Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,336,345	2 bp→GA	intergenic (+176765/+84591)	unknown → / ← unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,336,345	0	A	G	100.0%	132.7 / NA	48	intergenic (+176765/+84592)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (28/20);  total (28/20)
*	1	1,336,346	0	G	A	100.0%	127.5 / NA	48	intergenic (+176766/+84591)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (28/20);  total (28/20)

CGCAAACACCATTATCATCAATGCACCGATGTATAATTTTAATATCCCTACCCAATTAAAAAGCTATTTTGATTTCATTGCACGCCCTCGCGTAACATTTCAATATACGGAGGAAGGCCCTGAAGGATTATTAACAGGCAAAAAAGCCGTGGTATTAGCTGTATTCGGCGGGTTCCACCAAAATCAATCTTCCGATATCGTCACGGCATACTTAAAAATGATTTTAGGTTTCGTAGGTATTACCGATGTGCAATTCGTGTATGCAGAAGGCATTGGTTTTGGTCCTGAAGCGGTTGAAAAAGCACAAGCGCAAGCGAAAGCAGAAATAGATAAAATTGTGGCCGCACTTTAATCTCATAAATCTCATAAGAAACAAAAAAGCACCTCTAAGGTGCTTTTTTCATTTAAAGTGCAGTTACAAATAAAGTGCGGTCATAAAAAACAACATTTTTTATTAATGCAACTTTAATGATG  >  1/1336111‑1336584                                                                                                                                                                                                                                           ||                                                                                                                                                                                                                                               cGCAAACACCATTATCATCAATGCACTGATGTATAATTTTAATATCCCTACCCAATTAAAAAGCTATTTTGATTTCATTGCACGCCCTCGCGTAACATTTCAATATACGGAGGAAGGCCCTGAAGGATTATTAACAGGCAAAAAAGCCGTGGTATTAGCTGTATTCGGCGGGTTCCACCAAAATCAATCTTCCGATATCGTCACGGCATACTTAAAAATGATTTTAGGTTTCGTGAGTATTACCGAtgtg                                                                                                                                                                                                                                  >  1:678912/1‑250 (MQ=255)         ccATTATCATCAATGCACTGATGTATAATTTTAATATCCCTACCCAATTAAAAAGCTATTTTGATTTCATTGCACGCCCTCGCGTAACATTTCAATATACGGAGGAAGGCCCTGAAGGATTATTAACAGGCAAAAAAGCCGTGGTATTAGCTGTATTCGGCGGGTTCCACCAAAATCAATCTTCCGATATCGTCACGGCATACTTAAAAATGATTTTAGGTTTCGTGAGTATTACCGa                                                                                                                                                                                                                                      <  1:146708/238‑1 (MQ=255)         ccATTATCATCAATGCACTGATGTATAATTTTAATATCCCTACCCAATTAAAAAGCTATTTTGATTTCATTGCACGCCCTCGCGTAACATTTCAATATACGGAGGAAGGCCCTGAAGGATTATTAACAGGCAAAAAAGCCGTGGTATTAGCTGTATTCGGCGGGTTCCACCAAAATCAATCTTCCGATATCGTCACGGCATACTTAAAAATGATTTTAGGTTTCGTGAGTATTACCGATGTGCAATTCgtg                                                                                                                                                                                                                         >  1:337106/1‑251 (MQ=255)         ccATTATCATCAATGCACTGATGTATAATTTTAATATCCCTACCCAATTAAAAAGCTATTTTGATTTCATTGCACGCCCTCGCGTAACATTTCAATATACGGAGGAAGGCCCTGAAGGATTATTAACAGGCAAAAAAGCCGTGGTATTAGCTGTATTCGGCGGGTTCCACCAAAATCAATCTTCCGATATCGTCACGGCATACTTAAAAATGATTTTAGGTTTCGTGAGTATTACCGATGTGCAATTCgtg                                                                                                                                                                                                                         >  1:18809/1‑251 (MQ=255)                       gCACTGATGTATAATTTTAATATCCCTACCCAATTAAAAAGCTATTTTGATTTCATTGCACGCCCTCGCGTAACATTTCAATATACGGAGGAAGGCCCTGAAGGATTATTAACAGGCAAAAAAGCCGTGGTATTAGCTGTATTCGGCGGGTTCCACCAAAATCAATCTTCCGATATCGTCACGGCATACTTAAAAATGATTTTAGGTTTCGTGAGTATTACCGATGTGCAATTCGTGTATGCAGAAGGCAt                                                                                                                                                                                                           >  1:819573/1‑251 (MQ=255)                        cactgATGTATAATTTTAATATCCCTACCCAATTAAAAAGCTATTTTGATTTCATTGCACGCCCTCGCGTAACATTTCAATATACGGAGGAAGGCCCTGAAGGATTATTAACAGGCAAAAAAGCCGTGGTATTAGCTGTATTCGGCGGGTTCCACCAAAATCAATCTTCCGATATCGTCACGGCATACTTAAAAATGATTTTAGGTTTCGTGAGTATTACCGATGTGCAATTCGTGTATGCAGAAGGCAtt                                                                                                                                                                                                          <  1:405508/247‑1 (MQ=255)                            gATGTATAATTTTAATATCCCTACCCAATTAAAAAGCTATTTTGATTTCATTGCACGCCCTCGCGTAACATTTCAATATACGGAGGAAGGCCCTGAAGGATTATTAACAGGCAAAAAAGCCGTGGTATTAGCTGTATTCGGCGGGTTCCACCAAAATCAATCTTCCGATATCGTCACGGCATACTTAAAAATGATTTTAGGTTTCGTGAGTATTAc                                                                                                                                                                                                                                         <  1:70120/216‑1 (MQ=255)                            gATGTATAATTTTAATATCCCTACCCAATTAAAAAGCTATTTTGATTTCATTGCACGCCCTCGCGTAACATTTCAATATACGGAGGAAGGCCCTGAAGGATTATTAACAGGCAAAAAAGCCGTGGTATTAGCTGTATTCGGCGGGTTCCACCAAAATCAATCTTCCGATATCGTCACGGCATACTTAAAAATGATTTTAGGTTTCGTGAGTATTACCGATGTGCAATTCGTGTATGCAGAAGGCATTGGtt                                                                                                                                                                                                      >  1:714470/1‑251 (MQ=255)                            gATGTATAATTTTAATATCCCTACCCAATTAAAAAGCTATTTTGATTTCATTGCACGCCCTCGCGTAACATTTCAATATACGGAGGAAGGCCCTGAAGGATTATTAACAGGCAAAAAAGCCGTGGTATTAGCTGTATTCGGCGGGTTCCACCAAAATCAATCTTCCGATATCGTCACGGCATACTTAAAAATGATTTTAGGTTTCGTGAGTATTACCGATGTGCAATTCGTGTATGCAGAAGGCATTGGtt                                                                                                                                                                                                      >  1:761830/1‑251 (MQ=255)                            gATGTATAATTTTAATATCCCTACCCAATTAAAAAGCTATTTTGATTTCATTGCACGCCCTCGCGTAACATTTCAATATACGGAGGAAGGCCCTGAAGGATTATTAACAGGCAAAAAAGCCGTGGTATTAGCTGTATTCGGCGGGTTCCACCAAAATCAATCTTCCGATATCGTCACGGCATACTTAAAAATGATTTTAGGTTTCGTGAGTATTACCGATGTGCAATTCGTGTATGCAGAAGGCATTGGtt                                                                                                                                                                                                      >  1:675548/1‑251 (MQ=255)                                     ttttAATATCCCTACCCAATTAAAAAGCTATTTTGATTTCATTGCACGCCCTCGCGTAACATTTCAATATACGGAGGAAGGCCCTGAAGGATTATTAACAGGCAAAAAAGCCGTGGTATTAGCTGTATTCGGCGGGTTCCACCAAAATCAATCTTCCGATATCGTCACGGCATACTTAAAAATGATTTTAGGTTTCGTGAGTATTACCGATGTGCAATTCgtg                                                                                                                                                                                                                         >  1:862590/1‑223 (MQ=255)                                        tAATATCCCTACCCAATTAAAAAGCTATTTTGATTTCATTGCACGCCCTCGCGTAACATTTCAATATACGGAGGAAGGCCCTGAAGGATTATTAACAGGCAAAAAAGCCGTGGTATTAGCTGTATTCGGCGGGTTCCACCAAAATCAATCTTCCGATATCGTCACGGCATACTTAAAAATGATTTTAGGTTTCGTGAGTATTACCGATGTGCAATTCGTGTATGCAGAAGGCATTGGTTTTGGTCCTGAAg                                                                                                                                                                                          <  1:638180/251‑1 (MQ=255)                                             tCCCTACCCAATTAAAAAGCTATTTTGATTTCATTGCACGCCCTCGCGTAACATTTCAATATACGGAGGAAGGCCCTGAAGGATTATTAACAGGCAAAAAAGCCGTGGTATTAGCTGTATTCGGCGGGTTCCACCAAAATCAATCTTCCGATATCGTCACGGCATACTTAAAAATGATTTTAGGTTTCGTGAGTATTACCGATGTGCAATTCGTGTATGc                                                                                                                                                                                                                    <  1:545778/220‑1 (MQ=255)                                             tCCCTACCCAATTAAAAAGCTATTTTGATTTCATTGCACGCCCTCGCGTAACATTTCAATATACGGAGGAAGGCCCTGAAGGATTATTAACAGGCAAAAAAGCCGTGGTATTAGCTGTATTCGGCGGGTTCCACCAAAATCAATCTTCCGATATCGTCACGGCATACTTAAAAATGATTTTAGGTTTCGTGAGTATTACCGATGTGCAATTCGTGTATGCAGAAGGCATTGGTTTTGGTCCTGAAGCGGtt                                                                                                                                                                                     >  1:720601/1‑251 (MQ=255)                                                                         tttCATTGCACGCCCTCGCGTAACATTTCAATATACGGAGGAAGGCCCTGAAGGATTATTAACAGGCAAAAAAGCCGTGGTATTAGCTGTATTCGGCGGGTTCCACCAAAATCAATCTTCCGATATCGTCACGGCATACTTAAAAATGATTTTAGGTTTCGTGAGTATTACCGATGTGCAATTCGTGTATGc                                                                                                                                                                                                                    >  1:883824/1‑192 (MQ=255)                                                                              ttGCACGCCCTCGCGTAACATTTCAATATACGGAGGAAGGCCCTGAAGGATTATTAACAGGCAAAAAAGCCGTGGTATTAGCTGTATTCGGCGGGTTCCACCAAAATCAATCTTCCGATATCGTCACGGCATACTTAAAAATGATTTTAGGTTTCGTGAGTATTACCGATGTGCAATTCGTGTATGCAGAAGGCATTGGTTTTGGTCCTGAAGCGGTTGAAAAAGCACAAGCGCAAGCGAAAGCAGAAATa                                                                                                                                                    >  1:694492/1‑251 (MQ=255)                                                                              ttGCACGCCCTCGCGTAACATTTCAATATACGGAGGAAGGCCCTGAAGGATTATTAACAGGCAAAAAAGCCGTGGTATTAGCTGTATTCGGCGGGTTCCACCAAAATCAATCTTCCGATATCGTCACGGCATACTTAAAAATGATTTTAGGTTTCGTGAGTATTACCGATGTGCAATTCGTGTATGCAGAAGGCATTGGTTTTGGTCCTGAAGCGGTTGAAAAAGCACAAGCGCAAGCGAAAGCAGAAATa                                                                                                                                                    >  1:684991/1‑251 (MQ=255)                                                                                     cccTCGCGTAACATTTCAATATACGGAGGAAGGCCCTGAAGGATTATTAACAGGCAAAAAAGCCGTGGTATTAGCTGTATTCGGCGGGTTCCACCAAAATCAATCTTCCGATATCGTCACGGCATACTTAAAAATGATTTTAGGTTTCGTGAGTATTACCGATGTGCAATTCGTGTATGCAGAAGGCATTGGTTTTGGTCCTGAAGCGGTTGAAAAAGCACAAGCGCAAGCGAAAGCAGAAATAGATaaaa                                                                                                                                             >  1:14597/1‑251 (MQ=255)                                                                                       cTCGCGTAACATTTCAATATACGGAGGAAGGCCCTGAAGGATTATTAACAGGCAAAAAAGCCGTGGTATTAGCTGTATTCGGCGGGTTCCACCAAAATCAATCTTCCGATATCGTCACGGCATACTTAAAAATGATTTTAGGTTTCGTGAGTATTACCGATGTGc                                                                                                                                                                                                                                 <  1:383103/165‑1 (MQ=255)                                                                                        tCGCGTAACATTTCAATATACGGAGGAAGGCCCTGAAGGATTATTAACAGGCAAAAAAGCCGTGGTATTAGCTGTATTCGGCGGGTTCCACCAAAATCAATCTTCCGATATCGTCACGGCATACTTAAAAATGATTTTAGGTTTCGTGAGTATTACCGATGTGCAATTCGTGTATGCAGAAGGCATTGGTTTTGGTCCTGAAGCGGTTGAAAAAGCACAAGCGCAAGCGAAAGCAGAAATAGATAAAATtg                                                                                                                                          <  1:802045/251‑1 (MQ=255)                                                                                               aCATTTCAATATACGGAGGAAGGCCCTGAAGGATTATTAACAGGCAAAAAAGCCGTGGTATTAGCTGTATTCGGCGGGTTCCACCAAAATCAATCTTCCGATATCGTCACGGCATACTTAAAAATGATTTTAGGTTTCGTGAGTATTACCGATGTGCAATTCGTGTATGCAGAAGGCATTGGTTTTGGTCCTGAAGCGGTTGAAAAAGCACAAGCGCAAGCGAAAGCAGAAATAg                                                                                                                                                   <  1:542003/235‑1 (MQ=255)                                                                                                    tCAATATACGGAGGAAGGCCCTGAAGGATTATTAACAGGCAAAAAAGCCGTGGTATTAGCTGTATTCGGCGGGTTCCACCAAAATCAATCTTCCGATATCGTCACGGCATACTTAAAAATGATTTTAGGTTTCGTGAGTATTACCGATGTGCAATTCGTGTATGCAGAAGGCATTGGTTTTGGTCCTGAAGCGGTTGAAAAAGCACAAGCGCAAGCGAAAGCAGAAATAGATAAAATTGTGGCCGCACttt                                                                                                                              >  1:422281/1‑251 (MQ=255)                                                                                                    tCAATATACGGAGGAAGGCCCTGAAGGATTATTAACAGGCAAAAAAGCCGTGGTATTAGCTGTATTCGGCGGGTTCCACCAAAATCAATCTTCCGATATCGTCACGGCATACTTAAAAATGATTTTAGGTTTCGTGAGTATTACCGATGTGCAATTCGTGTATGCAGAAGGCATTGGTTTTGGTCCTGAAGCGGTTGAAAAAGCACAAGCGCAAGCGAAAGCAGAAATAGATAAAATTGTGGCCGCACttt                                                                                                                              >  1:224361/1‑251 (MQ=255)                                                                                                     cAATATACGGAGGAAGGCCCTGAAGGATTATTAACAGGCAAAAAAGCCGTGGTATTAGCTGTATTCGGCGGGTTCCACCAAAATCAATCTTCCGATATCGTCACGGCATACTTAAAAATGATTTTAGGTTTCGTGAGTATTACCGATGTGCAATTCGTGTATGCAGAAGGCATTGGTTTTGGTCCTGAAGCGGTTGAAAAAGCACAAGCGCAAGCGAAAGCAGAAATAGATAAAATTGTGGCCGCACTTTa                                                                                                                             >  1:207078/1‑251 (MQ=255)                                                                                                     cAATATACGGAGGAAGGCCCTGAAGGATTATTAACAGGCAAAAAAGCCGTGGTATTAGCTGTATTCGGCGGGTTCCACCAAAATCAATCTTCCGATATCGTCACGGCATACTTAAAAATGATTTTAGGTTTCGTGAGTATTACCGATGTGCAATTCGTGTATGCAGAAGGCATTGGTTTTGGTCCTGAAGCGGTTGAAAAAGCACAAGCGCAAGCGAAAGCAGAAATAGATAAAATTGTGGCCGCACTTTa                                                                                                                             >  1:245573/1‑251 (MQ=255)                                                                                                              gaggaAGGCCCTGAAGGATTATTAACAGGCAAAAAAGCCGTGGTATTAGCTGTATTCGGCGGGTTCCACCAAAATCAATCTTCCGATATCGTCACGGCATACTTAAAAATGATTTTAGGTTTCGTGAGTATTACCGATGTGCaa                                                                                                                                                                                                                               <  1:182495/144‑1 (MQ=255)                                                                                                                      cccTGAAGGATTATTAACAGGCAAAAAAGCCGTGGTATTAGCTGTATTCGGCGGGTTCCACCAAAATCAATCTTCCGATATCGTCACGGCATACTTAAAAATGATTTTAGGTTTCGTGAGTATTACCg                                                                                                                                                                                                                                       >  1:806392/1‑128 (MQ=255)                                                                                                                          gAAGGATTATTAACAGGCAAAAAAGCCGTGGTATTAGCTGTATTCGGCGGGTTCCACCAAAATCAATCTTCCGATATCGTCACGGCATACTTAAAAATGATTTTAGGTTTCGTGAGTATTACCGATGTGCAATTCGTGTATGCAGAAGGCATTGGTTTTGGTCCTGAAGCGGTTGAAAAAGCACAAGCGCAAGCGAAAGCAGAAATAGATAAAATTGTGGCCGCAc                                                                                                                                 <  1:394786/226‑1 (MQ=255)                                                                                                                                    taACAGGCAAAAAAGCCGTGGTATTAGCTGTATTCGGCGGGTTCCACCAAAATCAATCTTCCGATATCGTCACGGCATACTTAAAAATGATTTTAGGTTTCGTGAGTATTACCGATGTGCAATTCGTGTATGCAGAAGGCATTGGTTTTGGTCCTGAAGCGGTTGAAAAAGCACAAGCGCAAGCGAAAGCAGAAATAGATAAAATTGTGGCCGCACTTTAATCTCATAAATCTCATAAGAAACAAAAAAGc                                                                                              <  1:518237/251‑1 (MQ=255)                                                                                                                                          gCAAAAAAGCCGTGGTATTAGCTGTATTCGGCGGGTTCCACCAAAATCAATCTTCCGATATCGTCACGGCATACTTAAAAATGATTTTAGGTTTCGTGAGTATTACCGATGTGCAATTCGTGTATGCAGAAGGCATTGGTTTTGGTCCTGAAGCGGTTGAAAAAGCACAAGCGCAAGCGAAAGCAGAAATAGATAAAATTGTGGCCGCACTTTAATCTCATAAATCTCATAAGAAACAAAAAAGCAc                                                                                            <  1:835876/247‑1 (MQ=255)                                                                                                                                           cAAAAAAGCCGTGGTATTAGCTGTATTCGGCGGGTTCCACCAAAATCAATCTTCCGATATCGTCACGGCATACTTAAAAATGATTTTAGGTTTCGTGAGTATTACCGATGTGCAATTCGTGTATGCAGAAGGCATTGGTTTTGGTCCTGAAg                                                                                                                                                                                          >  1:359376/1‑152 (MQ=255)                                                                                                                                              aaaaGCCGTGGTATTAGCTGTATTCGGCGGGTTCCACCAAAATCAATCTTCCGATATCGTCACGGCATACTTAAAAATGATTTTAGGTTTCGTGAGTATTACCGATGTGCAATTCGTGTATGCAGAAGGCATTGGTTTTGGTCCTGAAGCGGTTGAAAAAGCACAAGCGCAAGCGAAAGCAGAAATAGATAAAATTGTGGCCGCACTTTAATCTCATAAATCTCATAAGAAACAAAAAAGCACCTCTAAgg                                                                                    <  1:261790/251‑1 (MQ=255)                                                                                                                                              aaaaGCCGTGGTATTAGCTGTATTCGGCGGGTTCCACCAAAATCAATCTTCCGATATCGTCACGGCATACTTAAAAATGATTTTAGGTTTCGTGAGTATTACCGATGTGCAATTCGTGTATGCAGAAGGCATTGGTTTTGGTCCTGAAGCGGTTGAAAAAGCACAAGCGCAAGCGAAAGCAGAAATAGATAAAATTGTGGCCGCACTTTAATCTCATAAATCTCATAAGAAACAAAAAAGCACCTCTAAgg                                                                                    <  1:62120/251‑1 (MQ=255)                                                                                                                                                       ggTATTAGCTGTATTCGGCGGGTTCCACCAAAATCAATCTTCCGATATCGTCACGGCATACTTAAAAATGATTTTAGGTTTCGTGAGTATTACCGATGTGCAATTCGTGTATGCAGAAGGCATTGGt                                                                                                                                                                                                       <  1:655996/127‑1 (MQ=255)                                                                                                                                                        gTATTAGCTGTATTCGGTGGGTTCCACCAAAATCAATCTTCCGATATCGTCACGGCATACTTAAAAATGATTTTAGGTTTCGTGAGTATTACCGATGTGCAATTCGTGTATGCAGAAGGCATTGGTTTTGGTCCTGAAGCGGTTGAAAAAGCACAAGCGCAAGCGAAAGCAGAAATAGATAAAATTGTGGCCGCACTTTAATCTCATAAATCTCATAAGAAACAAAAAAGCACCTCTAAGGTGCtttttt                                                                           >  1:238659/1‑250 (MQ=255)                                                                                                                                                                 gTATTCGGCGGGTTCCACCAAAATCAATCTTCCGATATCGTCACGGCATACTTAAAAATGATTTTAGGTTTCGTGAGTATTACCGATGTGCAATTCGTGTATGCAGAAGGCATTGGTTTTGGTCCTGAAGCGGTTGAAAAAGCAcaagcgcaagcg                                                                                                                                                                >  1:189874/1‑156 (MQ=255)                                                                                                                                                                   aTTCGGCGGGTTCCACCAAAATCAATCTTCCGATATCGTCACGGCATACTTAAAAATGATTTTAGGTTTCGTGAGTATTACCGATGTGCAATTCGTGTATGCAGAAGGCATTGGTTTTGGTCCTGAAGCGGTTGAAAAAGCACAAGCGCAAGCGAAAGCAGAAATAGATAAAATTGTGGCCGCACTTTAATCTCATAAATCTCATAAGAAACAAAAAAGCACCTCTAAgg                                                                                    <  1:518489/230‑1 (MQ=255)                                                                                                                                                                               ccaccaAAATCAATCTTCCGATATCGTCACGGCATACTTAAAAATGATTTTAGGTTTCGTGAGTATTACCGATGTGCAATTCGTGTATGCAGAAGGCATTGGTTTTGGTCCTGAAGCGGTTGAAAAAGCACAAGCGCAAGCGAAAGCAGAAATAGATAAAATTGTGGCCGCACTTTAATCTCATAAATCTCATAAGAAACAAAAAAGCACCTCTAAGGTGCTTTTTTCATTTAAAGTGCAGTTACAAATaa                                                   <  1:156431/251‑1 (MQ=255)                                                                                                                                                                                                   atatCGTCACGGCATACTTAAAAATGATTTTAGGCTTCGTGAGTATTACCGATGTGCAATTCGTGTATGCAGAAGGCATTGGTTTTGGTCCTGAAg                                                                                                                                                                                          >  1:712162/1‑96 (MQ=255)                                                                                                                                                                                                       cGTCACGGCATACTTAAAAATGATTTTAGGTTTCGTGAGTATTACCGATGTGCAATTCGTGTATGCAGAAGGCATTGGTTTTGGTCCTGAAGCGGTTGAAAAAGCACAAGCGCAAGCGAAAGCAGAAATAGATAAAATTGTGGCCGCACTTTAATCTCATAAATCTCATAAGAAACAAAAAAGCACCTCTAAGGTGCTTTTTTCATTTAAAGTGCAGTTACAAATAAAGTGCGGTCATAAAAAACAACAtt                           >  1:400981/1‑251 (MQ=255)                                                                                                                                                                                                            cGGCATACTTAAAAATGATTTTAGGTTTCGTGAGTATTACCGATGTGCAATTCGTGTATGCAGAAGGCATTGGTTTTGGTCCTGAAGCGGTTGAAAAAGCACAAGCGCAAGCGAAAGCAGAAATAGATAAAATTGTGGCCGCACTTTAATCTCATAAATCTCATAAGAAACAAAAAAGCACCTCTAAGGTGCTTTTTTCATTTAAAGTGCAGTTACAAATAAAGTGCGGTCATAAAAAACAACATTTTtta                      >  1:359849/1‑251 (MQ=255)                                                                                                                                                                                                            cGGCATACTTAAAAATGATTTTAGGTTTCGTGAGTATTACCGATGTGCAATTCGTGTATGCAGAAGGCATTGGTTTTGGTCCTGAAGCGGTTGAAAAAGCACAAGCGCAAGCGAAAGCAGAAATAGATAAAATTGTGGCCGCACTTTAATCTCATAAATCTCATAAGAAACAAAAAAGCACCTCTAAGGTGCTTTTTTCATTTAAAGTGCAGTTACAAATAAAGTGCGGTCATAAAAAACAACATTTTtta                      >  1:124153/1‑251 (MQ=255)                                                                                                                                                                                                              gCATACTTAAAAATGATTTTAGGTTTCGTGAGTATTACCGATGTGCAATTCGTGTATGCAGAAGGCATTGGTTTTGGTCCTGAAGCGGTTGAAAAAGCACAAGCGCAAGCGAAAGCAGAAATAGATAAAATTGTGGCCGCACTTTAATCTCATAAATCTCATAAGAAACAAAAAAGCACcttagagg                                                                                    <  1:542463/187‑7 (MQ=255)                                                                                                                                                                                                              gCATACTTAAAAATGATTTTAGGTTTCGTGAGTATTACCGATGTGCAATTCGTGTATGCAGAAGGCATTGGTTTTGGTCCTGAAGCGGTTGAAAAAGCACAAGCGCAAGCGAAAGCAGAAATAGATAAAATTGTGGCCGCACTTTAATCTCATAAATCTCATAAGAAACAAAAAAGCACCTCTAAGGTGCTTTTTTCATTTAAAGTGCAGTTACAAATAAAGTGCGGTCATAAAAAACAACATTTTttatt                    >  1:384010/1‑251 (MQ=255)                                                                                                                                                                                                                    ttAAAAATGATTTTAGGTTTCGTGAGTATTACCGATGTGCAATTCGTGTATGCAGAAGGCATTGGTTTTGGTCCTGAAGCGGTTGAAAAAGCACAAGCGCAAGCGAAAGCAGAAATAGATAAAATTGTGGCCGCACTTTAATCTCATAAATCTCATAAGAAACAAAAAAGCACCTCTAAGGTGCTTTTTTCATTTAAAGTGCAGTTACAAATAAAGTGCGGTCATAAAAAACAACATTTTTTATTAATGCa              >  1:203162/1‑251 (MQ=255)                                                                                                                                                                                                                             aTTTTAGGTTTCGTGAGTATTACCGATGTGCAATTCGTGTATGCAGAAGGCATTGGTTTTGGTCCTGAAg                                                                                                                                                                                          >  1:231284/1‑70 (MQ=255)                                                                                                                                                                                                                              ttttAGGTTTCGTGAGTATTACCGATGTGCAATTCGTGTATGCAGAAGGCATTGGTTTTGGTCCTGAAGCGGTTGAAAAAGCACAAGCGCAAGCGAAAGCAGAAATAGATAAAATTGTGGCCGCACTTTAATCTCATAAATCTCATAAGAAACAAAAAAGCACCTCTAAGGTGCTTTTTTCATTTAAAGTGCAGTTACAAATAAAGTGCGGTCATAAAAAACAACATTTTTTATTAATGCAACTTTAatga    <  1:497895/251‑1 (MQ=255)                                                                                                                                                                                                                                ttAGGTTTCGTGAGTATTACCGATGTGCAATTCGTGTATGCAGAAGGCATTGGTTTTGGTCCTGAAGCGGTTGAAAAAGCACAAGCGCAAGCGAAAGCAGAAATAGATAAAATTGTGGCCGCACTTTAATCTCATAAATCTCATAAGAAACAAAAAAGCACCTCTAAGGTGCTTTTTTCATTTAAAGTGCAGTTACAAATAAAGTGCGGTCATAAAAAACAACATTTTTTATTAATGCAACTTTAatgatg  <  1:591284/251‑1 (MQ=255)                                                                                                                                                                                                                                           ||                                                                                                                                                                                                                                               CGCAAACACCATTATCATCAATGCACCGATGTATAATTTTAATATCCCTACCCAATTAAAAAGCTATTTTGATTTCATTGCACGCCCTCGCGTAACATTTCAATATACGGAGGAAGGCCCTGAAGGATTATTAACAGGCAAAAAAGCCGTGGTATTAGCTGTATTCGGCGGGTTCCACCAAAATCAATCTTCCGATATCGTCACGGCATACTTAAAAATGATTTTAGGTTTCGTAGGTATTACCGATGTGCAATTCGTGTATGCAGAAGGCATTGGTTTTGGTCCTGAAGCGGTTGAAAAAGCACAAGCGCAAGCGAAAGCAGAAATAGATAAAATTGTGGCCGCACTTTAATCTCATAAATCTCATAAGAAACAAAAAAGCACCTCTAAGGTGCTTTTTTCATTTAAAGTGCAGTTACAAATAAAGTGCGGTCATAAAAAACAACATTTTTTATTAATGCAACTTTAATGATG  >  1/1336111‑1336584

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑