Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	78,960	C→T	intergenic (–/+54981)	– / ← unknown	–/tRNA‑Ala‑GGC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	78,960	0	C	T	100.0%	159.3 / NA	47	intergenic (–/+54981)	–/unknown	–/tRNA‑Ala‑GGC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (17/30);  total (17/30)

ATTACAAGACAACCCGCAACGCAACAGCCGCGAAGTGAAAAAACTCACCGCGCAACACATTGAAACCTTAAACGACCAAATACAGGCGTTACAAAAAATGGTCACCGAACTGCAACGTTGGCACGACAACTGCCAAGGTAACGACTGCCCGGAATGCTCGATTTTGGAGGGCTTGAGTGAGTGAAAAGTGCGGTGATTTTTCATTGCAATTTTAAGTAGAAATATAACTAAAAAATCTCTCACGCTTATCTTTTAAACATGCACAATTCCTATAAAATAGGTATATTTTTTTATTTACTCACTTTACAAAGGACAAATTTTATGCAGAAGCCAGAAGCCAGAAGCCAGAAGCCACCAAGATTATGTTGAAAATTCAGCCACACAACAAGCCTTCCTAAACGACCTCGACGAAAAACTTTGGTCTTCCGCCGACCGTCTCCGCCAGCAACTCGACGCCGCCAACTACAAACACATTG  >  1/78718‑79193                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                          aTTACAAGACAACCCGCAACGCAACAGCCGCGAAGTGAAAAAACTCACCGCGCAACACATTGAAACCTTAAACGACCAAATACAGGCGTTACAAAAAATGGTCACCGAACTGCAACGTTGGCACGACAACTGCCAAGGTAACGACTGCCCGGAATGCTCGATTTTGGAGGGCTTGAGTGAGTGAAAAGTGCGGTGATTTTTCATTGCAATTTTAAGTAGAAATATAATTAAAAAATCTCTCATGCTTAt                                                                                                                                                                                                                                     >  1:867525/1‑249 (MQ=255)  ttACAAGACAACCCGCAACGCAACAGCCGCGAAGTGAAAAAACTCACCGCGCAACACATTGAAACCTTAAACGACCAAATACAGGCGTTACAAAAAATGGTCACCGAACTGCAACGTTGGCACGACAACTGCCAAGGTAACGACTGCCCGGAATGCTCGATTTTGGAGGGCTTGAGTGAGTGAAAAGTGCGGTGATTTTTCATTGCAATTTTAAGTAGAAATATAATTAAAAAATCTCTCATGCTTATCtt                                                                                                                                                                                                                                  >  1:876366/1‑251 (MQ=255)  ttACAAGACAACCCGCAACGCAACAGCCGCGAAGTGAAAAAACTCACCGCGCAACACATTGAAACCTTAAACGACCAAATACAGGCGTTACAAAAAATGGTCACCGAACTGCAACGTTGGCACGACAACTGCCAAGGTAACGACTGCCCGGAATGCTCGATTTTGGAGGGCTTGAGTGAGTGAAAAGTGCGGTGATTTTTCATTGCAATTTTAAGTAGAAATATAATTAAAAAATCTCTCATGCTTATCtt                                                                                                                                                                                                                                  >  1:318394/1‑251 (MQ=255)         aCAACCCGCAACGCAACAGCCGCGAAGTGAAAAAACTCACCGCGCAACACATTGAAACCTTAAACGACCAAATACAGGCGTTACAAAAAATGGTCACCGAACTGCAACGTTGGCACGACAACTGCCAAGGTAACGACTGCCCGGAATGCTCGATTTTGGAGGGCTTGAGTGAGTGAAAAGTGCGGTGATTTTTCATTGCAATTTTAAGTAGAAATATAATTAAAAAATCTCTCATGCTTATCTTTTAAAc                                                                                                                                                                                                                            <  1:391561/250‑1 (MQ=255)         aCAACCCGCAACGCAACAGCCGCGAAGTGAAAAAACTCACCGCGCAACACATTGAAACCTTAAACGACCAAATACAGGCGTTACAAAAAATGGTCACCGAACTGCAACGTTGGCACGACAACTGCCAAGGTAACGACTGCCCGGAATGCTCGATTTTGGAGGGCTTGAGTGAGTGAAAAGTGCGGTGATTTTTCATTGCAATTTTAAGTAGAAATATAATTAAAAAATCTCTCATGCTTATCTTTTAAAc                                                                                                                                                                                                                            <  1:312530/250‑1 (MQ=255)         aCAACCCGCAACGCAACAGCCGCGAAGTGAAAAAACTCACCGCGCAACACATTGAAACCTTAAACGACCAAATACAGGCGTTACAAAAAATGGTCACCGAACTGCAACGTTGGCACGACAACTGCCAAGGTAACGACTGCCCGGAATGCTCGATTTTGGAGGGCTTGAGTGAGTGAAAAGTGCGGTGATTTTTCATTGCAATTTTAAGTAGAAATATAATTAAAAAATCTCTCATGCTTATCTTTTAAACa                                                                                                                                                                                                                           <  1:318061/251‑1 (MQ=255)              ccGCAACGCAACAGCCGCGAAGTGAAAAAACTCACCGCGCAACACATTGAAACCTTAAACGACCAAATACAGGCGTTACAAAAAATGGTCACCGAACTGCAACGTTGGCACGACAACTGCCAAGGTAACGACTGCCCGGAATGCTCGATTTTGGAGGGCTTGAGTGAGTGAAAAGTGCGGTGATTTTTCATTGCAATTTTAAGTAGAAATATAATTAAAAAATCTCTCATGCTTATCTTTTAAACATGcac                                                                                                                                                                                                                      <  1:830057/251‑1 (MQ=255)              ccGCAACGCAACAGCCGCGAAGTGAAAAAACTCACCGCGCAACACATTGAAACCTTAAACGACCAAATACAGGCGTTACAAAAAATGGTCACCGAACTGCAACGTTGGCACGACAACTGCCAAGGTAACGACTGCCCGGAATGCTCGATTTTGGAGGGCTTGAGTGAGTGAAAAGTGCGGTGATTTTTCATTGCAATTTTAAGTAGAAATATAATTAAAAAATCTCTCATGCTTATCTTTTAAACATGcac                                                                                                                                                                                                                      <  1:271480/251‑1 (MQ=255)              ccGCAACGCAACAGCCGCGAAGTGAAAAAACTCACCGCGCAACACATTGAAACCTTAAACGACCAAATACAGGCGTTACAAAAAATGGTCACCGAACTGCAACGTTGGCACGACAACTGCCAAGGTAACGACTGCCCGGAATGCTCGATTTTGGAGGGCTTGAGTGAGTGAAAAGTGCGGTGATTTTTCATTGCAATTTTAAGTAGAAATATAATTAAAAAATCTCTCATGCTTATCTTTTAAACATGcac                                                                                                                                                                                                                      <  1:642345/251‑1 (MQ=255)                    cgcaacAGCCGCGAAGTGAAAAAACTCACCGCGCAACACATTGAAACCTTAAACGACCAAATACAGGCGTTACAAAAAATGGTCACCGAACTGCAACGTTGGCACGACAACTGCCAAGGTAACGACTGCCCGGAATGCTCGATTTTGGAGGGCTTGAGTGAGTGAAAAGTGCGGTGATTTTTCATTGCAATTTTAAGTAGAAATATAATTAAAAAATCTCTCATGCTTATCTTTTAAAc                                                                                                                                                                                                                            >  1:727702/1‑239 (MQ=255)                            ccGCGAAGTGAAAAAACTCACCGCGCAACACATTGAAACCTTAAACGACCAAATACAGGCGTTACAAAAAATGGTCACCGAACTGCAACGTTGGCACGACAACTGCCAAGGTAACGACTGCCCGGAATGCTCGATTTTGGAGGGCTTGAGTGAGTGAAAAGTGCGGTGATTTTTCATTGCAATTTTAAGTAGAAATATAATTAAAAAATCTCTCATGCTTATCTTTTAAACATGCACAATTCCTATaaaa                                                                                                                                                                                                         <  1:788178/250‑1 (MQ=255)                            ccGCGAAGTGAAAAAACTCACCGCGCAACACATTGAAACCTTAAACGACCAAATACAGGCGTTACAAAAAATGGTCACCGAACTGCAACGTTGGCACGACAACTGCCAAGGTAACGACTGCCCGGAATGCTCGATTTTGGAGGGCTTGAGTGAGTGAAAAGTGCGGTGATTTTTCATTGCAATTTTAAGTAGAAATATAATTAAAAAATCTCTCATGCTTATCTTTTAAACATGCACAATTCCTATAAAAt                                                                                                                                                                                                        <  1:702887/251‑1 (MQ=255)                                caaaaaaccggtttcagcgcgCAACACATTGAAACCTTAAACGACCAAATACAGGCGTTACAAAAAATGGTCACCGAACTGCAACGTTGGCACGACAACTGCCAAGGTAACGACTGCCCGGAATGCTCGATTTTGGAGGGCTTGAGTGAGTGAAAAGTGCGGTGATTTTTCATTGCAATTTTAAGTAGAAATATAATTAAAAAATCTCTCATGCTTATCtt                                                                                                                                                                                                                                  >  1:195443/18‑221 (MQ=255)                                   gTGAAAAAACTCACCGCGCAACACATTGAAACCTTAAACGACCAAATACAGGCGTTACAAAAAATGGTCACCGAACTGCAACGTTGGCACGACAACTGCCAAGGTAACGACTGCCCGGAATGCTCGATTTTGGAGGGCTTGAGTGAGTGAAAAGTGCGGTGATTTTTCATTGCAATTTTAAGTAGAAATATAATTAAAAAATCTCTCATGCTTATCTTTTAAACATGcac                                                                                                                                                                                                                      >  1:566808/1‑230 (MQ=255)                                      aaaaaaCTCACCGCGCAACGCATTGAAACCTTAAACGACCAAATACAGGCGTTACAAAAAATGGTCACCGAACTGCAACGTTGGCACGACAACTGCCAAGGTAACGACTGCCCGGAATGCTCGATTTTGGAGGGCTTGAGTGAGTGAAAAGTGCGGTGATTTTTCATTGCAATTTTAAGTAGAAATATAATTAAAAAATCTCTCATGCTTATCTTTTAAACATGCACAATTCCTATAAAATAGGTATAttt                                                                                                                                                                                              <  1:159062/251‑1 (MQ=255)                                           aCTCACCGCGCAACACATTGAAACCTTAAACGACCAAATACAGGCGTTACAAAAAATGGTCACCGAACTGCAACGTTGGCACGACAACTGCCAAGGTAACGACTGCCCGGAATGCTCGATTTTGGAGGGCTTGAGTGAGTGAAAAGTGCGGTGATTTTTCATTGCAATTTTAAGTAGAAATATAATTAAAAAATCTCTCATGCTTATCTTTTAAACATGCACAATTCCTATAAAATAGGTATATTTTttta                                                                                                                                                                                         <  1:259283/251‑1 (MQ=255)                                                ccGCGCAACACATTGAAACCTTAAACGACCAAATACAGGCGTTACAAAAAATGGTCACCGAACTGCAACGTTGGCACGACAACTGCCAAGGTAACGACTGCCCGGAATGCTCGATTTTGGAGGGCTTGAGTGAGTGAAAAGTGCGGTGATTTTTCATTGCAATTTTAAGTAGAAATATAATTAAAAAATCTCTCATGCTTATCTTTTAAACATGcac                                                                                                                                                                                                                      >  1:353906/1‑217 (MQ=255)                                                          caTTGAAACCTTAAACGACCAAATACAGGCGTTACAAAAAATGGTCACCGAACTGCAACGTTGGCACGACAACTGCCAAGGTAACGACTGCCCGGAATGCTCGATTTTGGAGGGCTTGAGTGAGTGAAAAGTGCGGTGATTTTTCATTGCAATTTTAAGTAGAAATATAATTAAAAAATCTCTCATGCTTATCTTTTAAACATGCACAATTCCTATAAAATAGGTATATTTTTTTATTTACTc                                                                                                                                                                                  <  1:503296/243‑1 (MQ=255)                                                                  ccTTAAACGACCAAATACAGGCGTTACAAAAAATGGTCACCGAACTGCAACGTTGGCACGACAACTGCCAAGGTAACGACTGCCCGGAATGCTCGATTTTGGAGGGCTTGAGTGAGTGAAAAGTGCGGTGATTTTTCATTGCAATTTTAAGTAGAAATATAATTAAAAAATCTCTCATGCTTATCTTTTAAACATGCACAATTCCTATAAAATAg                                                                                                                                                                                                      <  1:303609/215‑1 (MQ=255)                                                                     tAAACGACCAAATACAGGCGTTACAAAAAATGGTCACCGAACTGCAACGTTGGCACGACAACTGCCAAGGTAACGACTGCCCGGAATGCTCGATTTTGGAGGGCTTGAGTGAGTGAAAAGTGCGGTGATTTTTCATTGCAATTTTAAGTAGAAATATAATTAAAAAATCTCTCATGCTTATCTTTTAAACATGCACAATTCCTATAAAATAGGTATATTTTTTTATTTACTCACTTTACAAAGGACAAAtt                                                                                                                                                               <  1:493640/251‑1 (MQ=255)                                                                           aCCAAATACAGGCGTTACAAAAAATGGTCACCGAACTGCAACGTTGGCACGACAACTGCCAAGGTAACGACTGCCCGGAATGCTCGATTTTGGAGGGCTTGAGTGAGTGAAAAGTGCGGTGATTTTTCATTGCAATTTTAAGTAGAAATATAATTAAAAAATCTCTCATGCTTATCTTTTAAACATGCACAATTCCTATAAAATAGGTATAtttttt                                                                                                                                                                                           >  1:758634/1‑217 (MQ=255)                                                                              aaaTACAGGCGTTACAAAAAATGGTCACCGAACTGCAACGTTGGCACGACAACTGCCAAGGTAACGACTGCCCGGAATGCTCGATTTTGGAGGGCTTGAGTGAGTGAAAAGTGCGGTGATTTTTCATTGCAATTTTAAGTAGAAATATAATTAAAAAATCTCTCATGCTTATCTTTTAAACATGCACAATTCCTATAAAATAGGTATATTTTTTTATTTACTCACTTTAc                                                                                                                                                                           >  1:363010/1‑230 (MQ=255)                                                                              aaaTACAGGCGTTACAAAAAATGGTCACCGAACTGCAACGTTGGCACGACAACTGCCAAGGTAACGACTGCCCGGAATGCTCGATTTTGGAGGGCTTGAGTGAGTGAAAAGTGCGGTGATTTTTCATTGCAATTTTAAGTAGAAATATAATTAAAAAATCTCTCATGCTTATCTTTTAAACATGCACAATTCCTATAAAATAGGTATATTTTTTTATTTACTCACTTTACAAAGGACAAATTTTATGcaga                                                                                                                                                      <  1:132309‑M2/251‑5 (MQ=255)                                                                               aaTACAGGCGTTACAAAAAATGGTCACCGAACTGCAACGTTGGCACGACAACTGCCAAGGTAACGACTGCCCGGAATGCTCGATTTTGGAGGGCTTGAGTGAGTGAAAAGTGCGGTGATTTTTCATTGCAATTTTAAGTAGAAATATAATTAAAAAATCTCTCATGCTTATCTTTTAAACATGCACAATTCCTATAAAATAGGTATATTTTTTTATTTACTCACTTTACAAAg                                                                                                                                                                       >  1:315106/1‑233 (MQ=255)                                                                               aaTACAGGCGTTACAAAAAATGGTCACCGAACTGCAACGTTGGCACGACAACTGCCAAGGTAACGACTGCCCGGAATGCTCGATTTTGGAGGGCTTGAGTGAGTGAAAAGTGCGGTGATTTTTCATTGCAATTTTAAGTAGAAATATAATTAAAAAATCTCTCATGCTTATCTTTTAAACATGCACAATTCCTATAAAATAGGTATATTTTTTTATTTACTCACTTTACAAAGGACAAATTTTATGcagaa                                                                                                                                                     <  1:393135‑M2/251‑6 (MQ=255)                                                                                aTACAGGCGTTACAAAAAATGGTCACCGAACTGCAACGTTGGCACGACAACTGCCAAGGTAACGACTGCCCGGAATGCTCGATTTTGGAGGGCTTGAGTGAGTGAAAAGTGCGGTGATTTTTCATTGCAATTTTAAGTAGAAATATAATTAAAAAATCTCTCATGCTTATCTTTTAAACATGCACAATTCCTATAAAAt                                                                                                                                                                                                        <  1:614756/199‑1 (MQ=255)                                                                                aTACAGGCGTTACAAAAAATGGTCACCGAACTGCAACGTTGGCACGACAACTGCCAAGGTAACGACTGCCCGGAATGCTCGATTTTGGAGGGCTTGAGTGAGTGAAAAGTGCGGTGATTTTTCATTGCAATTTTAAGTAGAAATATAATTAAAAAATCTCTCATGCTTATCTTTTAAACATGCACAATTCCTATAAAATAGGTATATTTTTTTATTTACTCACTTTACAAAGGACAAATTTTATGcagaag                                                                                                                                                    <  1:452043‑M2/251‑7 (MQ=255)                                                                                      gCGTTACAAAAAATGGTCACCGAACTGCAACGTTGGCACGACAACTGCCAAGGTAACGACTGCCCGGAATGCTCGATTTTGGAGGGCTTGAGTGAGTGAAAAGTGCGGTGATTTTTCATTGCAATTTTAAGTAGAAATATAATTAAAAAATCTCTCATGCTTATCTTTTAAACATGCACAATTc                                                                                                                                                                                                                 >  1:566515/1‑184 (MQ=255)                                                                                           aCAAAAAATGGTCACCGAACTGCAACGTTGGCACGACAACTGCCAAGGTAACGACTGCCCGGAATGCTCGATTTTGGAGGGCTTGAGTGAGTGAAAAGTGCGGTGATTTTTCATTGCAATTTTAAGTAGAAATATAATTAAAAAATCTCTCATGCTTATCTTTTAAACATGCACAATTCCTATAAAAt                                                                                                                                                                                                        <  1:464863/188‑1 (MQ=255)                                                                                                               tGCAACGTTGGCACGACAACTGCCAAGGTAACGACTGCCCGGAATGCTCGATTTTGGAGGGCTTGAGTGAGTGAAAAGTGCGGTGATTTTTCATTGCAATTTTAAGTAGAAATATAATTAAAAAATCTCTCATGCTTATCTTTTAAACATGCACAATTCCTATAAAATAGGTATATTTTTTTATTTACTCACTTTACAAAGGACAAATTTTATGcagaagccagaagccaccaagattatgttgaaaatt                                                                                                                      <  1:605221‑M2/250‑37 (MQ=255)                                                                                                                gCAACGTTGGCACGACAACTGCCAAGGTAACGACTGCCCGGAATGCTCGATTTTGGAGGGCTTGAGTGAGTGAAAAGTGCGGTGATTTTTCATTGCAATTTTAAGTAGAAATATAATTAAAAAATCTCTCATGCTTATCTTTTAAACATGCACAATTCCTATAAAATAGGTATATTTTTTTATTTACTCACTTTACAAAGGACAAATTTTATGcagaagccagaagccaccaagattatgttgaaaattca                                                                                                                    <  1:141309‑M2/251‑39 (MQ=255)                                                                                                                                         ggTAACGACTGCCCGGAATGCTCGATTTTGGAGGGCTTGAGTGAGTGAAAAGTGCGGTGATTTTTCATTGCAATTTTAAGTAGAAATATAATTAAAAAATCTCTCATGCTTATCTTTTAAACATGCACAATTCCTATAAAATAGGTATATTTTTTTATTTACTCACTTTACAAAGGACAAATTTTATGcagaagccagaagccaccaagattatgttgaaaattcagccacacaacaagcc                                                                                                     <  1:337573‑M2/241‑54 (MQ=255)                                                                                                                                         ggTAACGACTGCCCGGAATGCTCGATTTTGGAGGGCTTGAGTGAGTGAAAAGTGCGGTGATTTTTCATTGCAATTTTAAGTAGAAATATAATTAAAAAATCTCTCATGCTTATCTTTTAAACATGCACAATTCCTATAAAATAGGTATATTTTTTTATTTACTCACTTTACAAAGGACAAATTTTATGcagaagccagaagcc                                                                                                                                           >  1:219605‑M2/1‑188 (MQ=255)                                                                                                                                         ggTAACGACTGCCCGGAATGCTCGATTTTGGAGGGCTTGAGTGAGTGAAAAGTGCGGTGATTTTTCATTGCAATTTTAAGTAGAAATATAATTAAAAAATCTCTCATGCTTATCTTTTAAACATGCACAATTACTATAAAATAGGTATAtttt                                                                                                                                                                                             <  1:269093/153‑1 (MQ=255)                                                                                                                                          gTAACGACTGCCCGGAATGCTCGATTTTGGAGGGCTTGAGTGAGTGAAAAGTGCGGTGATTTTTCATTGCAATTTTAAGTCGAAATATAATTAAAAAATCTCTCATGCTTATCTTTTAAACATGCACAATTCCTATAAAATAGGTATATTTTTTTATTTACTCACTTTACAAAGGACAAATTTTATGccgaagccagaagccaccaagattatgttgacaattcagccacacaacaagccttcctaaacga                                                                                          <  1:387328‑M2/251‑65 (MQ=255)                                                                                                                                                            gCTCGATTTTGGAGGGCTTGAGTGAGTGAAAAGTGCGGTGATTTTTCATTGCAATTTTAAGTAGAAATATAATTAAAAAATCTCTCATGCTTATCTTTTAAACATGCACAATTCCTATAAAAt                                                                                                                                                                                                        <  1:488063/123‑1 (MQ=255)                                                                                                                                                                           gCTTGAGTGAGTGAAAAGTGCGGTGATTTTTCATTGCAATTTTAAGTAGAAATATAATTAAAAAATCTCTCATGCTTATCTTTTAAACATGCACAATTCCTATAAAATAGGTATATTTTTTTATTTACTCACTTTACAAAGGACAAATTTTATGcagaagccagaagccaccaagattatgttgaaaattcagccacacaacaagccttcctaaacgacctcgacgaaaaactttggtcttccgccgaccg                                                         <  1:365051‑M2/251‑98 (MQ=255)                                                                                                                                                                                   gagtgaAAAGTGCGGTGATTTTTCATTGCAATTTTAAGTAGAAATATAATTAAAAAATCTCTCATGCTTATCTTTTAAACATGCACAATTCCTATAAAATAGGTATATTTTTTTATTTACTCACTTTACAAAGGACAAATTTTATGcagaagccagaagccaccaagattatgttgaaaattcagccacacaacaagccttcctaaacgacctcgacgaaaaactttggtcttccgtcgaccgtctccgc                                                  <  1:271097‑M2/250‑105 (MQ=255)                                                                                                                                                                                                 gTGATTTTTCATTGCAATTTTAAGTAGAAATATAATTAAAAAATCTCTCATGCTTATCTTTTAAACATGCACAATTCCTATAAAATAGGTATATTTTTTTATTTACTCACTTTACAAAGGACAAATTTTATGcagaagccagaagccaccaagattatgttgaaaattcagccacacaacaagccttcct                                                                                                <  1:389694‑M2/190‑59 (MQ=255)                                                                                                                                                                                                          cATTGCAATTTTAAGTAGAAATATAATTAAAAAATCTCTCATGCTTATCTTTTAAACATGCACAATTCCTATAAAATAGGTATATTTTTTTATTTACTCACTTTACAAAGGACAAATTTTATGcagaagccagaagccaccaagattatgttgaaaattcagccacacaacaagccttcctaaacgacctcgacgaaaaactttggtcttccgccgaccgtctccgccagcaactcgacgccgccaactac                          >  1:770658‑M2/1‑123 (MQ=255)                                                                                                                                                                                                              gCAATTTTAAGTAGAAATATAATTAAAAAATCTCTCATGCTTATCTTTTAAACATGCACAATTCCTATAAAATAGGTATATTTTTTTATTTACTCACTTTACAAAGGACAAATTTTATGcagaagccagaagccaccaagattatgttgaaaattcagccacacaacaagcc                                                                                                     <  1:309300‑M2/172‑54 (MQ=255)                                                                                                                                                                                                                    ttAAGTAGAAATATAATTAAAAAATCTCTCATGCTTATCTTTTAAACATGCACAATTCCTATAAAATAGGTATATTTTTTTATTTACTCACTTTACAAAGGGCAAATTTTATGcagaagccagaagccaccaagattatgttgaaaattcagccacacaacaagccttcctaaacgacctcgacgaaaaactttggtcttccgccgaccgtctccgccagcaactcgacgccgccaactacaaacacattg                >  1:393285‑M2/1‑113 (MQ=255)                                                                                                                                                                                                                      aaGTAGAAATATAATTAAAAAATCTCTCATGCTTATCTTTTAAACATGCACAATTCCTATAAAATAGGTATATTTTTTTATTTACTCACTTTACAAAGGACAAATTTTATGcagaagccagaagccaccaagattatgttgaaaattcagccacacaacaagccttcctaaacgacctcgacgaaaaactttggtcttccgccgaccgtctccgccagcaactcgacgccgccaactacaaacacattgtg              >  1:372546‑M2/1‑111 (MQ=255)                                                                                                                                                                                                                            aaaTATAATTAAAAAATCTCTCATGCTTATCTTTTAAACATGCACAATTCCTATAAAATAGGTATATTTTTTTATTTACTCACTTTACAAAGGACAAATTTTATGcagaagccagaagccaccaagattatgttgaaaattcagccacacaacaagccttcctaaacgacctcgacgaaaaactttggtcttccgccgaccgtctccgccagcaactcgacgccgccaactacaaacacattgtgcttggc        >  1:593252‑M2/1‑105 (MQ=255)                                                                                                                                                                                                                            aaaTATAATTAAAAAATCTCTCATGCTTATCTTTTAAACATGCACAATTCCTATAAAATAGGTATATTTTTTTATTTACTCACTTTACAAAGGACAAATTTTATGcagaagccagaagccaccaagattatgttgaaaattcagccacacaacaagccttcctaaacgacctcgacgaaaaactttggtcttccgccgaccgtctccgccagcaactcgacgccgccaactacaaacacattgtgcttgg         <  1:831175‑M2/250‑146 (MQ=255)                                                                                                                                                                                                                                  aattaaaAAATCTCTCATGCTTATCTTTTAAACATGCACAATTCCTATAAAATAGGTATATTTTTTTATTTACTCACTTTACAAAGGACAAATTTTATGcagaagccagaagccaccaagattatgttgaaaattcagccacacaacaagccttcctaaacgacctcgacgaaaaactttggtcttccgccgaccgtctccgccagcaactcgacgccgccaactacaaacacattgtgcttggcttaatc  >  1:693288‑M2/4‑99 (MQ=255)                                                                                                                                                                                                                                             ctctcATGCTTATCTTTTAAACATGCACAATTCCTATAAAATAGGTATATTTTTTTATTTACTCACTTTACAAAGGACAAATTTTATGcagaagccagaagccaccaagattatgttgaaaattcagccacacaacaagccttcctaaacgacctcgacgaaaaactttggtcttccgccgaccgtctccgccag                                               <  1:80567‑M2/195‑108 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                          ATTACAAGACAACCCGCAACGCAACAGCCGCGAAGTGAAAAAACTCACCGCGCAACACATTGAAACCTTAAACGACCAAATACAGGCGTTACAAAAAATGGTCACCGAACTGCAACGTTGGCACGACAACTGCCAAGGTAACGACTGCCCGGAATGCTCGATTTTGGAGGGCTTGAGTGAGTGAAAAGTGCGGTGATTTTTCATTGCAATTTTAAGTAGAAATATAACTAAAAAATCTCTCACGCTTATCTTTTAAACATGCACAATTCCTATAAAATAGGTATATTTTTTTATTTACTCACTTTACAAAGGACAAATTTTATGCAGAAGCCAGAAGCCAGAAGCCAGAAGCCACCAAGATTATGTTGAAAATTCAGCCACACAACAAGCCTTCCTAAACGACCTCGACGAAAAACTTTGGTCTTCCGCCGACCGTCTCCGCCAGCAACTCGACGCCGCCAACTACAAACACATTG  >  1/78718‑79193

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑