Predicted mutation
evidence seq id position mutation annotation gene description
RA 1 1,596,143 G→A A337V (GCC→GTC)  unknown ← Phosphoglycerate transport system sensor protein PgtB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*11,596,1430GA100.0% 77.2 / NA 24A337V (GCC→GTC) unknownPhosphoglycerate transport system sensor protein PgtB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (11/13);  total (11/13)

AAAATTTGATTTCTTCTTTCTAATTCTTCATTTTTTGTGTTAACTTCAAATAAAAATAATCGCAATAATTTTGCGATACGCCCTAATTCATCATTACCATAAACTGAAATTTTAACGGAAGATTCGCCGTTAATAAGCCGTTCAACAGATTGATTAAGAGATTGAAAACGCCCTATTAATCTCCGTCGAATATAATAAAAATTAATTGCTGCAACAAAAAGAAAAACAAATAAGGCGGCAGACAGAATAATCAAGCCATTTATTCTGGTGCTTTTATTTATTAAATAATGAAACAATTCTAATTGTTTTTGATTATCACCAATCTGACTGTTAATTTTCCTTCTTAATTTAGAAATGACCAATTCACTTTCATTCATTAATTGCCGTTGGGAAATATCCAGTTGTTTTCTTTCAACTAATAAATGAGGTAACGATTGTTCATTTTCTCCGATAGCCAGCAAGGACT  >  1/1595909‑1596374
                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                       
aaaaTTTGATTTCTTCTTTCTAATTCTTCATTTTTTGTGTTAACTTCAAATAAAAATAATCGCAATAATTTTGCGATACGCCCTAATTCATCATTACCATAAACTGAAATTTTAACGGAAGATTCGCCGTTAATAAGCCGTTCAACAGATTGATTAAGAGATTGAAAACGCCCTATTAATCTCCGTCGAATATAATAAAAATTAATTGCTGCAACAAAAAGAAAAACAAATAAGACGGCAGACAGaataat                                                                                                                                                                                                                         <  1:673203/251‑1 (MQ=255)
            cttcttTCTAATTCTTCATTTTTTGTGTTAACTTCAAATAAAAATAATCGCAATAATTTTGCGATACGCCCTAATTCATCATTACCATAAACTGAAATTTTAACGGAAGATTCGCCGTTAATAAGCCGTTCAACAGATTGATTAAGAGATTGAAAACGCCCTATTAATCTCCGTCGAATATAATAAAAATTAATTGCTGCAACAAAAAGAAAAACAAATAAGACGGCAGACAGAATAATCa                                                                                                                                                                                                                       <  1:183682/241‑1 (MQ=255)
                                           cTTCAAATAAAAATAATCGCAATAATTTTGCGATACGCCCTAATTCATCATTACCATAAACTGAAATTTTAACGGAAGATTCGCCGTTAATAAGCCGTTCAACAGATTGATTAAGAGATTGAAAACGCCCTATTAATCTCCGTCGAATATAATAAAAATTAATTGCTGCAACAAAAAGAAAAACAAATAAGACGGCAGACAGAATAATCAAGcc                                                                                                                                                                                                                   >  1:619722/1‑214 (MQ=255)
                                                aataaaaataaTCGCAATAATTTTGCGATACGCCCTAATTCATCATTACCATAAACTGAAATTTTAACGGAAGATTCGCCGTTAATAAGCCGTTCAACAGATTGATTAAGAGATTGAAAACGCCCTATTAATCTCCGTCGAATATAATAAAAATTAATTGCTGCAACAAAAAGAAAAACAAATAAGACGGCAGACAGAATAATCAAGCCATTTATTCTGGTGCTTTTATTTATTAAATAATGAAACAATTc                                                                                                                                                                         <  1:728638/251‑1 (MQ=255)
                                                              cAATAATTTTGCGATACGCCCTAATTCATCATTACCATAAACTGAAATTTTAACGGAAGATTCGCCGTTAATAAGCCGTTCAACAGATTGATTAAGAGATTGAAAACGCCCTATTAATCTCCGTCGAATATAATAAAAATTAATTGCTGCAACAAAAAGAAAAACAAATAAGACGGCAGACAGAATAATCAAGCCATTTATTCTGGTGCTTTTATTTATTAAATAATGAAACAATTCTAATTGTTTTTGAt                                                                                                                                                           >  1:842519/1‑251 (MQ=255)
                                                                         cGATACGCCCTAATTCATCATTACCATAAACTGAAATTTTAACGGAAGATTCGCCGTTAATAAGCCGTTCAACAGATTGATTAAGAGATTGAAAACGCCCTATTAATCTCCGTCGAATATAATAAAAATTAATTGCTGCAACAAAAAGAAAAACAAATAAGACGGCAGACAGAATAATCAAGCCATTTATTCTGGTGCTTTTATTTATTAAATAATGAAACAATTCTAATTGTTTTTGATTATCACCAATc                                                                                                                                                <  1:770403/251‑1 (MQ=255)
                                                                                    aaTTCATCATTACCATAAACTGAAATTTTAACGGAAGATTCGCCGTTAATAAGCCGTTCAACAGATTGATTAAGAGATTGAAAACGCCCTATTAATCTCCGTCGAATATAATAAAAATTAATTGCTGCAACAAAAAGAAAAACAAATAAGACGGCAGACAGAATAATCAAGCCATTTATTc                                                                                                                                                                                                           >  1:24145/1‑181 (MQ=255)
                                                                                        catcatTACCATAAACTGAAATTTTAACGGAAGATTCGCCGTTAATAAGCCGTTCAACAGATTGATTAAGAGATTGAAAACGCCCTATTAATCTCCGTCGAATATAATAAAAATTAATTGCTGCAACAAAAAGAAAAACAAATAAGACGGCAGACAGAATAATCa                                                                                                                                                                                                                       <  1:173783/165‑1 (MQ=255)
                                                                                          tcatTACCATAAACTGAAATTTTAACGGAAGATTCGCCGTTAATAAGCCGTTCAACAGATTGATTAAGAGATTGAAAACGCCCTATTAATCTCCGTCGAATATAATAAAAATTAATTGCTGCAACAAAAAGAAAAACAAATAAgacggc                                                                                                                                                                                                                                     <  1:447404/149‑1 (MQ=255)
                                                                                               aCCATAAACTGAAATTTTAACGGAAGATTCGCCGTTAATAAGCCGTTCAACAGATTGATTAAGAGATTGAAAACGCCCTATTAATCTCCGTCGAATATAATAAAAATTAATTGCTGCAACAAAAAGAAAAACAAATAAGACGGCAGACAGAATAATCAAGCCATTTATTCTGGTGCTTTTATTTATTAaat                                                                                                                                                                                      >  1:362661/1‑191 (MQ=255)
                                                                                                                         aTTCGCCGTTAATAAGCCGTTCAACAGATTGATTAAGAGATTGAAAACGCCCTATTAATCTCCGTCGAATATAATAAAAATTAATTGCTGCAACAAAAAGAAAAACAAATAAGACGGCAGACAGAATAATCAAGCCATTTATTCTGGTGCTTTTATTTATTAAATAATGAAACAATTCTAATTGTTTTTGATTATCACCAATCTGACTGTTAATTTTCCTTCTTAATTTAGAAATGACCAATTCACttt                                                                                                  <  1:614116/249‑1 (MQ=255)
                                                                                                                                gTTAATAAGCCGTTCAACAGATTGATTAAGAGATTGAAAACGCCCTATTAATCTCCGTCGAATATAATAAAAATTAATTGCTGCAACAAAAAGAAAAACAAATAAGACGGCAGACAGAATAATCAAGCCATTTATTCTGGTGCTTTTATTTATTAAATAATGAAACAATTCTAATTGTTTTTGATTATCAc                                                                                                                                                     >  1:84164/1‑191 (MQ=255)
                                                                                                                                gTTAATAAGCCGTTCAACAGATTGATTAAGAGATTGAAAACGCCCTATTAATCTCCGTCGAATATAATAAAAATTAATTGCTGCAACAAAAAGAAAAACAAATAAGACGGCAGACAGAATAATCAAGCCATTTATTCTGGTGCTTTTATTTATTAAATAATGAAACAATTCTAATTGTTTTTGATTATCACCAATCTGACTGTTAATTTTc                                                                                                                                 >  1:165528/1‑211 (MQ=255)
                                                                                                                                           gTTCAACAGATTGATTAAGAGATTGAAAACGCCCTATTAATCTCCGTCGAATATAATAAAAATTAATTGCTGCAACAAAAAGAAAAACAAATAAGACGGCAGACAGAATAATCAAGCCATTTATTCTGGTGCTTTTATTTATTAAATAATGAAACAATTCTAATTGTTTTTGATTATCACCAATc                                                                                                                                                >  1:697663/1‑185 (MQ=255)
                                                                                                                                                    attgattAAGAGATTGAAAACGCCCTATTAATCTCCGTCGAATATAATAAAAATTAATTGCTGCAACAAAAAGAAAAACAAATAAGACGGCAGACAGAATAATCAAGCCATTTATTCTGGTGCTTTTATTTATTAAATAATGAAACAATTCTAATTGTTTTTGATTATCAc                                                                                                                                                     <  1:604941/171‑1 (MQ=255)
                                                                                                                                                               gaTTGAAAACGCCCTATTAATCTCCGTCGAATATAATAAAAATTAATTGCTGCAACAAAAAGAAAAACAAATAAGACGGCAGACAGAATAATCAAGCCATTTATTCTGGTGCTTTTATTTATTAAATAATGAAACAATTCTAATTGTTTTTGATTATCACCAATCTGACTGTTAATTTTCCTTCTTAATTTAGAAATGACCAATTCACTTTCATTCATTAATTGCCGTTGGGAAATATcc                                                                     <  1:544855/240‑1 (MQ=255)
                                                                                                                                                                            cTATTAATCTCCGTCGAATATAATAAAAATTAATTGCTGCAACAAAAAGAAAAACAAATAAGACGGCAGACAGAATAATCAAGCCATTTATTCTGGTGCTTTTATTTATTAAATAATGAAACAATTCTAATTGTTTTTGATTATCACCAATCTGACTGTTAATTTTCCTTCTTAATTTAGAAATGACCAATTCACTTTCATTCATTAATTGCCGTTGGGAAATATCCAGTTGTTTTCTTTCAACTAATaaa                                             >  1:382187/1‑251 (MQ=255)
                                                                                                                                                                            cTATTAATCTCCGTCGAATATAATAAAAATTAATTGCTGCAACAAAAAGAAAAACAAATAAGACGGCAGACAGAATAATCAAGCCATTTATTCTGGTGC‑TTTATTTATTAAATAATGAAACAATTCTAATTGTTTTTGATTATCAcc                                                                                                                                                    >  1:16602/1‑147 (MQ=255)
                                                                                                                                                                              aTTAATCTCCGTCGAATATAATAAAAATTAATTGCTGCAACAAAAAGAAAAACAAATAAGACGGCAGACAGAATAATCAAGCCATTTATTCTGGTGCTTTTATTTATTAAATAATGAAACAATTCTAATTGTTTTTGATTATCACCAATCTGACTGTTAATTTTCCTTCTTAATTTAGAAATGACCAATTCACTTTCATTCATTAATTGCCGTTGGGAAATATCCAgt                                                                  >  1:652064/1‑228 (MQ=255)
                                                                                                                                                                                      ccGTCGAATATAATAAAAATTAATTGCTGCAACAAAAAGAAAAACAAATAAGACGGCAGACAGAATAATCAAGCCATTTATTCTGGTGCTTTTATTTATTAAATAATGAAACAATTCTAATTGTTTTTGATTATCACCAATCTGACTGTTAATTTTCCTTCTTAATTTAGAAATGACCAATTCACTTTCATTCATTa                                                                                         >  1:96663/1‑197 (MQ=255)
                                                                                                                                                                                                                ctgcAACAAAAAGAAAAACAAATAAGACGGCAGACAGAATAATCAAGCCATTTATTCTGGTGCTTTTATTTATTAAATAATGAAACAATTCTAATTGTTTTTGATTATCACCAATc                                                                                                                                                <  1:382984/116‑1 (MQ=255)
                                                                                                                                                                                                                      caaAAAGAAAAACAAATAAGACGGCAGACAGAATAATCAAGCCATTTATTCTGGTGCTTTTATTTATTAAATAATGAAACAATTCTAATTGTTTTTGATTATCACCAATCTGACTGTTAATTTTCCTTCTTAATTTAGAAATGACCAATTCACTTTCATTCATTAATTGCCGTTGGGAAATATCCAGTTGTTTTCTTTCAACTAATAAATGAGGTAACGATTGTTCATTTTCTCCGATAGCCAGCAAGGAc   <  1:46492/251‑1 (MQ=255)
                                                                                                                                                                                                                       aaaaaGAAAAACAAATAAGACGGCAGACAGAATAATCAAGCCATTTATTCTGGTGCTTTTATTTATTAAATAATGAAACAATTCTAATTGTTTTTGATTATCACCAATCTGACTGTTAATTTTCCTTCTTAATTTAGAAATGACCAATTCACTTTCATTCATTAATTGCCGTTGGGAAATATCCAGTTGTTTTCTTTCAACTAATAAATGAGGTAACGATTGTTCATTTTCTCCGATAGCCAGCAAGGACt  <  1:286855/251‑1 (MQ=255)
                                                                                                                                                                                                                                  cAAATAAGACGGCAGACAGAATAATCAAGCCATTTATTCTGGTGCTTTTATTTATTAAATAATGAAACAATTCTAATTGTTTTTGATTATCACCAATCTGACTGTTAATTTTCCTTCTTAATTTAGAAATGAcc                                                                                                            <  1:232439/134‑1 (MQ=255)
                                                                                                                                                                                                                                  cAAATAAGACGGCAGACAGAATAATCAAGCCATTTATTCTGGTGCTTTTATTTATTAAATAATGAAACAATTCTAATTGTTTTTGATTATCACCAATCTGACTGTTAATTTTCCTTCTTAATTTAGAAATGACCAATTCACTTTCATTCATTAATTGCCGTTGGGAAATATCCAGTTGTTTTCTTTCAACTAATAAATGAGGTAACGAt                                 <  1:144709/209‑1 (MQ=255)
                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                       
AAAATTTGATTTCTTCTTTCTAATTCTTCATTTTTTGTGTTAACTTCAAATAAAAATAATCGCAATAATTTTGCGATACGCCCTAATTCATCATTACCATAAACTGAAATTTTAACGGAAGATTCGCCGTTAATAAGCCGTTCAACAGATTGATTAAGAGATTGAAAACGCCCTATTAATCTCCGTCGAATATAATAAAAATTAATTGCTGCAACAAAAAGAAAAACAAATAAGGCGGCAGACAGAATAATCAAGCCATTTATTCTGGTGCTTTTATTTATTAAATAATGAAACAATTCTAATTGTTTTTGATTATCACCAATCTGACTGTTAATTTTCCTTCTTAATTTAGAAATGACCAATTCACTTTCATTCATTAATTGCCGTTGGGAAATATCCAGTTGTTTTCTTTCAACTAATAAATGAGGTAACGATTGTTCATTTTCTCCGATAGCCAGCAAGGACT  >  1/1595909‑1596374

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑