Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,223,066	T→C	intergenic (‑112684/‑83499)	unknown ← / → unknown	tRNA‑Gly‑GCC/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,223,066	0	T	C	100.0%	235.5 / NA	70	intergenic (‑112684/‑83499)	unknown/unknown	tRNA‑Gly‑GCC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (44/26);  total (44/26)

GCTCGGCTTAGAAGCGCTGAATTTTGCTAACGAAGGCAAACTGGTCATCATCGCCGCCCCTGAAAAAACACCTGAAATTCTGACCGCACTTCACTCGCACGAACTGGGAAAAAATGCCGCAGTCATCGGCGAAGTGACGACGGATAAAAAAGTGAGATTGTTGGGCAGCTTCGGGCAGTCACGGCTGTTGGATTTGCCGGTGAATGAGCCGTTACCAAGGATTTGTTGATTTTTTATCGGGTTTTGCCCGATGGGCGCCTTACTTTCTTTTACTTGCATAAAAGAAAGTAAGCAAAGAAAAGTGCACCCCAGCTTCACCGCTTATCCTCGCTCACTTGCAATTTTCTTAACGAAAATTTTCGAACTCGCCATTTCATGGCTCAAACAGGCGAAAATTTCCTAAAAATTGCAAATCGCTCAGGCGGCTCAGATGGGGGCCCCCTGTTTTGTCGGAGCATCAAAAAAGTGCGGTACATTTTGAAAT  >  1/1222822‑1223305                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                gCTCGGCTTAGAAGCGCTGAATTTTGCTAACGAAGGCAAACTGGTCATCATCGCCGCCCCTGAAAAAACACCTGAAATTCTGACCGCACTTCACTCGCACGAACTGGGAAAAAATGCCGCAGTCATCGGCGAAGTGACGACGGATAAAAAAGTGAGATTGTTGGGCAGCTTCGGGCAGTCACGGCTGTTGGATTTGCCGGTGAATGAGCCGTTACCAAGGATTTGTTGATTTTTTATCGGGTTTCGCCCGa                                                                                                                                                                                                                                           >  1:385062/1‑251 (MQ=255)      gCTTAGAAGCGCTGAATTTTGCTAACGAAGGCAAACTGGTCATCATCGCCGCCCCTGAAAAAACACCTGAAATTCTGACCGCACTTCACTCGCACGAACTGGGAAAAAATGCCGCAGTCATCGGCGAAGTGACGACGGATAAAAAAGTGAGATTGTTGGGCAGCTTCGGGCAGTCACGGCTGTTGGATTTGCCGGTGAATGAGCCGTTACCAAGGATTTGTTGATTTTTTATCGGGTTTCGCCCGATgggg                                                                                                                                                                                                                                      >  1:844719/1‑250 (MQ=255)      gCTTAGAAGCGCTGAATTTTGCTAACGAAGGCAAACTGGTCATCATCGCCGCCCCTGAAAAAACACCTGAAATTCTGACCGCACTTCACTCGCACGAACTGGGAAAAAATGCCGCAGTCATCGGCGAAGTGACGACGGATAAAAAAGTGAGATTGTTGGGCAGCTTCGGGCAGTCACGGCTGTTGGATTTGCCGGTGAATGAGCCGTTACCAAGGATTTGTTGATTTTTTATCGGGTTTCGCCCGATggg                                                                                                                                                                                                                                       >  1:845404/1‑250 (MQ=255)      gCTTAGAAGCGCTGAATTTTGCTAACGAAGGCAAACTGGTCATCATCGCCGCCCCTGAAAAAACACCTGAAATTCTGACCGCACTTCACTCGCACGAACTGGGAAAAAATGCCGCAGTCATCGGCGAAGTGACGACGGATAAAAAAGTGAGATTGTTGGGCAGCTTCGGGCAGTCACGGCTGTTGGATTTGCCGGTGAATGAGCCGTTACCAAGGATTTGTTGATTTTTTATCGGGTTTCGCCCGATggg                                                                                                                                                                                                                                       >  1:724793/1‑250 (MQ=255)      gCTTAGAAGCGCTGAATTTTGCTAACGAAGGCAAACTGGTCATCATCGCCGCCCCTGAAAAAACACCTGAAATTCTGACCGCACTTCACTCGCACGAACTGGGAAAAAATGCCGCAGTCATCGGCGAAGTGACGACGGATAAAAAAGTGAGATTGTTGGGCAGCTTCGGGCAGTCACGGCTGTTGGATTTGCCGGTGAATGAGCCGTTACCAAGGATTTGTTGATTTTTTATCGGGTTTCGCCCGATggg                                                                                                                                                                                                                                       >  1:569491/1‑250 (MQ=255)      gCTTAGAAGCGCTGAATTTTGCTAACGAAGGCAAACTGGTCATCATCGCCGCCCCTGAAAAAACACCTGAAATTCTGACCGCACTTCACTCGCACGAACTGGGAAAAAATGCCGCAGTCATCGGCGAAGTGACGACGGATAAAAAAGTGAGATTGTTGGGCAGCTTCGGGCAGTCACGGCTGTTGGATTTGCCGGTGAATGAGCCGTTACCAAGGATTTGTTGATTTTTTATCGGGTTTCGCCCGATggg                                                                                                                                                                                                                                       >  1:523981/1‑250 (MQ=255)      gCTTAGAAGCGCTGAATTTTGCTAACGAAGGCAAACTGGTCATCATCGCCGCCCCTGAAAAAACACCTGAAATTCTGACCGCACTTCACTCGCACGAACTGGGAAAAAATGCCGCAGTCATCGGCGAAGTGACGACGGATAAAAAAGTGAGATTGTTGGGCAGCTTCGGGCAGTCACGGCTGTTGGATTTGCCGGTGAATGAGCCGTTACCAAGGATTTGTTGATTTTTTATCGGGTTTCGCCCGATggg                                                                                                                                                                                                                                       >  1:381796/1‑250 (MQ=255)      gCTTAGAAGCGCTGAATTTTGCTAACGAAGGCAAACTGGTCATCATCGCCGCCCCTGAAAAAACACCTGAAATTCTGACCGCACTTCACTCGCACGAACTGGGAAAAAATGCCGCAGTCATCGGCGAAGTGACGACGGATAAAAAAGTGAGATTGTTGGGCAGCTTCGGGCAGTCACGGCTGTTGGATTTGCCGGTGAATGAGCCGTTACCAAGGATTTGTTGATTTTTTATCGGGTTTCGCCCGATggg                                                                                                                                                                                                                                       >  1:361776/1‑250 (MQ=255)      gCTTAGAAGCGCTGAATTTTGCTAACGAAGGCAAACTGGTCATCATCGCCGCCCCTGAAAAAACACCTGAAATTCTGACCGCACTTCACTCGCACGAACTGGGAAAAAATGCCGCAGTCATCGGCGAAGTGACGACGGATAAAAAAGTGAGATTGTTGGGCAGCTTCGGGCAGTCACGGCTGTTGGATTTGCCGGTGAATGAGCCGTTACCAAGGATTTGTTGATTTTTTATCGGGCTTCGCCCGATggg                                                                                                                                                                                                                                       >  1:774460/1‑250 (MQ=255)      gCTTAGAAGCGCTGAATTTTGCTAACGAAGGCAAACTGGTCATCATCGCCGCCCCTGAAAAAACACCTGAAATTCTGACCGCACTTCACTCGCACGAACTGGGAAAAAATGCCGCAGTCATCGGCGAAGTGACGACGGATAAAAAAGTGAGATTGTTGGGCAGCTTCGGACAGTCACGGCTGTTGGATTTGCCGGTGAATGAGCCGTTACCAAGGATTTGTTGATTTTTTATCGGGTTTCGCCCGATggg                                                                                                                                                                                                                                       >  1:439216/1‑250 (MQ=255)      gCTTAGAAGCGCTGAATTTTGCTAACGAAGGCAAACTGGTCATCATCGCCGCCCCTGAAAAAACACCTGAAATTCTGACCGCACTTCACTCGCACGAACTGGGAAAAAATGCCGCAGTCATCGGCGAAGTGACGACGGATAAAAAAGTGAGATTGTTGGGCAGCTTCGGACAGTCACGGCTGTTGGATTTGCCGGTGAATGAGCCGTTACCAAGGATTTGTTGATTTTTTATCGGGTTTCGCCCGATggg                                                                                                                                                                                                                                       >  1:428214/1‑250 (MQ=255)        ttAGAAGCGCTGAATTTTGCTAACGAAGGCAAACTGGTCATCATCGCCGCCCCTGAAAAAACACCTGAAATTCTGACCGCACTTCACTCGCACGAACTGGGAAAAAATGCCGCAGTCATCGGCGAAGTGACGACGGATAAAAAAGTGAGATTGTTGGGCAGCTTCGGGCAGTCACGGCTGTTGGATTTGCCGGTGAATGAGCCGTTACCAAGGATTTGTTGATTTTTTATCGGGTTTCGCCCGATGGgcgc                                                                                                                                                                                                                                    <  1:155034/251‑1 (MQ=255)                 cTGAATTTTGCTAACGAAGGCAAACTGGTCATCATCGCCGCCCCTGAAAAAACACCTGAAATTCTGACCGCACTTCACTCGCACGAACTGGGAAAAAATGCCGCAGTCATCGGCGAAGTGACGACGGATAAAAAAGTGAGATTGTTGGGCAGCTTCGGGCAGTCACGGCTGTTGGATTTGCCGGTGAATGAGCCGTTACCAAGGATTTGTTGATTTTTTATCGGGTTTCGCCCGATGGGCGCCTTActtt                                                                                                                                                                                                                            <  1:201210/250‑1 (MQ=255)                    aaTTTTGCTAACGAAGGCAAACTGGTCATCATCGCCGCCCCTGAAAAAACACCTGAAATTCTGACCGCACTTCACTCGCACGAACTGGGAAAAAATGCCGCAGTCATCGGCGAAGTGACGACGGATAAAAAAGTGAGATTGTTGGGCAGCTTCGGGCAGTCACGGCTGTTGGATTTGCCGGTGAATGAGCCGTTACCAAGGATTTGTTGATTTTTTATCGGGTTTCGCCCGATGGGCGCCTTActttcttt                                                                                                                                                                                                                        <  1:191365/251‑1 (MQ=255)                        ttGCTAACGAAGGCAAACTGGTCATCATCGCCGCCCCTGAAAAAACACCTGAAATTCTGACCGCACTTCACTCGCACGAACTGGGAAAAAATGCCGCAGTCATCGGCGAAGTGACGACGGATAAAAAAGTGAGATTGTTGGGCAGCTTCGGGCAGTCACGGCTGTTGGATTTGCCGGTGAATGAGCCGTTACCAAGGATTTGTTGATTTTTTATCGGGTTTCGCCCGATGGGCGCCTTACTTTCTTTTa                                                                                                                                                                                                                      >  1:273538/1‑249 (MQ=255)                                    gCAAACTGGTCATCATCGCCGCCCCTGAAAAAACACCTGAAATTCTGACCGCACTTCACTCGCACGAACTGGGAAAAAATGCCGCAGTCATCGGCGAAGTGACGACGGATAAAAAAGTGAGATTGTTGGGCAGCTTCGGGCAGTCACGGCTGTTGGATTTGCCGGTGAATGAGCCGTTACCAAGGATTTGTTGATTTTTTATCGGGTTTCGCCCGATGGGCGCCTTACTTTCTTTTACTTGCATAaaag                                                                                                                                                                                                          <  1:194632/249‑1 (MQ=255)                                       aaCTGGTCATCATCGCCGCCCCTGAAAAAACACCTGAAATTCTGACCGCACTTCACTCGCACGAACTGGGAAAAAATGCCGCAGTCATCGGCGAAGTGACGACGGATAAAAAAGTGAGATTGTTGGGCAGCTTCGGGCAGTCACGGCTGTTGGATTTGCCGGTGAATGAGCCGTTACCAAGGATTTGTTGATTTTTTATCGGGTTTCGCCCGATGGGCGCCTTACTTTCTTTTACTTGCATAAAAGAAAGt                                                                                                                                                                                                     >  1:514597/1‑251 (MQ=255)                                         cTGGTCATCATCGCCGCCCCTGAAAAAACACCTGAAATTCTGACCGCACTTCACTCGCACGAACTGGGAAAAAATGCCGCAGTCATCGGCGAAGTGACGACGGATAAAAAAGTGAGATTGTTGGGCAGCTTCGGGCAGTCACGGCTGTTGGATTTGCCGGTGAATGAGCCGTTACCAAGGATTTGTTGATTTTTTATCGGGTTTCGCCCGATGGGCGCCTTACTTTCTTTTACTTGCATAAAAGAAAGTaa                                                                                                                                                                                                   >  1:514266/1‑251 (MQ=255)                                           ggTCATCATCGCCGCCCCTGAAAAAACACCTGAAATTCTGACCGCACTTCACTCGCACGAACTGGGAAAAAATGCCGCAGTCATCGGCGAAGTGACGACGGATAAAAAAGTGAGATTGTTGGGCAGCTTCGGGCAGTCACGGCTGTTGGATTTGCCGGTGAATGAGCCGTTACCAAGGATTTGTTGATTTTTTATCGGGTTTCGCCCGATGGGCGCCTTACTTTCTTTTACTTGCATAAAAGAAAGTAAg                                                                                                                                                                                                  >  1:852579/1‑250 (MQ=255)                                            gTCATCATCGCCGCCCCTGAAAAAACACCTGAAATTCTGACCGCACTTCACTCGCACGAACTGGGAAAAAATGCCGCAGTCATCGGCGAAGTGACGACGGATAAAAAAGTGAGATTGTTGGGCAGCTTCGGGCAGTCACGGCTGTTGGATTTGCCGGTGAATGAGCCGTTACCAAGGATTTGTTGATTTTTTATCGGGTTTCGCCCGATGGGCGCCTTACTTTCTTTTACTTGCATAAAAGAAAGTAAGc                                                                                                                                                                                                 <  1:307184/250‑1 (MQ=255)                                              catcatCGCCGCCCCTGAAAAAACACCTGAAATTCTGACCGCACTTCACTCGCACGAACTGGGAAAAAATGCCGCAGTCATCGGCGAAGTGACGACGGATAAAAAAGTGAGATTGTTGGGCAGCTTCGGGCAGTCACGGCTGTTGGATTTGCCGGTGAATGAGCCGTTACCAAGGATTTGTTGATTTTTTATCGGGTTTCGCCCGATGGGCGCCTTActtt                                                                                                                                                                                                                            >  1:713392/1‑221 (MQ=255)                                                  atcGCCGCCCCTGAAAAAACACCTGAAATTCTGACCGCACTTCACTCGCACGAACTGGGAAAAAATGCCGCAGTCATCGGCGAAGTGACGACGGATAAAAAAGTGAGATTGTTGGGCAGCTTCGGGCAGTCACGGCTGTTGGATTTGCCGGTGAATGAGCCGTTACCAAGGATTTGTTGATTTTTTATCGGGTTTCGCCCGATGGGCGCCTTACTTTCTTTTACTTGCATAAAAGAAAGTAAGCAAAGaaa                                                                                                                                                                                          <  1:377945/251‑1 (MQ=255)                                                  atcGCCGCCCCTGAAAAAACACCTGAAATTCTGACCGCACTTCACTCGCACGAACTGGGAAAAAATGCCGCAGTCATCGGCGAAGTGACGACGGATAAAAAAGTGAGATTGTTGGGCAGCTTCGGGCAGTCACGGCTGTTGGATTTGCCGGTGAATGAGCCGTTACCAAGGATTTGTTGATTTTTTATCGGGTTTCGCCCGATGGGCGCCTTACTTTCTTTTACTTGCATAAAAGAAAGTAAGCAAAGaaa                                                                                                                                                                                          <  1:402713/251‑1 (MQ=255)                                                  atcGCCGCCCCTGAAAAAACACCTGAAATTCTGACCGCACTTCACTCGCACGAACTGGGAAAAAATGCCGCAGTCATCGGCGAAGTGACGACGGATAAAAAAGTGAGATTGTTGGGCAGCTTCGGGCAGTCACGGCTGTTGGATTTGCCGGTGAATGAGCCGTTACCAAGGATTTGTTGATTTTTTATCGGGTTTCGCCCGATGGGCGCCTTACTTTCTTTTACTTGCATAAAAGAAAGTAAGCAAAGaaa                                                                                                                                                                                          <  1:370150/251‑1 (MQ=255)                                                   tcGCCGCCCCTGAAAAAACACCTGAAATTCTGACCGCACTTCACTCGCACGAACTGGGAAAAAATGCCGCAGTCATCGGCGAAGTGACGACGGATAAAAAAGTGAGATTGTTGGGCAGCTTCGGGCAGTCACGGCTGTTGGATTTGCCGGTGAATGAGCCGTTACCAAGGATTTGTTGATTTTTTATCGGGTTTCGCCCGATGGGCGCCTTACTTTCTTTTACTTGCATAAAAGAAAGTAAGCAAAGaaaa                                                                                                                                                                                         >  1:725395/1‑251 (MQ=255)                                                       cgccCCTGAAAAAACACCTGAAATTCTGACCGCACTTCACTCGCACGAACTGGGAAAAAATGCCGCAGTCATCGGCGAAGTGACGACGGATAAAAAAGTGAGATTGTTGGGCAGCTTCGGGCAGTCACGGCTGTTGGATTTGCCGGTGAATGAGCCGTTACCAAGGATTTGTTGATTTTTTATCGGGTTTCGCCCGATGGGCGCCTTACTTTCTTTTACTTGCATAAAAGAAAGTAAGCAAAGAAAAGTGc                                                                                                                                                                                     <  1:519972/251‑1 (MQ=255)                                                                  aaaCACCTGAAATTCTGACCGCACTTCACTCGCACGAACTGGGAAAAAATGCCGCAGTCATCGGCGAAGTGACGACGGATAAAAAAGTGAGATTGTTGGGCAGCTTCGGGCAGTCACGGCTGTTGGATTTGCCGGTGAATGAGCCGTTACCAAGGATTTGTTGATTTTTTATCGGGTTTCGCCCGATGGGCGCCTTACTTTCTTTTACTTGCATAAAAGAAAGTAAGCAAAGAAAAGTGCACCCCAGCTTc                                                                                                                                                                          <  1:209354/251‑1 (MQ=255)                                                                       ccTGAAATTCTGACCGCACTTCACTCGCACGAACTGGGAAAAAATGCCGCAGTCATCGGCGAAGTGACGACGGATAAAAAAGTGAGATTGTTGGGCAGCTTCGGGCAGTCACGGCTGTTGGATTTGCCGGTGAATGAGCCGTTACCAAGGATTTGTTGATTTTTTATCGGGTTTCGCCCGATGGGCGCCTTACTTTCTTTTAc                                                                                                                                                                                                                     >  1:183627/1‑203 (MQ=255)                                                                       ccTGAAATTCTGACCGCACTTCACTCGCACGAACTGGGAAAAAATGCCGCAGTCATCGGCGAAGTGACGACGGATAAAAAAGCGAGATTGTTGGGCAGCTTCGGGCAGTCACGGCTGTTGGATTTGCCGGTGAATGAGCCGTTACCAAGGATTTGTTGATTTTTTATCGGGTTTCGCCCGATGGGCGCCTTACTTTCTTTTACTTGCATAAAAGAAAGTAAGCAAAGAAAAGTGCACCCCAGCTTCACCg                                                                                                                                                                      >  1:798071/1‑250 (MQ=255)                                                                            aaTTCTGACCGCACTTCACTCGCACGAACTGGGAAAAAATGCCGCAGTCATCGGCGAAGTGACGACGGATAAAAAAGTGAGATTGTTGGGCAGCTTCGGGCAGTCACGGCTGTTGGATTTGCCGGTGAATGAGCCGTTACCAAGGATTTGTTGATTTTTTATCGGGTTTCGCCCGATGGGCGCCTTACTTTCTTTTACTTGCATAaaag                                                                                                                                                                                                          >  1:411414/1‑209 (MQ=255)                                                                             aTTCTGACCGCACTTCACTCGCACGAACTGGGAAAAAATGCCGCAGTCATCGGCGAAGTGACGACGGATAAAAAAGTGAGATTGTTGGGCAGCTTCGGGCAGTCACGGCTGTTGGATTTGCCGGTGAATGAGCCGTTACCAAGGATTTGTTGATTTTTTATCGGGTTTCGCCCGATGGGCGCCTTACTTTCTTTTACTTGCATAAAAGAAAGTAAGCAAAGAAAAGTGCACCCCAGCTTCACCGCTTATc                                                                                                                                                                >  1:801556/1‑250 (MQ=255)                                                                                   aCCGCACTTCACTCGCACGAACTGGGAAAAAATGCCGCAGTCATCGGCGAAGTGACGACGGATAAAAAAGTGAGATTGTTGGGCAGCTTCGGGCAGTCACGGCTGTTGGATTTGCCGGTGAATGAGCCGTTACCAAGGATTTGTTGATTTTTTATCGGGTTTCGCCCGATGGGCGCCTTACTTTCTTTTACTTGCATAAAAGAAAGTAAGCAAAGAAAAGTGCACCCCAGCTTCACCGCTTATCCTCGCTc                                                                                                                                                         <  1:797246/251‑1 (MQ=255)                                                                                       cACTTCACTCGCACGAACTGGGAAAAAATGCCGCAGTCATCGGCGAAGTGACGACGGATAAAAAAGTGAGATTGTTGGGCAGCTTCGGGCAGTCACGGCTGTTGGATTTGCCGGTGAATGAGCCGTTACCAAGGATTTGTTGATTTTTTATCGGGTTTCGCCCGATGGGCGCCTTACTTTCTTTTACTTGCATAAAAGAAAGTAAGCAAAGAAAAGTGCACCCCAGCTTCACCGCTTATCCTCGCTCACtt                                                                                                                                                     >  1:336880/1‑251 (MQ=255)                                                                                       cACTTCACTCGCACGAACTGGGAAAAAATGCCGCAGTCATCGGCGAAGTGACGACGGATAAAAAAGCGAGATTGTTGGGCAGCTTCGGGCAGTCACGGCTGTTGGATTTGCCGGTGAATGAGCCGTTACCAAGGATTTGTTGATTTTTTATCGGGTTTCGCCCGATGGGCGCCTTACTTTCTTTTACTTGCATAAAAGAAAGTAAGCAAAGAAAAGTGCACCCCAGCTTCACCGCTTATCCTCGCTCACtt                                                                                                                                                     >  1:659976/1‑251 (MQ=255)                                                                                           tCACTCGCACGAACTGGGAAAAAATGCCGCAGTCATCGGCGAAGTGACGACGGATAAAAAAGTGAGATTGTTGGGCAGCTTCGGGCAGTCACGGCTGTTGGATTTGCCGGTGAATGAGCCGTTACCAAGGATTTGTTGATTTTTTATCGGGTTTCGCCCGATGGGCGCCTTACTTTCTTTTACTTGCATAAAAGAAAGTAAGCAAAGAAAAGTGCACCCCAGCTTCACCGCTTATCCTCGCTCACTTGCaa                                                                                                                                                 >  1:874176/1‑251 (MQ=255)                                                                                                                     ccGCAGTCATCGGCGAAGTGACGACGGATAAAAAAGTGAGATTGTTGGGCAGCTTCGGGCAGTCACGGCTGTTGGATTTGCCGGTGAATGAGCCGTTACCAAGGATTTGTTGATTTTTTATCGGGTTTCGCCCGATGGGCGCCTTACTTTCTTTTACTTGCATAAAAGAAAGTAAGCAAAGAAAAGTGCACCCCAGCTTCACCGCTTATCCTCGCTCACTTGCAATTTTCTTAACGAAAATTTTCGAACt                                                                                                                        >  1:393669/1‑250 (MQ=255)                                                                                                                          gTCATCGGCGAAGTGACGACGGATAAAAAAGTGAGATTGTTGGGCAGCTTCGGGCAGTCACGGCTGTTGGATTTGCCGGTGAATGAGCCGTTACCAAGGATTTGTTGATTTTTTATCGGGTTTCGCCCGATGGGCGCCTTACTTTCTTTTACTTGCATAAAAGAAAGTAAGCAAAGAAAAGTGCACCCCAGCTTCACCGCtt                                                                                                                                                                   >  1:532728/1‑202 (MQ=255)                                                                                                                               cGGCGAAGTGACGACGGATAAAAAAGTGAGATTGTTGGGCAGCTTCGGGCAGTCACGGCTGTTGGATTTGCCGGTGAATGAGCCGTTACCAAGGATTTGTTGATTTTTTATCGGGTTTCGCCCGATGGGCGCCTTACTTTCTTTTACTTGCATAAAAGAAAGTAAGCAAAGAAAAGTGCACCCCAGCTTCACCGCTTATCCTCGCTCACTTGCAATTTTCTTAACGAAAATTTTCGAACTCGCCATTTCAt                                                                                                             <  1:118208/251‑1 (MQ=255)                                                                                                                               cGGCGAAGTGACGACGGATAAAAAAGTGAGATTGTTGGGCAGCTTCGGGCAGTCACGGCTGTTGGATTTGCCGGTGAATGAGCCGTTACCAAGGATTTGTTGATTTTTTATCGGGTTTCGCCCGATGGGCGCCTTACTTTCTTTTACTTGCATAAAAGAAAGTAAGCAAAGAAAAGTGCACCCCAGCTTCACCGCTTATCCTCGCTCACTTGCAATTTTCTTAACGAAAATTTTCGAACTCGCCATTTCAt                                                                                                             <  1:884414/251‑1 (MQ=255)                                                                                                                                ggCGAAGTGACGACGGATAAAAAAGTGAGATTGTTGGGCAGCTTCGGGCAGTCACGGCTGTTGGATTTGCCGGTGAATGAGCCGTTACCAAGGATTTGTTGATTTTTTATCGGGTTTCGCCCGATGGGCGCCTTACTTTCTTTTACTTGCATAAAAGAAAGTAAGCAAAGAAAAGTGCACCCCAGCTTCACCGCTTATCCTCGCTCACTTGCAATTTTCTTAACGAAAATTTTCGAACTCGCCATTTCATg                                                                                                            >  1:470600/1‑251 (MQ=255)                                                                                                                                  cGAAGTGACGACGGATAAAAAAGTGAGATTGTTGGGCAGCTTCGGGCAGTCACGGCTGTTGGATTTGCCGGTGAATGAGCCGTTACCAAGGATTTGTTGATTTTTTATCGGGTTTCGCCCGATGGGCGCCTTACTTTCTTTTACTTGCATAAAAGAAAGTAAGCAAAGAAAAGTGCACCCCAGCTTCACCGCTTATCCTCGCTCACTTGCAATTTTCTTAACGAAAATTTTCGAACTCGCCATTTCATGGc                                                                                                          <  1:206305/251‑1 (MQ=255)                                                                                                                                     aGTGACGACGGATAAAAAAGTGAGATTGTTGGGCAGCTTCGGGCAGTCACGGCTGTTGGATTTGCCGGTGAATGAGCCGTTACCAAGGATTTGTTGATTTTTTATCGGGTTTCGCCCGATGGGCGCCTTACTTTCTTTTACTTGCATAAAAGAAAGTAAGCAAAGAAAAGTGCACCCCAGCTTCACCGCTTATCCt                                                                                                                                                              >  1:140900/1‑196 (MQ=255)                                                                                                                                      gTGACGACGGATAAAAAAGTGAGATTGTTGGGCAGCTTCGGGCAGTCACGGCTGTTGGATTTGCCGGTGAATGAGCCGTTACCAAGGATTTGTTGATTTTTTATCGGGTTTCGCCCGATGGGCGCCTTACTTTCTTTTACTTGCATAAAAGAAAGTAAGCAAAGAAAAGTGCACCCCAGCTTCACCGCTTATCCTCGCTCACTTGCAATTTTCTTAACGAAAATTTTCGAACTCGCCATTTCATGGCTCaa                                                                                                      >  1:308490/1‑251 (MQ=255)                                                                                                                                              ggATAAAAAAGTGAGATTGTTGGGCAGCTTCGGGCAGTCACGGCTGTTGGATTTGCCGGTGAATGAGCCGTTACCAAGGATTTGTTGATTTTTTATCGGGTTTCGCCCGATGGGCGCCTTActttcttt                                                                                                                                                                                                                        >  1:424748/1‑129 (MQ=255)                                                                                                                                              ggATAAAAAAGTGAGATTGTTGGGCAGCTTCGGGCAGTCACGGCTGTTGGATTTGCCGGTGAATGAGCCGTTACCAAGGATTTGTTGATTTTTTATCGGGTTTCGCCCGATGGGCGCCTTACTTTCTTTTACTTGCATAAAAGAAAGTAAGCAAAGAAAAGTGCACCCCAGCTTCACCGCTTATCCTCGCTCACTTGCAATTTTCTTAACGAAAATTTTCGAACTCGCCATTTCATGGCTCAAACAGGCGa                                                                                              >  1:482553/1‑251 (MQ=255)                                                                                                                                               gATAAAAAAGTGAGATTGTTGGGCAGCTTCGGGCAGTCACGGCTGTTGGATTTGCCGGTGAATGAGCCGTTACCAAGGATTTGTTGATTTTTTATCGGGTTTCGCCCGATGGGCGCCTTAc                                                                                                                                                                                                                               <  1:536416/121‑1 (MQ=255)                                                                                                                                               gATAAAAAAGTGAGATTGTTGGGCAGCTTCGGGCAGTCACGGCTGTTGGATTTGCCGGTGAATGAGCCGTTACCAAGGATTTGTTGATTTTTTATCGGGTTTCGCCCGATGGGCGCCTTACTTTCTTTTACTTGCATAAAAGAAAGTAAGCAAAGAAAAGTGCACCCCAGCTTCACCGCTTATCCt                                                                                                                                                              <  1:483466/186‑1 (MQ=255)                                                                                                                                               gATAAAAAAGTGAGATTGTTGGGCAGCTTCGGGCAGTCACGGCTGTTGGATTTGCCGGTGAATGAGCCGTTACCAAGGATTTGTTGATTTTTTATCGGGTTTCGCCCGATGGGCGCCTTACTTTCTTTTACTTGCATAAAAGAAAGTAAGCAAAGAAAAGTGCACCCCAGCTTCACAGCTTATCCTCGCTCACTTGCAATTTTCTTAACGAAAATTTTCGAACTCGCCATTTCATGGCTCAAACAGGCGaa                                                                                             >  1:890290/1‑251 (MQ=255)                                                                                                                                               gATAAAAAAGTGAGATTGTTGGGCAGCTTCGGGCAGTCACGGCTGTTGGATTTGCCGGTGAATGAGCCGTTACCAAGGATTTGGTGATTTTTTATCGGGTTTCGCCCGATGGGCGCCTTACTTTCTTTTACTTGCATAAAAGAAAGTAAGCAAAGAAAAGTGCACCCCAGCTTCACCGCTTATCCTCGCTCACTTGCAATTTTCTTAACGAAAATTTTCGAACTCGCCATTTCATGGCTCAAACAGGCGaa                                                                                             >  1:373836/1‑251 (MQ=255)                                                                                                                                                      aaGTGAGATTGTTGGGCAGCTTCGGGCAGTCACGGCTGTTGGATTTGCCGGTGAATGAGCCGTTACCAAGGATTTGTTGATTTTTTATCGGGTTTCGCCCGATGGGCGCCTTACTTTCTTTTACTTGCATAAAAGAAAGTAAGCAAAGAAAAGTGCACCCCAGCTTCACCGCTTATCCTCGCTCACTTGCAATTTTCTTAACGAAAATTTTCGAACTCGCCATTTCATGGCTCAAACAGGCGAAAATTTc                                                                                       >  1:623634/1‑250 (MQ=255)                                                                                                                                                                        gCTTCGGGCAGTCACGGCTGTTGGATTTGCCGGTGAATGAGCCGTTACCAAGGATTTGTTGATTTTTTATCGGGTTTCGCCCGATGGGCGCCTTACTTTCTTTTACTTGCATAAAAGAAAGTAAGCAAAGAAAAGTGCACCCCAGCTTCACCGCTTATCCTCGCTCACTTGCaa                                                                                                                                                 >  1:892546/1‑174 (MQ=255)                                                                                                                                                                        gCTTCGGGCAGTCACGGCTGTTGGATTTGCCGGTGAATGAGCCGTTACCAAGGATTTGTTGATTTTTTATCGGGTTTCGCCCGATGGGCGCCTTACTTTCTTTTACTTGCATAAAAGAAAGTAAGCAAAGAAAAGTGCACCCCAGCTTCACCGCTTATCCTCGCTCACTTGCAATTTTCTTAACGAAAATTTTCGAACTCGCCATTTCATGGCTCAAACAGGCGAAAATTTCCTAAAAATTGCAAATCg                                                                      >  1:803936/1‑249 (MQ=255)                                                                                                                                                                             gggCAGTCACGGCTGTTGGATTTGCCGGTGAATGAGCCGTTACCAAGGATTTGTTGATTTTTTATCGGGTTTCGCCCGATGGGCGCCTTACTTTCTTTTACTTGCATAAAAGAAAGTAAGCAAAGAAAAGTGCACCCCAGCTTCACCGCTTATCCTCGCTCACTTGCAATTTTCTTAACGAAAATTTTCGAACTCGCCATTTCATGGCTc                                                                                                        >  1:272238/1‑210 (MQ=255)                                                                                                                                                                              ggCAGTCACGGCTGTTGGATTTGCCGGTGAATGAGCCGTTACCAAGGATTTGTTGATTTTTTATCGGGTTTCGCCCGATGGGCGCCTTACTTTCTTTTACTTGCATAAAAGAAAGTAAGCAAAGAAAAGTGCACCCCAGCTTCACCGCTTATCCTCGCTCACTTGCaa                                                                                                                                                 <  1:499393/168‑1 (MQ=255)                                                                                                                                                                              ggCAGTCACGGCTGTTGGATTTGCCGGTGAATGAGCCGTTACCAAGGATTTGTTGATTTTTTATCGGGTTTCGCCCGATGGGCGCCTTACTTTCTTTTACTTGCATAAAAGAAAGTAAGCAAAGAAAAGTGCACCCCAGCTTCACCGCTTATCCTCGCTCACTTGCAATTTTCTTAACGAAAATTTTCGAACTCGCCATTTCATGGCTCAAACAGGCGAAAATTTCCTAAAAATTGCAAATCGCTCAggc                                                               <  1:198116/250‑1 (MQ=255)                                                                                                                                                                                  gTCACGGCTGTTGGATTTGCCGGTGAATGAGCCGTTACCAAGGATTTGTTGATTTTTTATCGGGTTTCGCCCGATGGGCGCCTTACTTTCTTTTACTTGCATAAAAGAAAGTAAGCAAAGAAAAGTGCACCCCAGCTTCACCGCTTATCCTCGCTCACTTGCAATTTTCTTAACGAAAATTTTCGAACTCGCCATTTCATGGCTCAAACAGGCGAAAATTTCCTAAAAATTGCAAATCGCTCAGGCGGCt                                                           >  1:595119/1‑250 (MQ=255)                                                                                                                                                                                   tCACGGCTGTTGGATTTGCCGGTGAATGAGCCGTTACCAAGGATTTGTTGATTTTTTATCGGGTTTCGCCCGATGGGCGCCTTACTTTCTTTTACTTGCATAAAAGAAAGTAAGCAAAGAAAAGTGCACCCCAGCTTCACCGCTTATCCTCGCTCACTTGCAATTTTCTTAACGAAAATTTTCGAACTCGCCATTTCATGGCTCAAACAGGCGAAAATTTCCTAAAAATTGCAAATCGCTCAGGCGGCTCa                                                         <  1:85191/251‑1 (MQ=255)                                                                                                                                                                                    cACGGCTGTTGGATTTGCCGGTGAATGAGCCGTTACCAAGGATTTGTTGATTTTTTATCGGGTTTCGCCCGATGGGCGCCTTACTTTCTTTTACTTGCATAAAAGAAAGTAAGCAAAGAAAAGTGCACCCCAGCTTCACCGCTTATCCTCGCTCACTTGCAATTTTCTTAACGAAAATTTTCGAACTCGCCATTTCATGGCTCAAACAGGCGAAAATTTCCTAAAAATTGCAAATCGCTCAGGCGGCTCAg                                                        <  1:81707/251‑1 (MQ=255)                                                                                                                                                                                        gCTGTTGGATTTGCCGGTGAATGAGCCGTTACCAAGGATTTGTTGATTTTTTATCGGGTTTCGCCCGATGGGCGCCTTACTTTCTTTTACTTGCATAAAAGAAAGTAAGCAAAGAAAAGTGCACCCCAGCTTCACCGCTTATCCTCGCTCACTTGCAATTTTCTTAACGAAAATTTTCGAACTCGCCATTTCATGGCTCAAACAGGCGAAAATTTCCTAAAAATTGCAAATCGCTCAGGCGGCTCAGATg                                                     <  1:616982/250‑1 (MQ=255)                                                                                                                                                                                                aTTTGCCGGTGAATGAGCCGTTACCAAGGATTTGTTGATTTTTTATCGGGTTTCGCCCGATGGGCGCCTTACTTTCTTTTACTTGCATAAAAGAAAGTAAGCAAAGAAAAGTGCACCCCAGCTTCACCGCTTATCCTCGCTCACTTGCAATTTTCTTAACGAAAATTTTCGAACTCGCCATTTCATGGCTCAAACAGGCGAAAATTTCCTAAAAATTGCAAATCGCTCAGGCGGCTCAGATGGGGGccccc                                            <  1:901151/251‑1 (MQ=255)                                                                                                                                                                                                    gCCGGTGAATGAGCCGTTACCAAGGATTTGTTGATTTTTTATCGGGTTTCGCCCGATGGGCGCCTTACTTTCTTTTACTTGCATAAAAGAAAGTAAGCAAAGAAAAGTGCACCCCAGCTTCACCGCTTATCCTCGCTCACTTGCAATTTTCTTAACGAAAATTTTCGAACTCGCCATTTCATGGCTCAAACAGGCGAAAATTTCCTAAAAATTGCAAATCGCTCAGGCGGCTCAGATGGGGGCCCCCTGtt                                        >  1:585628/1‑251 (MQ=255)                                                                                                                                                                                                          gAATGAGCCGTTACCAAGGATTTGTTGATTTTTTATCGGGTTTCGCCCGATGGGCGCCTTACTTTCTTTTACTTGCATAAAAGAAAGTAAGCAAAGAAAAGTGCACCCCAGCTTCACCGCTTATCCTCGCTCACTTGCAATTTTCTTAACGAAAATTTTCGAACTCGCCATTTCATGGCTCAAACAGGCGAAAATTTCCTAAAAATTGCAAATCGCTCAGGCGGCTCAGATGGGGGCCCCCTGTTTTGTCg                                  >  1:855719/1‑251 (MQ=255)                                                                                                                                                                                                          gAATGAGCCGTTACCAAGGATTTGTTGATTTTTTATCGGGTTTCGCCCGATGGGCGCCTTACTTTCTTTTACTTGCATAAAAGAAAGTAAGCAAAGAAAAGTGCACCCCAGCTTCACCGCTTATCCTCGCTCACTTGCAATTTTCTTAACGAAAATTTTCGAACTCGCCATTTCATGGCTCAAACAGGCGAAAATTTCCTAAAAATTGCAAATCGCTCAGGCGGCTCAGATGGGGGCCCCCTGTTTTGTCg                                  >  1:124411/1‑251 (MQ=255)                                                                                                                                                                                                              gAGCCGTTACCAAGGATTTGTTGATTTTTTATCGGGTTTCGCCCGATGGGCGCCTTACTTTCTTTTACTTGCATAAAAGAAAGTAAGCAAAGAAAAGTGCACCCCAGCTTCACCGCTTATCCTCGCTCACTTGCAATTTTCTTAACGAAAATTTTCGAACTCGCCATTTCATGGCTCAAACAGGCGAAAATTTCCTAAAAATTGCAAATCGCTCAGGCGGCTCAGATGGGGGCCCCCTGTTTTGTCGGAg                               >  1:673689/1‑250 (MQ=255)                                                                                                                                                                                                                   gTTACCAAGGATTTGTTGATTTTTTATCGGGTTTCGCCCGATGGGCGCCTTACTTTCTTTTACTTGCATAAAAGAAAGTAAGCAAAGAAAAGTGCACCCCAGCTTCACCGCTTATCCTCGCTCACTTGCAATTTTCTTAACGAAAATTTTCGAACTCGCCATTTCATGGCTCAAACAGGCGAAAATTTCCTAAAAATTGCAAATCGCTCAGGCGGCTCAGATGGGGGCCCCCTGTTTTGTCGGAGCATCaa                         >  1:404058/1‑251 (MQ=255)                                                                                                                                                                                                                      aCCAAGGATTTGTTGATTTTTTATCGGGTTTCGCCCGATGGGCGCCTTACTTTCTTTTACTTGCATAAAAGAAAGTAAGCAAAGAAAAGTGCACCCCAGCTTCACCGCTTATCCTCGCTCACTTGCAATTTTCTTAACGAAAATTTTCGAACTCGCCATTTCATGGCTCAAACAGGCGAAAATTTCCTAAAAATTGCAAATCGCTCAGGCGGCTCAGATGGGGGCCCCCTGTTTTGTCGGAGCATCaaaaa                      >  1:70814/1‑251 (MQ=255)                                                                                                                                                                                                                      aCCAAGGATTTGTTGATTTTTTATCGGGTTTCGCCCGATGGGCGCCTTACTTTCTTTTACTTGCATAAAAGAAAGTAAGCAAAGAAAAGTGCACCCCAGCTTCACCGCTTATCCTCGCTCACTTGCAATTTTCTTAACGAAAATTTTCGAACTCGCCATTTCATGGCTCAAACAGGCGAAAATTTCCTAAAAATTGCAAATCGCTCAGGCGGCTCAGATGGGGGCCCCCTGTTTTGTCGGAGCATCaaaaa                      <  1:240921/251‑1 (MQ=255)                                                                                                                                                                                                                        cAAGGATTTGTTGATTTTTTATCGGGTTTCGCCCGATGGGCGCCTTACTTTCTTTTACTTGCATAAAAGAAAGTAAGCAAAGAAAAGTGCACCCCAGCTTCACCGCTTATCCTCGCTCACTTGCAATTTTCTTAACGAAAATTTTCGAACTCGCCATTTCGTGGCTCAAACAGGCGAAAATTACCTAAAAATTGCAAATCGCTCAGGCGGCTCAGATGGGGGCCCCCTGTTTTGTCGGAGCATCAAAAAAg                    <  1:364378/251‑1 (MQ=255)                                                                                                                                                                                                                            gATTTGTTGATTTTTTATCGGGTTTCGCCCGATGGGCGCCTTACTTTCTTTTACTTGCATAAAAGAAAGTAAGCAAAGAAAAGTGCACCCCAGCTTCACCGCTTATCCTCGCTCACTTGCAATTTTCTTAACGAAAATTTTCGAACTCGCCATTTCATGGCTCAAACAGGCGAAAATTTCCTAAAAATTGCAAATCGCTCAGGCGGCTCAGATGGGGGCCCCCTGTTTTGt                                    <  1:820747/231‑1 (MQ=255)                                                                                                                                                                                                                                          ttATCGGGTTTCGCCCGATGGGCGCCTTACTTTCTTTTACTTGCATAAAAGAAAGTAAGCAAAGAAAAGTGCACCCCAGCTTCACCGCTTATCCTCGCTCACTTGCAATTTTCTTAACGAAAATTTTCGAACTCGCCATTTCATGGCTCAAACAGGCGAAAATTTCCTAAAAATTGCAAATCGCTCAGGCGGCTCAGATGGGGGCCCCCTGTTTTGTCGGAGCATCAAAAAAGTGCGGTACATTTTGAAAt  <  1:885116/251‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                GCTCGGCTTAGAAGCGCTGAATTTTGCTAACGAAGGCAAACTGGTCATCATCGCCGCCCCTGAAAAAACACCTGAAATTCTGACCGCACTTCACTCGCACGAACTGGGAAAAAATGCCGCAGTCATCGGCGAAGTGACGACGGATAAAAAAGTGAGATTGTTGGGCAGCTTCGGGCAGTCACGGCTGTTGGATTTGCCGGTGAATGAGCCGTTACCAAGGATTTGTTGATTTTTTATCGGGTTTTGCCCGATGGGCGCCTTACTTTCTTTTACTTGCATAAAAGAAAGTAAGCAAAGAAAAGTGCACCCCAGCTTCACCGCTTATCCTCGCTCACTTGCAATTTTCTTAACGAAAATTTTCGAACTCGCCATTTCATGGCTCAAACAGGCGAAAATTTCCTAAAAATTGCAAATCGCTCAGGCGGCTCAGATGGGGGCCCCCTGTTTTGTCGGAGCATCAAAAAAGTGCGGTACATTTTGAAAT  >  1/1222822‑1223305

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑