Predicted mutation
evidence seq id position mutation annotation gene description
RA 1 777,588 G→C intergenic (+42656/+44289) unknown → / ← unknown tRNA‑Asn‑GTT/tRNA‑Thr‑GGT

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*1777,5880GC98.6% 244.5 / NA 73intergenic (+42656/+44289)unknown/unknowntRNA‑Asn‑GTT/tRNA‑Thr‑GGT
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base C (30/42);  total (30/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AACCATTTTTCAAAATGAAATTGTTGAACATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGATGAACTACCGGAAACCAACCCGAAATTAAAATATCAGTTATTACTACTCATGGATGAGTTTACTGCGGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGCTATGGTTTACGCCTATTGATTATCTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGATAATATTGCCTGTAAAATTCTGTATGCTCCCGACAATCAGGAGGATGCAGAAGAATATTCTAAAGTGCTAGGTAATCGCACTGTAGATAAATTAA  >  1/777348‑777829
                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                 
aaCCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGt                                                                                                                                                                                                                                         <  1:570178/251‑1 (MQ=255)
  ccATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTg                                                                                                                                                                                                                                       <  1:518562/251‑1 (MQ=255)
  ccATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTg                                                                                                                                                                                                                                       >  1:285079/1‑251 (MQ=255)
     tttttCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGc                                                                                                                                                                                                                                    <  1:509584/251‑1 (MQ=255)
       tttCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCAt                                                                                                                                                                                                                                  <  1:632859/251‑1 (MQ=255)
          cAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTAt                                                                                                                                                                                                                               <  1:608420/251‑1 (MQ=255)
               tGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATa                                                                                                                                                                                                                          >  1:786649/1‑251 (MQ=255)
                      gttgAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCAt                                                                                                                                                                                                                                  <  1:598106/236‑1 (MQ=255)
                          aataTGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAgg                                                                                                                                                                                                                <  1:135605/247‑1 (MQ=255)
                                  aCTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGc                                                                                                                                                                                                       <  1:48175/251‑1 (MQ=255)
                                     tCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCgg                                                                                                                                                                                                    >  1:844166/1‑251 (MQ=255)
                                      cTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCggt                                                                                                                                                                                                   >  1:752925/1‑250 (MQ=255)
                                                        tACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTg                                                                                                                                                                                 <  1:112942/251‑1 (MQ=255)
                                                             gcTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATtat                                                                                                                                                                            >  1:534837/1‑251 (MQ=255)
                                                                         gAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAAt                                                                                                                                                                                                                           <  1:313429/192‑1 (MQ=255)
                                                                         gAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATcaca                                                                                                                                                                >  1:569374/1‑251 (MQ=255)
                                                                            aaaaTCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAGTATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAgg                                                                                                                                                             <  1:156221/251‑1 (MQ=255)
                                                                                 cACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAAc                                                                                                                                                        <  1:22072/251‑1 (MQ=255)
                                                                                   cGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACtt                                                                                                                                                      >  1:261/1‑251 (MQ=255)
                                                                                   cGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACtt                                                                                                                                                      <  1:833786/251‑1 (MQ=255)
                                                                                     atttatttCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCg                                                                                                                                                    >  1:399776/1‑251 (MQ=255)
                                                                                          tttCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTg                                                                                                                                                                                 >  1:653808/1‑217 (MQ=255)
                                                                                             ccACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGAt                                                                                                                                                                               >  1:51803/1‑216 (MQ=255)
                                                                                                        cAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATgg                                                                                                                                  <  1:840182/250‑1 (MQ=255)
                                                                                                             gATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGGGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGcc                                                                                                                                                                                      >  1:66129/1‑193 (MQ=255)
                                                                                                              aTTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCa                                                                                                                                                                                                                             <  1:691972/153‑1 (MQ=255)
                                                                                                                   aTCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGa                                                                                                                      <  1:21401/251‑1 (MQ=255)
                                                                                                                   aTCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGa                                                                                                                      <  1:556168/251‑1 (MQ=255)
                                                                                                                   aTCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGa                                                                                                                      <  1:751193/251‑1 (MQ=255)
                                                                                                                        ggCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGa                                                                                                                      <  1:264212/246‑1 (MQ=255)
                                                                                                                               cAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGc                                                                                                          <  1:321486/251‑1 (MQ=255)
                                                                                                                                   tCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTAt                                                                                                       >  1:520947/1‑250 (MQ=255)
                                                                                                                                         aTCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGat                                                                                                <  1:336499/251‑1 (MQ=255)
                                                                                                                                            tGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGataat                                                                                             <  1:605766/251‑1 (MQ=255)
                                                                                                                                             gTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATtat                                                                                                                                                                            >  1:96932/1‑171 (MQ=255)
                                                                                                                                             gTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGa                                                                                                                      >  1:876365/1‑225 (MQ=255)
                                                                                                                                              tttATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGATAatat                                                                                           <  1:387344/251‑1 (MQ=255)
                                                                                                                                                     gCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGATAatatcgc                                                                                        >  1:501454/1‑244 (MQ=255)
                                                                                                                                                        tGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATTTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATta                                                                                                                                                                             <  1:193333/159‑1 (MQ=255)
                                                                                                                                                            tATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGATAATATCGCCTGTAAAATTc                                                                             <  1:290769/251‑1 (MQ=255)
                                                                                                                                                                     caaTATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCGGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATgg                                                                                                                                  <  1:561691/189‑1 (MQ=255)
                                                                                                                                                                     caaTATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCt                                                                                                                                       <  1:809451/184‑1 (MQ=255)
                                                                                                                                                                       atatTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGATAatat                                                                                           <  1:411796/226‑1 (MQ=255)
                                                                                                                                                                       atatTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGATAATATCGCCTGTAAAATTCTATATGcacc                                                                   >  1:401896/1‑247 (MQ=255)
                                                                                                                                                                            gacgaACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGat                                                                                                >  1:275605/4‑216 (MQ=255)
                                                                                                                                                                                  cTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGcc                                                                                                                                        >  1:245499/1‑170 (MQ=255)
                                                                                                                                                                                   tACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGATAATATCGCCTGTAAAATTCTATATGCACCCGACAACCaggag                                                      <  1:797333/251‑1 (MQ=255)
                                                                                                                                                                                   tACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGATAATATCGCCTGTAAAATTCTATATGCACCCGACAACCaggag                                                      <  1:74341/251‑1 (MQ=255)
                                                                                                                                                                                    aCCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATaaa                                                                                                                                                                                                                        <  1:338703/88‑1 (MQ=255)
                                                                                                                                                                                        gAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGATAATATCGCCTGTAAAATTCTATATGCACCCGACAACCAggaggacg                                                  >  1:581203/1‑248 (MQ=255)
                                                                                                                                                                                          aaccaaccCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATgg                                                                                                                                  >  1:868593/1‑168 (MQ=255)
                                                                                                                                                                                                    acaTTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGc                                                                                                                                                                                       >  1:175587/3‑105 (MQ=255)
                                                                                                                                                                                                         aagatatCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTc                                                                                                                                                     >  1:239604/4‑134 (MQ=255)
                                                                                                                                                                                                          aaaTATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGATAATATCGCCTGTAAAATTCTATATGCACCCGACAACCAGGAGGACGCAGAAGAATATTCTAAAGt                               >  1:324203/1‑251 (MQ=255)
                                                                                                                                                                                                          aaaTATCAGTTAATACTACTAATGGATGAGTTTACTACCCTCGATATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCAATAAGCCTAATGGTTACGGTAAAGa                                                                                                                      <  1:278555/164‑1 (MQ=255)
                                                                                                                                                                                                            atatCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGat                                                                                                >  1:735325/1‑184 (MQ=255)
                                                                                                                                                                                                                cAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGATAATATCGCCTGTAAAATTCTATATGCACCCGACAACCAGGAGGACGCAGAAGAATATTCTAAAGTGCTAgg                         >  1:281098/1‑251 (MQ=255)
                                                                                                                                                                                                                  gTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGATAATATCGCCTGTAAAATTCTATATGCACCCGACAACCAGGAGGACGCAGAAGAATATTCTAAAGTGCTAGGTa                       >  1:509854/1‑251 (MQ=255)
                                                                                                                                                                                                                   ttattaCTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGATAATATCGCCTGTAAAATTCTATATGCACCCGACAACCAGGAGGACGCAGAAGAATATTCTAAAGTGCTAGGt                        <  1:601565/249‑1 (MQ=255)
                                                                                                                                                                                                                   ttattaCTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGATAATATCGCCTGTAAAATTCTATATGCACCCGACAACCAGGAGGACGCAGAAGAATATTCTAAAGTGCTAGGTa                       <  1:264366/250‑1 (MQ=255)
                                                                                                                                                                                                                   ttattaCTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACGTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGATAATATCGCCTGTAAAATTCTATAGGCCCCCGACAACCAGGAGGACGCAGAAGAATATTCTAAAGTGCTAGGt                        <  1:491335/249‑1 (MQ=255)
                                                                                                                                                                                                                    tattaCTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGat                                                                                                <  1:75807/176‑1 (MQ=255)
                                                                                                                                                                                                                     attaCTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGATAATATCGCCTGTAAAATTCTATATGCACCCGACAACCAGGAGGACGCAGAAGAATATTCt                                    <  1:522706/235‑1 (MQ=255)
                                                                                                                                                                                                                       tactacTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGATAATATCGCCTGTAAAATTCTATATGCACCCGACAACCAGGAGGACGCAGAAGAATATTc                                     <  1:502246/232‑1 (MQ=255)
                                                                                                                                                                                                                         ctactaATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAggg                                                                                                                                                            >  1:860830/1‑111 (MQ=255)
                                                                                                                                                                                                                         ctactaATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGa                                                                                                                      <  1:399882/149‑1 (MQ=255)
                                                                                                                                                                                                                         ctactaATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGATAATATCGCCTGTAAAATTCTATATGCACCCGACAACCAGGAGGACGCAGAAGAATATTCTAAAGTGCTAGGTAATCGCAc                <  1:153221/251‑1 (MQ=255)
                                                                                                                                                                                                                         ctactaATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGATAATATCGCCTGTAAAATTCTATATGCACCCGACAACCAGGAGGACGCAGAAGAATATTCTAAAGTGCTAGGTAATCGCAc                <  1:597058/251‑1 (MQ=255)
                                                                                                                                                                                                                              aaTGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGATAATATCGCCTGTAAAATTCTATATGCACCCGACAACCAGGAGGACGCAGAAGAatat                                       >  1:118976/2‑223 (MQ=255)
                                                                                                                                                                                                                                   aTGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGATAATATCGCCTGTAAAATTCTATATGCACCCGACAACCAGGAGGACGCAGAAGAATATTCTAAAGTGCTAGGTAATCGCACTGtaaataaa      <  1:871369/251‑1 (MQ=255)
                                                                                                                                                                                                                                    tGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGATGCTAAAGCTATCCTGGATAATATCGCCTGTAAAATTCTATATGCACCCGACAACCAGGAGGACGCAGAAGAATATTCTAAAGTGCTAGGTAATCGCACTGTaaataaat     <  1:663503/251‑1 (MQ=255)
                                                                                                                                                                                                                                       gTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGATAATATCGCCTGTAAAATTCTATATGCACCCGACAACCAGGAGGACGCAGAAGAATATTCTAAAGTGCTAGGTAATCGCACTGTAAATAAATTaa  >  1:728261/1‑251 (MQ=255)
                                                                                                                                                                                                                                       gTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGATAATATCGCCTGTAAAATTCTATATGCACCCGACAACCAGGAGGACGCAGAAGAATATTCTAAAGTGCTAGGTAATCGCACTGTAAATAAATTaa  >  1:834656/1‑251 (MQ=255)
                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                 
AACCATTTTTCAAAATGAAATTGTTGAACATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGATGAACTACCGGAAACCAACCCGAAATTAAAATATCAGTTATTACTACTCATGGATGAGTTTACTGCGGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGCTATGGTTTACGCCTATTGATTATCTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGATAATATTGCCTGTAAAATTCTGTATGCTCCCGACAATCAGGAGGATGCAGAAGAATATTCTAAAGTGCTAGGTAATCGCACTGTAGATAAATTAA  >  1/777348‑777829

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑