAAAgg        >  1:512333/1‑251 (MQ=255)                                                                                                                                                                                                                          atatTGACGCCATTTGTTTTGCGGCGGATTATCCGCAGGTGTATTCTTTCGTGCGTGTACGGGAATTTTTTGCCCGTTTGCAGGCTGTGTGGGATTTATTGGTAGAAAGGGAACCGCACTTTTTGGGGGCGCCGGAACCTTTGCCCCCGCCGGTGACGATTCCCGATGTTAACAGTGATATTTAATAATACTGCGGGTTAACGGCGTTGTATTGACGCTGTTTTTGTGTTTCTTCGACATGGCATCAAAgg        <  1:144588/251‑1 (MQ=255)                                                                                                                                                                                                                            atTGACGCCATTTGTTTTGCGGCGGATTATCCGCAGGTGTATTCTTTCGTGCGTGTACGGGAATTTTTTGCCCGTTTGCAGGCTGTGTGGGATTTATTGGTAGAAAGGGAACCGCACTTTTTGGGGGCGCCGGAACCTTTGCCCCCGCCGGTGACGATTCCCGATGTTAACAGTGATATTTAATAATACTGCGGGTTAACGGCGTTGTATTGACGCTGTTTTTGTGTTTCTTCGACATGGCATCAAAGGt       >  1:187985/1‑250 (MQ=255)                                                                                                                                                                                                                                aCGCCTTTTGTTTTGCGGCGGATTATCCGCAGGTTTATTCTTTGTTGCGTGTACGGGAATTTTTGGCCCGTTTGCAGGCTGTGTGGGATTTATTGGTAGAAAGGAAACCGCACTTTTTGGGGGGGCGGGACCCTTTGCCCCCGCCGGTGACGATTCCCGATGTTAACAGTGATATTTAATAATACTGCGGGTTAACGGCGTTGTATTGACGCTGTTTTTGTGTTTCTTCGACATGGCATCAAAGGTCAATg  <  1:879668/251‑1 (MQ=255)                                                                                                                                                                                                                                   ccATTTGTTTTGCGGCGGATTATCCGCAGGGGTATTCTTTCGTGCGTGTACGGGAATTTTTTGCCCGTTTGCAGGCTGTGTGGGATTTATTGGTAGAAAGGGAAc                                                                                                                                                 >  1:409346/1‑105 (MQ=255)                                                                                                                                                                                                                                    cATTTGTTTTGCGGCGGATTATCCGCAGGTGTATTCTTTCGTGCGTGTACGGGAATTTTTTGCCCGTTTGCAGGCTGTGTGGGATTTATTGGTAGAAAGGGAACCGCACTTTTTGGGGGCGCCGGAACCTTTGCCCCCGCCGGTGACGATTCCCGATGTTAACAg                                                                                    <  1:132491/165‑1 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                           GGTGATAACCGCACATTGCAATACAACGAACCGCGTACCATGTTGCAGGATTTGAAGAAAATGGGTGTGCCGCAAGAGTTTATTTATCTGGATTTTGCGGGCTTTCGCACATTGGATTCGGTGATTCGTGCCGATCAGGTATTTAAAGCCCATTCCCTGACGATTATTTCGCAGAAATTTCATTGTGAACGCGCTTTATTCATCGCTAAATTCCACAATATTGACGCCATTTGTTTTGCAGCGGATTATCCGCAGGTGTATTCTTTCGTGCGTGTACGGGAATTTTTTGCCCGTTTGCAGGCTGTGTGGGATTTATTGGTAGAAAGGGAACCGCACTTTTTGGGGGCGCCGGAACCTTTGCCCCCGCCGGTGACGATTCCCGATGTTAACAGTGATATTTAATAATACTGCGGGTTAACGGCGTTGTATTGACGCTGTTTTTGTGTTTCTTCGACATGGCATCAAAGGTCAATG  >  1/1400074‑1400547

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑