Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	2,092,109	T→C	intergenic (‑58/+209)	unknown ← / ← unknown	Ribonuclease E (EC 3.1.26.12)/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	2,092,109	0	T	C	100.0%	235.8 / NA	69	intergenic (‑58/+209)	unknown/unknown	Ribonuclease E (EC 3.1.26.12)/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (36/33);  total (36/33)

CTGCACCGTAATCCACAAAAGCGGCTTCCAAACTCGGCTCAACCCGAGTAATTTTTCCCTTGTAAATATTGGCTTTTTTCTGTTCATGCCCCAGACTCTCAATGTCCAGGTCGAATAAGCGTTGCCCATCGACTAACGCAACGCGCAACTCTTCTTTTTGAGTCGCATTGATTAACATTCTTTTCATTCGTTTTCTCTTTTATAAAAAAGGGCGAACCCTAAAAAACTCCATCAAAATGGACCGTACTTTGCTTAGTAATCGACCTCGTGTCTGTCGCAGAATGTCCGTCTTCCCGACCGTCATCTGCTGGGTGCATTGACTACCTATTAAAATCAATCGAATGTCGAAATTTTAATAAAACAAAACCTGTTAGAAAAAATGGCCTGCAAGCCGACGTTCATTTTGACGCTAAAAAATCATTGACCTATCAATGTCTTATGCCGAATGCTGCATTATATCCCGTCAATGATAACGTATTTAT  >  1/2091865‑2092346                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                              cTGCACCGTAATCCACAAAAGCGGCTTCCAAACTCGGCTCAACCCGAGTAATTTTTCCCTTGTAAATATTGGCTTTTTTCTGTTCATGCCCCAGACTCTCAATGTCCAGGTCGAATAAGCGTTGCCCATCGACTAACGCAACGCGCAACTCTTCTTTTTGAGTCGCATTGATTAACATTCTTTTCATTCGTTTTCTCTTTTATAAAAAAGGGCGAACCCTAAAAAACTCCATCAAAATGGACCGCACTTTg                                                                                                                                                                                                                                         >  1:552560/1‑251 (MQ=255)    cACCGTAATCCACAAAAGCGGCTTCCAAACTCGGCTCAACCCGAGTAATTTTTCCCTTGTAAATATTGGCTTTTTTCTGTTCATGCCCCAGACTCTCAATGTCCAGGTCGAATAAGCGTTGCCCATCGACTAACGCAACGCGCAACTCTTCTTTTTGAGTCGCATTGATTAACATTCTTTTCATTCGTTTTCTCTTTTATAAAAAAGGGCGAACCCTAAAAAACTCCATCAAAATGGACCGCACTTTGCtt                                                                                                                                                                                                                                      <  1:334974/251‑1 (MQ=255)       cGTAATCCACAAAAGCGGCTTCCAAACTCGGCTCAACCCGAGTAATTTTTCCCTTGTAAATATTGGCTTTTTTCTGTTCATGCCCCAGACTCTCAATGTCCAGGTCGAATAAGCGTTGCCCATCGACTAACGCAACGCGCAACTCTTCTTTTTGAGTCGCATTGATTAACATTCTTTTCATTCGTTTTCTCTTTTATAAAAAAGGGCGAACCCTAAAAAACTCCATCAAAATGGACCGCACTTTGCTTAGt                                                                                                                                                                                                                                   <  1:732767/251‑1 (MQ=255)        gTAATCCACAAAAGCGGCTTCCAAACTCGGCTCAACCCGAGTAATTTTTCCCTTGTAAATATTGGCTTTTTTCTGTTCATGCCCCAGACTCTCAATGTCCAGGTCGAATAAGCGTTGCCCATCGACTAACGCAACGCGCAACTCTTCTTTTTGAGTCGCATTGATTAACATTCTTTTCATTCGTTTTCTCTTTTATAAAAAAGGGCGAACCCTAAAAAACTCCATCAAAATGGACCGCACTTTGCTTAGTa                                                                                                                                                                                                                                  >  1:388828/1‑251 (MQ=255)         tAATCCACAAAAGCGGCTTCCAAACTCGGCTCAACCCGAGTAATTTTTCCCTTGTAAATATTGGCTTTTTTCTGTTCATGCCCCAGACTCTCAATGTCCAGGTCGAATAAGCGTTGCCCATCGACTAACGCAACGCGCAACTCTTCTTTTTGAGTCGCATTGATTAACATTCTTTTCATTCGTTTTCTCTTTTATAAAAAAGGGCGAACCCTAAAAAACTCCATCAAAATGGACCGCACTTTGCTTAGTaa                                                                                                                                                                                                                                 <  1:129080/251‑1 (MQ=255)           aTCCACAAAAGCGGCTTCCAAACTCGGCTCAACCCGAGTAATTTTTCCCTTGTAAATATTGGCTTTTTTCTGTTCATGCCCCAGACTCTCAATGTCCAGGTCGAATAAGCGTTGCCCATCGACTAACGCAACGCGCAACTCTTCTTTTTGAGTCGCATTGATTAACATTCTTTTCATTCGTTTTCTCTTTTATAAAAAAGGGCGAACCCTAAAAAACTCCATCAAAATGGACCGCACTTTGCTTAGTAAt                                                                                                                                                                                                                                >  1:27067/1‑250 (MQ=255)             ccACAAAAGCGGCTTCCAAACTCGGCTCAACCCGAGTAATTTTTCCCTTGTAAATATTGGCTTTTTTCTGTTCATGCCCCAGACTCTCAATGTCCAGGTCGAATAAGCGTTGCCCATCGACTAACGCAACGCGCAACTCTTCTTTTTGAGTCGCATTGATTAACATTCTTTTCATTCGTTTTCTCTTTTATAAAAAAGGGCGAACCCTAAAAAACTCCATCAAAATGGACCGCACTTTGCTTAGt                                                                                                                                                                                                                                   >  1:3935/1‑245 (MQ=255)              cacaAAAGCGGCTTCCAAACTCGGCTCAACCCGAGTAATTTTTCCCTTGTAAATATTGGCTTTTTTCTGTTCATGCCCCAGACTCTCAATGTCCAGGTCGAATAAGCGTTGCCCATCGACTAACGCAACGCGCAACTCTTCTTTTTGAGTCGCATTGATTAACATTCTTTTCATTCGTTTTCTCTTTTATAAAAAAGGGCGAACCCTAAAAAACTCCATCAAAATGGACCGCACTTTGCTTAGTAATCGAc                                                                                                                                                                                                                            <  1:66845/251‑1 (MQ=255)                      cGGCTTCCAAACTCGGCTCAACCCGAGTAATTTTTCCCTTGTAAATATTGGCTTTTTTCTGTTCATGCCCCAGACTCTCAATGTCCAGGTCGAATAAGCGTTGCCCATCGACTAACGCAACGCGCAACTCTTCTTTTTGAGTCGCATTGATTAACATTCTTTTCATTCGTTTTCTCTTTTATAAAAAAGGGCGAACCCTAAAAAACTCCATCAAAATGGACCGCACTTTGCTTAGTAATCGACCTCgtg                                                                                                                                                                                                                      <  1:539706/249‑1 (MQ=255)                         cTTCCAAACTCGGCTCAACCCGAGTAATTTTTCCCTTGTAAATATTGGCTTTTTTCTGTTCATGCCCCAGACTCTCAATGTCCAGGTCGAATAAGCGTTGCCCATCGACTAACGCAACGCGCAACTCTTCTTTTTGAGTCGCATTGATTAACATTCTTTTCATTCGTTTTCTCTTTTATAAAAAAGGGCGAACCCTAAAAAACTCCATCAAAATGGACCGCACTTTGCTTAGTAATCGACCTCgtgt                                                                                                                                                                                                                     >  1:404468/1‑247 (MQ=255)                                aCTCGGCTCAACCCGAGTAATTTTTCCCTTGTAAATATTGGCTTTTTTCTGTTCATGCCCCAGACTCTCAATGTCCAGGTCGAATAAGCGTTGCCCATCGACTAACGCAACGCGCAACTCTTCTTTTTGAGTCGCATTGATTAACATTCTTTTCATTCGTTTTCTCTTTTATAAAAAAGGGCGAACCCTAAAAAACTCCATCAAAATGGACCGCACTTTGCTTAGTAATCGAcc                                                                                                                                                                                                                           >  1:879399/1‑234 (MQ=255)                                aCTCGGCTCAACCCGAGTAATTTTTCCCTTGTAAATATTGGCTTTTTTCTGTTCATGCCCCAGACTCTCAATGTCCAGGTCGAATAAGCGTTGCCCATCGACTAACGCAACGCGCAACTCTTCTTTTTGAGTCGCATTGATTAACATTCTTTTCATTCGTTTTCTCTTTTATAAAAAAGGGCGAACCCTAAAAAACTCCATCAAAATGGACCGCACTTTGCTTAGTAATCGACCTCGTGTCTGTCGCAGaa                                                                                                                                                                                                          <  1:640494/251‑1 (MQ=255)                                     gCTCAACCCGAGTAATTTTTCCCTTGTAAATATTGGCTTTTTTCTGTTCATGCCCCAGACTCTCAATGTCCAGGTCGAATAAGCGTTGCCCATCGACTAACGCAACGCGCAACTCTTCTTTTTGAGTCGCATTGATTAACATTCTTTTCATTCGTTTTCTCTTTTATAAAAAAGGGCGAACCCTAAAAAACTCCATCAAAATGGACCGCACTTTGCTTAGTAATCGACCTCGtgtc                                                                                                                                                                                                                    >  1:233055/1‑236 (MQ=255)                                         aaCCCGAGTAATTTTTCCCTTGTAAATATTGGCTTTTTTCTGTTCATGCCCCAGACTCTCAATGTCCAGGTCGAATAAGCGTTGCCCATCGACTAACGCAACGCGCAACTCTTCTTTTTGAGTCGCATTGATTAACATTCTTTTCATTCGTTTTCTCTTTTATAAAAAAGGGCGAACCCTAAAAAACTCCATCAAAATGGACCGCACTTTGCTTAGTAATCGACCTCGTGTCTGTCGCAGAATGTCCGt                                                                                                                                                                                                   >  1:842100/1‑249 (MQ=255)                                                           cTTGTAAATATTGGCTTTTTTCTGTTCATGCCCCAGACTCTCAATGTCCAGGTCGAATAAGCGTTGCCCATCGACTAACGCAACGCGCAACTCTTCTTTTTGAGTCGCATTGATTAACATTCTTTTCATTCGTTTTCTCTTTTATAAAAAAGGGCGAACCCTAAAAAACTCCATCAAAATGGACCGCACTTTGCTTAGTAATCGACCTCGTGTCTGTCGCAGAATGTCCGTCTTCCCGACCGTCATctgc                                                                                                                                                                                >  1:883541/1‑250 (MQ=255)                                                           cTTGTAAATATTGGCTTTTTTCTGTTCATGCCCCAGACTCTCAATGTCCAGGTCGAATAAGCGTTGCCCATCGACTAACGCAACGCGCAACTCTTCTTTTTGAGTCGCATTGATTAACATTCTTTTCATTCGTTTTCTCTTTTATAAAAAAGGGCGAACCCTAAAAAACTCCATCAAAATGGACCGCACTTTGCTTAGTAATCGACCTCGTGTCTGTCGCAGAATGTCCGTCTTCCCGACCGTCATctg                                                                                                                                                                                 >  1:370473/1‑249 (MQ=255)                                                             tGTAAATATTGGCTTTTTTCTGTTCATGCCCCAGACTCTCAATGTCCAGGTCGAATAAGCGTTGCCCATCGACTAACGCAACGCGCAACTCTTCTTTTTGAGTCGCATTGATTAACATTCTTTTCATTCGTTTTCTCTTTTATAAAAAAGGGCGAACCCTAAAAAACTCCATCAAAATGGACCGCACTTTGCTTAGTAATCGACCTCGTGTCTGTCGCAGAATGTCCGTCTTCCCGACCGTCATCTGCTgg                                                                                                                                                                             >  1:621344/1‑251 (MQ=255)                                                                        gCTTTTTTCTGTTCATGCCCCAGACTCTCAATGTCCAGGTCGAATAAGCGTTGCCCATCGACTAACGCAACGCGCAACTCTTCTTTTTGAGTCGCATTGATTAACATTCTTTTCATTCGTTTTCTCTTTTATAAAAAAGGGCGAACCCTAAAAAACTCCATCAAAATGGACCGCACTTTGCTTAGTAATCGACCTCGTGTCTGTCGCAGAATGTCCGTCTTCCCGACCGTCATc                                                                                                                                                                                   >  1:136370/1‑234 (MQ=255)                                                                        gCTTTTTTCTGTTCATGCCCCAGACTCTCAATGTCCAGGTCGAATAAGCGTTGCCCATCGACTAACGCAACGCGCAACTCTTCTTTTTGAGTCGCATTGATTAACATTCTTTTCATTCGTTTTCTCTTTTATAAAAAAGGGCGAACCCTAAAAAACTCCATCAAAATGGACCGCACTTTGCTTAGTAATCGACCTCGTGTCTGTCGCAGAATGTCCGTCTTCCCGACCGTCATCTGCTGGGTGCATTGAc                                                                                                                                                                   <  1:649041/250‑1 (MQ=255)                                                                                 tGTTCATGCCCCAGACTCTCAATGTCCAGGTCGAATAAGCGTTGCCCATCGACTAACGCAACGCGCAACTCTTCTTTTTGAGTCGCATTGATTAACATTCTTTTCATTCGTTTTCTCTTTTATAAAAAAGGGCGAACCCTAAAAAACTCCATCAAAATGGACCGCACTTTGCTTAGTAATCGACCTCGTGTCTGTCGCAGAATGTCCGTCTTCCCGACCGTCATCTGCTGGGTGCATTGACTACCTATTaa                                                                                                                                                         <  1:583192/251‑1 (MQ=255)                                                                                  gTTCATGCCCCAGACTCTCAATGTCCAGGTCGAATAAGCGTTGCCCATCGACTAACGCAACGCGCAACTCTTCTTTTTGAGTCGCATTGATTAACATTCTTTTCATTCGTTTTCTCTTTTATAAAAAAGGGCGAACCCTAAAAAACTCCATCAAAATGGACCGCACTTTGCTTAGTAATCGACCTCGTGTCTGTCGCAGaa                                                                                                                                                                                                          >  1:753650/1‑201 (MQ=255)                                                                                  gTTCATGCCCCAGACTCTCAATGTCCAGGTCGAATAAGCGTTGCCCATCGACTAACGCAACGCGCAACTCTTCTTTTTGAGTCGCATTGATTAACATTCTTTTCATTCGTTTTCTCTTTTATAAAAAAGGGCGAACCCTAAAAAACTCCATCAAAATGGACCGCACTTTGCTTAGTAATCGACCTCGTGTCTGTCGCAGAATGTCCGTCTTCCCGACCGTCATCTGCTGGGTGCATTGACTACCTATTaaa                                                                                                                                                        >  1:796329/1‑251 (MQ=255)                                                                                     cATGCCCCAGACTCTCAATGTCCAGGTCGAATAAGCGTTGCCCATCGACTAACGCAACGCGCAACTCTTCTTTTTGAGTCGCATTGATTAACATTCTTTTCATTCGTTTTCTCTTTTATAAAAAAGGGCGAACCCTAAAAAACTCCATCAAAATGGACCGCACTTTGCTTAGTAATCGACCTCGTGTCTGTCGCAGAATGTCCGTCTTCCCGACCGTCATCTGCTGGGTGCATTGACTACCTATTAAaatc                                                                                                                                                     <  1:667359/251‑1 (MQ=255)                                                                                        gCCCCAGACTCTCAATGTCCAGGTCGAATAAGCGTTGCCCATCGACTAACGCAACGCGCAACTCTTCTTTTTGAGTCGCATTGATTAACATTCTTTTCATTCGTTTTCTCTTTTATAAAAAAGGGCGAACCCTAAAAAACTCCATCAAAATGGACCGCACTTTGCTTAGTAATCGACCTCGTGTCTGTCGCAGaa                                                                                                                                                                                                          >  1:578992/1‑195 (MQ=255)                                                                                        gCCCCAGACTCTCAATGTCCAGGTCGAATAAGCGTTGCCCATCGACTAACGCAACGCGCAACTCTTCTTTTTGAGTCGCATTGATTAACATTCTTTTCATTCGTTTTCTCTTTTATAAAAAAGGGCGAACCCTAAAAAACTCCATCAAAATGGACCGCACTTTGCTTAGTAATCGACCTCGTGTCTGTCGCAGAATGTCCGTCTTCCCGACCGTCATctgc                                                                                                                                                                                >  1:423330/1‑221 (MQ=255)                                                                                        gCCCCAGACTCTCAATGTCCAGGTCGAATAAGCGTTGCCCATCGACTAACGCAACGCGCAACTCTTCTTTTTGAGTCGCATTGATTAACATTCTTTTCATTCGTTTTCTCTTTTATAAAAAAGGGCGAACCCTAAAAAACTCCATCAAAATGGACCGCACTTTGCTTAGTAATCGACCTCGTGTCTGTCGCAGAATGTCCGTCTTCCCGACCGTCATCTGCTGGGTGCATTGACTACCTATTAAaatcaat                                                                                                                                                  >  1:86542/1‑251 (MQ=255)                                                                                        gCCCCAGACTCTCAATGTCCAGGTCGAATAAGCGTTGCCCATCGACTAACGCAACGCGCAACTCTTCTTTTTGAGTCGCATTGATTAACATTCTTTTCATTCGTTTTCTCTTTTATAAAAAAGGGCGAACCCTAAAAAACTCCATCAAAATGGACCGCACTTTGCTTAGTAATCGACCTCGTGTCTGTCGCAGAATGTCCGTCTTCCCGACCGTCATCTGCTGGGTGCATTGACTACCTATTAAaat                                                                                                                                                      <  1:584530/247‑1 (MQ=255)                                                                                         ccccAGACTCTCAATGTCCAGGTCGAATAAGCGTTGCCCATCGACTAACGCAACGCGCAACTCTTCTTTTTGAGTCGCATTGATTAACATTCTTTTCATTCGTTTTCTCTTTTATAAAAAAGGGCGAACCCTAAAAAACTCCATCAAAATGGACCGCACTTTGCTTAGTAATCGACCTCGTGTCTGTCGCAGAATGTCCGTCTTCCCGACCGTCATCTGCTGGGTGCATTGACTACCTATTAAaatcaat                                                                                                                                                  >  1:341696/1‑250 (MQ=255)                                                                                         ccccAGACTCTCAATGTCCAGGTCGAATAAGCGTTGCCCATCGACTAACGCAACGCGCAACTCTTCTTTTTGAGTCGCATTGATTAACATTCTTTTCATTCGTTTTCTCTTTTATAAAAAAGGGCGAACCCTAAAAAACTCCATCAAAATGGACCGCACTTTGCTTAGTAATCGACCTCGTGTCTGTCGCAGAATGTCCGTCTTCCCGACCGTCATCTGCTGGGTGCATTGACTACCTATTAAaatcaat                                                                                                                                                  >  1:705425/1‑250 (MQ=255)                                                                                               aCTCTCAATGTCCAGGTCGAATAAGCGTTGCCCATCGACTAACGCAACGCGCAACTCTTCTTTTTGAGTCGCATTGATTAACATTCTTTTCATTCGTTTTCTCTTTTATAAAAAAGGGCGAACCCTAAAAAACTCCATCAAAATGGACCGCACTTTGCTTAGTAATCGACCTCGTGTCTGTCGCAGAATGTCCGTCTTCCCGACCGTCATCTGCTGGGTGCATTGACTACCTATTAAAATCAATCGAAt                                                                                                                                             <  1:559059/249‑1 (MQ=255)                                                                                                ctctCAATGTCCAGGTCGAATAAGCGTTGCCCATCGACTAACGCAACGCGCAACTCTTCTTTTTGAGTCGCATTGATTAACATTCTTTTCATTCGTTTTCTCTTTTATAAAAAAGGGCGAACCCTAAAAAACTCCATCAAAATGGACCGCACTTTGCTTAGTAATCGACCTCGTGTCTGTCGCAGAATGTCCGTCTTCCCGACCGTCATCTGCTggg                                                                                                                                                                            <  1:157326/217‑1 (MQ=255)                                                                                                ctctCAATGTCCAGGTCGAATAAGCGTTGCCCATCGACTAACGCAACGCGCAACTCTTCTTTTTGAGTCGCATTGATTAACATTCTTTTCATTCGTTTTCTCTTTTATAAAAAAGGGCGAACCCTAAAAAACTCCATCAAAATGGACCGCACTTTGCTTAGTAATCGACCTCGTGTCTGTCGCAGAATGTCCGTCTTCCCGACCGTCATCTGCTGGGTGCATTGACTACCTATTAAaatc                                                                                                                                                     <  1:460947/240‑1 (MQ=255)                                                                                                ctctCAATGTCCAGGTCGAATAAGCGTTGCCCATCGACTAACGCAACGCGCAACTCTTCTTTTTGAGTCGCATTGATTAACATTCTTTTCATTCGTTTTCTCTTTTATAAAAAAGGGCGAACCCTAAAAAACTCCATCAAAATGGACCGCACTTTGCTTAGTAATCGACCTCGTGTCTGTCGCAGAATGTCCGTCTTCCCGACCGTCATCTGCTGGGTGCATTGACTACCTATTAAAATCAATCGAATGt                                                                                                                                           >  1:785335/1‑250 (MQ=255)                                                                                                  ctcAATGTCCAGGTCGAATAAGCGTTGCCCATCGACTAACGCAACGCGCAACTCTTCTTTTTGAGTCGCATTGATTAACATTCTTTTCATTCGTTTTCTCTTTTATAAAAAAGGGCGAACCCTAAAAAACTCCATCAAAATGGACCGCACTTTGCTTAGTAATCGACCTCGTGTCTGTCGCAGAATGTCCGTCTTCCCGAc                                                                                                                                                                                          >  1:700076/1‑201 (MQ=255)                                                                                                      aTGTCCAGGTCGAATAAGCGTTGCCCATCGACTAACGCAACGCGCAACTCTTCTTTTTGAGTCGCATTGATTAACATTCTTTTCATTCGTTTTCTCTTTTATAAAAAAGGGCGAACCCTAAAAAACTCCATCAAAATGGACCGCACTTTGCTTAGTAATCGACCTCGTGTCTGTCGCAGAATGTCCGTCTTCCCGACCGTCATCTGCTGGGTGCATTGACTACCTATTAAAATCAATCGAATGTCGAAAtt                                                                                                                                    <  1:95063/251‑1 (MQ=255)                                                                                                        gTCCAGGTCGAATAAGCGTTGCCCATCGACTAACGCAACGCGCAACTCTTCTTTTTGAGTCGCATTGATTAACATTCTTTTCATTCGTTTTCTCTTTTATAAAAAAGGGCGAACCCTAAAAAACTCCATCAAAATGGACCGCACTTTGCTTAGTAATCGACCTCGTGTCTGTCGCAGAATGTCCGTCTTCCCGACCGTCATCTGCTGGGTGCATTGACTACCTATTAAAATCAATCGAATGTCGAAAtttt                                                                                                                                  >  1:419605/1‑251 (MQ=255)                                                                                                        gTCCAGGTCGAATAAGCGTTGCCCATCGACTAACGCAACGCGCAACTCTTCTTTTTGAGTCGCATTGATTAACATTCTTTTCATTCGTTTTCTCTTTTATAAAAAAGGGCGAACCCTAAAAAACTCCATCAAAATGGACCGCACTTTGCTTAGTAATCGACCTCGTGTCTGTCGCAGAATGTCCGTCTTCCCGACCGTCATCTGCTGGGTGCATTGACTACCTATTAAAATCAATCGAATGTCGAAAtttt                                                                                                                                  >  1:342988/1‑251 (MQ=255)                                                                                                                        cGTTGCCCATCGACTAACGCAACGCGCAACTCTTCTTTTTGAGTCGCATTGATTAACATTCTTTTCATTCGTTTTCTCTTTTATAAAAAAGGGCGAACCCTAAAAAACTCCATCAAAATGGACCGCACTTTGCTTAGTAATCGACCTCGTGTCTGTCGCAGAATGTCCGTCTTCCCGACCGTCATCTGCTGGGTGCATTGACTACCTATTAAAATCAATCGAATGTCGAAATTTTAATAAAACAAAAcc                                                                                                                    <  1:200698/249‑1 (MQ=255)                                                                                                                          ttGCCCATCGACTAACGCAACGCGCAACTCTTCTTTTTGAGTCGCATTGATTAACATTCTTTTCATTCGTTTTCTCTTTTATAAAAAAGGGCGAACCCTAAAAAACTCCATCAAAATGGACCGCACTTTGCTTAGTAATCGACCTCGTGTCTGTCGCAGAATGTCCGTCTTCCCGACCGTCATCTGCTGGGTGCATTGACTACCTATTAAAATCAATCGAATGTCGAAATTTTAATAAAACAAAACCTGtt                                                                                                                <  1:634329/251‑1 (MQ=255)                                                                                                                             cccATCGACTAACGCAACGCGCAACTCTTCTTTTTGAGTCGCATTGATTAACATTCTTTTCATTCGTTTTCTCTTTTATAAAAAAGGGCGAACCCTAAAAAACTCCATCAAAATGGACCGCACTTTGCTTAGTAATCGACCTCGTGTCTGTCGCAGAATGTCCGTCTTCCCGACCGTCATCTGCTGGGTGCATTGACTACCTATTAAAATCAATCGAATGTCGAAATTTTAATAAAACAAAACCTGTTAGa                                                                                                             >  1:50032/1‑251 (MQ=255)                                                                                                                                   gACTAACGCAACGCGCAACTCTTCTTTTTGAGTCGCATTGATTAACATTCTTTTCATTCGTTTTCTCTTTTATAAAAAAGGGCGAACCCTAAAAAACTCCATCAAAATGGACCGCACTTTGCTTAGTAATCGACCTCGTGTCTGTCGCAGAATGTCCGTCTTCCCGACCGTCATCTGCTGGGTGCATCGACTACCTATTAAAATCAATCGAATGTCGAAATTTTAATAAAACAAAACCTGTTAGAAAAAAt                                                                                                       >  1:635035/1‑251 (MQ=255)                                                                                                                                       aacgcaacgcGCAACTCTTCTTTTTGAGTCGCATTGATTAACATTCTTTTCATTCGTTTTCTCTTTTATAAAAAAGGGCGAACCCTAAAAAACTCCATCAAAATGGACCGCACTTTGCTTAGTAATCGACCTCGTGTCTGTCGCAGAATGTCCGTCTTCCCGACCGTCATCTGCTGGGTGCATTGACTACCTATTAAAATCAATCGAATGTCGAAATTTTAATAAAACAAAACCTGTTAGAAAAAATGGcc                                                                                                   <  1:481421/251‑1 (MQ=255)                                                                                                                                             acgcGCAACTCTTCTTTTTGAGTCGCATTGATTAACATTCTTTTCATTCGTTTTCTCTTTTATAAAAAAGGGCGAACCCTAAAAAACTCCATCAAAATGGACCGCACTTTGCTTAGTAATCGACCTCGTGTCTGTCGCAGAATGTCCGTCTTCCCGACCGTCATCTGCTGGGTGCATTGACTACCTATTaaaa                                                                                                                                                       <  1:536668/193‑1 (MQ=255)                                                                                                                                              cgcgCAACTCTTCTTTTTGAGTCGCATTGATTAACATTCTTTTCATTCGTTTTCTCTTTTATAAAAAAGGGCGAACCCTAAAAAACTCCATCAAAATGGACCGCACTTTGCTTAGTAATCGACCTCGTGTCTGTCGCAGAATGTCCGTCTTCCCGACCGTCATCTGCTGGGTGCATTGACTACCTATTAAAATCAATCGAATGTCGAAATTTTAATAAAACAAAACCTGTTAGAAAAAATGGCCTGCAAGc                                                                                            <  1:356925/251‑1 (MQ=255)                                                                                                                                               gcgcAACTCTTCTTTTTGAGTCGCATTGATTAACATTCTTTTCATTCGTTTTCTCTTTTATAAAAAAGGGCGAACCCTAAAAAACTCCATCAAAATGGACCGCACTTTGCTTAGTAATCGACCTCGTGTCTGTCGCAGAATGTCCGTCTTCCCGACCGTCATCTGCTGGGTGCATTGACTACCTATTa                                                                                                                                                          >  1:782799/1‑188 (MQ=255)                                                                                                                                                    aCTCTTCTTTTTGAGTCGCATTGATTAACATTCTTTTCATTCGTTTTCTCTTTTATAAAAAAGGGCGAACCCTAAAAAACTCCATCAAAATGGACCGCACTTTGCTTAGTAATCGACCTCGTGTCTGTCGCAGAATGTCCGTCTTCCCGACCGTCATCTGCTGGGTGCATTGACTACCTATTAAAATCAATCGAATGTCGAAATTTTAATAAAACAAAACCTGTTAGAAAAAATGGCCTGCAAGCCGACGt                                                                                      >  1:550052/1‑251 (MQ=255)                                                                                                                                                              ttGAGTCGCATTGATTAACATTCTTTTCATTCGTTTTCTCTTTTATAAAAAAGGGCGAACCCTAAAAAACTCCATCAAAATGGACCGCACTTTGCTTAGTAATCGACCTCGTGTCTGTCGCAGAATGTCCGTCTTCCCGACCGTCATCTGCTGGGTGCATTGACTACCTATTAAAATCAATCGAATGTCGAAATTTTAATAAAACAAAGCCTGTTAGAAAAAATGGCCTGCAAGCCGACGTTCATTTTGAc                                                                            <  1:486549/251‑1 (MQ=255)                                                                                                                                                                     gCATTGATTAACATTCTTTTCATTCGTTTTCTCTTTTATAAAAAAGGGCGAACCCTAAAAAACTCCATCAAAATGGACCGCACTTTGCTTAGTAATCGACCTCGTGTCTGTCGCAGAATGTCCGTCTTCCCGACCGTCATCTGCTGGGTGCATTGACTACCTATTAAAATCAATCGAATGTCGAAATTTTAATAAAACAAAACCTGTTAGAAAAAATGGCCTGCAAGCCGACGTTCAttt                                                                                <  1:326195/240‑1 (MQ=255)                                                                                                                                                                        ttGATTAACATTCTTTTCATTCGTTTTCTCTTTTATAAAAAAGGGCGAACCCTAAAAAACTCCATCAAAATGGACCGCACTTTGCTTAGTAATCGACCTCGTGTCTGTCGCAGAATGTCCGTCTTCCCGACCGTCATCTGCTGGGTGCATTGACTACCTATTAAAATCAATCGAATGTCGAAATTTTAATAAAACAAAACCTGTTAGAAAAAATGGCCTGCAAGCCGACGTTCATTTTGACGCTAAAAAAt                                                                  >  1:794482/1‑251 (MQ=255)                                                                                                                                                                          gATTAACATTCTTTTCATTCGTTTTCTCTTTTATAAAAAAGGGCGAACCCTAAAAAACTCCATCAAAATGGACCGCACTTTGCTTAGTAATCGACCTCGTGTCTGTCGCAGAATGTCCGTCTTCCCGACCGTCATCTGCTGGGTGCATTGACTACCTATTAAAATCAATCGAATGTCGAAATTTTAATAAAACAAAACCTGTTAGAAAAAATGGCCTGCAAGCCGACGTTCATTTTGACGCTAAAAAATCa                                                                >  1:293119/1‑251 (MQ=255)                                                                                                                                                                          gATTAACATTCTTTTCATTCGTTTTCTCTTTTATAAAAAAGGGCGAACCCTAAAAAACTCCATCAAAATGGACCGCACTTTGCTTAGTAATCGACCTCGTGTCTGTCGCAGAATGTCCGTCTTCCCGACCGTCATCTGCTGGGTGCATTGACTACCTATTAAAATCAATCGAATGTCGAAATTTTAATAAAACAAAACCTGTTAGAAAAAATGGCCTGCAAGCCGACGTTCATTTTGACGCTAAAAAATCa                                                                >  1:474758/1‑251 (MQ=255)                                                                                                                                                                               aCATTCTTTTCATTCGTTTTCTCTTTTATAAAAAAGGGCGAACCCTAAAAAACTCCATCAAAATGGACCGCACTTTGCTTAGTAATCGACCTCGTGTCTGTCGCAGAATGTCCGTCTTCCCGACCGTCATCTGCTGGGTGCATTGACTACCTATTAAAATCAATCGAATGTCGAAATTTTAATAAAACAAAACCTGTTAGaaaa                                                                                                          >  1:800334/1‑204 (MQ=255)                                                                                                                                                                               aCATTCTTTTCATTCGTTTTCTCTTTTATAAAAAAGGGCGAACCCTAAAAAACTCCATCAAAATGGACCGCACTTTGCTTAGTAATCGACCTCGTGTCTGTCGCAGAATGTCCGTCTTCCCGACCGTCATCTGCTGGGTGCATTGACTACCTATTAAAATCAATCGAATGTCGAAATTTTAATAAAACAAAACCTGTTAGAAAAAATGGCCTGCAAGCCGACGTTCATTTTGACGCTAAAAAATCATTGa                                                            >  1:503466/1‑250 (MQ=255)                                                                                                                                                                                     ttttCATTCGTTTTCTCTTTTATAAAAAAGGGCGAACCCTAAAAAACTCCATCAAAATGGACCGCACGTTGCTTAGTAATCGACCTCGTGTCTGTCGCAGAATGTCCGTCTTCCCGACCGTCATCTGCTGGGTGCATTGACTACCTATTAAAATCAATCGAATGTCGAAATTTTAATAAAACAAAACCTGTTAGAAAAAATGGCCTGCAAGCCGACGTTCATTTTGACGCTAAAAAATCATTGACCTATCa                                                     <  1:617227/251‑1 (MQ=255)                                                                                                                                                                                      tttCATTCGTTTTCTCTTTTATAAAAAAGGGCGAACCCTAAAAAACTCCATCAAAATGGACCGCACTTTGCTTAGTAATCGACCTCGTGTCTGTCGCAGAATGTCCGTCTTCCCGACCGTCATCTGCTGGGTGCATTGACTACCTATTAAAATCAATCGAATGTCGAAATTTTAATAAAACAAAACCTGTTAGAAAAAATGGCCTGCAAGCCGACGTTCATTTTGACGCTAAAAAATCATTGACCTATCaa                                                    <  1:46684/251‑1 (MQ=255)                                                                                                                                                                                             cGTTTTCTCTTTTATAAAAAAGGGCGAACCCTAAAAAACTCCATCAAAATGGACCGCACTTTGCTTAGTAATCGACCTCGTGTCTGTCGCAGAATGTCCGTCTTCCCGACCGTCATCTGCTGGGTGCATTGACTACCTATTAAAATCAATCGAATGTCGAAATTTTAATAAAACAAAACCTGTTAGAAAAAATGGCCTGCAAGCCGACGTTCATTTTGACGCTAAAAAATCATTGACCTATCAATGTCTTa                                             >  1:124799/1‑251 (MQ=255)                                                                                                                                                                                                tttCTCTTTTATAAAAAAGGGCGAACCCTAAAAAACTCCATCAAAATGGACCGCACTTTGCTTAGTAATCGACCTCGTGTCTGTCGCAGAATGTCCGTCTTCCCGACCGTCATCTGCTGGGTGCATTGACTACCTATTAAAATCAATCGAATGTCGAAATTTTAATAAAACAAAACCTGTTAGAAAAAATGGCCTGCAAGCCGACGTTCATTTTGACGCTAAAAAATCATTGACCTATCAATGTCTTATGc                                          <  1:800266/251‑1 (MQ=255)                                                                                                                                                                                                     ctTTTATAAAAAAGGGCGAACCCTAAAAAACTCCATCAAAATGGACCGCACTTTGCTTAGTAATCGACCTCGTGTCTGTCGCAGAATGTCCGTCTTCCCGACCGTCATCTGCTGGGTGCATTGACTACCTATTAAAATCAATCGAATGTCGAAATTTTAATAAAACAAAACCTGTTAGAAAAAATGGCCTGCAAGCCGACGTTCATTTTGACGCTAAAAAATCATTGACCTATCAATGTCTTATGCCGAAt                                     <  1:519694/251‑1 (MQ=255)                                                                                                                                                                                                       tttATAAAAAAGGGCGAACCCTAAAAAACTCCATCAAAATGGACCGCACTTTGCTTAGTAATCGACCTCGTGTCTGTCGCAGAATGTCCGTCTTCCCGACCGTCATCTGCTGGGTGCATTGACTACCTATTAAAATCAATCGAATGTCGAAATTTTAATAAAACAAAACCTGTTAGAAAAAATGGCCTGCAAGCCGACGTTCATTTTGACGCTAAAAAATCATTGACCTATCAATGTCTTATGCCGAAtgc                                   <  1:568503/251‑1 (MQ=255)                                                                                                                                                                                                        ttATAAAAAAGGGCGAACCCTAAAAAACTCCATCAAAATGGACCGCACTTTGCTTAGTAATCGACCTCGTGTCTGTCGCAGAATGTCCGTCTTCCCGACCGTCATCTGCTGGGTGCATTGACTACCTATTAAAATCAATCGAATGTCGAAATTTTAATAAAACAAAACCTGTTAGAAAAAATGGCCTGCAAGCCGACGTTCATTTTGACGCTAAAAAATCATTGACCTATCAATGTCTTATGCCg                                        >  1:687952/1‑245 (MQ=255)                                                                                                                                                                                                            aaaaaaGGGCGAACCCTAAAAAACTCCATCAAAATGGACCGCACTTTGCTTAGTAATCGACCTCGTGTCTGTCGCAGAATGTCCGTCTTCCCGACCGTCATCTGCTGGGTGCATTGACTACCTATTAAAATCAATCGAATGTCGAAATTTTAATAAAACAAAACCTGTTAGAAAAAATGGCCTGCAAGCCGACGTTCATTTTGACGCTAAAAAATCATTGACCTATCAATGTCTTATGCCGAATGCTGCAt                              <  1:15282/251‑1 (MQ=255)                                                                                                                                                                                                                  gggCGAACCCTAAAAAACTCCATCAAAATGGACCGCACTTTGCTTAGTAATCGACCTCGTGTCTGTCGCAGAATGTCCGTCTTCCCGACCGTCATCTGCTGGGTGCATTGACTACCTATTAAAATCAATCGAATGTCGAAATTTTAATAAAACAAAACCTGTTAGAAAAAATGGCCTGCAAGCCGACGTTCATTTTGACGCTAAAAAATCATTGAcc                                                          <  1:758394/217‑1 (MQ=255)                                                                                                                                                                                                                  gggCGAACCCTAAAAAACTCCATCAAAATGGACCGCACTTTGCTTAGTAATCGACCTCGTGTCTGTCGCAGAATGTCCGTCTTCCCGACCGTCATCTGCTGGGTGCATTGACTACCTATTAAAATCAATCGAATGTCGAAATTTTAATAAAACAAAACCTGTTAGAAAAAATGGCCTGCAAGCCGACGTTCATTTTGACGCTAAAAAATCATTGACCTATCAATGTCTTATGCCGAATGCTGCATTatat                         >  1:890355/1‑250 (MQ=255)                                                                                                                                                                                                                         cccTAAAAAACTCCATCAAAATGGACCGCACTTTGCTTAGTAATCGACCTCGTGTCTGTCGCAGAATGTCCGTCTTCCCGACCGTCATCTGCTGGGTGCATTGACTACCTATTAAAATCAATCGAATGTCGAAATTTTAATAAAACAAAACCTGTTAGAAAAAATGGCCTGCAAGCCGACGTTCATTTTGACGCTAAAAAATCATTGACCTATCAATGTCTTATGCCGAAtgc                                   <  1:613239/233‑1 (MQ=255)                                                                                                                                                                                                                         cccTAAAAAACTCCATCAAAATGGACCGCACTTTGCTTAGTAATCGACCTCGTGTCTGTCGCAGAATGTCCGTCTTCCCGACCGTCATCTGCTGGGTGCATTGACTACCTATTAAAATCAATCGAATGTCGAAATTTTAATAAAACAAAACCTGTTAGAAAAAATGGCCTGCAAGCCGACGTTCATTTTGACGCTAAAAAATCATTGACCTATCAATGTCTTATGCCGAATGCTGCAt                              <  1:342733/238‑1 (MQ=255)                                                                                                                                                                                                                         cccTAAAAAACTCCATCAAAATGGACCGCACTTTGCTTAGTAATCGACCTCGTGTCTGTCGCAGAATGTCCGTCTTCCCGACCGTCATCTGCTGGGTGCATTGACTACCTATTAAAATCAATCGAATGTCGAAATTTTAATAAAACAAAACCTGTTAGAAAAAATGGCCTGCAAGCCGACGTTCATTTTGACGCTAAAAAATCATTGACCTATCAATGTCTTATGCCGAATGCTGCATTatat                         <  1:384063/243‑1 (MQ=255)                                                                                                                                                                                                                         cccTAAAAAACTCCATCAAAATGGACCGCACTTTGCTTAGTAATCGACCTCGTGTCTGTCGCAGAATGTCCGTCTTCCCGACCGTCATCTGCTGGGTGCATTGACTACCTATTAAAATCAATCGAATGTCGAAATTTTAATAAAACAAAACCTGTTAGAAAAAATGGCCTGCAAGCCGACGTTCATTTTGACGCTAAAAAATCATTGACCTATCAATGTCTTATGCCGAATGCTGCATTATATcc                       <  1:716340/245‑1 (MQ=255)                                                                                                                                                                                                                          ccTAAAAAACTCCATCAAAATGGACCGCACTTTGCTTAGTAATCGACCTCGTGTCTGTCGCAGAATGTCCGTCTTCCCGACCGTCATCTGCTGGGTGCATTGACTACCTATTAAAATCAATCGAATGTCGAAATTTTAATAAAACAAAACCTGTTAGAAAAAATGGCCTGCAAGCCGACGTTCATTTTGACGCTAAAAAATCATTGACCTATCAATGTCTTATGCCGAATGCTGCATTATATCCCGTCAAt                >  1:167851/1‑251 (MQ=255)                                                                                                                                                                                                                                        tCAAAATGGACCGCACTTTGCTTAGTAATCGACCTCGTGTCTGTCGCAGAATGTCCGTCTTCCCGACCGTCATCTGCTGGGTGCATTGACTACCTATTAAAATCAATCGAATGTCGAAATTTTAATAAAACAAAACCTGTTAGAAAAAATGGCCTGCAAGCCGACGTTCATTTTGACGCTAAAAAATCATTGACCTATCAATGTCTTATGCCGAATGCTGCATTATATCCCGTCAATGATAACGtatttat  <  1:114832/251‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                              CTGCACCGTAATCCACAAAAGCGGCTTCCAAACTCGGCTCAACCCGAGTAATTTTTCCCTTGTAAATATTGGCTTTTTTCTGTTCATGCCCCAGACTCTCAATGTCCAGGTCGAATAAGCGTTGCCCATCGACTAACGCAACGCGCAACTCTTCTTTTTGAGTCGCATTGATTAACATTCTTTTCATTCGTTTTCTCTTTTATAAAAAAGGGCGAACCCTAAAAAACTCCATCAAAATGGACCGTACTTTGCTTAGTAATCGACCTCGTGTCTGTCGCAGAATGTCCGTCTTCCCGACCGTCATCTGCTGGGTGCATTGACTACCTATTAAAATCAATCGAATGTCGAAATTTTAATAAAACAAAACCTGTTAGAAAAAATGGCCTGCAAGCCGACGTTCATTTTGACGCTAAAAAATCATTGACCTATCAATGTCTTATGCCGAATGCTGCATTATATCCCGTCAATGATAACGTATTTAT  >  1/2091865‑2092346

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑