Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,594,935	C→T	V12M (GTG→ATG)	unknown ←	Phosphoglycerate transport system transcriptional regulatory protein PgtA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,594,935	0	C	T	100.0%	117.9 / NA	35	V12M (GTG→ATG)	unknown	Phosphoglycerate transport system transcriptional regulatory protein PgtA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (19/16);  total (19/16)

ATTCGAATTGGCCAGCTTCTGTAATTGTTTTTTTAACTCGTTAATCCAAACACTGCTGCCAATAAAAACGTCATTAATTTTTTCCATTTGCCATTGGCGCTTTTCAATCACCTTTTTCCGTTTTTTTAAAGCGTTATCGACTTGAATAAGTAATTTTTCAGGGGAAACCGGTTTTTCAAAGAAATTTGTCGCCCCTTTCTTGACAGCTTCTACCGCCATGGGAACATCACCATGCCCGGTAATCACAATCACCGGAATTTGGCTATCCATTTCAATCATTCGATCTAATCATCTAATCCTGAAATGCCCAGCAATAATACGTCGCAAATCACAACGCCATACCAATTTTCTGGAATTTGATTAATAATCTCTTTAGGTTCGGCAGTTGCAAATACAGAATAGCCTTCTTGTTGTAATAAATCGGCATACGATTCCAAAATATCAAAATCATCATCAATTAATAAAACATTATTCTGATAATTAAAC  >  1/1594690‑1595175                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                 aTTCGAATTGGCCAGCTTCTGTAATTGTTTTTTTAACTCGTTAATCCAAACACTGCTGCCAATAAAAACGTCATTAATTTTTTCCATTTGCCATTGGCGCTTTTCAATCACCTTTTTCCGTTTTTTTAAAGCGTTATCGACTTGAATAAGTAATTTTTCAGGGGAAACCGGTTTTTCAAAGAAATTTGTCGCCCCTTTCTTGACAGCTTCTACCGCCATGGGAACATCACCATGCCCGGTaatcataatc                                                                                                                                                                                                                                              <  1:667980/250‑1 (MQ=255) aTTCGAATTGGCCAGCTTCTGTAATTGTTTTTTTAACTCGTTAATCCAAACACTGCTGCCAATAAAAACGTCATTAATTTTTTCCATTTGCCATTGGCGCTTTTCAATCACCTTTTTCCGTTTTTTTAAAGCGTTATCGACTTGAATAAGTAATTTTTCAGGGGAAACCGGTTTTTCAAAGAAATTTGTCGCCCCTTTCTTGACAGCTTCTACCGCCATGGGAACATCACCATGCCCGGTaatcataatc                                                                                                                                                                                                                                              <  1:651231/250‑1 (MQ=255)               gCTTCTGTAATTGTTTTTTTAACTCGTTAATCCAAACACTGCTGCCAATAAAAACGTCATTAATTTTTTCCATTTGCCATTGGCGCTTTTCAATCACCTTTTTCCGTTTTTTTAAAGCGTTATCGACTTGAATAAGTAATTTTTCAGGGGAAACCGGTTTTTCAAAGAAATTTGTCGCCCCTTTCTTGACAGCTTCTACCGCCATGGGAACATCACCATGCCCGGTAATCATAATCACCGGAATTTGGCTa                                                                                                                                                                                                                               >  1:383133/1‑251 (MQ=255)                   cTGTAATTGTTTTTTTAACTCGTTAATCCAAACACTGCTGCCAATAAAAACGTCATTAATTTTTTCCATTTGCCATTGGCGCTTTTCAATCACCTTTTTCCGTTTTTTTAAAGCGTTATCGACTTGAATAAGTAATTTTTCAGGGGAAACCGGTTTTTCAAAGAAATTTGTCGCCCCTTTCTTGACAGCTTCTACCGCCATGGGAACATCACCATGCCCGGTAATCATAATCACCGGa                                                                                                                                                                                                                                        >  1:4104/1‑238 (MQ=255)                   cTGTAATTGTTTTTTTAACTCGTTAATCCAAACACTGCTGCCAATAAAAACGTCATTAATTTTTTCCATTTGCCATTGGCGCTTTTCAATCACCTTTTTCCGTTTTTTTAAAGCGTTATCGACTTGAATAAGTAATTTTTCAGGGGAAACCGGTTTTTCAAAGAAATTTGTCGCCCCTTTCTTGACAGCTTCTACCGCCATGGGAACATCACCATGCCCGGTAATCATAATCACCGGAATTTGGCTATCCa                                                                                                                                                                                                                           >  1:652071/1‑251 (MQ=255)                   cTGTAATTGTTTTTTTAACTCGTTAATCCAAACACTGCTGCCAATAAAAACGTCATTAATTTTTTCCATTTGCCATTGGCGCTTTTCAATCACCTTTTTCCGTTTTTTTAAAGCGTTATCGACTTGAATAAGTAATTTTTCAGGGGAAACCGGTTTTTCAAAGAAATTTGTCGCCCCTTTCTTGACAGCTTCTACCGCCATGGGAACATCACCATGCCCGGTAATCATAATCACCGGAATTTGGCTATCCa                                                                                                                                                                                                                           >  1:50713/1‑251 (MQ=255)                   cTGTAATTGTTTTTTTAACTCGTTAATCCAAACACTGCTGCCAATAAAAACGTCATTAATTTTTTCCATTTGCCATTGGCGCTTTTCAATCACCTTTTTCCGTTTTTTTAAAGCGTTATCGACTTGAATAAGTAATTTTTCAGGGGAAACCGGTTTTTCAAAGAAATTTGTCGCCCCTTTCTTGACAGCTTCTACCGCCATGGGAACATCACCATGCCCGGTAATCATAATCACCGGAATTTGGCTATCCa                                                                                                                                                                                                                           >  1:42416/1‑251 (MQ=255)                      tAATTGTTTTTTTAACTCGTTAATCCAAACACTGCTGCCAATAAAAACGTCATTAATTTTTTCCATTTGCCATTGGCGCTTTTCAATCACCTTTTTCCGTTTTTTTAAAGCGTTATCGACTTGAATAAGTAATTTTTCAGGGGAAACCGGTTTTTCAAAGAAATTTGTCGCCCCTTTCTTGACAGCTTCTACCGCCATGGGAACATCACCATGCCCGGTAATCATAATCACCGGAATTTGGCTATcc                                                                                                                                                                                                                            <  1:48377/247‑1 (MQ=255)                           ttttttttAACTCGTTAATCCAAACACTGCTGCCAATAAAAACGTCATTAATTTTTTCCATTTGCCATTGGCGCTTTTCAATCACCTTTTTCCGTTTTTTTAAAGCGTTATCGACTTGAATAAGTAATTTTTCAGGGGAAACCGGTTTTTCAAAGAAATTTGTCGCCCCTTTCTTGACAGCTTCTACCGCCATGGGAACATCACCATGCCCGGTAATCATAATCACCGGAATTTGGCTATCCATTTCAATc                                                                                                                                                                                                                   <  1:189470/250‑1 (MQ=255)                            tttttttAACTCGTTAATCCAAACACTGCTGCCAATAAAAACGTCATTAATTTTTTCCATTTGCCATTGGCGCTTTTCAATCACCTTTTTCCGTTTTTTTAAAGCGTTATCGACTTGAATAAGTAATTTTTCAGGGGAAACCGGTTTTTCAAAGAAATTTGTCGCCCCTTTCTTGACAGCTTCTACCGCCATGGGAACATCACCATGCCCGGTAATCATAATCACCGGAATTTGGCTATCCATTTCAATc                                                                                                                                                                                                                   <  1:670996/250‑1 (MQ=255)                                       cGTTAATCCAAACACTGCTGCCAATAAAAACGTCATTAATTTTTTCCATTTGCCATTGGCGCTTTTCAATCACCTTTTTCCGTTTTTTTAAAGCGTTATCGACTTGAATAAGTAATTTTTCAGGGGAAACCGGTTTTTCAAAGAAATTTGTCGCCCCTTTCTTGACAGCTTCTACCGCCATGGGAACATCACCATGCCCGGTAATCATAATCACCGGAATTTGGCTATCCATTTCAATCATTCGatctaat                                                                                                                                                                                                       >  1:604063/1‑251 (MQ=255)                                       cGTTAATCCAAACACTGCTGCCAATAAAAACGTCATTAATTTTTTCCATTTGCCATTGGCGCTTTTCAATCACCTTTTTCCGTTTTTTTAAAGCGTTATCGACTTGAATAAGTAATTTTTCAGGGGAAACCGGTTTTTCAAAGAAATTTGTCGCCCCTTTCTTGACAGCTTCTACCGCCATGGGAACATCACCATGCCCGGTAATCATAATCACCGGAATTTGGCTATCCATTTCAATCATTCGatctaat                                                                                                                                                                                                       >  1:525333/1‑251 (MQ=255)                                            aTCCAAACACTGCTGCCAATAAAAACGTCATTAATTTTTTCCATTTGCCATTGGCGCTTTTCAATCACCTTCTTCCGTTTTTTTAAAGCGTTATCGACTTGAATAAGTAATTTTTCAGGGGAAACCGGTTTTTCAAAGAAATTTGTCGCCCCTTTCTTGACAGCTTCTACCGCCATGGGAACATCACCATGCCCGGTAATCATAATCACCGGAATTTGGCTATCCATTTCAATCATTCGatctaatcat                                                                                                                                                                                                    >  1:11230/1‑249 (MQ=255)                                             tCCAAACACTGCTGCCAATAAAAACGTCATTAATTTTTTCCATTTGCCATTGGCGCTTTTCAATCACCTTTTTCCGTTTTTTTAAAGCGTTATCGACTTGAATAAGTAATTTTTCAGGGGAAACCGGTTTTTCAAAGAAATTTGTCGCCCCTTTCTTGACAGCTTCTACCGCCATGGGAACATCACCATGCCCGGTAATCATAATCACCGGAATTTGGCTATCCATTTCAATCATTCGatctaatcatcta                                                                                                                                                                                                 >  1:863951/1‑251 (MQ=255)                                             tCCAAACACTGCTGCCAATAAAAACGTCATTAATTTTTTCCATTTGCCATTGGCGCTTTTCAATCACCTTTTTCCGTTTTTTTAAAGCGTTATCGACTTGAATAAGTAATTTTTCAGGGGAAACCGGTTTTTCAAAGAAATTTGTCGCCCCTTTCTTGACAGCTTCTACCGCCATGGGAACATCACCATGCCCGGTAATCATAATCACCGGAATTTGGCTATCCATTTCAATCATTCGatctaatcatcta                                                                                                                                                                                                 >  1:246073/1‑251 (MQ=255)                                                      tgctgcCAATAAAAACGTCATTAATTTTTTCCATTTGCCATTGGCGCTTTTCAATCACCTTTTTCCGTTTTTTTAAAGCGTTATCGACTTGAATAAGTAATTTTTCAGGGGAAACCGGTTTTTCAAAGAAATTTGTCGCCCCTTTCTTGACAGCTTCTACCGCCATGGGAACATCACCATGCCCGGTAATCATAATCACCGGAATTTGGCTATCCATTTCAATCATTCGATCTAATCATCTAATCCTGaaa                                                                                                                                                                                        <  1:635549/251‑1 (MQ=255)                                                                aaaaaCGTCATTAATTTTTTCCATTTGCCATTGGCGCTTTTCAATCACCTTTTTCCGTTTTTTTAAAGCGTTATCGACTTGAATAAGTAATTTTTCAGGGGAAACCGGTTTTTCAAAGAAATTTGTCGCCCCTTTCTTGACAGCTTCTACCGCCATGGGAACATCACCATGCCCGGTAATCATAATCACCGGAATTTGGCTATCCATTTCAATCATTCGATCTAATCATCTAATCCTGAAATGCCCAGCaa                                                                                                                                                                              <  1:690893/251‑1 (MQ=255)                                                                       tCATTAATTTTTTCCATTTGCCATTGGCGCTTTTCAATCACCTTTTTCCGTTTTTTTAAAGCGTTATCGACTTGAATAAGTAATTTTTCAGGGGAAACCGGTTTTTCAAAGAAATTTGTCGCCCCTTTCTTGACAGCTTCTACCGCCATGGGAACATCACCATGCCCGGTAATCATAATCACCGGAATTTGGCTATCCATTTCAATCATTCGATCTAATCATCTAATCCTGAAATGCCCAGCAATAATAc                                                                                                                                                                        <  1:267349/250‑1 (MQ=255)                                                                       tCATTAATTTTTTCCATTTGCCATTGGCGCTTTTCAATCACCTTTTTCCGTTTTTTTAAAGCGTTATCGACTTGAATAAGTAATTTTTCAGGGGAAACCGGTTTTTCAAAGAAATTTGTCGCCCCTTTCTTGACAGCTTCTACCGCCATGGGAACATCACCATGCCCGGTAATCATAATCACCGGAATTTGGCTATCCATTTCAATCATTCGATCTAATCATCTAATCCTGAAATGCCCAGCAATAATACg                                                                                                                                                                       >  1:202133/1‑251 (MQ=255)                                                                       tCATTAATTTTTTCCATTTGCCATTGGCGCTTTTCAATCACCTTTTTCCGTTTTTTTAAAGCGTTATCGACTTGAATAAGTAATTTTTCAGGGGAAACCGGTTTTTCAAAAAAATTTGTCGCCCCTTTCTTGACAGCTTCTACCGCCATGGGAACATCACCATGCCCGGTAATCATAATCACCGGAATTTGGCTATCCATTTCAATCATTCGATCTAATCATCTAATCCTGAAATGCCCAGCAATAATAc                                                                                                                                                                        <  1:814775/250‑1 (MQ=255)                                                                        cATTAATTTTTTCCATTTGCCATTGGCGCTTTTCAATCACCTTTTTCCGTTTTTTTAAAGCGTTATCGACTTGAATAAGTAATTTTTCAGGGGAAACCGGTTTTTCAAAGAAATTTGTCGCCCCTTTCTTGACAGCTTCTACCGCCATGGGAACATCACCATGCCCGGTAATCATAATCACCGGAATTTGGCTATCCATTTCAATCATTCGATCTAATCATCTAATCCTGAAATGCCCAGCAATAATACGt                                                                                                                                                                      <  1:703031/251‑1 (MQ=255)                                                                                                        tCAATCACCTTTTTCCGTTTTTTTAAAGCGTTATCGACTTGAATAAGTAATTTTTCAGGGGAAACCGGTTTTTCAAAGAAATTTGTCGCCCCTTTCTTGACAGCTTCTACCGCCATGGGAACATCACCATGCCCGGTAATCATAATCACCGGAATTTGGCTATCCATTTCAATCATTCGATCTAATCATCTAATCCTGAAATGCCCAGCAATAATAc                                                                                                                                                                        <  1:825517/217‑1 (MQ=255)                                                                                                                           tttttAAAGCGTTATCGACTTGAATAAGTAATTTTTCAGGGGAAACCGGTTTTTCAAAGAAATTTGTCGCCCCTTTCTTGACAGCTTCTACCGCCATGGGAACATCACCATGCCCGGTAATCATAATCACCGGAATTTGGCTATCCATTTCAATCATTCGatctaatca                                                                                                                                                                                                     <  1:571426/169‑1 (MQ=255)                                                                                                                             tttAAAGCGTTATCGACTTGAATAAGTAATTTTTCAGGGGAAACCGGTTTTTCAAAGAAATTTGTCGCCCCTTTCTTGACAGCTTCTACCGCCATGGGAACATCACCATGCCCGGTaatcataatc                                                                                                                                                                                                                                              <  1:635772/126‑1 (MQ=255)                                                                                                                                 aaGCGTTATCGACTTGAATAAGTAATTTTTCAGGGGAAACCGGTTTTTCAAAGAAATTTGTCGCCCCTTTCTTGACAGCTTCTACCGCCATGGGAACATCACCATGCCCGGTAATCATAATCACCGGAATTTGGCTATCCATTTCAATCATTCGATCTAATCATCTAATCCTGAAATGCCCAGCAATAATACGTCGCAAATCACAACGCCATACCAATTTTCTGGAATTTGATTAATAATCTCTTTAGGtt                                                                                                             <  1:384619/251‑1 (MQ=255)                                                                                                                                                      gTAATTTTTCAGGGGAAACCGGTTTTTCAAAGAAATTTGTCGCCCCTTTCTTGACAGCTTCTACCGCCATGGGAACATCACCATGCCCGGTAATCATAATCACCGGAATTTGGCTATCCATTTCAATCATTCGATCTAATCATCTAATCCTGAAATGCCCAGCAATAATACGTCGCAAATCACAACGCCATACCAATTTTCTGGAATTTGATTAATAATCTCTTTAGGTTCGGCa                                                                                                        >  1:374872/1‑235 (MQ=255)                                                                                                                                                          tttttCAGGGGAAACCGGTTTTTCAAAGAAATTTGTCGCCCCTTTCTTGACAGCTTCTACCGCCATGGGAACATCACCATGCCCGGTAATCATAATCACCGGAATTTGGCTATCCATTTCAATCATTCGATCTAATCATCTAATCCTGAAATGCCCAGCAATAATACGTCGCAAATCACAACGCCATACCAATTTTCTGGAATTTGATTAATAATCTCTTTAGGTTCGGCAGTTGCAAATACAGAATAGcc                                                                                    <  1:356504/251‑1 (MQ=255)                                                                                                                                                                  gggAAACCGGTTTTTCAAAGAAATTTGTCGCCCCTTTCTTGACAGCTTCTACCGCCATGGGAACATCACCATGCCCGGTAATCATAATCACCGGAATTTGGCTATCCATTTCAATCATTCGATCTAATCATCTAATCCTGAAATGCCCAGCAATAATACGTCGCAAATCACAACGCCATACCAATTTTCTGGAATTTGATTAATAATCTCTTTAGGTTCGGCAGTTGCAAATACAGAATAGCcttctt                                                                               <  1:422623/248‑1 (MQ=255)                                                                                                                                                                  gggAAACCGGTTTTTCAAAGAAATTTGTCGCCCCTTTCTTGACAGCTTCTACCGCCATGGGAACATCACCATGCCCGGTAATCATAATCACCGGAATTTGGCTATCCATTTCAATCATTCGATCTAATCATCTAATCCTGAAATGCCCAGCAATAATACGTCGCAAATCACAACGCCATACCAATTTTCTGGAATTTGATTAATAATCTCTTTAGGTTCGGCAGTTGCAAATACAGAATAGCCTTCttg                                                                              <  1:806412/249‑1 (MQ=255)                                                                                                                                                                                                  ccTTTCTTGACAGCTTCTACCGCCATGGGAACATCACCATGCCCGGTAATCATAATCACCGGAATTTGGCTATCCATTTCAATCATTCGATCTAATCATCTAATCCTGAAATGCCCAGCAATAATACGTCGCAAATCACAACGCCATACCAATTTTCTGGAATTTGATTAATAATCTCTTTAGGTTCGGCAGTTGCAAATACAGAATAGCCTTCTTGTTGTAATAAATCGGCATACGATTCCAAAATATCa                                            >  1:809233/1‑251 (MQ=255)                                                                                                                                                                                                    tttCTTGACAGCTTCTACCGCCATGGGAACATCACCATGCCCGGTAATCATAATCACCGGAATTTGGCTATCCATTTCAATCATTCGATCTAATCATCTAATCCTGAAATGCCCAGCAATAATACGTCGCAAATCACAACGCCATACCAATTTTCTGGAATTTGATTAATAATCTCTTTAGGTTCGGCAGTTGCAAATAc                                                                                             <  1:662463/200‑1 (MQ=255)                                                                                                                                                                                                                        ccATGGGAACATCACCATGCCCGGTAATCATAATCACCGGAATTTGGCTATCCATTTCAATCATTCGATCTAATCATCTAATCCTGAAATGCCCAGCAATAATACGTCGCAAATCACAACGCCATACCAATTTTCTGGAATTTGATTAATAATCTCTTTAGGTTCGGCAGTTGCAAATACAGAATAGCCTTCTTGTTGTAATAAATCGGCATACGATTCCaaaa                                                 >  1:336973/1‑224 (MQ=255)                                                                                                                                                                                                                              gAACATCACCATGCCCGGTAATCATAATCACCGGAATTTGGCTATCCATTTCAATCATTCGATCTAATCATCTAATCCTGAAATGCCCAGCAATAATACGTCGCAAATCACAACGCCATACCAATTTTCTGGAATTTGATTAATAATCTCTTTAGGTTCGGCAGTTGCAAATACAGAATAGCCTTCTTGTTGTAATAAATCGGCATACGATTCCAAAATATCAAAATCATCATCAATTAATAAAAc                     >  1:251361/1‑246 (MQ=255)                                                                                                                                                                                                                              gAACATCACCATGCCCGGTAATCATAATCACCGGAATTTGGCTATCCATTTCAATCATTCGATCTAATCATCTAATCCTGAAATGCCCAGCAATAATACGTCGCAAATCACAACGCCATACCAATTTTCTGGAATTTGATTAATAATCTCTTTAGGTTAGGCAGTTGCAAATACAGAATAGCCTTCTTGTTGTAATAAATCGGCATAc                                                           >  1:710679/1‑208 (MQ=255)                                                                                                                                                                                                                                 cATCACCATGCCCGGTAATCATAATCACCGGAATTTGGCTATCCATTTCAATCATTCGATCTAATCATCTAATCCTGAAATGCCCAGCAATAATACGTCGCAAATCACAACGCCATACCAATTTTCTGGAATTTGATTAATAATCTCTTTAGGTTCGGCAGTTGCAAATCCAGAATAGCCTTCTTGTTGTAATAAATCGGCATACGATTCCAAAATATCAAAATCATCATCAATTAATAAAAc                     >  1:449481/1‑243 (MQ=255)                                                                                                                                                                                                                                          gCCCGGTAATCATAATCACCGGAATTTGGCTATCCATTTCAATCATTCGATCTAATCATCTAATCCTGAAATGCCCAGCAATAATACGTCGCAAATCACAACGCCATACCAATTTTCTGGAATTTGATTAATAATCTCTTTAgg                                                                                                               >  1:175362/1‑144 (MQ=255)                                                                                                                                                                                                                                          gCCCGGTAATCATAATCACCGGAATTTGGCTATCCATTTCAATCATTCGATCTAATCATCTAATCCTGAAATGCCCAGCAATAATACGTCGCAAATCACAACGCCATACCAATTTTCTGGAATTTGATTAATAATCTCTTTAGGTTCGGCAGTTGCAAATACAGAATAGCCTTCTTGTTGTAATAAATCGGCATACGATTCCAAAATATCAAAATCATCATCAATTAATAAAACATTATTCTGATAATTaa    >  1:862093/1‑251 (MQ=255)                                                                                                                                                                                                                                            ccGGTAATCATAATCACCGGAATTTGGCTATCCATTTCAATCATTCGATCTAATCATCTAATCCTGAAATGCCCAGCAATAATACGTCGCAAATCACAACGCCATACCAATTTTCTGGAATTTGATTAATAATCTCTTTAGGTTCGGCAGTTGCAAATACAGAATAGCCTTCTTGTTGTAATAAATCGGCATACGATTCCAAAATATCAAAATCATCATCAATTAATAAAACATTATTCTGATAATTAAAc  <  1:641197/251‑1 (MQ=255)                                                                                                                                                                                                                                                  atcataatcacCGGAATTTGGCTATCCATTTCAATCATTCGATCTAATCATCTAATCCTGAAATGCCCAGCAATAATACGTCGCAAATc                                                                                                                                                              >  1:449678/1‑89 (MQ=255)                                                                                                                                                                                                                                                  atcataatcacCGGAATTTGGCTATCCATTTCAATCATTCGATCTAATCATCTAATCCTGAAATGCCCAGCAATAATACGTCGCAAATCACAACGCCATACCAATTTTCTGGAATTTGATTAATAATCTCTTTAGGt                                                                                                              <  1:47022/137‑1 (MQ=255)                                                                                                                                                                                                                                                  atcataatcacCGGAATTTGGCTATCCATTTCAATCATTCGATCTAATCATCTAATCCTGAAATGCCCAGCAATAATACGTCGCAAATCACAACGCCATACCAATTTTCTGGAATTTGATTAATAATCTCTTTAGGTTCGGCAGTTGCAAATACAGAATAGCct                                                                                   >  1:834136/1‑164 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                 ATTCGAATTGGCCAGCTTCTGTAATTGTTTTTTTAACTCGTTAATCCAAACACTGCTGCCAATAAAAACGTCATTAATTTTTTCCATTTGCCATTGGCGCTTTTCAATCACCTTTTTCCGTTTTTTTAAAGCGTTATCGACTTGAATAAGTAATTTTTCAGGGGAAACCGGTTTTTCAAAGAAATTTGTCGCCCCTTTCTTGACAGCTTCTACCGCCATGGGAACATCACCATGCCCGGTAATCACAATCACCGGAATTTGGCTATCCATTTCAATCATTCGATCTAATCATCTAATCCTGAAATGCCCAGCAATAATACGTCGCAAATCACAACGCCATACCAATTTTCTGGAATTTGATTAATAATCTCTTTAGGTTCGGCAGTTGCAAATACAGAATAGCCTTCTTGTTGTAATAAATCGGCATACGATTCCAAAATATCAAAATCATCATCAATTAATAAAACATTATTCTGATAATTAAAC  >  1/1594690‑1595175

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑