Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,498,379	A→G	A353A (GCA→GCG)	unknown →	Ribosome‑associated ATPase RbbA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,498,379	0	A	G	100.0%	269.0 / NA	78	A353A (GCA→GCG)	unknown	Ribosome‑associated ATPase RbbA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (35/43);  total (35/43)

GTTATCGTTGCCAAAGGTTTAACCAAGAAATTCGGTGATTTCGTTTCGGTGGATAACGTCAGTTTTACCATTCCGAAAGGAGAAATTTTCGGCTTTTTAGGCTCCAACGGTTGCGGCAAATCCACCACCATGAAAATGCTTACCGGCTTGCTGGATCCGACGGAAGGCTCTGCCACGCTGCTCGGTCAGCCTATTGATGCGGGCAATATCGACACGCGAAAACGGGTGGGTTATATGTCCCAAGCATTTTCCTTATATGAAGAACTCAGCGTTTATCAAAATCTGGAATTGCACGCCAAGCTCTATCAAACTCCCCGCGCGCAATGGGCACACTATGTGCAATCCGTCATGCAGCAATTCGATTTAATTCAACTTGCCGATGAATACCCTTCCGCGCTGCCTCTTGGCATTCGGCAACGTTTGCAGCTTGCCGCCGCCTGTTTGCATAAACCCGAAGTGCTGATTCTGGACGAACCTACTTCAGGGG  >  1/1498134‑1498620                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                  gTTATCGTTGCCAAAGGTTTAACCAAGAAATTCGGTGATTTCGTTTCGGTGGATAACGTCAGTTTTACCATTCCGAAAGGAGAAATTTTCGGCTTTTTAGGCTCCAACGGTTGCGGCAAATCCACCACCATGAAAATGCTTACCGGCTTGCTGGATCCGACGGAAGGCTCTGCCACGCTGCTCGGTCAGCCTATTGATGCGGGCAATATCGACACACGAAAACGGGTGGGTTATATGTCCCAAGCGtttt                                                                                                                                                                                                                                               >  1:50921/1‑250 (MQ=255)  ttATCGTTGCCAAAGGTTTAACCAAGAAATTCGGTGATTTCGTTTCGGTGGATAACGTCAGTTTTACCATTCCGAAAGGAGAAATTTTCGGCTTTTTAGGCTCCAACGGTTGCGGCAAATCCACCACCATGAAAATGCTTACCGGCTTGCTGGATCCGACGGAAGGCTCTGCCACGCTGCTCGGTCAGCCTATTGATGCGGGCAATATCGACACGCGAAAACGGGTGGGTTATATGTCCCAAGCGTTTTcc                                                                                                                                                                                                                                             <  1:807561/251‑1 (MQ=255)      cGTTGCCAAAGGTTTAACCAAGAAATTCGGTGATTTCGTTTCGGTGGATAACGTCAGTTTTACCATTCCGAAAGGAGAAATTTTCGGCTTTTTAGGCTCCAGCGGTTGCGGCAAATCCACCACCATGAAAATGCTTTCCGGCTTGCTGGATCCGACGGAAGGCTCTGCCACGCTGCTCGGTCAGCCTATTGATGCGGGCAATATCGACACGCGAAAACGGGTGGGTTATATGTCCCAAGCGTTTTCCTtat                                                                                                                                                                                                                                         <  1:713152/251‑1 (MQ=255)             aaaGGTTTAACCAAGAAATTCGGTGATTTCGTTTCGGTGGATAACGTCAGTTTTACCATTCCGAAAGGAGAAATTTTCGGCTTTTTAGGCTCCAACGGTTGCGGCAAATCCACCACCATGAAAATGCTTACCGGCTTGCTGGATCCGACGGAAGGCTCTGCCACGCTGCTCGGTCAGCCTATTGATGCGGGCAATATCGACACGCGAAAACGGGTGGGTTATATGTCCCAAGCGTTTTCCTTATATgaaga                                                                                                                                                                                                                                  <  1:680803/251‑1 (MQ=255)                ggTTTAACCAAGAAATTCGGTGATTTCGTTTCGGTGGATAACGTCAGTTTTACCATTCCGAAAGGAGAAATTTTCGGCTTTTTAGGCTCCAACGGTTGCGGCAAATCCACCACCATGAAAATGCTTACCGGCTTGCTGGATCCGACGGAAGGCTCTGCCACGCTGCTCGGTCAGCCTATTGATGCGGGCAATATCGACACGCGAAAACGGGTGGGTTATATGTCCCAAGCGTTTTCCTTATATgaagaa                                                                                                                                                                                                                                 >  1:408257/1‑249 (MQ=255)                ggTTTAACCAAGAAATTCGGTGATTTCGTTTCGGTGGATAACGTCAGTTTTACCATTCCGAAAGGAGAAATTTTCGGCTTTTTAGGCTCCAACGGTTGCGGCAAATCCACCACCATGAAAATGCTTACCGGCTTGCTGGATCCGACGGAAGGCTCTGCCACGCTGCTCGGTCAGCCTATTGATGCGGGCAATATCGACACGCGAAAACGGGTGGGTTATATGTCCCAAGCGTTTTCCTTATATGAAGAAc                                                                                                                                                                                                                                <  1:686455/250‑1 (MQ=255)                 gTTTAACCAAGAAATTCGGTGATTTCGTTTCGGTGGATAACGTCAGTTTTACCATTCCGAAAGGAGAAATTTTCGGCTTTTTAGGCTCCAACGGTTGCGGCAAATCCGCCGCCATGAAAATGCTTGCCGGCTTGCTGGATCCGACGGAAGGCTCTGCCACGCTGCTCGGTCAGCCTATTGATGCGGGCAATATCGACACGCGAAAACGGGTGGGTTATATGTCCCAAGCGTTTTCCTTATATGAAGAAc                                                                                                                                                                                                                                <  1:853973/249‑1 (MQ=255)                                               cgGTGGATAACGTCAGTTTTACCATTCCGAAAGGAGAAATTTTCGGCTTTTTAGGCTCCAACGGTTGCGGCAAATCCACCACCATGAAAATGCTTACCGGCTTGCTGGATCCGACGGAAGGCTCTGCCACGCTGCTCGGTCAGCCTATTGATGCGGGCAATATCGACACGCGAAAACGGGTGGGTTATATGTCCCAAGCGTTTTCCTTATATGAAGAACTCAGCGTTTATCAAAATCTGGAATTGCACGcc                                                                                                                                                                                                <  1:31285/251‑1 (MQ=255)                                                   ggATAACGTCAGTTTTACCATTCCGAAAGGAGAAATTTTCGGCTTTTTAGGCTCCAACGGTTCCGGCAAATCCACCACCATGAAAATGCTTACCGGCTTGCTGGATCCGACGGAAGGCTCTGCCACGCTGCTCGGTCAGCCTATTGATGCGGGCAATATCGACACGCGAAAACGGGTGGGTTATATGTCCCAAGCGTTTTCCTTATATGAAGAACTCAGCGTTTATCAAAATCTGGAATTGCACGCCAAg                                                                                                                                                                                             >  1:661090/1‑250 (MQ=255)                                                     aTAACGTCAGTTTTACCATTCCGAAAGGAGAAATTTTCGGCTTTTTAGGCTCCAACGGTTGCGGCAAATCCACCACCATGAAAATGCTTACCGGCTTGCTGGATCCGACGGAAGGCTCTGCCACGCTGCTCGGTCAGCCTATTGATGCGGGCAATATCGACACGCGAAAACGGGTGGGTTATATGTCCCAAGCGTTTTCCTTATATGAAGAACTCAGCGTTTATCAAAATCTGGAATTGCACGCCAAGctc                                                                                                                                                                                          <  1:668613/251‑1 (MQ=255)                                                        aCGTCAGTTTTACCATTCCGAAAGGAGAAATTTTCGGCTTTTTAGGCTCCAACGGTTGCGGCAAATCCACCACCATGAAAATGCTTACCGGCTTGCTGGATCCGACGGAAGGCTCTGCCACGCTGCTCGGTCAGCCTATTGATGCGGGCAATATCGACACGCGAAAACGGGTGGGTTATATGTCCCAAGCGTTTTCCTTATATGAAGAACTCAGCGTTTATCAAAATCTGGAATTGCACGCCAAGCTCTAt                                                                                                                                                                                       <  1:794128/251‑1 (MQ=255)                                                              gTTTTACCATTCCGAAAGGAGAAATTTTCGGCTTTTTAGGCTCCAACGGTTGCGGCAAATCCACCACCATGAAAATGCTTACCGGCTTGCTGGATCCGACGGAAGGCTCTGCCACGCTGCTCGGTCAGCCTATTGATGCGGGCAATATCGACACGCGAAAACGGGTGGGTTATATGTCCCAAGCGTTTTCCTTATATGAAGAACTCAGCGTTTATCAAAATCTGGAATTGCACGCCAAGCTCTATCaaa                                                                                                                                                                                   >  1:434869/1‑249 (MQ=255)                                                               ttttACCATTCCGAAAGGAGAAATTTTCGGCTTTTTAGGCTCCAACGGTTGCGGCAAATCCACCACCATGAAAATGCTTACCGGCTTGCTGGATCCGACGGAAGGCTCTGCCACGCTGCTCGGTCAGCCTATTGATGCGGGCAATATCGACACGCGAAAACGGGTGGGTTATATGTCCCAAGCGTTTTCCTTATATGAAGAACTCAGCGTTTATCAAAATCTGGAATTGCACGCCAAGCTCTATCAAAc                                                                                                                                                                                  <  1:766143/249‑1 (MQ=255)                                                               ttttACCATTCCGAAAGGAGAAATTTTCGGCTTTTTAGGCTCCAACGGTTGCGGCAAATCCACCACCATGAAAATGCTTACCGGCTTGCTGGATCCGACGGAAGGCTCTGCCACGCTGCTCGGTCAGCCTATTGATGCGGGCAATATCGACACGCGAAAACGGGTGGGTTATATGTCCCAAGCGTTTTCCTTATATGAAGAACTCAGCGTTTATCAAAATCTGGAATTGCACGCCAAGCTCTATCAAAc                                                                                                                                                                                  <  1:277122/249‑1 (MQ=255)                                                               ttttACCATTCCGAAAGGAGAAATTTTCGGCTTTTTAGGCTCCAACGGTTGCGGCAAATCCACCACCATGAAAATGCTTACCGGCTTGCTGGATCCGACGGAAGGCTCTGCCACGCTGCTCGGTCAGCCTATTGATGCGGGCAATATCGACACGCGAAAACGGGTGGGTTATATGTCCCAAGCGTTTTCCTTATATGAAGAACTCAGCGTTTATCAAAATCTGGAATTGCACGCCAAGCTCTATCAAACTc                                                                                                                                                                                <  1:530379/251‑1 (MQ=255)                                                               ttttACCATTCCGAAAGGAGAAATTTTCGGCTTTTTAGGCTCCAACGGTTGCGGCAAATCCACCACCATGAAAATGCTTACCGGCTTGCTGGATCCGACGGAAGGCTCTGCCACGCTGCTCGGTCAGCCTATTGATGCGGGCAATATCGACACGCGAAAACGGGTGGGTTATATGTCCCAAGCGTTTTCCTTATATGAAGAACTCAGCGTTTATCAAAATCTGGAATTGCACGCCAAGCTCTATCAAACTc                                                                                                                                                                                <  1:520689/251‑1 (MQ=255)                                                                   aCCATTCCGAAAGGAGAAATTTTCGGCTTTTTAGGCTCCAACGGTTGCGGCAAATCCACCACCATGAAAATGCTTACCGGCTTGCTGGATCCGACGGAAGGCTCTGCCACGCTGCTCGGTCAGCCTATTGATGCGGGCAATATCGACACGCGAAAACGGGTGGGTTATATGTCCCAAGCGTTTTCCTTATATGAAGAACTCAGCGTTTATCAAAATCTGGAATTGCACGCCAAGCTCTATCAAACTCCCcg                                                                                                                                                                            >  1:555598/1‑251 (MQ=255)                                                                      aTTCCGAAAGGAGAAATTTTCGGCTTTTTAGGCTCCAACGGTTGCGGCAAATCCACCACCATGAAAATGCTTACCGGCTTGCTGGATCCGACGGAAGGCTCTGCCACGCTGCTCGGTCAGCCTATTGATGCGGGCAATATCGACACGCGAAAACGGGTGGGTTATATGTCCCAAGCGTTTTCCTTATATGAAGAACTCAGCGTTTATCAAAATCTGGAATTGCACGCCAAGCTCTATCAAACTCCCcgcgc                                                                                                                                                                         <  1:6104/251‑1 (MQ=255)                                                                                gagaAATTTTCGGCTTTTTAGGCTCCAACGGTTGCGGCAAATCCACCACCATGAAAATGCTTACCGGCTTGCTGGATCCGACGGAAGGCTCTGCCACGCTGCTCGGTCAGCCTATTGATGCGGGCAATATCGACACGCGAAAACGGGTGGGTTATATGTCCCAAGCGTTTTCCTTATATGAAGAACTCAGCGTTTATCAAAATCTGGAATTGCACGCCAAGCTCTATCAAACTCCCCGCGCGCAATGGGc                                                                                                                                                                <  1:498411/250‑1 (MQ=255)                                                                                    aaTTTTCGGCTTTTTAGGCTCCAACGGTTGCGGCAAATCCACCACCATGAAAATGCTTACCGGCTTGCTGGATCCGACGGAAGGCTCTGCCACGCTGCTCGGTCAGCCTATTGATGCGGGCAATATCGACACGCGAAAACGGGTGGGTTATATGTCCCAAGCGTTTTCCTTATATGAAGAACTCAGCGTTTATCAAAATCTGGAATTGCACGCCAAGCTCTATCAAACTCCCCGCGCGCAATGGGCACACt                                                                                                                                                           <  1:480225/251‑1 (MQ=255)                                                                                         tCGGCTTTTTAGGCTCCAACGGTTGCGGCAAATCCACCACCATGAAAATGCTTACCGGCTTGCTGGATCCGACGGAAGGCTCTGCCACGCTGCTCGGTCAGCCTATTGATGCGGGCAATATCGACACGCGAAAACGGGTGGGTTATATGTCCCAAGCGTTTTCCTTATATGAAGAACTCAGCGTTTATCAAAATCTGGAATTGCACGCCAAGCTCTATCAAACTCCCCGCGCGCAATGGGCACACTAtgtg                                                                                                                                                      <  1:49917/251‑1 (MQ=255)                                                                                          cGGCTTTTTAGGCTCCAACGGTTGCGGCAAATCCACCACCATGAAAATGCTTACCGGCTTGCTGGATCCGACGGAAGGCTCTGCCACGCTGCTCGGTCAGCCTATTGATGCGGGCAATATCGACACGCGAAAACGGGTGGGTTATATGTCCCAAGCGTTTTCCTTATATGAAGAACTCAGCGTTTATCAAAATCTGGAATTGCACGCCAAGCTCTATCAAACTCCCCGCGCGCAATGGGCACACTATGTGc                                                                                                                                                     <  1:700438/251‑1 (MQ=255)                                                                                          cGGCTTTTTAGGCTCCAACGGTTGCGGCAAATCCACCACCATGAAAATGCTTACCGGCTTGCTGGATCCGACGGAAGGCTCTGCCACGCTGCTCGGTCAGCCTATTGATGCGGGCAATATCGACACGCGAAAACGGGTGGGTTATATGTCCCAAGCGTTTTCCTTATATGAAGAACTCAGCGTTTATCAAAATCTGGAATTGCACGCCAAGCTCTATCAAACTCCCCGCGCGCAATGGGCACACTATGTGc                                                                                                                                                     <  1:238261/251‑1 (MQ=255)                                                                                              tttttAGGCTCCAACGGTTGCGGCAAATCCACCACCATGAAAATGCTTACCGGCTTGCTGGATCCGACGGAAGGCTCTGCCACGCTGCTCGGTCAGCCTATTGATGCGGGCAATATCGACACGCGAAAACGGGTGGGTTATATGTCCCAAGCGTTTTCCTTATATGAAGAACTCAGCGTTTATCAAAATCTGGAATTGCACGCCAAGCTCTATCAAACTCCCCGCGCGCAATGGGCACACTAtgtg                                                                                                                                                      <  1:365701/246‑1 (MQ=255)                                                                                              tttttAGGCTCCAACGGTTGCGGCAAATCCACCACCATGAAAATGCTTACCGGCTTGCTGGATCCGACGGAAGGCTCTGCCACGCTGCTCGGTCAGCCTATTGATGCGGGCAATATCGACACGCGAAAACGGGTGGGTTATATGTCCCAAGCGTTTTCCTTATATGAAGAACTCAGCGTTTATCAAAATCTGGAATTGCACGCCAAGCTCTATCAAACTCCCCGCGCGCAATGGGCACACTATGTGCAAt                                                                                                                                                  >  1:623756/1‑250 (MQ=255)                                                                                                     gCTCCAACGGTTGCGGCAAATCCACCACCATGAAAATGCTTACCGGCTTGCTGGATCCGACGGAAGGCTCTGCCACGCTGCTCGGTCAGCCTATTGATGCGGGCAATATCGACACGCGAAAACGGGTGGGTTATATGTCCCAAGCGTTTTCCTTATATGAAGAACTCAGCGTTTATCAAAATCTGGAATTGCACGCCAAGCTCTATCAAACTCCCCGCGCGCAATGGGCACACTATGTGCAATCCGTCAt                                                                                                                                           <  1:648538/250‑1 (MQ=255)                                                                                                        ccAACGGTTGCGGCAAATCCACCACCATGAAAATGCTTACCGGCTTGCTGGATCCGACGGAAGGCTCTGCCACGCTGCTCGGTCAGCCTATTGATGCGGGCAATATCGACACGCGAAAACGGGTGGGTTATATGTCCCAAGCGTTTTCCTTATATGAAGAACTCAGCGTTTATCAAAATCTGGAATTGCACGCCAAGCTCTATCAAACTCCCCGCGCGCAATGGGCACACTATGTGCAATCCGTCATgcag                                                                                                                                       <  1:648585/251‑1 (MQ=255)                                                                                                        ccAACGGTTGCGGCAAATCCACCACCATGAAAATGCTTACCGGCTTGCTGGATCCGACGGAAGGCTCTGCCACGCTGCTCGGTCAGCCTATTGATGCGGGCAATATCGACACGCGAAAACGGGTGGGTTATATGTCCCAAGCGTTTTCCTTATATGAAGAACTCAGCGTTTATCAAAATCTGGAATTGCACGCCAAGCTCTATCAAACTCCCCGCGCGCAATGGGCACACTATGTGCAATCCGTCATgcag                                                                                                                                       >  1:899960/1‑251 (MQ=255)                                                                                                           aCGGTTGCGGCAAATCCACCACCATGAAAATGCTTACCGGCTTGCTGGATCCGACGGAAGGCTCTGCCACGCTGCTCGGTCAGCCTATTGATGCGGGCAATATCGACACGCGAAAACGGGTGGGTTATATGTCCCAAGCGTTTTCCTTATATGAAGAACTCAGCGTTTATCAAAATCTGGAATTGCACGCCAAGCTCTATCAAACTCCCCGCGCGCAATGGGCACACTATGTGCAATCCGTCATGCAGCaa                                                                                                                                    <  1:779564/251‑1 (MQ=255)                                                                                                                  cGGCAAATCCACCACCATGAAAATGCTTACCGGCTTGCTGGATCCGACGGAAGGCTCTGCCACGCTGCTCGGTCAGCCTATTGATGCGGGCAATATCGACACGCGAAAACGGGTGGGTTATATGTCCCAAGCGTTTTCCTTATATGAAGAACTCAGCGt                                                                                                                                                                                                                         <  1:28473/159‑1 (MQ=255)                                                                                                                        aTCCACCACCATGAAAATGCTTACCGGCTTGCTGGATCCGACGGAAGGCTCTGCCACGCTGCTCGGTCAGCCTATTGATGCGGGCAATATCGACACGCGAAAACGGGTGGGTTATATGTCCCAAGCGTTTTCCTTATATGAAGAACTCAGCGTTTATCAAAATCTGGAATTGCACGCCAAGCTCTATCAAACTCCCCGCGCGCAATGGGCACACTAtgtg                                                                                                                                                      >  1:272278/1‑220 (MQ=255)                                                                                                                        aTCCACCACCATGAAAATGCTTACCGGCTTGCTGGATCCGACGGAAGGCTCTGCCACGCTGCTCGGTCAGCCTATTGATGCGGGCAATATCGACACGCGAAAACGGGTGGGTTATATGTCCCAAGCGTTTTCCTTATATGAAGAACTCAGCGTTTATCAAAATCTGGAATTGCACGCCAAGCTCTATCAAACTCCCCGCGCGCAATGGGCACACTATGTGCaa                                                                                                                                                   <  1:592404/223‑1 (MQ=255)                                                                                                                        aTCCACCACCATGAAAATGCTTACCGGCTTGCTGGATCCGACGGAAGGCTCTGCCACGCTGCTCGGTCAGCCTATTGATGCGGGCAATATCGACACGCGAAAACGGGTGGGTTATATGTCCCAAGCGTTTTCCTTATATGAAGAACTCAGCGTTTATCAAAATCTGGAATTGCACGCCAAGCTCTATCAAACTCCCCGCGCGCAATGGGCACACTATGTGCAATcc                                                                                                                                                <  1:539221/226‑1 (MQ=255)                                                                                                                                   tGAAAATGCTTACCGGCTTGCTGGATCCGACGGAAGGCTCTGCCACGCTGCTCGGTCAGCCTATTGATGCGGGCAATATCGACACGCGAAAACGGGTGGGTTATATGTCCCAAGCGTTTTCCTTATATGAAGAACTCAGCGTTTATCAAAATCTGGAATTGCACGCCAAGCTCTATCAAACTCCCCGCGCGCAATGGGCACACTATGTGCAATCCGTCATGCAGCAATTCGATTTAATTCAACTTGCCGAt                                                                                                            >  1:487909/1‑251 (MQ=255)                                                                                                                                       aaTGCTTACCGGCTTGCTGGATCCGACGGAAGGCTCTGCCACGCTGCTCGGTCAGCCTATTGATGCGGGCAATATCGACACGCGAAAACGGGTGGGTTATATGTCCCAAGCGTTTTCCTTATATGAAGAACTCAGCGTTTATCAAAATCTGGAATTGCACGCCAAGCTCTATCAAACTCCCCGCGCGCAATGGGCACACTATGTGCAATCCGTCATGCAGCAATTCGATTTAATTCAACTTGCCGATGAAt                                                                                                        >  1:398956/1‑251 (MQ=255)                                                                                                                                         ttgacggcgggagagcggATCCGACGGAAGGCTCTGCCACGCTGCTCGGTCAGCCTATTGATGCGGGCAATATCGACACGCGAAAACGGGTGGGTTATATGTCCCAAGCGTTTTCCTTATATGAAGAACTCAGCGTTTATCAAAATCTGGAATTGCACGCCAAGCTCTATCAAACTCCCCGCGCGCAATGGGCACACTATGTGCAATCCGTCATGCAGCAATTCGATTTAATTCAACTTGCCGATGAATAc                                                                                                      <  1:437752/235‑1 (MQ=255)                                                                                                                                            ttACCGGCTTGCTGGATCCGACGGAAGGCTCTGCCACGCTGCTCGGTCAGCCTATTGATGCGGGCAATATCGACACGCGAAAACGGGTGGGTTATATGTCCCAAGCGTTTTCCTTATATGAAGAACTCAGCGTTTATCAAAATCTGGAATTGCACGCCAAGCTCTATCAAACTCCCCGCGCGCAATGGGCACACTATGTGCAATCCGTCATGCAGCAATTCGATTTAATTCAACTTGCCGATGAATAcc                                                                                                     >  1:211255/1‑249 (MQ=255)                                                                                                                                                 ggCTTGCTGGATCCGACGGAAGGCTCTGCCACGCTGCTCGGTCAGCCTATTGATGCGGGCAATATCGACACGCGAAAACGGGTGGGTTATATGTCCCAAGCGTTTTCCTTATATGAAGAACTCAGCGTTTATCAAAATCTGGAATTGCACGCCAAGCTCTATCAAACTCCCCGCGCGCAATGGGCACACTAtgtg                                                                                                                                                      >  1:251471/1‑195 (MQ=255)                                                                                                                                                         ggATCCGACGGAAGGCTCTGCCACGCTGCTCGGTCAGCCTATTGATGCGGGCAATATCGACACGCGAAAACGGGTGGGTTATATGTCCCAAGCGTTTTCCTTATATgaag                                                                                                                                                                                                                                   <  1:391077/110‑1 (MQ=255)                                                                                                                                                         ggATCCGACGGAAGGCTCTGCCACGCTGCTCGGTCAGCCTATTGATGCGGGCAATATCGACACGCGAAAACGGGTGGGTTATATGTCCCAAGCGTTTTCCTTATATGAAGAACTCAGCGTTTATCAAAATCTGGAATTGCACGCCAAGCTCTATCAAACTCCCCGCGCGCAATGGGCACACTATGTGCAATCCGTCATGCAGCAATTCGATTTAATTCAACTTGCCGATGAATACCCTTCCGCGCTGCct                                                                                       >  1:768262/1‑250 (MQ=255)                                                                                                                                                                       gCTCTGCCACGCTGCTCGGTCAGCCTATTGATGCGGGCAATATCGACACGCGAAAACGGGTGGGTTATATGTCCCAAGCGTTTTCCTTATATGAAGAACTCAGCGTTTATCAAAATCTGGAATTGCACGCCAAGCTCTATCAAACTCCCcg                                                                                                                                                                            <  1:608591/151‑1 (MQ=255)                                                                                                                                                                       gCTCTGCCACGCTGCTCGGTCAGCCTATTGATGCGGGCAATATCGACACGCGAAAACGGGTGGGTTATATGTCCCAAGCGTTTTCCTTATATGAAGAACTCAGCGTTTATCAAAATCTGGAATTGCACGCCAAGCTCTATCAAACTCCCCGCGCGCAATGGGCACACTATGTGCAAt                                                                                                                                                  <  1:262376/177‑1 (MQ=255)                                                                                                                                                                       gCTCTGCCACGCTGCTCGGTCAGCCTATTGATGCGGGCAATATCGACACGCGAAAACGGGTGGGTTATATGTCCCAAGCGTTTTCCTTATATGAAGAACTCAGCGTTTATCAAAATCTGGAATTGCACGCCAAGCTCTATCAAACTCCCCGCGCGCAATGGGCACACTATGTGCAATCCGTCATGCAGCAATTCGATTTAATTCAACTTGCCGATGAATACCCTTCCGCGCTGCCTCTTGGCATTCGGCaa                                                                        >  1:29490/1‑251 (MQ=255)                                                                                                                                                                         tctGCCACGCTGCTCGGTCAGCCTATTGATGCGGGCAATATCGACACGCGAAAACGGGTGGGTTATATGTCCCAAGCGTTTTCCTTATATGAAGAACTCAGCGTTTATCAAAATCTGGAATTGCACGCCAAGCTCTATCAAACTCCCCGCGCGCAATGGGCACACTATGTGCAATCCGTCATGCAGCAATTCGATTTAATTCAACTTGCCGATGAATACCCTTCCGCGCTGCCTCTTGGCATTCGGCAACg                                                                      <  1:214212/251‑1 (MQ=255)                                                                                                                                                                          ctGCCACGCTGCTCGGTCAGCCTATTGATGCGGGCAATATCGACACGCGAAAACGGGTGGGTTATATGTCCCAAGCGTTTTCCTTATATGAAGAACTCAGCGTTTATCAAAATCTGGAATTGCACGCCAAGCTCTATCAAACTCCCCGCGCGCAATGGGCACACTATGTGCAATCCGTCATGCAGCAATTCGATTTAATTCAACTTGCCGATGAATACCCTTCCGCGCTGCCTCTTGGCATTCGGCAACGt                                                                     >  1:822091/1‑251 (MQ=255)                                                                                                                                                                               aCGCTGCTCGGTCAGCCTATTGATGCGGGCAATATCGACACGCGAAAACGGGTGGGTTATATGTCCCAAGCGTTTTCCTTATATGAAGAACTCAGCGTTTATCAAAATCTGGAATTGCACGCCAAGCTCTATCAAAc                                                                                                                                                                                  <  1:13508/137‑1 (MQ=255)                                                                                                                                                                                    gctCGGTCAGCCTATTGATGCGGGCAATATCGACACGCGAAAACGGGTGGGTTATATGTCTCAAGCGTTTTCCTTATATGAAGAACTCAGCGTTTATCAAAATCTGGAATTGCACGCCAAGCTCTATCAAACTCCCCGCGCGCAATGGGCACACTATGTGCAATCCGTCATGCAGCAATTCGATTTAATTCAACTTCCCGATGAATACCCTTCCGCGCTGCCTCTTGGCa                                                                                <  1:455581/230‑1 (MQ=255)                                                                                                                                                                                         gTCAGCCTATTGATGCGGGCAATATCGACACGCGAAAACGGGTGGGTTATATGTCCCAAGCGTTTTCCTTATATGAAGAACTCAGCGTTTATCAAAATCTGGAATTGCACGCCAAGCTCTATCAAACTCCCCGCGCGCAATGGGCACACTATGTGCAATCCGTCATGCAGCAATTCGATTTAATTCAACTTGCCGATGAATACCCTTCCGCGCTGCCTCTTGGCATTCGGCAACGTTTGCAGCTTgccgc                                                       >  1:356601/1‑250 (MQ=255)                                                                                                                                                                                             gCCTATTGATGCGGGCAATATCGACACGCGAAAACGGGTGGGTTATATGTCCCAAGCGTTTTCCTTATATGAAGAACTCAGCGTTTATCAAAATCTGGAATTGCACGCCAAGct                                                                                                                                                                                           >  1:105069/1‑114 (MQ=255)                                                                                                                                                                                             gCCTATTGATGCGGGCAATATCGACACGCGAAAACGGGTGGGTTATATGTCCCAAGCGTTTTCCTTATATGAAGAACTCAGCGTTTATCAAAATCTGGAATTGCACGCCAAGCTCTATCAAACTCCCCGCGCGCAATGGGCACACTATGTGCAATCCGTCATgc                                                                                                                                         <  1:614657/164‑1 (MQ=255)                                                                                                                                                                                             gCCTATTGATGCGGGCAATATCGACACGCGAAAACGGGTGGGTTATATGTCCCAAGCGTTTTCCTTATATGAAGAACTCAGCGTTTATCAAAATCTGGAATTGCACGCCAAGCTCTATCAAACTCCCCGCGCGCAATGGGCACACTATGTGCAATCCGTCATGCAGCAATTCGATTTAATTCAACTTGCCGATGAATACCCTTCCGCGCTGCCTCTTGGCATTCGGCAACGTTTGCAGCTTgccgccgc                                                    >  1:111465/1‑249 (MQ=255)                                                                                                                                                                                             gCCTATTGATGCGGGCAATATCGACACGCGAAAACGGGTGGGTTATATGTCCCAAGCGTTTTCCTTATATGAAGAACTCAGCGTTTATCAAAATCTGGAATTGCACGCCAAGCTCTATCAAACTCCCCGCGCGCAATGGGCACACTATGTGCAATCCGTCATGCAGCAATTCGATTTAATTCAACTTGCCGATGAATACCCTTCCGCGCTGCCTCTTGGCATTCGGCAACGTTTGCAGCTTgccgccgc                                                    >  1:687933/1‑249 (MQ=255)                                                                                                                                                                                             gCCTATTGATGCGGGCAATATCGACACGCGAAAACGGGTGGGTTATATGTCCCAAGCGTTTTCCTTATATGAAGAACTCAGCGTTTATCAAAATCTGGAATTGCACGCCAAGCTCTATCAAACTCCCCGCGCGCAATGGGCACACTATGTGCAATCCGTCATGCAGCAATTCGATTTAATTCAACTTGCCGATGAATACCCTTCCGCGCTGCCTCTTGGCATTCGGCAACGTTTGCAGCTTgccgccgc                                                    >  1:677696/1‑249 (MQ=255)                                                                                                                                                                                               cTATTGATGCGGGCAATATCGACACGCGAAAACGGGTGGGTTATATGTCCCAAGCGTTTTCCTTATATGAAGAACTCAGCGTTTATCAAAATCTGGAATTGCACGCCAAGCTCTATCAAACTCCCCGCGCGCAATGGGCACACTATGTGCAATCCGTCATGCAGCAATTCGATTTAATTCAACTTGCCGATGAATACCCTTCCGCGCTGCCTCTTGGCATTCGGCAACGTTTGCAGCTTgccgccgc                                                    >  1:271495/1‑247 (MQ=255)                                                                                                                                                                                               cTATTGATGCGGGCAATATCGACACGCGAAAACGGGTGGGTTATATGTCCCAAGCGTTTTCCTTATATGAAGAACTCAGCGTTTATCAAAATCTGGAATTGCACGCCAAGCTCTATCAAACTCCCCGCGCGCAATGGGCACACTATGTGCAATCCGTCATGCAGCAATTCGATTTAATTCAACTTGCCGATGAATACCCTTCCGCGCTGCCTCTTGGCATTCGGCAACGTTTGCAGCTTgccgccgc                                                    >  1:858788/1‑247 (MQ=255)                                                                                                                                                                                                    gATGCGGGCAATATCGACACGCGAAAACGGGTGGGTTATATGTCCCAAGCGTTTTCCTTATATGAAGAACTCAGCGTTTATCAAAATCTGGAATTGCACGCCAAGCTCTATCAAACTCCCCGCGCGCAATGGGCACACTATGTGCAATCCGTCATGCAGCAATTCGATTTAATTCAACTTGCCGATGAATACCCTTCCGCGCTGCCTCTTGGCATTCGGCAACGTTTGCAGCTTGCCGCCGCCTGTTTGCa                                           >  1:788533/1‑251 (MQ=255)                                                                                                                                                                                                          ggCAATATCGACACGCGAAAACGGGTGGGTTATATGTCCCAAGCGTTTTCCTTATATGAAGAACTCAGCGTTTATCAAAATCTGGAATTGCACGCCAAGCTCTATCAAACTCCCCGCGCGCAATGGGCACACTATGTGCAATCCGTCATGCAGCAATTCGATTTAATTCAACTTGCCGATGAATACCCTTCCGCGCTGCCTCTTGGCATTCGGCAACGTTTGCAGCTTGCCGCCGCCTGTTTGCATAAAc                                      >  1:538059/1‑250 (MQ=255)                                                                                                                                                                                                           gCAATATCGACACGCGAAAACGGGTGGGTTATATGTCCCAAGCGTTTTCCTTATATGAAGAACTCAGCGTTTATCAAAATCTGGAATTGCACGCCAAGCTCTATCAAACTCCCCGCGCGCAATGGGCACACTATGTGCAATCCGTCATGCAGCAATTCGATTTAATTCAACTTGCCGATGAATACCCTTCCGCGCTGCCTCTTGGCATTCGGCAACGTTTGCAGCTTGCCGCCGCCTGTTTGCATAAAcc                                     <  1:262734/250‑1 (MQ=255)                                                                                                                                                                                                              atatCGACACGCGAAAACGGGTGGGTTATATGTCCCAAGCGTTTTCCTTATATGAAGAACTCAGCGTTTATCAAAATCTGGAATTGCACGCCAAGCTCTATCAAACTCCCCGCGCGCAATGGGCACACTATGTGCAATCCGTCATGCAGCAATTCGATTTAATTCAACTTGCCGATGAATACCCTTCCGCGCTGCCTCTTGGCATTCGGCAACGTTTGCAGCTTGCCGCCGCCTGTTTGCATAAACCCGaa                                 >  1:818331/1‑251 (MQ=255)                                                                                                                                                                                                                         cgAAAACGGGTGGGTTATATGTCCCAAGCGTTTTCCTTATATGAAGAACTCAGCGTTTATc                                                                                                                                                                                                                    >  1:45200/1‑61 (MQ=255)                                                                                                                                                                                                                         cgAAAACGGGTGGGTTATATGTCCCAAGCGTTTTCCTTATATGAAGAACTCAGCGTTTATCAAAATCTGGAATTGCACGCCAAGCTCTATCAAACTCCCCGCGCGCAATGGGCACACTATGTGCAATCCGTCATGCAGCAATTCGATTTAATTCAACTTGCCGATGAATACCCTTCCGCGCTGCCTCTTGGCATTCGGCAACGTTTGCAGCTTGCCGCCGCCTGTTTGCATAAACCCGAAGTGCTGATTCt                      <  1:78200/251‑1 (MQ=255)                                                                                                                                                                                                                              aCGGGTGGGTTATATGTCCCAAGCGTTTTCCTTATATGAAGAACTCAGCGTTTATCAAAATCTGGAATTGCACGCCAAGCTCTATCAAACTCCCCGCGCGCAATGGGCACACTATGTGCAATCCGTCATGCAGCAATTCGATTTAATTCAACTTGCCGATGAATACCCTTCCGCGCTGCCTCTTGGCATTCGGCAACGTTTGCAGCTTGCCGCCGCCTGTTTGCATAAACCCGAAGTGCTGATTCTGGACg                 <  1:858475/251‑1 (MQ=255)                                                                                                                                                                                                                                    gggtTATATGTCCCAAGCGTTTTCCTTATATGAAGAACTCAGCGTTTATCAAAATCTGGAATTGCACGCCAAGCTCTATCAAACTCCCCGCGCGCAATGGGCACACTATGTGCAATCCGTCATGCAGCAATTc                                                                                                                                 <  1:52590/133‑1 (MQ=255)                                                                                                                                                                                                                                    gggtTATATGTCCCAAGCGTTTTCCTTATATGAAGAACTCAGCGTTTATCAAAATCTGGAATTGCACGCCAAGCTCTATCAAACTCCCCGCGCGCAATGGGCACACTATGTGCAATCCGTCATGCAGCAATTCGATTTAATTCAACTTGCCGATGAATACCCTTCCGCGCTGCCTCTTGGCATTCGGCAACGTTTGCAGc                                                              <  1:901898/200‑1 (MQ=255)                                                                                                                                                                                                                                    gggtTATATGTCCCAAGCGTTTTCCTTATATGAAGAACTCAGCGTTTATCAAAATCTGGAATTGCACGCCAAGCTCTATCAAACTCCCCGCGCGCAATGGGCACACTATGTGCAATCCGTCATGCAGCAATTCGATTTAATTCAACTTGCCGATGAATACCCTTCCGCGCTGCCTCTTGGCATTCGGCAACGTTTGCAGCTTGCCGCCGCCTGTTTGCATAAAcc                                     <  1:697798/225‑1 (MQ=255)                                                                                                                                                                                                                                    gggtTATATGTCCCAAGCGTTTTCCTTATATGAAGAACTCAGCGTTTATCAAAATCTGGAATTGCACGCCAAGCTCTATCAAACTCCCCGCGCGCAATGGGCACACTATGTGCAATCCGTCATGCAGCAATTCGATTTAATTCAACTTGCCGATGAATACCCTTCCGCGCTGCCTCTTGGCATTCGGCAACGTTTGCAGCTTGCCGCCGCCTGTTTGCATAAACCCGAAGTGCTGATTCTGGACGAACCTa           >  1:701062/1‑251 (MQ=255)                                                                                                                                                                                                                                    gggtTATATGTCCCAAGCGTTTTCCTTATATGAAGAACTCAGCGTTTATCAAAATCTGGAATTGCACGCCAAGCTCTATCAAACTCCCCGCGCGCAATGGGCACACTATGTGCAATCCGTCATGCAGCAATTCGATTTAATTCAACTTGCCGATGAATACCCTTCCGCGCTGCCTCTTGGCATTCGGCAACGTTTGCAGCTTGCCGCCGCCTGTTTGCATAAACCCGAAGTGCTGATTCTGGACGAACCTa           >  1:259988/1‑251 (MQ=255)                                                                                                                                                                                                                                     ggtTATATGTCCCAAGCGTTTTCCTTATATGAAGAACTCAGCGTTTATCAAAATCTGGAATTGCACGCCAAGCTCTATCAAACTCCCCGCGCGCAATGGGCACACTATGTGCAATCCGTCATGCAGCAATTCGATTTAATTCAACTTGCCGATGAATACCCTTc                                                                                                 >  1:268368/1‑164 (MQ=255)                                                                                                                                                                                                                                     ggtTATATGTCCCAAGCGTTTTCCTTATATGAAGAACTCAGCGTTTATCAAAATCTGGAATTGCACGCCAAGCTCTATCAAACTCCCCGCGCGCAATGGGCACACTATGTGCAATCCGTCATGCAGCAATTCGATTTAATTCAACTTGCCGATGAATACCCTTCCGCGCTGCCTCTTGGc                                                                                 >  1:420187/1‑180 (MQ=255)                                                                                                                                                                                                                                     ggtTATATGTCCCAAGCGTTTTCCTTATATGAAGAACTCAGCGTTTATCAAAATCTGGAATTGCACGCCAAGCTCTATCAAACTCCCCGCGCGCAATGGGCACACTATGTGCAATCCGTCATGCAGCAATTCGATTTAATTCAACTTGCCGATGAATACCCTTCCGCGCTGCCTCTTGGCATTCGGCAACGTTTGCAGCTTGCCGCCGCCTGTTTGCATAAACCCGAAGTGCTGATTCTGGACGAACCTa           >  1:513508/1‑250 (MQ=255)                                                                                                                                                                                                                                      gtTATATGTCCCAAGCGTTTTCCTTATATGAAGAACTCAGCGTTTATCAAAATCTGGAATTGCACGCCAAGCTCTATCAAACTCCCCGCGCGCAATGGGCACACTATGTGCAATCCGTCATGCAGCAATTCGATTTAATTCAACTTGCCGATGAATACCCTTCCGCGCTGCCTCTTGGCATTCGGCAACGTTTGCAGCTTGCCGCCGCCTGTTTGCATAAACCCGAAGTGCTGATTCTGGACGAACCTa           >  1:94537/1‑249 (MQ=255)                                                                                                                                                                                                                                      gtTATATGTCCCAAGCGTTTTCCTTATATGAAGAACTCAGCGTTTATCAAAATCTGGAATTGCACGCCAAGCTCTATCAAACTCCCCGCGCGCAATGGGCACACTATGTGCAATCCATCATGCAGCAATTCGATTTAATTCAACTTGCCGATGAATACCCTTCCGCGCTGCCTCTTGGCATTCGGCAACGTTTGCAGCTTGCCGCCGCCTGTTTGCATAAACCCGAAGTGCTGATTCTGGACGAACCTAc          <  1:318427/250‑1 (MQ=255)                                                                                                                                                                                                                                        tataTGTCCCAAGCGTTTTCCTTATATGAAGAACTCAGCGTTTATCAAAATCTGGAATTGCACGCCAAGCTCTATCAAACTCCCCGCGCGCAATGGGCACACTATGTGCAATCCGTCATGCAGCAATTCGATTTAATTCAACTTGCCGATGAATACCCTTCCGCGCTGCCTCTTGGCATTCGGCAACGTTTGCAGCTTGCCGCCGCCTGTTTGCATAAACCCGAAGTGCTGATTCTGGACGAACCTACTTc       <  1:823253/251‑1 (MQ=255)                                                                                                                                                                                                                                         atatGTCCCAAGCGTTTTCCTTATATGAAGAACTCAGCGTTTATCAAAATCTGGAATTGCACGCCAAGCTCTATCAAACTCCCCGCGCGCAATGGGCACACTATGTGCAATCCGTCATGCAGCAATTCGATTTAATTCAACTTGCCGATGAATACCCTTCCGCGCTGCCTCTTGGCATTCGGCAACGTTTGCAGCTTGCCGCCGCCTGTTTGCATAAACCCGAAGTGCTGATTCTGGACGAACCTACTTCa      >  1:465223/1‑251 (MQ=255)                                                                                                                                                                                                                                         atatGTCCCAAGCGTTTTCCTTATATGAAGAACTCAGCGTTTATCAAAATCTGGAATTGCACGCCAAGCTCTA‑CAAACTCCTCGCGCGCAATGGGCACACTATGTGCAATCCGTCATGCAGCAATTCGATTTAATTCAACTTGCCGATGAATACCCTTCCGCGCTGCCTCTTGGCATTCGGCAACGTTTGCAGCTTGCCGCCGCCTGTTTGCATAAACCCGAAGTGCTGATTCTGGACGAACCTACTTCAg     <  1:423580/251‑1 (MQ=255)                                                                                                                                                                                                                                             gTCCCAAGCGTTTTCCTTATATGAAGAACTCAGCGTTTATCAAAATCTGGAATTGCACGCCAAGCTCTATCAAACTCCCCGCGCGCAATGGGCACACTATGTGCAATCCGTCATGCAGCAATTCGATTTAATTCAACTTGCCGATGAATACCCTTCCGCGCTGCCTCTTGGCATTCGGCAACGTTTGCAGCTTGCCGCCGCCTGTTTGCATAAACCCGAAGTGCTGATTCTGGACGAACCTACTTCAgggg  >  1:681005/1‑251 (MQ=255)                                                                                                                                                                                                                                              tCCCAAGCGTTTTCCTTATATGAAGAACTCAGCGTTTATCAAAATCTGGAATTGCACGCCAAGCTCTATCAAACTCCCCGCGCGCAATGGGCACACTATGTGCAATCCGTCATGCAGCAATTCGATTTAATTCAACTTGCCGATGAATACCCTTCCGCGCTGCCTCTTGGCATTCGGCAACGTTTGCAGCTTGCCGCCGCCTGTTTGCATAAACCCGAAg                                <  1:467171/220‑1 (MQ=255)                                                                                                                                                                                                                                               cccAAGCGTTTTCCTTATATGAAGAACTCAGCGTTTATCAAAATCTGGAATTGCACGCCAAGCTCTATCAAACTCCCCGCGCGCAATGGGCACACTATGTGCAATCCGTCATGCAGCAATTCGATTTAATTCAACTTGCCGATGAATACCCTTCCGCGCTGCCTCTTGGCATTCGGCAACg                                                                      >  1:238412/1‑181 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                  GTTATCGTTGCCAAAGGTTTAACCAAGAAATTCGGTGATTTCGTTTCGGTGGATAACGTCAGTTTTACCATTCCGAAAGGAGAAATTTTCGGCTTTTTAGGCTCCAACGGTTGCGGCAAATCCACCACCATGAAAATGCTTACCGGCTTGCTGGATCCGACGGAAGGCTCTGCCACGCTGCTCGGTCAGCCTATTGATGCGGGCAATATCGACACGCGAAAACGGGTGGGTTATATGTCCCAAGCATTTTCCTTATATGAAGAACTCAGCGTTTATCAAAATCTGGAATTGCACGCCAAGCTCTATCAAACTCCCCGCGCGCAATGGGCACACTATGTGCAATCCGTCATGCAGCAATTCGATTTAATTCAACTTGCCGATGAATACCCTTCCGCGCTGCCTCTTGGCATTCGGCAACGTTTGCAGCTTGCCGCCGCCTGTTTGCATAAACCCGAAGTGCTGATTCTGGACGAACCTACTTCAGGGG  >  1/1498134‑1498620

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑