Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	280,785	G→A	D80D (GAC→GAT)	unknown ←	LSU ribosomal protein L15p (L27Ae)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	280,785	0	G	A	100.0%	211.1 / NA	62	D80D (GAC→GAT)	unknown	LSU ribosomal protein L15p (L27Ae)
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (35/27);  total (35/27)

TTGTACCACCTTGAGTACTTCTTGTTTGATAGCCTGGGTTGTTTAGCCATTAATTATTCCTCGATTGAACCACCAGCAGCTTCAATAGCTGCTTTGGCGCCTTTAGTCACACGCAACCCACGAATAACTAATTTATTTTTAACTTCACCCGCTAAAATTACTTTAGCAAACTGAATATCCTTAGTTAAAATATTGGCAGCTTTTAATGTTTCTAAAGTCACAATACCATTTTCAATTTTATTCAGGTCGTCTAGACGGACTTCTGCTGTTACTGCAGATTTCATAGAAGTAAAACCAAATTTAGGTAAACGACGATATAAAGGCATTTGACCACCTTCGAACCCACGACGAACACCGCCACCAGTACGAGATTTTTGACCTTTATGACCACGTCCACCAGTTTTACCTAAACCTGAACCAATACCACGACCAAGAAGTTTAGCACTATGCTTAGCACCTTCAGCCGGAGATAGAGTATTTAAGCG  >  1/280537‑281021                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                             ttGTACCACCTTGAGTACTTCTTGTTTGATAGCCT‑GGTTGTTTAGCCATTAATTATTCCTCGATTGAACCACCAGCAGCTTCAATAGCTGCTTTGGCGCCTTTAGTCACACGCAACCCACGAATAACTAATTTATTTTTAACTTCACCCGCTAAAATTACTTTAGCAAACTGAATATCCTTAGTTAAAATATTGGCAGCTTTTAATGTTTCTAAAGTCACAATACCATTTTCAATTTTATTCAGGTCATCt                                                                                                                                                                                                                                           <  1:109170/251‑1 (MQ=255)         ccTTGAGTACTTCTTGTTTGATAGCCT‑GGTTGTTTAGCCATTAATTATTCCTCGATTGAACCACCAGCAGCTTCAATAGCTGCTTTGGCGCCTTTAGTCACACGCAACCCACGAATAACTAATTTATTTTTAACTTCACCCGCTAAAATTACTTTAGCAAACTGAATATCCTTAGTTAAAATATTGGCAGCTTTTAATGTTTCTAAAGTCACAATACCATTTTCAATTTTATTCAGGTCATCTAGACGGa                                                                                                                                                                                                                                    >  1:519632/1‑250 (MQ=255)                tACTTCTTGTTTGATAGCCT‑GGTTGTTTAGCCATTAATTATTCCTCGATTGAACCACCAGCAGCTTCAATAGCTGCTTTGGCGCCTTTAGTCACACGCAACCCACGAATAACTAATTTATTTTTAACTTCACCCGCTAAAATTACTTTAGCAAACTGAATATCCTTAGTTAAAATATTGGCAGCTTTTAATGTTTCTAAAGTCACAATACCATTTTCAATTTTATTCAGGTCATCTAGACGGACTTctgc                                                                                                                                                                                                                             <  1:848937/250‑1 (MQ=255)                 aCTTCTTGTTTGATAGCCT‑GGTTGTTTAGCCATTAATTATTCCTCGATTGAACCACCAGCAGCTTCAATAGCTGCTTTGGCGCCTTTAGTCACACGCAACCCACGAATAACTAATTTATTTTTAACTTCACCCGCTAAAATTACTTTAGCAAACTGAATATCCTTAGTTAAAATATTGGCAGCTTTTAATGTTTCTAAAGTCACAATACCATTTTCAATTTTATTCAGGTCATCTAGACGGACTTctgctg                                                                                                                                                                                                                           <  1:610241/251‑1 (MQ=255)                 aCTTCTTGTTTGATAGCCT‑GGTTGTTTAGCCATTAATTATTCCTCGATTGAACCACCAGCAGCTTCAATAGCTGCTTTGGCGCCTTTAGTCACACGCAACCCACGAATAACTAATTTATTTTTAACTTCACCCGCTAAAATTACTTTAGCAAACTGAATATCCTTAGTTAAAATATTGGCAGCTTTTAATGTTTCTAAAGTCACAATACCATTTTCAATTTTATTCAGGTCATCTAGACGGACTTctgctg                                                                                                                                                                                                                           <  1:372943/251‑1 (MQ=255)                      ttGTTTGATAGCCT‑GGTTGTTTAGCCATTAATTATTCCTCGATTGAACCACCAGCAGCTTCAATAGCTGCTTTGGCGCCTTTAGTCACACGCAACCCACGAATAACTAATTTATTTTTAACTTCACCCGCTAAAATTACTTTAGCAAACTGAATATCCTTAGTTAAAATATTGGCAGCTTTTAATGTTTCTAAAGTCACAATACCATTTTCAATTTTATTCAGGTCATCTAGACGGACTTCTGCTGTTa                                                                                                                                                                                                                        >  1:498563/1‑249 (MQ=255)                                    tggTTGTTTAGCCATTAATTATTCCTCGATTGAACCACCAGCAGCTTCAATAGCTGCTTTGGCGCCTTTAGTCACACGCAACCCACGAATAACTAATTTATTTTTAACTTCACCCGCTAAAATTACTTTAGCAAACTGAATATCCTTAGTTAAAATATTGGCAGCTTTTAATGTTTCTAAAGTCACAATACCATTTTCAATTTTATTCAGGTCATCTAGACGGACTTctgc                                                                                                                                                                                                                             <  1:277609/230‑1 (MQ=255)                                                      ttattCCTCGATTGAACCACCAGCAGCTTCAATAGCTGCTTTGGCGCCTTTAGTCACACGCAACCCACGAATAACTAATTTATTTTTAACTTCACCCGCTAAAATTACTTTAGCAAACTGAATATCCTTAGTTAAAATATTGGCAGCTTTTAATGTTTCTAAAGTCACAATACCATTTTCAATTTTATTCAGGTCATCTAGACGGACTTCTGCTGTTACTGCAGATTTCATAGAAGTAAAACCAAATTTAg                                                                                                                                                                                       >  1:41910/1‑251 (MQ=255)                                                              cGATTGAACCACCAGCAGCTTCAATAGCTGCTTTGGCGCCTTTAGTCACACGCAACCCACGAATAACTAATTTATTTTTAACTTCACCCGCTAAAATTACTTTGGCAAACTGAATACCCTTAGTTAAAATATTGGCAGCTTTTAATGTTTCTAAAGTCACAATACCATTTTCAATTTTATTCAGGTCATCTAGACGGACTTCTGCTGTTACTGCAGATTTCATAGAAGTAAAACCAAATTTAGGTAAacga                                                                                                                                                                               >  1:694182/1‑251 (MQ=255)                                                              cGATTGAACCACCAGCAGCTTCAATAGCTGCTTTGGCGCCTTTAGTCACACGCAACCCACGAATAACTAATTTATTTTTAACTTCACCCGCTAAAATTACTTTAGCAAACTGAATATCCTTAGTTAAAATATTGGCAGCTTTTAATGTTTCTAAAGTCACAATACCATTTTCAATTTTATTCAGGTCATCTAGACGGACTTCTGCTGTTACTGCAGATTTCATAGAAGTAAAACCAAATTTAGGTAAacga                                                                                                                                                                               <  1:157287/251‑1 (MQ=255)                                                                    aaCCACCAGCAGCTTCAATAGCTGCTTTGGCGCCTTTAGTCACACGCAACCCACGAATAACTAATTTATTTTTAACTTCACCCGCTAAAATTACTTTAGCAAACTGAATATCCTTAGTTAAAATATTGGCAGCTTTTAATGTTTCTAAAGTCACAATACCATTTTCAATTTTATTCAGGTCATCTAGACGGACTTCTGCTGTTACTGCAGATTTCATAGAAGTAAAACCAAATTTAGGTAAACGACGatat                                                                                                                                                                         >  1:891639/1‑251 (MQ=255)                                                                         ccaGCAGCTTCAATAGCTGCTTTGGCGCCTTTAGTCACACGCAACCCACGAATAACTAATTTATTTTTAACTTCACCCGCTAAAATTACTTTAGCAAACTGAATATCCTTAGTTAAAATATTGGCAGCTTTTAATGTTTCTAAAGTCACAATACCATTTTCAATTTTATTCAGGTCATCTAGACGGACTTCTGCTGTTACTGCAGATTTCATAGAAGTAAAACCAAATTTAGGTAAACGACGATATAAAgg                                                                                                                                                                    <  1:524707/251‑1 (MQ=255)                                                                               gcTTCAATAGCTGCTTTGGCGCCTTTAGTCACACGCAACCCACGAATAACTAATTTATTTTTAACTTCACCCGCTAAAATTACTTTAGCAAACTGAATATCCTTAGTTAAAATATTGGCAGCTTTTAATGTTTCTAAAGTCACAATACCATTTTCAATTTTATTCAGGTCATCTAGACGGACTTCTGCTGTTACTGCAGATTTCATAGAAGTAAAACCAAATTTAGGTAAacgac                                                                                                                                                                              >  1:630370/1‑235 (MQ=255)                                                                                            ctTTGGCGCCTTTAGTCACACGCAACCCACGAATAACTAATTTATTTTTAACTTCACCCGCTAAAATTACTTTAGCAAACTGAATATCCTTAGTTAAAATATTGGCAGCTTTTAATGTTTCTAAAGTCACAATACCATTTTCAATTTTATTCAGGTCATCTAGACGGACTTCTGCTGTTACTGCAGATTTCATAGAAGTAAAACCAAATTTAGGTAAACGACGATATAAAGGCATTTGACCACCTTCGAAc                                                                                                                                                 <  1:223631/251‑1 (MQ=255)                                                                                                    ccTTTAGTCACACGCAACCCACGAATAACTAATTTATTTTTAACTTCACCCGCTAAAATTACTTTAGCAAACTGAATATCCTTAGTTGAAATATTGGCAGCTTTTAATGTTTCTAAAGTCACAATACCATTTTCAATTTTATTCAGGTCATCTAGACGGACTTCTGCTGTTACTGCAGATTTCATAGAAGTAAAACCAAATTTAGGTAAACGACGATATAAAGGCATTTGACCACCTTCGAACCCacgacg                                                                                                                                         >  1:624985/1‑251 (MQ=255)                                                                                                     cTTTAGTCACACGCAACCCACGAATAACTAATTTATTTTTAACTTCACCCGCTAAAATTACTTTAGCAAACTGAATATCCTTAGTTAAAATATTGGCAGCTTTTAATGTTTCTAAAGTCACAATACCATTTTCAATTTTATTCAGGTCATCTAGACGGACTTCTGCTGTTACTGCAGATTTCATAGAAGTAAAACCAAATTTAGGTAAACGACGATATAAAGGCATTTGACCACCTTCGAACCCAcgacga                                                                                                                                        >  1:472560/1‑251 (MQ=255)                                                                                                       ttAGTCACACGCAACCCACGAATAACTAATTTATTTTTAACTTCACCCGCTAAAATTACTTTAGCAAACTGAATATCCTTAGTTAAAATATTGGCAGCTTTTAATGTTTCTAAAGTCACAATACCATTTTCAATTTTATTCAGGTCATCTAGACGGACTTCTGCTGTTACTGCAGATTTCATAGAAGTAAAACCAAATTTAGGTAAACGACGATATAAAGGCATTTGACCACCTTCGAACCCACGACGAac                                                                                                                                      <  1:508538/251‑1 (MQ=255)                                                                                                          gTCACACGCAACCCACGAATAACTAATTTATTTTTAACTTCACCCGCTAAAATTACTTTAGCAAACTGAATATCCTTAGTTAAAATATTGGCAGCTTTTAATGTTTCTAAAGTCACAATACCATTTTCAATTTTATTCAGGTCATCt                                                                                                                                                                                                                                           <  1:451974/147‑1 (MQ=255)                                                                                                                cGCAACCCACGAATAACTAATTTATTTTTAACTTCACCCGCTAAAATTACTTTAGCAAACTGAATATCCTTAGTTAAAATATTGGCAGCTTTTAATGTTTCTAAAGTCACAATACCATTTTCAATTTTATTCAGGTCATCTAGACGGACTTCTGCTGTTACTGCAGATTTCATAGAAGTAAAACCAAATTTAGGTAAACGACGATATAAAGGCATTTGACCACCTTCGAACCCACGACGAACACCGccacc                                                                                                                             <  1:468417/251‑1 (MQ=255)                                                                                                                  cAACCCACGAATAACTAATTTATTTTTAACTTCACCCGCTAAAATTACTTTAGCAAACTGAATATCCTTAGTTAAAATATTGGCAGCTTTTAATGTTTCTAAAGTCACAATACCATTTTCAATTTTATTCAGGTCATCTAGACGGACTTCTGCTGTTACTGCAGATTTCAt                                                                                                                                                                                                           >  1:275674/1‑171 (MQ=255)                                                                                                                    aCCCACGAATAACTAATTTATTTTTAACTTCACCCGCTAAAATTACTTTAGCAAACTGAATATCCTTAGTTAAAATATTGGCAGCTTTTAATGTTTCTAAAGTCACAATACCATTTTCAATTTTATTCAGGTCATCTAGACGGACTTCTGCTGTTACTGCAGATTTCATAGAAGTAAAACCAAATTTAGGTAAACGACGATATAAAgg                                                                                                                                                                    <  1:267150/208‑1 (MQ=255)                                                                                                                    aCCCACGAATAACTAATTTATTTTTAACTTCACCCGCTAAAATTACTTTAGCAAACTGAATATCCTTAGTTAAAATATTGGCAGCTTTTAATGTTTCTAAAGTCACAATACCATTTTCAATTTTATTCAGGTCATCTAGACGGACTTCTGCTGTTACTGCAGATTTCATAGAAGTAAAACCAAATTTAGGTAAACGACGATATAAAGGCATTTGACCACCTTCGAAc                                                                                                                                                 >  1:283202/1‑227 (MQ=255)                                                                                                                    aCCCACGAATAACTAATTTATTTTTAACTTCACCCGCTAAAATTACTTTAGCAAACTGAATATCCTTAGTTAAAATATTGGCAGCTTTTAATGTTTCTAAAGTCACAATACCATTTTCAATTTTATTCAGGTCATCTAGACGGACTTCTGCTGTTACTGCAGATTTCATAGAAGTAAAACCAAATTTAGGTAAACGACGATATAAAGGCATTTGACCACCTTCGAACCCACGACGAACACCGCCACCAGTa                                                                                                                         >  1:704932/1‑251 (MQ=255)                                                                                                                    aCCCACGAATAACTAATTTATTTTTAACTTCACCCGCTAAAATTACTTTAGCAAACTGAATATCCTTAGTTAAAATATTGGCAGCTTTTAATGTTTCTAAAGTCACAATACCATTTTCAATTTTATTCAGGTCATCTAGACGGACTTCTGCTGTTACTGCAGATTTCATAGAAGTAAAACCAAATTTAGGTAAACGACGATATAAAGGCATTTGACCACCTTCGAACCCACGACGAACACCGCCACCAGTa                                                                                                                         >  1:370198/1‑251 (MQ=255)                                                                                                                                cTAATTTATTTTTAACTTCACCCGCTAAAATTACTTTAGCAAACTGAATATCCTTAGTTAAAATATTGGCAGCTTTTAATGTTTCTAAAGTCACAATACCATTTTCAATTTTATTCAGGTCATCTAGACGGACTTCTGCTGTTACTGCAGATTTCATAGAAGTAAAACCAAATTTAGGTAAACGACGATATAAAGGCATTTGACCACCTTCGAACCCACGACGAACACCGCCACCAGTa                                                                                                                         <  1:843360/239‑1 (MQ=255)                                                                                                                                       atttTTAACTTCACCCGCTAAAATTACTTTAGCAAACTGAATATCCTTAGTTAAAATATTGGCAGCTTTTAATGTTTCTAAAGTCACAATACCATTTTCAATTTTATTCAGGTCATCTAGACGGACTTCTGCTGTTACTGCAGATTTCATAGAAGTAAAACCAAATTTAGGTAAACGACGATATAAAGGCATTTGac                                                                                                                                                            >  1:149725/1‑197 (MQ=255)                                                                                                                                        tttttAACTTCACCCGCTAAAATTACTTTAGCAAACTGAATATCCTTAGTTAAAATATTGGCAGCTTTTAATGTTTCTAAAGTCACAATACCATTTTCAATTTTATTCAGGTCATCTAGACGGACTTCTGCTGTTACTGCAGATTTCATAGAAGTAAAACCAAATTTAGGTAAACGACGATATAAAGGCATTTGACCACCTTCGAACCCACGACGAACACCGCCACCAGTACGAGATTTTTGACCTTTATg                                                                                                     >  1:291081/1‑251 (MQ=255)                                                                                                                                          tttAACTTCACCCGCTAAAATTACTTTAGCAAACTGAATATCCTTAGTTAAAATATTGGCAGCTTTTAATGTTTCTAAAGTTACAATACCATTTTCAATTTTATTCAGGTCATCTAGACGGACTTCTGCTGTTACTGCAGATTTCATAGAAGTAAAACCAAATTTAGGTAAACGACGATATAAAGGCATTTGACCACCTTCGAACCCACGACGAACACCGCCACCAGTACGAGATTTTTGACCTTTATGAc                                                                                                   <  1:445665/251‑1 (MQ=255)                                                                                                                                             aaCTTCACCCGCTAAAATTACTTTAGCAAACTGAATATCCTTAGTTAAAATATTGGCAGCTTTTAATGTTTCTAAAGTCACAATACCATTTTCAATTTTATTCAGGTCATCTAGACGGACTTCTGCTGTTACTGCAGATTTCATAGAAGTAAAACCAAATTTAGGTAAACGACGATATAAAGGCATTTGACCACCTTCGAACCCACGACGAACACCGCCACCAGTACGAGATTTTTGACCTTTATGACCa                                                                                                 >  1:528994/1‑250 (MQ=255)                                                                                                                                                        cTAAAATTACTTTAGCAAACTGAATATCCTTAGTTAAAATATTGGCAGCTTTTAATGTTTCTAAAGTCACAATACCATTTTCAATTTTATTCAGGTCATCTAGACGGACTTCTGCTGTTACTGCAGATTTCATAGAAGTAAAACCAAATTTAGGTAAACGACGATATAAAGGCATTTGACCACCTTCGAACCCACGACGAACACCGCCACCAGTACGAGATTTTTGACCTTTATGACCACGTCCACCAGtt                                                                                     >  1:578762/1‑251 (MQ=255)                                                                                                                                                                   ttAGCAAACTGAATATCCTTAGTTAAAATATTGGCAGCTTTTAATGTTTCTAAAGTCACAATACCATTTTCAATTTTATTCAGGTCATCTAGACGGACTTCTGCTGTTACTGCAGATTTCATAGAAGTAAAACCAAATTTAGGTAAACGACGATATAAAGGCATTTGACCACCTTCGAACCCACGACGAACACCGCCACCAGTACGAGATTTTTGACCTTTATGACCACGTCCACCAGTTTTACCTAAAcc                                                                          <  1:807418/251‑1 (MQ=255)                                                                                                                                                                         aaCTGAATATCCTTAGTTAAAATATTGGCAGCTTTTAATGTTTCTAAAGTCACAATACCATTTTCAATTTTATTCAGGTCATCTAGACGGACTTCTGCTGTTACTGCAGATTTCATAGAAGTAAAACCAAATTTAGGTAAACGACGATATAAAGGCATTTGACCACCTTCGAACCCACGACGAACACCGCCACCAGTACGAGATTTTTGACCTTTATGACCACGTCCACCAGTTTTACCTAAACCTGAAcc                                                                    <  1:163631/251‑1 (MQ=255)                                                                                                                                                                         aaCTAAATATCCTTAGTTAAAATATTGGCAGCTTTTAATGTTTCTAAAGTCACAATACCATTTTCAATTTTATTCAGGTCATCTAGACGGACTTCTGCTGTTACTGCAGATTTCATAGAAGTAAAACCAAATTTAGGTAAACGACGATATAAAGGCATTTGACCACCTTCGAACCCACGACGAACACCGCCACCAg                                                                                                                           >  1:731260/1‑196 (MQ=255)                                                                                                                                                                           cTGAATATCCTTAGTTAAAATATTGGCAGCTTTTAATGTTTCTAAAGTCACAATACCATTTTCAATTTTATTCAGGTCATCTAGACGGACTTCTGCTGTTACTGCAGATTTCATAGAAGTAAAACCAAATTTAGGTAAACGACGATATAAAGGCATTTGACCACCTTCGAACCCACGACGAACACCGCCACCAGTACGAGATTTTTGACCTTTATGACCACGTCCACCAGTTTTACCTAAACCTGAACCaa                                                                  >  1:107989/1‑251 (MQ=255)                                                                                                                                                                             gAATATCCTTAGTTAAAATATTGGCAGCTTTTAATGTTTCTAAAGTCACAATACCATTTTCAATTTTATTCAGGTCATCTAGACGGACTTCTGCTGTTACTGCAGATTTCATAGAAGTAAAACCAAATTTAGGTAAACGACGATATAAAGGCATTTGACCACCTTCGAACCCACGACGAACACCGCCACCAGTACGAGATTTTTGACCTTTATGACCACGTCCACCAGTTTTACCTAAACCTGAAcc                                                                    >  1:698411/1‑247 (MQ=255)                                                                                                                                                                                  tCCTTAGTTAAAATATTGGCAGCTTTTAATGTTTCTAAAGTCACAATACCATTTTCAATTTTATTCAGGTCATCTAGACGGACTTCTGCTGTTACTGCAGATTTCATAGAAGTAAAACCAAATTTAGGTAAACGACGATATAAAGGCATTTGACCACCTTCGAACCCACGACGAACACCGCCACCAGTACGAGATTTTTGACCTTTATGACCACGTCCACCAGTTTTACCTAAACCTGAACCAATACCACg                                                           >  1:458699/1‑251 (MQ=255)                                                                                                                                                                                       aGTTAAAATATTGGCAGCTTTTAATGTTTCTAAAGTCACAATACCATTTTCAATTTTATTCAGGTCATCTAGACGGACTTCTGCTGTTa                                                                                                                                                                                                                        >  1:188070/1‑89 (MQ=255)                                                                                                                                                                                        gTTAAAATATTGGCAGCTTTTAATGTTTCTAAAGTCACAATACCATTTTCAATTTTATTCAGGTCATCTAGACGGACTTCTGCTGTTACTGCAGATTTCATAGAAGTAAAACCAAATTTAGGTAAACGACGATATAAAGGCATTTGaccac                                                                                                                                                         <  1:239719/151‑1 (MQ=255)                                                                                                                                                                                        gTTAAAATATTGGCAGCTTTTAATGTTTCTAAAGTCACAATACCATTTTCAATTTTATTCAGGTCATCTAGACGGACTTCTGCTGTTACTGCAGATTTCATAGAAGTAAAACCAAATTTAGGTAAACGACGATATAAAGGCATTTGACCACCTTCGAAc                                                                                                                                                 <  1:198393/159‑1 (MQ=255)                                                                                                                                                                                        gTTAAAATATTGGCAGCTTTTAATGTTTCTAAAGTCACAATACCATTTTCAATTTTATTCAGGTCATCTAGACGGACTTCTGCTGTTACTGCAGATTTCATAGAAGTAAAACCAAATTTAGGTAAACGACGATATAAAGGCATTTGACCACCTTCGAACCCACGACGAACACCGCCACCAGTACGAGATTTTTGACCTTTATGACCACGTCCACCAGTTTTACCTAAACCTGAACCAATACCACGACCaag                                                     >  1:305641/1‑251 (MQ=255)                                                                                                                                                                                           aaaaTATTGGCAGCTTTTAATGTTTCTAAAGTCACAATACCATTTTCAATTTTATTCAGGTCATCTAGACGGACTTCTGCTGTTACTGCAGATTTCATAGAAGTAAAACCAAATTTAGGTAAACGACGATATAAAGGCATTTGACCACCTTCGAACCCACGACGAACACCGCCACCAGTACGAGATTTTTGACCTTTATGACCACGTCCACCAGTTTTACCTAAACCTGAACCAATACCACGACCaagaag                                                  <  1:768516/251‑1 (MQ=255)                                                                                                                                                                                           aaaaTATTGGCAGCTTTTAATGTTTCTAAAGTCACAATACCATTTTCAATTTTATTCAGGTCATCTAGACGGACTTCTGCTGTTACTGCAGATTTCATAGAAGTAAAACCAAATTTAGGTAAACGACGATATAAAGGCATTTGACCACCTTCGAACCCACGACGAACACCGCCACCAGTACGAGATTTTTGACCTTTATGACCACGTCCACCAGTTTTACCTAAACCTGAACCAATACCACGACCaagaag                                                  <  1:601329/251‑1 (MQ=255)                                                                                                                                                                                            aaaTATTGGCAGCTTTTAATGTTTCTAAAGTCACAATACCATTTTCAATTTTATTCAGGTCATCTAGACGGACTTCTGCTGTTACTGCAGATTTCATAGAAGTAAAACCAAATTTAGGTAAACGACGATATAAAGGCATTTGACCACCTTCGAACCCACGACGAACACCGCCACCAGTACGAGATTTTTGACCTTTATGACCACGTCCACCAGTTTTACCTAAACCTGAAc                                                                     >  1:611247/1‑231 (MQ=255)                                                                                                                                                                                                  tGGCAGCTTTTAATGTTTCTAAAGTCACAATACCATTTTCAATTTTATTCAGGTCATCTAGACGGACTTCTGCTGTTACTGCAGATTTCATAGAAGTAAAACCAAATTTAGGTAAACGACGATATAAAGGCATTTGACCACCTTCGAACCCACGACGAACACCGCCACCAGTACGAGATTTTTGACCTTTATGACCACGTCCACCAGTTTTACCTAAACCTGAACCAATACCACGACCAAGAAGTTTAGCa                                           >  1:789756/1‑251 (MQ=255)                                                                                                                                                                                                    gCAGCTTTTAATGTTTCTAAAGTCACAATACCATTTTCAATTTTATTCAGGTCATCTAGACGGACTTCTGCTGTTACTGCAGATTTCATAGAAGTAAAACCAAATTTAGGTAAACGACGATATAAAGGCATTTGACCACCTTCGAACCCACGACGAACACCGCCACCAGTACGAGATTTTTGACCTTTATGACCACGTCCACCAGTTTTACCTAAACCTGAACCAATACCACGACCAAGAAGTTTAGCAc                                          <  1:329878/250‑1 (MQ=255)                                                                                                                                                                                                       gCTTTTAATGTTTCTAAAGTCACAATACCATTTTCAATTTTATTCAGGTCATCTAGACGGACTTCTGCTGTTACTGCAGATTTCATAGAAGTAAAACCAAATTTAGGTAAACGACGATATAAAGGCATTTGACCACCTTCGAACCCACGACGAACACCGCCACCAGTACAAGATTTTTGACCTTTATGACCACGTCCACCAGTTTTACCTAAACCTGAACCAATACCACGACCAAGAAGTTTAGCACTATg                                      >  1:426964/1‑251 (MQ=255)                                                                                                                                                                                                        cTTTTAATGTTTCTAAAGTCACAATACCATTTTCAATTTTATTCAGGTCATCTAGACGGACTTCTGCTGTTACTGCAGATTTCATAGAAGTAAAACCAAATTTAGGTAAACGACGATATAAAgg                                                                                                                                                                    >  1:822553/1‑124 (MQ=255)                                                                                                                                                                                                        cTTTTAATGTTTCTAAAGTCACAATACCATTTTCAATTTTATTCAGGTCATCTAGACGGACTTCTGCTGTTACTGCAGATTTCATAGAAGTAAAACCAAATTTAGGTAAACGACGATATAAAGGCATTTGACCACCTTCGAACCCACGACGAACACCGCCACCAGTACGAGATTTTTGACCTTTATGACCACGTCCACCAGTTTTACCTAAACCTGAACCAATAc                                                               <  1:871384/225‑1 (MQ=255)                                                                                                                                                                                                        cTTTTAATGTTTCTAAAGTCACAATACCATTTTCAATTTTATTCAGGTCATCTAGACGGACTTCTGCTGTTACTGCAGATTTCATAGAAGTAAAACCAAATTTAGGTAAACGACGATATAAAGGCATTTGACCACCTTCGAACCCACGACGAACACCGCCACCAGTACGAGATTTTTGACCTTTATGACCACGTCCACCAGTTTTACCTAAACCTGAACCAATACCACGACCAAGAAGTTTAGCAc                                          >  1:564267/1‑246 (MQ=255)                                                                                                                                                                                                             aaTGTTTCTAAAGTCACAATACCATTTTCAATTTTATTCAGGTCATCTAGACGGACTTCTGCTGTTACTGCAGATTTCATAGAAGTAAAACCAAATTTAGGTAAACGACGATATAAAGGCATTTGACCACCTTCGAACCCACGACGAACACCGCCACCAGTACGAGATTTTTGACCTTTATGACCACGTCCACCAGTTTTACCTAAACCTGAACCAATACCACGACCAAGAAGTTTAGCACTATGCTTAGc                                <  1:322596/251‑1 (MQ=255)                                                                                                                                                                                                               tGTTTCTAAAGTCACAATACCATTTTCAATTTTATTCAGGTCATCTAGACGGACTTCTGCTGTTACTGCAGATTTCATAGAAGTAAAACCAAATTTAGGTAAACGACGATATAAAGGCATTTGACCACCTTCGAACCCACGACGAACACCGCCACCAGTACGAGATTTTTGACCTTTATGACCACGTCCACCAGTTTTACCTAAACCTGAACCAATACCACGACCAAGAAGTTTAGCACTATGCtt                                   >  1:338856/1‑246 (MQ=255)                                                                                                                                                                                                                         gTCACAATACCATTTTCAATTTTATTCAGGTCATCTAGACGGACTTCTGCTGTTACTGCAGATTTCATAGAAGTAAAACCAAATTTAGGTAAACGACGATATAAAGGCATTTGACCACCTTCGAACCCACGACGAACACCGCCACCAGTACGAGATTTTTGACCTTTATGACCACGTCCACCAGTTTTACCTAAACCTGAACCAATACCACGACCAAGAAGTTTAGCAc                                          >  1:331307/1‑229 (MQ=255)                                                                                                                                                                                                                                 aCCATTTTCAATTTTATTCAGGTCATCTAGACGGACTTCTGCTGTTACTGCAGATTTCATAGAAGTAAAACCAAATTTAGGTAAACGACGATATAAAGGCATTTGACCACCTTCGAACCCACGACGAACACCGCCACCAGTACGAGATTTTTGACCTTTATGACCACGTCCACCAGTTTTACCTAAACCTGAACCAATACCACGACCAAGAAGTTTAGCACTATGCTTAGCACCTTCAGCCGGAGATagag            <  1:17423/251‑1 (MQ=255)                                                                                                                                                                                                                                 aCCATTTTCAATTTTATTCAGGTCATCTAGACGGACTTCTGCTGTTACTGCAGATTTCATAGAAGTAAAACCAAATTTAGGTAAACGACGATATAAAGGCATTTGACCACCTTCGAACCCACGACGAACACCGCCACCAGTACGAGATTTTTGACCTTTATGACCACGTCCACCAGTTTTACCTAAACCTGAACCAATACCACGACCAAGAAGTTTAGCACTATGCTTAGCACCTTCAGCCGGAGATagag            >  1:552955/1‑251 (MQ=255)                                                                                                                                                                                                                                 aCCATTTTCAATTTTATTCAGGTCATCTAGACGGACTTCTGCTGTTACTGCAGATTTCATAGAAGTAAAACCAAATTTAGGTAAACGACGATATAAAGGCATTTGACCACCTTCGAACCCACGACGAACACCGCCACCAGTACGAGATTTTTGACCTTTATGACCACGTCCACCAGTTTTACCTAAACCTGAACCAATACCACGACCAAGAAGTTTAGCACTATGCTTAGCACCTTCAGCCGGAGATagag            <  1:901119/251‑1 (MQ=255)                                                                                                                                                                                                                                       ttCAATTTTATTCAGGTCATCTAGACGGACTTCTGCTGTTACTGCAGATTTCATAGAAGTAAAACCAAATTTAGGTAAACGACGATATAAAGGCATTTGACCACCTTCGAACCCACGACGAACACCGCCACCAGTACGAGATTTTTGACCTTTATGACCACGTCCACCAGTTTTACCTAAACCTGAACCAATACCACGACCAAGAAGTTTAGCACTATGCTTAGCACCTTCAGCCGGAGATAGAGTATTTa      <  1:582335/251‑1 (MQ=255)                                                                                                                                                                                                                                         cAATTTTATTCAGGTCATCTAGACGGACTTCTGCTGTTACTGCAGATTTCATAGAAGTAAAACCAAATTTAGGTAAACGACGATATAAAGGCATTTGACCACCTTCGAACCCACGACGAACACCGCCACCAGTACGAGATTTTTGACCTTTATGACCACGTCCACCAGTTTTACCTAAACCTGAACCAATACCACGACCAAGAAGTTTAGCACTATGCTTAGCACCTTCAGCCGGAGATAGAGTATTTAAg    <  1:628098/251‑1 (MQ=255)                                                                                                                                                                                                                                           aTTTTATTCAGGTCATCTAGACGGACTTCTGCTGTTACTGCAGATTTCATAGAAGTAAAACCAAATTTAGGTAAACGACGATATAAAGGCATTTGACCACCTTCGAACCCACGACGAACACCGCCACCAGTACg                                                                                                                       >  1:559590/1‑134 (MQ=255)                                                                                                                                                                                                                                           aTTTTATTCAGGTCATCTAGACGGACTTCTGCTGTTACTGCAGATTTCATAGAAGTAAAACCAAATTTAGGTAAACGACGATATAAAGGCATTTGACCACCTTCGAACCCACGACGAACACCGCCACCAGTACGAGATTTTTGACCTTTATGACCACGTCCACCAGTTTTACCTAAACCTGAACCAATACCACGACCAAGAAGTTTAGCACTATGCTTAGCACCTTCAGCCGGAGATAGAGTATTTAAgcg  >  1:412839/1‑251 (MQ=255)                                                                                                                                                                                                                                           aTTTTATTCAGGTCATCTAGACGGACTTCTGCTGTTACTGCAGATTTCATAGAAGTAAAACCAAATTTAGGTAAACGACGATATAAAGGCATTTGACCACCTTCGAACCCACGACGAACACCGCCACCAGTACGAGATTTTTGACCTTTATGACCACGTCCACCAGTTTTACCTAAACCTGAACCAATACCACGACCAAGAAGTTTAGCACTATGCTTAGCACCTTCAGCCGGAGATAGAGTATTTAAg    >  1:758578/1‑249 (MQ=255)                                                                                                                                                                                                                                              ttATTCAGGTCATCTAGACGGACTTCTGCTGTTACTGCAGATTTCATAGAAGTAAAACCAAATTTAGGTAAACGACGATATAAAGGCATTTGACCACCTTCGAACCCACGACGAACACCGCCACCAGTACGAGATTTTTGACCTTTATGACCACGTCCACCAGTTTTACCTAAACCTGAACCAATACCACGACCAAGAAGTTTAGCACTATGCTTAGCACCTTCAGCCGGAGATAGAGTATTTAAgcg  >  1:433193/1‑248 (MQ=255)                                                                                                                                                                                                                                                aTTCAGGTCATCTAGACGGACTTCTGCTGTTACTGCAGATTTCATAGAAGTAAAACCAAATTTAGGTAAACGACGATATAAAGGCATTTGACCACCTTCGAACCCACGACGAACACCGCCACCAGTAc                                                                                                                        >  1:130780/1‑128 (MQ=255)                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                             TTGTACCACCTTGAGTACTTCTTGTTTGATAGCCTGGGTTGTTTAGCCATTAATTATTCCTCGATTGAACCACCAGCAGCTTCAATAGCTGCTTTGGCGCCTTTAGTCACACGCAACCCACGAATAACTAATTTATTTTTAACTTCACCCGCTAAAATTACTTTAGCAAACTGAATATCCTTAGTTAAAATATTGGCAGCTTTTAATGTTTCTAAAGTCACAATACCATTTTCAATTTTATTCAGGTCGTCTAGACGGACTTCTGCTGTTACTGCAGATTTCATAGAAGTAAAACCAAATTTAGGTAAACGACGATATAAAGGCATTTGACCACCTTCGAACCCACGACGAACACCGCCACCAGTACGAGATTTTTGACCTTTATGACCACGTCCACCAGTTTTACCTAAACCTGAACCAATACCACGACCAAGAAGTTTAGCACTATGCTTAGCACCTTCAGCCGGAGATAGAGTATTTAAGCG  >  1/280537‑281021

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑