Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	778,056	T→A	T560T (ACT→ACA)	unknown →	Coupling protein VirD4, ATPase required for T‑DNA transfer

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	778,056	0	T	A	100.0%	176.7 / NA	50	T560T (ACT→ACA)	unknown	Coupling protein VirD4, ATPase required for T‑DNA transfer
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (25/25);  total (25/25)

GTAGATAAATTAAGCCATAGTCGCGGAAAAACTTCCAGTCACAGTATTACCGACCACGGACGACCGTTGCTTTTACCGCAGGAATTTAGACTAATAGGAGAATTTAAAGAAGTGGTGATGTTAAATAACAGCAAACCGATTATGTGTAATAAGGCGGTGTATTACAACGACCCTTATTTCATCGATAAATTGAAAGCGGTTTCCCCACAACTCCGCGCATTAGGCAAGAAATTACCGACTAAAGCGCAACTGGAAAAAGCCATGTTCAGTGGTGAGCTTTCCGCCCAATCTATTTAATCCACAAAAACTGTGAATAACCAGGAGGATAATATGAAAAAATTTTTAGTTATGAATACGTTAATCCCTGTTTGCTTTTTAAGCGCGTGCAGCTCGTCAACGCCGGAACCGCCACAAATCGATG  >  1/777817‑778237                                                                                                                                                                                                                                                |                                                                                                                                                                                      gtaaaTAAATTAAGCCATAGTCGAGGTAAAACATCCAGTCACAGTATTACCGACCATGGGCGACCATTACTTTTACCGCAGGAGTTTAGATTAATCGGCGAATTTAAAGAAGTGGTGATGTTAAATAACAGCAAACCGATTATATGTAATAAGGCAGTGTATTACAACGATCCTTATTTTATAGACAAACTCAAAGCGGTATCTCCGCAATTGCGAGCATTAGGTAAAAAATTACCGACAAAAgaacaatt                                                                                                                                                                            >  1:549570/5‑244 (MQ=255)               ccATAGTCGAGGTAAAACATCCAGTCACAGTATTACCGACCATGGGCGACCATTACTTTTACCGCAGGAGTTTAGATTAATCGGCGAATTTAAAGAAGTGGTGATGTTAAATAACAGCAAACCGATTATATGTAATAAGGCAGTGTATTACAACGATCCTTATTTTATAGACAAACTCAAAGCGGTATCTCCGCAATTGCGAGCATTAGGTAAAAAATTACCGACAAAAGAACAATTGGAAAAAGCTATGt                                                                                                                                                              <  1:94956/251‑1 (MQ=255)               ccATAGTCGAGGTAAAACATCCAGTCACAGTATTACCGACCATGGGCGACCATTACTTTTACCGCAGGAGTTTAGATTAATCGGCGAATTTAAAGAAGTGGTGATGTTAAATAACAGCAAACCGATTATATGTAATAAGGCAGTGTATTACAACGATCCTTATTTTATAGACAAACTCAAAGCGGTATCTCCGCAATTGCGAGCATTAGGTAAAAAATTACCGACAAAAGAACAATTGGAAAAAGCTATGt                                                                                                                                                              >  1:653968/1‑251 (MQ=255)                      cgaggtaaaaCATCCAGTCACAGTATTACCGACCATGGGCGACCATTACTTTTACCGCAGGAGTTTAGATTAATCGGCGAATTTAAAGAAGTGGTGATGTTAAATAACAGCAAACCGATTATATGTAATAAGGCAGTGTATTACAACGATCCTTATTTTATAGACAAACTCAAAGCGGTATCTCCGCAATTGCGAGCATTAGGTAAAAAATTACCGACAAAAGAACAATTGGAAAAAGCTATGTTCAgtgg                                                                                                                                                       <  1:769097/245‑1 (MQ=255)                      cgaggtaaaaCATCCAGTCACAGTATTACCGACCATGGGCGACCATTACTTTTACCGCAGGAGTTTAGATTAATCGGCGAATTTAAAGAAGTGGTGATGTTAAATAACAGCAAACCGATTATATGTAATAAGGCAGTGTATTACAACGATCCTTATTTTATAGACAAACTCAAAGCGGTATCTCCGCAATTGCGAGCATTAGGTAAAAAATTACCGACAAAAGAACAATTGGAAAAAGCTATGTTCAgtgg                                                                                                                                                       <  1:330234/245‑1 (MQ=255)                                            gTATTACCGACCATGGGCGACCATTACTTTTACCGCAGGAGTTTAGATTAATCGGCGAATTTAAAGAAGTGGTGATGTTAAATAACAGCAAACCGATTATATGTAATAAGGCAGTGTATTACAACGATCCTTATTTTATAGACAAACTCAAAGCGGTATCTCCGCAATTGCGAGCATTAGGTAAAAAATTACCGACAAAAGAACAATTGGAAAAAGCTATGTTCAGTGGCGAGCTGTCAGTCCAATCAAtt                                                                                                                                 >  1:504881/1‑251 (MQ=255)                                                             cgacCATTACTTTTACCGCAGGAGTTTAGATTAATCGGCGAATTTAAAGAAGTGGTGATGTTAAATAACAGCAAACCGATTATATGTAATAAGGCAGTGTATTACAACGATCCTTATTTTATAGACAAACTCAAAGCGGTATCTCCGCAATTGCGAGCATTAGGTAAAAAATTACCGACAAAAGAACAATTGGAAAAAGCTATGTTCAGTGGCGAGCTGTCAGTCCAATCAATTTAATCCACAAAAACtg                                                                                                                 <  1:47410/250‑1 (MQ=255)                                                                     acTTTTACCGCAGGAGTTTAGATTAATCGGCGAATTTAAAGAAGTGGTGATGTTAAATAACAGCAAACCGATTATATGTAATAAGGCAGTGTATTACAACGATCCTTATTTTATAGACAAACTCAAAGCGGTATCTCCGCAATTGCGAGCATTAGGTAAAAAATTACCGACAAAAgaac                                                                                                                                                                                >  1:35478/2‑176 (MQ=255)                                                                     acTTTTACCGCAGGAGTTTAGATTAATCGGCGAATTTAAAGAAGTGGTGATGTTAAATAACAGCAAACCGATTATATGTAATAAGGCAGTGTATTACAACGATCCTTATTTTATAGACAAACTCAAAGCGGTATCTCCGCAATTGCGAGCATTAGGTAAAAAATTACCGACAAAAGAACAATTGGAAAAAGCTATGTTCAGTGGCGAGCTGTCAGTCCAATCAATTTAATCCACAAAAACTGTGAATAAc                                                                                                         >  1:134825/2‑250 (MQ=255)                                                                      cTTTTACCGCAGGAGTTTAGATTAATCGGCGAATTTAAAGAAGTGGTGATGTTAAATAACAGCAAACCGATTATATGTAATAAGGCAGTGTATTACAACGATCCTTATTTTATAGACAAACTCAAAGCGGTATCTCCGCAATTGCGAGCATTAGGTAAAAAATTACCGACAAAAGAACAATTGGAAAAAGCTATGTTCAGTGGCGAGCTGTCAGTCCAATCAATTTAATCCACAAAAACTGTGAATAACCa                                                                                                       <  1:244723/251‑1 (MQ=255)                                                                                  gagtttAGATTAATCGGCGAATTTAAAGAAGTGGTGATGTTAAATAACAGCAAACCGATTATATGTAATAAGGCAGTGTATTACAACGATCCTTATTTTATAGACAAACTCAAAGCGGTATCTCCGCAATTGCGAGCATTAGGTAAAAAATTACCGACAAAAGAACAATTGGAAAAAGCTATGTTCAGTGGCGAGCTGTCAGTCCAATCAATTTAATCCACAAAAACTGTGAATAACCAGGAGGataa                                                                                              <  1:556097/245‑1 (MQ=255)                                                                                  gagtttAGATTAATCGGCGAATTTAAAGAAGTGGTGATGTTAAATAACAGCAAACCGATTATATGTAATAAGGCAGTGTATTACAACGATCCTTATTTTATAGACAAACTCAAAGCGGTATCTCCGCAATTGCGAGCATTAGGTAAAAAATTACCGACAAAAGAACAATTGGAAAAAGCTATGTTCAGTGGCGAGCTGTCAGTCCAATCAATTTAATCCACAAAAACTGTGAATAACCAGGAGGataa                                                                                              <  1:470740/245‑1 (MQ=255)                                                                                   agtttAGATTAATCGGCGAATTTAAAGAAGTGGTGATGTTAAATAACAGCAAACCGATTATATGTAATAAGGCAGTGTATTACAACGATCCTTATTTTATAGACAAACTCAAAGCGGTATCTCCGCAATTGCGAGCATTAGGTAAAAAATTACCGACAAAAGAACAATTGGAAAAAGCTATGTTCAGTGGCGAGCTGTCAGTCCAATCAATTTAATCCACAAAAACTGTGAATAACCAGGAGGATAATATg                                                                                          >  1:650002/3‑251 (MQ=255)                                                                                                     aaTTTAAAGAAGTGGTGATGTTAAATAACAGCAAACCGATTATATGTAATAAGGCAGTGTATTACAACGATCCTTATTTTATAGACAAACTCAAAGCGGTATCTCCGCAATTGCGAGCATTAGGTAAAAAATTACCGACAAAAGAACAATTGGAAAAAGCTATGTTCAGTGGCGAGCtgtcagtcc                                                                                                                                         >  1:801957/1‑178 (MQ=255)                                                                                                      aTTTAAAGAAGTGGTGATGTTAAATAACAGCAAACCGATTATGTGTAATAAGGCAGTGTATTACAACGATCCTTATTTTATAGACAAACTCAAAGCGGTATCTCCGCAATTGCGGGCATTAGGTAAAAAATTACCGACAAAAGAACAATTGGAAAAAGCTATGTTCAGTGGCGAGCTGTCAGTCCAATCAATTTAATCCACAAAAACTGTGAATAAcc                                                                                                        >  1:768856/1‑218 (MQ=255)                                                                                                                    tgATGTTAAATAACAGCAAACCGATTATATGTAATAAGGCAGTGTATTACAACGATCCTTATTTTATAGACAAACTCAAAGCGGTATCTCCGCAATTGCGAGCATTAGGTAAAAAATTACCGACAAAAGAACAATTGGAAAAAGCTATGTTCAGTGGCGAGCTGTCAGTCCAATCAATTTAATCCACAAAAACTGTGAATAACCAGGAGGATAATATGAAAAAAATTTTACTTATGAATATGTTAAtacc                                                          <  1:403990/250‑4 (MQ=255)                                                                                                                    tgATGTTAAATAACAGCAAACCGATTATATGTAATAAGGCAGTGTATTACAACGATCCTTATTTTATAGACAAACTCAAAGCGGTATCTCCGCAATTGCGAGCATTAGGTAAAAAATTACCGACAAAAGAACAATTGGAAAAAGCTATGTTCAGTGGCGAGCTGTCAGTCCAATCAATTTAATCCACAAAAACTGTGAATAACCAGGAGGATAATATGAAAAAAATTTTACTTATGAATATGTTAAtacc                                                          <  1:434082/250‑4 (MQ=255)                                                                                                                      aTGTTAAATAACAGCAAACCGATTATATGTAATAAGGCAGTGTATTACAACGATCCTTATTTTATAGACAAACTCAAAGCGGTATCTCCGCAATTGCGAGCATTAGGTAAAAAATTACCGACAAAAGAACAATTGGAAAAAGCTATGTTCAGTGGCGAGCTGTCAGTCCAATCAATTTAATcc                                                                                                                           >  1:161566/1‑183 (MQ=255)                                                                                                                           aaaTAACAGCAAACCGATTATATGTAATAAGGCAGTGTATTACAACGATCCTTATTTTATAGACAAACTCAAAGCGGTATCTCCGCAATTGCGAGCATTAGGTAAAAAATTACCGACAAAAGAACAATTGGAAAAAGCTATGTTCAGTGGCGAGCTGTCAGTCCAATCAATTTAATCCACAAAAACTGTGAATAAcc                                                                                                        <  1:544670/197‑1 (MQ=255)                                                                                                                           aaaTAACAGCAAACCGATTATATGTAATAAGGCAGTGTATTACAACGATCCTTATTTTATAGACAAACTCAAAGCGGTATCTCCGCAATTGCGAGCATTAGGTAAAAAATTACCGACAAAAGAACAATTGGAAAAAGCTATGTTCAGTGGCGAGCTGTCAGTCCAATCAATTTAATCCACAAAAACTGTGAATAACCAGGAGGATAATATGAAAAAAATTTTACTTATg                                                                        <  1:136376/229‑1 (MQ=255)                                                                                                                                    cAAACCGATTATATGTAATAAGGCAGTGTATTACAACGATCCTTATTTTATAGACAAACTCAAAGCGGTATCTCCGCAATTGCGAGCATTAGGTAAAAAATTACCGACAAAAGAACAATTGGAAAAAGCTATGTTCAGTGGCGAGCTGTCAGTCCAATCAATTTAATCCACAAAAACTGTGAATAACCAGGAGGATAATATGAAAAAAATTTTACTTATGAATATGTTAATACCTGTTTGCTTTTTAAg                                           <  1:41541/249‑1 (MQ=255)                                                                                                                                        ccGATTATATGTAATAAGGCAGTGTATTACAACGATCCTTATTTTATAGACAAACTCAAAGCGGTATCTCCGCAATTGCGAGCATTAGGTAAAAAATTACCGACAAAAGAACAATTGGAAAAAGCTATGTTCAGTGGCGAGCTGTCAGTCCAATCAATTTAATCCACAAAAACTGTGAATAACCAGGAGGATAATATGAAAAAAATTTTACTTATGAATATGTTAAtac                                                           >  1:636159/1‑227 (MQ=255)                                                                                                                                              atatgtAATAAGGCAGTGTATTACAACGATCCTTATTTTATAGACAAACTCAAAGCGGTATCTCCGCAATTGCGAGCATTAGGTAAAAAATTACCGACAAAAGAACAATTGGAAAAAGCTATGTTCAGTGGCGAGCTGTCAGTCCAATCAATTTAATCCACAAAAACTGTGAATAACCAGGAGGATAatat                                                                                           >  1:29581/4‑191 (MQ=255)                                                                                                                                                atgtAATAAGGCAGTGTATTACAACGATCCTTATTTTATAGACAAACTCAAAGCGGTATCTCCGCAATTGCGAGCATTAGGTAAAAAATTACCGACAAAAGAACAATTGGAAAAAGCTATGTTCAGTGGCGAGCTGTCAGTCCAATCAATTTAATCCACAAAAACTGTGAATAACCAGGAGGATAATATGAAAAAAATTTTACTTATGAATATGTTAATACCTGTTTGCTTTTTAAGCGCGTGCAGCTCGt                             >  1:412919/2‑251 (MQ=255)                                                                                                                                                   taataaGGCAGTGTATTACAACGATCCTTATTTTATAGACAAACTCAAAGCGGTATCTCCGCAATTGCGAGCATTAGGTAAAAAATTACCGACAAAAGAACAATTGGAAAAAGCTATGTTCAGTGGCGAGCTGTCAGTCCAATCAATTTAATCCACAAAAACTGTGAATAACCAGGAGGATAatat                                                                                           >  1:98836/1‑186 (MQ=255)                                                                                                                                                   taataaGGCAGTGTATTACAACGATCCTTATTTTATAGACAAACTCAAAGCGGTATCTCCGCAATTGCGAGCATTAGGTAAAAAATTACCGACAAAAGAACAATTGGAAAAAGCTATGTTCAGTGGCGAGCTGTCAGTCCAATCAATTTAATCCACAAAAACTGTGAATAACCAGGAGGATAATATGAAAAAAATTTTACTTATGAATATGTTAAtacc                                                          <  1:757953/219‑4 (MQ=255)                                                                                                                                                   taataaGGCAGTGTATTACAACGATCCTTATTTTATAGACAAACTCAAAGCGGTATCTCCGCAATTGCGAGCATTAGGTAAAAAATTACCGACAAAAGAACAATTGGAAAAAGCTATGTTCAGTGGCGAGCTGTCAGTCCAATCAATTTAATCCACAAAAACTGTGAATAACCAGGAGGATAATATGAAAAAAATTTTACTTATGAATATGTTAATACCTGTTTGCTTTTTAAGCGCGTGCAGCTCGTCa                           >  1:165664/1‑250 (MQ=255)                                                                                                                                                   taataaGGCAGTGTATTACAACGATCCTTATTTTATAGACAAACTCAAAGCGGTATCTCCGCAATTGCGAGCATTAGGTAAAAAATTACCGACAAAAGAACAATTGGAAAAAGCTATGTTCAGTGGCGAGCTGTCAGTCCAATCAATTTAATCCACAAAAACTGTGAATAACCAGGAGGATAATATGAAAAAAATTTTACTTATGAATATGTTAATACCTGTTTGCTTTTTAAGCGCGTGCAGCTCGTCa                           >  1:565257/1‑250 (MQ=255)                                                                                                                                                   taataaGGCAGTGTATTACAACGATCCTTATTTTATAGACAAACTCAAAGCGGTATCTCCGCAATTGCGAGCATTAGGTAAAAAATTACCGACAAAAGAACAATTGGAAAAAGCTATGATCAGTGGCGAGCTGTCAGTCCAATCAATTTAATCCACAAAAACTGTGAATAACCaggagg                                                                                                  <  1:498361/179‑1 (MQ=255)                                                                                                                                                    aataaGGCAGTGTATTACAACGATCCTTATTTTATAGACAAACTCAAAGCGGTATCTCCGCAATTGCGAGCATTAGGTAAAAAATTACCGACAAAAGAACAATTGGAAAAAGCTATGTTCAGTGGCGAGCTGTCAGTCCAATCAATTTAATCCACAAAAACTGTGAATAACCAGGAGGATAATATGAAAAAAATTTTACTTATGAATATGTTAATACCTGTTTGCTTTTTAAGCGCGTGCAGCTCGTCacc                         <  1:646724/251‑3 (MQ=255)                                                                                                                                                        aGGCAGTGTATTACAACGATCCTTATTTTATAGACAAACTCAAAGCGGTATCTCCGCAATTGCGAGCATTAGGTAAAAAATTACCGACAAAAGAACAATTGGAAAAAGCTATGTTCAGTGGCGAGCTGTCAGTCCAATCAATTTAATCCACAAAAACTGTGAATAAc                                                                                                         <  1:166795/167‑1 (MQ=255)                                                                                                                                                               gtATTGCAACGATCCTTATTTTATAGACAAACTCAAAGCGGTATCTCCGCAATTGCGAGCATTAGGTAAAAAATTACCGACAAAAGAACAATTGGAAAAAGCTATGTTCAGTGGCGAGCTGTCAGTCCAATCAATTTAATCCACAAAAACTGTGAATAACCAGGAGGATAatat                                                                                           <  1:642961/174‑1 (MQ=255)                                                                                                                                                               gtATTACAACGATCCTTATTTTATAGACAAACTCAAAGCGGTATCTCCGCAATTGCGAGCATTAGGTAAAAAATTACCGACAAAAGAACAATTGGAAAAAGCTATGTTCAGTGGCGAGCTGTCAGTCCAATCAATTTAATCCACAAAAACTGTGAATAACCAggagga                                                                                                 <  1:509913/168‑1 (MQ=255)                                                                                                                                                               gtATTACAACGATCCTTATTTTATAGACAAACTCAAAGCGGTATCTCCGCAATTGCGAGCATTAGGTAAAAAATTACCGACAAAAGAACAATTGGAAAAAGCTATGTTCAGTGGCGAGCTGTCAGTCCAATCAATTTAATCCACAAAAACTGTGAATAACCAGGAGGatga                                                                                              >  1:434434/1‑169 (MQ=255)                                                                                                                                                               gtATTACAACGATCCTTATTTTATAGACAAACTCAAAGCGGTATCTCCGCAATTGCGAGCATTAGGTAAAAAATTACCGACAAAAGAACAATTGGAAAAAGCTATGTTCAGTGGCGAGCTGTCAGTCCAATCAATTTAATCCACAAAAACTGTGAATAACCAGGAGGATAATATGAAAAAAATTTTACTTATGAATATGTTAAtacc                                                          <  1:614625/207‑4 (MQ=255)                                                                                                                                                               gtATTACAACGATCCTTATTTTATAGACAAACTCAAAGCGGTATCTCCGCAATTGCGAGCATTAGGTAAAAAATTACCGACAAAAGAACAATTGGAAAAAGCTATGTTCAGTGGCGAGCTGTCAGTCCAATCAATTTAATCCACAAAAACTGTGAATAACCAGGAGGATAATATGAAAAAAATTTTACTTATGAATATGTTAAtac                                                           >  1:669767/1‑204 (MQ=255)                                                                                                                                                               gtATTACAACGATCCTTATTTTATAGACAAACTCAAAGCGGTATCTCCGCAATTGCGAGCATTAGGTAAAAAATTACCGACAAAAGAACAATTGGAAAAAGCTATGTTCAGTGGCGAGCTGTCAGTCCAATCAATTTAATCCACAAAAACTGTGAATAACCAGGAGGATAATATGAAAAAAATTTTACTTATGAATATGTTAAtac                                                           <  1:745828/206‑3 (MQ=255)                                                                                                                                                               gtATTACAACGATCCTTATTTTATAGACAAACTCAAAGCGGTATCTCCGCAATTGCGAGCATTAGGTAAAAAATTACCGACAAAAGAACAATTGGAAAAAGCTATGTTCAGTGGCGAGCTGTCAGTCCAATCAATTTAATCCACAAAAACTGTGAATAACCAGGAGGATAATATGAAAAAAATTTTACTTATGAATATGTTAATACCTGTTTGCTTTTTAAg                                           >  1:466134/1‑222 (MQ=255)                                                                                                                                                               gtATTACAACGATCCTTATTTTATAGACAAACTCAAAGCGGTATCTCCGCAATTGCGAGCATTAGGTAAAAAATTACCGACAAAAGAACAATTGGAAAAAGCTATGTTCAGTGGCGAGCTGTCAGTCCAATCAATTTAATCCACAAAAACTGTGAATAACCAGGAGGATAATATGAAAAAAATTTTACTTATGAATATGTTAATACCTGTTTGCTTTTTAAGCGCGTGCAGCTCGTCaccaccagaac                 >  1:643527/1‑238 (MQ=255)                                                                                                                                                               gtATTACAACGATCCTTATTTTATAGACAAACTCAAAGCGGTATCTCCGCAATTGCGAGCATTAGGTAAAAAATTACCGACAAAAGAACAATTGGAAAAAGCTATGTTCAGTGGCGAGCTGTCAGTCCAATCAATTTAATCCACAAAAACTGTGAATAACCAGGAGGATAATATGAAAAAAATTTTACTTATGAATATGTTAATACCTGTTTGCTTTTTAAGCGCGTGCAGCTCGTCac                          <  1:504059/239‑2 (MQ=255)                                                                                                                                                               gtATTACAACGATCCTTATTTTATAGACAAACTCAAAGCGGTATCTCCGCAATTGCGAGCATTAGGTAAAAAATTACCGACAAAAGAACAATTGGAAAAAGCTATGTTCAGTGGCGAGCTGTCAGTCCAATCAATTTAATCCACAAAAACTGTGAATAACCAGGAGGATAATATGAAAAAAATTTTACTTATGAATATGTTAATACCTGTTTGCTTTTTAAGCGCGTGCAGCTCGTCACCACCAGAACCg               >  1:659897/1‑250 (MQ=255)                                                                                                                                                                tATTACAACGATCCTTATTTTATAGACAAACTCAAAGCGGTATCTCCGCAATTGCGAGCATTAGGTAAAAAATTACCGACAAAAGAACAATTGGAAAAAGCTATGTTCAGTGGCGAGCTGTCAGTCCAATCAATTTAATCCACAAAAACTGTGAATAACCAGGAGGATAATATGAAAAAAATTTTACTTATGAATATGTTAATACCTGTTTGCTTTTTAAGCGCGTGCAGCTCGTCACCACCAGAACCGc              <  1:817973/250‑1 (MQ=255)                                                                                                                                                                 aTTACAACGATCCTTATTTTATAGACAAACTCAAAGCGGTATCTCCGCAATTGCGAGCATTAGGTAAAAAATTACCGACAAAAGAACAATTGGAAAAAGCTATGTTCAGTGGCGAGCTGTCAGTCCAATCAATTTAATCCACAAAAACTGTGAATAACCAGGAGGATAatat                                                                                           <  1:686158/172‑1 (MQ=255)                                                                                                                                                                 aTTACAACGATCCTTATTTTATAGACAAACTCAAAGCGGTATCTCCGCAATTGCGAGCATTAGGTAAAAAATTACCGACAAAAGAACAATTGGAAAAAGCTATGTTCAGTGGCGAGCTGTCAGTCCAATCAATTTAATCCACAAAAACTGTGAATAACCAGGAGGATAATATGAAAAAAATTTTACTTATGAATATGtt                                                                <  1:467659/199‑1 (MQ=255)                                                                                                                                                                     cAACGATCCTTATTTTATAGACAAACTCAAAGCGGTATCTCCGCAATTGCGAGCATTAGGTAAAAAATTACCGACAAAAGAACAATTGGAAAAAGCTATGTTCAGTGGCGAGCTGTCAGTCCAATCAATTTAATCCACAAAAACTGTGAATAACCAGGAGGATAatat                                                                                           >  1:766284/1‑168 (MQ=255)                                                                                                                                                                         gatccTTATTTTATAGACAAACTCAAAGCGGTATCTCCGCAATTGCGAGCATTAGGTAAAAAATTACCGACAAAAGAACAATTGGAAAAAGCTATGTTCAGTGGCGAGCTGTCAGTCCAATCAATTTAATCCACAAAAACTGTGAATAAcc                                                                                                        >  1:326548/4‑151 (MQ=255)                                                                                                                                                                          atccTTATTTTATAGACAAACTCAAAGCGGTATCTCCGCAATTGCGAGCATTAGGTAAAAAATTACCGACAAAAGAACAATTGGAAAAAGCTATGTTCAGTGGCGAGCTGTCAGTCCAATCAATTTAATCCACAAAAACTGTGAATAACCAGGAGGATAATATGAAAAAAATTTTACTTATGAATATGTTAAtacc                                                          <  1:777520/194‑4 (MQ=255)                                                                                                                                                                           tccTTATTTTATAGACAAACTCAAAGCGGTATCTCCGCAATTGCGAGCATTAGGTAAAAAATTACCGACAAAAGAACAATTGGAAAAAGCTATGTTCAGTGGCGAGCTGTCAGTCCAATCAATTTAATCCACAAAAACTGTGAATAACCAGGAGGATAATATGAAAAAAATTTTACTTATGAATATGTTAATACCTGTTTGCTTTTTAAGCGCGTGCAGCTCGTCACCACCAGAACCGCCGCAAATTGAta  >  1:274096/2‑250 (MQ=255)                                                                                                                                                                                                           ctccgcaattgcgagcATTAGGTAAAAAATTACCGACAAAAGAACAATTGGAAAAAGCTATGTTCAGTGGCGAGCTGTCAGTCCAATCAATTTAATCCACAAAAACTGTGAATAACCAGGAGGATAATATGAAAAAAATTTTACTTATg                                                                        >  1:326950/15‑149 (MQ=255)                                                                                                                                                                                                                                gtaaaaaaTTACCGACAAAAGAACAATTGGAAAAAGCTATGTTCAGTGGCGAGCTGTCAGTCCAATCAATTTAATCCACAAAAACTGTGAATAAcc                                                                                                        >  1:704669/6‑96 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                      GTAGATAAATTAAGCCATAGTCGCGGAAAAACTTCCAGTCACAGTATTACCGACCACGGACGACCGTTGCTTTTACCGCAGGAATTTAGACTAATAGGAGAATTTAAAGAAGTGGTGATGTTAAATAACAGCAAACCGATTATGTGTAATAAGGCGGTGTATTACAACGACCCTTATTTCATCGATAAATTGAAAGCGGTTTCCCCACAACTCCGCGCATTAGGCAAGAAATTACCGACTAAAGCGCAACTGGAAAAAGCCATGTTCAGTGGTGAGCTTTCCGCCCAATCTATTTAATCCACAAAAACTGTGAATAACCAGGAGGATAATATGAAAAAATTTTTAGTTATGAATACGTTAATCCCTGTTTGCTTTTTAAGCGCGTGCAGCTCGTCAACGCCGGAACCGCCACAAATCGATG  >  1/777817‑778237

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑