Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,416,644	+T	intergenic (+135/‑54)	unknown → / → unknown	Possible protease sohB (EC 3.4.21.‑)/Outer membrane protein A precursor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,416,643	1	.	T	100.0%	118.4 / NA	36	intergenic (+134/‑55)	unknown/unknown	Possible protease sohB (EC 3.4.21.‑)/Outer membrane protein A precursor
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (20/16);  total (20/16)

GTGAAGAAATCGTTATTACAAAAAGTCGGTAAACAGGCGGAAGAAAGTGCCGATAATATTGCTTTACGTTGGCTTAAGAAAAATGAAAGAACATTGATATAACATCCTATTTTTTTCGATTCTATTTATACAGATTGATTAAAAATAGAAAACTCGGATTTACTTTAATCCGTTTTGTAATTACCATACGGAGCTGAAATTGAAGTTGCACTATTGCTTTTTGAGCCTTCTTTTTA‑TATATCTATGGTTCCTTTGGATTGTTCTACTCACCTAAAAATAAGGTAAAACAGATGAAATTATCACATTTCTTATTATCTGCAATCGCAGCGGCAACTCTTGTTGCTTGTGGTAACTTAAGTAAAGTATCTAAAGAAGGTACTCCTGCCGAAGGTTCTGAATTAGTATGGCCTCAAATTGAAAAATCTACATTTAATCATAACGGTAGCCAATTCGGTTCCTGGC  >  1/1416408‑1416869                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                   gTGAAGAAATCGTTATTACAAAAAGTCGGTAAACAGGCGGAAGAAAGTGCCGATAATATTGCTTTACGTTGGCTTAAGAAAAATGAAAGAACATTGATATAACATCCTATTTTTTTCGATTCTATTTATACAGATTGATTAAAAATAGAAAACTCGGATTTACTTTAATCCGTTTTGTAATTACCATACGGAGCTGAAATTGAAGTTGCACTATTGCTTTTTGAGCCTTCTTTTTATTATATc                                                                                                                                                                                                                              <  1:611922/243‑1 (MQ=255) gTGAAGAAATCGTTATTACAAAAAGTCGGTAAACAGGCGGAAGAAAGTGCCGATAATATTGCTTTACGTTGGCTTAAGAAAAATGAAAGAACATTGATATAACATCCTATTTTTTTCGATTCTATTTATACAGATTGATTAAAAATAGAAAACTCGGATTTACTTTAATCCGTTTTGTAATTACCATACGGAGCTGAAATTGAAGTTGCACTATTGCTTTTTGAGCCTTCTTTTTATTATATCTATGGtt                                                                                                                                                                                                                       >  1:64488/1‑250 (MQ=255)  tGAAGAAATCGTTATTACAAAAAGTCGGTAAACAGGCGGAAGAAAGTGCCGATAATATTGCTTTACGTTGGCTTAAGAAAAATGAAAGAACATTGATATAACATCCTATTTTTTTCGATTCTATTTATACAGATTGATTAAAAATAGAAAACTCGGATTTACTTTAATCCGTTTTGTAATTACCATACGGAGCTGAAATTGAAGTTGCACTATTGCTTTTTGAGCCTTCTTTTTATTATATCTATGGTTc                                                                                                                                                                                                                      <  1:288813/250‑1 (MQ=255)           cGTTATTACAAAAAGTCGGTAAACAGGCGGAAGAAAGTGCCGATAATATTGCTTTACGTTGGCTTAAGAAAAATGAAAGAACATTGATATAACATCCTATTTTTTTCGATTCTATTTATACAGATTGATTAAAAATAGAAAACTCGGATTTACTTTAATCCGTTTTGTAATTACCATACGGAGCTGAAATTGAAGTTGCACTATTGCTTTTTGAGCCTTCTTTTTATTATATCTATGGTTCCTTTGGAt                                                                                                                                                                                                              >  1:561012/1‑249 (MQ=255)                   aaaaaaGTCGGTAAACAGGCGGAAAAAAGTGCCGATAATATTGCTTTACGTTGGCTTAAAAAAAATGAAAGAACATTGATATAACAACCTATTTTTTTCGATTCTATTTATACAGATTGATTAAAAATAGAAAACTCGGATTTACTTTAATCCGTTTTGTAATTACCATACGGAGCTGAAATTGAAGTTGCACTATTGCTTTTTGAGCCTTCTTTTTATTATATCTATGGTTCCTTTGGATTGTTCTACTc                                                                                                                                                                                                    <  1:737566/250‑1 (MQ=255)                                                                  aCGTTGGCTTAAGAAAAATGAAAGAACATTGATATAACATCCTATTTTTTTCGATTCTATTTATACAGATTGATTAAAAATAGAAAACTCGGATTTACTTTAATCCGTTTTGTAATTACCATACGGAGCTGAAATTGAAGTTGCACTATTGCTTTTTGAGCCTTCTTTTTATTATATCTATGGTTCCTTTGGATTGTTCTACTCACCTAAAAATAAGGTAAAAc                                                                                                                                                                                <  1:663293/224‑1 (MQ=255)                                                                                                gATATAACATCCTATTTTTTTCGATTCTATTTATACAGATTGATTAAAAATAGAAAACTCGGATTTACTTTAATCCGTTTTGTAATTACCATACGGAGCTGAAATTGAAGTTGCACTATTGCTTTTTGAGCCTTCTTTTTATTATATCTATGGTTCCTTTGGATTGTTCTACTCACCTAAAAATAAGGTAAAACAGATGAAATTATCACATTTCTTATTATCTGCAATCGCAGCGGCAACTCTTGTTGCtt                                                                                                                       >  1:872490/1‑251 (MQ=255)                                                                                                        aTCCTATTTTTTTCGATTCTATTTATACAGATTGATTAAAAATAGAAAACTCGGATTTACTTTAATCCGTTTTGTAATTACCATACGGAGCTGAAATTGAAGTTGCACTATTGCTTTTTGAGCCTTCTTTTTATTATATCTATGGTTc                                                                                                                                                                                                                      >  1:824891/1‑148 (MQ=255)                                                                                                                          cTATTTATACAGATTGATTAAAAATAGAAAACTCGGATTTACTTTAATCCGTTTTGTAATTACCATACGGAGCTGAAATTGAAGTTGCACTATTGCTTTTTGAGCCTTCTTTTTATTATATCTATGGTTCCTTTGGATTGTTc                                                                                                                                                                                                         <  1:880380/143‑1 (MQ=255)                                                                                                                          cTATTTATACAGATTGATTAAAAATAGAAAACTCGGATTTACTTTAATCCGTTTTGTAATTACCATACGGAGCTGAAATTGAAGTTGCACTATTGCTTTTTGAGCCTTCTTTTTATTATATCTATGGTTCCTTTGGATTGTTCTACTCACCTAAAAATAAGGTAAAAc                                                                                                                                                                                >  1:77781/1‑168 (MQ=255)                                                                                                                          cTATTTATACAGATTGATTAAAAATAGAAAACTCGGATTTACTTTAATCCGTTTTGTAATTACCATACGGAGCTGAAATTGAAGTTGCACTATTGCTTTTTGAGCCTTCTTTTTATTATATCTATGGTTCCTTTGGATTGTTCTACTCACCTAAAAATAAGGTAAAACAGATGAAATTATCACATTTCTTATTATCTGCAATCGCAGCGGCAACTCTTGTTGCTTGTGGTAACTTa                                                                                                            <  1:866680/236‑1 (MQ=255)                                                                                                                          cTATTTATACAGATTGATTAAAAATAGAAAACTCGGATTTACTTTAATCCGTTTTGTAATTACCATACGGAGCTGAAATTGAAGTTGCACTATTGCTTTTTGAGCCTTCTTTTTATTATATCTATGGTTCCTTTGGATTGTTCTACTCACCTAAAAATAAGGTAAAACAGATGAAATTATCACATTTCTTATTATCTGCAATCGCAGCGGCAACTCTTGTTGCTTGTGGTAACTTAAGTAAAGTATCTaaa                                                                                             >  1:780165/1‑251 (MQ=255)                                                                                                                          cTATTTATACAGATTGATTAAAAATAGAAAACTCGGATTTACTTTAATCCGTTTTGTAATTACCATACGGAGCTGAAATTGAAGTTGCACTATTGCTTTTTGAGCCTTCTTTTTATTATATCTATGGTTCCTTTGGATTGTTCTACTCACCTAAAAATAAGGTAAAACAGATGAAATTATCACATTTCTTATTATCTGCAATCGCAGCGGCAACTCTTGTTGCTTGTGGTAACTTAAGTAAAGTATCTaaa                                                                                             >  1:691544/1‑251 (MQ=255)                                                                                                                           tATTTATACAGATTGATTAAAAATAGAAAACTCGGATTTACTTTAATCCGTTTTGTAATTACCATACGGAGCTGAAATTGAAGTTGCACTATTGCTTTTTGAGCCTTCTTTTTATTATATCTATGGTTCCTTTGGATTGTTCTACTCACCTAAAAATAAGGTAAAACAGATGAAATTATCACATTTCTTATTATCTGCAATCGCAGCGGCAACTCTTGTTGCTTGTGGTAACTTAAGTAAAGTATCTAaag                                                                                            <  1:114305/251‑1 (MQ=255)                                                                                                                            aTTTATACAGATTGATTAAAAATAGAAAACTCGGATTTACTTTAATCCGTTTTGTAATTACCATACGGAGCTGAAATTGAAGTTGCACTATTGCTTTTTGAGCCTTCTTTTTATTATATCTATGGTTCCTTTGGATTGTTCTACTCACCTAAAAATAAGGTAAAACAGATGAAATTATCACATTTCttat                                                                                                                                                        <  1:99612/190‑1 (MQ=255)                                                                                                                                         gattAAAAATAGAAAACTCGGATTTACTTTAATCCGTTTTGTAATTACCATACGGAGCTGAAATTGAAGTTGCACTATTGCTTTTTGAGCCTTCTTTTTATTATATCTATGGTTCCTTTGGATTGTTCTACTc                                                                                                                                                                                                    >  1:158878/1‑133 (MQ=255)                                                                                                                                         gattAAAAATAGAAAACTCGGATTTACTTTAATCCGTTTTGTAATTACCATACGGAGCTGAAATTGAAGTTGCACTATTGCTTTTTGAGCCTTCTTTTTATTATATCTATGGTTCCTTTGGATTGTTCTACTCACCTAAAAAt                                                                                                                                                                                          <  1:655606/143‑1 (MQ=255)                                                                                                                                                       aaCTCGGATTTACTTTAATCCGTTTTGTAATTACCATACGGAGCTGAAATTGAAGTTGCACTATTGCTTTTTGAGCCTTCTTTTTATTATATCTATGGTTCCTTTGGATTGTTCTACTCACCTAAAAATAAGGTAAAACAGATGaaa                                                                                                                                                                        <  1:256993/147‑1 (MQ=255)                                                                                                                                                             gATTTACTTTAATCCGTTTTGTAATTACCATACGGAGCTGAAATTGAAGTTGCACTATTGCTTTTTGAGCCTTCTTTTta‑ttat                                                                                                                                                                                                                                <  1:822313/84‑4 (MQ=255)                                                                                                                                                              aTTTACTTTAATCCGTTTTGTAATTACCATACGGAGCTGAAATTGAAGTTGCACTATTGCTTTTTGAGCCTTCTTTTTATTATATCTATGGTTCCTTTGGATTGTTCTACTCACCTAAAAAt                                                                                                                                                                                          <  1:229531/122‑1 (MQ=255)                                                                                                                                                                  aCTTTAATCCGTTTTGTAATTACCATACGGAGCTGAAATTGAAGTTGCACTATTGCTTTTTGAGCCTTCTTTTTATTATATCTATGGTTCCTTTGGATTGTTCTACTCACCTAAAAAt                                                                                                                                                                                          >  1:572843/1‑118 (MQ=255)                                                                                                                                                                  aCTTTAATCCGTTTTGTAATTACCATACGGAGCTGAAATTGAAGTTGCACTATTGCTTTTTGAGCCTTCTTTTTATTATATCTATGGTTCCTTTGGATTGTTCTACTCACCTAAAAATAAGGTAAAACAGATGAAATTATCACATTTCTTATTATCTGCAATCGCAGCGGCAACTCTTGTTGCTTGTGGTAACTTAAGTAAAGTATCTAAAGAAGGTACTCCTGCCGAAGGTTCTGAATTAGTATGGCCt                                                      >  1:310245/1‑250 (MQ=255)                                                                                                                                                                   cTTTAATCCGTTTTGTAATTACCATACGGAGCTGAAATTGAAGTTGCACTATTGCTTTTTGAGCCTTCTTTTTATTATATCTATGGTTCCTTTGGATTGTTCTACTCACCTAAAAATAAGGTAAAACAGATGAAATTATCACATTTCTTATTATCTGCAATCGCAGCGGCAACTCTTGTTGCTTGTGGTAACt                                                                                                              <  1:94957/193‑1 (MQ=255)                                                                                                                                                                   cTTTAATCCGTTTTGTAATTACCATACGGAGCTGAAATTGAAGTTGCACTATTGCTTTTTGAGCCTTCTTTTTATTATATCTATGGTTCCTTTGGATTGTTCTACTCACCTAAAAATAAGGTAAAACAGATGAAATTATCACATTTCTTATTATCTGCAATCGCAGCGGCAACTCTTGTTGCTTGTGGTAACTTAAGTAAAGTATCTAAAGAAGGTACTCCTGCCGAAGGTTCTGAATTAGTATGGCCTCa                                                    >  1:677299/1‑251 (MQ=255)                                                                                                                                                                      tAATCCGTTTTGTAATTACCATACGGAGCTGAAATTGAAGTTGCACTATTGCTTTTTGAGCCTTCTTTTTATTATATCTATGGTTCCTTTGGATTGTTCTACTCACCTAAAAATAAGGTAAAACAGATGAAATTATCACATTTCTTATTATCTGCAATCGCAGCGGCAACTCTTGTTGCTTGTGGTAACTTAAGTAAAGTATCTAAAGAAGGTACTCCTGCCGAAGGTTCTGAATTAGTATGGCCTCAAAt                                                 <  1:718607/251‑1 (MQ=255)                                                                                                                                                                      tAATCCGTTTTGTAATTACCATACGGAGCTGAAATTGAAGTTGCACTATTGCTTTTTGAGCCTTCTTTTTATTATATCTATGGTTCCTTTGGATTGTTCTACTCACCTAAAAATAAGGTAAAACAGATGAAATTATCACATTTCTTATTATCTGCAATCGCAGCGGCAACTCTTGTTGCTTGTGGTAACTTAAGTAAAGTATCTAAAGAAGGTACTCCTGCCGAAGGTTCTGAATTAGTATGGCCTCAAAt                                                 <  1:43991/251‑1 (MQ=255)                                                                                                                                                                            gTTTTGTAATTACCATACGGAGCTGAAATTGAAGTTGCACTATTGCTTTTTGAGCCTTCTTTTTATTATATCTATGGTTCCTTTGGATTGTTCTACTCACCTAAAAATAAGGTAAAACAGATGAAATTATCACATTTCTTATTATCTGCAATCGCAGCGGCAACTCTTGTTGCTTGTGGTAACTTAAGTAAAGTATCTAAAGAAGGTACTCCTGCCGAAGGTTCTGAATTAGTATGGCCTCAAATTGaaaa                                           >  1:393364/1‑251 (MQ=255)                                                                                                                                                                            gTTTTGTAATTACCATACGGAGCTGAAATTGAAGTTGCACTATTGCTTTTTGAGCCTTCTTTTTATTATATCTATGGTTCCTTTGGATTGTTCTACTCACCTAAAAATAAGGTAAAACAGATGAAATTATCACATTTCTTATTATCTGCAATCGCAGCGGCAACTCTTGTTGCTTGTGGTAACTTAAGTAAAGTATCTAAAGAAGGTACTCCTGCCGAAGGTTCTGAATTAGTATGGCCTCAAATTGaaaa                                           >  1:436531/1‑251 (MQ=255)                                                                                                                                                                                tGTAATTACCATACGGAGCTGAAATTGAAGTTGCACTATTGCTTTTTGAGCCTTCTTTTTATTATATCTATGGTTCCTTTGGATTGTTc                                                                                                                                                                                                         >  1:187786/1‑89 (MQ=255)                                                                                                                                                                                 gTAATTACCATACGGAGCTGAAATTGAAGTTGCACTATTGCTTTTTGAGCCTTCTTTTTATTATATCTATGGTTCCTTTGGATTGTTCTACTCACCTAAAAAt                                                                                                                                                                                          <  1:472015/103‑1 (MQ=255)                                                                                                                                                                                       aCCATACGGAGCTGAAATTGAAGTTGCACTATTGCTTTTTGAGCCTTCTTTTTATTATATCTATg                                                                                                                                                                                                                          >  1:216628/1‑65 (MQ=255)                                                                                                                                                                                          aTACGGAGCTGAAATTGAAGTTGCACTATTGCTTTTTGAGCCTTCTTTTTATTATATCTATGGTTCCTTTGGATTGTTCTACTCACCTAAAAATAAGGTAAAACAGATGAAATTATCACATTTCTTATTATCTGCAATCGCAGCGGCAACTCTTGTTGCTTGTGGTAACTTaagtaa                                                                                                       >  1:642926/1‑177 (MQ=255)                                                                                                                                                                                               gAGCTGAAATTGAAGTTGCACTATTGCTTTTTGAGCCTTCTTTTTATTATATCTATGGTTCCTTTGGATTGTTCTACTCACCTAAAAATAAGGTAAAACAGATGAAATTATCACATTTc                                                                                                                                                            >  1:188184/1‑119 (MQ=255)                                                                                                                                                                                                 gCTGAAATTGAAGTTGCACTATTGCTTTTTGAGCCTTCTTTTTATTATATCTATGGTTCCTTTGGATTGTTCTACTCACCTAAAAATAAGGTAAAACAGATGAAATTATCACATTTCTTATTATCTGCAATCGCAGCGGCAACTCTTGTTGCTTGTGGTAACTTAAGTAAAGTATCTAAAGAAGGTACTCCTGCCGAAGGTTCTGAATTAGTATGGCCTCAAATTGAAAAATCTACATTTAATCATAACgg                      >  1:692296/1‑251 (MQ=255)                                                                                                                                                                                                      aaTTGAAGTTGCACTATTGCTTTTTGAGCCTTCTTTTTATTATATCTATGGTTCCTTTGGATTGTTCTACTCACCTAAAAATAAGGTAAAACAGATGAAATTATCACATTTCTTATTATCTGCAATCGCAGCGGCAACTCTTGTTGCTTGTGGTAACTTAAGTAAAGTATCTAAAGAAGGTACTCCTGCCGAAGGTTCTGAATTAGTATGGCCTCAAATTGAAAAATCTACATTTAATCATAACGGTAGc                  >  1:394576/1‑250 (MQ=255)                                                                                                                                                                                                                     aTTGCTTTTTGAGCCTTCTTTTTATTATATCTATGGTTCCTTTGGATTGTTCTACTCACCTAAAAATAAGGTAAAACAGATGAAATTATCACATTTCTTATTATCTGCAATCGCAGCGGCAACTCTTGTTGCTTGTGGTAACTTAAGTAAAGTATCTAAAGAAGGTACTCCTGCCGAAGGTTCTGAATTAGTATGGCCTCAAATTGAAAAATCTACATTTAAt                              >  1:201781/1‑223 (MQ=255)                                                                                                                                                                                                                     aTTGCTTTTTGAGCCTTCTTTTTATTATATCTATGGTTCCTTTGGATTGTTCTACTCACCTAAAAATAAGGTAAAACAGATGAAATTATCACATTTCTTATTATCTGCAATCGCAGCGGCAACTCTTGTTGCTTGTGGTAACTTAAGTAAAGTATCTAAAGAAGGTACTCCTGCCGAAGGTTCTGAATTAGTATGGCCTCAAATTGAAAAATCTACATTTAATCATAACGGTAGCCAATTCGGTTCCTGGc  <  1:183859/251‑1 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                   GTGAAGAAATCGTTATTACAAAAAGTCGGTAAACAGGCGGAAGAAAGTGCCGATAATATTGCTTTACGTTGGCTTAAGAAAAATGAAAGAACATTGATATAACATCCTATTTTTTTCGATTCTATTTATACAGATTGATTAAAAATAGAAAACTCGGATTTACTTTAATCCGTTTTGTAATTACCATACGGAGCTGAAATTGAAGTTGCACTATTGCTTTTTGAGCCTTCTTTTTA‑TATATCTATGGTTCCTTTGGATTGTTCTACTCACCTAAAAATAAGGTAAAACAGATGAAATTATCACATTTCTTATTATCTGCAATCGCAGCGGCAACTCTTGTTGCTTGTGGTAACTTAAGTAAAGTATCTAAAGAAGGTACTCCTGCCGAAGGTTCTGAATTAGTATGGCCTCAAATTGAAAAATCTACATTTAATCATAACGGTAGCCAATTCGGTTCCTGGC  >  1/1416408‑1416869

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑