Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	474,201	C→T	intergenic (‑35/+40)	unknown ← / ← unknown	tRNA‑SeC(p)‑TCA/Xylulose kinase (EC 2.7.1.17)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	474,201	0	C	T	100.0%	268.6 / NA	75	intergenic (‑35/+40)	unknown/unknown	tRNA‑SeC(p)‑TCA/Xylulose kinase (EC 2.7.1.17)
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (32/43);  total (32/43)

AACCATGCGATGGCTTCTATCGGGTGACAACGCTGCAACCGAAGCTGATAGTCGGCAATCAATTTCGGTCAAAAGTGCGGTCATTTTATGAAGTGTTTTTTAAAAATAAAATTGAGTGGCGGAAGATCGTAGGAGTTGAACCTACCCGAGACTGCTGGCAGCCTCAACAGGATTTGAAGTCCTGCCGCCTCACCGGAAACGACGATCTTCCTCTTGGAAATTGATGCGAACTTTAGCGCAAAAGCCCGTGCGATTCAAGTGAAGCGGAAAAATAATCCATTAATTTTATACGGAATATTGGGCGTTTAAATGTTGGCGATAGCGTTGGTAAACGGATTGATATTGTTCCGCATTTTCCGCTATCGGCGCTGTGCGCGTCGCTTCATTTAACTGTACGAAAGCTCGGCATAATTCCGCCAGCGAACCCTGTTTATTTGCCCACATGGCTTGAATTGCGGCGCCCAAT  >  1/473956‑474421                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                             aaCCATGCGATGGCTTCTATCGGGTGACAACGCTGCAACCGAAGCTGATAGTCGGCAATCAATTTCGGTCAAAAGTGCGGTCATTTTATGAAGTGTTTTTTAAAAATAAAATTGAGTGGCGGAAGATCGTAGGAGTTGAACCTACCCGAGACTGCTGGCAGCCTCAACAGGATTTGAAGTCCTGCCGCCTCACCGGAAACGACGATCTTCCTCTTGGAAATTGATGCGAACTTTAGCGCAAAAGCTCGTGc                                                                                                                                                                                                                         <  1:712125/251‑1 (MQ=255)  aCCATGCGATGGCTTCTATCGGGTGACAACGCTGCAACCGAAGCTGATAGTCGGCAATCAATTTCGGTCAAAAGTGCGGTCATTTTATGAAGTGTTTTTTAAAAATAAAATTGAGTGGCGGAAGATCGTAGGAGTTGAACCTACCCGAGACTGCTGGCAGCCTCAACAGGATTTGAAGTCCTGCCGCCTCACCGGAAACGACGATCTTCCTCTTGGAAATTGATGCGAACTTTAACGCAAAAGCTCGTGCg                                                                                                                                                                                                                        >  1:492526/1‑251 (MQ=255)   ccATGCGATGGCTTCTATCGGGTGACAACGCTGCAACCGAAGCTGATAGTCGGCAATCAATTTCGGTCAAAAGTGCGGTCATTTTATGAAGTGTTTTTTAAAAATAAAATTGAGTGGCGGAAGATCGTAGGAGTTGAACCTACCCGAGACTGCTGGCAGCCTCAACAGGATTTGAAGTCCTGCCGCCTCACCGGAAACGACGATCTTCCTCTTGGAAATTGATGCGAACTTTAGCGCAAAAGCTCGTGCGa                                                                                                                                                                                                                       >  1:42914/1‑251 (MQ=255)      tGCGATGGCTTCTATCGGGTGACAACGCTGCAACCGAAGCTGATAGTCGGCAATCAATTTCGGTCAAAAGTGCGGTCATTTTATGAAGTGTTTTTTAAAAATAAAATTGAGTGGCGGAAGATCGTAGGAGTTGAACCTACCCGAGACTGCTGGCAGCCTCAACAGGATTTGAAGTCCTGCCGCCTCACCGGAAACGACGATCTTCCTCTTGGAAATTGATGCGAACTTTAGCGCAAAAGCTCGTGCGATTc                                                                                                                                                                                                                    <  1:139282/251‑1 (MQ=255)             gCTTCTATCGGGTGACAACGCTGCAACCGAAGCTGATAGTCGGCAATCAATTTCGGTCAAAAGTGCGGTCATTTTATGAAGTGTTTTTTAAAAATAAAATTGAGTGGCGGAAGATCGTAGGAGTTGAACCTACCCGAGACTGCTGGCAGCCTCAACAGGATTTGAAGTCCTGCCGCCTCACCGGAAACGACGATCTTCCTCTTGGAAATTGATGCGAACTTTAGCGCAAAAGCTCGTGCGATTCAAGTg                                                                                                                                                                                                               <  1:404451/249‑1 (MQ=255)                         tGACAACGCTGCAACCGAAGCTGATAGTCGGCAATCAATTTCGGTCAAAAGTGCGGTCATTTTATGAAGTGTTTTTTAAAAATAAAATTGAGTGGCGGAAGATCGTAGGAGTTGAACCTACCCGAGACTGCTGGCAGCCTCAACAGGATTTGAAGTCCTGCCGCCTCACCGGAAACGACGATCTTCCTCTTGGAAATTGATGCGAACTTTAGCGCAAAAGCTCGTGCGATTCAAGTGAAGCGGAAAaataa                                                                                                                                                                                                 >  1:585517/1‑251 (MQ=255)                                 cTGCAACCGAAGCTGATAGTCGGCAATCAATTTCGGTCAAAAGTGCGGTCATTTTATGAAGTGTTTTTTAAAAATAAAATTGAGTGGCGGAAGATCGTAGGAGTTGAACCTACCCGAGACTGCTGGCAGCCTCAACAGGATTTGAAGTCCTGCCGCCTCACCGGAAACGACGATCTTCCTCTTGGAAATTGATGCGAACTTTAGCGCAAAAGCTCGTGCGATTCAAGTGAAGCGGAAAAATAATCCATTaa                                                                                                                                                                                         >  1:753560/1‑251 (MQ=255)                                          aaGCTGATAGTCGGCAATCAATTTCGGTCAAAAGTGCGGTCATTTTATGAAGTGTTTTTTAAAAATAAAATTGAGTGGCGGAAGATCGTAGGAGTTGAACCTACCCGAGACTGCTGGCAGCCTCAACAGGATTTGAAGTCCTGCCGCCTCACCGGAAACGACGATCTTCCTCTTGGAAATTGATGCGAACTTTAGCGCAAAAGCTCGTGCGATTCAAGTGAAGCGGAAAAATAATCCATTAATTTTATAc                                                                                                                                                                                 <  1:684963/250‑1 (MQ=255)                                           aGCTGATAGTCGGCAATCAATTTCGGTCAAAAGTGCGGTCATTTTATGAAGTGTTTTTTAAAAATAAAATTGAGTGGCGGAAGATCGTAGGAGTTGAACCTACCCGAGACTGCTGGCAGCCTCAACAGGATTTGAAGTCCTGCCGCCTCACCGGAAACGACGATCTTCCTCTTGGAAATTGATGCGAACTTTAGCGCAAAAGCTCGTGCGATTCAAGTGAAGCGGAAAAATAATCCATTAATTTTATACgg                                                                                                                                                                               >  1:700695/1‑251 (MQ=255)                                               gATAGTCGGCAATCAATTTCGGTCAAAAGTGCGGTCATTTTATGAAGTGTTTTTTAAAAATAAAATTGAGTGGCGGAAGATCGTAGGAGTTGAACCTACCCGAGACTGCTGGCAGCCTCAACAGGATTTGAAGTCCTGCCGCCTCACCGGAAACGACGATCTTCCTCTTGGAAATTGATGCGAACTTTAGCGCAAAAGCTCGTGCGATTCAAGTGAAGCGGAAAAATAATCCATTAATTTTATACGGAata                                                                                                                                                                           >  1:31464/1‑251 (MQ=255)                                                    tCGGCAATCAATTTCGGTCAAAAGTGCGGTCATTTTATGAAGTGTTTTTTAAAAATAAAATTGAGTGGCGGAAGGTCGTAGGAGTTGACCCTACCCGAGACTGCTGGCAGCCTCAACAGGATTTGAAGTCCTGCCGCCTCAACGGAAACGACGATCTTGCTCTTGGAAATTGATGCGAACTTTAGCGCAAAAGCTCGTGCGATTCAAGTGAAGCGGAAAAATAATCCATTAATTTTATACGGAATATTggg                                                                                                                                                                      <  1:584498/251‑1 (MQ=255)                                                    tCGGCAATCAATTTCGGTCAAAAGTGCGGTCATTTTATGAAGTGTTTTTTAAAAATAAAATTGAGTGGCGGAAGATCGTAGGAGTTGAACCTACCCGAGACTGCTGGCAGCCTCAACAGGATTTGAAGTCCTGCCGCCTCACCGGAAACGACGATCTTCCTCTTGGAAATTGATGCGAACTTTAGCGCAAAAGCTCGTGCGATTCAAGTGAAGCGGAAAAATAATCCATTAATTTTATACGGAATATTggg                                                                                                                                                                      <  1:728588/251‑1 (MQ=255)                                                    tCGGCAATCAATTTCGGTCAAAAGTGCGGTCATTTTATGAAGTGTTTTTTAAAAATAAAATTGAGTGGCGGAAGATCGTAGGAGTTGAACCTACCCGAGACTGCTGGCAGCCTCAACAGGATTTGAAGTCCTGCCGCCTCACCGGAAACGACGATCTTCCTCTTGGAAATTGATGCGAACTTTAGCGCAAAAGCTCGTGCGATTCAAGTGAAGCGGAAAAATAATCCATTAATTTTATACGGAATATTggg                                                                                                                                                                      <  1:636778/251‑1 (MQ=255)                                                    tCGGCAATCAATTTCGGTCAAAAGTGCGGTCATTTTATGAAGTGTTTTTTAAAAATAAAATTGAGTGGCGGAAGATCGTAGGAGTTGAACCTACCCGAGACTGCTGGCAGCCTCAACAGGATTTGAAGTCCTGCCGCCTCACCGGAAACGACGATCTTCCTCTTGGAAATTGATGCGAACTTTAGCGCAAAAGCTCGTGCGATTCAAGTGAAGCGGAAAAATAATCCATTAATTTTATACGGAATATTggg                                                                                                                                                                      <  1:605673/251‑1 (MQ=255)                                                    tCGGCAATCAATTTCGGTCAAAAGTGCGGTCATTTTATGAAGTGTTTTTTAAAAATAAAATTGAGTGGCGGAAGATCGTAGGAGTTGAACCTACCCGAGACTGCTGGCAGCCTCAACAGGATTTGAAGTCCTGCCGCCTCACCGGAAACGACGATCTTCCTCTTGGAAATTGATGCGAACTTTAGCGCAAAAGCTCGTGCGATTCAAGAGAAGCGGAAAAATAATCCATTAATTTTATACGGAATATTggg                                                                                                                                                                      <  1:48180/251‑1 (MQ=255)                                                     cGGCAATCAATTTCGGTCAAAAGTGCGGTCATTTTATGAAGTGTTTTTTAAAAATAAAATTGAGTGGCGGAAGATCGTAGGAGTTGAACCTACCCGAGACTGCTGGCAGCCTCAACAGGATTTGAAGTCCTGCCGCCTCACCGGAAACGACGATCTTCCTCTTGGAAATTGATGCGAACTTTAGCGCAAAAGCTCGTGCGATTCAAGTGAAGCGGAAAAATAATCCATTAATTTTATACGGAATATTGGGc                                                                                                                                                                     <  1:523930/251‑1 (MQ=255)                                                       gCAATCAATTTCGGTCAAAAGTGCGGTCATTTTATGAAGTGTTTTTTAAAAATAAAATTGAGTGGCGGAAGATCGTAGGAGTTGAACCTACCCGAGACTGCTGGCAGCCTCAACAGGATTTGAAGTCCTGCCGCCTCACCGGAAACGACGATCTTCCTCTTGGAAATTGATGCGAACTTTAGCGCAAAAGCTCGTGCGATTCAAGTGAAGCGGAAAAATAATCCATTAATTTTATACGGAATATTGGGCg                                                                                                                                                                    <  1:642645/250‑1 (MQ=255)                                                               tttCGGTCAAAAGTGCGGTCATTTTATGAAGTGTTTTTTAAAAATAAAATTGAGTGGCGGAAGATCGTAGGAGTTGAACCTACCCGAGACTGCTGGCAGCCTCAACAGGATTTGAAGTCCTGCCGCCTCACCGGAAACGACGATCTTCCTCTTGGAAATTGATGCGAACTTTAGCGCAAAAGCTCGTGCGATTCAAGTGAAGCGGAAAAATAATCCATTAATTTTATACGGAATATTGGGCGTTTAAATGt                                                                                                                                                           <  1:470098/251‑1 (MQ=255)                                                                  cGGTCAAAAGTGCGGTCATTTTATGAAGTGTTTTTTAAAAATAAAATTGAGTGGCGGAAGATCGTAGGAGTTGAACCTACCCGAGACTGCTGGCAGCCTCAACAGGATTTGAAGTCCTGCCGCCTCACCGGAAACGACGATCTTCCTCTTGGAAATTGATGCGAACTTTAGCGCAAAAGCTCGTGCGATTCAAGTGAAGCGGAAAAATAATCCATTAATTTTATACGGAATATTGGGCGTTTAAATg                                                                                                                                                            <  1:651223/247‑1 (MQ=255)                                                                   ggTCAAAAGTGCGGTCATTTTATGAAGTGTTTTTTAAAAATAAAATTGAGTGGCGGAAGATCGTAGGAGTTGAACCTACCCGAGACTGCTGGCAGCCTCAACAGGATTTGAAGTCCTGCCGCCTCACCGGAAACGACGATCTTCCTCTTGGAAATTGATGCGAACTTTAGCGCAAAAGCTCGTGCGATTCAAGTGAAGCGGAAAAATAATCCATTAATTTTATACGGAATATTGGGCGTTTAAATGTTgg                                                                                                                                                        >  1:563658/1‑250 (MQ=255)                                                                        aaaGTGCGGTCATTTTATGAAGTGTTTTTTAAAAATAAAATTGAGTGGCGGAAGATCGTAGGAGTTGAACCTACCCGAGACTGCTGGCAGCCTCAACAGGATTTGAAGTCCTGCCGCCTCACCGGAAACGACGATCTTCCTCTTGGAAATTGATGCGAACTTTAGCGCAAAAGCTCGTGCGATTCAAGTGAAGCGGAAAAATAATCCATTAATTTTATACGGAATATTGGGCGTTTAAATGTTGGCGATAg                                                                                                                                                  >  1:164962/1‑251 (MQ=255)                                                                        aaaGTGCGGTCATTTTATGAAGTGTTTTTTAAAAATAAAATTGAGTGGCGGAAGATCGTAGGAGTTGAACCTACCCGAGACTGCTGGCAGCCTCAACAGGATTTGAAGTCCTGCCGCCTCACCGGAAACGACGATCTTCCTCTTGGAAATTGATGCGAACTTTAGCGCAAAAGCTCGTGCGATTCAAGTGAAGCGGAAAAATAATCCATTAATTTTATACGGAATATTGGGCGTTTAAATGTTGGCGATAg                                                                                                                                                  >  1:259682/1‑251 (MQ=255)                                                                           gTGCGGTCATTTTATGAAGTGTTTTTTAAAAATAAAATTGAGTGGCGGAAGATCGTAGGAGTTGAACCTACCCGAGACTGCTGGCAGCCTCAACAGGATTTGAAGTCCTGCCGCCTCACCGGAAACGACGATCTTCCTCTTGGAAATTGATGCGAACTTTAGCGCAAAAGCTCGTGCGATTCAAGTGAAGCGGAAAAATAATCCATTAATTTTATACGGAATATTGGGCGTTTAAATGTTGGCGATAGCGt                                                                                                                                               >  1:236609/1‑251 (MQ=255)                                                                                 tCATTTTATGAAGTGTTTTTTAAAAATAAAATTGAGTGGCGGAAGATCGTAGGAGTTGAACCTACCCGAGACTGCTGGCAGCCTCAACAGGATTTGAAGTCCTGCCGCCTCACCGGAAACGACGATCTTCCTCTTGGAAATTGATGCGAACTTTAGCGCAAAAGCTCGTGCGATTCAAGTGAAGCGGAAAAATAATCCATTAATTTTATACGGAATATTGGGCGTTTAAATGTTGGCGATAGCGTTGGTa                                                                                                                                          <  1:415244/250‑1 (MQ=255)                                                                                 tCATTTTATGAAGTGTTTTTTAAAAATAAAATTGAGTGGCGGAAGATCGTAGGAGTTGAACCTACCCGAGACTGCTGGCAGCCTCAACAGGATTTGAAGTCCTGCCGCCTCACCGGAAACGACGATCTTCCTCTTGGAAATTGATGCGAACTTTAGCGCAAAAGCTCGTGCGATTCAAGTGAAGCGGAAAAATAATCCATTAATTTTATACGGAATATTGGGCGTTTAAATGTTGGCGATAGCGTTGGTa                                                                                                                                          <  1:894716/250‑1 (MQ=255)                                                                                 tCATTTTATGAAGTGTTTTTTAAAAATAAAATTGAGTGGCGGAAGATCGTAGGAGTTGAACCTACCCGAGACTGCTGGCAGCCTCAACAGGATTTGAAGTCCTGCCGCCTCACCGGAAACGACGATCTTCCTCTTGGAAATTGATGCGAACTTTAGCGCAAAAGCTCGTGCGATTCAAGTGAAGCGGAAAAATAATCCATTAATTTTATACGGAATATTGGGCGTTTAAATGTTGGCGATAGCGTTGGTa                                                                                                                                          <  1:499458/250‑1 (MQ=255)                                                                                  cATTTTATGAAGTGTTTTTTAAAAATAAAATTGAGTGGCGGAAGATCGTAGGAGTTGAACCTACCCGAGACTGCTGGCAGCCTCAACAGGATTTGAAGTCCTGCCGCCTCACCGGAAACGACGATCTTCCTCTTGGAAATTGATGCGAACTTTAGCGCAAAAGCTCGTGCGATTCAAGTGAAGCGGAAAAATAATCCATTAATTTTATACGGAATATTGGGc                                                                                                                                                                     <  1:519015/222‑1 (MQ=255)                                                                                    ttttATGAAGTGTTTTTTAAAAATAAAATTGAGTGGCGGAAGATCGTAGGAGTTGAACCTACCCGAGACTGCTGGCAGCCTCAACAGGATTTGAAGTCCTGCCGCCTCACCGGAAACGACGATCTTCCTCTTGGAAATTGATGCGAACTTTAGCGCAAAAGCTCGTGCGATTCAAGTGAAGCGGAAAAATAATCCATTAATTTTATACGGAATATTGGGCGTTTAAATGTTGGCGATAGCGTTGGTAAACg                                                                                                                                      >  1:729302/1‑251 (MQ=255)                                                                                          gAAGTGTTTTTTAAAAATAAAATTGAGTGGCGGAAGATCGTAGGAGTTGAACCTACCCGAGACTGCTGGCAGCCTCAACAGGATTTGAAGTCCTGCCGCCTCACCGGAAACGACGATCTTCCTCTTGGAAATTGATGCGAACTTTAGCGCAAAAGCTCGTGCGATTCAAGTGAAGCGGAAAAATAATCCATTAATTTTATACGGAATATTGGGCGTTTAAATGTTGGCGATAGCGTTGGTAAACGGATTGa                                                                                                                                >  1:66159/1‑251 (MQ=255)                                                                                           aaGTGTTTTTGAAAAATAAAATTGAGTGGCGGAAGATCGTAGGAGTTGAACCTACCCGAGACTGCTGGCAGCCTCAACAGGATTTGAAGTCCTGCCGCCTCACCGGAAACGACGATCTTCCTCTTGGAAATTGATGCGAACTTTAGCGCAAAAGCTCGTGCGATTCAAGTGAAGCGGAAAAATAATCCATTAATTTTATACGGAATATTGGGCGTTTAAATGTTGGCGATAGCGTTGGTAAACGGATTGat                                                                                                                               <  1:793411/251‑1 (MQ=255)                                                                                              tgtTTTTTAAAAATAAAATTGAGTGGCGGAAGATCGTAGGAGTTGAACCTACCCGAGACTGCTGGCAGCCTCAACAGGATTTGAAGTCCTGCCGCCTCACCGGAAACGACGATCTTCCTCTTGGAAATTGATGCGAACTTTAGCGCAAAAGCTCGTGCGATTCAAGTGAAGCGGAAAAATAATCCATTAATTTTATACGGAATATTGGGCGTTTAAATGTTGGCGATAGCGTTGGTAAACGGATTGatat                                                                                                                             <  1:235263/250‑1 (MQ=255)                                                                                              tgtTTTTTAAAAATAAAATTGAGTGGCGGAAGATCGTAGGAGTTGAACCTACCCGAGACTGCTGGCAGCCTCAACAGGAGTTGAAGTCCTGCCGCCTCACCGGAAACGACGATCTTCCTCTTGGAAATTGATGCGAACTTTAGCGCAAAAGCTCGTGCGATTCAAGTGAAGCGGAAAAATAATCCATTAATTTTATACGGAATATTGGGCGTTTAAATGTTGGCGATAGCGTTGGTAAACGGATTGata                                                                                                                              <  1:434694/249‑1 (MQ=255)                                                                                                 tttttAAAAATAAAATTGAGTGGCGGAAGATCGTAGGAGTTGAACCTACCCGAGACTGCTGGCAGCCTCAACAGGATTTGAAGTCCTGCCGCCTCACCGGAAACGACGATCTTCCTCTTGGAAATTGATGCGAACTTTAGCGCAAAAGCTCGTGCGATTCAAGTGAAGCGGAAAAATAATCCATTAATTTTATACGGAATATTGGGCGTTTAAATGTTGGCGATAGCGTTGGTAAACGGATTGATATTGtt                                                                                                                         >  1:263571/1‑251 (MQ=255)                                                                                                  ttttAAAAATAAAATTGAGTGGCGGAAGATCGTAGGAGTTGAACCTACCCGAGACTGCTGGCAGCCTCAACAGGATTTGAAGTCCTGCCGCCTCACCGGAAACGACGATCTTCCTCTTGGAAATTGATGCGAACTTTAGCGCAAAAGCTCGTGCGATTCAAGTGAAGCGGAAAAATAATCCATTAATTTTATACGGAATATTGGGCGTTTAAATGTTGGCGATAGCGTTGGTAAACGGATTGATATTGTTc                                                                                                                        <  1:142991/251‑1 (MQ=255)                                                                                                     tAAAAATAAAATTGAGTGGCGGAAGATCGTAGGAGTTGAACCTACCCGAGACTGCTGGCAGCCTCAACAGGATTTGAAGTCCTGCCGCCTCACCGGAAACGACGATCTTCCTCTTGGAAATTGATGCGAACTTTAGCGCAAAAGCTCGTGCGATTCAAGTGAAGCGGAAAAATAATCCATTAATTTTATACGGAATATTGGGCGTTTAAATGTTGGCGATAGCGTTGGTAAACGGATTGATATTGTTCCGc                                                                                                                     <  1:664745/251‑1 (MQ=255)                                                                                                        aaataaaatTGAGTGGCGGAAGATCGTAGGAGTTGAACCTACCCGAGACTGCTGGCAGCCTCAACAGGATTTGAAGTCCTGCCGCCTCACCGGAAACGACGATCTTCCTCTTGGAAATTGATGCGAACTTTAGCGCAAAAGCTCGTGCGATTCAAGTGAAGCGGAAAAATAATCCATTAATTTTATACGGAATATTggg                                                                                                                                                                      >  1:497453/1‑199 (MQ=255)                                                                                                        aaataaaatTGAGTGGCGGAAGATCGTAGGAGTTGAACCTACCCGAGACTGCTGGCAGCCTCAACAGGATTTGAAGTCCTGCCGCCTCACCGGAAACGACGATCTTCCTCTTGGAAATTGATGCGAACTTTAGCGCAAAAGCTCGTGCGATTCAAGTGAAGCGGAAAAATAATCCATTAATTTTATACGGAATATTGGGCGTTTAAATGTTGGCGATAGCGTTGGTAAACGGATTGATATTGTTCCGCAtt                                                                                                                  >  1:62091/1‑251 (MQ=255)                                                                                                             aaatTGAGTGGCGGAAGATCGTAGGAGTTGAACCTACCCGAGACTGCTGGCAGCCTCAACAGGATTTGAAGTCCTGCCGCCTCACCGGAAACGACGATCTTCCTCTTGGAAATTGATGCGAACTTTAGCGCAAAAGCTCGTGCGATTCAAGTGAGGCGGAAAAATAATCCATTAAttt                                                                                                                                                                                      <  1:531704/178‑1 (MQ=255)                                                                                                                      ggCGGAAGATCGTAGGAGTTGAACCTACCCGAGACTGCTGGCAGCCTCAACAGGATTTGAAGTCCTGCCGCCTCACCGGAAACGACGATCTTCCTCTTGGAAATTGATGCGAACTTTAGCGCAAAAGCTCGTGCGATTCAAGTGAAGCGGAAAAATAATCCATTAATTTTATACGGAATATTGGGCGTTTAAATGTTGGCGAt                                                                                                                                                    <  1:399509/203‑1 (MQ=255)                                                                                                                      ggCGGAAGATCGTAGGAGTTGAACCTACCCGAGACTGCTGGCAGCCTCAACAGGATTTGAAGTCCTGCCGCCTCACCGGAAACGACGATCTTCCTCTTGGAAATTGATGCGAACTTTAGCGCAAAAGCTCGTGCGATTCAAGTGAAGCGGAAAAATAATCCATTAATTTTATACGGAATATTGGGCGTTTAAATGTTGGCGATAGCGTTGGTAAACGGATTGATATTGTTCCGCATTTTCCGCTATCGgcg                                                                                                    >  1:464055/1‑251 (MQ=255)                                                                                                                       gCGGAAGATCGTAGGAGTTGAACCTACCCGAGACTGCTGGCAGCCTCAACAGGATTTGAAGTCCTGCCGCCTCACCGGAAACGACGATCTTCCTCTTGGAAATTGATGCGAACTTTAGCGCAAAAGCTCGTGCGATTCAAGTGAAGCGGAAAAATAATCCATTAATTTTATACGGAATATTGGGCGTTTAAATGTTGGCGATAGCGTTGGTAAACGGATTGATATTGTTCCGCATTTTCCGCTATCGgcg                                                                                                    >  1:752104/1‑250 (MQ=255)                                                                                                                          gAAGATCGTAGGAGTTGAACCTACCCGAGACTGCTGGCAGCCTCAACAGGATTTGAAGTCCTGCCGCCTCACCGGAAACGACGATCTTCCTCTTGGAAATTGATGCGAACTTTAGCGCAAAAGCTCGTGCGATTCAAGTGAAGCGGAAAAATAATCCATTAATTTTATACGGAATATTGGGCGTTTAAATGTTGGCGATAGCGTTGGTAAAc                                                                                                                                       >  1:699510/1‑212 (MQ=255)                                                                                                                             gATCGTAGGAGTTGAACCTACCCGAGACTGCTGGCAGCCTCAACAGGATTTGAAGTCCTGCCGCCTCACCGGAAACGACGATCTTCCTCTTGGAAATTGATGCGAACTTTAGCGCAAAAGCTCGTGCGATTCAAGTGAAGCGGAAAAATAATCCATTAATTTTATACGGAATATTggg                                                                                                                                                                      >  1:896063/1‑178 (MQ=255)                                                                                                                             gATCGTAGGAGTTGAACCTACCCGAGACTGCTGGCAGCCTCAACAGGATTTGAAGTCCTGCCGCCTCACCGGAAACGACGATCTTCCTCTTGGAAATTGATGCGAACTTTAGCGCAAAAGCTCGTGCGATTCAAGTGAAGCGGAAAAATAATCCATTAATTTTATACGGAATATTGGGCGTTTAAATGTTGGCGATAGCGTTGGTAAACGGATTGATATTGTTCCGCATTTTCCGCTATCGGCGCTGTgcg                                                                                             >  1:596085/1‑251 (MQ=255)                                                                                                                              aTCGTAGGAGTTGAACCTACCCGAGACTGCTGGCAGCATCAACAGGATTTGAAGTCCTGCCGCCTCACCGGAAACGACGATCTTCCTCTTGGAAATTGATGCGAACTTTAGCGCAACAGCTCGTGCGATTCAAGTGAAGCGGAAAAATAATCCATTAATTCTATATGGTATATTGGGCGTTTAAATGTTGGCGATAGCGCTGGT‑AACGGATTGATATTGTTCCGCATTTTCCGCTATCGGCGCTGTGcgcg                                                                                           >  1:415597/1‑251 (MQ=255)                                                                                                                               tCGTAGGAGTTGAACCTACCCGAGACTGCTGGCAGCCTCAACAGGATTTGAAGTCCTGCCGCCTCACCGGAAACGACGATCTTCCTCTTGGAAATTGATGCGAACTTTAGCGCAAAAGCTCGTGCGATTCAAGTGAAGCGGAAAAATAATc                                                                                                                                                                                               <  1:349248/151‑1 (MQ=255)                                                                                                                               tCGTAGGAGTTGAACCTACCCGAGACTGCTGGCAGCCTCAACAGGATTTGAAGTCCTGCCGCCTCACCGGAAACGACGATCTTCCTCTTGGAAATTGATGCGAACTTTAGCGCAAAAGCTCGTGCGATTCAAGTGAAGCGGAAAAATAATCCATTAATTTTATAc                                                                                                                                                                                 <  1:319168/165‑1 (MQ=255)                                                                                                                               tCGTAGGAGTTGAACCTACCCGAGACTGCTGGCAGCCTCAACAGGATTTGAAGTCCTGCCGCCTCACCGGAAACGACGATCTTCCTCTTGGAAATTGATGCGAACTTTAGCGCAAAAGCTCGTGCGATTCAAGTGAAGCGGAAAAATAATCCATTAATTTTATACGGAATATTGGGCGTTTAAATGTTGGCGATAGCGTTGGTAAACGGATTGATATTGTTCCGCATTTTCCGCTATCGGCGCTGTGcgcg                                                                                           >  1:398042/1‑251 (MQ=255)                                                                                                                                  tAGGAGTTGAACCTACCCGAGACTGCTGGCAGCCTCAACAGGATTTGAAGTCCTGCCGCCTCACCGGAAACGACGATCTTTCTCTTGGAAATTGATGCGAACTTTAGCGCAAAAGCTCGTGCGATTCAAGTGAAGCGGAAAAATAATCCATTAATTTTATACGGAATATTGGGCGTTTAAATGTTGGCGATAGCGTTGGTAAACGGATTGATATTGTTCCGCATTTTCCGCTATCGGCGCTGTGCGCGTc                                                                                         <  1:424206/250‑1 (MQ=255)                                                                                                                                     gAGTTGAACCTACCCGAGACTGCTGGCAGCCTCAACAGGATTTGAAGTCCTGCCGCCTCACCGGAAACGACGATCTTCCTCTTGGAAATTGATGCGAACTTTAGCGCAAAAGCTCGTGCGATTCAAGTGAAGCGGAAAAATAATCCATTAATTTTATACGGAATATTGGGCGTTTAAATGTTGGCGATAGCGTTGGTAAACGGATTGATATTGTTCCGCATTTTCCGCTATCGGCGCTGTGCGCGTCGCtt                                                                                     >  1:288800/1‑251 (MQ=255)                                                                                                                                          gAACCTACCCGAGACTGCTGGCAGCCTCAACAGGATTTGAAGTCCTGCCGCCTCACCGGAAACGACGATCTTCCTCTTGGAAATTGATGCGAACTTTAGCGCAAAAGCTCGTGCGATTCAAGTGAAGCGGAAAAATAATCCATTAATTTTATACGGAATATTGGGCGTTTAAATGTTGGCGATAGCGTTGGTAAACGGATTGatat                                                                                                                             <  1:357923/206‑1 (MQ=255)                                                                                                                                             ccTACCCGAGACTGCTGGCAGCCTCAACAGGATTTGAAGTCCTGCCGCCTCACCGGAAACGACGATCTTCCTCTTGGAAATTGATGCGAACTTTAGCGCAAAAGCTCGTGCGATTCAAGTGAAGCGGAAAAATAATCCATTAATTTTATACGGAATATTGGGCGTTTAAATGTTGGCGATAGCGTTGGTAAACGGATTGATATTGTTCCGCATTTTCCGCTATCGGCGCTGTGCGCGTCGCTTCATTTAAc                                                                             <  1:872038/251‑1 (MQ=255)                                                                                                                                                aCCCGAGACTGCTGGCAGCCTCAACAGGATTTGAAGTCCTGCCGCCTCACCGGAAACGACGATCTTCCTCTTGGAAATTGATGCGAACTTTAGCGCAAAAGCTCGTGCGATTCAAGTGAAGCGGAAAAATAATCCATTAATTTTATACGGAATATTGGGCGTTTAAATg                                                                                                                                                            <  1:826831/169‑1 (MQ=255)                                                                                                                                                aCCCGAGACTGCTGGCAGCCTCAACAGGATTTGAAGTCCTGCCGCCTCACCGGAAACGACGATCTTCCTCTTGGAAATTGATGCGAACTTTAGCGCAAAAGCTCGTGCGATTCAAGTGAAGCGGAAAAATAATCCATTAATTTTATACGGAATATTGGGCGTTTAAATGTTGGCGATAGCGTTGGTAAACGGATTGATATTGTTCCGCATTTTCCGCTATCGGCGCTGTGCGCGTCGCTTCATTTaa                                                                              >  1:714430/1‑247 (MQ=255)                                                                                                                                                aCCCGAGACTGCTGGCAGCCTCAACAGGATTTGAAGTCCTGCCGCCTCACCGGAAACGACGATCTTCCTCTTGGAAATTGATGCGAACTTTAGCGCAAAAGCTCGTGCGATTCAAGTGAAGCGGAAAAATAATCCATTAATTTTATACGGAATATTGGGCGTTTAAATGTTGGCGATAGCGTTGGTAAACGGATTGATATTGTTCCGCATTTTCCGCTATCGGCGCTGTGCGCGTCGCTTCATTTAAc                                                                             <  1:529616/248‑1 (MQ=255)                                                                                                                                                     agaCTGCTGGCAGCCTCAACAGGATTTGAAGTCCTGCCGCCTCACCGGAAACGACGATCTTCCTCTTGGAAATTGATGCGAACTTTAGCGCAAAAGCTCGTGCGATTCAAGTGAAGCGGAAAAATAATCCATTAATTTTATACGGAATATTGGGCGTTTAAATGt                                                                                                                                                           <  1:497269/165‑1 (MQ=255)                                                                                                                                                       aCTGCTGGCAGCCTCAACAGGATTTGAAGTCCTGCCGCCTCACCGGAAACGACGATCTTCCTCTTGGAAATTGATGCGAACTTTAGCGCAAAAGCTCGTGCGATTCAAGTGAAGCGGAAAAATAATCCATTAATTTTATACGGAATATTGGGCGTTTAAATGTTGGCGATAGCGTTGGTAAACGGATTGATATTGTTCCGCATTTTCCGCTATCGGCGCTGTGCGCGTCGCTTCATTTAACTGTACGaa                                                                     <  1:240666/249‑1 (MQ=255)                                                                                                                                                          gctgGCAGCCTCAACAGGATTTGAAGTCCTGCCGCCTCACCGGAAACGACGATCTTCCTCTTGGAAATTGATGCGAACTTTAGCGCAAAAGCTCGTGCGATTCAAGTGAAGCGGAAAAATAATCCATTAATTTTATACGGAATATTGGGCGTTTAAATGTTGGCGATAGCGTTGGTAAACGGATTGATATTGTTCCGCATTTTCCGCTATCGGCGCTGTGCGCGTCGCTTCATTTAAc                                                                             >  1:33361/1‑238 (MQ=255)                                                                                                                                                                   cTCAACAGGATTTGAAGTCCTGCCGCCTCACCGGAAACGACGATCTTCCTCTTGGAAATTGATGCGAACTTTAGCGCAAAAGCTCGTGCGATTCAAGTGAAGCGGAAAAATAATCCATTAATTTTATACGGAATATTGGGCGTTTAAATGTTGGCGATAGCGTTGGTAAACGGATTGATATTGTTCCGCATTTTCCGCTATCGGCGCTGTGCGCGTCGCTTCATTTAAc                                                                             >  1:6931/1‑229 (MQ=255)                                                                                                                                                                           gATTTGAAGTCCTGCCGCCTCACCGGAAACGACGATCTTCCTCTTGGAAATTGATGCGAACTTTAGCGCAAAAGCTCGTGCGATTCAAGTGAAGCGGAAAAATAATCCATTAATTTTATACGGAATATTGGGCGTTTAAATGTTGGCGATAGCGTTGGTAAACGGATTGATATTGTTCCGCATTTTCCGCTATCGGCGCTGTGCGCGTCGCTTCATTTAACTGTACGAAAGCTCGGCATAATTCCGcc                                                  <  1:93082/248‑1 (MQ=255)                                                                                                                                                                             tttGAAGTCCTGCCGCCTCACCGGAAACGACGATCTTCCTCTTGGAAATTGATGCGAACTTTAGCGCAAAAGCTCGTGCGATTCAAGTGAAGCTGAAAAATAATCCATTAATTTTATACGGAATATTGGGCGTTTAAATg                                                                                                                                                            <  1:123512/140‑1 (MQ=255)                                                                                                                                                                             tttGAAGTCCTGCCGCCTCACCGGAAACGACGATCTTCCTCTTGGAAATTGATGCGAACTTTAGCGCAAAAGCTCGTGCGATTCAAGTGAAGCGGAAAaat                                                                                                                                                                                                   <  1:374331/101‑1 (MQ=255)                                                                                                                                                                                                        cgacgaTCTTCCTCTTGGAAATTGATGCGAACTTTAGCGCAAAAGCTCGTGCGATTCAAGTGAAGCGGAAAAATAATCCATTAATTTTATACGGAATATTGGGCGTTTAAATGTTGGCGATAGCGTTGGTAAACGGATTGATATTGTTCCGCATTTTCCGCTATCGGCGCTGTGCGCGTCGCTTCATTTAACTGTACGAAAGCTCGGCATAATTCCGCCAGCGAACCCTGTTTATTTGCCCACATGGCTTg                  >  1:761639/1‑251 (MQ=255)                                                                                                                                                                                                             aTCTTCCTCTTGGAAATTGATGCGAACTTTAGCGCAAAAGCTCGTGCGATTCAAGTGAAGCGGAAAAATAATCCATTAATTTTATACGGAATATTggg                                                                                                                                                                      >  1:526937/1‑98 (MQ=255)                                                                                                                                                                                                             aTCTTCCTCTTGGAAATTGATGCGAACTTTAGCGCAAAAGCTCGTGCGATTCAAGTGAAGCGGAAAAATAATCCATTAATTTTATACGGAATATTGGGCGTTTAAATGTTGGCGATAGCGTTGGTAAACGGATTGATATTGTTCCGCATTTTCCGCTATCGGCGCTGTGCGCGTCGCTTCATTTAACTGTACGAAAGCTCGGCATAATTCCGCCAGCGAACCCTGTTTATTTGCCCACATGGCTTGAATTg             >  1:835093/1‑251 (MQ=255)                                                                                                                                                                                                               cTTCCTCTTGGAAATTGATGCGAACTTTAGCGCAAAAGCTCGTGCGATTCAAGTGAAGCGGAAAAATAATCCATTAATTTTATACGGAATATTGGGCGTTTAAATGTTGGCGATAGCGTTGGTAAACGGATTGATATTGTTCCGCATTTTCCGCTATCGGCGCTGTGCGCGTCGCTTCATTTAACTGTACGAAAGCTCGGCATAATTCCGCCAGCGAACCCTGTTTATTTGCCCACATGGCTTGAAt               >  1:860579/1‑247 (MQ=255)                                                                                                                                                                                                                 tCCTCTTGGAAATTGATGCGAACTTTAGCGCAAAAGCTCGTGCGATTCAAGTGAAGCGGAAAAATAATCCATTAATTTTATACGGAATATTGGGCGTTTAAATg                                                                                                                                                            <  1:301949/104‑1 (MQ=255)                                                                                                                                                                                                                     ctTGGAAATTGATGCGAACTTTAGCGCAAAAGCTCGTGCGATTCAAGTGAAGCGGAAAAATAATCCATTAATTTTATACGGAATATTGGGCGTTTAAATGTTGGCGATAGCGTTGGTAAACGGATTGATATTGTTc                                                                                                                        <  1:48931/136‑1 (MQ=255)                                                                                                                                                                                                                     ctTGGAAATTGATGCGAACTTTAGCGCAAAAGCTCGTGCGATTCAAGTGAAGCGGAAAAATAATCCATTAATTTTATACGGAATATTGGGCGTTTAAATGTTGGCGATAGCGTTGGTAAACGGATTGATATTGTTCCGCATTTTCCGCTATCGGCGCTGTGCGCGTCGCTTCATTTAACTGTACGaa                                                                     <  1:226943/187‑1 (MQ=255)                                                                                                                                                                                                                     ctTGGAAATTGATGCGAACTTTAGCGCAAAAGCTCGTGCGATTCAAGTGAAGCGGAAAAATAATCCATTAATTTTATACGGAATATTGGGCGTTTAAATGTTGGCGATAGCGTTGGTAAACGGATTGATATTGTTCCGCATTTTCCGCTATCGGCGCTGTGCGCGTCGCTTCATTTAACTGTACGAAAGCTCGGCATAATTCCGCCAGCGAACCCTGTTTATTTg                               <  1:244793/225‑1 (MQ=255)                                                                                                                                                                                                                     ctTGGAAATTGATGCGAACTTTAGCGCAAAAGCTCGTGCGATTCAAGTGAAGCGGAAAAATAATCCATTAATTTTATACGGAATATTGGGCGTTTAAATGTTGGCGATAGCGTTGGTAAACGGATTGATATTGTTCCGCATTTTCCGCTATCGGCGCTGTGCGCGTCGCTTCATTTAACTGTACGAAAGCTCGGCATAATTCCGCCAGCGAACCCTGTTTATTTGCCCACATGGCTTGAATTGCGGCGcc      >  1:630074/1‑250 (MQ=255)                                                                                                                                                                                                                         gAAATTGATGCGAACTTTAGCGCAAAAGCTCGTGCGATTCAAGTGAAGCGGAAAAATAATCCATTAATTTTATACGGAATATTGGGCGTTTAAATGTTGGCGATAGCGTTGGTAAACGGATTGATATTGTTCCGCATTTTCCGCTATCGGCGCTGTGCGCGTCGCTTCATTTAACTGTACGAAAGCTCGGCATAATTCCGCCAGCGAACCCTGTTTATTTGCCCACATGGCTTGAATTGCGGCGCCCAAt  <  1:780181/250‑1 (MQ=255)                                                                                                                                                                                                                                       cTTTAGCGCAAAAGCTCGTGCGATTCAAGTGAAGCGGAAAAATAATCCATTAATTTTATACGGAATATTGGGCGTTTAAATGTTGGCGATAGCGTTGGTAAACGGATTGATATTGTTc                                                                                                                        <  1:312167/118‑1 (MQ=255)                                                                                                                                                                                                                                       cTTTAGCGCAAAAGCTCGTGCGATTCAAGTGAAGCGGAAAAATAATCCATTAATTTTATACGGAATATTGGGCGTTTAAATGTTGGCGATAGCGTTGGTAAACGGATTGATATTGTTCCGCATTTTCCGCTATCGGCGCTGTGCGCGTCGCTTCATTTAACTGTACGAAAg                                                                   <  1:894011/171‑1 (MQ=255)                                                                                                                                                                                                                                       cTTTAGCGCAAAAGCTCGTGCGATTCAAGTGAAGCGGAAAAATAATCCATTAATTTTATACGGAATATTGGGCGTTTAAATGTTGGCGATAGCGTTGGTAAACGGATTGATATTGTTCCGCATTTTCCGCTATCGGCGCTGTGCGCGTCGCTTCATTTAACTGTACGAAAGCTCGGCATAATTCCGCCAGCGAACCCTGttt                                    >  1:634889/1‑202 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                             AACCATGCGATGGCTTCTATCGGGTGACAACGCTGCAACCGAAGCTGATAGTCGGCAATCAATTTCGGTCAAAAGTGCGGTCATTTTATGAAGTGTTTTTTAAAAATAAAATTGAGTGGCGGAAGATCGTAGGAGTTGAACCTACCCGAGACTGCTGGCAGCCTCAACAGGATTTGAAGTCCTGCCGCCTCACCGGAAACGACGATCTTCCTCTTGGAAATTGATGCGAACTTTAGCGCAAAAGCCCGTGCGATTCAAGTGAAGCGGAAAAATAATCCATTAATTTTATACGGAATATTGGGCGTTTAAATGTTGGCGATAGCGTTGGTAAACGGATTGATATTGTTCCGCATTTTCCGCTATCGGCGCTGTGCGCGTCGCTTCATTTAACTGTACGAAAGCTCGGCATAATTCCGCCAGCGAACCCTGTTTATTTGCCCACATGGCTTGAATTGCGGCGCCCAAT  >  1/473956‑474421

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑