Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	2,109,648	A→G	I27T (ATC→ACC)	unknown ←	UPF0234 protein Yitk

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	2,109,648	0	A	G	100.0%	244.7 / NA	72	I27T (ATC→ACC)	unknown	UPF0234 protein Yitk
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (42/30);  total (42/30)

TTCTTCGCCATATCGCTTTCAATCCCCTGTTTCAGCTTGATTTCTTTGCTGTAGGTTTTGCCGCTGTGTTCATATTCGGCGGGAATGTCCAGGGAGTTGGATTCAATGTTGCGCTTAATGCAGGCGTTACGCAGAATATCGATAAGTTGTTCCACCTGGAAATCCGATTCGCTGCTGACTTTGATGGTTTCGTTTTTTTCATTAAATTCAATGGCGGCTTGTACGTTGCGGAAGTCCCAGCGGTTGATCAGCTCGCGATTGGCGTTTTCCACGGCGTTGCGGATTTCGTGTCCGCTGATTTCGGAAACAATGTCGAATGATGGCATGGTTTACTCCTTATTTGATTTTGTCGTTAGCACTTAAAATACATAAAAGTGCGGTTAAATCGGTGAAATTGATGATAACTTGCGCTAATTGTTGCACTTTCGGTTTGGCATAATAGGCAACGCCCAAATCGGCGACCTGCATCATGGCAAGATCGTTGG  >  1/2109402‑2109886                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                               ttcttcGCCATATCGCTTTCAATCCCCTGTTTCAGCTTGATTTCTTTGCTGTAGGTTTTGCCGCTGTGTTCATATTCGGCGGGAATGTCCAGGGAGTTGGATTCAATGTTGCGCTTAATGCAGGCGTTACGCAGAATATCGATAAGTTGTTCCACCTGGAAATCCGATTCGCTGCTGACTTTGATGGTTTCGTTTTTTTCATTAAATTCAATGGCGGCTTGTACGTTGCGGAAGTCCCAGCGGTTGGTCAg                                                                                                                                                                                                                                            <  1:658715/251‑1 (MQ=255)                        ccccTGTTTCAGCTTGATTTCTTTGCTGTAGGTTTTGCCGCTGTGTTCATATTCGGCGGGAATGTCCAGGGAGTTGGATTCAATGTTGCGCTTAATGCAGGCGTTACGCAGAATATCGATAAGTTGTTCCACCTGGAAATCCGATTCGCTGCTGACTTTGATGGTTTCGTTTTTTTCATTAAATTCAATGGCGGCTTGTACGTTGCGGAAGTCCCAGCGGTTGGTCAGCTCGCGATTGGCGTTTTCCACgg                                                                                                                                                                                                                     >  1:573736/1‑251 (MQ=255)                             gTTTCAGCTTGATTTCTTTGCTGTAGGTTTTGCCGCTGTGTTCATATTCGGCGGGAATGTCCAGGGAGTTGGATTCAATGTTGCGCTTAATGCAGGCGTTACGCAGAATATCGATAAGTTGTTCCACCTGGAAATCCGATTCGCTGCTGACTTTGATGGTTTCGTTTTTTTCATTAAATTCAATGGCGGCTTGTACGTTGCGGAAGTCCCAGCGGTTGGTCAGCTCGCGATTGGCGTTTTCCACGGCGTTg                                                                                                                                                                                                                >  1:8158/1‑251 (MQ=255)                                                         tttGCCGCTGTGTTCATATTCGGCGGGAATGTCCAGGGAGTTGGATTCAATGTTGCGCTTAATGCAGGCGTTACGCAGAATATCGATAAGTTGTTCCACCTGGAAATCCGATTCGCTGCTGACTTTGATGGTTTCGTTTTTTTCATTAAATTCAATGGCGGCTTGTACGTTGCGGAAGTCCCAGCGGTTGGTCAGCTCGCGATTGGCGTTTTCCACGGCGTTGCGGATTTCGTGTCCGCTGATTTCGGaaa                                                                                                                                                                                    <  1:283023/251‑1 (MQ=255)                                                            gCCGCTGTGTTCATATTCGGCGGGAATGTCCAGGGAGTTGGATTCAATGTTGCGCTTAATGCAGGCGTTACGCAGAATATCGATAAGTTGTTCCACCTGGAAATCCGATTCGCTGCTGACTTTGATGGTTTCGTTTTTTTCATTAAATTCAATGGCGGCTTGTACGTTGCGGAAGTCCCAGCGGTTGGTCAGCTCGCGATTGGCGTTTTCCACGGCGTTGCGGATTTCGTGTCCGCTGATTTCGGAAACaa                                                                                                                                                                                 >  1:712506/1‑251 (MQ=255)                                                                cTGTGTTCATATTCGGCGGGAATGTCCAGGGAGTTGGATTCAATGTTGCGCTTAATGCAGGCGTTACGCAGAATATCGATAAGTTGTTCCACCTGGAAATCCGATTCGCTGCTGACTTTGATGGTTTCGTTTTTTTCATTAAATTCAATGGCGGCTTGTACGTTGCGGAAGTCCCAGCGGTTGGTCAGCTCGCGATTGGCGTTTTCCACGGCGTTGCGGATTTCGTGTCCGCTGATTTCGGAATCAATGt                                                                                                                                                                              <  1:860807/250‑1 (MQ=255)                                                                  gtgtTCATATTCGGCGGGAATGTCCAGGGAGTTGGATTCAATGTTGCGCTTAATGCAGGCGTTACGCAGAATATCGATAAGTTGTTCCACCTGGAAATCCGATTCGCTGCTGACTTTGATGGTTTCGTTTTTTTCATTAAATTCAATGGCGGCTTGTACGTTGCGGAAGTCCCAGCGGTTGGTCAGCTCGCGATTGGCGTTTTCCACGGCGTTGCGGATTTCGTGTCCGCTGATTTCGGAAACAATGTCGa                                                                                                                                                                           >  1:555315/1‑251 (MQ=255)                                                                  gtgtTCATATTCGGCGGGAATGTCCAGGGAGTTGGATTCAATGTTGCGCTTAATGCAGGCGTTACGCAGAATATCGATAAGTTGTTCCACCTGGAAATCCGATTCGCTGCTGACTTTGATGGTTTCGTTTTTTTCATTAAATTCAATGGCGGCTTGTACGTTGCGGAAGTCCCAGCGGTTGGTCAGCTCGCGATTGGCGTTTTCCACGGCGTTGCGGATTTCGTGTCCGCTGATTTCGGAAACAATGTCGa                                                                                                                                                                           >  1:736895/1‑251 (MQ=255)                                                                    gtTCATATTCGGCGGGAATGTCCAGGGAGTTGGATTCAATGTTGCGCTTAATGCAGGCGTTACGCAGAATATCGATAAGTTGTTCCACCTGGAAATCCGATTCGCTGCTGACTTTGATGGTTTCGTTTTTTTCATTAAATTCAATGGCGGCTTGTACGTTGCGGAAGTCCCAGCGGTTGGTCAGCTCGCGATTGGCg                                                                                                                                                                                                                               <  1:772560/197‑1 (MQ=255)                                                                                cggGAATGTCCAGGGAGTTGGATTCAATGTTGCGCTTAATGCAGGCGTTACGCAGAATATCGATAAGTTGTTCCACCTGGAAATCCGATTCGCTGCTGACTTTGATGGTTTCGTTTTTTTCATTAAATTCAATGGCGGCTTGTACGTTGCGGAAGTCCCAGCGGTTGGTCAGCTCGCGATTGGCGTTTTCCACGGCGTTGCGGATTTCGTGTCCGCTGATTTCGGAAACAATGTCGAATGATGGCATGGt                                                                                                                                                              <  1:151310/250‑1 (MQ=255)                                                                                  ggAATGTCCAGGGAGTTGGATTCAATGTTGCGCTTAATGCAGGCGTTACGCAGAATATCGATAAGTTGTTCCACCTGGAAATCCGATTCGCTGCTGACTTTGATGGTTTCGTTTTTTTCATTAAATTCAATGGCGGCTTGTACGTTGCGGAAGTCCCAGCGGTTGGTCAGCTCGCGAt                                                                                                                                                                                                                                    >  1:663079/1‑178 (MQ=255)                                                                                  ggAATGTCCAGGGAGTTGGATTCAATGTTGCGCTTAATGCAGGCGTTACGCAGAATATCGATAAGTTGTTCCACCTGGAAATCCGATTCGCTGCTGACTTTGATGGTTTCGTTTTTTTCATTAAATTCAATGGCGGCTTGTACGTTGCGGAAGTCCCAGCGGTTGGTCAGCTCGCGATTGGCGTTTTCCACGGCGTTGCGGATTTCGTGTCCGCTGATTTCGGAAACAATGTCGAATGATGGCATGGttt                                                                                                                                                            <  1:777473/250‑1 (MQ=255)                                                                                     aTGTCCAGGGAGTTGGATTCAATGTTGCGCTTAATGCAGGCGTTACGCAGAATATCGATAAGTTGTTCCACCTGGAAATCCGATTCGCTGCTGACTTTGATGGTTTCGTTTTTTTCATTAAATTCAATGGCGGCTTGTACGTTGCGGAAGTCCCAGCGGTTGGTCAGCTCGCGATTGGCGTTTTCCACGGCGTTGCGGATTTCGTGTCCGCTGATTTCGGAAACAATGTCGAATGATGGCATGGTTTACt                                                                                                                                                         >  1:194846/1‑250 (MQ=255)                                                                                     aTGTCCAGGGAGTTGGATTCAATGTTGCGCTTAATGCAGGCGTTACGCAGAATATCGATAAGTTGTTCCACCTGGAAATCCGATTCGCTGCTGACTTTGATGGTTTCGTTTTTTTCATTAAATTCAATGGCGGCTTGTACGTTGCGGAAGTCCCAGCGGTTGGTCAGCTCGCGATTGGCGTTTTCCACGGCGTTGCGGATTTCGTGTCCGCTGATTTCGGAAACAATGTCGAATGATGGCATGGTTTACTc                                                                                                                                                        <  1:802926/251‑1 (MQ=255)                                                                                     aTGTACAGGGAGTTGGATTAAATTTTGCGCTTGATGAAGGCGTTAGGCAGAATATTGATAAGTTGTTCCACCTGGAAATTCGATTCGCTGCTGACTTTGATGGTTTCTTTTTTTTCATTAAATTCAATGGCGGCTTGTACGTTGCGGAAGTCCCAGCGGTTGGTCAGCTCGCGATTGGCGTTTTCCACGGCGTTGCGGATTTCGTGTTCGCTGATTTCGGAAACAATGTCGAATGATGGCATGGTTTACTc                                                                                                                                                        <  1:802908/251‑1 (MQ=255)                                                                                      tGTCCAGGGAGTTGGATTCAATGTTGCGCTTAATGCAGGCGTTACGCAGAATATCGATAAGTTGTTCCACCTGGAAATCCGATTCGCTGCTGACTTTGATGGTTTCGTTTTTTTCATTAAATTCAATGGCGGCTTGTACGTTGCGGAAGTCCCAGCGGTTGGTCAGCTCGCGATTGGCGTTTTCCACGGCGTTGCGGATTTCGTGTCCGCTGATTTCGGAAACAATGTCGAATGATGGCATGGTTTACTcc                                                                                                                                                       <  1:302213/251‑1 (MQ=255)                                                                                      tGTCCAGGGAGTTGGATTCAATGTTGCGCTTAATGCAGGCGTTACGCAGAATATCGATAAGTTGTTCCACCTGGAAATCCGATTCGCTGCTGACTTTGATGGTTTCGTTTTTTTCATTAAATTCAATGGCGGCTTGTACGTTGCGGAAGTCCCAGCGGTTGGTCAGCTCGCGATTGGCGTTTTCCACGGCGTTGCGGATTTCGTGTCCGCTGATTTCGGAAACAATGTCGAATGATGGCATGGTTTACTc                                                                                                                                                        >  1:656016/1‑250 (MQ=255)                                                                                       gTCCAGGGAGTTGGATTCAATGTTGCGCTTAATGCAGGCGTTACGCAGAATATCGATAAGTTGTTCCACCTGGAAATCCGATTCGCTGCTGACTTTGATGGTTTCGTTTTTTTCATTAAATTCAATGGCGGCTTGTACGTTGCGGAAGTCCCAGCGGTTGGTCAGCTCGCGATTGGCGTTTTCCACGGCGTTGCGGATTTCGTGTCCGCTGATTTCGGAAACAATGTCGAATGATGGCATGGTTTACTc                                                                                                                                                        >  1:154193/1‑249 (MQ=255)                                                                                       gTCCAGGGAGTTGGATTCAATGTTGCGCTTAATGCAGGCGTTACGCAGAATATCGATAAGTTGTTCCACCTGGAAATCCGATTCGCTGCTGACTTTGATGGTTTCGTTTTTTTCATTAAATTCAATGGCGGCTTGTACGTTGCGGAAGTCCCAGCGGTTGGTCAGCTCGCGATTGGCGTTTTCCACGGCGTTGCGGATTTCGTGTCCGCTGATTTCGGAAACAATGTCGAATGATGGCATGGTTTACTc                                                                                                                                                        >  1:438572/1‑249 (MQ=255)                                                                                       gTCCAGGGAGTTGGATTCAATGTTGCGCTTAATGCAGGCGTTACGCAGAATATCGATAAGTTGTTCCACCTGGAAATCCGATTCGCTGCTGACTTTGATGGTTTCGTTTTTTTCATTAAATTCAATGGCGGCTTGTACGTTGCGGAAGTCCCAGCGGTTGGTCAGCTCGCGATTGGCGTTTTCCACGGCGTTGCGGATTTCGTGTCCGCTGATTTCGGAAACAATGTCGAATGATGGCATGGTTTACTc                                                                                                                                                        >  1:140449/1‑249 (MQ=255)                                                                                              gAGTTGGATTCAATGTTGCGCTTAATGCAGGCGTTACGCAGAATATCGATAAGTTGTTCCACCTGGAAATCCGATTCGCTGCTGACTTTGATGGTTTCGTTTTTTTCATTAAATTCAATGGCGGCTTGTACGTTGCGGAAGTCCCAGCGGTTGGTCAGCTCGCGATTGGCGTTTTCCACGGCGTTGCGGATTTCGTGTCCGCTGATTTCGGAAACAATGTCGAATGATGGCATGGTTTACTCCTTATTTg                                                                                                                                                <  1:330505/250‑1 (MQ=255)                                                                                              gAGTTGGATTCAATGTTGCGCTTAATGCAGGCGTTACGCAGAATATCGATAAGTTGTTCCACCTGGAAATCCGATTCGCTGCTGACTTTGATGGTTTCGTTTTTTTCATTAAATTCAATGGCGGCTTGTACGTTGCGGAAGTCCCAGCGGTTGGTCAGCTCGCGATTGGCGTTTTCCACGGCGTTGCGGATTTCGTGTCCGCTGATTTCGGAAACAATGTCGAATGATGGCATGGTTTACTCCTTATTTGa                                                                                                                                               >  1:642764/1‑251 (MQ=255)                                                                                                gTTGGATTCAATGTTGCGCTTAATGCAGGCGTTACGCAGAATATCGATAAGTTGTTCCACCTGGAAATCCGATTCGCTGCTGACTTTGATGGTTTCGTTTTTTTCATTAAATTCAATGGCGGCTTGTACGTTGCGGAAGTCCCAGCGGTTGGTCAGCTCGCGATTGGCGTTTTCCACGGCGTTGCGGATTTCGTGTCCGCTGATTTCGGAAACAATGTCGAATGATGGCATGGTTTACTcc                                                                                                                                                       <  1:802823/241‑1 (MQ=255)                                                                                                     aTTCAATGTTGCGCTTAATGCAGGCGTTACGCAGAATATCGATAAGTTGTTCCACCTGGAAATCCGATTCGCTGCTGACTTTGATGGTTTCGTTTTTTTCATTAAATTCAATGGCGGCTTGTACGTTGCGGAAGTCCCAGCGGTTGGTCAGCTCGCGATTGGCGTTTTCCACGGCGTTGCGGATTTCGTGTCCGCTGATTTCGGAAACAATGTCGAATGATGGCATGGTTTACTCCTTATTTGATTTTGt                                                                                                                                         >  1:446201/1‑250 (MQ=255)                                                                                                      ttCAATGTTGCGCTTAATGCAGGCGTTACGCAGAATATCGATAAGTTGTTCCACCTGGAAATCCGATTCGCTGCTGACTTTGATGGTTTCGTTTTTTTCATTAAATTCAATGGCGGCTTGTACGTTGCGGAAGTCCCAGCGGTTGGTCAGCTCGCGATTGGCGTTTTCCACGGCGTTGCGGATTTCGTGTCCGCTGATTTCGGAAACAATGTCGAATGATGGCATGGTTTACTCCTTATTTGATTTTGTCg                                                                                                                                       <  1:836032/251‑1 (MQ=255)                                                                                                        cAATGTTGCGCTTAATGCAGGCGTTACGCAGAATATCGATAAGTTGTTCCACCTGGAAATCCGATTCGCTGCTGACTTTGATGGTTTCGTTTTTTTCATTAAATTCAATGGCGGCTTGTACGTTGCGGAAGTCCCAGCGGTTGGTCAGCTCGCGATTGGCGTTTTCCACGGCGTTGCGGATTTCGTGTCCGCTGATTTCGGAAACAATGTCGAATGATGGCATGGTTTACTCCTTATTTGATTTTGTCGtt                                                                                                                                     >  1:699490/1‑251 (MQ=255)                                                                                                          aTGTTGCGCTTAATGCAGGCGTTACGCAGAATATCGATAAGTTGTTCCACCTGGAAATCCGATTCGCTGCTGACTTTGATGGTTTCGTTTTTTTCATTAAATTCAATGGCGGCTTGTACGTTGCGGAAGTCCCAGCGGTTGGTCAGCTCGCGATTGGCGTTTTCCACGGCGTTGCGGATTTCGTGTCCGCTGATTTCGGAAACAATGTCGAATGATGGCATGGTTTACTCCTTATTTGATTTTGTCGTTAg                                                                                                                                   >  1:879607/1‑251 (MQ=255)                                                                                                           tGTTGCGCTTAATGCAGGCGTTACGCAGAATATCGATAAGTTGTTCCACCTGGAAATCCGATTCGCTGCTGACTTTGATGGTTTCGTTTTTTTCATTAAATTCAATGGCGGCTTGTACGTTGCGGAAGTCCCAGCGGTTGGTCAGCTCGCGATTGGCGTTTTCCACGGCGTTGCGGATTTCGTGTCCGCTGATTTCGGAAACAATGTCGAATGATGGCATGGTTTACTCCTTATTTGATTTTGTCGTTAGc                                                                                                                                  <  1:242638/251‑1 (MQ=255)                                                                                                            gTTGCGCTTAATGCAGGCGTTACGCAGAATATCGATAAGTTGTTCCACCTGGAAATCCGATTCGCTGCTGACTTTGATGGTTTCGTTTTTTTCATTAAATTCAATGGCGGCTTGTACGTTGCGGAAGTCCCAGCGGTTGGTCAGCTCGCGATTGGCGTTTTCCACGGCGTTGCGGATTTCGTGTCCGCTGATTTCGGAAACAATGTCGAATGATGGCATGGTTTACTCCTTATTTGAt                                                                                                                                              >  1:509186/1‑238 (MQ=255)                                                                                                                 gcTTAATGCAGGCGTTACGCAGAATATCGATAAGTTGTTCCACCTGGAAATCCGATTCGCTGCTGACTTTGATGGTTTCGTTTTTTTCATTAAATTCAATGGCGGCTTGTACGTTGCGGAAGTCCCAGCGGTTGGTCAGCTCGCGATTGGCGTTTTCCACGGCGTTGCGGATTTCGTGTCCGCTGATTTCGGAAACAATGTCGAATGATGGCATGGTTTACTcc                                                                                                                                                       >  1:86715/1‑224 (MQ=255)                                                                                                                      aTGCAGGCGTTACGCAGAATATCGATAAGTTGTTCCACCTGGAAATCCGATTCGCTGCTGACTTTGATGGTTTCGTTTTTTTCATTAAATTCAATGGCGGCTTGTACGTTGCGGAAGTCCCAGCGGTTGGTCAGCTcg                                                                                                                                                                                                                                        >  1:668835/1‑138 (MQ=255)                                                                                                                      aTGCAGGCGTTACGCAGAATATCGATAAGTTGTTCCACCTGGAAATCCGATTCGCTGCTGACTTTGATGGTTTCGTTTTTTTCATTAAATTCAATGGCGGCTTGTACGTTGCGGAAGTCCCAGCGGTTGGTCAGCTCGCGATTGGCGTTTTcc                                                                                                                                                                                                                         <  1:321769/153‑1 (MQ=255)                                                                                                                      aTGCAGGCGTTACGCAGAATATCGATAAGTTGTTCCACCTGGAAATCCGATTCGCTGCTGACTTTGATGGTTTCGTTTTTTTCATTAAATTCAATGGCGGCTTGTACGTTGCGGAAGTCCCAGCGGTTGGTCAGCTCGCGATTGGCGTTTTCCACGGCGTTGCGGATTTCGTGTCCGCTGATTTCGGAAACAATGTCGAATGATGGCAt                                                                                                                                                                 <  1:808258/209‑1 (MQ=255)                                                                                                                      aTGCAGGCGTTACGCAGAATATCGATAAGTTGTTCCACCTGGAAATCCGATTCGCTGCTGACTTTGATGGTTTCGTTTTTTTCATTAAATTCAATGGCGGCTTGTACGTTGCGGAAGTCCCAGCGGTTGGTCAGCTCGCGATTGGCGTTTTCCACGGCGTTGCGGATTTCGTGTCCGCTGATTTCGGAAACAATGTCGAATGATGGCATGGTTTACTCCTTATTTGATTTTGTCGTTAGCACTTAAAATa                                                                                                                        >  1:129862/1‑250 (MQ=255)                                                                                                                      aTGCAGGCGTTACGCAGAATATCGATAAGTTGTTCCACCTGGAAATCCGATTCGCTGCTGACTTTGATGGTTTCGTTTTTTTCATTAAATTCAATGGCGGCTTGTACGTTGCGGAAGTCCCAGCGGTTGGTCAGCTCGCGATTGGCGTTTTCCACGGCGTTGCGGATTTCGTGTCCGCTGATTTCGGAAACAATGTCGAATGATGGCATGGTTTACTCCTTATTTGATTTTGTCGTTAGCACTTAAAATa                                                                                                                        >  1:675479/1‑250 (MQ=255)                                                                                                                      aTGCAGGCGTTACGCAGAATATCGATAAGTTGTTCCACCTGGAAATCCGATTCGCTGCTGACTTTGATGGTTTCGTTTTTTTCATTAAATTCAATGGCGGCTTGTACGTTGCGGAAGTCCCAGCGGTTGGTCAGCTCGCGATTGGCGTTTTCCACGGCGTTGCGGATTTCGTGTCCGCTGATTTCGGAAACAATGTCGAATGATGGCATGGTTTACTCCTTATTTGATTTTGTCGTTAGCACTTAAAATAc                                                                                                                       <  1:99952/251‑1 (MQ=255)                                                                                                                             cGTTACGCAGAATATCGATAAGTTGTTCCACCTGGAAATCCGATTCGCTGCTGACTTTGATGGTTTCGTTTTTTTCATTAAATTCAATGGCGGCTTGTACGTTGCGGAAGTCCCAGCAGTTGGTCAGCTCGCGATTGGCGTTTTCCACGACGTTGCGGATTTCGTGTCCGCTGATTTCGGAAACAATGTCGAATGATGGCATGGTTTACTCCTTATTTGATTTTGTCGTTAGCAc                                                                                                                                <  1:319442/235‑1 (MQ=255)                                                                                                                                 aCGCAGAATATCGATAAGTTGTTCCACCTGGAAATCCGATTCGCTGCTGACTTTGATGGTTTCGTTTTTTTCATTAAATTCAATGGCGGCTTGTACGTTGCGGAAGTCCCAGCGGTTGGTCAGCTCGCGATTGGCGTTTTCCACGGCGTTGCGGATTTCGTGTCCGCTGATTTCGGAAACAATGTCGAATGATGGCATGGTTTACTCCTTATTTGATTTTGTCGTTAGCACTTAAAATAc                                                                                                                       >  1:306925/1‑240 (MQ=255)                                                                                                                                        atatCGATAAGTTGTTCCACCTGGAAATCCGATTCGCTGCTGACTTTGATGGTTTCGTTTTTTTCATTAAATTCAATGGCGGCTTGTACGTTGCGGAAGTCCCAGCGGTTGGTCAGCTCGCGATTGGCGTTTTCCACGGCGTTGCGGATTTCGTGTCCGCTGATTTCGGGAACAATGTCGAATGATGGCATGGTTTACTCCTTATTTGATTTTGTCGTTAGCACTTAAAATACATAAAAGTGCGGTTAAAt                                                                                                     >  1:424747/1‑251 (MQ=255)                                                                                                                                          atCGATAAGTTGTTCCACCTGGAAATCCGATTCGCTGCTGACTTTGATGGTTTCGTTTTTTTCATTAAATTCAATGGCGGCTTGTACGTTGCGGAAGTCCCAGCGGTTGGTCAGCTCGCGATTGGCGTTTTCCACGGCGTTGCGGATTTCGTGTCCGCTGATTTCGGAAACAATGTCGAATGATGGCATGGTTTACTCCTTATTTGATTTTGTCGTTAGCACTTAAAATACATAAAAGTGCGGTTAAATCg                                                                                                   >  1:577095/1‑251 (MQ=255)                                                                                                                                           tCGATAAGTTGTTCCACCTGGAAATCCGATTCGCTGCTGACTTTGATGGTTTCGTTTTTTTCATTAAATTCAATGGCGGCTTGTACGTTGCGGAAGTCCCAGCGGTTGGTCAGCTCGCGATTGGCGTTTTCCACGGCGTTGCGGATTTCGTGTCCGCTGATTTCGGAAACAATGTCGAATGATGGCATGGTTTACTCCTTATTTGATTTTGTCGTTAGCACTTAAAATACATAAAAGTGCGGTTAAATCgg                                                                                                  >  1:316056/1‑251 (MQ=255)                                                                                                                                           tCGATAAGTTGTTCCACCTGGAAATCCGATTCGCTGCTGACTTTGATGGTTTCGTTTTTTTCATTAAATTCAATGGCGGCTTGTACGTTGCGGAAGTCCCAGCGGTTGGTCAGCTCGCGATTGGCGTTTTCCACGGCGTTGCGGATTTCGTGTCCGCTGATTCCGGAAACAATGTCGAATGATGGCATGGTTTACTCCTTATTTGATTTTGTCGTTAGCACTTAAAATACATAAAAGTGCGGTTAAATCgg                                                                                                  >  1:198797/1‑251 (MQ=255)                                                                                                                                              aTAAGTTGTTCCACCTGGAAATCCGATTCGCTGCTGACTTTGATGGTTTCGTTTTTTTCATTAAATTCAATGGCGGCTTGTACGTTGCGGAAGTCCCAGCGGTTGGTCAGCTCGCGATTGGCGTTTTCCACGGCGTTGCGGATTTCGTGTCCGCTGATTTCGGAAACAATGTCGAATGATGGCATGGTTTACTCCTTATTTGATTTTGTCGTTAGCACTTAAAATACATAAAAGTGCGGTTAAATCGGTg                                                                                                <  1:283226/250‑1 (MQ=255)                                                                                                                                                aaGTTGTTCCACCTGGAAATCCGATTCGCTGCTGACTTTGATGGTTTCGTTTTTTTCATTAAATTCAATGGCGGCTTGTACGTTGCGGAAGTCCCAGCGGTTGGTCAGCTCGCGATTGGCGTTTTCCACGGCGTTGCGGATTTCgtg                                                                                                                                                                                                     >  1:800747/1‑147 (MQ=255)                                                                                                                                                aaGTTGTTCCACCTGGAAATCCGATTCGCTGCTGACTTTGATGGTTTCGTTTTTTTCATTAAATTCAATGGCGGCTTGTACGTTGCGGAAGTCCCAGCGGTTGGTCAGCTCGCGATTGGCGTTTTCCACGGCGTTGCGGATTTCGTGTCCGCTGATTTCGGAAACAATGTCGAATGATGGCATGGTTTACTCCTTATTTGATTTTGTCGTTAGCACTTAAAATACATAAAAGTGCGGTTAAATCGGTGaaa                                                                                             >  1:398492/1‑251 (MQ=255)                                                                                                                                                  gttgttCCACCTGGAAATCCGATTCGCTGCTGACTTTGATGGTTTCGTTTTTTTCATTAAATTCAATGGCGGCTTGTACGTTGCGGAAGTCCCAGCGGTTGGTCAGCTCGCGATTGGCGTTTTCCACGGCGTTGCGGATTTCGTGTCCGCTGATTTCGGAAACAATGTCGAATGATGGCATGGTTTACTCCTTATTTGATTTTGTCGTTAGCACTTAAAATACATAAAAGTGCGGTTAAATCGGTGAAAtt                                                                                           >  1:547941/1‑251 (MQ=255)                                                                                                                                                  gttgttCCACCTGGAAATCCGATTCGCTGCTGACTTTGATGGTTTCGTTTTTTTCATTAAATTCAATGGCGGCTTGTACGTTGCGGAAGTCCCAGCGGTTGGTCAGCTCGCGATTGGCGTTTTCCACGGCGTTGCGGATTTCGTGTCCGCTGATTTCGGAAACAATGTCGAATGATGGCATGGTTTACTCCTTATTTGATTTTGTCGTTAGCACTTAAAATACATAAAAGTGCGGTTAAATCGGTGAAAtt                                                                                           >  1:297469/1‑251 (MQ=255)                                                                                                                                                  gttgttCCACCTGGAAATCCGATTCGCTGCTGACTTTGATGGTTTCGTTTTTTTCATTAAATTCAATGGCGGCTTGTACGTTGCGGAAGTCCCAGCGGTTGGTCAGCTCGCGATTGGCGTTTTCCACGGCGTTGCGGATTTCGTGTCCGCTGATTTCGGAAACAATGTCGAATGATGGCATGGTTTACTCCTTATTTGATTTTGTCGTTAGCACTTAAAATACATAAAAGTGCGGTTAAATCGGTGAAAtt                                                                                           >  1:677011/1‑251 (MQ=255)                                                                                                                                                     gttCCACCTGGAAATCCGATTCGCTGCTGACTTTGATGGTTTCGTTTTTTTCATTAAATTCAATGGCGGCTTGTACGTTGCGGAAGTCCCAGCGGTTGGTCAGCTCGCGATTGGCGTTTTCCACGGCGTTGCGGATTTCGTGTCCGCTGATTTCGGAAACAATGTCGAATGATGGCATGGTTTACTCCTTATTTGATTTTGTCGTTAGCACTTaaaa                                                                                                                          >  1:21131/1‑217 (MQ=255)                                                                                                                                                          aCCTGGAAATCCGATTCGCTGCTGACTTTGATGGTTTCGTTTTTTTCATTAAATTCAATGGCGGCTTGTACGTTGCGGAAGTCCCAGCGGTTGGTCAGCTCGCGATTGGCGTTTTCCACGGCGTTGCGGATTTCGTGTCCGCTGATTTCGGAAACAATGTCGAATGATGGCATGGTTTACTCCTTATTTGATTTTGTCGTTAGCACTTAAAATACATAAAAGTGCGGTTAAATCGGTGAAATTGATGATaa                                                                                   >  1:101030/1‑251 (MQ=255)                                                                                                                                                          aCCTGGAAATCCGATTCGCTGCTGACTTTGATGGTTTCGTTTTTTTCATTAAATTCAATGGCGGCTTGTACGTTGCGGAAGTCCCAGCGGTTGGTCAGCTCGCGATTGGCGTTTTCCACGGCGTTGCGGATTTCGTGTCCGCTGATTTCGGAAACAATGTCGAATGATGGCATGGTTTACTCCTTATTTGATTTTGTCGTTAGCACTTAAAATACATAAAAGTGCGGTTAAATCGGTGAAATTGATGATaa                                                                                   >  1:31472/1‑251 (MQ=255)                                                                                                                                                           ccTGGAAATCCGATTCGCTGCTGACTTTGATGGTTTCGTTTTTTTCATTAAATTCAATGGCGGCTTGTACGTTGCGGAAGTCCCAGCGGTTGGTCAGCTCGCGATTGGCGTTTTCCACGGCGTTGCGGATTTCGTGTCCGCTGATTTCGGAAACAATGTCGAATGATGGCATGGTTTACTCCTTATTTGAt                                                                                                                                              <  1:741216/191‑1 (MQ=255)                                                                                                                                                           ccTGGAAATCCGATTCGCTGCTGACTTTGATGGTTTCGTTTTTTTCATTAAATTCAATGGCGGCTTGTACGTTGCGGAAGTCCCAGCGGTTGGTCAGCTCGCGATTGGCGTTTTCCACGGCGTTGCGGATTTCGTGTCCGCTGATTTCGGAAACAATGTCGAATGATGGCATGGTTTACTCCTTATTTGATTTTGTCGTTAGCACTTAAAATACATAAAAGTGCGGTTAAATCGGTGAAATTGATGATAAc                                                                                  <  1:311071/251‑1 (MQ=255)                                                                                                                                                           ccTGGAAATCCGATTCGCTGCTGACTTTGATGGTTTCGTTTTTTTCATTAAATTCAATGGCGGCTTGTACGTTGCGGAAGTCCCAGCGGTTGGTCAGCTCGCGATTGGCGTTTTCCACGGCGTTGCGGATTTCGTGTCCGCTGATTTCGGAAACAATGTCGAATGATGGCATGGTTTACTCCTTATTTGATTTTGTCGTTAGCACTTAAAATACATAAAAGTGCGGTTAAATCGGTGAAATTGATGATAAc                                                                                  <  1:163121/251‑1 (MQ=255)                                                                                                                                                                  aTCCGATTCGCTGCTGACTTTGATGGTTTCGTTTTTTTCATTAAATTCAATGGCGGCTTGTACGTTGCGGAAGTCCCAGCGGTTGGTCAGCTCGCGATTGGCGTTTTCCACGGCGTTGCGGATTTCGTGTCCGCTGATTTCGGAAACAATGTCGAATGATGGCATGGTTTACTCCTTATTTGATTTTGTCGTTAGCACTTAAAATACATAAAAg                                                                                                                >  1:194856/1‑214 (MQ=255)                                                                                                                                                                       aTTCGCTGCTGACTTTGATGGTTTCGTTTTTTTCATTAAATTCAATGGCGGCTTGTACGTTGCGGAAGTCCCAGCGGTTGGTCAGCTCGCGATTGGCGTTTTCCACGGCGTTGCGGATTTCGTGTCCGCTGATTTCGGAAACAATGTCGAATGATGGCATGGTTTACTCCTTATTTGATTTTGTCGTTAGCACTTAAAATACATAAAAGTGCGGTTAAATCGGTGAAATTGATGATAACTTGCGCTAAttg                                                                      <  1:118867/251‑1 (MQ=255)                                                                                                                                                                          cGCTGCTGACTTTGATGGTTTCGTTTTTTTCATTAAATTCAATGGCGGCTTGTACGTTGCGGAAGTCCCAGCGGTTGGTCAGCTCGCGATTGGCGTTTTCCACGGCGTTGCGGATTTCGTGTCCGCTGATTTCGGAAACAATGTCGAATGATGGCATGGTTTACTCCTTATTTGATTTTGTCGTTAGCACTTAAAATACATAAAAGTGCGGTTAAATc                                                                                                    >  1:655500/1‑218 (MQ=255)                                                                                                                                                                                    tttGATGGTTTCGTTTTTTTCATTAAATTCAATGGCGGCTTGTACGTTGCGGAAGTCCCAGCGGTTGGTCAGCTCGCGATTGGCGTTTTCCACGGCGTTGCGGATTTCGTGTCCGCTGATTTCGGAAACAATGTCGAATGATGGCATGGTTTACTCCTTATTTGATTTTGTCGTTAGCACTTAAAATACATAAAAGTGCGGTTAAATCGGTGAAATTGATGATAACTTGCGCTAATTGTTGCACTTTCGGt                                                         <  1:827057/251‑1 (MQ=255)                                                                                                                                                                                    tttGATGGTTTCGTTTTTTTCATTAAATTCAATGGCGGCTTGTACGTTGCGGAAGTCCCAGCGGTTGGTCAGCTCGCGATTGGCGTTTTCCACGGCGTTGCGGATTTCGTGTCCGCTGATTTCGGAAACAATGTCGAATGATGGCATGGTTTACTCCTTATTTGATTTTGTCGTTAGCACTTAAAATACATAAAAGTGCGGTTAAATCGGTGAAATTGATGATAACTTGCGCTAATTGTTGCACTTTCGGt                                                         <  1:825536/251‑1 (MQ=255)                                                                                                                                                                                                       tCATTAAATTCAATGGCGGCTTGTACGTTGCGGAAGTCCCAGCGGTTGGTCAGCTCGCGATTGGCGTTTTCCACGGCGTTGCGGATTTCGTGTCCGCTGATTTCGGAAACAATGTCGAATGATGGCATGGTTTACTCCTTATTTGATTTTGTCGTTAGCACTTAAAATACATAAAAg                                                                                                                <  1:488764/177‑1 (MQ=255)                                                                                                                                                                                                            aaaTTCAATGGCGGCTTGTACGTTGCGGAAGTCCCAGCGGTTGGTCAGCTCGCGATTGGCGTTTTCCACGGCGTTGCGGATTTCGTGTCCGCTGATTTCGGAAACAATGTCGAATGATGGCATGGTTTACTCCTTATTTGATTTTGTCGTTAGCACTTAAAATACATAAAAGTGCGGTTAAATCGGTGAAATTGATGATAACTTGCGCTAATTGTTGCACTTTCGGTTTGGCATAATAGGCAACGCCCaaa                                 >  1:588769/1‑251 (MQ=255)                                                                                                                                                                                                              aTTCAATGGCGGCTTGTACGTTGCGGAAGTCCCAGCGGTTGGTCAGCTCGCGATTGGCGTTTTCCACGGCGTTGCGGATTTCGTGTCCGCTGATTTCGGAAACAATGTCGAATGATGGCATGGTTTACTCCTTATTTGATTTTGTCGTTAGCACTTAAAATACATAAAAGTGCGGTTAAATCGGTGAAATTGATGATAACTTGCGCTAATTGTTGCACTTTCGGTTTGGCATAATAg                                             <  1:177954/237‑1 (MQ=255)                                                                                                                                                                                                              aTTCAATGGCGGCTTGTACGTTGCGGAAGTCCCAGCGGTTGGTCAGCTCGCGATTGGCGTTTTCCACGGCGTTGCGGATTTCGTGTCCGCTGATTTCGGAAACAATGTCGAATGATGGCATGGTTTACTCCTTATTTGATTTTGTCGTTAGCACTTAAAATACATAAAAGTGCGGTTAAATCGGTGAAATTGATGATAACTTGCGCTAATTGTTGCACTTTCGGTTTGGCATAATAGGCAACGCCCAAAt                                >  1:670915/1‑250 (MQ=255)                                                                                                                                                                                                                        ggcTTGTACGTTGCGGAAGTCCCAGCGGTTGGTCAGCTCGCGATTGGCGTTTTCCACGGCGTTGCGGATTTCGTGTCCGCTGATTTCGGAAACAATGTCGAATGATGGCATGGTTTACTCCTTATTTGATTTTGTCGTTAGCACTTAAAATACATAAAAGTGCGGTTAAATCGGTGAAATTGATGATAACTTGCGCTAATTGTTGCACTTTCGGTTTGGCATAATAGGCAACGCGCAAATCGGCGACCTg                      >  1:642651/1‑250 (MQ=255)                                                                                                                                                                                                                        ggcTTGTACGTTGCGGAAGTCCCAGCGGTTGGTCAGCTCGCGATTGGCGTTTTCCACGGCGTTGCGGATTTCGTGTCCGCTGATTTCGGAAACAATGTCGAATGATGGCATGGTTTACTCCTTATTTGATTTTGTCGTTAGCACTTAAAATACATAAAAGTGCGGTTAAATCGGTGAAATTGATGATAACTTGCGCTAATTGTTGCACTTTCGGTTTGGCATAATAGGCAACGCCCAAATCGGCGACCTg                      >  1:121103/1‑250 (MQ=255)                                                                                                                                                                                                                         gcTTGTACGTTGCGGAAGTCCCAGCGGTTGGTCAGCTCGCGATTGGCGTTTTCCACGGCGTTGCGGATTTCGTGTCCGCTGATTTCGGAAACAATGTCGAATGATGGCATGGTTTACTCCTTATTTGATTTTGTCGTTAGCACTTAAAATACATAAAAGTGCGGTTAAATCGGTGAAATTGATGATAACTTGCGCTAATTGTTGCACTTTCGGTTTGGCATAATAGGCAACGCCCAAATCGGCGACCTggg                    >  1:569973/1‑249 (MQ=255)                                                                                                                                                                                                                          cTTGTACGTTGCGGAAGTCCCAGCGGTTGGTCAGCTCGCGATTGGCGTTTTCCACGGCGTTGCGGATTTCGTGTCCGCTGATTTCGGAAACAATGTCGAATGATGGCATGGTTTACTCCTTATTTGATTTTGTCGTTAGCACTTAAAATACATAAAAGTGCGGTTAAATCGGTGAAATTGATGATAACTTGCGCTAATTGTTGCACTTTCGGTTTGGCATAATAGGCAACGCCCAAATCGGCGACCTGcat                   <  1:297347/251‑1 (MQ=255)                                                                                                                                                                                                                            tGTACGTTGCGGAAGTCCCAGCGGTTGGTCAGCTCGCGATTGGCGTTTTCCACGGCGTTGCGGATTTCGTGTCCGCTGATTTCGGAAACAATGTCGAATGATGGCATGGTTTACTCCTTATTTGATTTTGTCGTTAGCACTTAAAATACATAAAAGTGCGGTTAAATCGGTGAAATTGATGATAACTTGCGCTAATTGTTGCACTTTCGGTTTGGCATAATAGGCAACGCCCAAATCGGCGACCTGcatca                 <  1:690014/251‑1 (MQ=255)                                                                                                                                                                                                                                cGTTGCGGAAGTCCCAGCGGTTGGTCAGCTCGCGATTGGCGTTTTCCACGGCGTTGCGGATTTCGTGTCCGCTGATTTCGGAAACAATGTCGAATGATGGCATGGTTTACTCCTTATTTGATTTTGTCGTTAGCACTTAAAATACATAAAAGTGCGGTTAAATCGGTGAAATTGATGATAACTTGCGCTAATTGTTGCACTTTCGGTTTGGCATAATAGGCAACGCCCAAATCGGCGACCTGCATCATgg              >  1:801861/1‑250 (MQ=255)                                                                                                                                                                                                                                  ttGCGGAAGTCCCAGCGGTTGGTCAGCTCGCGATTGGCGTTTTCCACGGCGTTGCGGATTTCGTGTCCGCTGATTTCGGAAACAATGTCGAATGATGGCATGGTTTACTCCTTATTTGATTTTGTCGTTAGCACTTAAAATACATAAAAGTGCGGTTAAATCGGTGAAATTGATGATAACTTGCGCTAATTGTTGCACTTTCGGtt                                                        <  1:185938/206‑1 (MQ=255)                                                                                                                                                                                                                                          gTCCCAGCGGTTGGTCAGCTCGCGATTGGCGTTTTCCACGGCGTTGCGGATTTCGTGTCCGCTGATTTCGGAAACAATGTCGAATGATGGCATGGTTTACTCCTTATTTGATTTTGTCGTTAGCACTTAAAATACATAAAAg                                                                                                                <  1:496071/142‑1 (MQ=255)                                                                                                                                                                                                                                            cccAGCGGTTGGTCAGCTCGCGATTGGCGTTTTCCACGGCGTTGCGGATTTCGTGTCCGCTGATTTCGGAAACAATGTCGAATGATGGCATGGTTTACTCCTTATTTGATTTTGTCGTTAGCACTTAAAATACATAAAAGTGCGGTTAAATCGGTGAAATTGATGATAACTTGCGCTAATTGTTGCACTTTCGGTTTGGCATAATAGGCAACGCCCAAATCGGCGACCTGCATCATGGCAAGATCGTTgg  >  1:287509/1‑250 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                               TTCTTCGCCATATCGCTTTCAATCCCCTGTTTCAGCTTGATTTCTTTGCTGTAGGTTTTGCCGCTGTGTTCATATTCGGCGGGAATGTCCAGGGAGTTGGATTCAATGTTGCGCTTAATGCAGGCGTTACGCAGAATATCGATAAGTTGTTCCACCTGGAAATCCGATTCGCTGCTGACTTTGATGGTTTCGTTTTTTTCATTAAATTCAATGGCGGCTTGTACGTTGCGGAAGTCCCAGCGGTTGATCAGCTCGCGATTGGCGTTTTCCACGGCGTTGCGGATTTCGTGTCCGCTGATTTCGGAAACAATGTCGAATGATGGCATGGTTTACTCCTTATTTGATTTTGTCGTTAGCACTTAAAATACATAAAAGTGCGGTTAAATCGGTGAAATTGATGATAACTTGCGCTAATTGTTGCACTTTCGGTTTGGCATAATAGGCAACGCCCAAATCGGCGACCTGCATCATGGCAAGATCGTTGG  >  1/2109402‑2109886

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑