Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	774,772	T→C	V80A (GTA→GCA)	unknown →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	774,772	0	T	C	100.0%	257.5 / NA	78	V80A (GTA→GCA)	unknown	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (42/36);  total (42/36)

GAGATGAACGATAACAAAACCATTCAGCCGACGGATGACAAAACGGAAACCGATTATCAACCGGAAGTCAGCAAAATTGCCAAACGCGGTAAAAACCAAAATCTATTTATTTTTCTGATTATTGCACTGCTTGCCGTGGCGTTTTTGGGTTATTCCTTTTTGAATAAAAAGGATACGCAACCGGCACAGGTTAAGGAAAAAGAAGAATTCGGTACGACGGTGCGTTCAAAAACATTTACGGTACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATA  >  1/774531‑775016                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                     gagaTGAACGATAACAAAACCATTCAGCCGACGGATGACAAAACGGAAACCGATTATCAACCGGAAGTCAGCAAAATTGCCAAACGCGGTAAAAACCAAAATCTATTTATTTTTCTGATTATTGCACTGCTTGCCGTGGCGTTTTTGGGTTATTCCTTTTTGAATAAAAAGGATACGCAACCGGCACAGGTTAAGGAAAAAGAAGAATTCGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCgg                                                                                                                                                                                                                                              >  1:171320/1‑250 (MQ=255) gagaTGAACGATAACAAAACCATTCAGCCGACGGATGACAAAACGGAAACCGATTATCAACCGGAAGTCAGCAAAATTGCCAAACGCGGTAAAAACCAAAATCTATTTATTTTTCTGATTATTGCACTGCTTGCCGTGGCGTTTTTGGGTTATTCCTTTTTGAATAAAAAGGATACGCAACCGGCACAGGTTAAGGAAAAAGAAGAATTCGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCgg                                                                                                                                                                                                                                              >  1:151680/1‑250 (MQ=255)  agaTGAACGATAACAAAACCATTCAGCCGACGGATGACAAAACGGAAACCGATTATCAACCGGAAGTCAGCAAAATTGCCAAACGCGGTAAAAACCAAAATCTATTTATTTTTCTGATTATTGCACTGCTTGCCGTGGCGTTTTTGGGTTATTCCTTTTTGAATAAAAAGGATACGCAACCGGCACAGGTTAAGGAAAAAGAAGAATTCGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCg                                                                                                                                                                                                                                               <  1:104218/248‑1 (MQ=255)      gAACGATAACAAAACCATTCAGCCGACGGATGACAAAACGGAAACCGATTATCAACCGGAAGTCAGCAAAATTGCCAAACGCGGTAAAAACCAAAATCTATTTATTTTTCTGATTATTGCACTGCTTGCCGTGGCGTTTTTGGGTTATTCCTTTTTGAATAAAAAGGATACGCAACCGGCACAGGTTAAGGAAAAAGAAGAATTCGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGaaa                                                                                                                                                                                                                                        >  1:679643/1‑251 (MQ=255)       aaCGATAACAAAACCATTCAGCCGACGGATGACAAAACGGAAACCGATTATCAACCGGAAGTCAGCAAAATTGCCAAACGCGGTAAAAACCAAAATCTATTTATTTTTCTGATTATTGCACTGCTTGCCGTGGCGTTTTTGGGTTATTCCTTTTTGAATAAAAAGGATACGCAACCGGCACAGGTTAAGGAAAAAGAAGAATTCGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAAt                                                                                                                                                                                                                                       >  1:486234/1‑251 (MQ=255)               cAAAACCATTCAGCCGACGGGTGACAAAACGGAAACCGATTATCAACCGGAAGTCAGCAAAATTGCCAAACGCGGTAAAAACCAAAATCTATTTATTTTTCTGATTATTGCACTGCTTGCCGTGGCGTTTTTGGGTTATTCCTTTTTGAATAAAAAGGATACGCAACCGGCACAGGTTAAGGAAAAAGAAGAATTCGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGa                                                                                                                                                                                                                               <  1:328876/251‑1 (MQ=255)                                    tGACAAAACGGAAACCGATTATCAACCGGAAGTCAGCAAAATTGCCAAACGCGGTAAAAACCAAAATCTATTTATTTTTCTGATTATTGCACTGCTTGCCGTGGCGTTTTTGGGTTATTCCTTTTTGAATAAAAAGGATACGCAACCGGCACAGGTTAAGGAAAAAGAAGAATTCGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAAc                                                                                                                                                                                                          <  1:155866/251‑1 (MQ=255)                                            cGGAAACCGATTATCAACCGGAAGTCAGCAAAATTGCCAAACGCGGTAAAAACCAAAATCTATTTATTTTTCTGATTATTGCACTGCTTGCCGTGGCGTTTTTGGGTTATTCCTTTTTGAATAAAAAGGATACGCAACCGGCACAGGTTAAGGAAAAAGAAGAATTCGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCt                                                                                                                                                                                                  <  1:148901/251‑1 (MQ=255)                                                     aTTATCAACCGGAAGTCAGCAAAATTGCCAAACGCGGTAAAAACCAAAATCTATTTATTTTTCTGATTATTGCACTGCTTGCCGTGGCGTTTTTGGGTTATTCCTTTTTGAATAAAAAGGATACGCAACCGGCACAGGTTAAGGAAAAAGAAGAATTCGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGa                                                                                                                                                                                                                                          >  1:610980/1‑202 (MQ=255)                                                                                ccAAACGCGGTAAAAACCAAAATCTATTTATTTTTCTGATTATTGCACTGCTTGCCGTGGCGTTTTTGGGTTATTCCTTTTTGAATAAAAAGGATACGCAACCGGCACAGGTTAAGGAAAAAGAAGAATTCGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGAc                                                                                                                                                               <  1:822487/250‑1 (MQ=255)                                                                                       cgGTAAAAACCAAAATCTATTTATTTTTCTGATTATTGCACTGCTTGCCGTGGCGTTTTTGGGTTATTCCTTTTTGAATAAAAAGGATACGCAACCGGCACAGGTTAAGGAAAAAGAAGAATTCGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCg                                                                                                                                                        <  1:717041/250‑1 (MQ=255)                                                                                            aaaaCCAAAATCTATTTATTTTTCTGATTATTGCACTGCTTGCCGTGGCGTTTTTGGGTTATTCCTTTTTGAATAAAAAGGATACGCAACCGGCACAGGTTAAGGAAAAAGAAGAATTCGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGat                                                                                                                                                  <  1:648330/251‑1 (MQ=255)                                                                                            aaaaCCAAAATCTATTTATTTTTCTGATTATTGCACTGCTTGCCGTGGCGTTTTTGGGTTATTCCTTTTTGAATAAAAAGGATACGCAACCGGCACAGGTTAAGGAAAAAGAAGAATTCGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGat                                                                                                                                                  <  1:830719/251‑1 (MQ=255)                                                                                              aaCCAAAATCTATTTATTTTTCTGATTATTGCACTGCTTGCCGTGGCGTTTTTGGGTTATTCCTTTTTGAATAAAAAGGATACGCAACCGGCACAGGTTAAGGAAAAAGAAGAATTCGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGatat                                                                                                                                                >  1:178901/1‑251 (MQ=255)                                                                                              aaCCAAAATCTATTTATTTTTCTGATTATTGCACTGCTTGCCGTGGCGTTTTTGGGTTATTCCTTTTTGAATAAAAAGGATACGCAACCGGCACAGGTTAAGGAAAAAGAAGAATTCGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGa                                                                                                                                                   >  1:887704/1‑248 (MQ=255)                                                                                                 cAAAATCTATTTATTTTTCTGATTATTGCACTGCTTGCCGTGGCGTTTTTGGGTTATTCCTTTTTGAATAAAAAGGATACGCAACCGGCACAGGTTAAGGAAAAAGAAGAATTCGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGcc                                                                                                                                             <  1:61490/251‑1 (MQ=255)                                                                                                     aTCTATTTATTTTTCTGATTATTGCACTGCTTGCCGTGGCGTTTTTGGGTTATTCCTTTTTGAATAAAAAGGATACGCAACCGGCACAGGTTAAGGAAAAAGAAGAATTCGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATc                                                                                                                                          >  1:188800/1‑250 (MQ=255)                                                                                                     aTCTATTTATTTTTCTGATTATTGCACTGCTTGCCGTGGCGTTTTTGGGTTATTCCTTTTTGAATAAAAAGGATACGCAACCGGCACAGGTTAAGGAAAAAGAAGAATTCGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATc                                                                                                                                          >  1:853096/1‑250 (MQ=255)                                                                                                      tCTATTTATTTTTCTGATTATTGCACTGCTTGCCGTGGCGTTTTTGGGTTATTCCTTTTTGAATAAAAAGGATACGCAACCGGCACAGGTTAAGGAAAAAGAAGAATTCGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATCCg                                                                                                                                        >  1:53165/1‑251 (MQ=255)                                                                                                               ttttCTGATTATTGCACTGCTTGCCGTGGCGTTTTTGGGTTATTCCTTTTTGAATAAAAAGGATACGCAACCGGCACAGGTTAAGGAAAAAGAAGAATTCGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGtt                                                                                                                               <  1:571736/251‑1 (MQ=255)                                                                                                                     gATTATTGCACTGCTTGCCGTGGCGTTTTTGGGTTATTCCTTTTTGAATAAAAAGGATACGCAACCGGCACAGGTTAAGGAAAAAGAAGAATTCGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCa                                                                                                                         >  1:664779/1‑251 (MQ=255)                                                                                                                       ttattGCACTGCTTGCCGTGGCGTTTTTGGGTTATTCCTTTTTGAATAAAAAGGATACGCAACCGGCACAGGTTAAGGAAAAAGAAGAATTCGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGat                                                                                                                                                  >  1:518547/1‑224 (MQ=255)                                                                                                                       ttattGCACTGCTTGCCGTGGCGTTTTTGGGTTATTCCTTTTTGAATAAAAAGGATACGCAACCGGCACAGGTTAAGGAAAAAGAAGAATTCGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCa                                                                                                                                            <  1:815096/230‑1 (MQ=255)                                                                                                                       ttattGCACTGCTTGCCGTGGCGTTTTTGGGTTATTCCTTTTTGAATAAAAAGGATACGCAACCGGCACAGGTTAAGGAAAAAGAAGAATTCGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATCCGccc                                                                                                                                     <  1:660980/237‑1 (MQ=255)                                                                                                                       ttattGCACTGCTTGCCGTGGCGTTTTTGGGTTATTCCTTTTTGAATAAAAAGGATACGCAACCGGCACAGGTTAAGGAAAAAGAAGAATTCGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCaaa                                                                                                                       >  1:763379/1‑251 (MQ=255)                                                                                                                         attGCACTGCTTGCCGTGGCGTTTTTGGGTTATTCCTTTTTGAATAAAAAGGATACGCAACCGGCACAGGTTAAGGAAAAAGAAGAATTCGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAgg                                                                                                                     <  1:812013/251‑1 (MQ=255)                                                                                                                          ttGCTCTGCTTGCCGTGGCGTTTTTGGGTTATTCCTTTTTGAATAAAAAGGATACGCAACCGGCACAGGTTAAGGAAAAAGAAGAATTCGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAgg                                                                                                                                                          <  1:147723/213‑1 (MQ=255)                                                                                                                          ttGCACTGCTTGCCGTGGCGTTTTTGGGTTATTCCTTTTTGAATAAAAAGGATACGCAACCGGCACAGGTTAAGGAAAAAGAAGAATTCGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAg                                                                                                                                                                               <  1:241651/192‑1 (MQ=255)                                                                                                                               cTGCTTGCCGTGGCGTTTTTGGGTTATTCCTTTTTGAATAAAAAGGATACGCAACCGGCACAGGTTAAGGAAAAAGAAGAATTCGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGt                                                                                                                >  1:51334/1‑250 (MQ=255)                                                                                                                                 gCTTGCCGTGGCGTTTTTGGGTTATTCCTTTTTGAATAAAAAGGATACGCAACCGGCACAGGTTAAGGAAAAAGAAGAATTCGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATc                                                                                                                                                                                                                                      <  1:376748/130‑1 (MQ=255)                                                                                                                                 gCTTGCCGTGGCGTTTTTGGGTTATTCCTTTTTGAATAAAAAGGATACGCAACCGGCACAGGTTAAGGAAAAAGAAGAATTCGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGCTTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGtct                                                                                                              >  1:737058/1‑250 (MQ=255)                                                                                                                                          ggCGTTTTTGGGTTATTCCTTTTTGAATAAAAAGGATACGCAACCGGCACAGGTTAAGGAAAAAGAAGAATTCGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATc                                                                                                                          <  1:579407/229‑1 (MQ=255)                                                                                                                                             gTTTTTGGGTTATTCCTTTTTGAATAAAAAGGATACGCAACCGGCACAGGTTAAGGAAAAAGAAGAATTCGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCa                                                                                                                                            >  1:774243/1‑208 (MQ=255)                                                                                                                                              tttttGGGTTATTCCTTTTTGAATAAAAAGGATACGCAACCGGCACAGGTTAAGGAAAAAGAAGAATTCGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCg                                                                                                 <  1:845213/250‑1 (MQ=255)                                                                                                                                                   gggTTATTCCTTTTTGAATAAAAAGGATACGCAACCGGCACAGGTTAAGGAAAAAGAAGAATTCGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAgg                                                                                                                                                          <  1:159999/188‑1 (MQ=255)                                                                                                                                                   gggTTATTCCTTTTTGAATAAAAAGGATACGCAACCGGCACAGGTTAAGGAAAAAGAAGAATTCGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGa                                                                                                                                                   >  1:721287/1‑195 (MQ=255)                                                                                                                                                   gggTTATTCCTTTTTGAATAAAAAGGATACGCAACCGGCACAGGTTAAGGAAAAAGAAGAATTCGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCg                                                                                           >  1:204104/1‑251 (MQ=255)                                                                                                                                                        aTTCCTTTTTGAATAAAAAGGATACGCAACCGGCACAGGTTAAGGAAAAAGAAGAATTCGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGcc                                                                                                                                             >  1:878165/1‑196 (MQ=255)                                                                                                                                                          tCCTTTTTGAATAAAAAGGATACGCAACCGGCACAGGTTAAGGAAAAAGAAGAATTCGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCACAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGtt                                                                                    >  1:415863/1‑251 (MQ=255)                                                                                                                                                                ttGAATAAAAAGGATACGCAACCGGCACAGGTTAAGGAAAAAGAAGAATTCGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGc                                                                              <  1:161281/251‑1 (MQ=255)                                                                                                                                                                         aaGGATACGCAACCGGCACAGGTTAAGGAAAAAGAAGAATTCGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGtct                                                                                                              <  1:487867/210‑1 (MQ=255)                                                                                                                                                                           ggATACGCAACCGGCACAGGTTAAGGAAAAAGAAGAATTCGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACg                                                                                                                                 >  1:715761/1‑189 (MQ=255)                                                                                                                                                                            gATACGCAACCGGCACAGGTTAAGGAAAAAGAAGAATTCGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATg                                                                   <  1:770005/250‑1 (MQ=255)                                                                                                                                                                              tACGCAACCGGCACAGGTTAAGGAAAAAGAAGAATTCGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTc                                                                <  1:477622/251‑1 (MQ=255)                                                                                                                                                                                  cAACCGGCACAGGTTAAGGAAAAAGTAGAATTCGGTCCGACCGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAAt                                                                                                                                                                                                                       >  1:446029/1‑96 (MQ=255)                                                                                                                                                                                  cAACCGGCACAGGTTAAGGAAAAAGAAGAATTCGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTc                                                                >  1:106446/1‑247 (MQ=255)                                                                                                                                                                                   aaCCGGCACAGGTTAAGGAAAAAGAAGAATTCGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAAt                                                           <  1:494765/251‑1 (MQ=255)                                                                                                                                                                                       ggCACAGGTTAAGGAAAAAGAAGAATTCGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTAc                                                                                                      >  1:868652/1‑204 (MQ=255)                                                                                                                                                                                        gCACAGGTTAAGGAAAAAGAAGAATTCGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCGATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTAt                                                                                                   <  1:791342/206‑1 (MQ=255)                                                                                                                                                                                        gCACAGGTTAAGGAAAAAGAAGAATTCGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAgg                                                                                                                                                          >  1:811604/1‑151 (MQ=255)                                                                                                                                                                                         cacaGGTTAAGGAAAAAGAAGAATTCGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGa                                                      <  1:88770/250‑1 (MQ=255)                                                                                                                                                                                         cacaGGTTAAGGAAAAAGAAGAATTCGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGa                                                      <  1:467966/250‑1 (MQ=255)                                                                                                                                                                                            aGGTTAAGGAAAAAGAAGAATTCGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTAc                                                                                  >  1:660966/1‑219 (MQ=255)                                                                                                                                                                                             ggTTAAGGAAAAAGAAGAATTCGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCTGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGt                                                                                                                                >  1:232463/1‑172 (MQ=255)                                                                                                                                                                                             ggTTAAGGAAAAAGAAGAATTCGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTcc                                                                                                                                                                                    >  1:114553/1‑120 (MQ=255)                                                                                                                                                                                             ggTTAAGGAAAAAGAAGAATTCGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGt                                                                                                                                >  1:481830/1‑172 (MQ=255)                                                                                                                                                                                             ggTTAAGGAAAAAGAAGAATTCGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCggg                                                                                                         >  1:66401/1‑195 (MQ=255)                                                                                                                                                                                             ggTTAAGGAAAAAGAAGAATTCGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGa                                                      <  1:829485/246‑1 (MQ=255)                                                                                                                                                                                             ggTTAAGGAAAAAGAAGAATTCGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACaa                                                 >  1:712206/1‑251 (MQ=255)                                                                                                                                                                                             ggTTAAGGAAAAAGAAGAATTCGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACaa                                                 >  1:519502/1‑251 (MQ=255)                                                                                                                                                                                             ggTTAAGGAAAAAGAAGAATTCGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACaa                                                 >  1:537536/1‑251 (MQ=255)                                                                                                                                                                                             ggTTAAGGAAAAAGAAGAATTCGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACaa                                                 >  1:366638/1‑251 (MQ=255)                                                                                                                                                                                               ttAAGGAAAAAGAAGAATTCGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAAc                                                <  1:48056/250‑1 (MQ=255)                                                                                                                                                                                                    gAAAAAGAAGAATTCGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGc                                                                              >  1:46622/1‑215 (MQ=255)                                                                                                                                                                                                    gAAAAAGAAGAATTCGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGaca                                           <  1:117191/250‑1 (MQ=255)                                                                                                                                                                                                              aatgcGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGAAGAAATCACGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAAGCATAGAACGACAGAGGCGTTAGATATGCCAACCGCCCCACGTTTAATCAAAGGATTGTATCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGtaactaac                                <  1:592228/247‑1 (MQ=255)                                                                                                                                                                                                              aaTTCGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGtaactaac                                <  1:132038/251‑1 (MQ=255)                                                                                                                                                                                                               aTTCGGTACGACGGTGCGTTCAAAAACATTTACGGCCCCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGtaactaac                                <  1:537108/250‑1 (MQ=255)                                                                                                                                                                                                                  cGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTCCCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATg                                                                   <  1:217931/212‑1 (MQ=255)                                                                                                                                                                                                                  cGGTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTaaccaa                             >  1:892278/1‑250 (MQ=255)                                                                                                                                                                                                                    gTACGACGGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAaccaacca                          >  1:50322/1‑251 (MQ=255)                                                                                                                                                                                                                      acgacgGTGCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAaccac                         >  1:395665/1‑250 (MQ=255)                                                                                                                                                                                                                              gCGTTCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGaa                                                                                                                                                                   >  1:676221/1‑104 (MQ=255)                                                                                                                                                                                                                                 ttCAAAAACATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCaa             >  1:848378/1‑251 (MQ=255)                                                                                                                                                                                                                                  ttcaaaaCATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATa                                                    <  1:605665/208‑1 (MQ=255)                                                                                                                                                                                                                                         cATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGcc                                                                             >  1:529468/1‑179 (MQ=255)                                                                                                                                                                                                                                         cATTTACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGat     >  1:484210/1‑251 (MQ=255)                                                                                                                                                                                                                                            ttACGGCACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGatata  <  1:264328/251‑1 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                     GAGATGAACGATAACAAAACCATTCAGCCGACGGATGACAAAACGGAAACCGATTATCAACCGGAAGTCAGCAAAATTGCCAAACGCGGTAAAAACCAAAATCTATTTATTTTTCTGATTATTGCACTGCTTGCCGTGGCGTTTTTGGGTTATTCCTTTTTGAATAAAAAGGATACGCAACCGGCACAGGTTAAGGAAAAAGAAGAATTCGGTACGACGGTGCGTTCAAAAACATTTACGGTACCACCGGCAGAAATCCCGGCGATTTTAAATCCGGAACCACAACCAATGCCTACCGCAACCGCTCCAGTAGAAAATCATAGAACGACAGAGGCGTTAGATATGCCATCCGCCCCACGTTTAATCAAAGGATTGTCTCCGGGTACTATCGATGGCGAAACGTTACAAGCCGTTTCAGATGTTCAGAATACAGATACAACTGACACGGTAACTAACCAACCACCGGAACCGGCAAAAGGCGATATA  >  1/774531‑775016

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑