Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	778,162	G→C	intergenic (+43230/+43715)	unknown → / ← unknown	tRNA‑Asn‑GTT/tRNA‑Thr‑GGT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	778,162	0	G	C	100.0%	117.2 / NA	36	intergenic (+43230/+43715)	unknown/unknown	tRNA‑Asn‑GTT/tRNA‑Thr‑GGT
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (19/17);  total (19/17)

TGATGTTAAATAACAGCAAACCGATTATGTGTAATAAGGCGGTGTATTACAACGACCCTTATTTCATCGATAAATTGAAAGCGGTTTCCCCACAACTCCGCGCATTAGGCAAGAAATTACCGACTAAAGCGCAACTGGAAAAAGCCATGTTCAGTGGTGAGCTTTCCGCCCAATCTATTTAATCCACAAAAACTGTGAATAACCAGGAGGATAATATGAAAAAATTTTTAGTTATGAATACGTTAATCCCTGTTTGCTTTTTAAGCGCGT‑GCAGCTCGTCAACGCCGGAACCGCCACAAATCGATGAAGACGCCCCGGCAATTTATTTAAATAATCAGGTGTACCGACAAACACCAACTGCAACAGTGCCAAAAAACGCCGGCGATCATAACGGGCAACCTTGGGTATATCAATATGTGAACTTAGATAGAAACGATTATGTGAATGAAACAGA  >  1/777932‑778385                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                 tgATGTTAAATAACAGCAAACCGATTATATGTAATAAGGCAGTGTATTACAACGATCCTTATTTTATAGACAAACTCAAAGCGGTATCTCCGCAATTGCGAGCATTAGGTAAAAAATTACCGACAAAAGAACAATTGGAAAAAGCTATGTTCAGTGGCGAGCTGTCAGTCCAATCAATTTAATCCACAAAAACTGTGAATAACCAGGAGGATAATATGAAAAAAATTTTACTTATGAATATGTTAAtacc                                                                                                                                                                                                               <  1:434082/250‑4 (MQ=255) tgATGTTAAATAACAGCAAACCGATTATATGTAATAAGGCAGTGTATTACAACGATCCTTATTTTATAGACAAACTCAAAGCGGTATCTCCGCAATTGCGAGCATTAGGTAAAAAATTACCGACAAAAGAACAATTGGAAAAAGCTATGTTCAGTGGCGAGCTGTCAGTCCAATCAATTTAATCCACAAAAACTGTGAATAACCAGGAGGATAATATGAAAAAAATTTTACTTATGAATATGTTAAtacc                                                                                                                                                                                                               <  1:403990/250‑4 (MQ=255)        aaaTAACAGCAAACCGATTATATGTAATAAGGCAGTGTATTACAACGATCCTTATTTTATAGACAAACTCAAAGCGGTATCTCCGCAATTGCGAGCATTAGGTAAAAAATTACCGACAAAAGAACAATTGGAAAAAGCTATGTTCAGTGGCGAGCTGTCAGTCCAATCAATTTAATCCACAAAAACTGTGAATAACCAGGAGGATAATATGAAAAAAATTTTACTTATg                                                                                                                                                                                                                             <  1:136376/229‑1 (MQ=255)                 cAAACCGATTATATGTAATAAGGCAGTGTATTACAACGATCCTTATTTTATAGACAAACTCAAAGCGGTATCTCCGCAATTGCGAGCATTAGGTAAAAAATTACCGACAAAAGAACAATTGGAAAAAGCTATGTTCAGTGGCGAGCTGTCAGTCCAATCAATTTAATCCACAAAAACTGTGAATAACCAGGAGGATAATATGAAAAAAATTTTACTTATGAATATGTTAATACCTGTTTGCTTTTTAAg                                                                                                                                                                                                <  1:41541/249‑1 (MQ=255)                     ccGATTATATGTAATAAGGCAGTGTATTACAACGATCCTTATTTTATAGACAAACTCAAAGCGGTATCTCCGCAATTGCGAGCATTAGGTAAAAAATTACCGACAAAAGAACAATTGGAAAAAGCTATGTTCAGTGGCGAGCTGTCAGTCCAATCAATTTAATCCACAAAAACTGTGAATAACCAGGAGGATAATATGAAAAAAATTTTACTTATGAATATGTTAAtac                                                                                                                                                                                                                >  1:636159/1‑227 (MQ=255)                             atgtAATAAGGCAGTGTATTACAACGATCCTTATTTTATAGACAAACTCAAAGCGGTATCTCCGCAATTGCGAGCATTAGGTAAAAAATTACCGACAAAAGAACAATTGGAAAAAGCTATGTTCAGTGGCGAGCTGTCAGTCCAATCAATTTAATCCACAAAAACTGTGAATAACCAGGAGGATAATATGAAAAAAATTTTACTTATGAATATGTTAATACCTGTTTGCTTTTTAAGCGCGT‑GCAGCTCGt                                                                                                                                                                                 >  1:412919/2‑251 (MQ=255)                                taataaGGCAGTGTATTACAACGATCCTTATTTTATAGACAAACTCAAAGCGGTATCTCCGCAATTGCGAGCATTAGGTAAAAAATTACCGACAAAAGAACAATTGGAAAAAGCTATGTTCAGTGGCGAGCTGTCAGTCCAATCAATTTAATCCACAAAAACTGTGAATAACCAGGAGGATAATATGAAAAAAATTTTACTTATGAATATGTTAAtacc                                                                                                                                                                                                               <  1:757953/219‑4 (MQ=255)                                taataaGGCAGTGTATTACAACGATCCTTATTTTATAGACAAACTCAAAGCGGTATCTCCGCAATTGCGAGCATTAGGTAAAAAATTACCGACAAAAGAACAATTGGAAAAAGCTATGTTCAGTGGCGAGCTGTCAGTCCAATCAATTTAATCCACAAAAACTGTGAATAACCAGGAGGATAATATGAAAAAAATTTTACTTATGAATATGTTAATACCTGTTTGCTTTTTAAGCGCGT‑GCAGCTCGTCa                                                                                                                                                                               >  1:165664/1‑250 (MQ=255)                                taataaGGCAGTGTATTACAACGATCCTTATTTTATAGACAAACTCAAAGCGGTATCTCCGCAATTGCGAGCATTAGGTAAAAAATTACCGACAAAAGAACAATTGGAAAAAGCTATGTTCAGTGGCGAGCTGTCAGTCCAATCAATTTAATCCACAAAAACTGTGAATAACCAGGAGGATAATATGAAAAAAATTTTACTTATGAATATGTTAATACCTGTTTGCTTTTTAAGCGCGT‑GCAGCTCGTCa                                                                                                                                                                               >  1:565257/1‑250 (MQ=255)                                 aataaGGCAGTGTATTACAACGATCCTTATTTTATAGACAAACTCAAAGCGGTATCTCCGCAATTGCGAGCATTAGGTAAAAAATTACCGACAAAAGAACAATTGGAAAAAGCTATGTTCAGTGGCGAGCTGTCAGTCCAATCAATTTAATCCACAAAAACTGTGAATAACCAGGAGGATAATATGAAAAAAATTTTACTTATGAATATGTTAATACCTGTTTGCTTTTTAAGCGCGT‑GCAGCTCGTCacc                                                                                                                                                                             <  1:646724/251‑3 (MQ=255)                                            gtATTACAACGATCCTTATTTTATAGACAAACTCAAAGCGGTATCTCCGCAATTGCGAGCATTAGGTAAAAAATTACCGACAAAAGAACAATTGGAAAAAGCTATGTTCAGTGGCGAGCTGTCAGTCCAATCAATTTAATCCACAAAAACTGTGAATAACCAGGAGGATAATATGAAAAAAATTTTACTTATGAATATGTTAAtacc                                                                                                                                                                                                               <  1:614625/207‑4 (MQ=255)                                            gtATTACAACGATCCTTATTTTATAGACAAACTCAAAGCGGTATCTCCGCAATTGCGAGCATTAGGTAAAAAATTACCGACAAAAGAACAATTGGAAAAAGCTATGTTCAGTGGCGAGCTGTCAGTCCAATCAATTTAATCCACAAAAACTGTGAATAACCAGGAGGATAATATGAAAAAAATTTTACTTATGAATATGTTAAtac                                                                                                                                                                                                                <  1:745828/206‑3 (MQ=255)                                            gtATTACAACGATCCTTATTTTATAGACAAACTCAAAGCGGTATCTCCGCAATTGCGAGCATTAGGTAAAAAATTACCGACAAAAGAACAATTGGAAAAAGCTATGTTCAGTGGCGAGCTGTCAGTCCAATCAATTTAATCCACAAAAACTGTGAATAACCAGGAGGATAATATGAAAAAAATTTTACTTATGAATATGTTAAtac                                                                                                                                                                                                                >  1:669767/1‑204 (MQ=255)                                            gtATTACAACGATCCTTATTTTATAGACAAACTCAAAGCGGTATCTCCGCAATTGCGAGCATTAGGTAAAAAATTACCGACAAAAGAACAATTGGAAAAAGCTATGTTCAGTGGCGAGCTGTCAGTCCAATCAATTTAATCCACAAAAACTGTGAATAACCAGGAGGATAATATGAAAAAAATTTTACTTATGAATATGTTAATACCTGTTTGCTTTTTAAg                                                                                                                                                                                                >  1:466134/1‑222 (MQ=255)                                            gtATTACAACGATCCTTATTTTATAGACAAACTCAAAGCGGTATCTCCGCAATTGCGAGCATTAGGTAAAAAATTACCGACAAAAGAACAATTGGAAAAAGCTATGTTCAGTGGCGAGCTGTCAGTCCAATCAATTTAATCCACAAAAACTGTGAATAACCAGGAGGATAATATGAAAAAAATTTTACTTATGAATATGTTAATACCTGTTTGCTTTTTAAGCGCGT‑GCAGCTCGTCaccaccagaac                                                                                                                                                                     >  1:643527/1‑238 (MQ=255)                                            gtATTACAACGATCCTTATTTTATAGACAAACTCAAAGCGGTATCTCCGCAATTGCGAGCATTAGGTAAAAAATTACCGACAAAAGAACAATTGGAAAAAGCTATGTTCAGTGGCGAGCTGTCAGTCCAATCAATTTAATCCACAAAAACTGTGAATAACCAGGAGGATAATATGAAAAAAATTTTACTTATGAATATGTTAATACCTGTTTGCTTTTTAAGCGCGT‑GCAGCTCGTCac                                                                                                                                                                              <  1:504059/239‑2 (MQ=255)                                            gtATTACAACGATCCTTATTTTATAGACAAACTCAAAGCGGTATCTCCGCAATTGCGAGCATTAGGTAAAAAATTACCGACAAAAGAACAATTGGAAAAAGCTATGTTCAGTGGCGAGCTGTCAGTCCAATCAATTTAATCCACAAAAACTGTGAATAACCAGGAGGATAATATGAAAAAAATTTTACTTATGAATATGTTAATACCTGTTTGCTTTTTAAGCGCGT‑GCAGCTCGTCACCACCAGAACCg                                                                                                                                                                   >  1:659897/1‑250 (MQ=255)                                             tATTACAACGATCCTTATTTTATAGACAAACTCAAAGCGGTATCTCCGCAATTGCGAGCATTAGGTAAAAAATTACCGACAAAAGAACAATTGGAAAAAGCTATGTTCAGTGGCGAGCTGTCAGTCCAATCAATTTAATCCACAAAAACTGTGAATAACCAGGAGGATAATATGAAAAAAATTTTACTTATGAATATGTTAATACCTGTTTGCTTTTTAAGCGCGT‑GCAGCTCGTCACCACCAGAACCGc                                                                                                                                                                  <  1:817973/250‑1 (MQ=255)                                              aTTACAACGATCCTTATTTTATAGACAAACTCAAAGCGGTATCTCCGCAATTGCGAGCATTAGGTAAAAAATTACCGACAAAAGAACAATTGGAAAAAGCTATGTTCAGTGGCGAGCTGTCAGTCCAATCAATTTAATCCACAAAAACTGTGAATAACCAGGAGGATAATATGAAAAAAATTTTACTTATGAATATGtt                                                                                                                                                                                                                     <  1:467659/199‑1 (MQ=255)                                                       atccTTATTTTATAGACAAACTCAAAGCGGTATCTCCGCAATTGCGAGCATTAGGTAAAAAATTACCGACAAAAGAACAATTGGAAAAAGCTATGTTCAGTGGCGAGCTGTCAGTCCAATCAATTTAATCCACAAAAACTGTGAATAACCAGGAGGATAATATGAAAAAAATTTTACTTATGAATATGTTAAtacc                                                                                                                                                                                                               <  1:777520/194‑4 (MQ=255)                                                        tccTTATTTTATAGACAAACTCAAAGCGGTATCTCCGCAATTGCGAGCATTAGGTAAAAAATTACCGACAAAAGAACAATTGGAAAAAGCTATGTTCAGTGGCGAGCTGTCAGTCCAATCAATTTAATCCACAAAAACTGTGAATAACCAGGAGGATAATATGAAAAAAATTTTACTTATGAATATGTTAATACCTGTTTGCTTTTTAAGCGCGT‑GCAGCTCGTCACCACCAGAACCGCCGCAAATTGAta                                                                                                                                                      >  1:274096/2‑250 (MQ=255)                                                                                        ctccgcaattgcgagcATTAGGTAAAAAATTACCGACAAAAGAACAATTGGAAAAAGCTATGTTCAGTGGCGAGCTGTCAGTCCAATCAATTTAATCCACAAAAACTGTGAATAACCAGGAGGATAATATGAAAAAAATTTTACTTATg                                                                                                                                                                                                                             >  1:326950/15‑149 (MQ=255)                                                                                                                    attaccgaaaaaagaacaatgggAAAAAGTTATGTTCAGTGGCGAGCTGTCAGTCCAATAAATTTAATCCAAAAAAACTGTGAAAAACCAGGAGGATAATATGAAAAAAATTTTACTTATGAATATGTTAATCCCTGTTTGCTTTTTAAGCGCGT‑GCAGCTCGTCACCACCAGAACCGCCGCAAATTGATAAAGACGCCCCAGTAATCCATTTAAATAATCAGGTGTACCGACAAACACCAACTGCAACAg                                                                                          <  1:896182/230‑1 (MQ=255)                                                                                                                                 gaacaattGGAAAAAGCTATGTTCAGTGGCGAGCTGTCAGTCCAATCAATTTAATCCACAAAAACTGTGAATAACCAGGAGGATAATATGAAAAAAATTTTACTTATGAATATGTTAATACCTGTTTGCTTTTTAAGCGCGT‑GCAGCTCGTCACCACCAGAACCGCCGCAAATTGATAAAGACGCCCCAGTAATCCATTTAAATAATCAGGTGTACCGACAAACACCAACTGCAACAGTGCCAAAAAACg                                                                              >  1:342336/8‑250 (MQ=255)                                                                                                                                      attGGAAAAAGCTATGTTCAGTGGCGAGCTGTCAGTCCAATCAATTTAATCCACAAAAACTGTGAATAACCAGGAGGATAATATGAAAAAAATTTTACTTATGAATATGTTAATACCTGTTTGCTTTTTAAGCGCGT‑GCAGCTCGTCACCACCAGAACCGCCGCAAATTGATAAAGACGccccagtaa                                                                                                                                       >  1:882833/3‑183 (MQ=255)                                                                                                                                              aaGCTATGTTCAGTGGCGAGCTGTCAGTCCAATCAATTTAATCCACAAAAACTGTGAATAACCAGGAGGATAATATGAAAAAAATTTTACTTATGAATATGTTAATACCTGTTTGCTTTTTAAGCGCGT‑GCAGCTCGTCACCACCAGAACCGCCGCAAATTGATAAAGACGccccagtaatcc                                                                                                                                    >  1:8297/1‑175 (MQ=255)                                                                                                                                                 ctaTGTCCAGTGGCGAGCTGTCAGTCCAAACAATTTAACCCAAAAAAACGGTGAATAACAAGGAGAAAAATAAAAAAAAAATTTTACTTATAAATATTTTAATCCCTGTTTGTTTTTAAAGCGCGT‑GCAGCTCGTCCCCACCAGAACCGCCGCAAATTGATAAAGACGCCCCAGTAATCCATTTAAATAATCAGGTGTACCGACAAACACCAACTGCAACAGTGCCAAAAAACGCCGGCGATCATAATggg                                                             <  1:880328/249‑1 (MQ=255)                                                                                                                                                     gTTCAGTGGCGAGCTGTCAGTCCAATCAATTTAATCCACAAAAACTGTGAATAACCAGGAGGATAATATGAAAAAAATTTTACTTATGAATATGTTAATACCTGTTTGCTTTTTAAGCGCGT‑GCAGCTCGTCACCACCAGAACCGCCGCAAATTGATAAAGACGCCCCAGTAATCCATTTAAATAATCAGGTGTACCGACAAACACCAACTGCAACAGTGCCAAAAAACGCCGGCGATCATAATGGGCaa                                                          >  1:145306/1‑250 (MQ=255)                                                                                                                                                     gTTCAGTGGCGAGCTGTCAGTCCAATCAATTTAATCCACAAAAACTGTGAATAACCAGGAGGATAATATGAAAAAAATTCTACTTATGAATATGTTAATACCTGTTTGCTTTTTAAGCGCGT‑GCAGCTCGTCACCACCAGAACCGCCGCAAATTGATAAAGACGCCCCAGTAATCCATTTAAATAATCAGGTGTACCGACAAACACCAACTGCAACAGTGCCAAAAAACGCCGGCGATCATAATGGGCaa                                                          >  1:638072/1‑250 (MQ=255)                                                                                                                                                                  ctgtcagtccaataaattaaaTCAACAAAAACTGTGAAAAACCAGGAGAAAAATAAAAAAAAAATTTTACTTATGAATATGTTAATCCCTGTTTCCTTTTAAGGCG‑GTGGCAGCTCGACACCACCAAAACCGCCGCAAATTGATAAAGACGCCCCAGTAATCCATTTAAATAATCAGGTGTACCGACAAACACCAACTGCAACAGTGCCAAAAAACGCCGGCGATCATAATGGGCAACCTTGGGTATATc                                             <  1:408089/231‑1 (MQ=255)                                                                                                                                                                           caatcaatTTAATCCACAAAAACTGTGAATAACCAGGAGGATAATATGAAAAAAATTTTACTTATGAATATGTTAATACCTGTTTGCTTTTTAAGCGCGT‑GCAGCTCGTCACCACCAGAACCGCCGCAAATTGATAAAGACGCCCCAGTAATCCATTTAAATAATCAGGTGTACCGACAAACACCAACTGCAACAGTGCCAAAAAACGCCGGCGATCATAATGGGCAACCTTGGGTATATCAATAtg                                       >  1:393289/1‑247 (MQ=255)                                                                                                                                                                                                aCTGTGAATAACCAGGAGGATAATATGAAAAAAATTTTACTTATGAATATGTTAATACCTGTTTGCTTTTTAAGCGCGT‑GCAGCTCGTCACCACCAGAACCGCCGCAAATTGATAAAGACGCCCCAGTAATCCATTTAAATAATCAGGTGTACCGACAAACACCAACTGCAACAGTGCCAAAAAACGCCGGCGATCATAATGGGCAACCTTGGGTATATCAATATGTGAACCTAGGTAGAAGCGATTAtgt              >  1:58895/1‑251 (MQ=255)                                                                                                                                                                                                 cTGTGAATAACCAGGAGGATAATATGAAAAAAATTTTACTTATGAATATGTTAATACCTGTTTGCTTTTTAAGCGCGT‑GCAGCTCGTCACCACCAGAACCGCCGCAAATTGATAAAGACGCCCCAGTAATCCATTTAAATAATCAGGTGTACCGACAAACACCAACTGCAACAGTGCCAAAAAACGCCGGCGATCATAATGGGCAACCTTGGGTATATCAATATGTGAACCTAGGTAGAAGCGATTAtgtg             >  1:398930/1‑251 (MQ=255)                                                                                                                                                                                                            cAGGAGGATAATATGAAAAAAATTTTACTTATGAATATGTTAATACCTGTTTGCTTTTTAAGCGCGT‑GCAGCTCGTCACCACCAGAACCGCCGCAAATTGATAAAGACGCCCCAGTAATCCATTTAAATAATCAGGTGTACCGACAAACACCAACTGCAACAGTGCCAAAAAACGCCGGCGATCATAATGGGCAACCTTGGGTATATCAATATGTGAACCTAGGTAGAAGCGATTATGTGAATGAAACAGa  >  1:22401/1‑251 (MQ=255)                                                                                                                                                                                                               gaggaTAATATAAAAAAAATTTTACTTATGAATATGTTAATCCCTGTTTGCTTTTTAAGCGCGT‑GCAGCTCGTCACCACCAGAACCGCCGCAAATTGATAAAGACGCCCCAGTAATCCATTTAAATAATCAGGTGTACCGACAAacac                                                                                                      <  1:431333/148‑1 (MQ=255)                                                                                                                                                                                                                 ggaTAATATAAAAAAAATTTTACTTATAAATATGTTAATCCCTGTTTGCTTTTTAAGCGCGT‑GCAGCTCGTCCCCACCAGAACCGCCGCAAATTGATAAAGACGCCCCAGTAATCCATTTAAATAATCAGGTGTACCGACAAACACCAACTGCAACAGTGCCAAAAAACGCCGGCGATCATaatg                                                               <  1:461020/185‑3 (MQ=255)                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                 TGATGTTAAATAACAGCAAACCGATTATGTGTAATAAGGCGGTGTATTACAACGACCCTTATTTCATCGATAAATTGAAAGCGGTTTCCCCACAACTCCGCGCATTAGGCAAGAAATTACCGACTAAAGCGCAACTGGAAAAAGCCATGTTCAGTGGTGAGCTTTCCGCCCAATCTATTTAATCCACAAAAACTGTGAATAACCAGGAGGATAATATGAAAAAATTTTTAGTTATGAATACGTTAATCCCTGTTTGCTTTTTAAGCGCGT‑GCAGCTCGTCAACGCCGGAACCGCCACAAATCGATGAAGACGCCCCGGCAATTTATTTAAATAATCAGGTGTACCGACAAACACCAACTGCAACAGTGCCAAAAAACGCCGGCGATCATAACGGGCAACCTTGGGTATATCAATATGTGAACTTAGATAGAAACGATTATGTGAATGAAACAGA  >  1/777932‑778385

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑