Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	851,190	A→G	noncoding (51/77 nt)	unknown ←	tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	851,190	0	A	G	100.0%	143.3 / NA	41	noncoding (51/77 nt)	unknown	tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (16/25);  total (16/25)

AAACCAACTCTTTTGTCTACTATGCCTATTTTTAGTACCTAGAAAATCACTAAATTCCCCTGATAGGGTACTATATCAGGTACTAAAGGTAAAACTAAAATGAATCAAAAAAAGCATAATACACTATGGAAATGATTAAATATGTTAATTTATAAGTGGTTTTGGTTTGACTTGAATCAGAGTGAAGTAGTATGGATTAATATGTTGGTGCCCCCAACAGGACTTGAACCTATGACTAATCGATTATGAGTCGACTGCTCTGACCGACTGAGCTATGGGGGCGGTGTGAGCGTAGAATTAAGGCGCCATTATAGGGAAATATTGGGGTAAAGTCCAGAATATTATGTGTTATTTAAAGTGCGGTTGCTTTTACCGGCGTTTTTAATGTTCAAAAACGTGGAAAAATTATTTGCCCTTTGAAAAGTCAGGTTGTTCCTTGTGATTTTAGCTGATTAGCTTTTG  >  1/850959‑851420                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                       aaaCCAACTCTTTTGTCTACTATGCCTATTTTTAGTACCTAGAAAATCACTAAATTCCCCTGATAGGGTACTATATCAGGTACTAAAGGTAAAACTAAAATGAATCAAAAAAAGCATAATACACTATGGAAATGATTAAATATGTTAATTTATAAGTGGTTTTGGTTTGACTTGAATCAGAGTGAAGTAGTATGGATTAATATGTTGGTGCCCCCAACAGGACTTGAACCTGTGACTAATCGATTATGAGt                                                                                                                                                                                                                     <  1:293933/251‑1 (MQ=255)                               tttAGTACCTAGAAAATCACTAAATTCCCCTGATAGGGTACTATATCAGGTACTAAAGGTAAAACTAAAATGAATCAAAAAAAGCATAATACACTATGGAAATGATTAAATATGTTAATTTATAAGTGGTTTTGGTTTGACTTGAATCAGAGTGAAGTAGTATGGATTAATATGTTGGTGCCCCCAACAGGACTTGAACCTGTGACTAATCGATTATGAGTCGACTGCTCTGACCGACTGAGCTATggggg                                                                                                                                                                                       <  1:363407/251‑1 (MQ=255)                                                  cTAAATTCCCCTGATAGGGTACTATATCAGGTACTAAAGGTAAAACTAAAATGAATCAAAAAAAGCATAATACACTATGGAAATGATTAAATATGTTAATTTATAAGTGGTTTTGGTTTGACTTGAATCAGAGTGAAGTAGTATGGATTAATATGTTGGTGCCCCCAACAGGACTTGAACCTGTGACTAATCGATTATGAGTCGACTGCTCTGACCGACTGAGCTATgggctgtctcttatacacattccg                                                                                                                                                                    >  1:604938/1‑230 (MQ=255)                                                                  gggTACTATATCAGGTACTAAAGGTAAAACTAAAATGAATCAAAAAAAGCATAATACACTATGGAAATGATTAAATATGTTAATTTATAAGTGGTTTTGGTTTGACTTGAATCAGAGTGAAGTAGTATGGATTAATATGTTGGTGCCCCCAACAGGACTTGAACCTGTGACTAATCGATTATGAGTCGACTGCTCTGACCGACTGAGCTATGGGGGCGGTGTGAGc                                                                                                                                                                             <  1:199024/226‑1 (MQ=255)                                                                  gggTACTATATCAGGTACTAAAGGTAAAACTAAAATGAATCAAAAAAAGCATAATACACTATGGAAATGATTAAATATGTTAATTTATAAGTGGTTTTGGTTTGACTTGAATCAGAGTGAAGTAGTATGGATTAATATGTTGGTGCCCCCAACAGGACTTGAACCTGTGACTAATCGATTATGAGTCGACTGCTCTGACCGACTGAGCTATGGGGGCGGTGTGAGCGTAGAATTAAGGCGCCATTATAggg                                                                                                                                                    >  1:699306/1‑251 (MQ=255)                                                                    gTACTATATCAGGTACTAAAGGTAAAACTAAAATGAATCAAAAAAAGCATAATACACTATGGAAATGATTAAATATGTTAATTTATAAGTGGTTTTGGTTTGACTTGAATCAGAGTGAAGTAGTATGGATTAATATGTTGGTGCCCCCAACAGGACTTGAACCTGTGACTAATCGATTATGAGTCGACTGCTCTGACCGACTGAGCTATGGGGGCGGTGTGAGCGTAGAATTAAGGCGCCATTATAGGGaa                                                                                                                                                  >  1:486240/1‑251 (MQ=255)                                                                           atCAGGTACTAAAGGTAAAACTAAAATGAATCAAAAAAAGCATAATACACTATGGAAATGATTAAATATGTTAATTTATAAGTGGTTTTGGTTTGACTTGAATCAGAGTGAAGTAGTATGGATTAATATGTTGGTGCCCCCAACAGGACTTGAACCTGTGACTAATCGATTATGAGTCGACTGCTCTGACCGACTGAGCTATGGGGGCGGTGTGAGCGTAGAATTAAGGCGCCATTATAGGGAAATATTgg                                                                                                                                           >  1:701487/1‑251 (MQ=255)                                                                           atCAGGTACTAAAGGTAAAACTAAAATGAATCAAAAAAAGCATAATACACTATGGAAATGATTAAATATGTTAATTTATAAGTGGTTTTGGTTTGACTTGAATCAGAGTGAAGTAGTATGGATTAATATGTTGGTGCCCCCAACAGGACTTGAACCTGTGACTAATCGATTATGAGTCGACTGCTCTGACCGACTGAGCTATGGGGGCGGTGTGAGCGTAGAATTAAGGCGCCATTATAGGGAAATATTgg                                                                                                                                           >  1:386206/1‑251 (MQ=255)                                                                                             aaCTAAAATGAATCAAAAAAAGCATAATACACTATGGAAATGATTAAATATGTTAATTTATAAGTGGTTTTGGTTTGACTTGAATCAGAGTGAAGTAGTATGGATTAATATGTTGGTGCCCCCAACAGGACTTGAACCTGTGACTAATCGATTATGAGTCGACTGCTCTGACCGACTGAGCTATGGGGGCGGTGTGAGCGTAGAATTAAGGCGCCATTATAGGGAAATATTGGGGTAAAGTCCAGAat                                                                                                                            <  1:289762/248‑1 (MQ=255)                                                                                                   aaTGAATCAAAAAAAGCATAATACACTATGGAAATGATTAAATATGTTAATTTATAAGTGGTTTTGGTTTGACTTGAATCAGAGTGAAGTAGTATGGATTAATATGTTGGTGCCCCCAACAGGACTTGAACCTGTGACTAATCGATTATGAGTCGACTGCTCTGACCGACTGAGCTATGGGGGCGGTGTGAGCGTAGAATTAAGGCGCCATTATAGGGAAATATTGGGGTAAAGTCCAGAATATTATgtgt                                                                                                                   <  1:755643/251‑1 (MQ=255)                                                                                                       aaTCAAAAAAAGCATAATACACTATGGAAATGATTAAATATGTTAATTTATAAGTGGTTTTGGTTTGACTTGAATCAGAGTGAAGTAGTATGGATTAATATGTTGGTGCCCCCAACAGGACTTGAACCTGTGACTAATCGATTATGAGTCGACTGCTCTGACCGACTGAGCTATGGGGGCGGTGTGAGCGTAGAATTAAGGCGCCATTATAGGGAAATATTGGGGTAAAGTCCAGAATATTATGTGTTAt                                                                                                                <  1:294150/250‑1 (MQ=255)                                                                                                                  gCATAATACACTATGGAAATGATTAAATATGTTAATTTATAAGTGGTTTTGGTTTGACTTGAATCAGAGTGAAGTAGTATGGATTAATATGTTGGTGCCCCCAACAGGACTTGAACCTGTGACTAATCGATTATGAGTCGACTGCTCTGACCGACTGAGCTATGGGGGCGGTGTGAGCGTAGAAt                                                                                                                                                                      <  1:555756/185‑1 (MQ=255)                                                                                                                   cATAATACACTATGGAAATGATTAAATATGTTAATTTATAAGTGGTTTTGGTTTGACTTGAATCAGAGTGAAGTAGTATGGATTAATATGTTGGTGCCCCCAACAGGACTTGAACCTGTGACTAATCGATTATGAGTCGACTGCTCTGACCGACTGAGCTATGGGGGCGGTGTGAGCGTAGAATTAAGGCGCCATTATAGGGAAATATTGGGGTAAAGTCCAGAATATTATGTGTTATTTAAAg                                                                                                          >  1:833255/1‑244 (MQ=255)                                                                                                                         acacTATGGAAATGATTAAATATGTTAATTTATAAGTGGTTTTGGTTTGACTTGAATCAGAGTGAAGTAGTATGGATTAATATGTTGGTGCCCCCAACAGGACTTGAACCTGTGACTAATCGATTATGAGTCGACTGCTCTGACCGACTGAGCTATGGGGGCGGTGTGAGCGTAGAATTAAGGCGCCATTATAGGGAAATATTGGGGTAAAGTCCAGa                                                                                                                              <  1:128652/218‑1 (MQ=255)                                                                                                                            cTATGGAAATGATTAAATATGTTAATTTATAAGTGGTTTTGGTTTGACTTGAATCAGAGTGAAGTAGTATGGATTAATATGTTGGTGCCCCCAACAGGACTTGAACCTGTGACTAATCGATTATGAGTCGACTGCTCTGACCGACTGAGCTATGGGGGCGGtgtg                                                                                                                                                                                <  1:105150/165‑1 (MQ=255)                                                                                                                            cTATGGAAATGATTAAATATGTTAATTTATAAGTGGTTTTGGTTTGACTTGAATCAGAGTGAAGTAGTATGGATTAATATGTTGGTGCCCCCAACAGGACTTGAACCTGTGACTAATCGATTATGAGTCGACTGCTCTGACCGACTGAGCTATGGGGGCGGTGTGAGCGTAGAATTAAGGCGCCATTATAGGGAAATATTGGGGTAAAGTCCAGAATATTAtg                                                                                                                      >  1:469927/1‑223 (MQ=255)                                                                                                                            cTATGGAAATGATTAAATATGTTAATTTATAAGTGGTTTTGGTTTGACTTGAATCAGAGTGAAGTAGTATGGATTAATATGTTGGTGCCCCCAACAGGACTTGAACCTGTGACTAATCGATTATGAGTCGACTGCTCTGACCGACTGAGCTATGGGGGCGGTGTGAGCGTAGAATTAAGGCGCCATTATAGGGAAATATTGGGGTAAAGTCCAGAATATTATGTGTTATTTAAAGTGCGGTTGCTTTTAcc                                                                                          <  1:650596/251‑1 (MQ=255)                                                                                                                                ggAAATGATTAAATATGTTAATTTATAAGTGGTTTTGGTTTGACTTGAATCAGAGTGAAGTAGTATGGATTAATATGTTGGTGCCCCCAACAGGACTTGAACCTGTGACTAATCGATTATGAGTCGACTGCTCTGACCGACTGAGCTATGGGGGCGGTGTGAGCGTAGAATTAAGGCGCCATTATAGGGAAATATTgggg                                                                                                                                         >  1:335711/1‑200 (MQ=255)                                                                                                                                      gATTAAATATGTTAATTTATAAGTGGTTTTGGTTTGACTTGAATCAGAGTGAAGTAGTATGGATTAATATGTTGGTGCCCCCAACAGGACTTGAACCTGTGACTAATCGATTATGAGTCGACTGCTCTGACCGACTGAGCTATGGGGGCGGTGTGAGCGTAGAATTAAGGCGCCATTATAGGGAAatat                                                                                                                                              <  1:152740/189‑1 (MQ=255)                                                                                                                                                    aTTTATAAGTGGTTTTGGTTTGACTTGAATCAGAGTGAAGTAGTATGGATTAATATGTTGGTGCCCCCAACAGGACTTGAACCTGTGACTAATCGATTATGAGTCGACTGCTCTGACCGACTGAGCTATGGGGGCGGTGTGAGCGTAGAATTAAGGCGCCATTATAGGGAAATATTGGGGTAAAGTCCAGAat                                                                                                                            >  1:79931/1‑193 (MQ=255)                                                                                                                                                     tttATAAGTGGTTTTGGTTTGACTTGAATCAGAGTGAAGTAGTATGGATTAATATGTTGGTGCCCCCAACAGGACTTGAACCTGTGACTAATCGATTATGAGTCGACTGCTCTGACCGACTGAGCTATGGGGGCGGTGTGAGCGt                                                                                                                                                                           <  1:748411/145‑1 (MQ=255)                                                                                                                                                                    ggtttGACTTGAATCAGAGTGAAGTAGTATGGATTAATATGTTGGTGCCCCCAACAGGACTTGAACCTGTGACTAATCGATTATGAGTCGACTGCTCTGACCGACTGAGCTATGGGGGCGGTGTGAGCGTAGAATTAAGGCGCCATTATAGGGAAATATTGGGGTAAAGTCCAGAatat                                                                                                                          <  1:362098/179‑1 (MQ=255)                                                                                                                                                                                   agagTGAAGTAGTATGGATTAATATGTTGGTGCCCCCAACAGGACTTGAACCTGTGACTAATCGATTATGAGTCGACTGCTCTGACCGACTGAGCTATGGGGGCGGTGTGAGCGTAGAATTAAGGCGCCATTATAGGGAAATATTGGGGTAAAGTCCAGAATATTAtg                                                                                                                      <  1:378896/168‑1 (MQ=255)                                                                                                                                                                                    gagTGAAGTAGTATGGATTAATATGTTGGTGCCCCCAACAGGACTTGAACCTGTGACTAATCGATTATGAGTCGACTGCTCTGACCGACTGAGCTATggggg                                                                                                                                                                                       <  1:284114/102‑1 (MQ=255)                                                                                                                                                                                    gagTGAAGTAGTATGGATTAATATGTTGGTGCCCCCAACAGGACTTGAACCTGTGACTAATCGATTATGAGTCGACTGCTCTGACCGACTGAGCTATGGGGGCGGTGTGAGCGTAGAATTAAGGCGCCATTATAgg                                                                                                                                                     >  1:667713/1‑136 (MQ=255)                                                                                                                                                                                     agTGAAGTAGTATGGATTAATATGTTGGTGCCCCCAACAGGACTTGAACCTGTGACTAATCGATTATGAGTCGACTGCTCTGACCGACTGAGCTATGGGGGCGGTGTGAGCGTAGAATTAAGGCGCCATTATAGGGAAATATTGGGGTAAAGTCCAGAATATTATGTGTTATTTAAAGTGCGGTTGCTTTTACCGGCGTTTTTAATGTTCAAAAACGTGGAAAAATTATTTGCCCTTTGAAAAGTCAGgt                                  <  1:338876/250‑1 (MQ=255)                                                                                                                                                                                         aaGTAGTATGGATTAATATGTTGGTGCCCCCAACAGGACTTGAACCTGTGACTAATCGATTATGAGTCGACTGCTCTGACCGACTGAGCTATGGGGGCGGTGTGAGCGTAGAATTAAGGCGCCATTATAGGGAAATATTGGGGTAAAGTCCAGAATATTATGTGTTATTTAAAGTGCGGTTGCTTTTACCGGCGTTTTTAATGTTCAAAAACGTGGAAAAATTATTTGccc                                                 >  1:234240/1‑231 (MQ=255)                                                                                                                                                                                         aaGTAGTATGGATTAATATGTTGGTGCCCCCAACAGGACTTGAACCTGTGACTAATCGATTATGAGTCGACTGCTCTGACCGACTGAGCTATGGGGGCGGTGTGAGCGTAGAATTAAGGCGCCATTATAGGGAAATATTGGGGTAAAGTCCAGAATATTATGTGTTATTTAAAGTGCGGTTGCTTTTACCGGCGTTTTTAATGTTCAAAAACGTGGAAAAATTATTTGCCCTTTGAAAAGTCAGGTTGTTc                             <  1:358379/251‑1 (MQ=255)                                                                                                                                                                                             agtaTGGATTAATATGTTGGTGCCCCCAACAGGACTTGAACCTGTGACTAATCGATTATGAGTCGACTGCTCTGACCGACTGAGCTa                                                                                                                                                                                             <  1:310222/87‑1 (MQ=255)                                                                                                                                                                                             agtaTGGATTAATATGTTGGTGCCCCCAACAGGACTTGAACCTGTGACTAATCGATTATGAGTCGACTGCTCTGACCGACTGAGCTATGGGGGCGGTGTGAGCGTAGAAt                                                                                                                                                                      >  1:691150/1‑110 (MQ=255)                                                                                                                                                                                                  ggATTAATATGTTGGTGCCCCCAACAGGACTTGAACCTGTGACTAATCGATTATGAGTCGACTGCTCTGACCGACTGAGCTATGGGGGCGGTGTGAGCGTAGAATTAAGGCGCCATTATAGGGAAATATTGGGGTAAAGTCCAGAATATTAtgt                                                                                                                     <  1:33021/154‑1 (MQ=255)                                                                                                                                                                                                  ggATTAATATGTTGGTGCCCCCAACAGGACTTGAACCTGTGACTAATCGATTATGAGTCGACTGCTCTGACCGACTGAGCTATGGGGGCGGTGTGAGCGTAGAATTAAGGCGCCATTATAGGGAAATATTGGGGTAAAGTCCAGAATATTATGTGTTATTTAAAGTGCGGTTGCTTTTACCGGCGTTTTTAATGTTCAAAAACGTGGAAAAATTATTTGCCCTTTGAAAAGTCAGGTTGTTc                             <  1:518454/242‑1 (MQ=255)                                                                                                                                                                                                       aaTATGTTGGTGCCCCCAACAGGACTTGAACCTGTGACTAATCGATTATGAGTCGACTGCTCTGACCGACTGAGCTATGGGGGCGGTGTGAGCGTAGAATTAAGGCGCCATTATAGGGAAATATTGGGGTAAAGTCCAGAATATTATGTGTTATTTAAAGTGCGGTTGCTTTTACCGGCGTTTTTAATGTTCAAAAACGTGGAAAAATTATTTGCCCTTTGAAAAGTCAGGTTGTTCCTTGTGATTTTAGc               <  1:285904/251‑1 (MQ=255)                                                                                                                                                                                                        atatGTTGGTGCCCCCAACAGGACTTGAACCTGTGACTAATCGATTATGAGTCGACTGCTCTGACCGACTGAGCTATGGGGGCGGTGTGAGCGTAGAATTAAGGCGCCATTATAGGGAAATATTGGGGTAAAGTCCAGAATATTATGTGTTATTTAAAGTGCGGTTGCTTTTACCGGCGTTTTTAATGTTCAAAAACGTGGAAAAATTATTTGCCCTTTGAAAAGTCAGGTTGTTCCTTGTGATTTTAg                >  1:543590/1‑249 (MQ=255)                                                                                                                                                                                                                 tGCCCCCAACAGGACTTGAACCTGTGACTAATCGATTATGAGTCGACTGCTCTGACCGACTGAGCTATGGGGGCGGTGTGAGCGTAGAATTAAGGCGCCATTATAGGGAAATATTGGGGTAAAGTCCAGAATATTATGTGTTATTTAAAGTGCGGTTGCTTTTACCGGCGTTTTTAATGTTCAAAAACGTGGAAAAATTATTTGCCCTTTGAAAAGTCAGGTTGTTCCTTGTGATTTTAGCTGATTAGCtt     <  1:608237/251‑1 (MQ=255)                                                                                                                                                                                                                  gCCCCCAACAGGACTTGAACCTGTGACTAATCGATTATGAGTCGACTGCTCTGACCGACTGAGCTATGGGGGCGGTGTGAGCGTAGAATTAAGGCGCCATTATAGGGAAATATTGGGGTAAAGTCCAGAATATTATGTGTTATTTAAAGTGCGGTTGCTTTTACCGGCGTTTTTAATGTTCAAAAACGTGGAAAAATTATTTGCCCTTTGAAAAGTCAGGTTGTTCCTTGTGATTTTAGCTGATTAGCttt    >  1:675726/1‑251 (MQ=255)                                                                                                                                                                                                                   cccccAACAGGACTTGAACCTGTGACTAATCGATTATGAGTCGACTGCTCTGACCGACTGAGCTATGGGGGCGGTGTGAGCGTAGAATTAAgg                                                                                                                                                                 <  1:677948/93‑1 (MQ=255)                                                                                                                                                                                                                    ccccAACAGGACTTGAACCTGTGACTAATCGATTATGAGTCGACTGCTCTGACCGACTGAGCTATGGGGGCGGTGTGAGCGTAGAATTAAGGCGCCATTATAGGGAAATATTGGGGTAAAGTCCAGAATATTATGTGTTATTTAAAGTGCGGTTGCTTTTACCGGCGTTTTTAATGTTCAAAAACGTGGAAAAATTATTTGCCCTTTGAAAAGTCAGGTTGTTCCTTGTGATTTTAGCTGATTAGCTTTtg  >  1:380427/1‑251 (MQ=255)                                                                                                                                                                                                                       cAACAGGACTTGAACCTGTGACTAATCGATTATGAGTCGACTGCTCTGACCGACTGAGCTATGGGGGCGGTGTGAGCGTAGAATTAAGGCGCCATTATAGGGAAATATTGGGGTAAAGTCCAGAATATTATGTGTTAttt                                                                                                              >  1:810445/1‑140 (MQ=255)                                                                                                                                                                                                                        aaCAGGACTTGAACCTGTGACTAATCGATTATGAGTCGACTGCTCTGACCGACTGAGCTATGGGGGCGGTGTGAGCGTAGAATTAAGGCGCCATTATAGGGAAATATTGGGGTAAAGTCCAGAATATTATGTGTTATTTaa                                                                                                            <  1:294625/141‑1 (MQ=255)                                                                                                                                                                                                                              aCTTGAACCTGTGACTAATCGATTATGAGTCGACTGCTCTGACCGACTGAGCTATGGGGGCGGTGTGAGCGTAGAATTAAGGCGCCATTATAGGGAAATATTGGGGTAAAGTCCAGAat                                                                                                                            <  1:253778/119‑1 (MQ=255)                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                       AAACCAACTCTTTTGTCTACTATGCCTATTTTTAGTACCTAGAAAATCACTAAATTCCCCTGATAGGGTACTATATCAGGTACTAAAGGTAAAACTAAAATGAATCAAAAAAAGCATAATACACTATGGAAATGATTAAATATGTTAATTTATAAGTGGTTTTGGTTTGACTTGAATCAGAGTGAAGTAGTATGGATTAATATGTTGGTGCCCCCAACAGGACTTGAACCTATGACTAATCGATTATGAGTCGACTGCTCTGACCGACTGAGCTATGGGGGCGGTGTGAGCGTAGAATTAAGGCGCCATTATAGGGAAATATTGGGGTAAAGTCCAGAATATTATGTGTTATTTAAAGTGCGGTTGCTTTTACCGGCGTTTTTAATGTTCAAAAACGTGGAAAAATTATTTGCCCTTTGAAAAGTCAGGTTGTTCCTTGTGATTTTAGCTGATTAGCTTTTG  >  1/850959‑851420

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑