Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	350,636	A→G	L144P (CTG→CCG)	unknown ←	putative TonB‑dependent receptor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	350,636	0	A	G	100.0%	185.7 / NA	56	L144P (CTG→CCG)	unknown	putative TonB‑dependent receptor
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (31/25);  total (31/25)

ACCGCACTTAACGCCGCATTATCCAAATCTTCCGCGCCTGAACTTTTTACGATGCTCGCCCCACTAATGGAACCATCTGCACCGATGGAGAAAGAAACGGTCACAATGCCTTGCTTACGCATCATTTTGGCACGTGTTGGGTAGCGTTTATGTTTCTCAATTTCACGTCGAATTGCCGATAAATAGGCATTTCTTTCATCGCTGCTGGCGCCACTGCCTGCCAAATTCGGATTATTGGTTGTCACCAGACCGGTTGATGTGGCTTTAGAATTCACCGAAGCATTGGAATCTACCGTTTTGTCACCCACAGGCACATCAGTTCTCGGTTTTTGCTTCGGCTTCTCTTTCGGTTTTTCCTTTGGCTTTTCTTTCGGCTTCTCTTTTGGTTTTTCCGGTTCTTTCGGTTTTTCAACTTTCGGCGGTTCCGGTTTTACCGTCGGATCATCCACCGCTTCCTGCTTTTCAGGTTCCGGCGCTTTTTCTTCTACCGGTG  >  1/350390‑350882                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                       aCCGCACTTAACGCCGCATTATCCAAATCTTCCGCGCCTGAACTTTTTACGATGCTCGCCCCACTAATGGAACCATCTGCACCGATGGAGAAAGAAACGGTCACAATGCCTTGCTTACGCATCATTTTGGCACGTGTTGGGTAGCGTTTATGTTTCTCAATTTCACGTCGAATTGCCGATAAATAGGCATTTCTTTCATCGCTGCTGGCGCCACTGCCTGCCAAATTCGGATTATTGGTTGTCACCGGAc                                                                                                                                                                                                                                                     <  1:119071/250‑1 (MQ=255) aCCGCACTTAACGCCGCATTATCCAAATCTTCCGCGCCTGAACTTTTTACGATGCTCGCCCCACTAATGGAACCATCTGCACCGATGGAGAAAGAAACGGTCACAATGCCTTGCTTACGCATCATTTTGGCACGTGTTGGGTAGCGTTTATGTTTCTCAATTTCACGTCGAATTGCCGATAAATAGGCATTTCTTTCATCGCTGCTGGCGCCACTGCCTGCCAAATTCGGATTATTGGTTGTCACCGGAc                                                                                                                                                                                                                                                     <  1:215584/250‑1 (MQ=255)     cACTTAACGCCGCATTATCCAAATCTTCCGCGCCTGAACTTTTTACGATGCTCGCCCCACTAATGGAACCATCTGCACCGATGGAGAAAGAAACGGTCACAATGCCTTGCTTACGCATCATTTTGGCACGTGTTGGGTAGCGTTTATGTTTCTCAATTTCACGTCGAATTGCCGATAAATAGGCATTTCTTTCATCGCTGCTGGCGCCACTGCCTGCCAAATTCGGATTATTGGTTGTCACCGGACCGGtt                                                                                                                                                                                                                                                >  1:66346/1‑251 (MQ=255)       cTTAACGCCGCATTATCCAAATCTTCCGCGCCTGAACTTTTTACGATGCTCGCCCCACTAATGGAACCATCTGCACCGATGGAGAAAGAAACGGTCACAATGCCTTGCTTACGCATCATTTTGGCACGTGTTGGGTAGCGTTTATGTTTCTCAATTTCACGTCGAATTGCCGATAAATAGGCATTTCTTTCATCGCTGCTGGCGCCACTGCCTGCCAAATTCGGATTATTGGTTGTCACCGGACCGGTTGa                                                                                                                                                                                                                                              >  1:138973/1‑251 (MQ=255)       cTTAACGCCGCATTATCCAAATCTTCCGCGCCTGAACTTTTTACGATGCTCGCCCCACTAATGGAACCATCTGCACCGATGGAGAAAGAAACGGTCACAATGCCTTGCTTACGCATCATTTTGGCACGTGTTGGGTAGCGTTTATGTTTCTCAATTTCACGTCGAATTGCCGATAAATAGGCATTTCTTTCATCGCTGCTGGCGCCACTGCCTGCCAAATTCGGATTATTGGTTGTCACCGGACCGGTTGa                                                                                                                                                                                                                                              >  1:431702/1‑251 (MQ=255)       cTTAACGCCGCATTATCCAAATCTTCCGCGCCTGAACTTTTTACGATGCTCGCCCCACTAATGGAACCATCTGCACCGATGGAGAAAGAAACGGTCACAATGCCTTGCTTACGCATCATTTTGGCACGTGTTGGGTAGCGTTTATGTTTCTCAATTTCACGTCGAATTGCCGATAAATAGGCATTTCTTTCATCGCTGCTGGCGCCACTGCCTGCCAAATTCGGATTATTGGTTGTCACCGGACCGGTTGa                                                                                                                                                                                                                                              >  1:628788/1‑251 (MQ=255)       cTTAACGCCGCATTATCCAAATCTTCCGCGCCTGAACTTTTTACGATGCTCGCCCCACTAATGGAACCATCTGCACCGATGGAGAAAGAAACGGTCACAATGCCTTGCTTACGCATCATTTTGGCACGTGTTGGGTAGCGTTTATGTTTCTCAATTTCACGTCGAATTGCCGATAAATAGGCATTTCTTTCATCGCTGCTGGCGCCACTGCCTGCCAAATTCGGATTATTGGTTGTCACCGGACCGGTTGa                                                                                                                                                                                                                                              >  1:252182/1‑251 (MQ=255)       cTTAACGCCGCATTATCCAAATCTTCCGCGCCTGAACTTTTTACGATGCTCGCCCCACTAATGGAACCATCTGCACCGATGGAGAAAGAAACGGTCACAATGCCTTGCTTACGCATCATTTTGGCACGTGTTGGGTAGCGTTTATGTTTCTCAATTTCACGTCGAATTGCCGATAAATAGGCATTTCTTTCATCGCTGCTGGCGCCACTGCCTGCCAAATTCGGATTATTGGTTGTCACCGGACCGGTTGa                                                                                                                                                                                                                                              >  1:246760/1‑251 (MQ=255)         tAACGCCGCATTATCCAAATCTTCCGCGCCTGAACTTTTTACGATGCTCGCCCCACTAATGGAACCATCTGCACCGATGGAGAAAGAAACGGTCACAATGCCTTGCTTACGCATCATTTTGGCACGTGTTGGGTAGCGTTTATGTTTCTCAATTTCACGTCGAATTGCCGATAAATAGGCATTTCTTTCATCGCTGCTGGCGCCACTGCCTGCCAAATTCGGATTATTGGTTGTCACCGGACCGGTTGAtg                                                                                                                                                                                                                                            <  1:33983/251‑1 (MQ=255)                     aTCCAAATCTTCCGCGCCTGAACTTTTTACGATGCTCGCCCCACTAATGGAACCATCTGCACCGATGGAGAAAGAAACGGTCACAATGCCTTGCTTACGCATCATTTTGGCACGTGTTGGGTAGCGTTTATGTTTCTCAATTTCACGTCGAATTGCCGATAAATAGGCATTTCTTTCATCGCTGCTGGCGCCACTGCCTGCCAAATTCGGATTATTGGTTGTCACCGGACCGGTTGAtgt                                                                                                                                                                                                                                           >  1:442121/1‑240 (MQ=255)                                          aCTTTTTACGATGCTCGCCCCACTAATGGAACCATCTGCACCGATGGAGAAAGAAACGGTCACAATGCCTTGCTTACGCATCATTTTGGCACGTGTTGGGTAGCGTTTATGTTTCTCAATTTCACGTCGAATTGCCGATAAATAGGCATTTCTTTCATCGCTGCTGGCGCCACTGCCTGCCAAATTCGGATTATTGGTTGTCACCGGACCGGTTGATGTGGCTTTAGAATTCACCGAAGCATTGGAATCTa                                                                                                                                                                                                           >  1:826292/1‑251 (MQ=255)                                                              cACTAATGGAACCATCTGCACCGATGGAGAAAGAAACGGTCACAATGCCTTGCTTACGCATCATTTTGGCACGTGTTGGGTAGCGTTTATGTTTCTCAATTTCACGTCGAATTGCCGATAAATAGGCATTTCTTTCATCGCTGCTGGCGCCACTGCCTGCCAAATTCGGATTATTGGTTGTCACCGGACCGGTTGATGTGGCTTTAGAATTCACCGAAGCATTGGAATCTACCGTTTTGTCACCCACAGGc                                                                                                                                                                                       <  1:90571/251‑1 (MQ=255)                                                                cTAATGGAACCATCTGCACCGATGGAGAAAGAAACGGTCACAATGCCTTGCTTACGCATCATTTTGGCACGTGTTGGGTAGCGTTTATGTTTCTCAATTTCACGTCGAATTGCCGATAAATAGGCATTTCTTTCATCGCTGCTGGCGCCACTGCCTGCCAAATTCGGATTATTGGTTGTCACCGGACCGGTTGATGTGGCTTTAGAATTCACCGAAGCATTGGAATCTACCGTTTTGTCAcc                                                                                                                                                                                              <  1:553499/242‑1 (MQ=255)                                                                cTAATGGAACCATCTGCACCGATGGAGAAAGAAACGGTCACAATGCCTTGCTTACGCATCATTTTGGCACGTGTTGGGTAGCGTTTATGTTTCTCAATTTCACGTCGAATTGCCGATAAATAGGCATTTCTTTCATCGCTGCTGGCGCCACTGCCTGCCAAATTCGGATTATTGGTTGTCACCGGACCGGTTGATGTGGCTTTAGAATTCACCGAAGCATTGGAATCTACCGTTTTGTCACCCACAGGca                                                                                                                                                                                      >  1:558996/1‑250 (MQ=255)                                                                 tAATGGAACCATCTGCACCGATGGAGAAAGAAACGGTCACAATGCCTTGCTTACGCATCATTTTGGCACGTGTTGGGTAGCGTTTATGTTTCTCAATTTCACGTCGAATTGCCGATAAATAGGCATTTCTTTCATCGCTGCTGGCGCCACTGCCTGCCAAATTCGGATTATTGGTTGTCACCGGACCGGTTGATGTGGCTTTAGAATTCACCGAAGCATTGGAATCTACCGTTTTGTCACCCACAGGcaca                                                                                                                                                                                    >  1:336357/1‑251 (MQ=255)                                                                          cATCTGCACCGATGGAGAAAGAAACGGTCACAATGCCTTGCTTACGCATCATTTTGGCACGTGTTGGGTAGCGTTTATGTTTCTCAATTTCACGTCGAATTGCCGATAAATAGGCATTTCTTTCATCGCTGCTGGCGCCACTGCCTGCCAAATTCGGATTATTGGTTGTCACCGGACCGGTTGAtgt                                                                                                                                                                                                                                           <  1:396653/187‑1 (MQ=255)                                                                          cATCTGCACCGATGGAGAAAGAAACGGTCACAATGCCTTGCTTACGCATCATTTTGGCACGTGTTGGGTAGCGTTTATGTTTCTCAATTTCACGTCGAATTGCCGATAAATAGGCATTTCTTTCATCGCTGCTGGCGCCACTGCCTGCCAAATTCGGATTATTGGTTGTCACCGGACCGGTTGATGTGGCTTTAGAATTCACCGAAGCATTGGAATCTACCGTTTTGTCACCCACAGGCACATCAGTtct                                                                                                                                                                            >  1:74497/1‑250 (MQ=255)                                                                             cTGCACCGATGGAGAAAGAAACGGTCACAATGCCTTGCTTACGCATCATTTTGGCACGTGTTGGGTAGCGTTTATGTTTCTCAATTTCACGTCGAATTGCCGATAAATAGGCATTTCTTTCATCGCTGCTGGCGCCACTGCCTGCCAAATTCGGATTATTGGTTGTCACCGGACCGGTTGATGTGGCTTTAGAATTCACCGAAGCATTGGAATCTACCGTTTTGTCACCCACAGGCACATCAGTTCTCGGt                                                                                                                                                                        <  1:193689/251‑1 (MQ=255)                                                                                  ccGATGGAGAAAGAAACGGTCACAATGCCTTGCTTACGCATCATTTTGGCACGTGTTGGGTAGCGTTTATGTTTCTCAATTTCACGTCGAATTGCCGATAAATAGGCATTTCTTTCATCGCTGCTGGCGCCACTGCCTGCCAAATTCGGATTATTGGTTGTCACCGGACCGGTTGATGTGGCTTTAGAATTCACCGAAGCATTGGAATCTACCGTTTTGTCACCCACAGGCACATCAGTTCTCGGTTTTTg                                                                                                                                                                   <  1:243705/251‑1 (MQ=255)                                                                                   cGATGGAGAAAGAAACGGTCACAATGCCTTGCTTACGCATCATTTTGGCACGTGTTGGGTAGCGTTTATGTTTCTCAATTTCACGTCGAATTGCCGATAAATAGGCATTTCTTTCATCGCTGCTGGCGCCACTGCCTGCCAAATTCGGATTATTGGTTGTCACCGGACCGGTTGATGTGGCTTTAGAATTCACCGAAGCATTGGAATCTACCGTTTTGTCACCCACAGGCACATCAGTTCTCGGTTTTTGc                                                                                                                                                                  <  1:140411/251‑1 (MQ=255)                                                                                    gATGGAGAAAGAAACGGTCACAATGCCTTGCTTACGCATCATTTTGGCACGTGTTGGGTAGCGTTTATGTTTCTCAATTTCACGTCGAATTGCCGATAAATAGGCATTTCTTTCATCGCTGCTGGCGCCACTGCCTGCCAAATTCGGATTATTGGTTGTCACCGGACCGGTTGATGTGGCTTTAGAATTCACCGAAGCATTGGAATCTACCGTTTTGTCACCCACAGGCACATCAGTTCTCGGTTTTTg                                                                                                                                                                   >  1:695647/1‑249 (MQ=255)                                                                                          gaaagaaaCGGTCACAATGCCTTGCTTACGCATCATTTTGGCACGTGTTGGGTAGCGTTTATGTTTCTCAATTTCACGTCGAATTGCCGATAAATAGGCATTTCTTTCATCGCTGCTGGCGCCACTGCCTGCCAAATTCGGATTATTGGTTGTCACCGGACCGGTTGATGTGGCTTTAGAATTCACCGAAGCATTGGAATCTACCGTTTTGTCACCCACAGGCACATCAGTTCTCGGTTTTTGCTTCgg                                                                                                                                                             >  1:287219/1‑249 (MQ=255)                                                                                               aaaCGGTCACAATGCCTTGCTTACGCATCATTTTGGCACGTGTTGGGTAGCGTTTATGTTTCTCAATTTCACGTCGAATTGCCGATAAATAGGCATTTCTTTCATCGCTGCTGGCGCCACTGCCTGCCAAATTCGGATTATTGGTTGTCACCGGACCGGTTGATGTGGCTTTAGAATTCACCGAAGCATTGGAATCTACCGTTTTGTCACCCACAGGCACATCAGTTCTCGGTTTTTGCTTCGGCTtctc                                                                                                                                                       <  1:724731/250‑1 (MQ=255)                                                                                                   ggTCACAATGCCTTGCTTACGCATCATTTTGGCACGTGTTGGGTAGCGTTTATGTTTCTCAATTTCACGTCGAATTGCCGATAAATAGGCATTTCTTTCATCGCTGCTGGCGCCACTGCCTGCCAAATTCGGATTATTGGTTGTCACCGGACCGGTTGATGTGGCTTTAGAATTCACCGAAGCATTGGAATCTACCGTTTTGTCACCCACAGGCACATCAGTTCTCGGTTTTTGCTTCGGCTTCTCTTTCg                                                                                                                                                  >  1:795774/1‑251 (MQ=255)                                                                                                    gTCACAATGCCTTGCTTACGCATCATTTTGGCACGTGTTGGGTAGCGTTTATGTTTCTCAATTTCACGTCGAATTGCCGATAAATAGGCATTTCTTTCATCGCTGCTGGCGCCACTGCCTGCCAAATTCGGATTATTGGTTGTCACCGGACCGGTTGAtg                                                                                                                                                                                                                                            <  1:876288/160‑1 (MQ=255)                                                                                                        caATGCCTTGCTTACGCATCATTTTGGCACGTGTTGGGTAGCGTTTATGTTTCTCAATTTCACGTCGAATTGCCGATAAATAGGCATTTCTTTCATCGCTGCTGGCGCCACTGCCTGCCAAATTCGGATTATTGGTTGTCACCGGACCGGTTGATGTGGCTTTAGAATTCACCGAAGCATTGGAATCTACCGTTTTGTCACCCACAGGCACATCAGTTCTCGGTTTTTGCTTCGGCTTCTCTTTCGGtttt                                                                                                                                             >  1:881901/1‑251 (MQ=255)                                                                                                            gCCTTGCTTACGCATCATTTTGGCACGTGTTGGGTAGCGTTTATGTTTCTCAATTTCACGTCGAATTGCCGATAAATAGGCATTTCTTTCATCGCTGCTGGCGCCACTGCCTGCCAAATTCGGATTATTGGTTGTCACCGGACCGGTTGATGTGGCTTTAGaa                                                                                                                                                                                                                                 <  1:103343/163‑1 (MQ=255)                                                                                                                tGCTTACGCATCATTTTGGCACGTGTTGGGTAGCGTTTATGTTTCTCAATTTCACGTCGAATTGCCGATAAATAGGCATTTCTTTCATCGCTGCTGGCGCCACTGCCTGCCAAATTCGGATTATTGGTTGTCACCGGACCGGTTGATGTGGCTTTAGAATTCACCGAAGCATTGGAATCTACCGTTTTGTCACCCACAGGcac                                                                                                                                                                                     <  1:94218/203‑1 (MQ=255)                                                                                                                tGCTTACGCATCATTTTGGCACGTGTTGGGTAGCGTTTATGTTTCTCAATTTCACGTCGAATTGCCGATAAATAGGCATTTCTTTCATCGCTGCTGGCGCCACTGCCTGCCAAATTCGGATTATTGGTTGTCACCGGACCGGTTGATGTGGCTTTAGAATTCACCGAAGCATTGGAATCTACCGTTTTGTCACCCACAGGCACATCAGTTCTCGGTTTTTGCTTCGGCTTCTCTTTCGGTTTTTCCTTTgg                                                                                                                                     <  1:176571/251‑1 (MQ=255)                                                                                                                      cGCATCATTTTGGCACGTGTTGGGTAGCGTTTATGTTTCTCAATTTCACGTCGAATTGCCGATAAATAGGCATTTCTTTCATCGCTGCTGGCGCCACTGCCTGCCAAATTCGGATTATTGGTTGTCACCGGACCGGTTGAtgtg                                                                                                                                                                                                                                          <  1:420234/144‑1 (MQ=255)                                                                                                                       gCATCATTTTGGCACGTGTTGGGTAGCGTTTATGTTTCTCAATTTCACGTCGAATTGCCGATAAATAGGCATTTCTTTCATCGCTGCTGGCGCCACTGCCTGCCAAATTCGGATTATTGGTTGTCACCGGACCGGTTGATGTGGCTTTAGAATTCACCGAAGCATTGGAATCTACCGTTTTGTCACCCACAGGCACATCAGTTCTCGGTTTTTGCTTCGGCTTCTCTTTCGGTTTTTcc                                                                                                                                          >  1:183333/1‑239 (MQ=255)                                                                                                                          tcatTTTGGCACGTGTTGGGTAGCGTTTATGTTTCTCAATTTCACGTCGAATTGCCGATAAATAGGCATTTCTTTCATCGCTGCTGGCGCCACTGCCTGCCAAATTCGGATTATTGGTTGTCACCGGACCGGTTGATGTGGCTTTAGAATTCACCGAAGCATTGGAATCTACCGTTTTGTCACCCACAGGCACATCAGTTCTCGGTTTTTGCTTCGGCTTCTCTTTCGGTTTTTCCt                                                                                                                                         >  1:873073/1‑237 (MQ=255)                                                                                                                             ttttGGCACGTGTTGGGTAGCGTTTATGTTTCTCAATTTCACGTCGAATTGCCGATAAATAGGCATTTCTTTCATCGCTGCTGGCGCCACTGCCTGCCAAATTCGGATTATTGGTTGTCACCGGACCGGTTGATGTGGCTTTAGAATTCACCGAAGCATTGGAATCTACCGTTTTGTCACCCACAGGCACATCAGTTCTCGGTTTTTGCTTCGGCTTCTCTTTCGGTTTTTCCTTTGGCTTTTCTTTCgg                                                                                                                         >  1:585047/1‑250 (MQ=255)                                                                                                                                      gtgtTGGGTAGCGTTTATGTTTCTCAATTTCACGTCGAATTGCCGATAAATAGGCATTTCTTTCATCGCTGCTGGCGCCACTGCCTGCCAAATTCGGATTATTGGTTGTCACCGGACCGGTTGATGTGGCTTTAGAATTCACCGAAGCATTGGaa                                                                                                                                                                                                               >  1:413368/1‑155 (MQ=255)                                                                                                                                        gtTGGGTAGCGTTTATGTTTCTCAATTTCACGTCGAATTGCCGATAAATAGGCATTTCTTTCATCGCTGCTGGCGCCACTGCCTGCCAAATTCGGATTATTGGTTGTCACCGGACCGGTTGATGTGGCTTTAGAATTCACCGAAGCATTGGAATCTACCGTTTTGTCACCCACAGGCACATCAGTTCTCGGTTTTTGCTTCGGCTTCTCTTTCGGTTTTTCCTTTGGCTTTTCTTTCGGCTTCTCTTTTgg                                                                                                             >  1:29144/1‑251 (MQ=255)                                                                                                                                          tGGGTAGCGTTTATGTTTCTCAATTTCACGTCGAATTGCCGATAAATAGGCATTTCTTTCATCGCTGCTGGCGCCACTGCCTGCCAAATTCGGATTATTGGTTGTCACCGGACCGGTTGATGTGGCTTTAGAATTCACCGAAGCATTGGAATCTACCGTTTTGTCACCCACAGGCACATCAGTTCTCGGTTTTTGCTTCGGCTTCTCTTTCGGTTTTTCCTTTGGCTTTTCTTTCGGCTTCTCTTTTGGtt                                                                                                           <  1:527180/251‑1 (MQ=255)                                                                                                                                           gggTAGCGTTTATGTTTCTCAATTTCACGTCGAATTGCCGATAAATAGGCATTTCTTTCATCGCTGCTGGCGCCACTGCCTGCCAAATTCGGATTATTGGTTGTCACCGGACCGGTTGATGTGGCTTTAGAATTCACCGAAGCATTGGAATCTACCGTTTTGTCACCCACAGGCACATCAGTTCTCGGTTTTTGCTTCGGCTTCTCTTTCGGTTTTTCCTTTGGCTTTTCTTTCGGCTTCTCTTTTGGttt                                                                                                          >  1:422735/1‑251 (MQ=255)                                                                                                                                                gCGTTTATGTTTCTCAATTTCACGTCGAATTGCCGATAAATAGGCATTTCTTTCATCGCTGCTGGCGCCACTGCCTGCCAAATTCGGATTATTGGTTGTCACCGGACCGGTTGATGTGGCTTTAGAATTCACCGAAGCATTGGAATCTACCGTTTTGTCACCCACAGGCACATCAGTTCTCGGTTTTTGCTTCGGCTTCTCTTTCGGTTTTTCCTTTGGCTTTTCTTTCGGCTTCTCTTTTGGTTTTTCCg                                                                                                     >  1:596525/1‑251 (MQ=255)                                                                                                                                                    ttATGTTTCTCAATTTCACGTCGAATTGCCGATAAATAGGCATTTCTTTCATCGCTGCTGGCGCCACTGCCTGCCAAATTCGGATTATTGGTTGTCACCGGACCGGTTGATGTGGCTTTAGAATTCACCGAAGCATTGGAATCTACCGTTTTGTCACCCACAGGCACATCAGTTCTCGGTTTTTGCTTCGGCTTCTCTTTCGGTTTTTCCTTTGGCTTTTCTTTCGGCTTCTCTTTTGGTTTTTCCGGTTc                                                                                                 <  1:754415/251‑1 (MQ=255)                                                                                                                                                    ttATGTTTCTCAATTTCACGTCGAATTGCCGATAAATAGGCATTTCTTTCATCGCTGCTGGCGCCACGGCCTGCCAAATTCGGATTATTGGTTGTCACCGGACCGGTTGATGTGGCTTTAGAATTCACCGAAGCATTGGAATCTACCGTTTTGTCACCCACAGGCACATCAGTTCTCGGTTTTTGCTTCGGCTTCTCTTTCGGTTTTTCCTTTGGCTTTTCTTTCGGCTTCTCTTTTGGTTTTTCCGGTTc                                                                                                 <  1:339206/251‑1 (MQ=255)                                                                                                                                                         tttCTCAATTTCACGTCGAATTGCCGATAAATAGGCATTTCTTTCATCGCTGCTGGCGCCACTGCCTGCCAAATTCGGATTATTGGTTGTCACCGGACCGGTTGATGTGGCTTTAGAATTCACCGAAGCATTGGAATCTACCGTTTTGTCACCCACAGGCACATCAg                                                                                                                                                                                <  1:612701/167‑1 (MQ=255)                                                                                                                                                                aTTTCACGTCGAATTGCCGATAAATAGGCATTTCTTTCATCGCTGCTGGCGCCACTGCCTGCCAAATTCGGATTATTGGTTGTCACCGGACCGGTTGATGTGGCTTTAGAATTCACCGAAGCATTGGAATCTACCGTTTTGTCACCCACAGGCACATCAGTTCTCGGTTTTTGCTTCGGCTTCTCTTTCGGTTTTTCCTTTGGCTTTTCTTTCGGCTTCTCTTTTGGTTTTTCCGGTTCTTTCGGttttt                                                                                      >  1:519197/1‑250 (MQ=255)                                                                                                                                                                   tCACGTCGAATTGCCGATAAATAGGCATTTCTTTCATCGCTGCTGGCGCCACTGCCTGCCAAATTCGGATTATTGGTTGTCACCGGACCGGTTGATGTGGCTTTAGAATTCACCGAAGCATTGGAATCTACCGTTTTGTCACCCACAGGCACATCAGTTCTCGGTTTTTGCTTCGGCTTCTCTTTCGGTTTTTCCTTTGGCTTTTCTTTCGGCTTCTCTTTTGGTTTTTCCGGTTCTTTCGGTTTTTCAAc                                                                                  <  1:506196/251‑1 (MQ=255)                                                                                                                                                                        tCGAATTGCCGATAAATAGGCATTTCTTTCATCGCTGCTGGCGCCACTGCCTGCCAAATTCGGATTATTGGTTGTCACCGGACCGGTTGATGTGGCTTTAGAATTCACCGAAGCATTGGAATCTACCGTTTTGTCACCCACAGGCACATCAGTTCTCGGTTTTTGCTTCGGCTTCTCTTTCGGTTTTTCCTTTGGCTTTTCTTTCGGCTTCTCTTTTGGTTTTTCCGGTTCTTTCGGTTTTTCAACTTTcg                                                                             >  1:15324/1‑251 (MQ=255)                                                                                                                                                                               gCCGATAAATAGGCATTTCTTTCATCGCTGCTGGCGCCACTGCCTGCCAAATTCGGATTATTGGTTGTCACCGGACCGGTTGATGTGGCTTTAGAATTCACCGAAGCATTGGAATCTACCGTTTTGTCACCCACAGGCACATCAGTTCTCGGTTTTTGCTTCGGCTTCTCTTTCGGTTTTTCCTTTGGCTTTTCTTTCGGCTTCTCTTTTGGTTTTTCCGGTTCTTTCGGTTTTTCAACTTTCGGCGGtt                                                                       >  1:349017/1‑250 (MQ=255)                                                                                                                                                                               gCCGATAAATAGGCATTTCTTTCATCGCTGCTGGCGCCACTGCCTGCCAAATTCGGATTATTGGTTGTCACCGGACCGGTTGATGTGGCTTTAGAATTCACCGAAGCATTGGAATCTACCGTTTTGTCACCCACAGGCACATCAGTTCTCGGTTTTTGCTTCGGCTTCTCTTTCGGTTTTTCCTTTGGCTTTTCTTTCGGCTTCTCTTTTGGTTTTTCCGGTTCTTTCGGTTTTTCAACTTTCGGCGGtt                                                                       >  1:400499/1‑250 (MQ=255)                                                                                                                                                                                ccGATAAATAGGCATTTCTTTCATCGCTGCTTGCGCCACTGCCTGCCAAATTCGGATTATTGGTTGTCACCGGACCGGTTGATGTGGCTTTAGAATTCACCGAAGCATTGGAATCTACCGTTTTGTCACCCACAGGCACATCAGTTCTCGGTTTTTGCTTCGGCTTCTCTTTCGGTTTTTCCTTTGGCTTTTCTTTCGGCTTCTCTTTTGGTTTTTCCGGTTCTTTCGGTTTTTCAACTTTCGGCGGTTc                                                                      >  1:208674/1‑250 (MQ=255)                                                                                                                                                                                   aTAAATAGGCATTTCTTTCATCGCTGCTGGCGCCACTGCCTGCCAAATTCGGATTATTGGTTGTCACCGGACCGGTTGATGTGGCTTTAGa                                                                                                                                                                                                                                  >  1:56480/1‑91 (MQ=255)                                                                                                                                                                                           tcATTTCTTTCATCGCTGCTGGCGCCACTGCCTGCCAAATTCGGATTATTGGTTGTCACCGGACCGGTTGATGTGGCTTTAGAATTCACCGAAGCATTGGAATCTACCGTTTTGTCACCCACAGGCACATCAGTTCTCGGTTTTTGCTTCGGCTTCTCTTTCGGTTTTTCCTTTGGCTTTTCTTTCGGCTTCTCTTTTGGTTTTTCCGGTTCTTTCGGTTTTTCAACTTTCGGCGGTTCCGGTTTTAcc                                                            <  1:180727/248‑1 (MQ=255)                                                                                                                                                                                                     cATCGCTGCTGGCGCCACTGCCTGCCAAATTCGGATTATTGGTTGTCACCGGACCGGTTGATGTGGCTTTAGAATTCACCGAAGCATTGGAATCTACCGTTTTGTCACCCACAGGCACATCAGTTCTCGGTTTTTGCTTCGGCTTCTCTTTCGGTTTTTCCTTTGGCTTTTCTTTCGGCTTCTCTTTTGGTTTTTCCGGTTCTTTCGGTTTTTCAACTTTCGGCGGTTCCGGTTTTACCGTCGGATCATcc                                                <  1:8515/251‑1 (MQ=255)                                                                                                                                                                                                       tCGCTGCTGGCGCCACTGCCTGCCAAATTCGGATTATTGGTTGTCACCGGACCGGTTGATGTGGCTTTAGAATTCACCGAAGCATTGGAATCTACCGTTTTGTCACCCACAGGCACATCAGTTCTCGGTTTTTGCTTCGGCTTCTCTTTCGGTTTTTCCTTTGGCTTTTCTTTCGGCTTCTCTTTTGGTTTTTCCGGTTCTTTCGGTTTTTCAACTTTCGGCGGTTCCGGTTTTACCGTCGGATCATCCAc                                              <  1:751947/251‑1 (MQ=255)                                                                                                                                                                                                                                aaTTCGGATTATTGGTTGTCACCGGACCGGTTGATGTGGCTTTAGAATTCACCGAAGCATTGGAATCTACCGTTTTGTCACCCACAGGCACATCAGTTCTCGGTTTTTGCTTCGGCTTCTCTTTCGGTTTTTCCTTTGGCTTTTCTTTCGGCTTCTCTTTTGGTTTTTCTGGTTCTTTCGGTTTTTCAACTTTCGGCGGTTCCGGTTTTACCGTCGGGTCATCCAc                                              >  1:438031/1‑226 (MQ=255)                                                                                                                                                                                                                                       attattGGTTGTCACCGGACCGGTTGATGTGGCTTTAGAATTCACCGAAGCATTGGAATCTACCGTTTTGTCACCCACAGGCACATCAGTTCTCGGTTTTTGCTTCGGCTTCTCTTTCGGTTTTTCCTTTGGCTTTTCTTTCGGCTTCTCTTTTGGTTTTTCCGGTTCTTTCGGTTTTTCAACTTTCGGCGGTTCCGGTTTTACCGTCGGATCATCCACCGCTTCCTGCTTTTCAGGTTCCGGCGCttttt              <  1:553261/251‑1 (MQ=255)                                                                                                                                                                                                                                         tattGGTTGTCACCGGACCGGTTGATGTGGCTTTAGAATTCACCGAAGCATTGGAATCTACCGttt                                                                                                                                                                                                     >  1:758583/1‑66 (MQ=255)                                                                                                                                                                                                                                               ttGTCACCGGACCGGTTGATGTGGCTTTAGAATTCACCGAAGCATTGGAATCTACCGTTTTGTCACCCGCAGGCACATCAGTTCTCGGTTTTTGCTTCGGCTTCTCTTTCGGTTTTTCCTTTGGCTTTTCTTTCGGCTTCTCTTTTGGTTTTTCCGGTTCTTTCGGTTTTTCAACTTTCGGCGGTTCCGGTTTTACCGTCGGATCATCCACCGCTTCCTGCTTTTCAGGTTCCGGCGCTTTTTCTTCTAc       <  1:735741/250‑1 (MQ=255)                                                                                                                                                                                                                                                   cACCGGACCGGTTGATGTGGCTTTAGAATTCACCGAAGCATTGGAATCTACCGTTTTGTCACCCACAGGCACATCAGTTCTCGGTTTTTGCTTCGGCTTCTCTTTCGGTTTTTCCTTTGGCTTTTCTTTCGGCTTCTCTTTTGGTTTTTCCGGTTCTTTCGGTTTTTCAACTTTCGGCGGTTCCGGTTTTACCGTCGGATCATCCACCGCTTCCTGCTTTTCAGGTTCCGGCGCTTTTTCTTCTACCGGTg  >  1:796369/1‑251 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                       ACCGCACTTAACGCCGCATTATCCAAATCTTCCGCGCCTGAACTTTTTACGATGCTCGCCCCACTAATGGAACCATCTGCACCGATGGAGAAAGAAACGGTCACAATGCCTTGCTTACGCATCATTTTGGCACGTGTTGGGTAGCGTTTATGTTTCTCAATTTCACGTCGAATTGCCGATAAATAGGCATTTCTTTCATCGCTGCTGGCGCCACTGCCTGCCAAATTCGGATTATTGGTTGTCACCAGACCGGTTGATGTGGCTTTAGAATTCACCGAAGCATTGGAATCTACCGTTTTGTCACCCACAGGCACATCAGTTCTCGGTTTTTGCTTCGGCTTCTCTTTCGGTTTTTCCTTTGGCTTTTCTTTCGGCTTCTCTTTTGGTTTTTCCGGTTCTTTCGGTTTTTCAACTTTCGGCGGTTCCGGTTTTACCGTCGGATCATCCACCGCTTCCTGCTTTTCAGGTTCCGGCGCTTTTTCTTCTACCGGTG  >  1/350390‑350882

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑