Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	777,522	T→C	D382D (GAT→GAC)	unknown →	Coupling protein VirD4, ATPase required for T‑DNA transfer

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	777,522	0	T	C	100.0%	224.7 / NA	66	D382D (GAT→GAC)	unknown	Coupling protein VirD4, ATPase required for T‑DNA transfer
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (26/40);  total (26/40)

TTATTTAATGGCGTGCGAATCCGGTAAAACAAAAAGTAGTATCGATTCAACTTTTACCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAACATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGATGAACTACCGGAAACCAACCCGAAATTAAAATATCAGTTATTACTACTCATGGATGAGTTTACTGCGGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGCTATGGTTTACGCCTATTGATTATCTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGATAATATTGCCTGTAAAATTCTGTATGCTCC  >  1/777282‑777764                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                   ttatttaATGGCGTGTGAATCCGGTAAGACAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCgg                                                                                                                                                                                                                                          >  1:100805/1‑251 (MQ=255)   atttaATGGCGTGTGAATCCGGTAAAACAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGaa                                                                                                                                                                                                                                        >  1:413599/1‑251 (MQ=255)      taATGGCGTGTGAATCCGGTAAAACAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAaacc                                                                                                                                                                                                                                     <  1:267617/251‑1 (MQ=255)               gtgAATCCGGTAAAACAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAAcccaaca                                                                                                                                                                                                                            >  1:249560/3‑247 (MQ=255)                     ccGGTAAAACAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTaaaa                                                                                                                                                                                                                      <  1:397684/251‑1 (MQ=255)                     ccGGTAAAACAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTaaaa                                                                                                                                                                                                                      <  1:2814/251‑1 (MQ=255)                         tAAAACAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAatat                                                                                                                                                                                                                   <  1:517120/250‑1 (MQ=255)                             aCAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGtt                                                                                                                                                                                                              >  1:459126/1‑251 (MQ=255)                             aCAAAAAGCAGTATCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGtt                                                                                                                                                                                                              <  1:453448/251‑1 (MQ=255)                                        taTCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATtactac                                                                                                                                                                                                      <  1:542122/248‑1 (MQ=255)                                          tCGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATg                                                                                                                                                                                                 <  1:238581/251‑1 (MQ=255)                                           cGATTCAACCTTTATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATgg                                                                                                                                                                                                >  1:596482/1‑251 (MQ=255)                                                 aacctttATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATtact                                                                                                                                                                                                        <  1:252071/233‑1 (MQ=255)                                                  acctttATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGtt                                                                                                                                                                                         >  1:195089/4‑251 (MQ=255)                                                  acctttATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGt                                                                                                                                                                                          >  1:48118/4‑250 (MQ=255)                                                    ctttATCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAAcccaacat                                                                                                                                                                                                                           >  1:164068/2‑210 (MQ=255)                                                      ttatcAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAat                                                                                                                                                                                                                     <  1:386040/215‑1 (MQ=255)                                                              ccGTTAACCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATg                                                                                                                                                                                             <  1:465579/235‑1 (MQ=255)                                                                   aaCCATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGt                                                                                                                                                                        <  1:570178/251‑1 (MQ=255)                                                                     ccATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTg                                                                                                                                                                      >  1:285079/1‑251 (MQ=255)                                                                     ccATTTTTCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTg                                                                                                                                                                      <  1:518562/251‑1 (MQ=255)                                                                        tttttCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGc                                                                                                                                                                   <  1:509584/251‑1 (MQ=255)                                                                          tttCAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCAt                                                                                                                                                                 <  1:632859/251‑1 (MQ=255)                                                                             cAAAATGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTAt                                                                                                                                                              <  1:608420/251‑1 (MQ=255)                                                                                  tGAAATTGTTGAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATa                                                                                                                                                         >  1:786649/1‑251 (MQ=255)                                                                                         gttgAATATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCAt                                                                                                                                                                 <  1:598106/236‑1 (MQ=255)                                                                                             aataTGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAgg                                                                                                                                               <  1:135605/247‑1 (MQ=255)                                                                                              ataTGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATtact                                                                                                                                                                                                        <  1:63564/190‑1 (MQ=255)                                                                                              ataTGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATg                                                                                                                                                                                                 >  1:240801/3‑199 (MQ=255)                                                                                                     aCTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGc                                                                                                                                      <  1:48175/251‑1 (MQ=255)                                                                                                        tCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCgg                                                                                                                                   >  1:844166/1‑251 (MQ=255)                                                                                                         cTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCggt                                                                                                                                  >  1:752925/1‑250 (MQ=255)                                                                                                                           tACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTg                                                                                                                <  1:112942/251‑1 (MQ=255)                                                                                                                                gcTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATtat                                                                                                           >  1:534837/1‑251 (MQ=255)                                                                                                                                            gAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGtta                                                                                                                                                                                                             <  1:358034/141‑1 (MQ=255)                                                                                                                                            gAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAAt                                                                                                                                                          <  1:313429/192‑1 (MQ=255)                                                                                                                                            gAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATcaca                                                                                               >  1:569374/1‑251 (MQ=255)                                                                                                                                               aaaaTCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAGTATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAgg                                                                                            <  1:156221/251‑1 (MQ=255)                                                                                                                                                    cACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAAc                                                                                       <  1:22072/251‑1 (MQ=255)                                                                                                                                                      cGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACtt                                                                                     <  1:833786/251‑1 (MQ=255)                                                                                                                                                      cGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACtt                                                                                     >  1:261/1‑251 (MQ=255)                                                                                                                                                        atttatttCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCg                                                                                   >  1:399776/1‑251 (MQ=255)                                                                                                                                                             tttCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTg                                                                                                                >  1:653808/1‑217 (MQ=255)                                                                                                                                                                ccACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGAt                                                                                                              >  1:51803/1‑216 (MQ=255)                                                                                                                                                                           cAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATgg                                                                 <  1:840182/250‑1 (MQ=255)                                                                                                                                                                                gATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGGGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGcc                                                                                                                     >  1:66129/1‑193 (MQ=255)                                                                                                                                                                                 aTTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCa                                                                                                                                                            <  1:691972/153‑1 (MQ=255)                                                                                                                                                                                      aTCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGa                                                     <  1:21401/251‑1 (MQ=255)                                                                                                                                                                                      aTCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGa                                                     <  1:751193/251‑1 (MQ=255)                                                                                                                                                                                      aTCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGa                                                     <  1:556168/251‑1 (MQ=255)                                                                                                                                                                                           ggCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGa                                                     <  1:264212/246‑1 (MQ=255)                                                                                                                                                                                                  cAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGc                                         <  1:321486/251‑1 (MQ=255)                                                                                                                                                                                                      tCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTAt                                      >  1:520947/1‑250 (MQ=255)                                                                                                                                                                                                            aTCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATg                                                                                                                                                                                                 >  1:280078/1‑89 (MQ=255)                                                                                                                                                                                                            aTCTGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGat                               <  1:336499/251‑1 (MQ=255)                                                                                                                                                                                                               tGTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGataat                            <  1:605766/251‑1 (MQ=255)                                                                                                                                                                                                                gTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATtat                                                                                                           >  1:96932/1‑171 (MQ=255)                                                                                                                                                                                                                gTTTATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGa                                                     >  1:876365/1‑225 (MQ=255)                                                                                                                                                                                                                 tttATGAGCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGATAatat                          <  1:387344/251‑1 (MQ=255)                                                                                                                                                                                                                        gCATGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGATAatatcgc                       >  1:501454/1‑244 (MQ=255)                                                                                                                                                                                                                           tGGTTATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATTTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATta                                                                                                            <  1:193333/159‑1 (MQ=255)                                                                                                                                                                                                                               tATTGCCAACAATATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGATAATATCGCCTGTAAAATTc            <  1:290769/251‑1 (MQ=255)                                                                                                                                                                                                                                        caaTATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCGGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATgg                                                                 <  1:561691/189‑1 (MQ=255)                                                                                                                                                                                                                                        caaTATTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCt                                                                      <  1:809451/184‑1 (MQ=255)                                                                                                                                                                                                                                          atatTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGATAatat                          <  1:411796/226‑1 (MQ=255)                                                                                                                                                                                                                                          atatTGACGAACTACCGGAAACCAACCCAACATTAAAATATCAGTTATTACTACTAATGGATGAGTTTACTGCCGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGTTATGGTTTACGCCTATTGATTATTTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGATAATATCGCCTGTAAAATTCTATATGcacc  >  1:401896/1‑247 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                   TTATTTAATGGCGTGCGAATCCGGTAAAACAAAAAGTAGTATCGATTCAACTTTTACCAGTCCGTTAACCATTTTTCAAAATGAAATTGTTGAACATGCGACTTCTGCCAGTGATTTTGATTTACGCGCTTTACGCAAGGAAAAAATCACGATTTATTTCCACATTTCGGCAAATGATTTAATCCTGGCTCCGCAAGTCGCTAATCTGTTTATGAGCATGGTTATTGCCAACAATATTGATGAACTACCGGAAACCAACCCGAAATTAAAATATCAGTTATTACTACTCATGGATGAGTTTACTGCGGTCGGTATGTTGAGCATTATCAATAAATCGGTAGGGTTTATCGCCGGCTATGGTTTACGCCTATTGATTATCTATCAATCACAAGGGCAACTTCGTGCCGATAAGCCTAATGGTTACGGTAAAGATGGTGCTAAAGCTATCCTGGATAATATTGCCTGTAAAATTCTGTATGCTCC  >  1/777282‑777764

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑