Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	2,091,802	2 bp→GA	intergenic (‑35919/–)	unknown ← / –	tRNA‑Met‑CAT/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	2,091,802	0	A	G	100.0%	196.2 / NA	68	intergenic (‑35919/–)	unknown/–	tRNA‑Met‑CAT/–
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (35/33);  total (35/33)
*	1	2,091,803	0	C	A	100.0%	181.5 / NA	68	intergenic (‑35920/–)	unknown/–	tRNA‑Met‑CAT/–
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (35/33);  total (35/33)

TCGGAATCATAAACATATTCGCCACACTGTGCTCAAACCCTGAGGCCACAAACAAAGCAATCGGTAAAATCATAATGAAGGCTTTATCTGTTAACGTTTTTCCCGCGTATGTCATCCAAATGGCAATACACACCATGATATTACATAAAATACCTAAGCAGAACGCTTCAATCCATGTATGGTGAATCTTATGTTGCGCCGTACTTAAAATGGTGAGCCCCCACTGCCCGTGAGCAACCATTGTCTGACCGGAAAACCAAATGACTAAAACAATAAAAATTGCGCCGACGAAATTACCACAATAAACTACAACCCAATTTCGCAGCATTTGAAACGTACTGATACGACCACCGGCACGGGCAACTGTCGTCATTGTAGAAGAAGTAAATAACTCGGAGCCGCATACCACTACCATAATGACCCCTAACGAGAACACGATCCCGCCGACTAATTTCGTCAACCCCCAGGAAC  >  1/2091566‑2092036                                                                                                                                                                                                                                             ||                                                                                                                                                                                                                                          tCGGAATCATAAACATATTCGCCACACTGTGCTCAAACCCTGAGGCCACAAACAAAGCAATCGGTAAAATCATAATGAAGGCTTTATCTGTTAACGTTTTTCCCGCGTATGTCATCCAAATGGCAATACACACCATGATATTACATAAAATACCTAAGCAGAACGCTTCAATCCATGTATGGTGAATCTTATGTTGCGCCGTACTTAAAATGGTGAGCCCCCACTGCCCGTGAGCAGACATTGTCTGACCg                                                                                                                                                                                                                              <  1:500645/251‑1 (MQ=255)        cATAAACATATTCGCCACACTGTGCTCAAACCCTGAGGCCACAAACAAAGCAATCGGTAAAATCATAATGAAGGCTTTATCTGTTAACGTTTTTCCCGCGTATGTCATCCAAATGGCAATACACACCATGATATTACATAAAATACCTAAGCAGAACGCTTCAATCCATGTATGGTGAATCTTATGTTGCGCCGTACTTAAAATGGTGAGCCCCCACTGCCCGTGAGCAGACATTGTc                                                                                                                                                                                                                                    >  1:53102/1‑238 (MQ=255)                   tCGCCACACTGTGCTCAAACCCTGAGGCCACAAACAAAGCAATCGGTAAAATCATAATGAAGGCTTTATCTGTTAACGTTTTTCCCGCGTATGTCATCCAAATGGCAATACACACCATGATATTACATAAAATACCTAAGCAGAACGCTTCAATCCATGTATGGTGAATCTTATGTTGCGCCGTACTTAAAATGGTGAGCCCCCACTGCCCGTGAGCAGACATTGTCTGACCGGAAAACCAAATGACTaaa                                                                                                                                                                                                            >  1:705228/1‑251 (MQ=255)                             gtgCTCAAACCCTGAGGCCACAAACAAAGCAATCGGTAAAATCATAATGAAGGCTTTATCTGTTAACGTTTTTCCCGCGTATGTCATCCAAATGGCAATACACACCATGATATTACATAAAATACCTAAGCAGAACGCTTCAATCCATGTATGGTGAATCTTATGTTGCGCCGTACTTAAAATGGTGAGCCCCCACTGCCCGTGAGCAGACATTGTCTGACCGGAAAAc                                                                                                                                                                                                                        <  1:260535/229‑1 (MQ=255)                             gtgCTCAAACCCTGAGGCCACAAACAAAGCAATCGGTAAAATCATAATGAAGGCTTTATCTGTTAACGTTTTTCCCGCGTATGTCATCCAAATGGCAATACACACCATGATATTACATAAAATACCTAAGCAGAACGCTTCAATCCATGTATGGTGAATCTTATGTTGCGCCGTACTTAAAATGGTGAGCCCCCACTGCCCGTGAGCAGACATTGTCTGACCGGAAAACCAAATGACTAAAACAATaaaaa                                                                                                                                                                                                  >  1:49368/1‑251 (MQ=255)                             gtgCTCAAACCCTGAGGCCACAAACAAAGCAATCGGTAAAATCATAATGAAGGCTTTATCTGTTAACGTTTTTCCCGCGTATGTCATCCAAATGGCAATACACACCATGATATTACATAAAATACCTAAGCAGAACGCTTCAATCCATGTATGGTGAATCTTATGTTGCGCCGTACTTAAAATGGTGAGCCCCCACTGCCCGTGAGCAGACATTGTCTGACCGGAAAACCAAATGACTAAAACAATaaaaa                                                                                                                                                                                                  >  1:679452/1‑251 (MQ=255)                                                            aTCGGTAAAATCATAATGAAGGCTTTATCTGTTAACGTTTTTCCCGCGTATGTCATCCAAATGGCAATACACACCATGATATTACATAAAATACCTAAGCAGAACGCTTCAATCCATGTATGGTGAATCTTATGTTGCGCCGTACTTAAAATGGTGAGCCCCCACTGCCCGTGAGCAGACATTGTCTGACCGGAAAACCAAATGACTAAAACAATAAAAATTGCGCCGACGAAATTACCACAATAAACTAc                                                                                                                                                                   <  1:563519/251‑1 (MQ=255)                                                                    aaTCATAATGAAGGCTTTATCTGTTAACGTTTTTCCCGCGTATGTCATCCAAATGGCAATACACACCATGATATTACATAAAATACCTAAGCAGAACGCTTCAATCCATGTATGGTGAATCTTATGTTGCGCCGTACTTAAAATGGTGAGCCCCCACTGCCCGTGAGCAGACATTGTCTGACCGGAAAACCAAATGACTAAAACAATAAAAATTGCGCCGACGAAATTACCACAATAAACTACAACCCAAt                                                                                                                                                           <  1:55688/251‑1 (MQ=255)                                                                                 gCTTTATCTGTTAACGTTTTTCCCGCGTATGTCATCCAAATGGCAATACACACCATGATATTACATAAAATACCTAAGCAGAACGCTTCAATCCATGTATGGTGAATCTTATGTTGCGCCGTACTTAAAATGGTGAGCCCCCACTGCCCGTGAGCAGACATTGTCTGACCGGAAAACCAAATGACTAAAACAATAAAAATTGCGCCGACGAAATTACCACAATAAACTACAACCCAATTTCGCAGCATTTg                                                                                                                                              >  1:3460/1‑251 (MQ=255)                                                                                      aTCTGTTAACGTTTTTCCCGCGTATGTCATCCAAATGGCAATACACACCATGATATTACATAAAATACCTAAGCAGAACGCTTCAATCCATGTATGGTGAATCTTATGTTGCGCCGTACTTAAAATGGTGAGCCCCCACTGCCCGTGAGCAGACATTGTCTGACCGGAAAACCAAATGACTAAAACAATAAAAATTGCGCCGACGAAATTACCACAATAAACTACAACCCAATTTCGCAGCATTTGAAACg                                                                                                                                         >  1:406549/1‑251 (MQ=255)                                                                                                   tttCCCGCGTATGTCATCCAAATGGCAATACACACCATGATATTACATAAAATACCTAAGCAGAACGCTTCAATCCATGTATGGTGAATCTTATGTTGCGCCGTACTTAAAATGGTGAGCCCCCACTGCCCGTGAGCAGACATTGTCTGACCGGAAAACCAAATGACTAAAACAATAAAAATTGCGCCGACGAAATTACCACAATAAACTACAACCCAATTTCGCAGCATTTGAAACGTACTGATACGacc                                                                                                                            <  1:464550/251‑1 (MQ=255)                                                                                                        cgcgTATGTCATCCAAATGGCAATACACACCATGATATTACATAAAATACCTAAGCAGAACGCTTCAATCCATGTATGGTGAATCTTATGTTGCGCCGTACTTAAAATGGTGAGCCCCCACTGCCCGTGAGCAGACATTGTCTGACCGGAAAACCAAATGACTAAAACAATAAAAATTGCGCCGACGAAATTACCACAATAAACTACAACCCAATTTCGCAGCATTTGAAACGTACTGATACGACCACCgg                                                                                                                       >  1:893994/1‑251 (MQ=255)                                                                                                             aTGTCATCCAAATGGCAATACACACCATGATATTACATAAAATACCTAAGCAGAACGCTTCAATCCATGTATGGTGAATCTTATGTTGCGCCGTACTTAAAATGGTGAGCCCCCACTGCCCGTGAGCAGACATTGTCTGACCGGAAAACCAAATGACTAAAACAATAAAAATTGCGCCGACGAAATTACCACAATAAACTACAACCCAATTTCGCAGCATTTGAAACGTACTGATa                                                                                                                                 >  1:194406/1‑236 (MQ=255)                                                                                                                    ccAAATGGCAATACACACCATGATATTACATAAAATACCTAAGCAGAACGCTTCAATCCATGTATGGTGAATCTTATGTTGCGCCGTACTTAAAATGGTGAGCCCCCACTGCCCGTGAGCAGACATTGTCTGACCGGAAAACCAAATGACTAAAACAATAAAAATTGCGCCGACGAAATTACCACAATAAACTACAACCCAATTTCGCAGCATTTGAAACGTACTGATACGACCACCGGCACGGGCaa                                                                                                              >  1:187740/1‑248 (MQ=255)                                                                                                                           gCAATACACACCATGATATTACATAAAATACCAAAGCAGAACGCTTCAATCCATGTATGGTGAATCTTATGTTGCGCCGTACTTAAAATGGTGAGCCCCCACTGCCCGTGAGCAGACATTGTCTGACCGGAAAACCAAATGACTAAAACAATAAAAATTGCGCCGACGAAATTACCACAATAAACTACAACCCAATTTCGCAGCATTTGAAACGTACTGATACGACCACCGGCACGGGCAACTGTCGTCAt                                                                                                    >  1:828591/1‑251 (MQ=255)                                                                                                                             aaTACACACCATGATATTACATAAAATACCTAAGCAGAACGCTTCAATCCATGTATGGTGAATCTTATGTTGCGCCGTACTTAAAATGGTGAGCCCCCACTGCCCGTGAGCAGACATTGTCTGACCGGAAAACCAAATGACTAAAACAATAAAAATTGCGCCGACGAAATTACCACAATAAACTACAACCCAATTTCGCAGCATTTGAAACGTACTGATACGACCACCGGCACGGGCAACTGTCGTCATTg                                                                                                  >  1:384296/1‑251 (MQ=255)                                                                                                                              aTACACACCATGATATTACATAAAATACCTAAGCAGAACGCTTCAATCCATGTATGGTGAATCTTATGTTGCGCCGTACTTAAAATGGTGAGCCCCCACTGCCCGTGAGCAGACATTGTCTGACCGGAAAACCAAATGACTAAAACAATAAAAATTGCGCCGACGAAATTACCACAATAAACTACAACCCAAttt                                                                                                                                                         >  1:345941/1‑195 (MQ=255)                                                                                                                                   cacCATGATATTACATAAAATACCTAAGCAGAACGCTTCAATCCATGTATGGTGAATCTTATGTTGCGCCGTACTTAAAATGGTGAGCCCCCACTGCCCGTGAGCAGACATTGTCTGACCGGAAAACCAAATGACTAAAACAATAAAAATTGCGCCGACGAAATTACCACAATAAACTACAACCCAATTTCGCAGCATTTGAAACGTACTGATACGACCACCGGCACGGGCAACTGTCGTCATTg                                                                                                  <  1:481021/245‑1 (MQ=255)                                                                                                                                     ccATGATATTACATAAAATACCTAAGCAGAACGCTTCAATCCATGTATGGTGAATCTTATGTTGCGCCGTACTTAAAATGGTGAGCCCCCACTGCCCGTGAGCAGACATTGTCTGACCGGAAAACCAAATGACTAAAACAATAAAAATTGCGCCGACGAAATTACCACAATAAACTACAACCCAATTTCGCAGCATTTGAAACGTACTGATa                                                                                                                                 >  1:643788/1‑212 (MQ=255)                                                                                                                                     ccATGATATTACATAAAATACCTAAGCAGAACGCTTCAATCCATGTATGGTGAATCTTATGTTGCGCCGTACTTAAAATGGTGAGCCCCCACTGCCCGTGAGCAGACATTGTCTGACCGGAAAACCAAATGACTAAAACAATAAAAATTGCGCCGACGAAATTACCACAATAAACTACAACCCAATTTCGCAGCATTTGAAACGTACTGATACGACCACCGGCACGGGCAACTgt                                                                                                          >  1:299808/1‑235 (MQ=255)                                                                                                                                        tGATATTACATAAAATACCTAAGCAGAACGCTTCAATCCATGTATGGTGAATCTTATGTTGCGCCGTACTTAAAATGGTGAGCCCCCACTGCCCGTGAGCAGACATTGTCTGACCGGAAAACCAAATGACTAAAAc                                                                                                                                                                                                          >  1:514292/1‑136 (MQ=255)                                                                                                                                        tGATATTACATAAAATACCTAAGCAGAACGCTTCAATCCATGTATGGTGAATCTTATGTTGCGCCGTACTTAAAATGGTGAGCCCCCACTGCCCGTGAGCAGACATTGTCTGACCGGAAAACCAAATGACTAAAACAATAAAAATTGCGCCGACGAAATTACCACAATAAACTACAACCCAATTTCGCAGCATTTGAAACGTACTGATACGacc                                                                                                                            <  1:545517/214‑1 (MQ=255)                                                                                                                                          atatTACATAAAATACCTAAGCAGAACGCTTCAATCCATGTATGGTGAATCTTATGTTGCGCCGTACTTAAAATGGTGAGCCCCCACTGCCCGTGAGCAGACATTGTCTGACCGGAAAACCAAATGACTAAAACAATAAAAATTGCGCCGACGAAATTACCACAATAAACTACAACCAAATTTCGCAGCATTTGAAACGTACTGATACGACCACCGGCACGGGCAACTGTCGTCATTGTag                                                                                               >  1:298099/1‑241 (MQ=255)                                                                                                                                              tACATAAAATACCTAAGCAGAACGCTTCAATCCATGTATGGTGAATCTTATGTTGCGCCGTACTTAAAATGGTGAGCCCCCACTGCCCGTGAGCAGACATTGTCTGACCGGAAAACCAAATGACTAAAACAATAAAAATTGCGCCGACGAAATTACCACAATAAACTACAACCCAATTTCGCAGCATTTGAAACGTACTGATACGACCACCGGCACGGGCAACTGTCGTCATTGTAGAAGAAGTAAATAAc                                                                                 <  1:188937/251‑1 (MQ=255)                                                                                                                                               aCATAAAATACCTAAGCAGAACGCTTCAATCCATGTATGGTGAATCTTATGTTGCGCCGTACTTAAAATGGTGAGCCCCCACTGCCCGTGAGCAGACATTGTCTGACCGGAAAACCAAATGACTAAAACAATAAAAATTGCGCCGACGAAATTACCACAATAAACTACAACCCAATTTCGCAGCATTTGAAACGTACTGATACGACCACCGGCACGGGCAACTGTCGTCATTGTAGAAGAAGTAAAt                                                                                    >  1:1767/1‑247 (MQ=255)                                                                                                                                               aCATAAAATACCTAAGCAGAACGCTTCAATCCATGTATGGTGAATCTTATGTTGCGCCGTACTTAAAATGGTGAGCCCCCACTGCCCGTGAGCAGACATTGTCTGACCGGAAAACCAAATGACTAAAACAATAAAAATTGCGCCGACGAAATTACCACAATAAACTACAACCCAATTTCGCAGCATTTGAAACGTACTGATACGACCACCGGCACGGGCAACTGTCGTCATTGTAGAAGAAGTAAATaa                                                                                  >  1:780339/1‑249 (MQ=255)                                                                                                                                                cATAAAATACCTAAGCAGAACGCTTCAATCCATGTATGGTGAATCTTATGTTGCGCCGTACTTAAAATGGTGAGCCCCCACTGCCCGTGAGCAGACATTGTCTGACCGGAAAACCAAATGACTAAAACAATAAAAATTGCGCCGACGAAATTACCACAATAAACTACAACCCAATTTCGCAGCATTTGAAACGTACTGATACGACCACCGGCACGGGCAACTGTCGTCATTGTAGAAGAAGTAAATAACTc                                                                               <  1:901093/251‑1 (MQ=255)                                                                                                                                                 aTAAAATACCTAAGCAGAACGCTTCAATCCATGTATGGTGAATCTTATGTTGCGCCGTACTTAAAATGGTGAGCCCCCACTGCCCGTGAGCAGACATTGTCTGACCGGAAAACCAAATGACTAAAACAATAAAAATTGCGCCGACGAAATTACCACAATAAACTACAACCCAATTTCGCAGCATTTGAAACGTACTGATACGACCACCGGCACGGGCAACTGTCGTCATTGTAGAAGAAGTAAATAACTCg                                                                              >  1:837020/1‑251 (MQ=255)                                                                                                                                                  tAAAATACCTAAGCAGAACGCTTCAATCCATGTATGGTGAATCTTATGTTGCGCCGTACTTAAAATGGTGAGCCCCCACTGCCCGTGAGCAGACATTGTCTGACCGGAAAACCAAATGACTAAAACAATAAAAATTGCGCCGACGAAATTACCACAATAAACTACAACCCAATTTCGCAGCATTTGAAACGTACTGATACGACCACCGGCACGGGCAACTGTCGTCa                                                                                                     <  1:891360/227‑1 (MQ=255)                                                                                                                                                  tAAAATACCTAAGCAGAACGCTTCAATCCATGTATGGTGAATCTTATGTTGCGCCGTACTTAAAATGGTGAGCCCCCACTGCCCGTGAGCAGACATTGTCTGACCGGAAAACCAAATGACTAAAACAATAAAAATTGCGCCGACGAAATTACCACAATAAACTACAACCCAATTTCGCAGCATTTGAAACGTACTGATACGACCACCGGCACGGGCAACTGTCGTCATTGTAGAAGAAGTAAATAACTCgg                                                                             >  1:207995/1‑251 (MQ=255)                                                                                                                                                       tACCTAAGCAGAACGCTTCAATCCATGTATGGTGAATCTTATGTTGCGCCGTACTTAAAATGGTGAGCCCCCACTGCCCGTGAGCAGACATTGTCTGACCGGAAAACCAAATGACTAAAACAATAAAAATTGCGCCGACGAAATTACCACAATAAACTACAACCCAATTTCGCAGCATTTGAAACGTACTGATa                                                                                                                                 <  1:340907/194‑1 (MQ=255)                                                                                                                                                       tACCTAAGCAGAACGCTTCAATCCATGTATGGTGAATCTTATGTTGCGCCGTACTTAAAATGGTGAGCCCCCACTGCCCGTGAGCAGACATTGTCTGACCGGAAAACCAAATGACTAAAACAATAAAAATTGCGCCGACGAAATTACCACAATAAACTACAACCCAATTTCGCAGCATTTGAAACGTACTGATACGACCACCGGCACGGGCAACTGTCGTCATTGTAGAAGAAGTAAATAACTCGGAGCCg                                                                        >  1:49792/1‑251 (MQ=255)                                                                                                                                                        aCCTAAGCAGAACGCTTCAATCCATGTATGGTGAATCTTATGTTGCGCCGTACTTAAAATGGTGAGCCCCCACTGCCCGTGAGCAGACATTGTCTGACCGGAAAACCAAATGACTAAAACAATAAAAATTGCGCCGACGAAATTACCACAATAAACTACAACCCAATTTCGCAGCATTTGAAACGTACTGATACGACCACCGGCACGGGCAACTGTCGTCATTGTAGAAGAAGTAAATAACTCGGAGCCg                                                                        >  1:165190/1‑250 (MQ=255)                                                                                                                                                        aCCTAAGCAGAACGCTTCAATCCATGTATGGTGAATCTTATGTTGCGCCGTACTTAAAATGGTGAGCCCCCACTGCCCGTGAGCAGACATTGTCTGACCGGAAAACCAAATGACTAAAACAATAAAAATTGCGCCGACGAAATTACCACAATAAACTACAACCCAATTTCGCAGCATTTGAAACGTACTGATACGACCACCGGCACGGGCAACTGTCGTCATTGTAGAAGAAGTAAATAACTCGGAGCCg                                                                        >  1:877744/1‑250 (MQ=255)                                                                                                                                                          cTAAGCAGAACGCTTCAATCCATGTATGGTGAATCTTATGTTGCGCCGTACTTAAAATGGTGAGCCCCCACTGCCCGTGAGCAGACATTGTCTGACCGGAAAACCAAATGACTAAAACAATAAAAATTGCGCCGACGAAATTACCACAATAAACTACAACCCAATTTCGCAGCATTTGAAGCGTACTGATACGACCACCGGCACGGGCAACTGTCGTCATTGTAGAAGAAGTAAATAACTCGGAGCCGCac                                                                     <  1:98504/251‑2 (MQ=255)                                                                                                                                                          cTAAGCAGAACGCTTCAATCCATGTATGGTGAATCTTATGTTGCGCCGTACTTAAAATGGTGAGCCCCCACTGCCCGTGAGCAGACATTGTCTGACCGGAAAACCAAATGACTAAAACAATAAAAATTGCGCCGACGAAATTACCACAATAAACTACAACCCAATTTCGCAGCATTTGAAACGTACTGATACGACCACCGGCACGGGCAACTGTCGTCATTGTAGAAGAAGTAAATAACTCGGAGCCGCac                                                                     <  1:573557/251‑2 (MQ=255)                                                                                                                                                            aaGCAGAACGCTTCAATCCATGTATGGTGAATCTTATGTTGCGCCGCACTTAAAATGGTGAGCCCCCACTGCCCGTGAGCAGACATTGTCTGACCGGAAAACCAAATGACTAAAACAATAAAAATTGCGCCGACGAAATTAcc                                                                                                                                                                               >  1:825737/1‑143 (MQ=255)                                                                                                                                                               cAGAACGCTTCAATCCATGTATGGTGAATCTTATGTTGCGCCGTACTTAAAATGGTGAGCCCCCACTGCCTGTGAGCAGACATTGTCTGACCGGAAAACCAAATGACTAAAACAATAAAAATTGCGCCGACGAAATTACCACAATAAACTACAACCCAATTTCGCAGCATTTGAAACGTACTGATACGACCACCGGCACGGGCAACTGTCGTCATTGTAGAAGAAGTAAATAACTCGGAGCCGCACAcc                                                                  <  1:279257/249‑1 (MQ=255)                                                                                                                                                                 gAACGCTTCAATCCATGTATGGTGAATCTTATGTTGCGCCGTACTTAAAATGGTGAGCCCCCACTGCCCGTGAGCAGACATTGTCTGACCGGAAAACCAAATGACTAAAACAATAAAAATTGCGCCGACGAAATTACCACAATAAACTACAACCCAATTTCGCAGCATTTGAAACGTACTGATACGACCACCGGCACGGGCAACTGTCGTCATTGTAGAAGAAGTAAATAACTCGGAGCCGCACAcc                                                                  <  1:319777/247‑1 (MQ=255)                                                                                                                                                                  aaCGCTTCAATCCATGTATGGTGAATCTTATGTTGCGCCGTACTTAAAATGGTGAGCCCCCACTGCCCGTGAGCAGACATTGTCTGACCGGAAAACCAAATGACTAAAACAATAAAAATTGCGCCGACGAAATTACCACAATAAACTACAACCCAATTTCGCAGCATTTGAAACGTACTGATACGACCACCGGCACGGGCAACTGTCGTCATTGTAGAAGAAGTAAATAACTCGGAGCCGCAcaccac                                                                <  1:536814/248‑1 (MQ=255)                                                                                                                                                                  aaCGCTTCAATCCATGTATGGTGAATCTTATGTTGCGCCGTACTTAAAATGGTGAGCCCCCACTGCCCGTGAGCAGACATTGTCTGACCGGAAAACCAAATGACTAAAACAATAAAAATTGCGCCGACGAAATTACCACAATAAACTACAACCCAATTTCGCAGCATTTGAAACGTACTGATACGACCACCGGCACGGGCAACTGTCGTCATTGTAGAAGAAGTAAATAACTCGGAGCCGCACACCACTAc                                                             <  1:243081/251‑1 (MQ=255)                                                                                                                                                                    cGCTTCAATCCATGTATGGTGAATCTTATGTTGCGCCGTACTTAAAATGGTGAGCCCCCACTGCCCGTGAGCAGACATTGTCTGACCGGAAAACCAAATGACTAAAACAATAAAAATTGCGCCGACGAAATTACCACAATAAACTACAACCCAATTTCGCAGCATTTGAAACGTACTGATACGACCACCGGCACGGGCAACTGTCGTCATTGTAGAAGAAGTAAATAACTCGGAGCCGCACACCACTACCa                                                           <  1:10160/251‑1 (MQ=255)                                                                                                                                                                    cGCTTCAATCCATGTATGGTGAATCTTATGTTGCGCCGTACTTAAAATGGTGAGCCCCCACTGCCCGTGAGCAGACATTGTCTGACCGGAAAACCAAATGACTAAAACAATAAAAATTGCGCCGACGAAATTACCACAATAAACTACAACCCAATTTCGCAGCATTTGAAACGTACTGATACGACCACCGGCACGGGCAACTGTCGTCATTGTAGAAGAAGTAAATAACTCGGAGCCGCACACCACTACCa                                                           <  1:894590/251‑1 (MQ=255)                                                                                                                                                                      cTTCAATCCATGTATGGTGAATCTTATGTTGCGCCGTACTTAAAATGGTGAGCCCCCACTGCCCGTGAGCAGACATTGTCTGACCGGAAAACCAAATGACTAAAACAATAAAAATTGCGCCGACGAAATTACCACAATAAACTACAACCCAATTTCGCAGCATTTGAAACGTACTGATACGACCACCGGCACGGGCAACTGTCGTCATTGTAGAAGAAGTAAATAACTCGGAGCCGCACACCACTACCATa                                                         >  1:654962/1‑251 (MQ=255)                                                                                                                                                                       ttCAATCCATGTATGGTGAATCTTATGTTGCGCCGTACTTAAAATGGTGAGCCCCCACTGCCCGTGAGCAGACATTGTCTGACCGGAAAACCAAATGACTAAAACAATAAAAATTGCGCCGACGAAATTACCACAATAAACTACAACCCAATTTCGCAGCATTTGAAACGTACTGATACGACCACCGGCACGGGCAACTGTCGTCATTGTAGAAGAAGTAAATAACTCGGAGCCGCACACCACTACCATaa                                                        <  1:121309/251‑1 (MQ=255)                                                                                                                                                                         cAATCCATGTATGGTGAATCTTATGTTGCGCCGTACTTAAAATGGTGAGCCCCCACTGCCCGTGAGCAGACATTGTCTGACCGGAAAACCAAATGACTAAAACAATAAAAATTGCGCCGACGAAATTACCACAATAAACTACAACCCAATTTCGCAGCATTTGAAACGTACTGATACGACCACCGGCACGGGCAACTGTCGTCATTGTAGAAGAAGTAAATAACTCGGAGCCGCACACCACTACCa                                                           >  1:747581/1‑246 (MQ=255)                                                                                                                                                                         cAATCCATGTATGGTGAATCTTATGTTGCGCCGTACTTAAAATGGTGAGCCCCCACTGCCCGTGAGCAGACATTGTCTGACCGGAAAACCAAATGACTAAAACAATAAAAATTGCGCCGACGAAATTACCACAATAAACTACAACCCAATTTCGCAGCATTTGAAACGTACTGATACGACCACCGGCACGGGCAACTGTCGTCATTGTAGAAGAAGTAAATAACTCGGAGCCGCACACCACTACCATAATg                                                      <  1:748328/251‑1 (MQ=255)                                                                                                                                                                           aTCCATGTATGGTGAATCTTATGTTGCGCCGTACTTAAAATGGTGAGCCCCCACTGCCCGTGAGCAGACATTGTCTGACCGGAAAACCAAATGACTAAAACAATAAAAATTGCGCCGACGAAATTACCACAATAAACTACAACCCAATTTCGCAGCATTTGAAACGTACTGATACGACCACCGGCACGGGCAACTGTCGTCATTGTAGAAGAAGTAAATAACTCGGAGCCGCACACCACTAcc                                                            >  1:377520/1‑243 (MQ=255)                                                                                                                                                                            tCCATGTATGGTGAATCTTATGTTGCGCCGTACTTAAAATGGTGAGCCCCCACTGCCCGTGAGCAGACATTGTCTGACCGGAAAACCAAATGACTAAAACAATAAAAATTGCGCCGACGAAATTACCACAATAAACTACACCCCAATTTCGCAGCATTTGAAACGTACTGATACGACCGCCGGCACGGGCAACTGTCGTCATTGTagaaga                                                                                           <  1:356312/211‑1 (MQ=255)                                                                                                                                                                              cATGTATGGTGAATCTTATGTTGCGCCGTACTTAAAATGGTGAGCCCCCACTGCCCGTGAGCAGACATTGTCTGACCGGAAAACCAAATGACTAAAACAATAAAAATTGCGCCGACGAAATTACCACAATAAACTACAACCCAATTTCGCAGCATTTGAAACGTACTGATACGACCACCGGCACGGGCAACTGTCGTCATTGTAGAAGAAGTAAATAACTCGGAGCCGCACACCACTACCATAATGAcc                                                   <  1:355705/249‑1 (MQ=255)                                                                                                                                                                               aTGTATGGTGAATCTTATGTTGCGCCGTACTTAAAATGGTGAGCCCCCACTGCCCGTGAGCAGACATTGTCTGACCGGAAAACCAAATGACTAAAACAATAAAAATTGCGCCGACGAAATTACCACAATAAACTACAACCCAATTTCGCAGCATTTGAAACGTACTGATACGACCACCGGCACGGGCAACTGTCGTCATTGTAGAAGAAGTAAATAACTCGGAGCCGCACACCACTACCATaa                                                        <  1:654044/243‑1 (MQ=255)                                                                                                                                                                                tGTATGGTGAATCTTATGTTGCGCCGTACTTAAAATGGTGAGCCCCCACTGCCCGTGAGCAGACATTGTCTGACCGGAAAACCAAATGACTAAAACAATAAAAATTGCGCCGACGAAATTACCACAATaa                                                                                                                                                                        <  1:811043/130‑1 (MQ=255)                                                                                                                                                                                 gTATGGTGAATCTTATGTTGCGCCGTACTTAAAATGGTGAGCCCCCACTGCCCGTGAGCAGACATTGTCTGACCGGAAAACCAAATGACTAAAACAATAAAAATTGCGCCGACGAAATTACCACAATAAACTACAACCCAATTTCGCAGCATTTGAAACGTACTGATACGACCACCGGCACGGGCAACTGTCGTCATTGTAGAAGAAGTAAATAAc                                                                                 >  1:34541/1‑216 (MQ=255)                                                                                                                                                                                   aTGGTGAATCTTATGTTGCGCCGTACTTAAAATGGTGAGCCCCCACTGCCCGTGAGCAGACATTGTCTGACCGGAAAACCAAATGACTAAAACAATAAAAATTGCGCCGACGAAATTACCACAATAAACTACAACCCAATTTCGCAGCATTTGAAACGTACTGATACGACCACCGGCACGGGCAACTGTCGTCATTGTAGAAGAAGTAAATAACTCGGAGCCGCACACCACTACCATAATGACCCCTAACg                                            <  1:126144/251‑1 (MQ=255)                                                                                                                                                                                       tGAATCTTATGTTGCGCCGTACTTAAAATGGTGAGCCCCCACTGCCCGTGAGCAGACATTGTCTGACCGGAAAACCAAATGACTAAAACAATAAAAATTGCGCCGACGAAATTACCACAATAAACTACAACCCAATTTCGCAGCATTTGAAACGTACTGATACGAGCACCGGCACGGGCAACTGTCGTCATTGTAGAAGAAGTAAATAACTCGGAGCCGCACACCACTACCATAATGACCCCTAACgaga                                         <  1:384511/250‑1 (MQ=255)                                                                                                                                                                                             ttATGTTGCGCCGTACTTAAAATGGTGAGCCCCCACTGCCCGTGAGCAGACATTGTCTGACCGGAAAACCAAATGACTAAAACAATAAAAATTGCGCCGACGAAATTACCACAATAAACTACAACCCAATTTCGCAGCATTTGAAACGTACTGATACGACCACCGGCACGGGCAACTGTCGTCATTGTAGAAGAAGTAAATAACTCGGAGCCGCACACCACTACCATAATGACCCCTAACGAGAACACGAt                                  <  1:894833/251‑1 (MQ=255)                                                                                                                                                                                               aTGTTGCGCCGTACTTAAAATGGTGAGCCCCCACTGCCCGTGAGCAGACATTGTCTGACCGGAAAACCAAATGACTAAAACAATAAAAATTGCGCCGACGAAATTACCACAATAAACTACAACCCAATTTCGCAGCATTTGAAACGTACTGATACGACCACCGGCACGGGCAACTGTCGTCAt                                                                                                    >  1:254715/1‑183 (MQ=255)                                                                                                                                                                                                    gcgcCGTACTTAAAATGGTGAGCCCCCACTGCCCGTGAGCAGACATTGTCTGACCGGAAAACCAAATGACTAAAACAATAAAAATTGCGCCGACGAAATTACCACAATAAACTACAACCCAATTTCGCAGCATTTGAAACGTACTGATACGacc                                                                                                                            <  1:890476/154‑1 (MQ=255)                                                                                                                                                                                                         gTACTTAAAATGGTGAGCCCCCACTGCCCGTGAGCAGACATTGTCTGACCGGAAAACCAAATGACTAAAACAATAAAAATTGCGCCGACGAAATTACCACAATAAACTACAACCCAATTTCGCAGCATTTGAAACGTACTGATACGACCACCGGCACGGGCAACTGTCGTCATTGTAGAAGAAGTAAATAACTCGGAGCCGCACACCACTACCa                                                           >  1:591842/1‑214 (MQ=255)                                                                                                                                                                                                            cTTAAAATGGTGAGCCCCCACTGCCCGTGAGCAGACATTGTCTGACCGGAAAACCAAATGACTAAAACAATAAAAATTGCGCCGACGAAATTACCACAATAAACTACAACCCAATTTCGCAGCATTTGAAACGTACTGATACGACCACCGGCACGGGCAACTgtcgtc                                                                                                      >  1:137892/1‑168 (MQ=255)                                                                                                                                                                                                            cTTAAAATGGTGAGCCCCCACTGCCCGTGAGCAGACATTGTCTGACCGGAAAACCAAATGACTAAAACAATAAAAATTGCGCCGACGAAATTACCACAATAAACTACAACCCAATTTCGCAGCATTTGAAACGTACTGATACGACCACCGGCACGGGCAACTGTCGTCATTGTAGAAGAAGTAAATAACTCGGAGCCGCACACCACTACCATAATGACCCCTAACGAGAACACGATCCCGCCGACTAAttt                   >  1:87217/1‑251 (MQ=255)                                                                                                                                                                                                                       gAGCCCCCACTGCCCGTGAGCAGACATTGTCTGACCGGAAAACCAAATGACTAAAACAATAAAAATTGCGCCGACGAAATTACCACAATAAACTACAACCCAATTTCGCAGCATTTGAAACGTACTGATACGACCACCGGCACGGGCAACTGTCGTCATTGTAGAAGAAGTAAATAACTCGGAGCCGCACACCACTACCATAATGACCCCTAACGAGAACACGATCCCGCCGACTAATTTCGTCAAcccc         >  1:187154/1‑250 (MQ=255)                                                                                                                                                                                                                       gAGCCCCCACTGCCCGTGAGCAGACATTGTCTGACCGGAAAACCAAATGACTAAAACAATAAAAATTGCGCCGACGAAATTACCACAATAAACTACAACCCAATTTCGCAGCATTTGAAACGTACTGATACGACCACCGGCACGGGCAACTGTCGTCATTGTAGAAGAAGTAAATAACTCGGAGCCGCACACCACTACCATAATGACCCCTAACGAGAACACGATCCCGCCGACTAATTTCGTCAAcccc         >  1:354670/1‑250 (MQ=255)                                                                                                                                                                                                                          cccccACTGCCCGTGAGCAGACATTGTCTGACCGGAAAACCAAATGACTAAAACAATAAAAATTGCGCCGACGAAATTACCACAATAAACTACAACCCAATTTCGCAGCATTTGAAACGTACTGATACGACCACCGGCACGGGCAACTGTCGTCATTGTAGAAGAAGTAAATAACTCGGAGCCGCACACCACTACCa                                                           >  1:777049/1‑197 (MQ=255)                                                                                                                                                                                                                           ccccACTGCCCGTGAGCAGACATTGTCTGACCGGAAAACCAAATGACTAAAACAATAAAAATTGCGCCGACGAAATTACCACAATAAACTACAACCCAATTTCGCAGCATTTGAAACGTACTGATACGACCACCGGCACGGGCAACTGTCGTCATTGTAGAAGAAGTAAATAACTCGGAGCCGCACACCACTACCATAATGACCCCTAACGAGAACACGATCCCGCCGACTAATTTCGTCAACCCCCAGGa    <  1:231966/251‑1 (MQ=255)                                                                                                                                                                                                                           ccccACTGCCCGTGAGCAGACATTGTCTGACCGGAAAACCAAATGACTAAAACAATAAAAATTGCGCCGACGAAATTACCACAATAAACTACAACCCAATTTCGCAGCATTTGAAACGTACTGATACGACCACCGGCACGGGCAACTGTCGTCATTGTAGAAGAAGTAAATAACTCGGAGCCGCACACCACTACCATAATGACCCATAACGAGAACACGATTCCGCCGACTAATTTCGTCAACCCCCAGGa    <  1:155405/251‑1 (MQ=255)                                                                                                                                                                                                                             ccACTGCCCGTGAGCAGACATTGTCTGACCGGAAAACCAAATGACTAAAACAATAAAAATTGCGCCGACGAAATTACCACAATAAACTACAACCCAATTTCGCAGCATTTGAAACGTACTGATACGACCACCGGCACGGGCAACTGTCGTCATTGTAGAAGAAGTAAATAACTCGGAGCCGCACACCACTACCa                                                           <  1:193316/194‑1 (MQ=255)                                                                                                                                                                                                                             ccACTGCCCGTGAGCAGACATTGTCTGACCGGAAAACCAAATGACTAAAACAATAAAAATTGCGCCGACGAAATTACCACAATAAACTACAACCCAATTTCGCAGCATTTGAAACGTACTGATACGACCACCGGCACGGGCAACTGTCGTCATTGTAGAAGAAGTAAATAACTCGGAGCCGCACACCACTACCATAATGACCCCTAACGAGAACACGATCCCGCCGACTAATTTCGTCAACCCCCAGGAAc  <  1:833555/251‑1 (MQ=255)                                                                                                                                                                                                                                             ||                                                                                                                                                                                                                                          TCGGAATCATAAACATATTCGCCACACTGTGCTCAAACCCTGAGGCCACAAACAAAGCAATCGGTAAAATCATAATGAAGGCTTTATCTGTTAACGTTTTTCCCGCGTATGTCATCCAAATGGCAATACACACCATGATATTACATAAAATACCTAAGCAGAACGCTTCAATCCATGTATGGTGAATCTTATGTTGCGCCGTACTTAAAATGGTGAGCCCCCACTGCCCGTGAGCAACCATTGTCTGACCGGAAAACCAAATGACTAAAACAATAAAAATTGCGCCGACGAAATTACCACAATAAACTACAACCCAATTTCGCAGCATTTGAAACGTACTGATACGACCACCGGCACGGGCAACTGTCGTCATTGTAGAAGAAGTAAATAACTCGGAGCCGCATACCACTACCATAATGACCCCTAACGAGAACACGATCCCGCCGACTAATTTCGTCAACCCCCAGGAAC  >  1/2091566‑2092036

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑