Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,198,532	2 bp→AC	intergenic (+38952/+222404)	unknown → / ← unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,198,532	0	G	A	100.0%	140.5 / NA	52	intergenic (+38952/+222405)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (20/32);  total (20/32)
*	1	1,198,533	0	T	C	100.0%	138.2 / NA	52	intergenic (+38953/+222404)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (20/32);  total (20/32)

ATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCTAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAGAATTTACGTCAAGGTGAAATTACTTCCTGTGAAGCAAATATTGTTTCCACCGTGGAAAGTATGAGCTTCTTAGATTCGTTTGAAAGTAATGTCAATAGCAAACTTAAAGCAATTGATAAGTTAGCTAATCGTATGGCGAAATCAAACGATGTAAAAGAAAGCCAGGACTTAAAAAATGCAATGGACGTGCAGTTGGCCGCATTGCAATCACAAAAAGTCCAGTTGGATTTATTCAACGCACGGCAAACGGAATACCGACGCTTAATGAATGAGCAACAAAAACAGTTAGCGTCTCAACGTCGCTCAAACGTTAAGGGCGTTATATTCAAT  >  1/1198330‑1198742                                                                                                                                                                                                           ||                                                                                                                                                                                                                  aTTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGTTTCCACCGTGGAGAGTATGAGCTTCTTAGATTCGTTTGAAAGTAACGTCAATAGTAAATTGAAAACTATTGATAAACTCGCTAAACGGATGACAAAATCTCAAGATGTCAAAGAAAGCCAGGa                                                                                                                                                                     >  1:58629/1‑250 (MQ=255)       tttCCGGTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGTTTCCACCGTGGAGAGTATGAGCTTCTTAGATTCGTTTGAAAGTAACGTCAATAGTAAATTGAAAACTATTGATAAACTCGCTAAACGGATGACAAAATCTCAAGATGTCAAAGAAAGCCAGGACTTaaaa                                                                                                                                                              <  1:805680/251‑1 (MQ=255)             gTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGTTTCCACCGTGGAGAGTATGAGCTTCTTAGATTCGTTTGAAAGTAACGTCAATAGTAAATTGAAAACTATTGATAAACTCGCTAAACGGATGACAAAATCTCAAGATGTCAAAGAAAGCCAGGACTTAAAAAATGc                                                                                                                                                         <  1:482867/250‑1 (MQ=255)                         cATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCATTTCTTGTGAAGCAAATATTGTTTCCACCGTGGAGAGTATGAGCTTCTTAGATTCGTTTGAAAGTAACGTCAATAGTAAATTGAAAACTATTGATAAACTCGCTAAACGGATGACAAAATCTCAAGATGTCAAAGAAAGCCAGGACTTAAAAAATGCAATGGACGTGCAg                                                                                                                                            <  1:447650/251‑1 (MQ=255)                            tAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGTTTCCACCGTGGAGAGTATGAGCTTCTTAGATTCGTTTGAAAGTAACGTCAATAGTAAATTGAAAACTATTGATAAACTCGCTAAACGGATGACAAAATCTCAAGATGTCAAAGAAAGCCAGGACTTAAAAAATGCAATGGACGTGCAGTTg                                                                                                                                         <  1:671825/251‑1 (MQ=255)                                cgcgAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGTTTCCACCGTGGAGAGTATGAGCTTCTTAGATTCGTTTGAAAGTAACGTCAATAGTAAATTGAAAACTATTGATAAACTCGCTAAACGGATGACAAAATCTCAAGATGTCAAAGAAAGCCAGGACTTAAAAAATGCAATGGACGTGCAGTTGGCCg                                                                                                                                     >  1:746470/1‑251 (MQ=255)                                  cgAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGTTTCCACCGTGGAGAGTATGAGCTTCTTAGATTCGTTTGAAAGTAACGTCAATAGTAAATTGAAAACTATTGATAAACTCGCTAAACGGATGACAAAATCTCAAGATGTCAAAGAAAGCCAGGACTTAAAAAATGCAATGGACGTGCAGTTGGCCGc                                                                                                                                    <  1:405385/250‑1 (MQ=255)                                      tgccaaaaGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGTTTCCACCGTGGAGAGTATGAGCTTCTTAGATTCGTTTGAAAGTAACGTCAATAGTAAATTGAAAACTATTGATAAACTCGCTAAACGGATGACAAAATCTCAAGATGTCAAAGAAAGCCAGGACTTAAAAAATGCAATGGACGTGCAGTTGGCCGCATTGc                                                                                                                               <  1:827840/247‑1 (MQ=255)                                                cTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGTTTCCACCGTGGAGAGTATGAGCTTCTTAGATTCGTTTGAAAGTAACGTCAATAGTAAATTGAAAACTATTGATAAACTCGCTAAACGGATGACAAAATCTCAAGATGTCAAAGAAAGCCAGGACTTAAAAAATGCAATGGACGTGCAGTTGGCCGCATTGCAATCACaaaa                                                                                                                     <  1:880963/251‑1 (MQ=255)                                                             aCAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGTTTCCACCGTGGAGAGTATGAGCTTCTTAGATTCGTTTGAAAGTAACGTCAATAGTAAATTGAAAACTATTGATAAACTCGCTAAACGGATGACAAAATCTCAAGATGTCAAAGAAAGCCAGGACTTAAAAAATGCAATGGACGTGCAGTTGGCCGCATTGCAATCACAAAAAGTCCAGTTGGAt                                                                                                        <  1:49409/251‑1 (MQ=255)                                                               aaCCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGTTTCCACCGTGGAGAGTATGAGCTTCTTAGATTCGTTTGAAAGTAACGTCAATAGTAAATTGAAAACTATTGATAAACTCGCTAAACGGATGACAAAATCTCAAGATGTCAAAGAAAGCCAGGACTTAAAAAATGCAATGGACGTGCAGTTGGCCGCATTGCAATCACAAAAAGTCCAGTTGGAttt                                                                                                      <  1:252915/251‑1 (MQ=255)                                                                  cTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGTTTCCACCGTGGAGAGTATGAGCTTCTTAGATTCGTTTGAAAGTAACGTCAATAGTAAATTGAAAACTATTGATAAACTCGCTAAACGGATGACAAAATCTCAAGATGTCAAAGAAAGCCAGGACTTAAAAAATGCAATGGACGTGCAGTTGGCCGCATTGCAATCACAAAAAg                                                                                                                   >  1:269481/1‑235 (MQ=255)                                                                   tttttGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGTTTCCACCGTGGAGAGTATGAGCTTCTTAGATTCGTTTGAAAGTAACGTCAATAGTAAATTGAAAACTATTGATAAACTCGCTAAACGGATGACAAAATCTCAAGATGTCAAAGAAAGCCAGGACTTAAAAAATGCAATGGACGTGCAGTTGGCCGCATTGCAATCACAAAAAGTCCAGTTGGATTTATTc                                                                                                  <  1:190311/251‑1 (MQ=255)                                                                    ttttGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGTTTCCACCGTGGAGAGTATGAGCTTCTTAGATTCGTTTGAAAGTAACGTCAATAGTAAATTGAAAACTATTGATAAACTCGCTAAACGGATGACAAAATCTCAAGATGTCAAAGAAAGCCAGGAc                                                                                                                                                                    <  1:523565/184‑1 (MQ=255)                                                                                 aaaaaTTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGTTTCCACCGTGGAGAGTATGAGCTTCTTAGATTCGTTTGAAAGTAACGTCAATAGTAAATTGAAAACTATTGATAAACTCGCTAAACGGATGACAAAATCTCAAGATGTCAAAGAAAGCCAGGACTTAAAAAATGCAATGGACGTGCAGTTGGCCGCATTGCAATCACAAAAAGTCCAGTTGGATTTATTCAACGCACGGCAAAc                                                                                    <  1:645212/248‑1 (MQ=255)                                                                                            tCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGTTTCCACCGTGGAGAGTATGAGCTTCTTAGATTCGTTTGAAAGTAACGTCAATAGTAAATTGAAAACTATTGATAAACTCGCTAAACGGATGACAAAATCTCAAGATGTCAAAGAAAGCCAGGACTTAAAAAATGCAATGGACGTGCAGTTGGCCGCATTGCAATCACAAAAAGTCCAGTTGGATTTATTCAACGCACGGCAAACGGAATAccta                                                                          <  1:506760/250‑3 (MQ=255)                                                                                              aaGGTGAAATCACTTCTTGTGAAGCAAATATTGTTTCCACCGTGGAGAGTATGAGCTTCTTAGATTCGTTTGAAAGTAACGTCAATAGTAAATTGAAAACTATTGATAAACTCGCTAAACGGATGACAAAATCTCAAGATGTCAAAGAAAGCCAGGACTTAAAAAATGCAATGGACGTGCAGTTGGCCGCATTGCAATCACAAAAAGTCCAGTTGGATTTATTCAACGCACGGCAAACGGAATACCTACGc                                                                       <  1:772400/251‑1 (MQ=255)                                                                                              aaGGTGAAATCACTTCTTGTGAAGCAAATATTGTTTCCACCGTGGAGAGTATGAGCTTCTTAGATTCGTTTGAAAGTAACGTCAATAGTAAATTGAAAACTATTGATAAACTCGCTAAACGGATGACAAAATCTCAAGATGTCAAAGAAAGCCAGGACTTAAAAAATGCAATGGACGTGCAGTTGGCCGCATTGCAATCACAAAAAGTCCAGTTGGATTTATTCAACGCACGGCAAACGGAATACCTACGc                                                                       <  1:18114/251‑1 (MQ=255)                                                                                                 gTGAAATCACTTCTTGTGAAGCAAATATTGTTTCCACCGTGGAGAGTATGAGCTTCTTAGATTCGTTTGAAAGTAACGTCAATAGTAAATTGAAAACTATTGATAAACTCGCTAAACGGATGACAAAATCTCAAGATGTCAAAGAAAGCCAGGACTTAAAAAATGCAATGGACGTGCAGTTGGCCGCATTGCAATCACAAAAAGTCCAGTTGGATTTATTCAACGCACGGCAAACGGAATACCTACGCt                                                                      >  1:794316/1‑249 (MQ=255)                                                                                                    aaaTCACTTCTTGTGAAGCAAATATTGTTTCCACCGTGGAGAGTATGAGCTTCTTAGATTCGTTTGAAAGTAACGTCAATAGTAAATTGAAAACTATTGATAAACTCGCTAAACGGATGACAAAATCTCAAGATGTCAAAGAAAGCCAGGACTTAAAAAATGCAATGGACGTGCAGTTGGCCGCATTGCAATCACAAAAAGTCCAGTTGGATTTATTCAACGCACGGCAAACGGAATACCTACGCTTaatg                                                                 <  1:176915/251‑1 (MQ=255)                                                                                                    aaaTCACTTCTTGTGAAGCAAATATTGTTTCCACCGTGGAGAGTATGAGCTTCTTAGATTCGTTTGAAAGTAACGTCAATAGTAAATTGAAAACTATTGATAAACTCGCTAAACGGATGACAAAATCTCAAGATGTCAAAGAAAGCCAGGACTTAAAAAATGCAATGGACGTGCAGTTGGCCGCATTGCAATCACAAAAAGTCCAGTTGGATTTATTCAACGCACGGCAAACGGAATACCTACGCTTaat                                                                  <  1:619408/250‑1 (MQ=255)                                                                                                            tcttgtgAAGCAAATATTGTTTCCACCGTGGAGAGTATGAGCTTCTTAGATTCGTTTGAAAGTAACGTCAATAGTAAATTGAAAACTATTGATAAACTCGCTAAACGGATGACAAAATCTCAAGATGTCAAAGAAAGCCAGGACTTAAAAAATGCAATGGACGTGCAGTTGGCCGCATTGCAATCACAAAAAGTCCAGTTGGATTTATTCAACGCACGGCAAACGGAATACCTACGCTTAATGAATGAGca                                                         <  1:68357/248‑1 (MQ=255)                                                                                                                     gCAAATATTGTTTCCACCGTGGAGAGTATGAGCTTCTTAGATTCGTTTGAAAGTAACGTCAATAGTAAATTGAAAACTATTGATAAACTCGCTAAACGGATGACAAAATCTCAAGATGTCAAAGAAAGCCAGGACTTAAAAAATGCAATGGACGTGCAGTTGGCCGCATTGCAATCACAAAAAGTCCAGTTGGATTTATTCAACGCACGGCAAACGGAATACCTACGCTTAATGAATGAGCAACAAAAACa                                                >  1:407658/1‑251 (MQ=255)                                                                                                                      cAAATATTGTTTCCACCGTGGAGAGTATGAGCTTCTTAGATTCGTTTGAAAGTAACGTCAATAGTAAATTGAAAACTATTGATAAACTCGCTAAACGGATGACAAAATCTCAAGATGTCAAAGAAAGCCAGGACTTAAAAAATGCAATGGACGTGCAGt                                                                                                                                           <  1:554918/159‑1 (MQ=255)                                                                                                                      cAAATATTGTTTCCACCGTGGAGAGTATGAGCTTCTTAGATTCGTTTGAAAGTAACGTCAATAGTAAATTGAAAACTATTGATAAACTCGCTAAACGGATGACAAAATCTCAAGATGTCAAAGAAAGCCAGGACTTAAAAAATGCAATGGACGTGCAGTTGGCCGCATTGCAATCACAAAAAGTCCAGTTGGATTTATTCAACGCACGGCAAACGGAATACCTACGCTTAATGAATGAGCAACAAAAACAg                                               <  1:879705/251‑1 (MQ=255)                                                                                                                         atatTGTTTCCACCGTGGAGAGTATGAGCTTCTTAGATTCGTTTGAAAGTAACGTCAATAGTAAATTGAAAACTATTGATAAACTCGCTAAACGGATGACAAAATCTCAAGATGTCAAAGAAAGCCAGGACTTAAAAAATGCAATGGACGTGCAGTTGGCCGCATTGCAATCACAAAAAGTCCAGTTGGATTTATTCAACGCACGGCAAACGGAATACCTACGCTTAATGAATGAGCAACAAAAACAGTTa                                            >  1:557417/1‑251 (MQ=255)                                                                                                                          tatTGTTTCCACCGTGGAGAGTATGAGCTTCTTAGATTCGTTTGAAAGTAACGTCAATAGTAAATTGAAAACTATTGATAAACTCGCTAAACGGATGACAAAATCTCAAGATGTCAAAGAAAGCCAGGACTTaaaa                                                                                                                                                              >  1:721711/1‑136 (MQ=255)                                                                                                                          tatTGTTTCCACCGTGGAGAGTATGAGCTTCTTAGATTCGTTTGAAAGTAACGTCAATAGTAAATTGAAAACTATTGATAAACTCGCTAAACGGATGACAAAATCTCAAGATGTCAAAGAAAGCCAGGACTTAAAAAATGCAATGGACGTGCAGTTGGCCGCATTGCAATCACAAAAAGTCCAGTTGGATTTATTCAACGCACGGCAAACGGAATACCTACGCTTAATGAATGAGCAACAAAAACAGTTAg                                           <  1:778239/251‑1 (MQ=255)                                                                                                                            ttGTTTCCACCGTGGAGAGTATGAGCTTCTTAGATTCGTTTGAAAGTAACGTCAATAGTAAATTGAAAACTATTGATAAACTCGCTAAACGGATGACAAAATCTCAAGATGTCAAAGAAAGCCAGGACTTAAAAAATGCAATGGACGTGCAGTTGGCCGCATTGCAATCACAAAAAGTCCAGTTGGATTTATTCAACGCACGGCAAACGGAATACCTACGCTTAATGAATGAGCAACAAAAACAGTTAGCg                                         >  1:482896/1‑251 (MQ=255)                                                                                                                            ttGTTTCCACCGTGGAGAGTATGAGCTTCTTAGATTCGTTTGAAAGTAACGTCAATAGTAAATTGAAAACTATTGATAAACTCGCTAAACGGATGACAAAATCTCAAGATGTCAAAGAAAGCCAGGACTTAAAAAATGCAATGGACGTGCAGTTGGCCGCATTGCAATCACAAAAAGTCCAGTTGGATTTATTCAACGCACGGCAAACGGAATACCTACGCTTAATGAATGAGCAACAAAAACAGTTAGCg                                         >  1:95732/1‑251 (MQ=255)                                                                                                                                  ccACCGTGGAGAGTATGAGCTTCTTAGATTCGTTTGAAAGTAACGTCAATAGTAAATTGAAAACTATTGATAAACTCGCTAAACGGATGACAAAATCTCAAGATGTCAAAGAAAGCCAGGACTTAAAAAATGCAATGGACGTGCAGTTGGc                                                                                                                                       <  1:520275/151‑1 (MQ=255)                                                                                                                                   cACCGTGGAGAGTATGAGCTTCTTAGATTCGTTTGAAAGTAACGTCAATAGTAAATTGAAAACTATTGATAAACTCGCTAAACGGATGACAAAATCTCAAGATGTCAAAGAAAGCCAGGACTTAAAAAATGCAATGGACGTGCAGTTGGCCGCATTGCAATCACAAAAAGTCCAGTTGGATTTATTCAACGCACGGCAAACGGAATACCTACGCTTAATGAATGAGCAACAAAAACAGTTAGCGTCTCAAc                                  <  1:361700/251‑1 (MQ=255)                                                                                                                                    aCCGTGGAGAGTATGAGCTTCTTAGATTCGTTTGAAAGTAACGTCAATAGTAAATTGAAAACTATTGATAAACTCGCTAAACGGATGACAAAATCTCAAGATGTCAAAGAAAGCCAGGACTTAAAAAATGCAATGGACGTGCAGTTGGCCGCATTGCaa                                                                                                                             <  1:515217/159‑1 (MQ=255)                                                                                                                                    aCCGTGGAGAGTATGAGCTTCTTAGATTCGTTTGAAAGTAACGTCAATAGTAAATTGAAAACTATTGATAAACTCGCTAAACGGATGACAAAATCTCAAGATGTCAAAGAAAGCCAGGACTTAAAAAATGCAATGGACGTGCAGTTGGCCGCATTGCAATCACAAAAAGTCCAGTTGGATTTATTCAACGCACGGCAAACGGAATACCTACGCTTaatg                                                                 <  1:246456/219‑1 (MQ=255)                                                                                                                                    aCCGTGGAGAGTATGAGCTTCTTAGATTCGTTTGAAAGTAACGTCAATAGTAAATTGAAAACTATTGATAAACTCGCTAAACGGATGACAAAATCTCAAGATGTCAAAGAAAGCCAGGACTTAAAAAATGCAATGGACGTGCAGTTGGCCGCATTGCAATCACAAAAAGTCCAGTTGGATTTATTCAACGCACGGCAAACGGAATACCTACGCTTAATGAATGAGCAACAAAAACAGTTAGCGTCTCAACg                                 >  1:549341/1‑251 (MQ=255)                                                                                                                                    aCCGTGGAGAGTATGAGCTTCTTAGATTCGTTTGAAAGTAACGTCAATAGTAAATTGAAAACTATTGATAAACTCGCTAAACGGATGACAAAATCTCAAGATGTCAAAGAAAGCCAGGACTTAAAAAATGCAATGGACGTGCAGTTGGCCGCATTGCAATCACAAAAAGTCCAGTTGGATTTATTCAACGCACGGCAAACGGAATACCTACGCTTAATGAATGAGCAACAAAAACAGTTAGCGTCTCAACg                                 >  1:592759/1‑251 (MQ=255)                                                                                                                                    aCCGTGGAGAGTATGAGCTTCTTAGATTCGTTTGAAAGTAACGTCAATAGTAAATTGAAAACTATTGATAAACTCGCTAAACGGATGACAAAATCTCAAGATGTCAAAGAAAGCCAGGACTTAAAAAATGCAATGGACGTGCAGTTGGCCGCATTGCAATCACAAAAAGTCCAGTTGGATTTATTCAACGCACGGCAAACGGAATACCTACGCTTAATGAATGAGCAACAAAAACAGTTAGCGTCTCAACg                                 >  1:396846/1‑251 (MQ=255)                                                                                                                                     ccGTGGAGAGTATGAGCTTCTTAGATTCGTTTGAAAGTAACGTCAATAGTAAATTGAAAACTATTGATAAACTCGCTAAACGGATGACAAAATCTCAAGATGTCAAAGAAAGCCAGGACTTAAAAAATGCAATGGACGTGCAGTTGGCCGCATTGCAATCACAAAAAGTCCAGTTGGATTTATTCAACGCACGGCAAACGGAATACCTACGCTTAATGAATGAGCAACAAAAACAGTTAGCGTCTCAACGt                                >  1:295796/1‑251 (MQ=255)                                                                                                                                            gaGTATGAGCTTCTTAGATTCGTTTGAAAGTAACGTCAATAGTAAATTGAAAACTATTGATAAACTCGCTAAACGGATGACAAAATCTCAAGATGTCAAAGAAAGCCAGGACTTAAAAAATGCAATGGACGTGCAGTTGGCCGCATTGCAATCACAAAAAGTCCAGTTGGATTTATTCAACGCACGGCAAACGGAATACCTACGCTTAATGAATGAGCAACAAAAACAGTTAGCGTCTCAACGTCGCttaa                         >  1:572981/2‑248 (MQ=255)                                                                                                                                              gTATGAGCTTCTTAGATTCGTTTGAAAGTAACGTCAATAGTAAATTGAAAACTATTGATAAACTCGCTAAACGGATGACAAAATCTCAAGATGTCAAAGAAAGCCAGGACTTAAAAAATGCAATGGACGTGCAGTTGGCCGCATTGCaa                                                                                                                             <  1:4547/149‑1 (MQ=255)                                                                                                                                              gTATGAGCTTCTTAGATTCGTTTGAAAGTAACGTCAATAGTAAATTGAAAACTATTGATAAACTCGCTAAACGGATGACAAAATCTCAAGATGTCAAAGAAAGCCAGGACTTAAAAAATGCAATGGACGTGCAGTTGGCCGCATTGCAATCACAAAAAGTCCAGTTGGAt                                                                                                        <  1:614596/170‑1 (MQ=255)                                                                                                                                              gTATGAGCTTCTTAGATTCGTTTGAAAGTAACGTCAATAGTAAATTGAAAACTATTGATAAACTCGCTAAACGGATGACAAAATCTCAAGATGTCAAAGAAAGCCAGGACTTAAAAAATGCAATGGACGTGCAGTTGGCCGCATTGCAATCACAAAAAGTCCAGTTGGATTTATTCAACGCACGGCAAACGGAATACCTACGCTTAATGAATGAGCAACAAAAACAGTTAGCGTCTCAACGTCGCTTaaa                        >  1:146889/1‑250 (MQ=255)                                                                                                                                              gTATGAGCTTCTTAGATTCGTTTGAAAGTAACGTCAATAGTAAATTGAAAACTATTGATAAACTCGCTAAACGGATGACAAAATCTCAAGATGTCAAAGAAAGCCAGGACTTAAAAAATGCAATGGACGTGCAGTTGGCCGCATTGCAATCACAAAAAGTCCAGTTGGATTTATTCAACGCACGGCAAACGGAATACCTACGCTTAATGAATGAGCAACAAAAACAGTTAGCGTCTCAACGTCGCTTaaa                        >  1:322559/1‑250 (MQ=255)                                                                                                                                                     cttcttAGATTCGTTTGAAAGTAACGTCAATAGTAAATTGAAAACTATTGATAAACTCGCTAAACGGATGACAAAATCTCAAGATGTCAAAGAAAGCCAGGACTTAAAAAATGCAATGGACGTGCAGTTGGCCGCATTGCAATCACAAAAAGTCCAGTTGGATTTATTCAACGCACGGCAAACGGAATACCTACGCTTAATGAATGAGCAACAAAAACAGTTAGCGTCTCAACGTCGCTTAAACGTTAAg                 <  1:427744/250‑1 (MQ=255)                                                                                                                                                       tcttAGATTCGTTTGAAAGTAACGTCAATAGTAAATTGAAAACTATTGATAAACTCGCTAAACGGATGACAAAATCTCAAGATGTCAAAGAAAGCCAGGACTTAAAAAATGCAATGGACGTGCAGTTGGCCGCATTGCAATCACAAAAAGTCCAGTTGGATTTATTCAACGCACGGCAAACGGAATAc                                                                             >  1:214849/1‑188 (MQ=255)                                                                                                                                                       tcttAGATTCGTTTGAAAGTAACGTCAATAGTAAATTGAAAACTATTGATAAACTCGCTAAACGGATGACAAAATCTCAAGATGTCAAAGAAAGCCAGGACTTAAAAAATGCAATGGACGTGCAGTTGGCCGCATTGCAATCACAAAAAGTCCAGTTGGATTTATTCAACGCACGGCAAACGGAATACCTACGCTTAATGAATGAGCAACAAAAACAGTTAGCGTCTCAACGTCGCTTAAACGTTAAggg               >  1:364191/1‑250 (MQ=255)                                                                                                                                                       tcttAGATTCGTTTGAAAGTAACGTCAATAGTAAATTGAAAACTATTGATAAACTCGCTAAACGGATGACAAAATCTCAAGATGTCAAAGAAAGCCAGGACTTAAAAAATGCAATGGACGTGCAGTTGGCCGCATTGCAATCACAAAAAGTCCAGTTGGATTTATTCAACGCACGGCAAACGGAATACCTACGCTTAATGAATGAGCAACAAAAACAGTTAGCGTCTCAACGTCGCTTAAACGTTAAggg               >  1:14967/1‑250 (MQ=255)                                                                                                                                                                cGTTTGAAAGTAACGTCAATAGTAAATTGAAAACTATTGATAAACTCGCTAAACGGATGACAAAATCTCAAGATGTCAAAGAAAGCCAGGACTTAAAAAATGCAATGGACGTGCAGTTGGCCGCATTGCAATCACAAAAAGTCCAGTTGGATTTATTCAACGCACGGCAAACGGAATACCTACGCTTAATGAATGAGCAACAAAAACAGTTAGCGTCTCAACGTCGCTTAAACGTTAAGGGCGTTATAtt      >  1:517490/1‑250 (MQ=255)                                                                                                                                                                 gTTTGAAAGTAACGTCAATAGTAAATTGAAAACTATTGATAAACTCGCTAAACGGATGACAAAATCTCAAGATGTCAAAGAAAGCCAGGACTTAAAAAATGCAATGGACGTGCAGTTGGCCGCATTGCAATCACAAAAAGTCCAGTTGGATTTATTCAACGCACGGCAAACGGAATACCTACGCTTAATGAATGAGCAACAAAAACAGTTAGCGTCTCAACGTCGCTTAAACGTTAAGGGCGTTATATTc     <  1:548839/250‑1 (MQ=255)                                                                                                                                                                  tttGAAAGTAACGTCAATAGTAAATTGAAAACTATTGATAAACTCGCTAAACGGATGACAAAATCTCAAGATGTCAAAGAAAGCCAGGACTTAAAAAATGCAATGGACGTGCAGTTGGCCGCATTGCAATCACAAAAAGTCCAGTTGGATTTATTCAACGCACGGCAAACGGAATACCTACGCTTAATGAATGAGCAACAAAAACAGTTAGCGTCTCAACGt                                <  1:883047/222‑1 (MQ=255)                                                                                                                                                                   ttGAAAGTAACGTCAATAGTAAATTGAAAACTATTGATAAACTCGCTAAACGGATGACAAAATCTCAAGATGTCAAAGAAAGCCAGGACTTAAAAAATGCAATGGACGTGCAGTTGGCCGCATTGCAATCACAAAAAGTCCAGTTGGATTTATTCAACGCACGGCAAACGGAATACCTACGCTTAATGAATGAGCAACAAAAACAGTTAGCGTCTCAACGTCGCTTAAACGTTAAGGGCGTTATATTCaac  <  1:806231/251‑2 (MQ=255)                                                                                                                                                                   ttGAAAGTAACGTCAATAGTAAATTGAAAACTATTGATAAACTCGCTAAACGGATGACAAAATCTCAAGATGTCAAAGAAAGCCAGGACTTAAAAAATGCAATGGACGTGCAGTTGGCCGCATTGCAATCACAAAAAGTCCAGTTGGATTTATTCAACGCACGGCAAACGGAATACCTACGCTTAATGAATGAGCAACAAAAACAGTTAGCGTCTCAACGTCGCTTAAACGTTAAGGGCGTTATATTCaac  <  1:256227/251‑2 (MQ=255)                                                                                                                                                                                                           ||                                                                                                                                                                                                                  ATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCTAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAGAATTTACGTCAAGGTGAAATTACTTCCTGTGAAGCAAATATTGTTTCCACCGTGGAAAGTATGAGCTTCTTAGATTCGTTTGAAAGTAATGTCAATAGCAAACTTAAAGCAATTGATAAGTTAGCTAATCGTATGGCGAAATCAAACGATGTAAAAGAAAGCCAGGACTTAAAAAATGCAATGGACGTGCAGTTGGCCGCATTGCAATCACAAAAAGTCCAGTTGGATTTATTCAACGCACGGCAAACGGAATACCGACGCTTAATGAATGAGCAACAAAAACAGTTAGCGTCTCAACGTCGCTCAAACGTTAAGGGCGTTATATTCAAT  >  1/1198330‑1198742

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑