Predicted mutation
evidence seq id position mutation annotation gene description
RA 1 777,636 C→T G420G (GGC→GGT)  unknown → Coupling protein VirD4, ATPase required for T‑DNA transfer

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*1777,6360CT100.0% 283.5 / NA 84G420G (GGC→GGT) unknownCoupling protein VirD4, ATPase required for T‑DNA transfer
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (41/43);  total (41/43)

TACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGATGAACTACCGGAAACCAACCCGAAATTAAAATATCAGTTATTACTACTCATGGATGAGTTTACTGCGGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGCTATGGTTTACGCCTATTGATTATCTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGATAATATTGCCTGTAAAATTCTGTATGCTCCCGACAATCAGGAGGATGCAGAAGAATATTCTAAAGTGCTAGGTAATCGCACTGTAGATAAATTAAGCCATAGTCGCGGAAAAACTTCCAGTCACAGTATTACCGAC  >  1/777404‑777870
                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                          
tACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTg                                                                                                                                                                                                                          <  1:112942/251‑1 (MQ=255)
     gcTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATtat                                                                                                                                                                                                                     >  1:534837/1‑251 (MQ=255)
                 gAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATcaca                                                                                                                                                                                                         >  1:569374/1‑251 (MQ=255)
                    aaaaTCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAGTATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAgg                                                                                                                                                                                                      <  1:156221/251‑1 (MQ=255)
                         cACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAAc                                                                                                                                                                                                 <  1:22072/251‑1 (MQ=255)
                           cGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACtt                                                                                                                                                                                               >  1:261/1‑251 (MQ=255)
                           cGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACtt                                                                                                                                                                                               <  1:833786/251‑1 (MQ=255)
                             atttatttCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCg                                                                                                                                                                                             >  1:399776/1‑251 (MQ=255)
                                  tttCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTg                                                                                                                                                                                                                          >  1:653808/1‑217 (MQ=255)
                                     ccACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGAt                                                                                                                                                                                                                        >  1:51803/1‑216 (MQ=255)
                                                cAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATgg                                                                                                                                                                           <  1:840182/250‑1 (MQ=255)
                                                     gATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGGGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGcc                                                                                                                                                                                                                               >  1:66129/1‑193 (MQ=255)
                                                           aTCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGa                                                                                                                                                               <  1:21401/251‑1 (MQ=255)
                                                           aTCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGa                                                                                                                                                               <  1:751193/251‑1 (MQ=255)
                                                           aTCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGa                                                                                                                                                               <  1:556168/251‑1 (MQ=255)
                                                                ggCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGa                                                                                                                                                               <  1:264212/246‑1 (MQ=255)
                                                                       cAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGc                                                                                                                                                   <  1:321486/251‑1 (MQ=255)
                                                                           tCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTAt                                                                                                                                                >  1:520947/1‑250 (MQ=255)
                                                                                 aTCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGat                                                                                                                                         <  1:336499/251‑1 (MQ=255)
                                                                                    tGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGataat                                                                                                                                      <  1:605766/251‑1 (MQ=255)
                                                                                     gTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATtat                                                                                                                                                                                                                     >  1:96932/1‑171 (MQ=255)
                                                                                     gTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGa                                                                                                                                                               >  1:876365/1‑225 (MQ=255)
                                                                                      tttATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGATAatat                                                                                                                                    <  1:387344/251‑1 (MQ=255)
                                                                                             gCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGATAatatcgc                                                                                                                                 >  1:501454/1‑244 (MQ=255)
                                                                                                tGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATTTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATta                                                                                                                                                                                                                      <  1:193333/159‑1 (MQ=255)
                                                                                                    tATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGATAATATCGCCTGTAAAATTc                                                                                                                      <  1:290769/251‑1 (MQ=255)
                                                                                                             caaTATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCGGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATgg                                                                                                                                                                           <  1:561691/189‑1 (MQ=255)
                                                                                                             caaTATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCt                                                                                                                                                                                <  1:809451/184‑1 (MQ=255)
                                                                                                               atatTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGATAatat                                                                                                                                    <  1:411796/226‑1 (MQ=255)
                                                                                                               atatTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGATAATATCGCCTGTAAAATTCTATATGcacc                                                                                                            >  1:401896/1‑247 (MQ=255)
                                                                                                                    gacgaACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGat                                                                                                                                         >  1:275605/4‑216 (MQ=255)
                                                                                                                          cTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGcc                                                                                                                                                                                 >  1:245499/1‑170 (MQ=255)
                                                                                                                           tACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGATAATATCGCCTGTAAAATTCTATATGCACCCGACAACCaggag                                                                                               <  1:797333/251‑1 (MQ=255)
                                                                                                                           tACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGATAATATCGCCTGTAAAATTCTATATGCACCCGACAACCaggag                                                                                               <  1:74341/251‑1 (MQ=255)
                                                                                                                                gAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGATAATATCGCCTGTAAAATTCTATATGCACCCGACAACCAggaggacg                                                                                           >  1:581203/1‑248 (MQ=255)
                                                                                                                                  aaccaaccCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATgg                                                                                                                                                                           >  1:868593/1‑168 (MQ=255)
                                                                                                                                            acaTTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGc                                                                                                                                                                                                                                >  1:175587/3‑105 (MQ=255)
                                                                                                                                                 aagatatCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTc                                                                                                                                                                                              >  1:239604/4‑134 (MQ=255)
                                                                                                                                                  aaaTATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGATAATATCGCCTGTAAAATTCTATATGCACCCGACAACCAGGAGGACGCAGAAGAATATTCTAAAGt                                                                        >  1:324203/1‑251 (MQ=255)
                                                                                                                                                  aaaTATCAGTTAATACTACTAATGGATGAGTTTACTACCCTCGATATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCAATAAGCCTAATGGTTACGGTAAAGa                                                                                                                                                               <  1:278555/164‑1 (MQ=255)
                                                                                                                                                    atatCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGat                                                                                                                                         >  1:735325/1‑184 (MQ=255)
                                                                                                                                                        cAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGATAATATCGCCTGTAAAATTCTATATGCACCCGACAACCAGGAGGACGCAGAAGAATATTCTAAAGTGCTAgg                                                                  >  1:281098/1‑251 (MQ=255)
                                                                                                                                                          gTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGATAATATCGCCTGTAAAATTCTATATGCACCCGACAACCAGGAGGACGCAGAAGAATATTCTAAAGTGCTAGGTa                                                                >  1:509854/1‑251 (MQ=255)
                                                                                                                                                           ttattaCTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGATAATATCGCCTGTAAAATTCTATATGCACCCGACAACCAGGAGGACGCAGAAGAATATTCTAAAGTGCTAGGt                                                                 <  1:601565/249‑1 (MQ=255)
                                                                                                                                                           ttattaCTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGATAATATCGCCTGTAAAATTCTATATGCACCCGACAACCAGGAGGACGCAGAAGAATATTCTAAAGTGCTAGGTa                                                                <  1:264366/250‑1 (MQ=255)
                                                                                                                                                           ttattaCTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACGTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGATAATATCGCCTGTAAAATTCTATAGGCCCCCGACAACCAGGAGGACGCAGAAGAATATTCTAAAGTGCTAGGt                                                                 <  1:491335/249‑1 (MQ=255)
                                                                                                                                                            tattaCTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGat                                                                                                                                         <  1:75807/176‑1 (MQ=255)
                                                                                                                                                             attaCTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGATAATATCGCCTGTAAAATTCTATATGCACCCGACAACCAGGAGGACGCAGAAGAATATTCt                                                                             <  1:522706/235‑1 (MQ=255)
                                                                                                                                                               tactacTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGATAATATCGCCTGTAAAATTCTATATGCACCCGACAACCAGGAGGACGCAGAAGAATATTc                                                                              <  1:502246/232‑1 (MQ=255)
                                                                                                                                                                 ctactaATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAggg                                                                                                                                                                                                     >  1:860830/1‑111 (MQ=255)
                                                                                                                                                                 ctactaATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGa                                                                                                                                                               <  1:399882/149‑1 (MQ=255)
                                                                                                                                                                 ctactaATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGATAATATCGCCTGTAAAATTCTATATGCACCCGACAACCAGGAGGACGCAGAAGAATATTCTAAAGTGCTAGGTAATCGCAc                                                         <  1:153221/251‑1 (MQ=255)
                                                                                                                                                                 ctactaATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGATAATATCGCCTGTAAAATTCTATATGCACCCGACAACCAGGAGGACGCAGAAGAATATTCTAAAGTGCTAGGTAATCGCAc                                                         <  1:597058/251‑1 (MQ=255)
                                                                                                                                                                      aaTGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGATAATATCGCCTGTAAAATTCTATATGCACCCGACAACCAGGAGGACGCAGAAGAatat                                                                                >  1:118976/2‑223 (MQ=255)
                                                                                                                                                                           aTGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGATAATATCGCCTGTAAAATTCTATATGCACCCGACAACCAGGAGGACGCAGAAGAATATTCTAAAGTGCTAGGTAATCGCACTGtaaataaa                                               <  1:871369/251‑1 (MQ=255)
                                                                                                                                                                            tGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGATGCTAAAGCTATCCTGGATAATATCGCCTGTAAAATTCTATATGCACCCGACAACCAGGAGGACGCAGAAGAATATTCTAAAGTGCTAGGTAATCGCACTGTaaataaat                                              <  1:663503/251‑1 (MQ=255)
                                                                                                                                                                               gTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGATAATATCGCCTGTAAAATTCTATATGCACCCGACAACCAGGAGGACGCAGAAGAATATTCTAAAGTGCTAGGTAATCGCACTGTAAATAAATTaa                                           >  1:834656/1‑251 (MQ=255)
                                                                                                                                                                               gTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGATAATATCGCCTGTAAAATTCTATATGCACCCGACAACCAGGAGGACGCAGAAGAATATTCTAAAGTGCTAGGTAATCGCACTGTAAATAAATTaa                                           >  1:728261/1‑251 (MQ=255)
                                                                                                                                                                                           cggtATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGc                                                                                                                                                                                                    >  1:856675/1‑86 (MQ=255)
                                                                                                                                                                                           cggtATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGATAATATCGCCTGTAAAATTCTATATGCACCCGACAACCAGGAGGACGCAGAAGAATATTCTAAAGTGCTAGGTAATCGCACTGTaaataaat                                              <  1:617405/236‑1 (MQ=255)
                                                                                                                                                                                            ggtATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGATAATATCGCCTGTAAAATTCTATATGCACCCGACAACCAGGAGGACGCAGAAGAATATTCTAAAGTGCTAGGTAATCGCACTGTAAATAAATTAAGCCATAGTcgagg                              >  1:289812/1‑248 (MQ=255)
                                                                                                                                                                                             gtATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGa                                                                                                                                                               >  1:347171/1‑121 (MQ=255)
                                                                                                                                                                                             gtATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGat                                                                                                                                         <  1:826141/143‑1 (MQ=255)
                                                                                                                                                                                                 gTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGa                                                                                                                                                               >  1:169031/1‑117 (MQ=255)
                                                                                                                                                                                                 gTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGc                                                                                                                                                   >  1:847239/1‑129 (MQ=255)
                                                                                                                                                                                                 gTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGATAATATCGCCTGTAAAATTCTATATGCACCCGACAACCAGGAGGACGCAGAAGAATATTCTAAAGTGCTAGGt                                                                 <  1:649437/211‑1 (MQ=255)
                                                                                                                                                                                                 gTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGATAATATCGCCTGTAAAATTCTATATGCACCCGACAACCAGGAGGACGCAGAAGAATATTCTAAAGTGCTAGGTAATCg                                                            >  1:676668/1‑216 (MQ=255)
                                                                                                                                                                                                 gTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGATAATATCGCCTGTAAAATTCTATATGCACCCGACAACCAGGAGGACGCAGAAGAATATTCTAAAGTGCTAGGTAATCGCACTGTAAATAAATTAAGCCATAGTCGAGGtaaaa                         >  1:614476/1‑251 (MQ=255)
                                                                                                                                                                                                     aGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGATAATATCGCCTGTAAAATTCTATATGCACCCGACAACCAGGAGGACGCAGAAGAATATTCTAAAGTGCTAGGTAATCGCACTGTAAATAAATTAAGCCATAGTCGAGGTAAAAcat                      >  1:21669/1‑248 (MQ=255)
                                                                                                                                                                                                        aTTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGATAATATCGCCt                                                                                                                               <  1:890656/142‑1 (MQ=255)
                                                                                                                                                                                                         ttATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGATAATATCGCCTGTAAAATTCTATATGCACCCGACAACCAGGAGGACGCAGAAGAATATTCTAAAGTGCTAGGTAATCGCACTGTAAATAAATTAAGCCATAGTCGAGGTAAAACATCCAGt                 >  1:291938/1‑251 (MQ=255)
                                                                                                                                                                                                           aTCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGATAATATCGCCTGTAAAATTCTATATGCACCCGACAACCAGGAGGACGCAGAAGAATATTCTAAAGTGCTAGGTAATCGCAc                                                         <  1:519465/209‑1 (MQ=255)
                                                                                                                                                                                                           aTCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGATAATATCGCCTGTAAAATTCTATATGCACCCGACAACCAGGAGGACGCAGAAGAATATTCTAAAGTGCTAGGTAATCGCACTGTAAATAAATTAAGcc                                        <  1:52941/226‑1 (MQ=255)
                                                                                                                                                                                                            tCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGATAATATCGCCTGTAAAATTCTATATGCACCCGACAACCAGGAGGACGCAGAAGAATATTCTAAAGTGCTAGGTAATCGCACTGTAAATAAATTAAGCCATAGTcg                                 <  1:880184/232‑1 (MQ=255)
                                                                                                                                                                                                             cAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGATAATATCGCCTGTAAAATTCTATATGCACCCGACAACCAGGAGGACGCAGAAGAATATTCTAAAg                                                                         >  1:164694/1‑191 (MQ=255)
                                                                                                                                                                                                               aTAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGATAATATCGCCTGTAAAATTCTATATGCACCCGACAACCAggaggac                                                                                            >  1:843294/1‑169 (MQ=255)
                                                                                                                                                                                                                    tCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGATAATATCGCCTGTAAAATTCTATATGCACCCGACAACCAGGAGGACGCAGAAGAATATTCTAAAGTGCTAGGTAATCGCACTGtaaataaa                                               >  1:136271/1‑210 (MQ=255)
                                                                                                                                                                                                                    tCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGATAATATCGCCTGTAAAATTCTATATGCACCCGACAACCAGGAGGACGCAGAAGAATATTCTAAAGTGCTAGGTAATCGCACTGTAAATAAATTAAGCCATAGTCGAGGTAAAACATCCAGTCACAGTATTa       >  1:203545/1‑250 (MQ=255)
                                                                                                                                                                                                                    tCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGATAATATCGCCTGTAAAATTCTATATGCACCCGACAACCAGGAGGACGCAGAAGAATATTCTAAAGTGCTAGGTAATCGCACTGTAAATAAATTAAGCCATAGTCGAGGTAAAACATCCAGTCACAGTATTAc      <  1:701722/251‑1 (MQ=255)
                                                                                                                                                                                                                     cGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGATAATATCGCCTGTAAAATTCTATATGCACCCGACAACCAGGAGGACGCAGAAGAATATTCTAAAGTGCTAGGTAATCGCACTGTAAATAAATTAAGCCATAGTCGAGGTAAAACATCCAGTCACAGTATTAc      >  1:386526/1‑250 (MQ=255)
                                                                                                                                                                                                                       gTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGATAATATCGCCTGTAAAATTCTATATGCACCCGACAACCAGGAGGACGCAGAAGAATATTCTAAAGTGCTAGGTAATCGCACTGTAAATAAATTAAGCCATAGTCGAGGTAAAACATCCAGTCACAGTATTACCg    <  1:250654/250‑1 (MQ=255)
                                                                                                                                                                                                                        tAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGATAATATCGCCTGTAAAATTCTATATGCACCCGACAACCAGGAGGACGCAGAAGAATATTCTAAAGTGCTAGGTAATCGCACTGTAAATAAATTAAGCCATAGTCGAGGTAAAACATCCAGTCACAGTATTACCGAc  <  1:250490/251‑1 (MQ=255)
                                                                                                                                                                                                                           ggTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGATAATATCGCCTGTAAAATTCTATATGCACCCGACAACCAGGAGGACGCAGAAGAATATTCTaa                                                                           <  1:417322/175‑1 (MQ=255)
                                                                                                                                                                                                                            gTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGATAATATCGCCTGTAAAATTCTATATGCACCCGACAACCAGGAGGACGCagaag                                                                                     >  1:593935/1‑164 (MQ=255)
                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                          
TACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGATGAACTACCGGAAACCAACCCGAAATTAAAATATCAGTTATTACTACTCATGGATGAGTTTACTGCGGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGCTATGGTTTACGCCTATTGATTATCTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGATAATATTGCCTGTAAAATTCTGTATGCTCCCGACAATCAGGAGGATGCAGAAGAATATTCTAAAGTGCTAGGTAATCGCACTGTAGATAAATTAAGCCATAGTCGCGGAAAAACTTCCAGTCACAGTATTACCGAC  >  1/777404‑777870

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑