Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	400,345	T→G	N259H (AAT→CAT)	unknown ←	COG0859: ADP‑heptose:LPS heptosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	400,345	0	T	G	100.0%	135.6 / NA	39	N259H (AAT→CAT)	unknown	COG0859: ADP‑heptose:LPS heptosyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (26/13);  total (26/13)

ATTTCATCGGGAGAGATTTCATTGACATTTTCAATAAAATTAAGGATATATGTTTTATTTTTACAGTTTGGATTCCAATGCGTAAAGTTTTCTTTATCAGCGAGCTTATAAAATCCAATAATTTTTTTATTTAATCCTGCTGCAATATGAATAATAGACGTATCAGGAGAAATAACGAGGCTAGTATAATGAAGTAATGTTATCGTATCAAAGATATTTTCAGTATTTTCATAGATAAAAGCATTGGTATATTTTTCTGCTATCGTTTTTAACAAAGGGGTTACTTCGGGGTAGGTTAGTAACAGCGCTTTTTTATTTTCGGCTTTAAATTTTTCGATAAACCGATAAATGTTTTGTTCCGTAAATTTTCTGGTGCCGGCTGCACCAAAAAAGTTTATAGCGATGCAATCGACAAGATGATTTTTTTGTATGAAATTTGCAATATCTTCTTTTTTATCACTGTGATTTGGAA  >  1/400119‑400590                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                      aTTTCATCGGGAGAGATTTCATTGACATTTTCAATAAAATTAAGGATATATGTTTTATTTTTACAGTTTGGATTCCAATGCGTAAAGTTTTCTTTATCAGCGAGCTTATAAAATCCAATAATTTTTTTATTTAATCCTGCTGCAATATGAATAATAGACGTATCAGGAGAAATAACGAGGCTAGTATAATGAAGTAATGTTATCGTATCAAAGATATTTTCAGTATGTTCATAGATAAAAGCATTGGtata                                                                                                                                                                                                                               >  1:349406/1‑251 (MQ=255)                                     aaaTTAAGGATATATGTTTTATTTTTACAGTTTGGATTCCAATGCGTAAAGTTTTCTTTATCAGCGAGCTTATAAAATCCAATAATTTTTTTATTTAATCCTGCTGCAATATGAATAATAGACGTATCAGGAGAAATAACGAGGCTAGTATAATGAAGTAATGTTATCGTATCAAAGATATTTTCAGTATGTTCATAGATAAAAGCATTGGTATATTTTTCTGCTATCGTTTTTAACAAAGGGGTTACTTc                                                                                                                                                                                           <  1:437868/251‑1 (MQ=255)                                      aaTTAAGGATATATGTTTTATTTTTACAGTTTGGATTCCAATGCGTAAAGTTTTCTTTATCAGCGAGCTTATAAAATCCAATAATTTTTTTATTTAATCCTGCTGCAATATGAATAATAGACGTATCAGGAGAAATAACGAGGCTAGTATAATGAAGTAATGTTATCGTATCAAAGATATTTTCAGTATGTTCATAGATAAAAGc                                                                                                                                                                                                                                        >  1:274325/1‑205 (MQ=255)                                          aaGGATATATGTTTTATTTTTACAGTTTGGATTCCAATGCGTAAAGTTTTCTTTATCAGCGAGCTTATAAAATCCAATAATTTTTTTATTTAATCCTGCTGCAATATGAATAATAGACGTATCAGGAGAAATAACGAGGCTAGTATAATGAAGTAATGTTATCGTATCAAAGATATTTTCAGTATGTTCATAGATAAAAGCATTGGTATATTTTTCTGCTATCGTTTTTAACAAAGGGGTTACTTCGGGGt                                                                                                                                                                                      <  1:405329/251‑1 (MQ=255)                                                 tatGTTTTATTTTTACAGTTTGGATTCCAATGCGTAAAGTTTTCTTTATCAGCGAGCTTATAAAATCCAATAATTTTTTTATTTAATCCTGCTGCAATATGAATAATAGACGTATCAGGAGAAATAACGAGGCTAGTATAATGAAGTAATGTTATCGTATCAAAGATATTTTCAGTATGTTCATAGATAAAAGCATTGGTATATTTTTCTGCTATCGTTTTTAACAAAGGGGTTACTTCGGGGTAGGTTAg                                                                                                                                                                               <  1:98391/251‑1 (MQ=255)                                                                cAGTTTGGATTCCAATGCGTAAAGTTTTCTTTATCAGCGAGCTTATAAAATCCAATAATTTTTTTATTTAATCCTGCTGCAATATGAATAATAGACGTATCAGGAGAAATAACGAGGCTAGTATAATGAAGTAATGTTATCGTATCAAAGATATTTTCAGTATGTTCATAGATAAAAGc                                                                                                                                                                                                                                        >  1:50423/1‑179 (MQ=255)                                                                  gTTTGGATTCCAATGCGTAAAGTTTTCTTTATCAGCGAGCTTATAAAATCCAATAATTTTTTTATTTAATCCTGCTGCAATATGAATAATAGACGTATCAGGAGAAATAACGAGGCTAGTATAATGAAGTAATGTTATCGTATCAAAGATATTTTCAGTATGTTCATAGATAAAAGc                                                                                                                                                                                                                                        >  1:714445/1‑177 (MQ=255)                                                                                gCGTAAAGTTTTCTTTATCAGCGAGCTTATAAAATCCAATAATTTTTTTATTTAATCCTGCTGCAATATGAATAATAGACGTATCAGGAGAAATAACGAGGCTAGTATAATGAAGTAATGTTATCGTATCAAAGATATTTTCAGTATGTTCATAGATAAAAGc                                                                                                                                                                                                                                        >  1:754929/1‑163 (MQ=255)                                                                                          ttCTTTATCAGCGAGCTTATAAAATCCAATAATTTTTTTATTTAATCCTGCTGCAATATGAATAATAGACGTATCAGGAGAAATAACGAGGCTAGTATAATGAAGTAATGTTATCGTATCAAAGATATTTTCAGTATGTTCATAGATAAAAGCATTGGTATATTTTTCTGCTATCGTTTTTAACAAAGGGGTTACTTCGGGGTAGGTTAGTAACAGCGCTTTTTTATTTTCGGCTTTAAATTTTTCGATaa                                                                                                                                      >  1:62202/1‑251 (MQ=255)                                                                                                            ataAAATCCAATAATTTTTTTATTTAATCCTGCTGCAATATGAATAATAGACGTATCAGGAGAAATAACGAGGCTAGTATAATGAAGTAATGTTATCGTATCAAAGATATTTTCAGTATGTTCATAGATAAAAGc                                                                                                                                                                                                                                        >  1:353374/1‑135 (MQ=255)                                                                                                                                                 atatGAATAATAGACGTATCAGGAGAAATAACGAGGCTAGTATAATGAAGTAATGTTATCGTATCAAAGATATTTTCAGTATGTTCATAGATAAAAGCATTGGTATATTTTTCTGCTATAGTTTTTAACAAAGGGGTTACTTCGGGGt                                                                                                                                                                                      <  1:589443/148‑1 (MQ=255)                                                                                                                                                   atGAATAATAGACGTATCAGGAGAAATAACGAGGCTAGTATAATGAAGTAATGTTATCGTATCAAAGATATTTTCAGTATGTTCATAGATAAAAGc                                                                                                                                                                                                                                        >  1:561432/1‑96 (MQ=255)                                                                                                                                                     gAATAATAGACGTATCAGGAGAAATAACGAGGCTAGTATAATGAAGTAATGTTATCGTATCAAAGATATTTTCAGTATGTTCATAGATAAAAGCATTGGTATATTTTTCTGCTATCGTTTTTAACAAAGGGGTTACTTCGGGGTAGGTTAGTAACAGCGCTTTTTTATTTTCGGCTTTAAATTTTTCGATAAACCGATAAATGTTTTGTTCCGTAAATTTTCTGGTGCCGGCTGCACCAAAAAAGTTtata                                                                           >  1:369488/1‑251 (MQ=255)                                                                                                                                                      aataatAGACGTATCAGGAGAAATAACGAGGCTAGTATAATGAAGTAATGTTATCGTATCAAAGATATTTTCAGTATGTTCATAGATAAAAGCATTGGTATATTTTTCTGCTATCGTTTTTAACAAAGGGGTTACTTCGGGGTAGGTTAGTAACAGCGCTTTTTTATTTTCGGCTTTAAATTTTTCGATAAACCGATAAATGTTTTGTTCCGTAAATTTTCTGGTGCCGGCTGCACCAAAAAAGTTTATAg                                                                          <  1:177315/251‑1 (MQ=255)                                                                                                                                                      aataatAGACGTATCAGGAGAAATAACGAGGCTAGTATAATGAAGTAATGTTATCGTATCAAAGATATTTTCAGTATGTTCATAGATAAAAGCATTGGTATATTTTTCTGCTATCGTTTTTAACAAAGGGGTTACTTCGGGGTAGGTTAGTAACAGCGCTTTTTTATTTTCGGCTTTAAATTTTTCGATAAACCGATAAATGTTTTGTTCCGTAAATTTTCTGGTGCCGGCTGCACCAAAAAAGTTTATAg                                                                          <  1:334497/251‑1 (MQ=255)                                                                                                                                                                       gagaAATAACGAGGCTAGTATAATGAAGTAATGTTATCGTATCAAAGATATTTTCAGTATGTTCATAGATAAAAGCATTGGTATATTTTTCTGCTATCGTTTTTAACAAAGGGGTTACTTCGGGGTAGGTTAGTAACAGCGCTTTTTTATTTTCGGCTTTAAATTTTTCGATAAACCGATAAATGTTTTGTTCCGTAAATTTTCTGGTGCCGGCTGCACTAAAAAAGTTTATAGCGATGCAATCGACAAga                                                         >  1:874651/1‑251 (MQ=255)                                                                                                                                                                              aaCGAGGCTAGTATAATGAAGTAATGTTATCGTATCAAAGATATTTTCAGTATGTTCATAGATAAAAGCATTGGTATATTTTTCTGCTATCGTTTTTAACAAAGGGGTTACTTCGGGGTAGGTTAGTAACAGCGCTTTTTTATTTTCGGCTTTAAATTTTTCGATAAACCGATAAATGTTTTGTTCCGTAAATTTTCTGGTGCCGGCTGCACCAAAAAAGTTTATAGCGATGCAATCGACAAGATGAtttt                                                  >  1:239629/1‑251 (MQ=255)                                                                                                                                                                                   ggCTAGTATAATGAAGTAATGTTATCGTATCAAAGATATTTTCAGTATGTTCATAGATAAAAGCGTTGGTATATTTTTCTGCTATCGTTTTTAACAAAGGGGTTACTTCGGGGTAGGTTAGTAACAGCGCTTTTTTATTTTCGGCTTTAAATTTTTCGATAAACCGATAAATGTTTTGTTCCGTAAATTTTCTGGTGCCGGCTGCACCAAAAAAg                                                                                 >  1:188071/1‑215 (MQ=255)                                                                                                                                                                                   ggCTAGTATAATGAAGTAATGTTATCGTATCAAAGATATTTTCAGTATGTTCATAGATAAAAGCATTGGTATATTTTTCTGCTATCGTTTTTAACAAAGGGGTTACTTCGGGGTAGGtt                                                                                                                                                                                 <  1:826084/119‑1 (MQ=255)                                                                                                                                                                                   ggCTAGTATAATGAAGTAATGTTATCGTATCAAAGATATTTTCAGTATGTTCATAGATAAAAGCATTGGTATATTTTTCTGCTATCGTTTTTAACAAAGGGGTTACTTCGGGGTAGGTTAGTAACAGCGCTTTTTTATTTTCGGCTTTAAATTTTTCGATAAACCGATaaa                                                                                                                             >  1:761241/1‑171 (MQ=255)                                                                                                                                                                                   ggCTAGTATAATGAAGTAATGTTATCGTATCAAAGATATTTTCAGTATGTTCATAGATAAAAGCATTGGTATATTTTTCTGCTATCGTTTTTAACAAAGGGGTTACTTCGGGGTAGGTTAGTAACAGCGCTTTTTTATTTTCGGCTTTAAATTTTTCGATAAACCGATAGATGTTTTGTTCCGTAAATTTTCTGGTGCCGGCTGCACCAAAAAAGTTTATAGCGATGCAATCGACAAGATGATTTTTTTGt                                             >  1:437880/1‑251 (MQ=255)                                                                                                                                                                                   ggCTAGTATAATGAAGTAATGTTATCGTATCAAAGATATTTTCAGTATGTTCATAGATAAAAGCATTGGTATATTTTTCTGCTATCGTTTTTAACAAAGGGGTTACTTCGGGGTAGGTTAGTAACAGCGCTTTTTTATTTTCGGCTTTAAATTTTTCGATAAACCGATAAATGTTTTGTTCCGTAAATTTTCTGGTGCCGGCTGCACCAAAAAAGTTtat                                                                            >  1:552720/1‑220 (MQ=255)                                                                                                                                                                                   ggCTAGTATAATGAAGTAATGTTATCGTATCAAAGATATTTTCAGTATGTTCATAGATAAAAGCATTGGTATATTTTTCTGCTATCGTTTTTAACAAAGGGGTTACTTCGGGGTAGGTTAGTAACAGCGCTTTTTTATTTTCGGCTTTAAATTTTTCGATAAACCGATAAATGTTTTGTTCCGTAAATTTTCTGGTGCCGGCTGCACCAAAAAAGTTTATAGCGATGCAATCGACAAGATGATTTTTTTGt                                             >  1:829364/1‑251 (MQ=255)                                                                                                                                                                                   ggCTAGTATAATGAAGTAATGTTATCGTATCAAAGATATTTTCAGTATGTTCATAGATAAAAGCATTGGTATATTTTTCTGCTATCGTTTTTAACAAAGGGGTTACTTCGGGGTAGGTTAGTAACAGCGCTTTTTTATTTTCGGCTTTAAATTTTTCGATAAACCGATAAATGTTTTGTTCCGTAAATTTTCTGGTGCCGGCTGCACCAAAAAAGTTTATAGCGATGCAATCGACAAGATGATTTTTTTGt                                             >  1:692165/1‑251 (MQ=255)                                                                                                                                                                                        gTATAATGAAGTAATGTTATTGTATCAAAGATATTTTCAGTATGTTCATAGATAAAAGCATTGGTATATTTTTCTGCTATCGTTTTTAACAAAGGGGTTACTTCGGGGTAGGTTAGTAACAGCGCTTTTTTATTTTCGGCTTTAAATTTTTCGATAAACCGATAAATGTTTTGTTCCGTAAATTTTCTGGTGCCGGCTGCACCAAAAAAGTTTATAGCGATGCAATCGACAAGATGATTTTTTTGTATGaa                                        >  1:126128/1‑251 (MQ=255)                                                                                                                                                                                        gTATAATGAAGTAATGTTATCGTATCAAAGATATTTTCAGTATGTTCATAGATAAAAGc                                                                                                                                                                                                                                        <  1:663745/59‑1 (MQ=255)                                                                                                                                                                                        gTATAATGAAGTAATGTTATCGTATCAAAGATATTTTCAGTATGTTCATAGATAAAAGCATTGGTATATTTTTCTGCTATCGTTTTTAACAAAGGGGTTACTTCGGGGTAGGTTAGTAACAGCGCTTTTTTATTTTCGGCTTTAAATTTTTCGATAAACCGATAAATGTTTTGTTCCGTAAATTTTCTGGTGCCGGCTGCACCAAAAAAGTTTATAGCGATGc                                                                    <  1:315627/223‑1 (MQ=255)                                                                                                                                                                                        gTATAATGAAGTAATGTTATCGTATCAAAGATATTTTCAGTATGTTCATAGATAAAAGCATTGGTATATTTTTCTGCTATCGTTTTTAACAAAGGGGTTACTTCGGGGTAGGTTAGTAACAGCGCTTTTTTATTTTCGGCTTTAAATTTTTCGATAAACCGATAAATGTTTTGTTCCGTAAATTTTCTGGTGCCGGCTGCACCAAAAAAGTTTATAGCGATGCAATCGACAAGATGATTTTTTTg                                              >  1:231488/1‑245 (MQ=255)                                                                                                                                                                                        gTATAATGAAGTAATGTTATCGTATCAAAGATATTTTCAGTATGTTCATAGATAAAAGCATTGGTATATTTTTCTGCTATCGTTTTTAACAAAGGGGTTACTTCGGGGTAGGTTAGTAACAGCGCTTTTTTATTTTCGGCTTTAAATTTTTCGATAAACCGATAAATGTTTTGTTCCGTAAATTTTCTGGTGCCGGCTGCACCAAAAAAGTTTATAGCGATGCAATCGACAAGATGATTTTTTTGTATGaa                                        >  1:93013/1‑251 (MQ=255)                                                                                                                                                                                               gAAGTAATGTTATCGTATCAAAGATATTTTCAGTATGTTCATAGATAAAAGCATTGGTATATTTTTCTGCTATCGTTTTTAACAAAGGGGTTACTTCGGGGTAGGTTAGTAACAGCGCTTTTTTATTTTCGGCTTTAAATTTTTCGATAAACCGATa                                                                                                                               >  1:295120/1‑157 (MQ=255)                                                                                                                                                                                                  gTAATGTTATCGTATCAAAGATATTTTCAGTATGTTCATAGATAAAAGCATTGGTATATTTTTCTGCTATCGTTTTTAACAAAGGGGTTACTTCGGGGTAGGTTAGTAACAGCGCTTTTTTATTTTCGGCTTTAAATTTTTCGATAAACCGATAAATGTTTTGTTCCGTAAATTTTCTGGTGCCGGCTGCACCAAAAAAGTTTATAGCGATGCAATCGACAAGATGATTTTTTTGTATGAAATTTGCAata                              >  1:44877/1‑251 (MQ=255)                                                                                                                                                                                                     aTGTTATCGTATCAAAGATATTTTCAGTATGTTCATAGATAAAAGCATTGGTATATTTTTCTGCTATCGTTTTTAACAAAGGGGTTACTTCGGGGTAGGTTAGTAAc                                                                                                                                                                           >  1:471272/1‑107 (MQ=255)                                                                                                                                                                                                                tCAAAGATATTTTCAGTATGTTCATAGATAAAAGCATTGGTATATTTTTCTGCTATCGTTTTTAACAAAGGGGTTACTTCGGGGTAGGTTAGTAACAGCGCTTTTTTATTTTCGGCTTTAAATTTTTCGATAAACCGATAAATGTTTTGTTCCGTAAATTTTCTGGTGCCGGCTGCACCAAAAAAGTTTATAGCGATGCAATCGACAAGATGATTTTTTTGTATGAAATTTGCAATATCTTCTTTTTTATc                <  1:561100/251‑1 (MQ=255)                                                                                                                                                                                                                tCAAAGATATTTTCAGTATGTTCATAGATAAAAGCATTGGTATATTTTTCTGCTATCGTTTTTAACAAAGGGGTTACTTCGGGGTAGGTTAGTAACAGCGCTTTTTTATTTTCGGCTTTAAATTTTTCGATAAACCGATAAATGTTTTGTTCCGTAAATTTTCTGGTGCCGGCTGCACCAAAAAAGTTTATAGCGATGCAATCGACAAGATGATTTTTTTGTATGAAATTTGCAATATCTTCTTTTTTATc                <  1:858419/251‑1 (MQ=255)                                                                                                                                                                                                                 cAAAGATATTTTCAGTATGTTCATAGATAAAAGCATTGGTATATTTTTCTGCTATCGTTTTTAACAAAGGGGTTACTTCGGGGTAGGTTAGTaa                                                                                                                                                                            >  1:232410/1‑94 (MQ=255)                                                                                                                                                                                                                         ttttCAGTATGTTCATAGATAAAAGCATTGGTATATTTTTCTGCTATCGTTTTTAACAAAGGGGTTACTTCGGGGTAGGTTAGTAACAGCGCTTTTTTATTTTCGGCTTTAAATTTTTCGATAAACCGATAAATGTTTTGTTCCGTAAATTTTCTGGTGCCGGCTGCACCAAAAAAGTTTATAGCGATGCAATCGACAAGATGATTTTTTTGTATGAAATTTGCAATATCTTCTTTTTTATc                >  1:2916/1‑242 (MQ=255)                                                                                                                                                                                                                            tCAGTATGTTCATAGATAAAAGCATTGGTATATTTTTCTGCTATCGTTTTTAACAAAGGGGTTACTTCGGGGTAGGTTAGTAACAGCGCTTTTTTATTTTCGGCTTTAAATTTTTCGATAAACCGATAAATGTTTTGTTCCGTAAATTTTCTGGTGCCGGCTGCACCAAAAAAGTTTATAGCGATGCAATc                                                                <  1:816949/191‑1 (MQ=255)                                                                                                                                                                                                                               gTATGTTCATAGATAAAAGCATTGGTATATTTTTCTGCTATCGTTTTTAACAAAGGGGTTACTTCGGGGTAGGTTAGTAACAGCGCTTTTTTATTTTCGGCTTTAAATTTTTCGATAAACCGATAAATGTTTTGTTCCGTAAATTTTCTGGTGCCGGCTGCACCAAAAAAGTTTATAGCGATGCAATCGACAAGATGATTTTTTTGTATGAAATTTGCAATATCTTCTTTTTTATCACTGTGATTTGGaa  >  1:255911/1‑250 (MQ=255)                                                                                                                                                                                                                               gTATGTTCATAGATAAAAGCATTGGTATATTTTTATGCTATCGTTTTTAACAAAGGGGTTACTTCGGGGTAGGTTAGTAACAGCGCTTTTTTATTTTCGGCTTTAAATTTTTCGATAAACCGATAAATGTTTTGTTCCGTAAATTTTCTGGTGCCGGCTGCACCAAAAAAGTTTATAGCGATGCAATCGACAAGATGATTTTTTTGTATGAAATTTGCAATATCTTCTTTTTTATc                <  1:253949/236‑1 (MQ=255)                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                      ATTTCATCGGGAGAGATTTCATTGACATTTTCAATAAAATTAAGGATATATGTTTTATTTTTACAGTTTGGATTCCAATGCGTAAAGTTTTCTTTATCAGCGAGCTTATAAAATCCAATAATTTTTTTATTTAATCCTGCTGCAATATGAATAATAGACGTATCAGGAGAAATAACGAGGCTAGTATAATGAAGTAATGTTATCGTATCAAAGATATTTTCAGTATTTTCATAGATAAAAGCATTGGTATATTTTTCTGCTATCGTTTTTAACAAAGGGGTTACTTCGGGGTAGGTTAGTAACAGCGCTTTTTTATTTTCGGCTTTAAATTTTTCGATAAACCGATAAATGTTTTGTTCCGTAAATTTTCTGGTGCCGGCTGCACCAAAAAAGTTTATAGCGATGCAATCGACAAGATGATTTTTTTGTATGAAATTTGCAATATCTTCTTTTTTATCACTGTGATTTGGAA  >  1/400119‑400590

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑