Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,204,243	3 bp→AAA	intergenic (+44663/+216692)	unknown → / ← unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,204,243	0	G	A	100.0%	68.7 / NA	27	intergenic (+44663/+216694)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (15/12);  total (15/12)
*	1	1,204,244	0	G	A	100.0%	67.1 / NA	27	intergenic (+44664/+216693)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (15/12);  total (15/12)
*	1	1,204,245	0	G	A	100.0%	66.2 / NA	27	intergenic (+44665/+216692)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (15/12);  total (15/12)

TTATCCGAAAATCTGGCAATC‑CTTATTGATTAGTTTTGTCGTCTGCCTACTGATTATGCTGAAACTCTTCTATCAGCCTAAAAAGAATCTTTATGGTAACGCCGAATTTGCGACTGCCTCGGAAGCGCGGGAAATGGGCTTGATGGAAGAAGAAAACGGCATTATCGTCGGCAAGCTCGGCAAAAAACTTTTACGCTTTATCGGTGCGCAATTCGTTTCACTGGCAGCGCCGACACGGGGAGGTAAAAGCGTAGGGATTGTTATTCCAAATTTGCTGGCATGGGAACAATCTGTGGTTGTGAAGGAC  >  1/1204115‑1204421                                                                                                                                  |||                                                                                                                                                                                 ttATCCGAAAATATGGCAGTCTCTCCTT‑ATTAGTTTTGTAGTGAGCTTACTAATCATGCTGAAACTATTCTATCAACCTAAAAAATCCCTTTATGGTAACGCTGAATTTGCCACAAAATCAGAAGTACAAAAAATGGGTTTATTGGAAGAAAAAAACGGGATTATTGTCGGCAAACTCGGTAATAAACTGCTCCGTTTTATCGGTGCGCAATTTGTTTCACTGGCAGCGCCGACACGGGGAGGTAAAAGCg                                                          <  1:637904/251‑1 (MQ=255) ttATCCGAAAATATGGCAGTCTCTCCTT‑ATTAGTTTTGTAGTGAGCTTACTAATCATGCTGAAACTATTCTATCAACCTAAAAAATCCCTTTATGGTAACGCTGAATTTGCCACAAAATCAGAAGTACAAAAAATGGGTTTATTGGAAGAAAAAAACGGGATTATTGTCGGCAAACTCGGTAATAAACTGCTCCGTTTTATCGGTGCGCAATTTGTTTCACTGGCAGCGCCGACACGGGGAGGTAAAAGCg                                                          <  1:561656/251‑1 (MQ=255)         aaaatatggcagtctctccttaTTAGTTTTGTAGTGAGCTTACTAATCATGCTGAAACTATTCTATCAACCTAAAAAATCCCTTTATGGTAACGCTGAATTTGCCACAAAATCAGAAGTACAAAAAATGGGTTTATTGGAAGAAAAAAACGGGATTATTGTCGGCAAACTCGGTAATAAACTGCTCCGTTTTATCGGTGCGCAATTTGTTTCACTGGCAGCGCCGACACGGGGAGGTAAAAGCGTAGGGa                                                    <  1:30421/229‑1 (MQ=255)         aaaatatggcagtctctccttaTTAGTTTTGTAGTGAGCTTACTAATCATGCTGAAACTATTCTATCAACCTAAAAAATCCCTTTATGGTAACGCTGAATTTGCCACAAAATCAGAAGTACAAAAAATGGGTTTATTGGAAGAAAAAAACGGGATTATTGTCGGCAAACTCGGTAATAAACTGCTCCGTTTTATCGGTGCGCAATTTGTTTCACTGGCAGCGCCGACACGGGGAGGTAAAAGCGTAGGGa                                                    <  1:37004/229‑1 (MQ=255)              atggcagtctctccttaTTAGTTTTGTAGTGAGCTTACTAATCATGCTGAAACTATTCTATCAACCTAAAAAATCCCTTTATGGTAACGCTGAATTTGCCACAAAATCAGAAGTACAAAAAATGGGTTTATTGGAAGAAAAAAACGGGATTATTGTCGGCAAACTCGGTAATAAACTGCTCCGTTTTATCGGTGCGCAATTTGTTTCACTGGCAGCGCCGACACGGGGAGGTAAAAGCGTAGGGATTGTTa                                              <  1:213856/235‑1 (MQ=255)                    gtctctccttaTTAGTTTTGTAGTGAGCTTACTAATCATGCTGAAACTATTCTATCAACCTAAAAAATCCCTTTATGGTAACGCTGAATTTGCCACAAAATCAGAAGTACAAAAAATGGGTTTATTGGAAGAAAAAAACGGGATTATTGTCGGCAAACTCGGTAATAAACTGCTCCGTTTTATCGGTGCGCAATTTGTTTCACTGGCAGCGCCGACACGGGGAGGTAAAAGCGTAGGGATTGTTATTCCaa                                        >  1:397716/11‑251 (MQ=255)                        ctccttaTTAGTTTTGTAGTGAGCTTACTAATCATGCTGAAACTATTCTATCAACCTAAAAAATCCCTTTATGGTAACGCTGAATTTGCCACAAAATCAGAAGTACAAAAAATGGGTTTATTGGAAGAAAAAAACGGGATTATTGTCGGCAAACTCGGTAATAAACTGCTCCGTTTTATCGGTGCGCAATTTGTTTCACTGGCAGCGCCGACACGGGGAGGTAAAAGCg                                                          >  1:665929/7‑229 (MQ=255)                          ccttaTTAGTTTTGTAGTGAGCTTACTAATCATGCTGAAACTATTCTATCAACCTAAAAAATCCCTTTATGGTAACGCTGAATTTGCCACAAAATCAGAAGTACAAAAAATGGGTTTATTGGAAGAAAAAAACGGGATTATTGTCGGCAAACTCGGTAATAAACTGCTCCGTTTTATCGGTGCGCAATTTGTTTCACTGGCAGCGCCGACACGGGGAGGTAAAAGc                                                           >  1:689161/5‑226 (MQ=255)                                  gttttgtagtgagcttactaatcaTGCTGAAACTATTCTATCAACCTAAAAAATCCCTTTATGGTAACGCTGAATTTGCCACAAAATCAGAAGTACAAAAAATGGGTTTATTGGAAGAAAAAAACGGGATTATTGTCGGCAAACTCGGTAATAAACTGCTCCGTTTTATCGGTGCGCAATTTGTTTCACTGGCAGCGCCGACACGGGGAGGTAAAAGCGTAGGGATTGTTATTCCAAATTTGCTGGCATgg                          >  1:89452/24‑251 (MQ=255)                                    tttgtagtgagcttactaatcaTGCTGAAACTATTCTATCAACCTAAAAAATCCCTTTATGGTAACGCTGAATTTGCCACAAAATCAGAAGTACAAAAAATGGGTTTATTGGAAGAAAAAAACGGGATTATTGTCGGCAAACTCGGTAATAAACTGCTCCGTTTTATCGGTGCGCAATTTGTTTCACTGGCAGCGCCGACACGGGGAGGCAAAAGCGTAGGGATTGTTATTCCAAATTTGCTGGCATGGGa                        <  1:640863/230‑1 (MQ=255)                                      tgtagtgagcttactaatcaTGCTGAAACTATTCTATCAACCTAAAAAATCCCTTTATGGTAACGCTGAATTTGCCACAAAATCAGAAGTACAAAAAATGGGTTTATTGGAAGAAAAAAACGGGATTATTGTCGGCAAACTCGGTAATAAACTGCTCCGTTTTATCGGTGCGCAATTTGTTTCACTGGCAGCGCCGACACGGGGAGGTAAAAGCGTAGGGATTGTTATTCCAAATTTGCTGGCATGGGAAc                      <  1:801555/232‑1 (MQ=255)                                      tgtagtgagcttactaatcaTGCTGAAACTATTCTATCAACCTAAAAAATCCCTTTATGGTAACGCTGAATTTGCCACAAAATCAGAAGTACAAAAAATGGGTTTATTGGAAGAAAAAAACGGGATTATTGTCGGCAAACTCGGTAATAAACTGCTCCGTTTTATCGGTGCGCAATTTGTTTCACTGGCAGCGCCGACACGGGGAGGTAAAAGCGTAGGGATTGTTATTCCAAATTTGCTGGCATGGGAAc                      <  1:271261/232‑1 (MQ=255)                                       gtagtgagcttactaatcaTGCTGAAACTATTCTATCAACCTAAAAAATCCCTTTATGGTAACGCTGAATTTGCCACAAAATCAGAAGTACAAAAAATGGGTTTATTGGAAGAAAAAAACGGGATTATTGTCGGCAAACTCGGTAATAAACTGCTCCGTTTTATCGGTGCGCAATTTGTTTCACTGGCAGCGCCGACACGGGGAGGTAAAAGCGTAGGGATTGTTATTCCAAATTTGCTGGCATGGGa                        >  1:495319/19‑248 (MQ=255)                                          gtgagcttactaatcaTGCTGAAACTATTCTATCAACCTAAAAAATCCCTTTATGGTAACGCTGAATTTGCCACAAAATCAGAAGTACAAAAAATGGGTTTATTGGAAGAAAAAAACGGGATTATTGTCGGCAAACTCGGTAATAAACTGCTCCGTTTTATCGGTGCGCAATTTGTTTCACTGGCAGCGCCGACACGGGGAGGTAAAAGc                                                           >  1:703339/16‑210 (MQ=255)                                            gagcttactaatcaTGCTGAAACTATTCTATCAACCTAAAAAATCCCTTTATGGTAACGCTGAATTTGCCACAAAATCAGAAGTACAAAAAATGGGTTTATTGGAAGAAAAAAACGGGATTATTGTCGGCAAACTCGGTAATAAACTGCTCCGTTTTATCGGTGCGCAATTTGTTTCACTGGCAGCGCCGACACGGGGAGGTAAAAGCGTAGGGATTGTTATTCCAAATTTGCTGGCATGGGAACAAt                   >  1:118435/14‑248 (MQ=255)                                            gagcttactaatcaTGCTGAAACTATTCTATCAACCTAAAAAATCCCTTTATGGTAACGCTGAATTTGCCACAAAATCAGAAGTACAAAAAATGGGTTTATTGGAAGAAAAAAACGGGATTATTGTCGGCAAACTCGGTAATAAACTGCTCCGTTTTATCGGTGCGCAATTTGTTTCACTGGCAGCGCCGACACGGGGAGGTAAAAGCGTAGGGATTGTTATTCCAAATTTGCTGGCATGGGAACAATCt                 >  1:459109/14‑250 (MQ=255)                                              gcttactaatcaTGCTGAAACTATTCTATCAACCTAAAAAATCCCTTTATGGTAACGCTGAATTTGCCACAAAATCAGAAGTACAAAAAATGGGTTTATTGGAAGAAAAAAACGGGATTATTGTCGGCAAACTCGGTAATAAACTGCTCCGTTTTATCGGTGCGCAATTTGTTTCACTGGCAGCGCCGACACGGGGAGGTAAAAGCGTAGGGAt                                                   >  1:806579/12‑214 (MQ=255)                                                ttactaatcaTGCTGAAACTATTCTATCAACCTAAAAAATCCCTTTATGGTAACGCTGAATTTGCCACAAAATCAGAAGTACAAAAAATGGGTTTATTGGAAGAAAAAGACGGGATTATTGTCGGCAAACTCGGTAATAAACTGCTCCGTTTTATCGGTGCGCAATTTGTTTCACTGGCAGCGCCGACACgggg                                                                     <  1:643501/185‑1 (MQ=255)                                                ttactaatcaTGCTGAAACTATTCTATCAACCTAAAAAATCCCTTTATGGTAACGCTGAATTTGCCACAAAATCAGAAGTACAAAAAATGGGTTTATTGGAAGAAAAAAACGGGATTATTGTCGGCAAACTCGGTAATAAACTGCTCCGTTTTATCGGTGCGCAATTTGTTTCACTGGCAGCGCCGACACGGGGAGGTaaa                                                              <  1:783674/192‑1 (MQ=255)                                                ttactaatcaTGCTGAAACTATTCTATCAACCTAAAAAATCCCTTTATGGTAACGCTGAATTTGCCACAAAATCAGAAGTACAAAAAATGGGTTTATTGGAAGAAAAAAACGGGATTATTGTCGGCAAACTCGGTAATAAACTGCTCCGTTTTATCGGTGCGCAATTTGTTTCACTGGCAGCGCCGACACGGGGAGGTAAAAGCGTAGGGATTGTTATTCCAAATTTGCTGGCATGGGAACAATCTGTGGt            <  1:185175/242‑1 (MQ=255)                                                 tactaatcaTGCTGAAACTATTCTATCAACCTAAAAAATCCCTTTATGGTAACGCTGAATTTGCCACAAAATCAGAAGTACAAAAAATGGGTTTATTGGAAGAAAAAAACGGGATTATTGTCGGCAAACTCGGTAATAAACTGCTCCGTTTTATCGGTGCGCAATTTGTTTCACTGGCAGCGCCGACACGGGGAGGTAAAAGCGTAGGGATTGTTATTCCAAATTTGCTGGCATGGGAAc                      >  1:830119/9‑240 (MQ=255)                                                 tactaatcaTGCTGAAACTATTCTATCAACCTAAAAAATCCCTTTATGGTAACGCTGAATTTGCCACAAAATCAGAAGTACAAAAAATGGGTTTATTGGAAGAAAAAAACGGGATTATTGTCGGCAAACTCGGTAATAAACTGCTCCGTTTTATCGGTGCGCAATTTGTTTCACTGGCAGCGCCGACACGGGGAGGTAAAAGCGTAGGGATTGTTATTCCAAATTTGCTGGCATGGGAACAATCTGTGGtt           >  1:617744/9‑251 (MQ=255)                                                          tGCTGAAACTATTCTATCAACCTAAAAAATCCCTTTATGGTAACGCTGAATTTGCCACAAAATCAGAAGTACAAAAAATGGGTTTATTGGAAGAAAAAAACGGGATTATTGTCGGCAAACTCGGTAATAAACTGCTCCGTTTTATCGGTGCGCAATTTGTTTCACTGGCAGCGCCGACACGGGGAGGTAAAAGCGTAGGGATTGTTATTCCAAATTTGCTGGCATGGGAACAATc                  >  1:205277/1‑235 (MQ=255)                                                          tGCTGAAACTATTCTATCAACCTAAAAAATCCCTTTATGGTAACGCTGAATTTGCCACAAAATCAGAAGTACAAAAAATGGGTTTATTGGAAGAAAAAAACGGGATTATTGTCGGCAAACTCGGTAATAAACTGCTCCGTTTTATCGGTGCGCAATTTGTTTCACTGGCAGCGCCGACACGGGGAGGTAAAAGCGTAGGGATTGTTATTCCAAATTTGCTGGCATGGGAACAATCTGTGGTTGTGAAGGa   >  1:173188/1‑250 (MQ=255)                                                          tGCTGAAACTATTCTATCAACCTAAAAAATCCCTTTATGGTAACGCTGAATTTGCCACAAAATCAGAAGTACAAAAAATGGGTTTATTGGAAGAAAAAAACGGGATTATTGTCGGCAAACTCGGTAATAAACTGCTCCGTTTTATCGGTGCGCAATTTGTTTCACTGGCAGCGCCGACACGGGGAGGTAAAAGCGTAGGGATTGTTATTCCAAATTTGCTGGCATGGGAACAATCTGTGGTTGTGAAGGa   >  1:53841/1‑250 (MQ=255)                                                          tGCTGAAACTATTCTATCAACCTAAAAAATCCCTTTATGGTAACGCTGAATTTGCCACAAAATCAGAAGTACAAAAAATGGGTTTATTGGAAGAAAAAAACGGGATTATTGTCGGCAAACTCGGTAATAAACTGCTCCGTTTTATCGGTGCGCAATTTGTTTCACTGGCAGCGCCGACACGGGGAGGTAAAAGCGTAGGGATTGTTATTCCAAATTTGCTGGCATGGGAACAATCTGTGGTTGTGAAGGa   >  1:874140/1‑250 (MQ=255)                                                          tGCTGAAACTATTCTATCAACCTAAAAAATCCCTTTATGGTAACGCTGAATTTGCCACAAAATCAGAAGTACAAAAAATGGGTTTATTGGAAGAAAAAAACGGGATTATTGTCGGCAAACTCGGTAATAAACTGCTCCGTTTTATCGGTGCGCAATTTGTTTCACTGGCAGCGCCGACACGGGGAGGTAAAAGCGTAGGGATTGTTATTCCAAATTTGCTGGCATGGGAACAATCTGTGGTTGTGAAGGAc  <  1:332126/251‑1 (MQ=255)                                                                                                                                  |||                                                                                                                                                                                 TTATCCGAAAATCTGGCAATC‑CTTATTGATTAGTTTTGTCGTCTGCCTACTGATTATGCTGAAACTCTTCTATCAGCCTAAAAAGAATCTTTATGGTAACGCCGAATTTGCGACTGCCTCGGAAGCGCGGGAAATGGGCTTGATGGAAGAAGAAAACGGCATTATCGTCGGCAAGCTCGGCAAAAAACTTTTACGCTTTATCGGTGCGCAATTCGTTTCACTGGCAGCGCCGACACGGGGAGGTAAAAGCGTAGGGATTGTTATTCCAAATTTGCTGGCATGGGAACAATCTGTGGTTGTGAAGGAC  >  1/1204115‑1204421

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑