Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	971,582	C→T	A136T (GCC→ACC)	unknown ←	Chaperone protein HscB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	971,582	0	C	T	100.0%	221.4 / NA	66	A136T (GCC→ACC)	unknown	Chaperone protein HscB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (44/22);  total (44/22)

GCGCACGGAGGCAATGAGGGAATTGGTGGTGCCGAGATCAATGCCTACCGCCAGTTTATGTTGATGCGGTGCGGCGCTTTGACCGGGCTCTGCAATTTGAAGTAATGCCATGTTATATCTCGATGTTTTTATTATAAATCAAATAGATTTTCTTCAACCCGTTCAATTTCGGCTTGGAGTTTTTTGATAAAACGTAATTTATCCGTAATCGCCCGCGCCGTTTGCCATTGTTGTTCGTTAAGTGCGGCGGATAATTCCGTTAAAATGGCGCTGCGGGTTTGCTGAATTTCTTGGCTGAAAGCGGTTAATTCGTTGGCATTTTTTTGCTGTTCGATATTTTCCAGGGTTTCCCGCCATTCCATTTGCTGCATCAAAAAGGCAATATCATTGGTGCTTTTTTCTTCTACGTTTTCCGTTTCGCCGGTGTTGATGTCGATAATGCTGTCGGCGCGTGCGATGGGATCTTTTAAAATGCGCAGGGCATCATTAATT  >  1/971335‑971826                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                     gcgcACGGAGGCAATGAGGGAATTGGTGGTGCCGAGATCAATGCCTACCGCCAGTTTATGTTGATGCGGTGCGGCGCTTTGACCGGGCTCTGCAATTTGAAGTAATGCCATGTTATATCTCGATGTTTTTATTATAAATCAAATAGATTTTCTTCAACCCGTTCAATTTCGGCTTGGAGTTTTTTGATAAAACGTAATTTATCCGTAATCGCCCGCGCCGTTTGCCATTGTTGTTCGTTAAGTGCGGTGGa                                                                                                                                                                                                                                                   >  1:809288/1‑251 (MQ=255)     aCGGAGGCAATGAGGGAATTGGTGGTGCCGAGATCAATGCCTACCGCCAGTTTATGTTGATGCGGTGCGGCGCTTTGACCGGGCTCTGCAATTTGAAGTAATGCCATGTTATATCTCGATGTTTTTATTATAAATCAAATAGATTTTCTTCAACCCGTTCAATTTCGGCTTGGAGTTTTTTGATAAAACGTAATTTATCCGTAATCGCCCGCGCCGTTTGCCATTGTTGTTCGTTAAGTGCGGTGGATAAt                                                                                                                                                                                                                                               <  1:107953/251‑1 (MQ=255)     aCGGAGGCAATGAGGGAATTGGTGGTGCCGAGATCAATGCCTACCGCCAGTTTATGTTGATGCGGTGCGGCGCTTTGACCGGGCTCTGCAATTTGAAGTAATGCCATGTTATATCTCGATGTTTTTATTATAAATCAAATAGATTTTCTTCAACCCGTTCAATTTCGGCTTGGAGTTTTTTGATAAAACGTAATTTATCCGTAATCGCCCGCGCCGTTTGCCATTGTTGTTCGTTAAGTGCGGTGGATAAt                                                                                                                                                                                                                                               <  1:204213/251‑1 (MQ=255)        gAGGCAATGAGGGAATTGGTGGTGCCGAGATCAATGCCTACCGCCAGTTTATGTTGATGCGGTGCGGCGCTTTGACCGGGTTCTGCAATTTGAAGTAATGCCATGTTATATCTCGATGTTTTTATTATAAATCAAATAGATTTTCTTCATCCCGTTCAATTTCGGCTTGGAGTTTTTTGATAAAACGTAATTTATCCGTAATCGCCCGCGCCGTTTGCCATTGTTGTTCGTTAAGTGCGGTGGATAATTc                                                                                                                                                                                                                                             <  1:451853/250‑1 (MQ=255)                    gAATTGGTGGTGCCGAGATCAATGCCTACCGCCAGTTTATGTTGATGCGGTGCGGCGCTTTGACCGGGCTCTGCAATTTGAAGTAATGCCATGTTATATCTCGATGTTTTTATTATAAATCAAATAGATTTTCTTCAACCCGTTCAATTTCGGCTTGGAGTTTTTTGATAAAACGTAATTTATCCGTAATCGCCCGCGCCGTTTGCCATTGTTGTTCGTTAAGTGCGGTGGATAATTCCGTTAAAATGgcg                                                                                                                                                                                                                                >  1:674840/1‑251 (MQ=255)                             gtgCCGAGATCAATGCCTACCGCCAGTTTATGTTGATGCGGTGCGGCGCTTTGACCGGGCTCTGCAATTTGAAGTAATGCCATGTTATATCTCGATGTTTTTATTATAAATCAAATAGATTTTCTTCAACCCGTTCAATTTCGGCTTGGAGTTTTTTGATAAAACGTAATTTATCCGTAATCGCCCGCGCCGTTTGCCATTGTTGTTCGTTAAGTGCGGTGGATAATTCCGTTAAAATGGCGCTGCGGGtt                                                                                                                                                                                                                       >  1:325684/1‑251 (MQ=255)                                    gaTCAATGCCTACCGCCAGTTTATGTTGATGCGGTGCGGCGCTTTGACCGGGCTCTGCAATTTGAAGTAATGCCATGTTATATCTCGATGTTTTTATTATAAATCAAATAGATTTTCTTCAACCCGTTCAATTTCGGCTTGGAGTTTTTTGATAAAACGTAATTTATCCGTAATCGCCCGCGCCGTTTGCCATTGTTGTTCGTTAAGTGCGGTGGATAATTCCGTTAAAATGGCGCTGCGGGTTTGCTGaa                                                                                                                                                                                                                >  1:294251/1‑251 (MQ=255)                                                 cGCCAGTTTATGTTGATGCGGTGCGGCGCTTTGACCGGGCTCTGCAATTTGAAGTAATGCCATGTTATATCTCGATGTTTTTATTATAAATCAAATAGATTTTCTTCAACCCGTTCAATTTCGGCTTGGAGTTTTTTGATAAAACGTAATTTATCCGTAATCGCCCGCGCCGTTTGCCATTGTTGTTCGTTAAGTGCGGTGGATAATTCCGTTAAAATGGCGCTGCGGGTTTGCTGAATTTCTTGGCTGaa                                                                                                                                                                                                   >  1:766587/1‑251 (MQ=255)                                                     aGTTTATGTTGATGCGGTGCGGCGCTTTGACCGGGCTCTGCAATTTGAAGTAATGCCATGTTATATCTCGATGTTTTTATTATAAATCAAATAGATTTTCTTCAACCCGTTCAATTTCGGCTTGGAGTTTTTTGATAAAACGTAATTTATCCGTAATCGCCCGCGCCGTTTGCCATTGTTGTTCGTTAAGTGCGGTGGATAATTCCGTTAAAATGGCGCTGCGGGTTTGCTGAATTTCTTGGCTGAAAGCg                                                                                                                                                                                               >  1:543478/1‑251 (MQ=255)                                                      gTTTATGTTGATGCGGTGCGGCGCTTTGACCGGGCTCTGCAATTTGAAGTAATGCCATGTTATATCTCGATGTTTTTATTATAAATCAAATAGATTTTCTTCAACCCGTTCAATTTCGGCTTGGAGTTTTTTGATAAAACGTAATTTATCCGTAATCGCCCGCGCCGTTTGCCATTGTTGTTCGTTAAGTGCGGTGGATAATTCCGTTAAAATGGCGCTGCGGGTTTGCTGAATTTCTTGGCTGAAAGCgg                                                                                                                                                                                              >  1:415256/1‑251 (MQ=255)                                                          aTGTTGATGCGGTGCGGCGCTTTGACCGGGCTCTGCAATTTGAAGTAATGCCATGTTATATCTCGATGTTTTTATTATAAATCAAATAGATTTTCTTCAACCCGTTCAATTTCGGCTTGGAGTTTTTTGATAAAACGTAATTTATCCGTAATCGCCCGCGCCGTTTGCCATTGTTGTTCGTTAAGTGCGGTGGATAATTCCGTTAAAATGGCGCTGCGGGTTTGCTGAATTTCTTGGCTGAAAGCGGTTaa                                                                                                                                                                                          >  1:398209/1‑251 (MQ=255)                                                                                 gACCGGGCTCTGCAATTTGAAGTAATGCCATGTTATATCTCGATGTTTTTATTATAAATCAAATAGATTTTCTTCAACCCGTTCAATTTCGGCTTGGAGTTTTTTGATAAAACGTAATTTATCCGTAATCGCCCGCGCCGTTTGCCATTGTTGTTCGTTAAGTGCGGTGGATAATTCCGTTAAAATGGCGCTGCGGGTTTGCTGAATTTCTTGGCTGAAAGCGGTTAATTCGTTGGCATTTTTTTGCTGtt                                                                                                                                                                   >  1:267188/1‑251 (MQ=255)                                                                                        ctctGCAATTTGAAGTAATGCCATGTTATATCTCGATGTTTTTATTATAAATCAAATAGATTTTCTTCAACCCGTTCAATTTCGGCTTGGAGTTTTTTGATAAAACGTAATTTATCCGTAATCGCCCGCGCCGTTTGCCATTGTTGTTCGTTAAGTGCGGTGGATAATTCCGTTAAAATGGCGCTGCGGGTTTGCTGAATTTCTTGGCTGAAAGCGGTTAATTCGTTGGCATTTTTTTGCTGTTCGatat                                                                                                                                                             <  1:9298/250‑1 (MQ=255)                                                                                          ctGCAATTTGAAGTAATGCCATGTTATATCTCGATGTTTTTATTATAAATCAAATAGATTTTCTTCAACCCGTTCAATTTCGGCTTGGAGTTTTTTGATAAAACGTAATTTATCCGTAATCGCCCGCGCCGTTTGCCATTGTTGTTCGTTAAGTGCGGTGGATAATTCCGTTAAAATGGCGCTGCGGGTTTGCTGAATTTCTTGGCTGAAAGCGGTTAATTCGTTGGCATTTTTTTGCTGTTCGATAtttt                                                                                                                                                          >  1:388681/1‑251 (MQ=255)                                                                                                   gAAGTAATGCCATGTTATATCTCGATGTTTTTATTATAAATCAAATAGATTTTCTTCAACCCGTTCAATTTCGGCTTGGAGTTTTTTGATAAAACGTAATTTATCCGTAATCGCCCGCGCCGTTTGCCATTGTTGTTCGTTAAGTGCGGTGGATAATTCCGTTAAAATGGCGCTGCGGGTTTGCTGAATTTCTTGGCTGAAAGCGGTTAATTCGTTGGCATTTTTTTGCTGTTCGATATTTTCCAGGGttt                                                                                                                                                 >  1:463898/1‑251 (MQ=255)                                                                                                         aTGCCATGTTATATCTCGATGTTTTTATTATAAATCAAATAGATTTTCTTCAACCCGTTCAATTTCGGCTTGGAGTTTTTTGATAAAACGTAATTTATCCGTAATCGCCCGCGCCGTTTGCCATTGTTGTTCGTTAAGTGCGGTGGATAATTCCGTTAAAATGGCGCTGCGGGTTTGCTGAATTTCTTGGc                                                                                                                                                                                                       >  1:56579/1‑191 (MQ=255)                                                                                                         aTGCCATGTTATATCTCGATGTTTTTATTATAAATCAAATAGATTTTCTTCAACCCGTTCAATTTCGGCTTGGAGTTTTTTGATAAAACGTAATTTATCCGTAATCGCCCGCGCCGTTTGCCATTGTTGTTCGTTAAGTGCGGTGGATAATTCCGTTAAAATGGCGCTGCGGGTTTGCTGAATTTCTTGGCTGAAAGCGGTTAATTCGTTGGCATTTTTTTGCTGTTCGATATTTTCCAGGGTTTCCCGcc                                                                                                                                           <  1:369416/251‑1 (MQ=255)                                                                                                              aTGTTATATCTCGATGTTTTTATTATAAATCAAATAGATTTTCTTCAACCCGTTCAATTTCGGCTTGGAGTTTTTTGATAAAACGTAATTTATCCGTAATCGCCCGCGCCGTTTGCCATTGTTGTTCGTTAAGTGCGGTGGATAATTCCGTTAAAATGGCGCTGCGGGTTTGCTGAATTTCTTGGCTGAAAGCGGTTAATTCGTTGGCATTTTTTTGCTGTTCGATATTTTCCAGGGTTTCCCGccattc                                                                                                                                       >  1:105964/1‑250 (MQ=255)                                                                                                              aTGTTATATCTCGATGTTTTTATTATAAATCAAATAGATTTTCTTCAACCCGTTCAATTTCGGCTTGGAGTTTTTTGATAAAACGTAATTTATCCGTAATCGCCCGCGCCGTTTGCCATTGTTGTTCGTTAAGTGCGGTGGATAATTCCGTTAAAATGGCGCTGCGGGTTTGCTGAATTTCTTGGCTGAAAGCGGTTAATTCGTTGGCATTTTTTTGCTGTTCGATATTTTCCAGGGTTTCCCGccattc                                                                                                                                       >  1:289090/1‑250 (MQ=255)                                                                                                                gTTATATCTCGATGTTTTTATTATAAATCAAATAGATTTTCTTCAACCCGTTCAATTTCGGCTTGGAGTTTTTTGATAAAACGTAATTTATCCGTAATCGCCCGCGCCGTTTGCCATTGTTGTTCGTTAAGTGCGGTGGATAATTCCGTTAAAATGGCGCTGCGTGTTTGCTGAATTTCTTGGCTGAAAGCGGTTAATTCGTTGGCAttttt                                                                                                                                                                           >  1:830837/1‑212 (MQ=255)                                                                                                                gTTATATCTCGATGTTTTTATTATAAATCAAATAGATTTTCTTCAACCCGTTCAATTTCGGCTTGGAGTTTTTTGATAAAACGTAATTTATCCGTAATCGCCCGCGCCGTTTGCCATTGTTGTTCGTTAAGTGCGGTGGATAATTCCGTTAAAATGGCGCTGCGGGTTTGCTGAATTTCTTGGCTGAAAGCGGTTAATTCGTTGGCATTTTTTTGCTGTTCGATATTTTCCAGGGt                                                                                                                                                   >  1:363655/1‑236 (MQ=255)                                                                                                                gTTATATCTCGATGTTTTTATTATAAATCAAATAGATTTTCTTCAACCCGTTCAATTTCGGCTTGGAGTTTTTTGATAAAACGTAATTTATCCGTAATCGCCCGCGCCGTTTGCCATTGTTGTTCGTTAAGTGCGGTGGATAATTCCGTTAAAATGGCGCTGCGGGTTTGCTGAATTTCTTGGCTGAAAGCGGTTAATTCGTTGGCATTTTTTTGCTGTTCGATATTTTCCAGGGTTTCCCGccattccat                                                                                                                                    >  1:885444/1‑251 (MQ=255)                                                                                                                     atCTCGATGTTTTTATTATAAATCAAATAGATTTTCTTCAACCCGTTCAATTTCGGCTTGGAGTTTTTTGATAAAACGTAATTTATCCGTAATCGCCCGCGCCGTTTGCCATTGTTGTTCGTTAAGTGCGGTGGATAATTCCGTTAAAATGGCGCTGCGGGTTTGCTGAATTTCTTGGCTGAAAGCGGTTAATTCGTTGGCATTTTTTTGCTGTTCGatat                                                                                                                                                             <  1:841982/221‑1 (MQ=255)                                                                                                                     atCTCGATGTTTTTATTATAAATCAAATAGATTTTCTTCAACCCGTTCAATTTCGGCTTGGAGTTTTTTGATAAAACGTAATTTATCCGTAATCGCCCGCGCCGTTTGCCATTGTTGTTCGTTAAGTGCGGTGGATAATTCCGTTAAAATGGCGCTGCGGGTTTGCTGAATTTCTTGGCTGAAAGCGGTTAATTCGTTGGCATTTTTTTGCTGTTCGATATTTTCCAGGGTTTCCCGCCATTCCATTtg                                                                                                                                 >  1:12878/1‑249 (MQ=255)                                                                                                                            tGTTTTTATTATAAATCAAATAGATTTTCTTCAACCCGTTCAATTTCGGCTTGGAGTTTTTTGATAAAACGTAATTTATCCGTAATCGCCCGCGCCGTTTGCCATTGTTGTTCGTTAAGTGCGGTGGATAATTCCGTTAAAATGGCGCTGCGGGTTTGCTGAATTTCTTGGCTGAAAGc                                                                                                                                                                                                >  1:93764/1‑179 (MQ=255)                                                                                                                               ttttATTATAAATCAAATAGATTTTCTTCAACCCGTTCAATTTCGGCTTGGAGTTTTTTGATAAAACGTAATTTATCCGTAATCGCCCGCGCCGTTTGCCATTGTTGTTCGTTAAGTGCGGTGGATAATTCCGTTAAAATGGCGCTGCGGGTTTGCTGAATTTCTTGGCTGAAAGCGGTTAATTCGTTGGCATTTTTTTGCTGTTCGATATTTTCCAGGGTTTCCCGCCATTCCATTTGCTGCATCaaaa                                                                                                                      <  1:292902/250‑1 (MQ=255)                                                                                                                                     tataAATCAAATAGATTTTCTTCAACCCGTTCAATTTCGGCTTGGAGTTTTTTGATAAAACGTAATTTATCCGTAATCGCCCGCGCCGTTTGCCATTGTTGTTCGTTAAGTGCGGTGGATAATTCCGTTAAAATGGCGCTGCGGGTTTGCTGAATTTCTTGGCTGAAAGCGGTTAAtt                                                                                                                                                                                        >  1:651124/1‑178 (MQ=255)                                                                                                                                        aaaTCAAATAGATTTTCTTCAACCCGTTCAATTTCGGCTTGGAGTTTTTTGATAAAACGTAATTTATCCGTAATCGCCCGCGCCGTTTGCCATTGTTGTTCGTTAAGTGCGGTGGATAATTCCGTTAAAATGGCGCTGCGGGTTTGCTGAATTTCTTGGCTGAAAGCGGTTAATTCGTTGGCATTTTTTTGCTGTTCGATATTTTCCAGGGTTTCCCGCCATTCCATTTGCTGCATCAAAAAGGCAATATc                                                                                                            <  1:620688/251‑1 (MQ=255)                                                                                                                                          aTCAAATAGATTTTCTTCAACCCGTTCAATTTCGGCTTGGAGTTTTTTGATAAAACGTAATTTATCCGTAATCGCCCGCGCCGTTTGCCATTGTTGTTCGTTAAGTGCGGTGGATAATTCCGTTAAAATGGCGCTGCGGGTTTGCTGAATTTCTTGGCTGAAAGCGGTTAATTCGTTGGCATTTTTTTGCTGTTCGATATTTTCCAggg                                                                                                                                                    >  1:252581/1‑209 (MQ=255)                                                                                                                                              aaTAGATTTTCTTCAACCCGTTCAATTTCGGCTTGGAGTTTTTTGATAAAACGTAATTTATCCGTAATCGCCCGCGCCGTTTGCCATTGTTGTTCGTTAAGTGCGGTGGATAATTCCGTTAAAATGGCGCTGCGGGTTTGCTGAATTTCTTGGCTGAAAGCGGCTAATTCGTTGGCATTTTTGTGCTGTTCGATATTTTCCGGGGTTTCCCGCCAGTCGAttt                                                                                                                                  >  1:818651/1‑223 (MQ=255)                                                                                                                                                  gATTTTCTTCAACCCGTTCAATTTCGGCTTGGGGTTTTTTGATAAAACGTAATTTATCCGTAATCGCCCGCGCCGTTTGCCATTGTTGTTCGTTAAGTGCGGTGGATAATTCCGTTAAAATGGCGCTGCGGGTTTGCTGAATTTCTTGGCTGAAAGCGGTTAATTCGTTGGCATTTTTTTGCTGTTCGATATTTTCCAGGGTTTCCCGCCATTCCATTTGCTGCATCAAAAAGGCAATATCATTGGTGCtt                                                                                                  >  1:867796/1‑251 (MQ=255)                                                                                                                                                  gATTTTCTTCAACCCGTTCAATTTCGGCTTGGAGTTTTTTGATAAAACGTAATTTATCCGTAATCGCCCGCGCCGTTTGCCATTGTTGTTCGTTAAGTGCGGTGGATAATTCCGTTAAAATGGCGCTGCGGGTTTGCTGAATTTCTTGGCTGAAAGCGGTTAATTCGTTGGCATTTTTTTGCTGTTCGATATTTTcc                                                                                                                                                        <  1:198766/197‑1 (MQ=255)                                                                                                                                                  gATTTTCTTCAACCCGTTCAATTTCGGCTTGGAGTTTTTTGATAAAACGTAATTTATCCGTAATCGCCCGCGCCGTTTGCCATTGTTGTTCGTTAAGTGCGGTGGATAATTCCGTTAAAATGGCGCTGCGGGTTTGCTGAATTTCTTGGCTGAAAGCGGTTAATTCGTTGGCATTTTTTTGCTGTTCGATATTTTCCAGGGTTTCCCGCCATTCCATTTGCTGCATCAAAAAGGCAATATCAt                                                                                                          <  1:144019/243‑1 (MQ=255)                                                                                                                                                  gATTTTCTTCAACCCGTTCAATTTCGGCTTGGAGTTTTTTGATAAAACGTAATTTATCCGTAATCGCCCGCGCCGTTTGCCATTGTTGTTCGTTAAGTGCGGTGGATAATTCCGTTAAAATGGCGCTGCGGGTTTGCTGAATTTCTTGGCTGAAAGCGGTTAATTCGTTGGCATTTTTTTGCTGTTCGATATTTTCCAGGGTTTCCCGCCATTCCATTTGCTGCATCAAAAAGGCAATATCATTGGTGCtt                                                                                                  >  1:533172/1‑251 (MQ=255)                                                                                                                                                  gATTTTCTTCAACCCGTTCAATTTCGGCTTGGAGTTTTTTGATAAAACGTAATTTATCCGTAATCGCCCGCGCCGTTTGCCATTGTTGTTCGTTAAGTGCGGTGGATAATTCCGTTAAAATGGCGCTGCGGGTTTGCTGAATTTCTTGGCTGAAAGCGGTTAATTCGTTGGCATTTTTTTGCTGTTCGATATTTTCCAGGGTTTCCCGCCATTCCATTTGCTGCATCAAAAAGGCAATATCATTGGTGCtt                                                                                                  >  1:30330/1‑251 (MQ=255)                                                                                                                                                   aTTTTCTTCAACCCGTTCAATTTCGGCTTGGAGTTTTTTGATAAAACGTAATTTATCCGTAATCGCCCGCGCCGTTTGCCATTGTTGTTCGTTAAGTGCGGTGGATAATTCCGTTAAAATGGCGCTGCGGGTTTGCTGAATTTCTTGGCTGAAAGCGGTTAATTCGTTGGCATTTTTTTGCTGTTCGATATTTTCCAGGGTTTCCCGCCATTCCATTTGCTGCATCAAAAAGGCAATATCATTGGTGCttt                                                                                                 >  1:715375/1‑251 (MQ=255)                                                                                                                                                       tcttcAACCCGTTCAATTTCGGCTTGGAGTTTTTTGATAAAACGTAATTTATCCGTAATCGCCCGCGCCGTTTGCCATTGTTGTTCGTTAAGTGCGGTGGATAATTCCGTTAAAATGGCGCTGCGGGTTTGCTGAATTTCTTGGCTGAAAGCGGTTAATTCGTTGGCAtttttt                                                                                                                                                                          >  1:824682/1‑174 (MQ=255)                                                                                                                                                       tcttcAACCCGTTCAATTTCGGCTTGGAGTTTTTTGATAAAACGTAATTTATCCGTAATCGCCCGCGCCGTTTGCCATTGTTGTTCGTTAAGTGCGGTGGATAATTCCGTTAAAATGGCGCTGCGGGTTTGCTGAATTTCTTGGCTGAAAGCGGTTAATTCGTTGGCATTTTTTTGCTGTTCGATATTTTCCAGGGTTTCCCGCCATTCCAttt                                                                                                                                  >  1:309558/1‑214 (MQ=255)                                                                                                                                                       tcttcAACCCGTTCAATTTCGGCTTGGAGTTTTTTGATAAAACGTAATTTATCCGTAATCGCCCGCGCCGTTTGCCATTGTTGTTCGTTAAGTGCGGTGGATAATTCCGTTAAAATGGCGCTGCGGGTTTGCTGAATTTCTTGGCTGAAAGCGGTTAATTCGTTGGCATTTTTTTGCTGTTCGATATTTTCCAGGGTTTCCCGCCATTCCATTTGCTGCATCAAAAAGGCAATATCATTGGTGCtttttt                                                                                              >  1:554117/1‑250 (MQ=255)                                                                                                                                                       tcttcAACCCGTTCAATTTCGGCTTGGAGTTTTTTGATAAAACGTAATTTATCCGTAATCGCCCGCGCCGTTTGCCATTGTTGTTCGTTAAGTGCGGTGGATAATTCCGTTAAAATGGCGCTGCGGGTTTGCTGAATTTCTTGGCTGAAAGCGGTTAATTCGTTGGCATTTTTTTGCTGTTCGATATTTTCCAGGGTTTCCCGCCATTCCATTTGCTGCATCAAAAAGGCAATATCATTGGTGCtttttt                                                                                              >  1:29812/1‑250 (MQ=255)                                                                                                                                                       tcttcAACCCGTTCAATTTCGGCTTGGAGTTTTTTGATAAAACGTAATTTATCCGTAATCGCCCGCGCCGTTTGCCATTGTTGTTCGTTAAGTGCGGTGGATAATTCCGTTAAAATGGCGCTGCGGGTTTGCTGAATTTCTTGGCTGAAAGCGGTTAATTCGTTGGCATTTTTTTGCTGTTCGATATTTTCCAGGGTTTCCCGCCATTCCATTTGCTGCATCAAAAAGGCAATATCATTGGTGCtttttt                                                                                              >  1:652915/1‑250 (MQ=255)                                                                                                                                                       tcttcAACCCGTTCAATTTCGGCTTGGAGTTTTTTGATAAAACGTAATTTATCCGTAATCGCCCGCGCCGTTTGCCATTGTTGTTCGTTAAGTGCGGTGGATAATTCCGTTAAAATGGCGCTGCGGGTTTGCTGAATTTCTTGGCTGAAAGCGGTTAATTCGTTGGCATTTTTTTGCTGTTCGATATTTTCCAGGGTTTCCCGCCATTCCATTTGCTGCATCAAAAAGGCAATATCATTGGTGCtttttt                                                                                              >  1:355446/1‑250 (MQ=255)                                                                                                                                                       tcttcAACCCGTTCAATTTCGGCTTGGAGTTTTTTGATAAAACGTAATTTATCCGTAATCGCCCGCGCCGTTTGCCATTGTTGTTCGTTAAGTGCGGTGGATAATTCCGTTAAAATGGCGCTGCGGGTTTGCTGAATTTCTTGGCTGAAAGCGGTTAATTCGTTGGCATTTTTTTGCTGTTCGATATTTTCCAGGGTTTCCCGCCATTCCATTTGCTGCATCAAAAAGGCAATATCATTGGTGCtttttt                                                                                              >  1:712801/1‑250 (MQ=255)                                                                                                                                                        cttcAACCCGTTCAATTTCGGCTTGGAGTTTTTTGATAAAACGTAATTTATCCGTAATCGCCCGCGCCGTTTGCCATTGTTGTTCGTTAAGTGCGGTGGATAATTCCGTTAAAATGGCGCTGCGGGTTTGCTGAATTTCTTGGCTGAAAGCGGTTAATTCGTTGGCATTTTTTTGCTGTTCGATATTTTCCAGGGTTTCCCGCCATTCCATTTGCTGCATCAAAAAGGCAATATc                                                                                                            <  1:508373/235‑1 (MQ=255)                                                                                                                                                                 gTTCAATTTCGGCTTGGAGTTTTTTGATAAAACGTAATTTATCCGTAATCGCCCGCGCCGTTTGCCATTGTTGTTCGTTAAGTGCGGTGGATAATTCCGTTAAAATGGCGCTGCGGGTTTGCTGAATTTCTTGGCTGAAAGCGGTTAATTCGTTGGCATTTTTTTGCTGTTCGATATTTTCCAGGGTTTCCCGCCATTCCATTTGCTGCATCAAAAAGGCAATATCATTgg                                                                                                       >  1:627868/1‑231 (MQ=255)                                                                                                                                                                                      ttttGATAAAACGTAATTTATCCGTAATCGCCCGCGCCGTTTGCCATTGTTGTTCGTTAAGTGCGGTGGATAATTCCGTTAAAATGGCGCTGCGGGTTTGCTGAATTTCTTGGCTGAAAGCGGTTAATTCGTTGGCATTTTTTTGCTGTTCGATATTTTCCAGGGTTTCCCGCCATTCCATTTGCTGCATCAAAAAGGCAATATCATTGGTGCTTTTTTCTTCTACGTTTTCCGTTTCGCCGGTGTTGATg                                                              <  1:550285/251‑1 (MQ=255)                                                                                                                                                                                           aTAAAACGTAATTTATCCGTAATCGCCCGCGCCGTTTGCCATTGTTGTTCGCTAAGTGCGGTGGATAATTCCGTTAAAATGGCGCTGCGGGTTTGCTGAATTTCTTGGCTGAAAGCGGTTAATTCGTTGGCATTTTTTTGCTGTTCGATATTTTCCAGGGTTTCCCGCCATTCCATTTGCTGCATCAAAAAGGCAATATCATTGGTGCTTTTTTCTTCTACGTTTTCCGTTTCGCCGGTGTTGATGTCGAt                                                         <  1:626572/251‑1 (MQ=255)                                                                                                                                                                                               aaCGTAATTTATCCGTAATCGCCCGCGCCGTTTGCCATTGTTGTTCGTTAAGTGCGGTGGATAATTCCGTTAAAATGGCGCTGCGGGTTTGCTGAATTTCTTGGCTGAAAGCGGTTAATTCGTTGGCATTTTTTTGCTGTTCGATATTTTCCAg                                                                                                                                                      <  1:95302/154‑1 (MQ=255)                                                                                                                                                                                               aaCGTAATTTATCCGTAATCGCCCGCGCCGTTTGCCATTGTTGTTCGTTAAGTGCGGTGGATAATTCCGTTAAAATGGCGCTGCGGGTTTGCTGAATTTCTTGGCTGAAAGCGGTTAATTCGTTGGCATTTTTTTGCTGTTCGATATTTTCCAGGGTTTCCCGCCATTCCATTTGCTGCATCAAAAAGGCAATATCATTGGTGCTTTTttcttc                                                                                          >  1:11816/1‑214 (MQ=255)                                                                                                                                                                                               aaCGTAATTTATCCGTAATCGCCCGCGCCGTTTGCCATTGTTGTTCGTTAAGTGCGGTGGATAATTCCGTTAAAATGGCGCTGCGGGTTTGCTGAATTTCTTGGCTGAAAGCGGTTAATTCGTTGGCATTTTTTTGCTGTTCGATATTTTCCAGGGTTTCCCGCCATTCCATTTGCTGCATCAAAAAGGCAATATCATTGGTGCTTTTTTCTTCTACGTTTTCCGTTTCGCCGGTGTTGATg                                                              <  1:235209/242‑1 (MQ=255)                                                                                                                                                                                                aCGTAATTTATCCGTAATCGCCCGCGCCGTTTGCCATTGTTGTTCGTTAAGTGCGGTGGATAATTCCGTTAAAATGGCGCTGCGGGTTTGCTGAATTTCTTGGCTGAAAGCGGTTAATTCGTTGGCATTTTTTTGCTGTTCGATATTTTCCAGGGTTTCCCGCCATTCCATTTGCTGCATCAAAAAGGCAATATCATTGGTGCTTTTTTCTTCTACGTTTTCCGTTTCGCCGGTGTTGATGTCGATAATGc                                                    <  1:739659/251‑1 (MQ=255)                                                                                                                                                                                                aCGTAATTTATCCGTAATCGCCCGCGCCGTTTGCCATTGTTGTTCGTTAAGTGCGGTGGATAATTCCGTTAAAATGGCGCTGCGGGTTTGCTGAATTTCTTGGCTGAAAGCGGTTAATTCGTTGGCATTTTTTTGCTGTTCGATATTTTCCAGGGTTTCCCGCCATTCCATTTGCTGCATCAAAAAGGCAATATCATTGGTGCTTTTTTCTTCTACGTTTTCCGTTTCGCCGGTGTTGATGTCGATAATGc                                                    <  1:264165/251‑1 (MQ=255)                                                                                                                                                                                                  gTAATTTATCCGTAATCGCCCGCGCCGTTTGCCATTGTTGTTCGTTAAGTGCGGTGGATAATTCCGTTAAAATGGCGCTGCGGGTTTGCTGAATTTCTTGGCTGAAAGCGGTTAATTCGTTGGCATTTTTTTGCTGTTCGATATTTTCCAGGGTTTCCCGCCATTCCATTTGCTGCATCAAAAAGGCAATATCATTGGTGCTTTTTTCTTCTACGTTTTCCGTTTCGCCGGTGTTGATGTCGATAATGc                                                    >  1:736672/1‑249 (MQ=255)                                                                                                                                                                                                     aTTTATCCGTAATCGCCCGCGCCGTTTGCCATTGTTGTTCGTTAAGTGCGGTGGATAATTCCGTTAAAATGGCGCTGCGGGTTTGCTGAATTTCTTGGCTGAAAGCGGTTAATTCGTTGGCATTTTTTTGCTGTTCGATATTTTCCAGGGTTTCCCGCCATTCCATTTGCTGCATCAAAAAGGCAATATCATTGGTGCTTTTTTCTTCTACGTTTTCCGTTTCGCCGGTGTTGATGTCGATAATGCTGTCg                                               >  1:247329/1‑251 (MQ=255)                                                                                                                                                                                                        tATCCGTAATCGCCCGCGCCGTTTGCCATTGTTGTTCGTTAAGTGCGGTGGATAATTCCGTTAAAATGGCGCTGCGGGTTTGCTGAATTTCTTGGCTGAAAGCGGTTAATTCGTTGGCATTTTTTTGCTGTTCGATATTTTCCGGGGTTTCCCGCCATTCCATTTGCTGCATCAAAAAGGCAATATCATTGGTGCTTTTTTCTTCTACGTTTTCCGTTTCGCCGGTGTTGATGTCGATAATGCTGTCGgcg                                            <  1:715529/251‑1 (MQ=255)                                                                                                                                                                                                         aTCCGTAATCGCCCGCGCCGTTTGCCATTGTTGTTCGTTAAGTGCGGTGGATAATTCCGTTAAAATGGCGCTGCGGGTTTGCTGAATTTCTTGGCTGAAAGCGGTTAATTCGTTGGCATTTTTTTGCTGTTCGATATTTTCCAGGGTTTCCCGCCATTCCATTTGCTGCATCAAAAAGGCAATATCATTGGTGCTTTTTTCTTCTACGTTTTCCGTTTCGCCGGTGTTGATGTCGATAATGCTGTCGgcg                                            >  1:395106/1‑250 (MQ=255)                                                                                                                                                                                                                  cGCCCGCGCCGTTTGCCATTGTTGTTCGTTAAGTGCGGTGGATAATTCCGTTAAAATGGCGCTGCGGGTTTGCTGAATTTCTTGGCTGAAAGCGGTTAATTCGTTGGCATTTTTTTGCTGTTCGATATTTTCCAGGGTTTCCCGCCATTCCATTTGCTGCATCAAAAAGGCAATATCATTGGTGCTTTTTTCTTCTACGTTTTCCGTTTCGCCGGTGTTGATGTCGATAATGCTGTCGGCGCGTGCGATgg                                  >  1:391433/1‑251 (MQ=255)                                                                                                                                                                                                                     ccGCGCCGTTTGCCATTGTTGTTCGTTAAGTGCGGTGGATAATTCCGTTAAAATGGCGCTGCGGGTTTGCTGAATTTCTTGGCTGAAAGCGGTTAATTCGTTGGCATTTTTTTGCTGTTCGATATTTTCCAGGGTTTCCCGCCATTCCATTTGCTGCATCAAAAAGGCAATATCATTGGTGCTTTTTTCTTCTACGTTTTCCGTTTCGCCGGTGTTGATGTCGATAATGCTGTCGGCGCGTGCGATGGGAt                               <  1:596231/251‑1 (MQ=255)                                                                                                                                                                                                                          ccGTTTGCCATTGTTGTTCGTTAAGTGCGGTGGATAATTCCGTTAAAATGGCGCTGCGGGTTTGCTGAATTTCTTGGCTGAAAGCGGTTAATTCGTTGGCATTTTTTTGCTGTTCGATATTTTCCAGGGTTTcc                                                                                                                                               >  1:25950/1‑134 (MQ=255)                                                                                                                                                                                                                            gTTTGCCATTGTTGTTCGTTAAGTGCGGTGGATAATTCCGTTAAAATGGCGCTGCGGGTTTGCTGAATTTCTTGGCTGAAAGCGGTTAATTCGTTGGCATTTTTTTGCTGTTCGATATTTTCCAGGGTTTCCCGCCATTCCATTTGCTGCATCAAAAAGGCAATATCATTGGTGCTTTTTTCTTCTACGTTTTCCGTTTCGCCGGTGTTGATGTCGATAATGCTGTCGGCGCGTGCGATGGGATCTTTTaa                        >  1:688003/1‑251 (MQ=255)                                                                                                                                                                                                                              ttGCCATTGTTGTTCGTTAAGTGCGGTGGATAATTCCGTTAAAATGGCGCTGCGGGTTTGCTGAATTTCTTGGCTGAAAGCGGTTAATTCGTTGGCATTTTTTTGCTGTTCGATATTTTCCGGGGTTTCCCGCCATTCCATTTGCTGCATCAAAAAGGCAATATCATTGGTGCTTTTTTCTTCTACGTTTTCCGTTTCGCCGGTGTTGATGTCGATAATGCTGTCGGCGCGTGCGATGGGATCTTTTaaaa                      <  1:879601/251‑1 (MQ=255)                                                                                                                                                                                                                              ttGCCATTGTTGTTCGTTAAGTGCGGTGGATAATTCCGTTAAAATGGCGCTGCGGGTTTGCTGAATTTCTTGGCTGAAAGCGGTTAATTCGTTGGCATTTTTTTGCTGTTCGATATTTTCCAGGGTTTCCCGCCATTCCATTTGCTGCATCAAAAAGGCAATATCATTGGTGCTTTTTTCTTCTACGTTTTCCGTTTCGCCGGTGTTGATGTCGATAATGCTGTCGGCGCGTGCGATGGGATCTTTTaaaa                      <  1:88598/251‑1 (MQ=255)                                                                                                                                                                                                                                      gttgttCGTTAAGTGCGGTGGATAATTCCGTTAAAATGGCGCTGCGGGTTTGCTGAATTTCTTGGCTGAAAGCGGTTAATTCGTTGGCATTTTTTTGCTGTTCGATATTTTCCAGGGTTTCCCGCCATTCCATTtgc                                                                                                                                >  1:355195/1‑137 (MQ=255)                                                                                                                                                                                                                                             gTTAAGTGCGGTGGATAATTCCGTTAAAATGGCGCTGCGGGTTTGCTGAATTTCTTGGCTGAAAGCGGTTAATTCGTTGGCATTTTTTTGCTGTTCGATATTTTCCAGGGTTTCCCGCCATTCCATTTGCTGCATCAAAAAGGCAATATCATTGGTGCTTTTTTCTTCTACGTTTTCCGTTTCGCCGGTGTTGATGTCGATAATGCTGTCGGCGCGTGCGATGGGATCTTTTAAAATGCGCAGGGcatcat       >  1:601758/1‑251 (MQ=255)                                                                                                                                                                                                                                              ttAAGTGCGGTGGATAATTCCGTTAAAATGGCGCTGCGGGTTTGCTGAATTTCTTGGCTGAAAGCGGTTAATTCGTTGGCATTTTTTTGCTGTTCGATATTTTCCAGGGTTTCCCGCCATTCCATTTGCTGCATCAAAAAGGCAATATCATTGGTGCTTTTTTCTTCTACGTTTTCCGTTTCGCCGGTGTTGATGTCGATAATGCTGTCGGCGCGTGCGATGGGATCTTTTAAAATGCGCAGGGcatcat       <  1:425437/250‑1 (MQ=255)                                                                                                                                                                                                                                                  gTGCGGTGGATAATTCCGTTAAAATGGCGCTGCGGGTTTGCTGAATTTCTTGGCTGAAAGCGGTTAATTCGTTGGCATTTTTTTGCTGTTCGATATTTTCCAGGGTTTCCCGCCATTCCATTTGCTGCATCAAAAAGGCAATATCATTGGTGCTTTTTTCTTCTACGTTTTCCGTTTCGCCGGTGTTGATGTCGATAATGCTGTCGGCGCGTGCGATGGGATCTTTTAAAATGCGCAGGGCATCATTAAtt  >  1:572507/1‑251 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                     GCGCACGGAGGCAATGAGGGAATTGGTGGTGCCGAGATCAATGCCTACCGCCAGTTTATGTTGATGCGGTGCGGCGCTTTGACCGGGCTCTGCAATTTGAAGTAATGCCATGTTATATCTCGATGTTTTTATTATAAATCAAATAGATTTTCTTCAACCCGTTCAATTTCGGCTTGGAGTTTTTTGATAAAACGTAATTTATCCGTAATCGCCCGCGCCGTTTGCCATTGTTGTTCGTTAAGTGCGGCGGATAATTCCGTTAAAATGGCGCTGCGGGTTTGCTGAATTTCTTGGCTGAAAGCGGTTAATTCGTTGGCATTTTTTTGCTGTTCGATATTTTCCAGGGTTTCCCGCCATTCCATTTGCTGCATCAAAAAGGCAATATCATTGGTGCTTTTTTCTTCTACGTTTTCCGTTTCGCCGGTGTTGATGTCGATAATGCTGTCGGCGCGTGCGATGGGATCTTTTAAAATGCGCAGGGCATCATTAATT  >  1/971335‑971826

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑