Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,464,084	T→G	P816P (CCT→CCG)	unknown →	Glycogen phosphorylase (EC 2.4.1.1)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,464,084	0	T	G	100.0%	225.0 / NA	67	P816P (CCT→CCG)	unknown	Glycogen phosphorylase (EC 2.4.1.1)
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (40/27);  total (40/27)

TTCTCACCGAATGACCCGAATCGCTATCTGCAATTGTTACAGGGTCTCCAATATCACGACTATTATCAGGCATTCGCCGATTTCCGCAGTTATGTGGATATGCAAAAAGTGGTGGACGAGAAGTATAAAAATCGAGATGCGTGGATTGAAAGCACCATTCAAAACATCGTCAACATGGGCTATTTCTCCTCGGACCGGACGATTAAGGAATATGCGGAAAATATCTGGAATATTGAACCTCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCATATTTGAGCGGGGTTTTGTTTATTTATGAAAAGTGCGGTTGAAATTTTCAGTGTTTTTGAAGTGAAAAGTGCATCAAAGACGACCGCACTTTTAGTTGGCGGGTTTTGTTTTGCGTGCTTTATACCATTTCCAGGCAACGAATATGACTAAAACGGCAACCAGAATATAAATCACCGACTGTCCTTTTTTTATTT  >  1/1463845‑1464324                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                 ttCTCACCGAATGACCCGAATCGCTATCTGCAATTGTTACAGGGTCTCCAATATCACGACTATTATCAGGCATTCGCCGATTTCCGCAGTTATGTGGATATGCAAAAAGTGGTGGACGAGAAGTATAAAAATCGAGATGCGTGGATTGAAAGCACCATTCAAAACATCGTCAACATGGGCTATTTCTCCTCGGACCGGACGATTAAGGAATATGCGGAAAATATCTGGAATATTGAACCGCTAAAAc                                                                                                                                                                                                                                           <  1:94694/247‑1 (MQ=255)                                    gTTACAGGGTCTCCAATATCACGACTATTATCAGGCATTCGCCGATTTCCGCAGTTATGTGGATATGCAAAAAGTGGTGGACGAGAAGTATAAAAATCGAGATGCGTGGATTGAAAGCACCATTCAAAACATCGTCAACATGGGCTATTTCTCCTCGGACCGGACGATTAAGGAATATGCGGAAAATATCTGGAATATTGAACCGCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCat                                                                                                                                                                                                    >  1:557317/1‑251 (MQ=255)                                    gTTACAGGGTCTCCAATATCACGACTATTATCAGGCATTCGCCGATTTCCGCAGTTATGTGGATATGCAAAAAGTGGTGGACGAGAAGTATAAAAATCGAGATGCGTGGATTGAAAGCACCATTCAAAACATCGTCAACATGGGCTATTTCCCCTCGGACCGGACGATTAAGGAATATGCGGAAAATATCTGGAATATTGAACCGCTAAAAcc                                                                                                                                                                                                                                          >  1:504149/1‑213 (MQ=255)                                              ctcCAATATCACGACTATTATCAGGCATTCGCCGATTTCCGCAGTTATGTGGATATGCAAAAAGTGGTGGACGAGAAGTATAAAAATCGAGATGCGTGGATTGAAAGCACCATTCAAAACATCGTCAACATGGGCTATTTCTCCTCGGACCGGACGATTAAGGAATATGCGGAAAATATCTGGAATATTGAACCGCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCATATTTGAGCgg                                                                                                                                                                                          >  1:454014/1‑251 (MQ=255)                                                ccAACATCACGACTATTATCAGGCATTCGCCGATTTCCGCAGTTATGTGGATATGCAAAAAGTGGTGGACGAGAAGTATAAAAATCGAGATGCGTGGATTGAAAGCACCATTCAAAACATCGTCAACATGGGCTATTTCTCCTCGGACCGGACGATTAAGGAATATGCGGAAAATATCTGGAATATTGAACCGCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCATATTTGAGCgggg                                                                                                                                                                                        >  1:676647/1‑251 (MQ=255)                                                         cGACTATTATCAGGCATTCGCCGATTTCCGCAGTTATGTGGATATGCAAAAAGTGGTGGACGAGAAGTATAAAAATCGAGATGCGTGGATTGAAAGCACCATTCAAAACATCGTCAACATGGGCTATTTCTCCTCGGACCGGACGATTAAGGAATATGCGGAAAATATCTGGAATATTGAACCGCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCATATTTGAGCGGGGTTTTGttta                                                                                                                                                                               >  1:263955/1‑251 (MQ=255)                                                           aCTATTATCAGGCATTCGCCGATTTCCGCAGTTATGTGGATATGCAAAAAGTGGTGGACGAGAAGTATAAAAATCGAGATGCGTGGATTGAAAGCACCATTCAAAACATCGTCAACATGGGCTATTTCTCCTCGGACCGGACGATTAAGGAATATGCGGAAAATATCTGGAATATTGAACCGCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCATATTTg                                                                                                                                                                                               >  1:868172/1‑233 (MQ=255)                                                              attatCAGGCATTCGCCGATTTCCGCAGTTATGTGGATATGCAAAAAGTGGTGGACGAGAAGTATAAAAATCGAGATGCGTGGATTGAAAGCACCATTCAAAACATCGTCAACATGGGCTATTTCTCCTCGGACCGGACGATTAAGGAATGTGCGGAAAATATCTGGAATATTGAACCGCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCATATTTGAGCGGGGTTTTGTttatttat                                                                                                                                                                          >  1:91690/1‑251 (MQ=255)                                                              attatCAGGCATTCGCCGATTTCCGCAGTTATGTGGATATGCAAAAAGTGGTGGACGAGAAGTATAAAAATCGAGATGCGTGGATTGAAAGCACCATTCAAAACATCGTCAACATGGGCTATTTCTCCTCGGACCGGACGATTAAGGAATATGCGGAAAATATCTGGAATATTGAACCGCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCATATTTGAGCGGGGTTTTGTttatttat                                                                                                                                                                          >  1:212534/1‑251 (MQ=255)                                                              attatCAGGCATTCGCCGATTTCCGCAGTTATGTGGATATGCAAAAAGTGGTGGACGAGAAGTATAAAAATCGAGATGCGTGGATTGAAAGCACCATTCAAAACATCGTCAACATGGGCTATTTCTCCTCGGACCGGACGATTAAGGAATATGCGGAAAATATCTGGAATATTGAACCGCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCATATTTGAGCGGGGTTTTGTttatttat                                                                                                                                                                          >  1:683405/1‑251 (MQ=255)                                                               ttatCAGGCATTCGCCGATTTCCGCAGTTATGTGGATATGCAAAAAGTGGTGGACGAGAAGTATAAAAATCGAGATGCGTGGATTGAAAGCACCATTCAAAACATCGTCAACATGGGCTATTTCTCCTCGGACCGGACGATTAAGGAATATGCGGAAAATATCTGGAATATTGAACCGCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCATAtt                                                                                                                                                                                                 <  1:793297/227‑1 (MQ=255)                                                               ttatCAGGCATTCGCCGATTTCCGCAGTTATGTGGATATGCAAAAAGTGGTGGACGAGAAGTATAAAAATCGAGATGCGTGGATTGAAAGCACCATTCAAAACATCGTCAACATGGGCTATTTCTCCTCGGACCGGACGATTAAGGAATATGCGGAAAATATCTGGAATATTGAACCGCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCATATTTGAGCGGGGTTTTGt                                                                                                                                                                                  <  1:737622/242‑1 (MQ=255)                                                                 atCAGGCATTCGCCGATTTCCGCAGTTATGCGGATATGCAAAAAGCGGTGGACGAGAAGTATAAAAATCGAGATGCGTGGATTGAAAGCACCATTCAAAACATCGTCAACATGGGCTATTTCTCCTCGGACCGGACGATTAAGGAATATGCGGAAAATATCTGGAATATTGAACCGCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCATATTTGAGCGGGGTTTTGTTTATTTATGaa                                                                                                                                                                       >  1:425286/1‑251 (MQ=255)                                                                                aTTTCCGCAGTTATGTGGATATGCAAAAAGTGGTGGACGAGAAGTATAAAAATCGAGATGCGTGGATTGAAAGCACCATTCAAAACATCGTCAACATGGGCTATTTCTCCTCGGACCGGACGATTAAGGAATATGCGGAAAATATCTGGAATATTGAACCGCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCATATTTGAGCGGGGTTTTGTTTATTTATGAAAAGTGCGGTTGAAAt                                                                                                                                                        <  1:176769/251‑1 (MQ=255)                                                                                    ccGCAGTTATGTGGATATGCAAAAAGTGGTGGACGAGAAGTATAAAAATCGAGATGCGTGGATTGAAAGCACCATTCAAAACATCGTCAACATGGGCTATTTCTCCTCGGACCGGACGATTAAGGAATATGCGGAAAATATCTGGAATATTGAACCGCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCATATTTGAGCGGGGTTTTGttt                                                                                                                                                                                <  1:387533/223‑1 (MQ=255)                                                                                       cAGTTATGTGGATATGCAAAAAGTGGTGGACGAGAAGTATAAAAATCGAGATGCGTGGATTGAAAGCACCATTCAAAACATCGTCAACATGGGCTATTTCTCCTCGGACCGGACGATTAAGGAATATGCGGAAAATATCTGGAATATTGAACCGCTAAAAc                                                                                                                                                                                                                                           <  1:730995/161‑1 (MQ=255)                                                                                       cAGTTATGTGGATATGCAAAAAGTGGTGGACGAGAAGTATAAAAATCGAGATGCGTGGATTGAAAGCACCATTCAAAACATCGTCAACATGGGCTATTTCTCCTCGGACCGGACGATTAAGGAATATGCGGAAAATATCTGGAATATTGAACCGCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCATATTTGAGCGGGGTTTTGTTTATTTATGAAAAGTGCGGTTGAAATTTTCAgt                                                                                                                                                 >  1:721819/1‑251 (MQ=255)                                                                                         gTTATGTGGATATGCAAAAAGTGGTGGACGAGAAGTATAAAAATCGAGATGCGTGGATTGAAAGCACCATTCAAAACATCGTCAACATGGGCTATTTCTCCTCGGACCGGACGATTAAGGAATATGCGGAAAATATCTGGAATATTGAACCGCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCATAtt                                                                                                                                                                                                 >  1:519518/1‑201 (MQ=255)                                                                                         gTTATGTGGATATGCAAAAAGTGGTGGACGAGAAGTATAAAAATCGAGATGCGTGGATTGAAAGCACCATTCAAAACATCGTCAACATGGGCTATTTCTCCTCGGACCGGACGATTAAGGAATATGCGGAAAATATCTGGAATATTGAACCGCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCATATTTGAGCGGGGTTTTGtttattta                                                                                                                                                                           <  1:473906/223‑1 (MQ=255)                                                                                         gTTATGTGGATATGCAAAAAGTGGTGGACGAGAAGTATAAAAATCGAGATGCGTGGATTGAAAGCACCATTCAAAACATCGTCAACATGGGCTATTTCTCCTCGGACCGGACGATTAAGGAATATGCGGAAAATATCTGGAATATTGAACCGCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCATATTTGAGCGGGGTTTTGtttattta                                                                                                                                                                           <  1:835485/223‑1 (MQ=255)                                                                                         gTTATGTGGATATGCAAAAAGTGGTGGACGAGAAGTATAAAAATCGAGATGCGTGGATTGAAAGCACCATTCAAAACATCGTCAACATGGGCTATTTCTCCTCGGACCGGACGATTAAGGAATATGCGGAAAATATCTGGAATATTGAACCGCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCATATTTGAGCGGGGTTTTGTTTATTTATGAAAAGTGCGGTTGAAATTTTCAgtgt                                                                                                                                               >  1:762254/1‑251 (MQ=255)                                                                                          ttATGTGGATATGCAAAAAGTGGTGGACGAGAAGTATAAAAATCGAGATGCGTGGATTGAAAGCACCATTCAAAACATCGTCAACATGGGCTATTTCTCCTCGGACCGGACGATTAAGGAATATGCGGAAAATATCTGGAATATTGAACCGCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCATATTTGAGCGGGGTTTTGttta                                                                                                                                                                               <  1:774224/218‑1 (MQ=255)                                                                                           tATGTGGATATGCAAAAAGTGGTGGACGAGAAGTATAAAAATCGAGATGCGTGGATTGAAAGCACCATTCAAAACATCGTCAACATGGGCTATTTCTCCTCGGACCGGACGATTAAGGAATATGCGGAAAATATCTGGAATATTGAACCGCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCATATTTGAGCGGGGTTTTGTTTATTTATGaaa                                                                                                                                                                      >  1:468482/1‑226 (MQ=255)                                                                                                 gATATGCAAAAAGTGGTGGACGAGAAGTATAAAAATCGAGATGCGTGGATTGAAAGCACCATTCAAAACATCGTCAACATGGGCTATTTCTCCTCGGACCGGACGATTAAGGAATATGCGGAAAATATCTGGAATATTGAACCGCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCATATTTGAGCGGGGTTTTGTTTATTTATGAAAAGTGCGGTTGAAATTTTCAGTGTTTTTGAAg                                                                                                                                       >  1:435020/1‑251 (MQ=255)                                                                                                  atatGCAAAAAGTGGTGGACGAGAAGTATAAAAATCGAGATGCGTGGATTGAAAGCACCATTCAAAACATCGTCAACATGGGCTATTTCTCCTCGGACCGGACGATTAAGGAATATGCGGAAAATATCTGGAATATTGAACCGCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCATATTTGAGCGGGGTTTTGTTTATTTATGAAAAGTGCGGTTGAAATTTTCAGTGTTTTTGAAg                                                                                                                                       <  1:523238/250‑1 (MQ=255)                                                                                                    atGCAAAAAGTGGTGGACGAGAAGTATAAAAATCGAGATGCGTGGATTGAAAGCACCATTCAAAACATCGTCAACATGGGCTATTTCTCCTCGGACCGGACGATTAAGGAATATGCGGAAAATATCTGGAATATTGAACCGCTAAAACCGGCAAAATAATCGGTCATTAAAAATc                                                                                                                                                                                                                >  1:32333/1‑175 (MQ=255)                                                                                                     tGCAAAAAGTGGTGGACGAGAAGTATAAAAATCGAGATGCGTGGATTGAAAGCACCATTCAAAACATCGTCAACATGGGCTATTTCTCCTCGGACCGGACGATTAAGGAATATGCGGAAAATATCTGGAATATTGAACCGCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCATATTTGAGCGGGGTTTTGtttattta                                                                                                                                                                           >  1:235413/1‑211 (MQ=255)                                                                                                                        gaAGTATAAAAATCGAGATGCGTGGATTGAAAGCACCATTCAAAACATCGTCAACATGGGCTATTTCTCCTCGGACCGGACGATTAAGGAATATGCGGAAAATATCTGGAATATTGAACCGCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCATATTTGAGCGGGGTTTTGTTTATTTATGAAAAGTGCGGTTGAAATTTTCAGTGTTTTTGAAGTGAAAAGTGCATCAAAgacgac                                                                                                                 >  1:384699/1‑250 (MQ=255)                                                                                                                        gaAGTATAAAAATCGAGATGCGTGGATTGAAAGCACCATTCAAAACATCGTCAACATGGGCTATTTCTCCTCGGACCGGACGATTAAGGAATATGCGGAAAATATCTGGAATATTGAACCGCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCATATTTGAGCGGGGTTTTGTTTATTTATGAAAAGTGCGGTTGAAATTTTCAGTGTTTTTGAAGTGAAAAGTGCATCAAAgacgac                                                                                                                 >  1:279613/1‑250 (MQ=255)                                                                                                                                          tGCGTGGATTGAAAGCACCATTCAAAACATCGTCAACATGGGCTATTTCTCCTCGGACCGGACGATTAAGGAATATGCGGAAAATATCTGGAATATTGAACCGCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCATAtt                                                                                                                                                                                                 >  1:713082/1‑152 (MQ=255)                                                                                                                                               ggATTGAAAGCACCATTCAAAACATCGTCAACATGGGCTATTTCTCCTCGGACCGGACGATTAAGGAATATGCGGAAAATATCTGGAATATTGAACCGCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCATATTTGAGCGGGGTTTTGTTTATTTATGAAAAGTGCGGTTGAAATTTTCAGTGTTTTTGAAGTGAAAAGTGCATCAAAGACGACCGCACTTTTAGTTGGCGGgttttg                                                                                         >  1:750052/1‑251 (MQ=255)                                                                                                                                                 aTTGAAAGCACCATTCAAAACATCGTCAACATGGGCTATTTCTCCTCGGACCGGACGATTAAGGAATATGCGGAAAATATCTGGAATATTGAACCGCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCATATTTGAGCGGGGTTTTGTTTA‑TTATGAAAAGTGCGGTTGAAATTTTCAGTGTTTTTGAAGTGAAAAGTGCATCAAAGACGACCGCACTTTTAGTTGGCGGgttttgttt                                                                                      <  1:670301/251‑1 (MQ=255)                                                                                                                                                  ttGAAAGCACCATTCAAAACATCGTCAACATGGGCTATTTCTCCTCGGACCGGACGATTAAGGAATATGCGGAAAATATCTGGAATATTGAACCGCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCATATTTGAGCGGGGTTTTGTTTATTTATGAAAAGTGCGGTTGAAATTTTCAGTGTTTTTGAAGTGAAAAGTGCATCAAAGACGACCGCACTTTTAGTTGGCGGgttttgttt                                                                                      <  1:593306/251‑1 (MQ=255)                                                                                                                                                    gAAAGCACCATTCAAAACATCGTCAACATGGGCTATTTCTCCTCGGACCGGACGATTAAGGAATATGCGGAAAATATCTGGAATATTGAACCGCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCATATTTg                                                                                                                                                                                               <  1:303050/144‑1 (MQ=255)                                                                                                                                                           ccATTCAAAACATCGTCAACATGGGCTATTTCTCCTCGGACCGGACGATTAAGGAATATGCGGAAAATATCTGGAATATTGAACCGCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCATATTTGAGCGGGGTTTTGTTTATTTATGAAAAGTGCGGTTGAAAtt                                                                                                                                                       >  1:863274/1‑177 (MQ=255)                                                                                                                                                             aTTCAAAACATCGTCAACATGGGCTATTTCTCCTCGGACCGGACGATTAAGGAATATGCGGAAAATATCTGGAATATTGAACCGCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCATATTTGAGCGGGGTTTTGTTTATTTATGAAAAGTGCGGTTGAAATTTTCAGTGTTTTTGAAGTGAAAAGTGCATCAAAGACGACCGCACTTTTAGTTGGCGGgttttgt                                                                                        <  1:256778/238‑1 (MQ=255)                                                                                                                                                             aTTCAAAACATCGTCAACATGGGCTATTTCTCCTCGGACCGGACGATTAAGGAATATGCGGAAAATATCTGGAATATTGAACCGCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCATATTTGAGCGGGGTTTTGTTTATTTATGAAAAGTGCGGTTGAAATTTTCAGTGTTTTTGAAGTGAAAAGTGCATCAAAGACGACCGCACTTTTAGTTGGCGGGTTTTGTTTTGCGTGCTTta                                                                           >  1:553893/1‑251 (MQ=255)                                                                                                                                                             aTTCAAAACATCGTCAACATGGGCTATTTCTCCTCGGACCGGACGATTAAGGAATATGCGGAAAATATCTGGAATATTGAACCGCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCATATTTGAGCGGGGTTTTGTTTATTTATGAAAAGTGCGGTTGAAATTTTCAGTGTTTTTGAAGTGAAAAGTGCATCAAAGACGACCGCACTTTTAGTTGGCGGGTTTTGTTTTGCGTGCTTta                                                                           >  1:724147/1‑251 (MQ=255)                                                                                                                                                                cAAAACATCGTCAACATGGGCTATTTCTCCTCGGACCGGACGATTAAGGAATATGCGGAAAATATCTGGAATATTGAACCGCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCATATTTGAGCGGGGTTTTGTTTATTTATGAAAAGTGCGGTTGAAATTTTCAGTGTTTTTGAAGTGAAAAGTGCATCAAAGACGACCGCACTTTTAGTTGGCGGGTTTTGTTTTGCGTGCTTTATAc                                                                        <  1:110626/251‑1 (MQ=255)                                                                                                                                                                cAAAACATCGTCAACATGGGCTATTTCTCCTCGGACCGGACGATTAAGGAATATGCGGAAAATATCTGGAATATTGAACCGCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCATATTTGAGCGGGGTTTTGTTTATTTATGAAAAGTGCGGTTGAAATTTTCAGTGTTTTTGAAGTGAAAAGTGCATCAAAGACGACCGCACTTTTAGTTGGCGGGTTTTGTTTTGCGTGCTTTATAc                                                                        <  1:728144/251‑1 (MQ=255)                                                                                                                                                                 aaaaCATCGTCAACATGGGCTATTTCTCCTCGGACCGGACGATTAAGGAATATGCGGAAAATATCTGGAATATTGAACCGCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCATATTTGAGCGGGGTTTTGTTTATTTATGAAAAGTGCGGTTGAAATTTTCAGTGTTTTTGAAGTGAAAAGTGCATCAAAGACGACCGCACTTTTAGTTGGCGGGTTTTGTTTTGCGTGCTTTATAcc                                                                       <  1:489032/251‑1 (MQ=255)                                                                                                                                                                 aaaaCATCGTCAACATGGGCTATTTCTCCTCGGACCGGACGATTAAGGAATATGCGGAAAATATCTGGAATATTGAACCGCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCATATTTGAGCGGGGTTTTGTTTATTTATGAAAAGTGCGGTTGAAATTTTCAGTGTTTTTGAAGTGAAAAGTGCATCAAAGACGACCGCACTTTTAGTTGGCGGGTTTTGTTTTGCGTGCTTTATAcc                                                                       <  1:272814/251‑1 (MQ=255)                                                                                                                                                                      aTCGTCAACATGGGCTATTTCTCCTCGGACCGGACGATTAAGGAATATGCGGAAAATATCTGGAATATTGAACCGCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCATATTTGAGCGGGGTTTTGTTTATTTATGAAAAGTGCGGTTGAAATTTTCAGTGTTTTTGAAGTGAAAAGTGCATCAAAGACGACCGCACTTTTAGTTGGCGGGTTTTGTTTTGCGTGCTTTATAcc                                                                       <  1:794927/246‑1 (MQ=255)                                                                                                                                                                           cAACATGGGCTATTTCTCCTCGGACCGGACGATTAAGGAATATGCGGAAAATATCTGGAATATTGAACCGCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCATATTTGAGCGGGGTTTTGTTTATTTATGAAAAGTGCGGTTGAAATTTTCAGTGTTTTTGAAGTGAAAAGTGCATCAAAGACGACCGCACTTTTAGTTGGCGGGTTTTGTTTTGCGTGCTTTATACCATTTCCAGGc                                                             <  1:326026/251‑1 (MQ=255)                                                                                                                                                                                  ggCTATTTCTCCTCGGACCGGACGATTAAGGAATATGCGGAAAATATCTGGAATATTGAACCGCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCATATTTGAGCGGGGTTTTGTTTATTTATGAAAAGTGCGGTTGAAATTTTCAGTGTTTTTGAAGTGAAAAGTGCATCAAAGACGACCGCACTTTTAGTTGGCGGgttttgttt                                                                                      <  1:599083/219‑1 (MQ=255)                                                                                                                                                                                  ggCTATTTCTCCTCGGACCGGACGATTAAGGAATATGCGGAAAATATCTGGAATATTGAACCGCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCATATTTGAGCGGGGTTTTGTTTATTTATGAAAAGTGCGGTTGAAATTTTCAGTGTTTTTGAAGTGAAAAGTGCATCAAAGACGACCGCACTTTTAGTTGGCGGGTTTTGTTTTGCGTGCTTTATACCATTTCCAGGCAACGAat                                                      >  1:743450/1‑251 (MQ=255)                                                                                                                                                                                    cTATTTCTCCTCGGACCGGACGATTAAGGAATATGCGGAAAATATCTGGAATATTGAACCGCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCATATTTGAGCGGGGTTTTGTTTATTTATGAAAAGTGCGGTTGAAATTTTCAGTGTTTTTGAAGTGAAAAGTGCATCAAAGACGACCGCACTTTTAGTTGGCGGGTTTTGTTTTGCGTGCTTTATACCATTTCCAGGCAACGAatat                                                    >  1:381750/1‑251 (MQ=255)                                                                                                                                                                                      aTTTCTCCTCGGACCGGACGATTAAGGAATATGCGGAAAATATCTGGAATATTGAACCGCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCATATTTGAGCGGGGTTTTGTTTATTTATGAAAAGTGCGGTTGAAATTTTCAGTGTTTTTGAAGTGAAAAGTGCATCAAAGACGACCGCACTTTTAGTTGGCGGGTTTTGTTTTGCGTGCTTTATAcc                                                                       <  1:389219/230‑1 (MQ=255)                                                                                                                                                                                       tttCTCCTCGGACCGGACGATTAAGGAATATGCGGAAAATATCTGGAATATTGAACCGCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCATATTTGAGCGGGGTTTTGTTTATTTATGAAAAGTGCGGTTGAAATTTTCAGTGTTTTTGAAGTGAAAAGTGCATCAAAGACGACCGCACTTTTAGTTGGCGGGTTTTGTTTTGCGTGCTTTATACCATTTc                                                                  >  1:78189/1‑234 (MQ=255)                                                                                                                                                                                       tttCTCCTCGGACCGGACGATTAAGGAATATGCGGAAAATATCTGGAATATTGAACCGCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCATATTTGAGCGGGGTTTTGTTTATTTATGAAAAGTGCGGTTGAAATTTTCAGTGTTTTTGAAGTGAAAAGTGCATCAAAGACGACCGCACTTTTAGTTGGCGGGTTTTGTTTTGCGTGCTTTATACCATTTCCAGGCAACGAATATGa                                                  >  1:835146/1‑250 (MQ=255)                                                                                                                                                                                                        cGATTAAGGAATATGCGGAAAATATCTGGAATATTGAACCGCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCATATTTGAGCGGGGTTTTGTTTATTTATGAAAAGTGCGGTTGAAATTTTCAGTGTTTTTGAAGTGAAAAGTGCATCAAAGACGACCGCACTTTTAGTTGGCGGGTTTTGTTTTGCGTGCTTTATACCATTTCCAGGCAACGAATATGACTAAAACGGCAACCAGaa                                >  1:224080/1‑251 (MQ=255)                                                                                                                                                                                                         gATTAAGGAATATGCGGAAAATATCTGGAATATTGAACCGCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCATATTTGAGCGGGGTTTTGTTTATTTATGAAAAGTGCGGTTGAAATTTTCAGTGTTTTTGAAGTGAAAAGTGCATCAAAGACGACCGCACTTTTAGTTGGCGGGTTTTGTTTTGCGTGCTTTATACCATTTCCAGGCAACGAATATGACTAAAACGGCAACCAGAat                               >  1:29129/1‑251 (MQ=255)                                                                                                                                                                                                          aTTAAGGAATATGCGGAAAATATCTGGAATATTGAACCGCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCATATTTGAGCGGGGTTTTGTTTATTTATGAAAAGTGCGGTTGAAATTTTCAGTGTTTTTGAAGTGAAAAGTGCATCAAAGACGACCGCACTTTTAg                                                                                                      >  1:666730/1‑179 (MQ=255)                                                                                                                                                                                                          aTTAAGGAATATGCGGAAAATATCTGGAATATTGAACCGCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCATATTTGAGCGGGGTTTTGTTTATTTATGAAAAGTGCGGTTGAAATTTTCAGTGTTTTTGAAGTGAAAAGTGCATCAAAGACGACCGCACTTTTAGTTGGCGGGTTTTGTTTTGCGTGCTTTATACCATTTc                                                                  <  1:767085/215‑1 (MQ=255)                                                                                                                                                                                                          aTTAAGGAATATGCGGAAAATATCTGGAATATTGAACCGCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCATATTTGAGCGGGGTTTTGTTTATTTATGAAAAGTGCGGTTGAAATTTTCAGTGTTTTTGAAGTGAAAAGTGCATCAAAGACGACCGCACTTTTAGTTGGCGGGTTTTGTTTTGCGTGCTTTATACCATTTCCAGGCAACGAATATGACTAAAAc                                           <  1:452786/238‑1 (MQ=255)                                                                                                                                                                                                          aTTAAGGAATATGCGGAAAATATCTGGAATATTGAACCGCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCATATTTGAGCGGGGTTTTGTTTATTTATGAAAAGTGCGGTTGAAATTTTCAGTGTTTTTGAAGTGAAAAGTGCATCAAAGACGACCGCACTTTTAGTTGGCGGGTTTTGTTTTGCGTGCTTTATACCATTTCCAGGCAACGAATATGACTAAAACGGCAACCAGAata                              >  1:612886/1‑251 (MQ=255)                                                                                                                                                                                                          aTTAAGGAATATGCGGAAAATATCTGGAATATTGAACCGCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCATATTTGAGCGGGGTTTTGTTTATTTATGAAAAGTGCGGTTGAAATTTTCAGTGTTTTTGAAGTGAAAAGTGCATCAAAGACGACCGCACTTTTAGTTGGCGGGTTTTGTTTTGCGTGCTTTATACCATTTCCAGGCAACGAATATGACTAAAACGGCAACCAGAata                              >  1:275692/1‑251 (MQ=255)                                                                                                                                                                                                          aTTAAGGAATATGCGGAAAATATCTGGAATATTGAACCGCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCATATTTGAGCGGGGTTTTGTTTATTTATGAAAAGTGCGGTTGAAATTTTCAGTGTTTTTGAAGTGAAAAGTGCATCAAAGACGACCGCACTTTTAGTTGGCGGGTTTTGTTTTGCGTGCTTTATACCATTTCCAGGCAACGAATATGACTAAAACGGCAACCAGAata                              >  1:41387/1‑251 (MQ=255)                                                                                                                                                                                                            tAAGGAATATGCGGAAAATATCTGGAATATTGAACCGCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCATATTTGAGCGGGGTTTTGTTTATTTATGAAAAGTGCGGTTGAAATTTTCAGTGTTTTTGAAGTGAAAAGTGCATCAAAGACGACCGCACTTTTAGTTGGCGGGTTTTGTTTTGCGTGCTTTATACCATTTCCAGGCAACGAATATGACTAAAACGGCAACCAGAAtata                            >  1:356972/1‑251 (MQ=255)                                                                                                                                                                                                                gAATATGCGGAAAATATCTGGAATATTGAACCGCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCATATTTGAGCGGGGTTTTGTTTATTTATGAAAAGTGCGGTTGAAATTTTCAGTGTTTTTGAAGTGAAAAGTGCATCAAAGACGACCGCACTTTTAGTTGGCGGGTTTTGTTTTGCGTGCTTTATACCATTTCCAGGCAACGAATATGACTAAAACGGCAACCAGAATATaaa                          <  1:379122/249‑1 (MQ=255)                                                                                                                                                                                                                  atatGCGGAAAATATCTGGAATATTGAACCGCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCATATTTGAGCGGGGTTTTGTTTATTTATGAAAAGTGCGGTTGAAATTTTCAGTGTTTTTGAAGTGAAAAGTGCATCAAAGACGACCGCACTTTTAGTTGGCGGGTTTTGTTTTGCGTGCTTTATACCATTTCCAGGCAACGAATATGACTAAAACGGCAACCAGAATATAAATCAc                      <  1:667065/251‑1 (MQ=255)                                                                                                                                                                                                                               atCTGGAATATTGAACCGCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCATATTTGAGCGGGGTTTTGTTTATTTATGAAAAGTGCGGTTGAAATTTTCAGTGTTTTTGAAGTGAAAAGTGCATCAAAGACGACCGCACTTTTAGTTGGCGGGTTTTGTTTTGCGTGCTTTATACCATTTCCAGGCAACGAATATGACTAAAACGGCAACCAGAATATAAATCACCGACTGTCCtttt         >  1:900540/1‑251 (MQ=255)                                                                                                                                                                                                                                   ggAATATTGAACCGCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCATATTTGAGCGGGGTTTTGTTTATTTATGAAAAGTGCGGTTGAAATTTTCAGTGTTTTTGAAGTGAAAAGTGCATCAAAGACGACCGCACTTTTAGTTGGCGGGTTTTGTTTTGCGTGCTTTATACCATTTCCAGGCAACGAATATGACTAAAACGGCAACCAGAatat                             >  1:117142/1‑227 (MQ=255)                                                                                                                                                                                                                                   ggAATATTGAACCGCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCATATTTGAGCGGGGTTTTGTTTATTTATGAAAAGTGCGGTTGAAATTTTCAGTGTTTTTGAAGTGAAAAGTGCATCAAAGACGACCGCACTTTTAGTTGGCGGGTTTTGTTTTGCGTGCTTTATACCATTTCCAGGCAACGAATATGACTAAAACGGCAACCAGAATATaaa                          <  1:52199/230‑1 (MQ=255)                                                                                                                                                                                                                                      atatTGAACCGCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCATATTTGAGCGGGGTTTTGTTTATTTATGAAAAGTGCGGTTGAAATTTTCAGTGTTTTTGAAGTGAAAAGTGCATCAAAGACGACCGCACTTTTAGTTGGCGGGTTTTGTTTTGCGTGCTTTATACCATTTCCAGGCAACGAATATGACTAAAACGGCAACCAGAATATAAATCACCGACTGTCCTTTTTTTAttt  >  1:761446/1‑251 (MQ=255)                                                                                                                                                                                                                                      atatTGAACCGCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCATATTTGAGCGGGGTTTTGTTTATTTATGAAAAGTGCGGTTGAAATTTTCAGTGTTTTTGAAGTGAAAAGTGCATCAAAGACGACCGCACTTTTAGTTGGCGGGTTTTGTTTTGCGTGCTTTATACCATTTCCAGGCAACGAATATGACTAAAACGGCAACCAGAATATAAATCACCGACTGTCCTTTTTTTAttt  >  1:397986/1‑251 (MQ=255)                                                                                                                                                                                                                                      atatTGAACCGCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCATATTTGAGCGGGGTTTTGTTTATTTATGAAAAGTGCGGTTGAAATTTTCAGTGTTTTTGAAGTGAAAAGTGCATCAAAGACGACCGCACTTTTAGTTGGCGGGTTTTGTTTTGCGTGCTTTATACCATTTCCAGGCAACGAATATGACTAAAACGGCAACCAGAATATAAATCACCGACTGTCCTTTTTTTAttt  >  1:284205/1‑251 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                 TTCTCACCGAATGACCCGAATCGCTATCTGCAATTGTTACAGGGTCTCCAATATCACGACTATTATCAGGCATTCGCCGATTTCCGCAGTTATGTGGATATGCAAAAAGTGGTGGACGAGAAGTATAAAAATCGAGATGCGTGGATTGAAAGCACCATTCAAAACATCGTCAACATGGGCTATTTCTCCTCGGACCGGACGATTAAGGAATATGCGGAAAATATCTGGAATATTGAACCTCTAAAACCGGCAAAATAATCGGTCATTAAAAATCAACCCCGTTCATATTTGAGCGGGGTTTTGTTTATTTATGAAAAGTGCGGTTGAAATTTTCAGTGTTTTTGAAGTGAAAAGTGCATCAAAGACGACCGCACTTTTAGTTGGCGGGTTTTGTTTTGCGTGCTTTATACCATTTCCAGGCAACGAATATGACTAAAACGGCAACCAGAATATAAATCACCGACTGTCCTTTTTTTATTT  >  1/1463845‑1464324

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑